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59 records · Page 4

Genetic analysis of the roles of phytochromes A and B1 in the reversed gravitropic response of the lz-2 tomato mutant

The lz-2 mutation in tomato (Lycopersicon esculentum) causes conditional reversal of shoot gravitropism by light. This response is mediated by phytochrome. To further elicit the mechanism by which phytochrome regulates the lz-2 phenotype, phytochrome-deficient lz-2 plants were generated. Introduction of au alleles, which severely block chromophore biosynthesis, eliminated the reversal of hypocotyl gravitropism in continuous red and far-red light. The fri1 and tri1 alleles were introduced to specifically deplete phytochromes A and B1, respectively. In dark-grown seedlings, phytochrome A was necessary for response to high-irradiance far-red light, a complete response to low fluence red light, and also mediated the effects of blue light in a far-red reversible manner. Loss of phytochrome B1 alone did not significantly affect the behaviour of lz-2 plants under any light treatment tested. However, dark-grown lz-2 plants lacking both phytochrome A and B1 exhibited reduced responses to continuous red and were less responsive to low fluence red light and high fluence blue light than plants that were deficient for phytochrome A alone. In high light, full spectrum greenhouse conditions, lz-2 plants grew downward regardless of the phytochrome deficiency. These results indicate that phytochromes A and B1 play significant roles in mediating the lz-2 phenotype and that at least one additional phytochrome is involved in reversing shoot gravitropism in this mutant.

NASA Discipline Plant Biology↗

Rotor dynamic behaviour of a high-speed oil-free motor compressor with a rigid coupling supported on four radial magnetic bearings

The combination of a high-speed motor, dry gas seals, and magnetic bearings realized in this unit facilitates the elimination of oil. The motor is coupled with a quill shaft to the compressor. This yields higher natural frequencies of the rotor than with the use of a diaphragm coupling and helps to maintain a sufficient margin of the maximum speed to the frequency of the second compressor bending mode. However, the controller of each bearing then has to take the combined modes of both machines into account. The requirements for the controller to ensure stability and sufficient damping of all critical speeds are designed and compared with the implemented controller. The calculated closed loop behavior was confirmed experimentally, except the stability of some higher modes due to slight frequency deviations of the rotor model to the actual rotor. The influence of a mechanical damper as a device to provide additional damping to high models is demonstrated theoretically. After all, it was not necessary to install the damper, since all modes cold be stabilized by the controller.

Schmied, J.↗

The Magnetar Nature and the Outburst Mechanism of a Transient Anomalous X-ray Pulsar

Anomalous X-ray Pulsars (AXPs) belong to a class of neutron stars believed to harbor the strongest magnetic fields in the universe, as indicated by their energetic bursts and their rapid spindowns. However, a direct measurement of their surface field strengths has not been made to date. It is also not known whether AXP outbursts result from changes in the neutron star magnetic field or crust properties. Here we report the first, spectroscopic measurement of the surface magnetic field strength of an AXP, XTE J1810-197, and solidify its magnetar nature. The field strength obtained from detailed spectral analysis and modeling is remarkably close to the value inferred from the rate of spindown of this source and remains nearly constant during numerous observations spanning over two orders of magnitude in source flux. The surface temperature, on the other hand, declines steadily and dramatically following the 2003 outburst of this source. Our findings demonstrate that heating occurs in the upper neutron star crust during an outburst and sheds light on the transient behaviour of AXPs.

Guver, Tolga↗

Root cytoskeleton: its role in perception of and response to gravity

We have critically evaluated the possible functions of the plant cytoskeleton in root gravisensing and graviresponse and discussed the evidence that microtubules (MTs) and actin microfilaments (MFs) do not control differential cell growth during bending of roots. On the other hand, MF and MT networks are envisaged to participate in gravisensing because of the mechanical properties of the cytoskeletal structures that interconnect plant cell organelles with the plasma membrane. In restrained gravisensing, forces are suggested to be transmitted to membranes because large-scale gravity-dependent repositioning of organelles is effectively prevented due to the cytoskeleton-mediated anchorage of their envelopes at the plasma membrane. From the cytoskeletal point of view, we can also envisage an unrestrained gravity sensing when cytoskeletal tethers are not strong enough to preserve the tight control over distribution of organelles and the latter, if heavy enough, are allowed to sediment towards the physical bottom of cells. This situation obviously occurs in root cap statocytes because these uniquely organized cells are depleted of prominent actin MF bundles, endoplasmic MT arrays, and ER elements in their internal cytoplasm. Nevertheless, indirect evidence clearly indicates that sedimented root cap statoliths are enmeshed within fine but dynamic MF networks and that their behaviour is obviously under, at least partial, cytoskeletal control. The actomyosin-enriched domain among and around amyloplasts is proposed to increase the perception of gravity due to the grouping effect of sedimenting statoliths. Cytoskeletal links between myosin-rich statoliths, and cell peripheries well equipped with dense cortical MTs, membrane-associated cytoskeleton, as well as with ER elements, would allow efficient restrained gravisensing only at the statocyte cell cortex. As a consequence of cytoskeletal depletion in the internal statocyte cytoplasm and bulk sedimentation of large amyloplasts, restrained gravisensing is spatially restricted to the bottom of the statocyte irrespective of whether roots are vertical or horizontal. This spatial aspect allows for efficient gravisensing via amplification of gravity-induced impacts on the cellular architecture, a phenomenon which is unique to root cap statocytes.

NASA Discipline Plant Biology↗

The Central Engine of GRB 130831A and the Energy Breakdown of a Relativistic Explosion

Gamma-ray bursts (GRBs) are the most luminous explosions in the Universe, yet the nature and physical properties of their energy sources are far from understood. Very important clues, however, can be inferred by studying the afterglows of these events. We present optical and X-ray observations of GRB 130831A obtained by Swift, Chandra, Skynet, Reionization And Transients Infra-Red camera, Maidanak, International Scientific Optical-Observation Network, Nordic Optical Telescope, Liverpool Telescope and Gran Telescopio Canarias. This burst shows a steep drop in the X-ray light curve at asymptotically equal to 10(exp 5) s after the trigger, with a power-law decay index of alpha that is approximately 6. Such a rare behaviour cannot be explained by the standard forward shock (FS) model and indicates that the emission, up to the fast decay at 10(exp 5) s, must be of internal origin, produced by a dissipation process within an ultrarelativistic outflow. We propose that the source of such an outflow, which must produce the X-ray flux for an asymptotically equal to 1 d in the cosmological rest frame, is a newly born magnetar or black hole. After the drop, the faint X-ray afterglow continues with a much shallower decay. The optical emission, on the other hand, shows no break across the X-ray steep decrease, and the late-time decays of both the X-ray and optical are consistent. Using both the X-ray and optical data, we show that the emission after an asymptotically equal to 10(exp 5) scan be explained well by the FS model. We model our data to derive the kinetic energy of the ejecta and thus measure the efficiency of the central engine of a GRB with emission of internal origin visible for a long time. Furthermore, we break down the energy budget of this GRB into the prompt emission, the late internal dissipation, the kinetic energy of the relativistic ejecta,and compare it with the energy of the associated supernova, SN 2013 fu.

radiation mechanisms: non-thermal – shock waves ↗