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A METHOD TO REDUCE BIOBURDEN IN ASTROMATERIALS CURATION FACILITIES WITHOUT INTRODUCING UNWANTED CONTAMINATION

Introduction: NASA curates its Astromaterials collections in cleanrooms that are carefully monitored for particulate, inorganic and trace metal contamination. Current sample collections are not particularly susceptible to organic contamination or biological alteration. However, new collections like those from the OSIRIS-REx and Hayabusa2 missions will have organic contamination requirements and are susceptible to biodegradation. It will be necessary sterilize or at least disinfect curation labs, as well as tools and equipment in a manner that does not introduce additional contamination and does not affect the samples 1. Current curation cleaning procedures utilize isopropyl alcohol which offers some bioburden reduction, but is not effective against spore-forming bacteria or fungal spores 2. We present a modified disinfection method that uses ultrapure hydrogen peroxide to reduce bioburden inside curation labs and glove boxes without introducing contamination or damaging curation equipment. We tested this method in the meteorite processing lab as well as on a glovebox being cleaned for use in processing ANGSA (Apollo Next Generation Sample Analysis) samples and present the results of those tests. We discuss the limitations of this method and describe potential situations in which it will not be applicable. The CDC guidelines for disinfection andsterilization in healthcare facilities discusses over 15different methods for reducing bioburden in hospitalsettings 3. The most common method, steamsterilization, is well suited to sterilizing curationprocessing tools but cannot easily be used to sterilizecleanroom surfaces or large equipment likegloveboxes. Chemical sterilization with bleach(NaOCl) is also a common strategy in healthcare andpharmaceutical settings that presents materialcompatibility issues as well as serious inorganiccontamination concerns for curation facilities.Introducing a new source of Na and Cl into curationlabs is not acceptable. Other chemical methods likeethylene oxide, formaldehyde, iodophors andquaternary ammonium compounds could introduceorganic and inorganic contamination. We chose tofocus on hydrogen peroxide because it is generallycompatible with commonly used curation materialslike stainless steel, aluminum and Teflon and becauseit decomposes to oxygen and water. The CDCguidelines for hydrogen peroxide specify using a 7.5wt% solution at 25 ̊C with a contact time of 30 minutesfor high level disinfection and 6 hours for sterilization.High level disinfection is defined as a technique thatwill kill all microorganisms except large numbers ofbacterial spores 3. Methods: We prepared a solution of 7.5 wt%hydrogen peroxide from a stock solution of ultrapure30 wt% peroxide (JT Baker) and curation gradeultrapure water. This ultrapure water is already used incuration cleaning procedures and thus is not consideredand additional source of contamination. We conducteda materials compatibility test by exposing unanodizedand anodized 6061 T6 Al alloy to the peroxide solutionfor up to six hours and periodically inspecting thesurfaces for visible defects. We used this peroxide todisinfect the floor of the meteorite processing lab andthe interior of a curation glovebox by exposing thesesurfaces to the peroxide solution for 30 min. Thesurfaces were swabbed with a dry macrofoam swabbefore (Puritan Brand 2518051PFRNDFD) and afterperoxide treatment to collect microbes present on thesurfaces. Microbes were extracted by sonication fromthe swab into 15 ml of PBS (phosphate buffered saline)and inoculated onto the following media: TSA (trypticsoy agar) BA (blood agar), R2A (Reasoners 2 agar),Potato Dextrose Agar, Saboraud Dextrose Agar andSaboraud Dextrose Agar with 0.1 mg/ mlchloramphenicol. Four TSA plates and two BA plateswere inoculated with 0.1 ml of PBS each andincubated at 35 and 37 for 48 hours. Two R2A°C°Cplates (0.1 ml of PBS each) were incubated at 25 .°CThe remaining plates were inoculated with 0.2ml ofPBS and incubated at 30 ̊C for seven days. Afterincubation bacterial and fungal isolates were countedand transferred to new plates for identification usingthe VITEK24 automated system or by sequencing aportion of the barcode gene (16S rRNA for bacteria,small subunit gene for fungi) on an ABI 3500 Sangersequencer. Negative controls consisted of swabs thatwere opened in the sampling environment andanalyzed alongside the experimental samples.Results: A 6 hour exposure to hydrogen peroxideresulted in visible pitting on un-anodized 6061 Al, butnot on anodized surfaces. No visible pitting occurredafter a 30 minute exposure. Therefore, we decided tolimit our experimental tests to 30 min. exposures. 17bacterial CFU (colony forming units) representing 4distinct organisms were isolated from the meteorite processing lab floor prior to hydrogen peroxidetreatment. We were unable culture any organisms afterperoxide treatment. In the glovebox we were able toculture three bacterial CFU representing three distinctspecies, including a spore forming bacterium prior todisinfection with peroxide. After the peroxidetreatment we were unable to culture any organisms.Routine monitoring of the meteorite processing lab andthe glovebox did not indicate any increase in unwantedinorganic contamination after these peroxidetreatments. Discussion: A 30 minute treatment with 7.5 wt%peroxide appears to be an effective method forreducing bioburden on typical cleanroom surfaces. Themethod does not introduce unwanted organic orinorganic contamination and is compatible withcommonly used curation materials like stainless steel,Teflon and anodized aluminum alloys. Special careshould be taken with un-anodized aluminum.Prolonged exposure to hydrogen peroxide can causepitting on this material. We recommend using thismethod to disinfect curation labs and equipment whenbiological alteration is a concern. This method iseffective at room temperature and cannot be used todisinfect labs and equipment where the ambienttemperature is < 0 ̊C. Astromaterials samples shouldbe removed from the area where disinfection is tooccur. Hydrogen peroxide is a powerful oxidizingagent and will react with any organic carbon present inthe sample. References: [1.] Mccubbin, F. M. et al.Sp. Sci Rev(2019) doi:10.1007/s11214-019-0615-9. [2.] Mogul, R.et al.Astrobiology 18, ast.2017.1814 (2018). [3.]Rutala, W. A. & Weber, D. J. Guideline for Disinfection and Sterilization in Healthcare Facilities, 2008. [4.] Pincus, D. H. in Encyclopedia of Rapid Microbiological Methods (2005).

A. B. Regberg↗

Technical Facilities Management, Loan Pool, and Calibration

My work at JPL for the SURF program began on June 11, 2012 with the Technical Facilities Management group (TFM). As well as TFM, I worked with Loan Pool and Metrology to help them out with various tasks. Unlike a lot of other interns, I did not have a specific project rather many different tasks to be completed over the course of the 10 weeks.The first task to be completed was to sort through old certification reports in 6 different boxes to locate reports that needed to be archived into a digital database. There were no reports within these boxes that needed to be archived but rather were to be shredded. The reports went back to the early 1980's and up to the early 2000's. I was looking for reports dated from 2002 to 2012

Technical Facilities Management group (TFM)↗

Resolution of Maldi-Tof Compared to Whole Genome Sequencing for Identification of Bacillus Species Isolated From Cleanrooms at Nasa Johnson Space Center

The Astromaterials Acquisition and Curation Office at NASA Johnson Space Center maintains cleanrooms to archive extraterrestrial materials returned from space exploration missions. Compared to typical built environments, oligotrophic conditions make these facilities inhospitable to microbes. Despite these controls, bacteria and fungi are regularly cultured from these cleanrooms. In particular, Bacillus sp. are frequently isolated during routine microbial monitoring. Endospores associated with this genus can survive extreme environments, such as cleanrooms. This microbial contamination may affect the integrity of astromaterials.

Microbiology↗

Technology Development and Advanced Planning for Curation of Returned Mars Samples

NASA Johnson Space Center (JSC) curates extraterrestrial samples, providing the international science community with lunar rock and soil returned by the Apollo astronauts, meteorites collected in Antarctica, cosmic dust collected in the stratosphere, and hardware exposed to the space environment. Curation comprises initial characterization of new samples, preparation and allocation of samples for research, and clean, secure long-term storage. The foundations of this effort are the specialized cleanrooms (class 10 to 10,000) for each of the four types of materials, the supporting facilities, and the people, many of whom have been doing detailed work in clean environments for decades. JSC is also preparing to curate the next generation of extraterrestrial samples. These include samples collected from the solar wind, a comet, and an asteroid. Early planning and R\&D are underway to support post-mission sample handling and curation of samples returned from Mars. One of the strong scientific reasons for returning samples from Mars is to search for evidence of current or past life in the samples. Because of the remote possibility that the samples may contain life forms that are hazardous to the terrestrial biosphere, the National Research Council has recommended that all samples returned from Mars be kept under strict biological containment until tests show that they can safely be released to other laboratories. It is possible that Mars samples may contain only scarce or subtle traces of life or prebiotic chemistry that could readily be overwhelmed by terrestrial contamination . Thus, the facilities used to contain, process, and analyze samples from Mars must have a combination of high-level biocontainment and organic / inorganic chemical cleanliness that is unprecedented. JSC has been conducting feasibility studies and developing designs for a sample receiving facility that would offer biocontainment at least the equivalent of current maximum containment BSL-4 (BioSafety Level 4) laboratories, while simultaneously maintaining cleanliness levels equaling those of state-of-the-art cleanrooms. Unique requirements for the processing of Mars samples have inspired a program to develop handling techniques that are much more precise and reliable than the approach (currently used for lunar samples) of employing gloved human hands in nitrogen-filled gloveboxes. Individual samples from Mars are expected to be much smaller than lunar samples, the total mass of samples returned by each mission being 0.5- 1 kg, compared with many tens of kg of lunar samples returned by each of the six Apollo missions. Smaller samples require much more of the processing to be done under microscopic observation. In addition, the requirements for cleanliness and high-level containment would be difficult to satisfy while using traditional gloveboxes. JSC has constructed a laboratory to test concepts and technologies important to future sample curation. The Advanced Curation Laboratory includes a new-generation glovebox equipped with a robotic arm to evaluate the usability of robotic and teleoperated systems to perform curatorial tasks. The laboratory also contains equipment for precision cleaning and the measurement of trace organic contamination.

Lindstrom, David J.↗

Wallops Flight Facility Integration and Testing Customer Handbook

The Wallops Integration and Testing Handbook is a guide for planning operations at Wallops testing facilities. It provides a summary of the capabilities and technical specifications of available facilities. This handbook prescribes the information to be provided by the user that will enable the integration and testing team to effectively plan for and support the facility user’s project. Note that each facility plans for availability based on current workloads/ mission schedules. For example, sounding rockets may have between 20 and 35 missions in work at one time. Also note that special training, such as cleanroom certification, electrostatic discharge (ESD) or confined space training may be required for entrance to some facilities.

Adam Hammoudi↗

A recent Cleanroom success story: The Redwing project

Redwing is the largest completed Cleanroom software engineering project in IBM, both in terms of lines of code and project staffing. The product provides a decision-support facility that utilizes artificial intelligence (AI) technology for predicting and preventing complex operating problems in an MVS environment. The project used the Cleanroom process for development and realized a defect rate of 2.6 errors/KLOC, measured from first execution. This represents the total amount of errors that were found in testing and installation at three field test sites. Development productivity was 486 LOC/PM, which included all development labor expended in design specification through completion of incremental testing. In short, the Redwing team produced a complex systems software product with an extraordinarily low error rate, while maintaining high productivity. All of this was accomplished by a project team using Cleanroom for the first time. An 'introductory implementation' of Cleanroom was defined and used on Redwing. This paper describes the quality and productivity results, the Redwing project, and how Cleanroom was implemented.

Hausler, Philip A.↗

Low-Pressure Generator Makes Cleanrooms Cleaner

Scientists at NASA's Kennedy Space Center work in cleanrooms: laboratories with high degrees of cleanliness provided by strict control of particles such as dust, lint, or human skin. They are contaminant-free facilities, where the air is repeatedly filtered, and surfaces are smooth to prevent particles from getting lodged. Technicians working in these environments wear specially designed cleanroom "bunny suits" and booties over their street clothes, as well as gloves and face masks to avoid any contamination that may be imparted from the outside world. Even normal paper is not allowed in cleanrooms, only cleanroom low-particulate paper. These are sensitive environments where precision work, like the production of silicon chips or hard disk drives, is performed. Often in cleanrooms, positive air pressure is used to force particles outside of the isolated area. The air pressure in the Kennedy cleanrooms is monitored using high-accuracy, low-differential pressure transducers that require periodic calibration. Calibration of the transducers is a tricky business. In prior years, the analysis was performed by sending the transducers to the Kennedy Standards Laboratory, where a very expensive cross-floated, labor- intensive, dead-weight test was conducted. In the early 1990s, scientists at Kennedy determined to develop a technique and find equipment to perform qualification testing on new low-differential pressure transducers in an accurate, cost-effective manner onsite, without requiring an environmentally controlled room. They decided to use the highly accurate, cost-effective Setra Model C264 as the test transducer. For qualification testing of the Setra, though, a portable, lower-cost calibrator was needed that could control the differential pressure to a high degree of resolution and transfer the accuracy of the Standards Laboratory testing to the qualification testing. The researchers decided that, to generate the low-differential pressure setpoints needed for qualification testing, very small gas volume changes could be made against the test article, and a corresponding pressure change would be detected by a pressure standard. This allowed the researchers to recreate cleanroom air pressure settings without the use of a cleanroom. Thus was born the low-differential pressure generator. In 1993, a prototype was developed using a pair of PVC tanks, a volume controller, and a 1-pound-per-square-inch pressure standard. By 1995, the prototype was perfected into the unit that is still used today.

Source record↗

Mars Sample Return (MSR) Sample Receiving Facility (SRF) Assessment Study (MSAS)

NASA, in partnership with the European Space Agency (ESA), is seeking to return Martian geological and atmospheric samples to Earth for scientific study in the early 2030s. Due to the possibility that the samples could contain extraterrestrial life, Mars Sample Return (MSR) is classified as a Category V: Restricted Earth Return mission by the NASA Planetary Protection Office. As a result of this classification, a MSR Sample Receiving Facility (SRF) must not only provide a pristine environment to ensure samples are protected from terrestrial contamination for scientific investigations, it must also provide high-containment (biosafety level 4 [BSL-4]-equivalence) to isolate the samples from Earth’s biosphere until the samples are deemed safe for release and/or sterilized. The nominal utilization period for a SRF is anticipated to be 2-5 years and is intended to enable curation activities, biohazard assessment, select early science activities, and the rapid release of samples to the scientific community. However, to account for possible delays in schedule or the identification of extant life, this anticipated period of time must be flexible to accommodate schedule extensions and contingency plans. Due to requirements for high-level biological containment and cleanliness, a traditional receiving/curation facility cannot be utilized for MSR. Therefore, beginning in 2022, NASA Johnson Space Center is performing a MSR SRF Assessment Study (MSAS) to investigate the most optimal facility modality for a MSR SRF, as well as start to define programmatic early estimate of costs and schedules before the initial design phase begins. NASA is partnering with industry contractors (architectural and engineering firms with BSL-4 and cleanroom technology experience, as well as other contracted infrastructure and construction specialists) along with selected experts from NASA, ESA, existing U.S. BSL-4 facilities, and other U.S. government agencies, to carry out the assessment study. The MSAS should also aid in the future refinement of the science requirements (e.g., contamination control, equipment accommodations) before site-specific design would commence. As part of the MSAS, NASA is planning to assess an array of possibilities for a MSR SRF. One of the main considerations is the facility modality and whether an existing BSL-4 facility can be utilized (for some or all functions); or, if new constructure would be required, would a traditional fixed facility or a modular facility the best choice. MSAS will also investigate the ability of the modalities to accommodate two different facility capability endmembers: 1) a minimal facility focusing on biohazard assessment and curation tasks with a small footprint, and 2) an enhanced facility with additional capabilities to enable expedited processing and the completion of time-sensitive and (some) sterilization-sensitive science. The assessment is intended to generate information that will inform the selection of facility modalities for high-level conceptual design development. While the assessment study will focus on SRF requirements for accommodating curation, science, and sample safety assessment infrastructure, it will also consider an array of other factors, such as ease of access for international users, decommissioning, repurposing, future sale or lease following MSR’s use of the facility, and uncontained preparatory laboratory spaces. Upon completion of the study, the preferred modality and refined requirements would be utilized for site-specific design but will not be finalized until NASA’s completion of the National Environmental Policy Act (NEPA) process.

A.D. Harrington↗

Genesis Solar Wind Sample Curation Facility

The controlled access facility designed and constructed for storing and examining Genesis solar wind samples consists of two adjacent laboratories, both ISO Class 4 cleanrooms (vertical laminar flow, ULPA filtered). One cleanroom is equipped with ultrapure water (UPW) for experimental cleaning of containers and tools used in handling the solar wind samples, flight hardware and witness plates. The ultrapure water is also used for cleaning the solar wind samples. The other cleanroom is for long-term nitrogen storage of samples and for examination and processing of Genesis samples.

Curation Facility↗

Evaluating the performance of portable air filter walls for the James Webb Space Telescope launch campaign

The James Webb Space Telescope (JWST) launched from the Centre Spatial Guyanais (CSG) in December 2021. JWST’s requirements permitted processing in International Organization for Standardization (ISO) Class 7 or better facilities, but CSG was only equipped with ISO Class 8 facilities. To temporarily upgrade the air cleanliness in the Payload Processing Facility, Fueling Hall, and Final Assembly Building Encapsulation Hall, NASA provided two portable High Efficiency Particulate Air (HEPA) and carbon filter walls that were used in each location. The walls were comprised of stacks of two modules high and arranged in push-push configurations as shown to be most effective via Computational Fluid Dynamics simulations of expected floor layouts in each facility. After delivery to NASA’s Goddard Space Flight Center in 2020, the walls underwent initial verification measurements inside a cleanroom and validation testing in an uncontrolled area to quantify their improvement of air cleanliness and particle and molecular fallout. Validation testing showed improvements of 83-99% for airborne particle counts, 79-91% for particle fallout, and 50-90% for molecular fallout. The particle improvements were applied to the contamination budget analysis that tracked current and predicted future cleanliness against End-of-Life requirements for JWST’s critical surfaces. At CSG, the walls successfully maintained an ISO Class 7 environment or better within their envelope in each location, despite their presence in ISO Class 8 facilities with dense integration operations.

HEPA, Contamination Control, Filtration↗

Evaluating the Performance of Portable Air Filter Walls for the James Webb Space Telescope Launch Campaign

The James Webb Space Telescope (JWST) launched from the Centre Spatial Guyanais (CSG) in December 2021. JWST’s requirements permitted processing in International Organization for Standardization (ISO) Class 7 or better facilities, but CSG was only equipped with ISO Class 8 facilities. To temporarily upgrade the air cleanliness in the Payload Processing Facility, Fueling Hall, and Final Assembly Building Encapsulation Hall, NASA provided two portable High Efficiency Particulate Air (HEPA) and carbon filter walls that were used in each location. The walls were comprised of stacks of two modules high and arranged in push-push configurations as shown to be most effective via Computational Fluid Dynamics simulations of expected floor layouts in each facility. After delivery to NASA’s Goddard Space Flight Center in 2020, the walls underwent initial verification measurements inside a cleanroom and validation testing in an uncontrolled area to quantify their improvement of air cleanliness and particle and molecular fallout. Validation testing showed improvements of 83-99% for airborne particle counts, 79-91% for particle fallout, and 50-90% for molecular fallout. The particle improvements were applied to the contamination budget analysis that tracked current and predicted future cleanliness against End-of-Life requirements for JWST’s critical surfaces. At CSG, the walls successfully maintained an ISO Class 7 environment or better within their envelope in each location, despite their presence in ISO Class 8 facilities with dense integration operations.

HEPA, Contamination Control, Filtration↗

Evaluation of SNOLAB background mitigation procedures through the use of an ICP-MS based dust monitoring methodology

Dust particulate fallout on materials in use for rare-event searches is a concerning source of radioactive backgrounds due to the presence of the naturally occurring radionuclides 40 K, 232 Th, 238 U, and their progeny in dust. Much effort is dedicated to inform radioactive backgrounds from dust and evaluate the efficacy of mitigation procedures. A great portion of such effort relies on fallout models and assumed dust composition. Here, in this work, an ICP-MS based methodology was employed for a direct determination of fallout rates of radionuclides and stable isotopes of interest from dust particulate at the SNOLAB facility. Hosted in an active mine, the SNOLAB underground laboratory strives to maintain experimental areas at class 2000 cleanroom level. This work validates the mitigation procedures in place at SNOLAB and informs dust backgrounds during laboratory activities. Fallout rates of major constituents of the local rock were measured two to three orders of magnitude lower in the clean experimental areas compared to non-clean transition areas from the mine to the laboratory. An increase of approximately two orders of magnitude in the fallout rate of stable Pb isotopes was determined in an experimental area during activities involving handling of Pb bricks. Increased 40 K, 232 Th, and 238 U fallout rates were measured in clean experimental areas during activities generating particulate.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Ames Engineering Directorate

The Ames Engineering Directorate is the principal engineering organization supporting aerospace systems and spaceflight projects at NASA's Ames Research Center in California's Silicon Valley. The Directorate supports all phases of engineering and project management for flight and mission projects-from R&D to Close-out-by leveraging the capabilities of multiple divisions and facilities.The Mission Design Center (MDC) has full end-to-end mission design capability with sophisticated analysis and simulation tools in a collaborative concurrent design environment. Services include concept maturity level (CML) maturation, spacecraft design and trades, scientific instruments selection, feasibility assessments, and proposal support and partnerships. The Engineering Systems Division provides robust project management support as well as systems engineering, mechanical and electrical analysis and design, technical authority and project integration support to a variety of programs and projects across NASA centers. The Applied Manufacturing Division turns abstract ideas into tangible hardware for aeronautics, spaceflight and science applications, specializing in fabrication methods and management of complex fabrication projects. The Engineering Evaluation Lab (EEL) provides full satellite or payload environmental testing services including vibration, temperature, humidity, immersion, pressure/altitude, vacuum, high G centrifuge, shock impact testing and the Flight Processing Center (FPC), which includes cleanrooms, bonded stores and flight preparation resources. The Multi-Mission Operations Center (MMOC) is composed of the facilities, networks, IT equipment, software and support services needed by flight projects to effectively and efficiently perform all mission functions, including planning, scheduling, command, telemetry processing and science analysis.

Small Satellites↗

Cleanroom Garment Silicone Contamination

The slide presentation reviews actions taken at Goddard Space Flight Center (GSFC) to eliminate contamination by silicone in clean rooms. Background information includes facilities and hardware affected by silicon contamination, a discussion of the negative aspects of silicone contamination, clean room garments, and how the problem was identified at GSFC. Actions taken by the GSFC Contamination Engineering Group and lessons learned are detailed. Results include: awareness of the silicone issue in laundry, increase in infrastructure and support of the testing lab, establishment of protocols for garment verification, closer relationship established with laundry and converter, specifications for laundry services and garments were strengthened, all consumables are tested before use in clean rooms, and established procedures were used to identify and treat silicone found on face masks.

Geer, Wayne↗

The New LOTIS Test Facility

The Large Optical Test and Integration Site (LOTIS) at the Lockheed Martin Space Systems Company in Sunnyvale, CA is designed for the verification and testing of optical systems. The facility consists of an 88 foot temperature stabilized vacuum chamber that also functions as a class 10k vertical flow cleanroom. Many problems were encountered in the design and construction phases. The industry capability to build large chambers is very weak. Through many delays and extra engineering efforts, the final product is very good. With 11 Thermal Conditioning Units and precision RTD s, temperature is uniform and stable within 1oF, providing an ideal environment for precision optical testing. Within this chamber and atop an advanced micro-g vibration-isolation bench is the 6.5 meter diameter LOTIS Collimator and Scene Generator, LOTIS alignment and support equipment. The optical payloads are also placed on the vibration bench in the chamber for testing. This optical system is designed to operate in both air and vacuum, providing test imagery in an adaptable suite of visible/near infrared (VNIR) and midwave infrared (MWIR) point sources, and combined bandwidth visible-through-MWIR point sources, for testing of large aperture optical payloads. The heart of the system is the LOTIS Collimator, a 6.5m f/15 telescope, which projects scenes with wavefront errors <85 nm rms out to a 0.75 mrad field of view (FOV). Using field lenses, performance can be extended to a maximum field of view of 3.2 mrad. The LOTIS Collimator incorporates an extensive integrated wavefront sensing and control system to verify the performance of the system.

Bell, R. M.↗

Flight Systems Integration & Test: Lessons Learned for Future Success

This paper offers a comprehensive view of flight system integration and test (I&T) lessons learned related to mishaps and close calls, focusing on what can be done to improve the I&T process to avoid recurrence. Specific areas within the realm of I&T that are covered in this paper include: I&T team communication and training; design of flight and ground systems for I&T; planning and scheduling; configuration management and process documentation; ground support equipment and tools; cleanrooms and contamination; mechanical integration, handling, and deployments; electrical integration and electrostatic discharge; functional testing and troubleshooting; environmental testing and facilities; and launch site operations. To illustrate "real-world" lessons learned for I&T, examples from throughout the history of the U.S. space program are presented. Also presented are some best practices for I&T that can help mitigate mishaps and close calls on the ground or during flight.

Integration & Test↗

Transforming any Facility for Meeting Strict Cleanliness Requirements

Over the life of the James Webb Space Telescope (JWST), Integration & Test (I&T) has taken place in areas that needed considerable work to make the facility itself and/or the protocols used while working in the rooms suitable to meet JWST percent area coverage (PAC) and molecular accumulation requirements. In addition to normal particulate matter, JWST had a uniquely significant challenge: fibers! Fibers not only cause much higher PAC levels, but they also risk damaging the angstrom sized Near Infrared Spectrometer (NIRSpec) microshutter array (MSA), which is critical to NIRSpec instrument performance. The primary emphasis of this paper is to address particulate and fiber contamination. The success of the JWST mission required effective cleanrooms, protocols, and mitigations in non-cleanroom areas that were pressed into service to house contamination-sensitive optics and scientific instruments. Some presented profound challenges. These included: NASA’s 60-year-old Johnson Space Center (JSC) Chamber A, which had never been used for anything contamination-sensitive, and the European tropical launch facilities, which were designed to meet International Standard Organization (ISO) Class 8 processing for communication satellites. The final challenge for JWST, as if to stare us in the face and say, “I dare you to try and make me clean enough,” was preparing the 4 areas in the Centre Spatial Guyanais (CSG) Final Assembly Building (BAF) located in French Guiana, a building in which one entire side opens for Ariane 5 rocket ingress and egress. This paper will describe our initial evaluation processes and the actual work undertaken to transform even the most challenging areas into first class cleanrooms that met JWST particulate and fiber requirements.

James Webb Space Telescope↗

Environmental Testing of Contamination Sensitive Instruments and Optics in Non-Cleanliness Controlled Facilities

The James Webb Space Telescope (JWST) Optical Telescope Element + Integrated Science Instrument Module (OTIS) went through environmental testing at the Goddard Space Flight Center. The vibration and acoustic tests occurred outside of cleanrooms. These areas are typically around ISO Class 8, far out of specifications for JWST. Through collaboration between the OTIS Mechanical and Contamination Engineering teams, a portable clean enclosure was designed, built, and verified to prevent contamination of the sensitive OTIS assembly. This manuscript describes the process of designing and building this enclosure, including materials selection and High Efficiency Particle Air (HEPA) filtration. This enclosure also provided environmental control with a portable air conditioning unit to control the relative humidity level enabling personnel to work within it. The manuscript emphasizes working in a systematic order to maintain cleanliness and integrity. “Clean as you go” during construction was a top priority to ensure that this critical Ground Support Equipment (GSE) was acceptable. The process of installing the walls and then sealing their internal and external surfaces with approved films was critical to maintaining OTIS integrity. This proved important because OTIS was in the enclosure for months longer than originally planned. This manuscript concludes by reviewing the process used to verify that the OTIS clean enclosure could achieve acceptable contamination level fallout accumulation for use during the vibration and acoustic testing and then verifying that the predicted performance was achieved after testing was complete.

Contamination Control, James Webb Space Telescope,↗