SEARCH · Engineering Papers

Results for “Microbiome”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

SourceResearch connectionFrom yearThrough year

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 73 records · Page 4

All sources NASA NTRS DOE OSTI

NASA NTRS2015

Research from the NASA Twins Study and Omics in Support of Mars Missions

The NASA Twins Study, NASA's first foray into integrated omic studies in humans, illustrates how an integrated omics approach can be brought to bear on the challenges to human health and performance on a Mars mission. The NASA Twins Study involves US Astronaut Scott Kelly and his identical twin brother, Mark Kelly, a retired US Astronaut. No other opportunity to study a twin pair for a prolonged period with one subject in space and one on the ground is available for the foreseeable future. A team of 10 principal investigators are conducting the Twins Study, examining a very broad range of biological functions including the genome, epigenome, transcriptome, proteome, metabolome, gut microbiome, immunological response to vaccinations, indicators of atherosclerosis, physiological fluid shifts, and cognition. A novel aspect of the study is the integrated study of molecular, physiological, cognitive, and microbiological properties. Major sample and data collection from both subjects for this study began approximately six months before Scott Kelly's one year mission on the ISS, continue while Scott Kelly is in flight and will conclude approximately six months after his return to Earth. Mark Kelly will remain on Earth during this study, in a lifestyle unconstrained by this study, thereby providing a measure of normal variation in the properties being studied. An overview of initial results and the future plans will be described as well as the technological and ethical issues raised for spaceflight studies involving omics.

Kundrot, C.↗

NASA NTRS2016

Effect of Low Shear Modeled Microgravity (LSMMG) on the Probiotic Lactobacillus Acidophilus ATCC 4356

The introduction of generally recognized as safe (GRAS) probiotic microbes into the spaceflight food system has the potential for use as a safe, non-invasive, daily countermeasure to crew microbiome and immune dysregulation. However, the microgravity effects on the stress tolerances and genetic expression of probiotic bacteria must be determined to confirm translation of strain benefits and to identify potential for optimization of growth, survival, and strain selection for spaceflight. The work presented here demonstrates the translation of characteristics of a GRAS probiotic bacteria to a microgravity analog environment. Lactobacillus acidophilus ATCC 4356 was grown in the low shear modeled microgravity (LSMMG) orientation and the control orientation in the rotating wall vessel (RWV) to determine the effect of LSMMG on the growth, survival through stress challenge, and gene expression of the strain. No differences were observed between the LSMMG and control grown L. acidophilus, suggesting that the strain will behave similarly in spaceflight and may be expected to confer Earth-based benefits.

Stahl, S.↗

NASA NTRS2016

Veggie: Space Vegetables for the International Space Station and Beyond

The Veggie vegetable production system was launched to the International Space Station (ISS) in 2014. Veggie was designed by ORBITEC to be a compact, low mass, low power vegetable production system for astronaut crews. Veggie consists of a light cap containing red, blue, and green LEDs, an extensible transparent bellows, and a baseplate with a root mat reservoir. Seeds are planted in plant pillows, small growing bags that interface with the reservoir. The Veggie technology validation test, VEG-01, was initiated with the first test crop of 'Outredgeous' red romaine lettuce. Prior to flight, lettuce seeds were sanitized and planted in a substrate of arcillite (baked ceramic) mixed with controlled release fertilizer. Upon initiation, astronauts open the packaged plant pillows, install them in the Veggie hardware, and prime the system with water. Operations include plant thinning, watering, and photography. Plants were grown on the ISS for 33 days, harvested, and returned frozen to Earth for analysis. Ground controls were conducted at Kennedy Space Center in controlled environment chambers reproducing ISS conditions of temperature, relative humidity, and CO2. Returned plant samples were analyzed for microbial food safety and chemistry including elements, antioxidants, anthocyanins and phenolics. In addition the entire plant microbiome was sequenced, and returned plant pillows were analyzed via x-ray tomography. Food safety analyses allowed us to gain approvals for future consumption of lettuce by the flight surgeons and the payload safety office. A second crop of lettuce was grown in 2015, and the crew consumed half the produce, with the remainder frozen for later analysis. This growth test was followed by testing of a new crop in Veggie, zinnias. Zinnias were grown to test a longer duration flowering crop in preparation for tests of tomatoes and other fruiting crops in the future. Zinnias were harvested in February. Samples from the second harvest of lettuce and the zinnia harvest are frozen on the ISS and will return with the next cargo return flight. Some challenges occurred in all tests, especially in the area of watering, with plants receiving insufficient or excess water leading to stressed growth. Zinnia plants were also challenged with fungal growth. Initial tests with Veggie have given us great insight into future crop production scenarios as we work to develop regular supplemental salad crop production on ISS and larger food production systems for our journey to Mars. Funding for this research comes from NASA's Space Biology Program.

Food Safety↗

NASA NTRS2016

Risk of Adverse Health Effects Due to Host-Microorganism Interactions

Numerous spaceflight experiments have been conducted to investigate alterations in microbial responses resulting from culture during spaceflight and spaceflight-analogs. However, recent studies investigating spaceflight-associated alterations in microbial virulence have initiated the review and production of evidence to better understand the impact these alterations would have on the incidence of infectious disease during a spaceflight exploration mission. The preponderance of evidence indicates that alterations in microbial gene expression and phenotype (including virulence) are occurring; however, the clinical implications of such changes are still unclear. Greater knowledge is required including a better understanding of the mechanism behind unique spaceflight-associated microbial responses to determine how this environmental stimulus impacts various microorganisms, their diversity and concentration in the spacecraft and crew microbiome, their impact on the vehicle and crew, and their resistance to current mitigation and antibiotic regimens. This knowledge will enable us to determine requirements, guidelines, and processes for design and monitoring of the next generation vehicles.

Ott, C. Mark↗

NASA NTRS2018

A Review and Comparison of Mouse and Rat Responses to Micro Gravity, Hyper Gravity and Simulated Models of Partial Gravity; Species Differences, Gaps in the Available Data, and Consideration of the Advantages and Caveats of Each Model for Spaceflight

Laboratory strains of mice and rat are widely used to study mammalian responses to stimulus, and both have been studied under a variety of gravity conditions, including space flight. We compared results obtained from exposure to spaceflight and microgravity, hyper gravity via centrifugation, earth gravity, and models of simulated partial gravity (hind-limb unloading and partial weight bearing treatments). We examined the reported changes in survival, body mass, circadian rhythm (body temperature and activity levels), behavior, bone, muscle, immune, cardio-vasculature, vestibular, reproduction and neonate survival, microbiome, and the visual system. Not all categories have published data for both species, some have limited data, and there are variations in experiment design that allow for only relative comparisons to be considered. The data reveal species differences in both the level of gravity required to obtain a response, degree of response, and in temporal expression of responses. Examination of the data across the gravity levels allows consideration of the hypothesis that gravitational responses follow a continuum, and organ specific differences are noted. In summary, we present advantages and caveats of each model system as pertains to gravitational biology research and identify gaps in our knowledge of how these mammals respond to gravity.

mouse rat gravity↗

NASA NTRS2018

Biology IS the Technology: the Microbial Ecology of Space Food Production and the Power of Aquaponics as a Learning Tool

To accomplish the objective of human missions to Mars and/or the long-term colonization of the moon, bioregenerative life support systems and food production systems will be absolutely necessary. Microbes are an essential and unavoidable component of these systems. In fact, these systems are driven by complex microbial communities about which we know very little, a glaring strategic knowledge gap in our ability to support extended human exploration in closed systems. Our laboratory has been working to use molecular ecological methods, including nanopore sequencing technology already deployed on the International Space Station, to understand the microbes in food production systems on Earth. Our ultimate goal is to inform the implementation of food production systems off-world. To date, we have sampled and sequenced the microbiomes of aquaponics systems, hydroponics systems, and fish ponds. Our results have revealed that the microbial communities in these systems are extremely diverse, and highly variable between systems. Along the way, we have discovered the power of aquaponics systems as teaching tools, and the capacity of students to perform high quality citizen science. By designing, constructing, and operating aquaponics systems, students better understand the role of microbes in the cycling of the elements in natural ecosystems, and in the human built environment. In partnership with schools and colleges, contributing new knowledge as citizen scientists, we are now exploring the relationships between the functioning of these systems and their microbial flora.

Bebout, Brad↗

NASA NTRS2018

Monitoring Astronaut Health with DNA Sequencing

In recent years microbe a plethora of microbe populations have been identified onboard the ISS (International Space Station). Approaches for real-time tracking of microbes for routine housekeeping and food/water safety monitoring will be critical for mission safety and crew health on future longer duration missions to the Moon or Mars. This work is a proof-of-concept study demonstrating an end-to-end phylogenetic identification and full genome sequencing effort of multiple microbial populations. Our methodology utilized the ISS flight-certified WetLab-2 molecular toolbox and the Biomolecule Sequencer projects for real-time end-to-end on-orbit microbial biological samples processing and molecular analysis with real time results generated utilizing only field "offline" analytic software. For this experiment we colony-cultured several ISS isolated microorganisms before generation of the pre-sequencing library via the automated VolTRAX device which enabled high library turnover with little wet-bench activity or potential future costly astronaut time. The pre-sequencing library is diluted in loading buffer and injected into the MinION sample port, drawn into the nanopore window by capillary action, and sequenced using the MinKnown. 16S and full genome alignment, nucleotide matching, gene identification, and phylogenetic sorting was accomplished utilizing the Epi2me software and the offline NCBI Blast viral, microbiome, and human somatic databases. In short, the methodologies developed herein replace the myriad of specific, often highly targeted microbiological tests used in the clinical laboratory, which would be difficult if not impossible to currently implement aboard the ISS or in deep space, with a single metagenomics test.

genomics↗

NASA NTRS2018

An Evolutionary Computation System Design Concept for Developing Controlled Closed Ecosystems: An Intelligent Systems Approach to Foster Gravitational Ecosystem Research for Developing Sustainable Communities in Space and on Earth

An adjustably-autonomous intelligent systems approach for developing Closed Ecosystems (CESs) is presented, which includes a design concept and preliminary design details for the Controlled Closed-Ecosystem Development System (CCEDS) and the Orbiting Modular Artificial-Gravity Spacecraft (OMAGS). The paper is divided into three sections: CESs, the CCEDS Design Concept, and Orbiting Fractional-Gravity Closed Ecosystems OMAGS design concept. The first section briefly describes Closed EcoSystems (CESs), complex adaptive systems, biomes, microbial microbiomes, and their relevance for the study of astrobiology. This section also discusses initial efforts in the development of Closed Environment Life Support Systems (CELSSs) for sustainable communities in space and on Earth. This section concludes with a discussion of the bioregenerative life support system challenge of and the corresponding consequences due to the inverse relationship of the very small human biomass/non-human biomass ratio overall on the Earth with respect to the extremely large human biomass/non-human-biomass ratio found in cities and the International Space Station. The second section describes the CCEDS design concept, which consists of a population of controlled colonies of CES Modules (CESMs), each an integrated CES, continually generating data for an intelligent system that operates the CESs and their CESMs. A variety of CESM types and their use are briefly described. The CCEDS intelligent system uses an evolutionary computation algorithm described in this section to develop and optimize these CESs to increase their viability duration and the size of the animals they support with the ultimate goal to support populations of humans, both on Earth and in space. The CCEDS architecture, its five control subsystems, and its five evolutionary computation levels are also discussed. The section concludes with a discussion of several CCEDS design strategies. The third section summarizes the OMAGS design concept for a spacecraft with a payload consisting of CESs in an orbiting spacecraft centrifuge that operates for at least 5 years. The spacecraft concept is described including its 150cm-radius centrifuge with a 2 ton & 3,000 liter bioscience payload capacity for 24 CESMs. The centrifuge design has four physical levels for its CESMs, each level subject to a different fractional gravity level. This section presents the spacecraft benefits of being designed and operated such that the spacecraft and payload centrifuge wheel counter-rotate resulting in net zero angular momentum and zero gyroscopic forces. Artificial-gravity generation by centripetal acceleration is also discussed. This section concludes by showing the external specifications of the CESMs and their layout in the centrifuge, followed by discussing the multi-payload module rationale. In tandem, the CCEDS and OMAGS systems can be used to foster gravitational ecosystem research for developing sustainable communities in space and on Earth.

Dorais, Gregory A.↗

NASA NTRS2019

Leafy Greens Grown on the International Space Station May Provide a Nutritious Supplement to Astronauts' Diet

Supplemental safe food production has been an essential goal of NASA to meet the nutritional needs of astronauts on the International Space Station (ISS) as well as for future long duration missions to the moon and beyond. Food crops grown in space experience different environmental conditions than plants grown on Earth (i.e. microgravity and spaceflight physical sciences impacts). To test the growth methods and effects of the space environment, red romaine lettuce Lactuca sativa cv. 'Outredgeous', was grown in Veggie plant growth chambers on the ISS. Microbiological food safety of the plants grown on the ISS was determined by heterotrophic plate counts to assess total microbial load for bacteria and fungi as well as screening for specific pathogens and isolate identification. Molecular characterization was completed using Next Generation Sequencing (NGS) to provide valuable information on the taxonomic composition and community structure of the plant microbiome. Chemical analyses of plant tissue were conducted to understand spaceflight-induced changes in key elements in the space diet, phenolics, anthocyanin levels, and Oxygen radical absorbance capacity (ORAC), a measure of antioxidant capacity. Three growth tests of red romaine lettuce were completed on ISS, VEG-01A, VEG-01B, and VEG-03A. Plants were harvested using two harvest methods, either a single terminal harvest (after 33 days) or cut-and-come-again repetitive harvesting (64 days total growth). Ground controls were grown simultaneously with a delay to accommodate condition monitoring and replication. A comparison of the plant tissue returned to Earth showed leaves from the second grow-out had significantly higher bacterial counts than the preceding or subsequent growth test or any of the ground controls. Fungal counts were significantly higher on the final cut-and-come-again harvest of the third grow out. None of the potential foodborne pathogens that were screened for were detected. Bacterial and fungal isolate identification and community characterization indicated similar diversity between VEG-01A and VEG-01B growth tests, however, there appeared to be subtle differences in diversity and distribution among the three growth tests. Chemical analysis of plant tissue revealed significant variation in a few elemental data, but variation in levels of phenolics, anthocyanins, and ORAC was not significantly different. This study indicated that leafy vegetable crops could safely provide an edible supplement to astronauts' diet, and our analysis provided baseline data for continual operation of the Veggie plant growth units on ISS. This research was funded by NASA's space biology program.

Food Production↗

NASA NTRS2019

Systemic Alterations with Spaceflight Associated Health Risks Originating from Both Circulating miRNAs and Mitochondrial Biology

The many known health risks currently associated with space travel include increased risk of cardiovascular disease, cancer, central nervous system related diseases, muscle degeneration, and changes with host-gut microbiome interactions that can have profound impact with these and other health risks. The majority of the risk from space travel stem of the two components of the space environment which are microgravity and radiation. Two specific systemic effects have been uncovered by us to impact the body as a whole due to the space environment. One factor is related from our earlier work (Beheshti et al, PLOS One, 2018), we predicted that there is a systemic component of the host that causes general increased health risks due to spaceflight driven by a circulating microRNA (miRNA) signature consisting of 13 miRNAs that directly regulates both p53 and TGF1. MiRNAs are small non-coding RNA molecules with a negative and post-transcriptional regulation on gene expression) are increasingly recognized as major systemic regulators of responses to stressors, including microgravity, oxidative stress, and DNA damage. In addition, due to the size and stability of miRNAs, it is known that miRNAs can circulate throughout the body and have been found in the majority of the bodily fluids including blood, urine, saliva, and tears. Here, we start to dissect the actual impact of this miRNA signature on both the radiation and microgravity components and prove that this miRNA signature actually exists in the circulation of a host. The other systemic factor we uncovered was the impact the mitochondria on the whole body due to spaceflight. We hypothesize that spaceflight may promote a physiologic response driven by systemic mitochondria pathways leading to metabolic disorder stemming from the liver and directly impacting other organs and tissues. A systems biology method was implemented utilizing GeneLab datasets that involved in vitro experiments performed at the low Earth orbit, in vivo experiments involving mice flown to space, and finally human physiological data from astronauts. A comprehensive multi-omics approach was implemented which involved correlating transcriptomic analysis with proteomics, metabolomics, and methylation analysis. This approach led us to confirm our hypothesis that a systemic mitochondrial driven response is responsible for increasing potential health risk and is conserved from the in vitro studies, to the in vivo studies, and finally confirmed in astronauts.

Radiation↗

NASA NTRS2019

Circulating miRNA Signature Predicts Health Risks Associated with Radiation and Microgravity

The many known health risks currently associated with space travel include increased risk of cardiovascular disease, cancer, central nervous system related diseases, muscle degeneration, and changes with host-gut microbiome interactions that can have profound impact with these and other health risks. The majority of the risk from space travel stem of the two components of the space environment which are microgravity and radiation. From our earlier work (Beheshti et al, PLOS One, 2018), we predicted that there is a systemic component of the host that causes general increased health risks due to spaceflight driven by a circulating microRNA (miRNA) signature consisting of 13 miRNAs that directly regulates both p53 and TGF1. MiRNAs are small non-coding RNA molecules with a negative and post-transcriptional regulation on gene expression) are increasingly recognized as major systemic regulators of responses to stressors, including microgravity, oxidative stress, and DNA damage. In addition, due to the size and stability of miRNAs, it is known that miRNAs can circulate throughout the body and have been found in the majority of the bodily fluids including blood, urine, saliva, and tears. Here, we start to dissect the actual impact of this miRNA signature on both the radiation and microgravity components and prove that this miRNA signature actually exists in the circulation of a host. To achieve this, we obtained multiple tissues including, serum, liver, and spleen and utilizing droplet digital PCR (ddPCR), we start to show how this circulating miRNA signature impacts which component of the spaceflight. The tissue was obtained from experiments performed on C57BL/6 male mice (N=10 for each condition) that were hindlimb unloaded (HU) to simulated microgravity, irradiated with 2Gy gamma (IR), HU plus IR, and control mice under normal conditions. It was shown that these miRNAs were present in the serum as predicted by the in silico prediction from our earlier predictions. The HU vs Controls show significant increases of the predicted miRNAs in the serum for more than half of the miRNA signature, with remaining miRNAs increasing comparing to the controls close to statistical significance. IR vs control mice showed increases for the miRNAs, but not has pronounced as the HU conditions. Finally, the combination of the HU+IR vs controls showed increases for the majority of the miRNA signature. The data indicates that the miRNA signature originally predicted through in silico methods is mainly associated with the microgravity component and is circulating throughout the host resulting in a systemic impact of the miRNAs on the host. These miRNAs are shown in the literature to potentially increase health risks associated with several diseases. In addition, we have begun testing the potential of utilizing antagonists to this miRNA signature to act as a potential countermeasure to mitigate radiation impact on the organism. This work demonstrates for the first time the potential of a minimally invasive novel biomarker and countermeasure that can be used to mitigate both radiation and microgravity effects.

Beheshti, Afshin↗

NASA NTRS2019

Nasa GeneLab Computomics Reveal Horizontal Gene Transfer on International Space Station Environmental Metagenomes

Prokaryotic lifeforms can be observed to demonstrate many keen adaptive advantages, perhaps facilitated by a nature simplistic relative to divergent domains of life. In particular, decompartmentalized gene expression facilitates adaptation by allowing free exchange of genetic material, albeit at the cost of increased susceptibility to genetic damage. Thus, these lifeforms must compensate by embracing diverse investment strategies in an attempt to “brute force” the evolvability equation through precipitous genesis, lean metabolic efficiency, and sheer population. This prokaryotic archetype also enables symbiotic relationships with secondary mobile genetic elements known as plasmids, which have been shown to drive evolution on rapid temporal scales through processes such as conjugation and transformation. This study attempts to decipher whether these mechanisms of horizontal gene transfer (HGT) are major factors in determining prokaryote fitness within a unique isolated environment, the International Space Station (ISS). The ISS Microbial Tracking (MT) project has generated a wealth of data concerning the successive reigns of microbial genera that appear to thrive amidst harsh conditions for life. Despite relatively higher doses of ionizing radiation as compared to Earth, complications associated with microgravity, and the anti-microbial mélange deployed, microbial life still persists in this environment. The NASA GeneLab serves as a data repository and analysis platform to enable researchers to access space flight factor related data. With the use of GeneLab’s modern computational suites (computomics), phylogenetic and functional genomic investigations of HGT events were conducted on the data generated from the MT-1 project. The putative data concerning the plasmid population (plasmidome) of the ISS was algorithmically derived and compared to those of habitats with similar environmental dynamics- such as living quarters and hospitals- to investigate whether these HGT elements may play crucial role(s) in shaping the microbiome of this closed habitat that serves as the only inhabited structure in space.

Bense, Nicholas↗

NASA NTRSDate not supplied

Bioregenerative Life Support for Humans in Space

To date, human life support in space has been achieved through physico-chemical methods along with stowage and resupply. But biological approaches could be used as well, such as growing plants (crops) to produce oxygen and food, while removing carbon dioxide generated by the humans. The plants and their associated microbiome could also be used to help recycle wastewater. These so-called bioregenerative approaches for human life support become more favorable as mission durations and distances increase. For example, a baseline mission to Mars would require about 3 years, including transit time to and from Mars, and living on the surface. For these and longer durations missions, life support approaches will need to be highly closed and sustainable, with minimal mass and power requirements. Based on testing by NASA and other space agencies around the world, about 20-25 m2 of crops could provide the oxygen needs for one human, while about 50 m2 of crops could provide the food(dietary calories) for one person. But this is dependent on lighting provided to the crops and optimizing the agricultural practices. In many ways, these goals for space agriculture have similar challenges to sustainable agriculture and living on Earth. The talk will review some of NASA’s research on biological life support and its potential for future space exploration.

Life Support↗

NASA NTRS2021

Adding Fresh Produce to the Space Diet

NASA’s Human Research Program has identified a Risk of inadequate nutrition and food stability as a risk of high concern for future long duration exploration missions such as a mission to Mars. One possible solution to help reduce this risk is the addition of in situ produced supplemental fresh produce to supplement and enhance the astronaut diet. A number of ground and ISS-based studies are being conducted which are helping to reduce this risk by filling the gaps in knowledge and technology associated with space crop production. These include studies with the Veggie vegetable production system on the International Space Station. Key focus areas include water and nutrient delivery, food safety and the crop microbiome, crop selection and testing, automation and robotics, radiation impacts, seed storage and handling, and scalability for different concepts and architectures. All of these areas have significant challenges that need to be addressed prior to successful integration of crop production into the crew diet. Data from these studies is feeding the development of new technologies and systems for space crop production. This research was funded by NASA Space Biology and NASA’s Human Research Program.

Gioia Massa↗

NASA NTRSDate not supplied

Testing of the Veggie Vegetable Production System on the International Space Station

The Veggie vegetable production system has been flying on the International Space Station since 2014, with a second Veggie chamber added in 2017. Veggie is a simple, low power, flexible platform for space plant cultivation. Since 2014, over a dozen crop cycles have been conducted in Veggie. Primarily leafy green crops have been grown, and many of these have been used to supplement the crew diet. Flowers were also grown, and the seeds returned produced viable progeny. In addition to the crop plants, Veggie has been used as a platform for model plants grown in Petri dishes or custom magenta jar chambers. Other used have included algal culture and an education seed germination experiment. Science in Veggie has focused on the plant microbiome, the chemistry and food safety of space-grown produce, the impacts of light quality on crop growth, and the behavioral health benefits of cultivating and eating space crops. Veggie is allowing testing and maturation of NASA’s early-stage space crop production research in a relevant environment, and the lessons that we are learning from this research are helping to shape future exploration scenarios. This research was funded by NASA Space Biology and NASA’s Human Research Program.

Veggie↗

NASA NTRSDate not supplied

Adding Fresh Produce to the Space Diet

Space Crop Production↗

NASA NTRSDate not supplied

Testing of the Veggie Vegetable Production System on the International Space Station

Veggie↗

NASA NTRSDate not supplied

Space Crop Production

As astronauts venture farther from Earth, and for longer periods, food will become increasingly critical. Crop production can supplement a packaged diet to provide additional nutrients and variety for astronauts. Testing with the Veggie and Advanced Plant Habitat chambers on the International Space Station is allowing us to understand the impacts of gravity and spaceflight on crop growth and nutritional content, and the importance of plants to astronauts living and working away from our home planet. The space environment presents unique challenges to crop production, and technology and knowledge gaps have been identified. Key gaps include how to: - Identify and demonstrate effective options to provide both water and oxygen to the root zone in microgravity - Understand the deep space radiation impacts on seeds and plants - Investigate the relationship between microbiomes and food safety - Store and handle seeds to ensure they are viable, free of contaminants and long-lived - Identify / develop potential crops suitable for the space environment - Understand automation and human factors - Determine scalability for different concepts and architectures Filling these gaps will help enable future human exploration and move us toward Earth-independence.

Space Crop Production↗