Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Alternative Relativity”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 73 records · Page 4

Protein Motifs for Proton Transfers That Build the Transmembrane Proton Gradient

Biological membranes are barriers to polar molecules, so membrane embedded proteins control the transfers between cellular compartments. Protein controlled transport moves substrates and activates cellular signaling cascades. In addition, the electrochemical gradient across mitochondrial, bacterial and chloroplast membranes, is a key source of stored cellular energy. This is generated by electron, proton and ion transfers through proteins. The gradient is used to fuel ATP synthesis and to drive active transport. Here the mechanisms by which protons move into the buried active sites of Photosystem II (PSII), bacterial RCs (bRCs) and through the proton pumps, Bacteriorhodopsin (bR), Complex I and Cytochrome c oxidase (CcO), are reviewed. These proteins all use water filled proton transfer paths. The proton pumps, that move protons uphill from low to high concentration compartments, also utilize Proton Loading Sites (PLS), that transiently load and unload protons and gates, which block backflow of protons. PLS and gates should be synchronized so PLS proton affinity is high when the gate opens to the side with few protons and low when the path is open to the high concentration side. Proton transfer paths in the proteins we describe have different design features. Linear paths are seen with a unique entry and exit and a relatively straight path between them. Alternatively, paths can be complex with a tangle of possible routes. Likewise, PLS can be a single residue that changes protonation state or a cluster of residues with multiple charge and tautomer states.

cytochrome c oxidase↗

Transmission Alternatives for California North Coast Offshore Wind, Volume 4: Cost-Benefit Analysis Report

The purpose of this report is to compare the costs and benefits of installing offshore wind power plants with capacities up to 500 megawatts (MW) in the Humboldt Wind Energy Area offshore California, focusing on identifying the costs related to transmission upgrades and possible alternatives such as energy storage. We identify several options that result in lower costs than upgrading transmission to provide full deliverability. Our estimates of the levelized cost of energy (LCOE) assuming full deliverability range from $80/MWh for a 144-MW plant to $73/MWh for a 480-MW plant with a 2030 commercial operation date; however, the additional cost of transmission upgrades increases these values to between $86/MWh and $98/MWh. Based on curtailment and revenue estimates developed using a production cost model, the LCOEs for plant capacities of 144 MW and 168 MW with Energy Only deliverability are lower than the combined cost of energy and transmission upgrades for full deliverability. The addition of a 4-hour, 15-MW battery energy storage system increases revenue for both plant sizes, with the 144 MW + storage scenario producing the most revenue relative to LCOE.

17 WIND ENERGY↗

Natural Gas Vehicle Basics

Natural gas powers about 175,000 U.S. vehicles and more than 23 million vehicles worldwide.1 Natural gas vehicles (NGVs) are a good choice for high-mileage (high fuel-use) fleets - such as buses, medium- and heavy-duty trucks, and refuse vehicles - that are centrally fueled or operate within a limited area or along a route with natural gas fueling stations. The advantages of natural gas as an alternative fuel include its domestic production, established distribution network, relatively low cost, and emissions benefits.

alternative fuel↗

Spent Nuclear Fuel Exploratory Roadmaps - 20054

The U.S. Department of Energy manages nearly 2,500 metric tons of heavy metal (MTHM) of spent nuclear fuel (SNF) resulting from several decades of research, testing, and production reactors [DOE 1995]. This SNF is managed at the DOE Hanford Site in Washington State (Hanford), Idaho National Laboratory (INL), and Savannah River Site in South Carolina (SRS). From 1995 to 2004, the Department of Energy (DOE) made several key programmatic decisions, supported by appropriate documentation in accordance with the National Environmental Policy Act (NEPA) for the management of SNF [DOE 1995]. These decisions have provided an overarching framework for SNF management, as well as site-specific and SNF-related management decisions for the past two decades. In the years since these decisions were made, with the notable exception of the successful drying and packaging of the production reactor fuel at Hanford, a majority of the decisions have been largely unimplemented. Also, since these decisions were made, a number of changes that bear on considerations relative to the path forward have occurred. A SNF Exploratory Road map activity, identified reasonable alternate pathways for DoE's inventory of SNF. Three fundamental pathways were identified in this activity for the long-term management and disposition of DOE SNF: Direct Disposal Pathway, Existing Processing Pathway and Alternate Processing Pathway. Further actions would be required (e.g., evaluation of NEPA analysis, technology maturity evaluations, refinement of cost and risks, benefits and advantages relative to other alternate paths) to define and inform many key decisions that will result in the selection of the disposition pathways that are most beneficial to the US Government in dispositioning DoE's SNF inventory. DOE is currently managing all SNF safely; however, the age of DOE-owned SNF and facilities for storing and processing SNF, coupled with the uncertainty of the storage duration, necessitates decisions and actions to ensure that the infrastructure will be in place to ensure continued safe and effective long-term management and eventual disposal. The identification of reasonable alternate pathways took these considerations into account in an effort to proactively manage conditions that could challenge the safety of storing and managing DOE SNF over the time periods now contemplated and also to ensure that flexibility is preserved to ensure that the DOE SNF remains compatible with final disposition pathways when they become available. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗

Survey and Assessment of the Ocean Renewable Energy Resources in the US Gulf of Mexico

This study was conducted by the National Renewable Energy Laboratory (NREL) and funded by the Bureau of Ocean Energy Management (BOEM). It provides a comprehensive feasibility assessment of multiple offshore renewable energy technologies in the Gulf of Mexico (GoM) to inform BOEM's strategic plans related to possible Outer Continental Shelf alternative energy leasing activities in the GoM. In coordination with Gulf Coast states, the study includes some information on offshore renewable energy potential in state waters for future energy planning. The goal of the study is to survey potential offshore renewable energy sources in the GoM and quantify their feasibility relating to resource adequacy, technology maturity, and the potential for competitive cost. The study provides a review of available technologies and concepts for generating offshore renewable energy, including a high-level assessment of the current state of each technology and its potential for future advances. It provides a breakdown of resource capacity for each renewable energy technology and a recommendation that offshore wind be pursued for future study as the most promising technology. The renewable technologies that were considered include offshore wind, wave energy, tidal energy, ocean current energy, offshore solar energy, ocean thermal energy conversion (OTEC), cold water source cooling, and hydrogen (as a storage medium to utilize existing pipeline infrastructure). The resource capacity for each of these renewable energy sources was quantified for both the gross resource capacity potential (gross resource) and the technical resource capacity potential (technical resource) using the methodology described in an earlier NREL report. Many of these sources are very immature from a commercial perspective, which makes some of the comparisons difficult. In many cases, new methods were developed to estimate nominal power density for each technology type, which were necessary to convert the resource areas into deployable gross and technical resource capacity potentials.

17 WIND ENERGY↗

Study on Conversion from an Existing Alternating Current Medium-Voltage Line to a Direct Current Line: A Case of Load or Source Expansion

Simultaneous increase of power generation through renewable systems and load due owing to the rapid growth in electrified road transportation systems, leads to new challenges in the design, operation, and protection of distribution-level electrical power systems. This paper addresses challenges related to the integration of new sources or loads to a distribution grid through long alternating current lines. The International Council on Large Electric Systems medium-voltage benchmark model developed for North America was used for simulation studies. Use cases were developed to illuminate the issues related to long alternating current lines and the usefulness of medium-voltage direct current lines in a distribution network.

Jaldanki, Sreenivasa↗

In situ microbeam surface X-ray scattering reveals alternating step kinetics during crystal growth

The stacking sequence of hexagonal close-packed and related crystals typically results in steps on vicinal {0001} surfaces that have alternating A and B structures with different growth kinetics. However, because it is difficult to experimentally identify which step has the A or B structure, it has not been possible to determine which has faster adatom attachment kinetics. Here we show that in situ microbeam surface X-ray scattering can determine whether A or B steps have faster kinetics under specific growth conditions. We demonstrate this for organo-metallic vapor phase epitaxy of (0001) GaN. X-ray measurements performed during growth find that the average width of terraces above A steps increases with growth rate, indicating that attachment rate constants are higher for A steps, in contrast to most predictions. Overall, our results have direct implications for understanding the atomic-scale mechanisms of GaN growth and can be applied to a wide variety of related crystals.

36 MATERIALS SCIENCE↗

Safety-Related Instrumentation and Control Pilot Upgrade: Initial Scoping Phase Implementation and Lessons Learned

In May 2016, the U.S. Nuclear Regulatory Commission (NRC) staff provided a digital instrumentation and control (I&C) regulatory infrastructure integrated action plan to the NRC for approval. One of the objectives of that plan was to establish a clear regulatory structure with reduced regulatory uncertainty to enable the expanded safe use of digital I&C in commercial nuclear reactors while continuing to ensure safety and security. To achieve this end, the NRC, with collaboration from industry, developed a streamlined License Amendment Request Alternate Review (AR) process for safety-related (SR) digital I&C upgrades. In spite of this effort, the industry has remained reluctant to perform such I&C upgrades because of perceived regulatory and financial risks associated with being the first or an early adopter of the AR process for SR I&C upgrades. The U.S. Department of Energy Light Water Reactor Sustainability Program at the Idaho National Laboratory performed Initial Scoping Phase research to help break this impasse by supporting a SR I&C Pilot Upgrade, working with MPR Associates, ScottMadden Inc., and Exelon Generation. Exelon’s Limerick Generating Station (LGS) was selected as the target for this research. This paper summarizes the Initial Scoping Phase engineering and operations, licensing, and project management activities necessary to bound the scope, schedule, and estimated cost of the project sufficiently to enable utility management authorization of Conceptual Design Phase activities. These efforts and associated products are intended to provide a template to support larger industry efforts to perform similar upgrades as a foundation stone for a digital transformation that will improve plant safety, reliability, and operational performance while lowering plant total cost of ownership. As a result of the combined effort of Exelon Generation and research participants, Conceptual Design Phase activities for the subject upgrade at LGS were approved by Exelon. Further, the U.S. Department of Energy also awarded a $50 million cost share award to Exelon in order to pave the way for SR I&C modernization and associated control room upgrades across the U.S. nuclear fleet. Additional research reports are planned for the Conceptual Design Phase, Detailed Design Phase, and the Implementation Phase of the LGS project to document the process followed and promulgate lessons learned to industry.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Optimal Control and Architecture Design Optimization for Hydraulic Drive Train of a Compact Track Loader

Hydrostatic transmission (HST) is a common hydraulic drive train transmission used in track loaders and other high power off-road vehicles. While reliable, traditional HST with fixed-displacement motors has relatively poor efficiencies. In this paper, alternative HST and series hybrid drive train architectures are proposed to replace the traditional HST to allow the implementation of optimal control and optimization methods for better vehicle fuel efficiency. Besides the optimal control methods applied to minimize fuel consumption, the proposed architectures are also optimized for the best configuration in terms of component sizing. Backward-facing (acausal) simulation models were developed to estimate the fuel efficiency of each architecture. Finally, each architecture is compared for optimized performance and fuel saving relative to the traditional HST as baseline.

33 ADVANCED PROPULSION SYSTEMS↗

Perturbative quantum evolution of the gravitational state and dressing in general backgrounds

This paper sets up a perturbative treatment of the evolving quantum state of a gravitational system, in a Schrödinger-like picture, working about a general background. This connects gauge symmetry, the constraints, gravitational dressing, and evolution. Starting with a general time slicing, we give a simple derivation of the relation between the constraints, the Hamiltonian, and its well-known boundary term. Among different approaches to quantization with constraints, we focus on a “gauge-invariant canonical quantization,” which is developed perturbatively in the gravitational coupling. The leading-order solution of the constraints (including the Wheeler-DeWitt equation) for perturbations about the background is given in terms of an explicit construction of gravitational dressings built using certain generalized Green’s functions; different such dressings corresponding to adding propagating gravitational waves to a particular solution of the constraints. Dressed operators commute with the constraints, expressing their gauge invariance, and have an algebraic structure differing significantly from the undressed operators of the underlying field theory. These operators can act on the vacuum to create dressed states, and evolution of general such states is then generated by the boundary Hamiltonian, and alternately may be characterized using other relational observables. This provides a concrete approach to studying perturbative time evolution, including the leading gravitational backreaction, of quantum states of black holes with flat or anti–de-Sitter asymptotics, for example on horizon-crossing slices. This description of evolution in turn provides a starting point for investigating possibly important corrections to quantum evolution, that go beyond quantized general relativity. Published by the American Physical Society 2024

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Cryogenic Carbon Capture™ Technoeconomic Analysis

The Cryogenic Carbon Capture™ (CCC) process significantly decreases cost and energy demands for CO 2 separation and pressurization to 150 bar compared to alternatives. The process is a post-combustion technology that cools CO 2 -laden flue gas to desublimation temperatures (-100 to -135 °C), separates solid CO 2 —that forms from the flue gas—from the light gases, uses the cold products to cool incoming gases in a recuperative heat exchanger, compresses the solid/liquid CO 2 to final pressures (100–200 bar), and delivers a compressed CO 2 stream separated from an atmospheric pressure light-gas stream. The overall energy and economic costs are about 30-50% lower than most competing processes that involve air separation units (ASUs), solvents, or similar technologies. In addition, the CCC process enjoys several ancillary benefits, including (a) it is a minimally invasive Bolton technology, (b) it provides highly efficient removal of most pollutants (Hg, SOx, NO 2 , HCl, etc.), and (c) possible energy storage capacity. This report outlines the process details and economic and energy comparisons relative to other well-documented alternatives. This paper presents the results of a detailed techno-economic comparison of CCC with amine-based systems. The comparison uses identical financial and economic assumptions similar process assumptions as the detailed analyses published by US DOE in the greenfield analysis. Specifically, the comparison assumes power plants that produce the same net output, one equipped with and a second without carbon capture. Separately, the paper compares similar analyses for retrofitting existing systems using typical plant characteristics in the US (initial capital costs have been paid, high plant utilization), though there are no DOE estimates available for direct comparison. Financial and technical assumptions for all comparisons are maintained as close to the DOE reference studies as possible. The results demonstrate about 30-50% lower costs and energy demands for capture from greenfield coal plants. Natural gas plants produce substantially lower CO 2 concentrations which makes the cost of capturing a ton of CO 2 at the same capture rate as the coal plant higher for all processes while the cost of CO 2 capture per unit of power generation is lower. However, CCC maintains about the same absolute energy and cost advantages for NG as for coal compared to amine systems. Finally, the costs of retrofitting a station are compared to those of building a new station with and without capture. The retrofit costs are comparable to (slightly lower than) new plant costs without capture. In all cases operating and capital cost comparisons show that the CCC process can be retrofitted to a variety of plants to cost effectively reduce CO 2 emissions. Further process integration into the upstream processes and unique process features like water recovery, and integrated energy storage bring the effective cost of carbon capture using the CCC process down further and increase its advantages over alternatives. This technoeconomic analysis shows that the CCC process has the potential to the be lowest cost carbon capture technology under development today.

20 FOSSIL-FUELED POWER PLANTS↗

Anomalies in the topology of the temperature fluctuations in the cosmic microwave background: An analysis of the NPIPE and FFP10 data releases

We present a topological analysis of the temperature fluctuation maps from the Planck 2020 Data Release 4 NPIPE dataset and the Planck 2018 Data Release 3 FFP10 dataset. We performed a multiscale analysis in terms of the homology characteristics of the maps, invoking relative homology to account for the analysis in the presence of masks. We performed our analysis for a range of smoothing scales spanning sub- and super-horizon scales corresponding to a full width at half maximum (FWHM) of 5',10',20',40',80',160',320', and 640', and employed simulations based on the standard model for comparison, which assumes the initial fluctuation field to be an isotropic and homogeneous Gaussian random field. Examining the behavior of topological components, represented by the 0D homology group, we find the observations to be approximately 2σ or less deviant from the simulations for all resolutions and scales for the NPIPE dataset. For the FFP10 dataset, we detect a 2.96σ deviation between the observations and simulations at N = 128, FWHM = 80'. For the topological loops, represented by the first homology group, the simulations and observations are consistent within 2σ for most resolutions and scales for both the datasets. However, for the NPIPE dataset, we observe a high deviation between the observation and simulations in the number of loops at FWHM = 320', but at a low dimensionless threshold ν = –2.5. Under a Gaussian assumption, this would amount to a deviation of ~4σ. However, the distribution in this bin is manifestly non-Gaussian and does not obey Poisson statistics either. In the absence of a true theoretical understanding, we simply note that the significance is higher than what may be resolved by 600 simulations, yielding an empirical p-value of at most 0.0016. Specifically in this case, our tests indicate that the numbers arise from a statistically stable regime, despite being based on small numbers. For the FFP10 dataset, the differences are not as strong as for the NPIPE dataset, indicating a 2.77σ deviation at this resolution and threshold. The Euler characteristic, which is the alternating sum of the ranks of relative homology groups, reflects the deviations in the components and loops. To assess the significance of combined levels for a given scale, we employed the empirical and theoretical versions of the χ 2 test as well as the nonparametric Tukey depth test. Although all statistics exhibit a stable distribution, we favor the empirical version of the χ 2 test in the final interpretation, as it indicates the most conservative differences. For the NPIPE dataset, we find that the components and loops differ at more than 95%, but agree within the 99% confidence level with respect to the base model at N = 32, FWHM = 320'. The Euler characteristic at this resolution displays a per mil deviation. In contrast, the FFP10 dataset shows that the observations are consistent with the base model within the 95% confidence level, at this and smaller scales. This is consistent with the observations of the Planck analysis pipeline via Minkowski functionals. For the largest smoothing scale, N = 16, FWHM = 640', both datasets exhibit an anomalous behavior of the loops, where FFP10 data exhibit a deviation that is larger by an order of magnitude than that of the NPIPE dataset. In contrast, the values for the topological components and the Euler characteristic agree between observations and model to within a confidence level of 99%. However, for the largest scales, the statistics are based on low numbers and may have to be regarded with caution. Even though both datasets exhibit mild to significant discrepancies, they also exhibit contrasting behaviors at various instances. Therefore, we do not find it feasible to convincingly accept or reject the null hypothesis. Disregarding the large-scale anomalies that persist at similar scales in WMAP and Planck, observations of the cosmic microwave background are largely consistent with the standard cosmological model within 2σ.

79 ASTRONOMY AND ASTROPHYSICS↗

Pareto solutions in multicriteria optimization under uncertainty

We present and analyze several definitions of Pareto optimality for multicriteria optimization or decision problems with uncertainty primarily in their objective function values. In comparison to related notions of Pareto robustness, we first provide a full characterization of an alternative efficient set hierarchy that is based on six different ordering relations both with respect to the multiple objectives and a possibly finite, countably infinite or uncountable number of scenarios. We then establish several scalarization results for the generation of the corresponding efficient points using generalized weighted-sum and epsilon-constraint techniques. Finally, we leverage these scalarization results to also derive more general conditions for the existence of efficient points in each of the corresponding optimality classes, under suitable assumptions.

97 MATHEMATICS AND COMPUTING↗

Porous carbon from lignocellulosic biomass with emphasis on corn plant waste residue for energy storage

The rising global demand for sustainable energy storage materials has driven the search for environmentally friendly and cost-effective electrode options. Hydrothermal conversion of lignocellulosic biomass has gained attention due to its low energy requirements and operation at relatively low temperatures, presenting a green alternative to traditional thermochemical methods. The resulting solid product, hydrochar, has been used as an adsorbent and soil amendment; however, chemical/thermal treatment significantly enhances its physical properties. These structural modifications transform hydrochar into an effective porous carbon electrode, offering abundant sites for electrolyte ion transport, critical for high-performance devices like supercapacitors and batteries. This review first discusses various waste biomass and sustainable feedstocks available globally. It compares two primary thermochemical conversion techniques, pyrolysis and hydrothermal carbonization/liquefaction, and examines their respective solid products, biochar and hydrochar, analyzing differences in their physical and chemical characteristics. The focus is placed on hydrochar, summarizing activation methods to produce porous carbon suitable for energy storage applications. Additionally, this review will include a dedicated section on the application of porous carbon derived from corn plant waste residue, considering that corn is one of the most abundant crops grown worldwide, which makes it an important and promising source for sustainable porous carbon production. The role of machine learning models in optimizing hydrothermal processes to produce high-quality hydrochar is also discussed, emphasizing how data-driven approaches can streamline process development. Finally, the review identifies the current challenges and prospects for lignocellulosic biomass-derived porous carbon as a sustainable electrode material in next-generation energy storage technologies.

25 ENERGY STORAGE↗

Environmental life cycle assessment of treatment and management strategies for food waste and sewage sludge

A consequential life cycle assessment (LCA) was utilized to compare the environmental impacts of food waste and sewage sludge management strategies. The strategies included a novel two-phase anaerobic digestion (AD) system and alternatives including landfill, waste-to-energy, composting, anaerobic membrane bioreactor, and conventional AD (wet continuous stirred-tank reactor [CSTR]). The co-management of food waste with sewage sludge was also considered for the two-phase AD system and for a conventional AD reactor. Further, a multidimensional LCA approach was taken, considering the five-midpoint impact categories of global warming, smog, human health particulate, acidification, and eutrophication estimated using the U.S. EPA Tool for the Reduction and Assessment of Chemical and Other Environmental Impacts. Co-management of food waste and sewage sludge using the novel two-phase AD system was shown to maximize energy recovery and had a net global warming benefit while reducing other environmental impacts compared with the alternative management strategies. It had similar relative environmental advantages across all categories as conventional AD, with the advantage of a smaller physical footprint. However, both approaches featured net environmental burdens when the background electric grid intensity fell below 0.25 kg CO 2 -eq kWh -1 , as could be expected in a decarbonized electric future. Upgrading the biogas produced from AD to renewable natural gas can displace the use of fossil natural gas for other non-electricity energy requirements that are difficult to decarbonize and may extend the time period of significant environmental benefits of utilizing AD for organic waste management. Treatment of the nutrient-rich supernatant generated by the novel two-phase AD system could be an obstacle for utilities with stringent nutrient discharge limits. Future research and full-scale implementation are needed to demonstrate the benefits of the two-phase AD system predicted through this analysis.

54 ENVIRONMENTAL SCIENCES↗

Advanced electrode processing for lithium-ion battery manufacturing

Lithium-ion batteries (LIBs) need to be manufactured at speed and scale for their use in electric vehicles and devices. However, LIB electrode manufacturing via conventional wet slurry processing is energy-intensive and costly, challenging the goal to achieve sustainable, affordable and facile manufacturing of high-performance LIBs. Here, in this Review, we discuss advanced electrode processing routes (dry processing, radiation curing processing, advanced wet processing and 3D-printing processing) that could reduce energy usage and material waste. Maxwell-type dry processing is a scalable alternative to conventional processing and has relatively low manufacturing cost and energy consumption. Radiation curing processing could enable high-throughput manufacturing, but binder selection is limited to certain radiation curable chemistries. 3D-printing processing can produce electrodes with diverse architectures and improved rate performance, but scalability is yet to be demonstrated. 3D-printing processing is good for special applications where throughput and cost can be compromised for performance.

25 ENERGY STORAGE↗

Simplified Universal Equations for Ionic Conductivity and Transference Number

Nernst-Einstein equation can provide a reasonable estimate of the ionic conductivity of dilute solutions. For concentrated solutions, alternate methods such as Green–Kubo relations and Einstein relations are more suitable to account for ion-ion interactions. Such computations can be expensive for multicomponent systems. Simplified mathematical expressions like the Nernst-Einstein equation do not exist for concentrated multicomponent mixtures. Newman’s treatment of multicomponent concentrated solutions yields a conductivity relation in terms of species concentration and Onsager phenomenological coefficients. However, the estimation of these phenomenological coefficients is not straightforward. Here, mathematical formulations that relate the phenomenological coefficients with the friction coefficients are developed, leading to simplified, ready-to-use expressions of conductivity and transference numbers that can be used for a wide range of ionic mixtures. This approach involves spectral decomposition of the matrix of Onsager phenomenological coefficients. The general analytical expressions for conductivity and transference number are simplified for binary electrolytes, and numerical solutions are provided for ternary and quaternary mixtures with ion dissociation.

Electrochemistry↗

Metabolomic and transcriptomic remodeling of bone marrow myeloid cells in response to maternal obesity

Maternal obesity puts the offspring at high risk of developing obesity and cardiometabolic diseases in adulthood. Here, we utilized a mouse model of maternal high-fat diet (HFD)-induced obesity that recapitulates metabolic perturbations seen in humans. We show increased adiposity in the offspring of HFD-fed mothers (Off-HFD) when compared with the offspring of regular diet-fed mothers (Off-RD). We have previously reported significant immune perturbations in the bone marrow of newly weaned Off-HFD. Here, we hypothesized that lipid metabolism is altered in the bone marrow of Off-HFD versus Off-RD. To test this hypothesis, we investigated the lipidomic profile of bone marrow cells collected from 3-week-old Off-RD and Off-HFD. Diacylglycerols (DAGs), triacylglycerols (TAGs), sphingolipids, and phospholipids were remarkably different between the groups, independent of fetal sex. Levels of cholesteryl esters were significantly decreased in Off-HFD, suggesting reduced delivery of cholesterol. These were accompanied by age-dependent progression of mitochondrial dysfunction in bone marrow cells. We subsequently isolated CD11b+ myeloid cells from 3-wk-old mice and conducted metabolomic, lipidomic, and transcriptomic analyses. The lipidomic profiles of myeloid cells were similar to those of bone marrow cells and included increases in DAGs and decreased TAGs. Transcriptomics revealed altered expression of genes related to immune pathways, including macrophage alternative activation, B-cell receptors, and transforming growth factor-β signaling. All told, this study revealed lipidomic, metabolomic, and gene expression abnormalities in bone marrow cells broadly, and in bone marrow myeloid cells particularly, in the newly weaned offspring of mothers with obesity, which might at least partially explain the progression of metabolic and cardiovascular diseases in their adulthood.

RNA sequencing↗