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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 73 records · Page 4
All sourcesNASA NTRSDOE OSTI
DOE OSTI2019

A Revised Architectural Survey of the Nuclear Engine Maintenance Assembly and Disassembly Facility, Area 25, Nevada National Security Site, Nye County, Nevada

The U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) anticipates closure activities under the Federal Facility Agreement Consent Order (FFACO) and eventual demolition of the Engine Maintenance and Disassembly (E-MAD) facility, to meet Environmental Management mission requirements. The NNSA/NFO has obligations under Section 106 of the National Historic Preservation Act (NHPA) and its implementing regulations (36 C.F.R. Part 800) to consider the effects of its actions and undertakings on historically important properties and to develop and implement strategies to minimize or mitigate adverse effects to these resources. This Section 106 architectural survey and evaluation of the buildings and structures in the E-MAD facility was done under requirements of 36 C.F.R § 800.4 - Identification of historic properties. Desert Research Institute (DRI) completed an architectural survey of a 21-acre area of potential effect in February 2019. DRI previously surveyed the entire E-MAD facility as a single architectural resource eligible for inclusion in the National Register of Historic Places (NRHP or National Register) under Criteria A and C and Consideration G and as a contributor to the potential Nuclear Rocket Development Station (NRDS) Historic District (Beck et al. 1996).

54 ENVIRONMENTAL SCIENCES↗
DOE OSTI2024

Universal correlation for falling film evaporation heat transfer coefficients of water and seawater

Horizontal falling film evaporators are widely utilized in desalination industries to increase fresh water supply. However, universal correlations for seawater falling film evaporation under varied operational and geometrical conditions are simply unavailable in open literature. Thus, this study aims to develop such a universal correlation for both plain and enhanced tubes. The detailed heat transfer mechanisms are reviewed, and rational parameters are incorporated to develop the universal correlation. A dataset of 994 data points from 9 sources covering a wide range of conditions was compiled. These conditions include Reynolds numbers from 10 to 7235, heat fluxes from 7.7 to 208 kW/m -2 , saturation temperatures from 278 to 401 K, salinities from 0 to 60 gsalt kg -1 water, tube diameters from 15.8 to 50.8 mm, and liquid feeder height to diameter ratios from 1 to 2.25. Upon analysis, it was found that most of the recommended existing correlations exhibited poor predictive accuracy, as evidenced by larger MADs. The developed correlation in this study demonstrated the best predictive accuracy for the entire dataset, yielding a MAD of 16.8 % and an R 2 of 0.82. Furthermore, the performance of the new correlation was individually assessed across a broader spectrum of operational and design conditions, reflecting the individual conditions’ influences with an overall MAD of 20 %.

42 ENGINEERING↗
DOE OSTI2014

A novel low energy electron microscope for DNA sequencing and surface analysis

Monochromatic, aberration-corrected, dual-beam low energy electron microscopy (MAD-LEEM) is a novel technique that is directed towards imaging nanostructures and surfaces with sub-nanometer resolution. The technique combines a monochromator, a mirror aberration corrector, an energy filter, and dual beam illumination in a single instrument. The monochromator reduces the energy spread of the illuminating electron beam, which significantly improves spectroscopic and spatial resolution. Simulation results predict that the novel aberration corrector design will eliminate the second rank chromatic and third and fifth order spherical aberrations, thereby improving the resolution into the sub-nanometer regime at landing energies as low as one hundred electron-Volts. The energy filter produces a beam that can extract detailed information about the chemical composition and local electronic states of non-periodic objects such as nanoparticles, interfaces, defects, and macromolecules. The dual flood illumination eliminates charging effects that are generated when a conventional LEEM is used to image insulating specimens. A potential application for MAD-LEEM is in DNA sequencing, which requires high resolution to distinguish the individual bases and high speed to reduce the cost. The MAD-LEEM approach images the DNA with low electron impact energies, which provides nucleobase contrast mechanisms without organometallic labels. Furthermore, the micron-size field of view when combined with imaging on the fly provides long read lengths, thereby reducing the demand on assembling the sequence. Finally, experimental results from bulk specimens with immobilized single-base oligonucleotides demonstrate that base specific contrast is available with reflected, photo-emitted, and Auger electrons. Image contrast simulations of model rectangular features mimicking the individual nucleotides in a DNA strand have been developed to translate measurements of contrast on bulk DNA to the detectability of individual DNA bases in a sequence.

47 OTHER INSTRUMENTATION↗
DOE OSTI2025

Orthorhombic Structures as Inorganic Halide Perovskite Models for High-Throughput DFT Investigations

Although high-symmetry Pm-3m space group cubic models are computationally efficient for high-throughput density functional theory (DFT) calculations of inorganic ternary (ABX 3 ) halide perovskites (HPs), they frequently predict band gaps (E g ) that disagree with experiment. Conversely, while low-symmetry cubic polymorphous networks (PN) comprised of 160 to 320 atoms incur significantly greater computational cost, they predict E g ’s that are more closely aligned with experiment. Here, in this study, we compare the DFT total energies and E g ’s predicted by four high-symmetry structure models (Pnma orthorhombic, R3m trigonal, P4/mbm tetragonal, and Pm-3m cubic) to cubic PNs for 5 experimentally characterized ternary HPs and find that the orthorhombic model computes Eg’s with the smallest MAD of 0.23 eV relative to the PNs. Pair distribution functions and DFT-computed total energies show that octahedral tilting, which is present in the 20-atom orthorhombic and 160-atom cubic PN models but not in the 5-atom cubic models, stabilizes all 5 compositions in our benchmarking set. We also find that imposing PN constraints when generating and optimizing these orthorhombic structures by fixing the unit cell lattice vectors and displacing the atoms prior to ionic relaxation with DFT, which we call the orthorhombic surrogate model (OSM), lowers the MAD of E g predictions to 0.09 eV. Our OSM predicts the PN band gaps of an additional 95 theoretical inorganic ternary HPs with MAD of 0.08 eV, supporting its usage in high-throughput DFT investigations to closely estimate PN band gaps with much less computational expense.

14 SOLAR ENERGY↗
DOE OSTI2014

Atomic layer epitaxy of Ruddlesden-Popper SrO(SrTiO{sub 3}){sub n} films by means of metalorganic aerosol deposition

We report an atomic layer epitaxial growth of Ruddlesden-Popper (RP) thin films of SrO(SrTiO{sub 3}){sub n} (n = ∞, 2, 3, 4) by means of metalorganic aerosol deposition (MAD). The films are grown on SrTiO{sub 3}(001) substrates by means of a sequential deposition of Sr-O/Ti-O{sub 2} atomic monolayers, monitored in-situ by optical ellipsometry. X-ray diffraction and transmission electron microscopy (TEM) reveal the RP structure with n = 2–4 in accordance with the growth recipe. RP defects, observed by TEM in a good correlation with the in-situ ellipsometry, mainly result from the excess of SrO. Being maximal at the film/substrate interface, the SrO excess rapidly decreases and saturates after 5–6 repetitions of the SrO(SrTiO{sub 3}){sub 4} block at the level of 2.4%. This identifies the SrTiO{sub 3} substrate surface as a source of RP defects under oxidizing conditions within MAD. Advantages and limitations of MAD as a solution-based and vacuum-free chemical deposition route were discussed in comparison with molecular beam epitaxy.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗
DOE OSTI2016

Performance of quantum Monte Carlo for calculating molecular bond lengths

This work investigates the accuracy of real-space quantum Monte Carlo (QMC) methods for calculating molecular geometries. We present the equilibrium bond lengths of a test set of 30 diatomic molecules calculated using variational Monte Carlo (VMC) and diffusion Monte Carlo (DMC) methods. The effect of different trial wavefunctions is investigated using single determinants constructed from Hartree-Fock (HF) and Density Functional Theory (DFT) orbitals with LDA, PBE, and B3LYP functionals, as well as small multi-configurational self-consistent field (MCSCF) multi-determinant expansions. When compared to experimental geometries, all DMC methods exhibit smaller mean-absolute deviations (MADs) than those given by HF, DFT, and MCSCF. The most accurate MAD of 3 ± 2 × 10{sup −3} Å is achieved using DMC with a small multi-determinant expansion. However, the more computationally efficient multi-determinant VMC method has a similar MAD of only 4.0 ± 0.9 × 10{sup −3} Å, suggesting that QMC forces calculated from the relatively simple VMC algorithm may often be sufficient for accurate molecular geometries.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗
DOE OSTI2012

Coordinate-Space Hartree-Fock-Bogoliubov Solvers for Super fluid Fermi Systems in Large Boxes

The self-consistent Hartree-Fock-Bogoliubov problem in large boxes can be solved accurately in the coordinate space with the recently developed solvers HFB-AX (2D) and MADNESS-HFB (3D). This is essential for the description of superfluid Fermi systems with complicated topologies and significant spatial extend, such as fissioning nuclei, weakly-bound nuclei, nuclear matter in the neutron star rust, and ultracold Fermi atoms in elongated traps. The HFB-AX solver based on B-spline techniques uses a hybrid MPI and OpenMP programming model for parallel computation for distributed parallel computation, within a node multi-threaded LAPACK and BLAS libraries are used to further enable parallel calculations of large eigensystems. The MADNESS-HFB solver uses a novel multi-resolution analysis based adaptive pseudo-spectral techniques to enable fully parallel 3D calculations of very large systems. In this work we present benchmark results for HFB-AX and MADNESS-HFB on ultracold trapped fermions.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2015

SU-E-J-158: Audiovisual Biofeedback Reduces Image Artefacts in 4DCT: A Digital Phantom Study

Purpose: Irregular breathing motion has a deleterious impact on 4DCT image quality. The breathing guidance system: audiovisual biofeedback (AVB) is designed to improve breathing regularity, however, its impact on 4DCT image quality has yet to be quantified. The purpose of this study was to quantify the impact of AVB on thoracic 4DCT image quality by utilizing the digital eXtended Cardiac Torso (XCAT) phantom driven by lung tumor motion patterns. Methods: 2D tumor motion obtained from 4 lung cancer patients under two breathing conditions (i) without breathing guidance (free breathing), and (ii) with guidance (AVB). There were two breathing sessions, yielding 8 tumor motion traces. This tumor motion was synchronized with the XCAT phantom to simulate 4DCT acquisitions under two acquisition modes: (1) cine mode, and (2) prospective respiratory-gated mode. Motion regularity was quantified by the root mean square error (RMSE) of displacement. The number of artefacts was visually assessed for each 4DCT and summed up for each breathing condition. Inter-session anatomic reproducibility was quantified by the mean absolute difference (MAD) between the Session 1 4DCT and Session 2 4DCT. Results: AVB improved tumor motion regularity by 30%. In cine mode, the number of artefacts was reduced from 61 in free breathing to 40 with AVB, in addition to AVB reducing the MAD by 34%. In gated mode, the number of artefacts was reduced from 63 in free breathing to 51 with AVB, in addition to AVB reducing the MAD by 23%. Conclusion: This was the first study to compare the impact of breathing guidance on 4DCT image quality compared to free breathing, with AVB reducing the amount of artefacts present in 4DCT images in addition to improving inter-session anatomic reproducibility. Results thus far suggest that breathing guidance interventions could have implications for improving radiotherapy treatment planning and interfraction reproducibility.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

SU-G-JeP3-05: Geometry Based Transperineal Ultrasound Probe Positioning for Image Guided Radiotherapy

Purpose: The use of ultrasound (US) imaging in radiotherapy is not widespread, primarily due to the need for skilled operators performing the scans. Automation of probe positioning has the potential to remove this need and minimize operator dependence. We introduce an algorithm for obtaining a US probe position that allows good anatomical structure visualization based on clinical requirements. The first application is on 4D transperineal US images of prostate cancer patients. Methods: The algorithm calculates the probe position and orientation using anatomical information provided by a reference CT scan, always available in radiotherapy workflows. As initial test, we apply the algorithm on a CIRS pelvic US phantom to obtain a set of possible probe positions. Subsequently, five of these positions are randomly chosen and used to acquire actual US volumes of the phantom. Visual inspection of these volumes reveal if the whole prostate, and adjacent edges of bladder and rectum are fully visualized, as clinically required. In addition, structure positions on the acquired US volumes are compared to predictions of the algorithm. Results: All acquired volumes fulfill the clinical requirements as specified in the previous section. Preliminary quantitative evaluation was performed on thirty consecutive slices of two volumes, on which the structures are easily recognizable. The mean absolute distances (MAD) between actual anatomical structure positions and positions predicted by the algorithm were calculated. This resulted in MAD of 2.4±0.4 mm for prostate, 3.2±0.9 mm for bladder and 3.3±1.3 mm for rectum. Conclusion: Visual inspection and quantitative evaluation show that the algorithm is able to propose probe positions that fulfill all clinical requirements. The obtained MAD is on average 2.9 mm. However, during evaluation we assumed no errors in structure segmentation and probe positioning. In future steps, accurate estimation of these errors will allow for better evaluation of the achieved accuracy.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2016

TU-AB-202-12: A Novel Method to Map Endoscopic Video to CT for Treatment Planning and Toxicity Analysis in Radiation Therapy

Purpose: Endoscopic examinations are routine procedures for head-and-neck cancer patients. Our goal is to develop a method to map the recorded video to CT, providing valuable information for radiotherapy treatment planning and toxicity analysis. Methods: We map video frames to CT via virtual endoscopic images rendered at the real endoscope’s CT-space coordinates. We developed two complementary methods to find these coordinates by maximizing real-to-virtual image similarity:(1)Endoscope Tracking: moves the virtual endoscope frame-by-frame until the desired frame is reached. Utilizes prior knowledge of endoscope coordinates, but sensitive to local optima. (2)Location Search: moves the virtual endoscope along possible paths through the volume to find the desired frame. More robust, but more computationally expensive. We tested these methods on clay phantoms with embedded markers for point mapping and protruding bolus material for contour mapping, and we assessed them qualitatively on three patient exams. For mapped points we calculated 3D-distance errors, and for mapped contours we calculated mean absolute distances (MAD) from CT contours. Results: In phantoms, Endoscope Tracking had average point error=0.66±0.50cm and average bolus MAD=0.74±0.37cm for the first 80% of each video. After that the virtual endoscope got lost, increasing these values to 4.73±1.69cm and 4.06±0.30cm. Location Search had point error=0.49±0.44cm and MAD=0.53±0.28cm. Point errors were larger where the endoscope viewed the surface at shallow angles<10 degrees (1.38±0.62cm and 1.22±0.69cm for Endoscope Tracking and Location Search, respectively). In patients, Endoscope Tracking did not make it past the nasal cavity. However, Location Search found coordinates near the correct location for 70% of test frames. Its performance was best near the epiglottis and in the nasal cavity. Conclusion: Location Search is a robust and accurate technique to map endoscopic video to CT. Endoscope Tracking is sensitive to erratic camera motion and local optima, but could be used in conjunction with anchor points found using Location Search.

60 APPLIED LIFE SCIENCES↗
DOE OSTI2024

Collapsar Gamma-Ray Bursts Grind Their Black Hole Spins to a Halt

Abstract The spin of a newly formed black hole (BH) at the center of a massive star evolves from its natal value due to two competing processes: accretion of gas angular momentum that increases the spin and extraction of BH angular momentum by outflows that decreases the spin. Ultimately, the final, equilibrium spin is set by a balance between both processes. In order for the BH to launch relativistic jets and power aγ-ray burst (GRB), the BH magnetic field needs to be dynamically important. Thus, we consider the case of a magnetically arrested disk (MAD) driving the spin evolution of the BH. By applying the semianalytic MAD BH spin evolution model of Lowell et al. to collapsars, we show that if the BH accretes ∼20% of its initial mass, its dimensionless spin inevitably reaches small values,a≲ 0.2. For such spins, and for mass accretion rates inferred from collapsar simulations, we show that our semianalytic model reproduces the energetics of typical GRB jets,L jet ∼ 10 50 erg s −1 . We show that our semianalytic model reproduces the nearly constant power of typical GRB jets. If the MAD onset is delayed, this allows powerful jets at the high end of the GRB luminosity distribution,L jet ∼ 10 52 erg s −1 , but the final spin remains low,a≲ 0.3. These results are consistent with the low spins inferred from gravitational wave detections of binary BH mergers. In a companion paper by Gottlieb et al., we use GRB observations to constrain the natal BH spin to bea≃ 0.2.

Astronomy & Astrophysics↗
DOE OSTI2024

Jets with a Twist: The Emergence of FR0 Jets in a 3D GRMHD Simulation of Zero-angular-momentum Black Hole Accretion

Abstract Spinning supermassive black holes (BHs) in active galactic nuclei magnetically launch relativistic collimated outflows, or jets. Without angular momentum supply, such jets are thought to perish within 3 orders of magnitude in distance from the BH, well before reaching kiloparsec scales. We study the survival of such jets at the largest scale separation to date, via 3D general relativistic magnetohydrodynamic simulations of rapidly spinning BHs immersed into uniform zero-angular-momentum gas threaded by a weak vertical magnetic field. We place the gas outside the BH sphere of influence, or the Bondi radius, chosen to be much larger than the BH gravitational radius, R B = 10 3 R g . The BH develops dynamically important large-scale magnetic fields, forms a magnetically arrested disk (MAD), and launches relativistic jets that propagate well outside R B and suppress BH accretion to 1.5% of the Bondi rate, M ̇ B . Thus, low-angular-momentum accretion in the MAD state can form large-scale jets in Fanaroff–Riley (FR) type I and II galaxies. Subsequently, the disk shrinks and exits the MAD state: barely a disk (BAD), it rapidly precesses, whips the jets around, globally destroys them, and lets 5%–10% of M ̇ B reach the BH. Thereafter, the disk starts rocking back and forth by angles 90°–180°: the rocking accretion disk (RAD) launches weak intermittent jets that spread their energy over a large area and suppress BH accretion to ≲2% M ̇ B . Because the BAD and RAD states tangle up the jets and destroy them well inside R B , they are promising candidates for the more abundant, but less luminous, class of FR0 galaxies.

Astronomy & Astrophysics↗
DOE OSTI2023

Large-scale Evolution of Seconds-long Relativistic Jets from Black Hole–Neutron Star Mergers

We present the first numerical simulations that track the evolution of a black hole–neutron star (BH–NS) merger from premerger to r ≳ 10 11 cm. The disk that forms after a merger of mass ratio q = 2 ejects massive disk winds (3–5 × 10 –2 M ⊙ ). We introduce various postmerger magnetic configurations and find that initial poloidal fields lead to jet launching shortly after the merger. The jet maintains a constant power due to the constancy of the large-scale BH magnetic flux until the disk becomes magnetically arrested (MAD), where the jet power falls off as L j ~ t –2 . All jets inevitably exhibit either excessive luminosity due to rapid MAD activation when the accretion rate is high or excessive duration due to delayed MAD activation compared to typical short gamma-ray bursts (sGRBs). This provides a natural explanation for long sGRBs such as GRB 211211A but also raises a fundamental challenge to our understanding of jet formation in binary mergers. One possible implication is the necessity of higher binary mass ratios or moderate BH spins to launch typical sGRB jets. For postmerger disks with a toroidal magnetic field, dynamo processes delay jet launching such that the jets break out of the disk winds after several seconds. We show for the first time that sGRB jets with initial magnetization σ0 > 100 retain significant magnetization (σ $\gg$ 1) at r > 10 10 cm, emphasizing the importance of magnetic processes in the prompt emission. The jet–wind interaction leads to a power-law angular energy distribution by inflating an energetic cocoon whose emission is studied in a companion paper.

79 ASTRONOMY AND ASTROPHYSICS↗
DOE OSTI2021

Characterizing the performance of a POPS miniaturized optical particle counter when operated on a quadcopter drone

We first validate the performance of the Portable Optical Particle Spectrometer (POPS), a small light-weight and high sensitivity optical particle counter, against a reference scanning mobility particle sizer (SMPS) for a month-long deployment in an environment dominated by biomass burning aerosols. Subsequently, we examine any biases introduced by operating the POPS on a quadcopter drone, a DJI Matrice 200 V2. We report the root mean square difference (RMSD) and mean absolute difference (MAD) in particle number concentrations (PNCs) when mounted on the UAV and operating on the ground and when hovering at 10 m. When wind speeds are low (less than 2.6 m s –1 ), we find only modest differences in the RMSDs and MADs of 5 % and 3 % when operating at 10 m altitude. When wind speeds are between 2.6 and 7.7 m s –1 the RMSDs and MADs increase to 26.2 % and 19.1 %, respectively, when operating at 10m altitude. No statistical difference in PNCs was detected when operating on the UAV in either ascent or descent. We also find size distributions of aerosols in the accumulation mode (defined by diameter, d, where 0.1 ≤ d ≤ 1 µm) are relatively consistent between measurements at the surface and measurements at 10 m altitude, while differences in the coarse mode (here defined by d > 1 µm) are universally larger. Our results suggest that the impact of the UAV rotors on the POPS PNCs are small at low wind speeds, but when operating under a higher wind speed of up to 7.6 m s –1 , larger discrepancies occur. In addition, it appears that the POPS measures sub-micron aerosol particles more accurately than super-micron aerosol particles when airborne on the UAV. These measurements lay the foundations for determining the magnitude of potential errors that might be introduced into measured aerosol particle size distributions and concentrations owing to the turbulence created by the rotors on the UAV.

47 OTHER INSTRUMENTATION↗
NASA NTRS1981

Mean thermal and albedo behavior of the Mars surface and atmosphere over a Martian year

A Mars average data set (MADS) has been constructed from thermal and albedo measurements of the Viking Infrared Thermal Mapper; by merging information from all longitudes, and ensuring reasonably complete longitudinal sampling, a representation of mean Mars behavior is obtained. Brightness temperatures at 7, 9, 11, 15, and 20 microns and albedo information in the band 0.3-3.0 microns have been binned using 2 deg latitude strips, 24 times of day, 3 emission angle intervals, and 23 nonoverlapping solar longitude periods covering 1.43 Mars years starting at a solar longitude of 84 deg. The MADS is ideally suited to parametric study of latitudinal, diurnal, angular, and seasonal dependences. Data are presented for surface thermal and albedo behavior in clear and dusty atmospheric conditions; the thermal response of the atmospheric temperature to a major dust storm is found to be consistent with Mariner 9 data from the 1971 storm. Examples of use of the MADS, which is available through the Mars Consortium, indicate how averaged data reveal specific surface and atmospheric phenomena.

Martin, T. Z.↗
NASA NTRS1986

Independent Orbiter Assessment (IOA): Analysis of the instrumentation subsystem

The results of the Independent Orbiter Assessment (IOA) of the Failure Modes and Effects Analysis (FMEA) and Critical Items List (CIL) are presented. The IOA approach features a top-down analysis of the hardware to determine failure modes, criticality, and potential critical items. To preserve independence, this analysis was accomplished without reliance upon the results contained within the NASA FMEA/CIL documentation. The independent analysis results for the Instrumentation Subsystem are documented. The Instrumentation Subsystem (SS) consists of transducers, signal conditioning equipment, pulse code modulation (PCM) encoding equipment, tape recorders, frequency division multiplexers, and timing equipment. For this analysis, the SS is broken into two major groupings: Operational Instrumentation (OI) equipment and Modular Auxiliary Data System (MADS) equipment. The OI equipment is required to acquire, condition, scale, digitize, interleave/multiplex, format, and distribute operational Orbiter and payload data and voice for display, recording, telemetry, and checkout. It also must provide accurate timing for time critical functions for crew and payload specialist use. The MADS provides additional instrumentation to measure and record selected pressure, temperature, strain, vibration, and event data for post-flight playback and analysis. MADS data is used to assess vehicle responses to the flight environment and to permit correlation of such data from flight to flight. The IOA analysis utilized available SS hardware drawings and schematics for identifying hardware assemblies and components and their interfaces. Criticality for each item was assigned on the basis of the worst-case effect of the failure modes identified.

Howard, B. S.↗
NASA NTRS2001

Application of multivariate statistics to vestibular testing: discriminating between Meniere's disease and migraine associated dizziness

Meniere's disease (MD) and migraine associated dizziness (MAD) are two disorders that can have similar symptomatologies, but differ vastly in treatment. Vestibular testing is sometimes used to help differentiate between these disorders, but the inefficiency of a human interpreter analyzing a multitude of variables independently decreases its utility. Our hypothesis was that we could objectively discriminate between patients with MD and those with MAD using select variables from the vestibular test battery. Sinusoidal harmonic acceleration test variables were reduced to three vestibulo-ocular reflex physiologic parameters: gain, time constant, and asymmetry. A combination of these parameters plus a measurement of reduced vestibular response from caloric testing allowed us to achieve a joint classification rate of 91%, independent quadratic classification algorithm. Data from posturography were not useful for this type of differentiation. Overall, our classification function can be used as an unbiased assistant to discriminate between MD and MAD and gave us insight into the pathophysiologic differences between the two disorders.

NASA Discipline Neuroscience↗
NASA NTRS2000

Cardiac tissue enriched factors serum response factor and GATA-4 are mutual coregulators

Combinatorial interaction among cardiac tissue-restricted enriched transcription factors may facilitate the expression of cardiac tissue-restricted genes. Here we show that the MADS box factor serum response factor (SRF) cooperates with the zinc finger protein GATA-4 to synergistically activate numerous myogenic and nonmyogenic serum response element (SRE)-dependent promoters in CV1 fibroblasts. In the absence of GATA binding sites, synergistic activation depends on binding of SRF to the proximal CArG box sequence in the cardiac and skeletal alpha-actin promoter. GATA-4's C-terminal activation domain is obligatory for synergistic coactivation with SRF, and its N-terminal domain and first zinc finger are inhibitory. SRF and GATA-4 physically associate both in vivo and in vitro through their MADS box and the second zinc finger domains as determined by protein A pullout assays and by in vivo one-hybrid transfection assays using Gal4 fusion proteins. Other cardiovascular tissue-restricted GATA factors, such as GATA-5 and GATA-6, were equivalent to GATA-4 in coactivating SRE-dependent targets. Thus, interaction between the MADS box and C4 zinc finger proteins, a novel regulatory paradigm, mediates activation of SRF-dependent gene expression.

NASA Discipline Musculoskeletal↗