A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression
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59 BASIC BIOLOGICAL SCIENCES↗
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Results for “CRISPRi”
Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Auto-MuRCiS: a streamlined software package for analysis of multiplex, randomized CRISPR interference sequencing data
Multiplex, randomized CRISPR interference sequencing (MuRCiS) allows for the simultaneous identification of multiple gene knockouts that together influence microbial processes. Here, we report on an updated analysis tool called Auto-MuRCiS that utilizes Docker to make the analysis of these data rapid and more user-friendly.
59 BASIC BIOLOGICAL SCIENCES↗
Systems Biology to Enable Modular Metabolic Engineering of Fatty Acid Production in Cyanobacteria
This final technical report synthesizes major research results for DOE award DE-SC0022207. The project sought to identify metabolic control points and bottlenecks that regulate flux to free fatty acids (FFAs) in Picosynechococcus sp. PCC 7002 by treating lipid metabolism as modular pathways upstream and downstream of acetyl-CoA.
09 BIOMASS FUELS↗
Tn-seq chemical genomics in Zymomonas mobilis
Transposon sequencing chemical genomics to identify genes that impact the fitness of Zymomonas mobilis in a variety of chemicals
CRISPRi↗