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Results for “SHEARING STRESS”

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At least 73 records · Page 4
All sourcesNASA NTRSDOE OSTI
NASA NTRS1978

Buried wire gage for wall shear stress measurements

A buried wire gage for measuring wall shear stress in fluid flow was studied and further developed. Several methods of making this relatively new type of gage were examined to arrive at a successful technique that is well-suited for wind-tunnel testing. A series of measurements was made to demonstrate the adequacy of a two-point calibration procedure for these gages. The buried wire gage is also demonstrated to be ideally suited for quantitative measurement of wall shear stress in wind-tunnel testing.

Murthy, V. S.↗
NASA NTRS2013

Shear Stress Sensing using Elastomer Micropillar Arrays

The measurement of shear stress developed as a fluid moves around a solid body is difficult to measure. Stresses at the fluid-solid interface are very small and the nature of the fluid flow is easily disturbed by introducing sensor components to the interface. To address these challenges, an array of direct and indirect techniques have been investigated with various advantages and challenges. Hot wire sensors and other indirect sensors all protrude significantly into the fluid flow. Microelectromechanical systems (MEMS) devices, although facilitating very accurate measurements, are not durable, are prone to contamination, and are difficult to implement into existing model geometries. One promising approach is the use of engineered surfaces that interact with fluid flow in a detectable manner. To this end, standard lithographic techniques have been utilized to generate elastomeric micropillar arrays of various lengths and diameters. Micropillars of controlled length and width were generated in polydimethylsiloxane (PDMS) elastomer using a soft-lithography technique. The 3D mold for micropillar replication was fabricated using laser ablative micromachining and contact lithography. Micropillar dimensions and mechanical properties were characterized and compared to shear sensing requirements. The results of this characterization as well as shear stress detection techniques will be discussed.

Wohl, Christopher J.↗
NASA NTRS1997

Intracellular pH changes in human aortic smooth muscle cells in response to fluid shear stress

The smooth muscle cell (SMC) layers of human arteries may be exposed to blood flow after endothelium denudation, for example, following balloon angioplasty treatment. These SMCs are also constantly subjected to pressure driven transmural fluid flow. Flow-induced shear stress can alter SMC growth and metabolism. Signal transduction mechanisms involved in these flow effects on SMCs are still poorly understood. In this work, the hypothesis that shear stress alters the intracellular pH (pHi) of SMC is examined. When exposed to venous and arterial levels of shear stress, human aortic smooth muscle cells (hASMC) undergo alkalinization. The alkalinization plateau persisted even after 20 min of cell exposure to flow. Addition of amiloride (10 micromoles) or its 5-(N-ethyl-N-isopropyl) analog (EIPA, 10 micromoles), both Na+/H+ exchanger inhibitors, attenuated intracellular alkalinization, suggesting the involvement of the Na+/H+ exchanger in this response. The same concentrations of these inhibitors did not show an effect on pHi of hASMCs in static culture. 4-Acetamido-4'-isothio-cyanatostilbene-2,2'-disulfonic acid (SITS, 1 mM), a Cl-/HCO3- exchange inhibitor, affected the pHi of hASMCs both in static and flow conditions. Our results suggest that flow may perturb the Na+/H+ exchanger leading to an alkalinization of hASMCs, a different response from the flow-induced acidification seen with endothelial cells at the same levels of shear stress. Understanding the flow-induced signal transduction pathways in the vascular cells is of great importance in the tissue engineering of vascular grafts. In the case of SMCs, the involvement of pHi changes in nitric oxide production and proliferation regulation highlights further the significance of such studies.

NASA Discipline Cell Biology↗
NASA NTRS2002

Shear stress stimulates phosphorylation of endothelial nitric-oxide synthase at Ser1179 by Akt-independent mechanisms: role of protein kinase A

Recently, we have shown that shear stress stimulates NO(*) production by the protein kinase B/Akt (Akt)-dependent mechanisms in bovine aortic endothelial cells (BAEC) (Go, Y. M., Boo, Y. C., Park, H., Maland, M. C., Patel, R., Pritchard, K. A., Jr., Fujio, Y., Walsh, K., Darley-Usmar, V., and Jo, H. (2001) J. Appl. Physiol. 91, 1574-1581). Akt has been believed to regulate shear-dependent production of NO(*) by directly phosphorylating endothelial nitric-oxide synthase (eNOS) at the Ser(1179) residue (eNOS-S(1179)), but a critical evaluation using specific inhibitors or dominant negative mutants (Akt(AA) or Akt(AAA)) has not been reported. In addition, other kinases, including protein kinase A (PKA) and AMP kinase have also shown to phosphorylate eNOS-S(1179). Here, we show that shear-dependent phosphorylation of eNOS-S(1179) is mediated by an Akt-independent, but a PKA-dependent, mechanism. Expression of Akt(AA) or Akt(AAA) in BAEC by using recombinant adenoviral constructs inhibited phosphorylation of eNOS-S(1179) if cells were stimulated by vascular endothelial growth factor (VEGF), but not by shear stress. As shown before, expression of Akt(AA) inhibited shear-dependent NO(*) production, suggesting that Akt is still an important regulator in NO production. Further studies showed that a selective inhibitor of PKA, H89, inhibited shear-dependent phosphorylation of eNOS-S(1179) and NO(*) production. In contrast, H89 did not inhibit phosphorylation of eNOS-S(1179) induced by expressing a constitutively active Akt mutant (Akt(Myr)) in BAEC, showing that the inhibitor did not affect the Akt pathway. 8-Bromo-cAMP alone phosphorylated eNOS-S(1179) within 5 min without activating Akt, in an H89-sensitive manner. Collectively, these results demonstrate that shear stimulates phosphorylation of eNOS-S(1179) in a PKA-dependent, but Aktindependent manner, whereas the NO(*) production is regulated by the mechanisms dependent on both PKA and Akt. A coordinated interaction between Akt and PKA may be an important mechanism by which eNOS activity is regulated in response to physiological stimuli such as shear stress.

Non-NASA Center↗
NASA NTRS2010

A Two-Axis Direct Fluid Shear Stress Sensor

This innovation is a miniature or micro sized semiconductor sensor design that provides two axis direct non-intrusive measurement of skin friction or wall shear stress in fluid flow. The sensor is fabricated by micro-electro-mechanical system (MEMS) technology, enabling small size and low cost reproductions. The sensors have been fabricated by utilizing MEMS fabrication processes to bond a sensing element wafer to a fluid coupling wafer. This layering technique provides for an out of plane dimension that is on the same order of length as the inplane dimensions. The sensor design has the following characteristics: a shear force collecting plate with dimensions that can be tailored to various application specific requirements such as spatial resolution, temporal resolution and shear force range and resolution. This plate is located coplanar to both the sensor body and flow boundary, and is connected to a dual axis gimbal structure by a connecting column or lever arm. The dual axis gimbal structure has torsional hinges with embedded piezoresistive torsional strain gauges which provide a voltage output that is correlated to the applied shear stress (and excitation current) on force collection plate that is located on the flow boundary surface (hence the transduction method). This combination of design elements create a force concentration and resolution structure that enables the generation of a large stress on the strain gauge from the small shear stress on the flow boundary wall. This design as well as the use of back side electrical contacts establishes a non-intrusive method to quantitatively measure the shear force vector on aerodynamic bodies.

Adcock, Edward E.↗
NASA NTRS1945

Bending and shear stresses developed by the instantaneous arrest of the root of a cantilever beam rotating with constant angular velocity about a transverse axis through the root

A theoretical investigation was made of the behavior of a cantilever beam in rotational motion about a transverse axis through the root determining the stresses, the deflections, and the accelerations that occur in the beam as a result of the arrest of motion. The equations for bending and shear stress reveal that, at a given percentage of the distance from root to tip and at a given trip velocity, the bending stresses for a particular mode are independent of the length of the beam and the shear stresses vary inversely with the length. When examined with respect to a given angular velocity instead of a given tip velocity, the equations reveal that the bending stress is proportional to the length of the beam whereas the shear stress is independent of the length. Sufficient experimental verification of the theory has previously been given in connection with another problem of the same type.

BEAMS - VIBRATION↗
NASA NTRS2013

Recalibration of the Shear Stress Transport Model to Improve Calculation of Shock Separated Flows

The Menter Shear Stress Transport (SST) k . turbulence model is one of the most widely used two-equation Reynolds-averaged Navier-Stokes turbulence models for aerodynamic analyses. The model extends Menter s baseline (BSL) model to include a limiter that prevents the calculated turbulent shear stress from exceeding a prescribed fraction of the turbulent kinetic energy via a proportionality constant, a1, set to 0.31. Compared to other turbulence models, the SST model yields superior predictions of mild adverse pressure gradient flows including those with small separations. In shock - boundary layer interaction regions, the SST model produces separations that are too large while the BSL model is on the other extreme, predicting separations that are too small. In this paper, changing a1 to a value near 0.355 is shown to significantly improve predictions of shock separated flows. Several cases are examined computationally and experimental data is also considered to justify raising the value of a1 used for shock separated flows.

Georgiadis, Nicholas J.↗
NASA NTRS1988

Resolved shear stress intensity coefficient and fatigue crack growth in large crystals

Fatigue crack growth tests were carried out on large-grain Al 7029 aluminum alloy and the finite element method was used to calculate the stress field near the tip of a zigzag crack. The resolved shear stresses on all 12 slip systems were computed, and the resolved shear stress intensity coefficient (RSSIC) was defined. The RSSIC was used to analyze the irregular crack path and was correlated with the rate of single-slip-plane shear crack growth. Fatigue crack growth was found to be caused primarily by shear decohesion at a crack tip. When the RSSIC on a single-slip system was much larger than all the others, the crack followed a single-slip plane. When the RSSICs on two conjugate slip systems were comparable, a crack grew in a zigzag manner on these planes and the macrocrack-plane bisected the two active slip planes. The maximum RSSIC on the most active slip system is proposed as a parameter to correlate with the shear fatigue crack growth rate in large crystals.

Chen, Q.↗
NASA NTRS1975

The measurement of shear stress and total heat flux in a nonadiabatic turbulent hypersonic boundary layer

Turbulent shear stress and direct turbulent total heat-flux measurements have been made across a nonadiabatic, zero pressure gradient, hypersonic boundary layer by using specially designed hot-wire probes free of strain-gauging and wire oscillation. Heat-flux measurements were in reasonably good agreement with values obtained by integrating the energy equation using measured profiles of velocity and temperature. The shear-stress values deduced from the measurements, by assuming zero correlation of velocity and pressure fluctuations, were lower than the values obtained by integrating the momentum equation. Statistical properties of the cross-correlations are similar to corresponding incompressible measurements at approximately the same momentum-thickness Reynolds number.

Mikulla, V.↗
NASA NTRS1931

Wrinkling of reinforced plates subjected to shear stresses

An analysis is made here of the problem of long plates with transverse stiffeners subject to shear. A typical example would be a long Wagner beam. The shear stress is calculated at which the web wrinkles and shear stress becomes a maximum. The equation is solved for both a condition of free support and rigidity of support on the edges.

Seydel, Edgar↗
NASA NTRS1999

Steady and transient fluid shear stress stimulate NO release in osteoblasts through distinct biochemical pathways

Fluid flow has been shown to be a potent stimulus in osteoblasts and osteocytes and may therefore play an important role in load-induced bone remodeling. The objective of this study was to investigate the characteristics of flow-activated pathways. Previously we reported that fluid flow stimulates rapid and continuous release of nitric oxide (NO) in primary rat calvarial osteoblasts. Here we demonstrate that flow-induced NO release is mediated by shear stress and that this response is distinctly biphasic. Transients in shear stress associated with the onset of flow stimulated a burst in NO production (8.2 nmol/mg of protein/h), while steady flow stimulated sustained NO production (2.2 nmol/mg of protein/h). Both G-protein inhibition and calcium chelation abolished the burst phase but had no effect on sustained production. Activation of G-proteins stimulated dose-dependent NO release in static cultures of both calvarial osteoblasts and UMR-106 osteoblast-like cells. Pertussis toxin had no effect on NO release. Calcium ionophore stimulated low levels of NO production within 15 minutes but had no effect on sustained production. Taken together, these data suggest that fluid shear stress stimulates NO release by two distinct pathways: a G-protein and calcium-dependent phase sensitive to flow transients, and a G-protein and calcium-independent pathway stimulated by sustained flow.

Non-NASA Center↗
NASA NTRS1986

An equilibrium method for prediction of transverse shear stresses in a thick laminated plate

First two equations of equilibrium are utilized to compute the transverse shear stress variation through thickness of a thick laminated plate after in-plane stresses have been computed using an assumed quadratic displacement triangular element based on transverse inextensibility and layerwise constant shear angle theory (LCST). Centroid of the triangle is the point of exceptional accuracy for transverse shear stresses. Numerical results indicate close agreement with elasticity theory. An interesting comparison between the present theory and that based on assumed stress hybrid finite element approach suggests that the latter does not satisfy the condition of free normal traction at the edge. Comparison with numerical results obtained by using constant shear angle theory suggests that LCST is close to the elasticity solution while the CST is closer to classical (CLT) solution. It is also demonstrated that the reduced integration gives faster convergence when the present theory is applied to a thin plate.

Chaudhuri, R. Z.↗
NASA NTRS1943

Effect of Normal Pressure on the Critical Shear Stress of Curved Sheet

In order to determine the critical stresses caused by an outward acting pressure on the upper surface of a wing due to the difference in internal and external pressures, torsional tests were made on two curved-sheet specimens subjected to an outward acting normal pressure. Results show that an outward acting normal pressure appreciable raises the critical shear stress for an unstiffened curved sheet; the absolute increase in critical shear stress is slightly greater for a 30 in. rib spacing than for a 10 in. rib spacing.

TESTING MACHINES, STRESS - NACA↗
NASA NTRS2002

Shear stress stimulates phosphorylation of eNOS at Ser(635) by a protein kinase A-dependent mechanism

Shear stress stimulates nitric oxide (NO) production by phosphorylating endothelial NO synthase (eNOS) at Ser(1179) in a phosphoinositide-3-kinase (PI3K)- and protein kinase A (PKA)-dependent manner. The eNOS has additional potential phosphorylation sites, including Ser(116), Thr(497), and Ser(635). Here, we studied these potential phosphorylation sites in response to shear, vascular endothelial growth factor (VEGF), and 8-bromocAMP (8-BRcAMP) in bovine aortic endothelial cells (BAEC). All three stimuli induced phosphorylation of eNOS at Ser(635), which was consistently slower than that at Ser(1179). Thr(497) was rapidly dephosphorylated by 8-BRcAMP but not by shear and VEGF. None of the stimuli phosphorylated Ser(116). Whereas shear-stimulated Ser(635) phosphorylation was not affected by phosphoinositide-3-kinase inhibitors wortmannin and LY-294002, it was blocked by either treating the cells with a PKA inhibitor H89 or infecting them with a recombinant adenovirus-expressing PKA inhibitor. These results suggest that shear stress stimulates eNOS by two different mechanisms: 1) PKA- and PI3K-dependent and 2) PKA-dependent but PI3K-independent pathways. Phosphorylation of Ser(635) may play an important role in chronic regulation of eNOS in response to mechanical and humoral stimuli.

Non-NASA Center↗
NASA NTRS1982

Wrinkling of Stretched Films: Shear Stress

Report presents theoretical investigation on nonlinear shearing characteristics of wrinkling films under applied shear stress. Report helps explain force/deflection characteristic of in-planeboom and solar-array blanket structural combinations.

Zak, M. A.↗
NASA NTRS1977

Representation of turbulent shear stress by a product of mean velocity differences

A quadratic form in the mean velocity for the turbulent shear stress is presented. It is expressed as the product of two velocity differences whose roots are the maximum velocity in the flow and a cutoff velocity below which the turbulent shear stress vanishes. Application to pipe and channel flows yields the centerline velocity as a function of pressure gradient, as well as the velocity profile. The flat plate, boundary-layer problem is solved by a system of integral equations to obtain friction coefficient, displacement thickness, and momentum-loss thickness. Comparisons are made with experiment.

Braun, W. H.↗