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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 73 records · Page 4
All sourcesNASA NTRSDOE OSTI
DOE OSTI2020

Cas9 interrogates DNA in discrete steps modulated by mismatches and supercoiling

The CRISPR-Cas9 nuclease has been widely repurposed as a molecular and cell biology tool for its ability to programmably target and cleave DNA. Cas9 recognizes its target site by unwinding the DNA double helix and hybridizing a 20-nucleotide section of its associated guide RNA to one DNA strand, forming an R-loop structure. A dynamic and mechanical description of R-loop formation is needed to understand the biophysics of target searching and develop rational approaches for mitigating off-target activity while accounting for the influence of torsional strain in the genome. Here we investigate the dynamics of Cas9 R-loop formation and collapse using rotor bead tracking (RBT), a single-molecule technique that can simultaneously monitor DNA unwinding with base-pair resolution and binding of fluorescently labeled macromolecules in real time. By measuring changes in torque upon unwinding of the double helix, we find that R-loop formation and collapse proceed via a transient discrete intermediate, consistent with DNA:RNA hybridization within an initial seed region. Using systematic measurements of target and off-target sequences under controlled mechanical perturbations, we characterize position-dependent effects of sequence mismatches and show how DNA supercoiling modulates the energy landscape of R-loop formation and dictates access to states competent for stable binding and cleavage. Consistent with this energy landscape model, in bulk experiments we observe promiscuous cleavage under physiological negative supercoiling. The detailed description of DNA interrogation presented here suggests strategies for improving the specificity and kinetics of Cas9 as a genome engineering tool and may inspire expanded applications that exploit sensitivity to DNA supercoiling.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Time Projection Chamber (TPC) detectors for nuclear astrophysics studies with gamma beams

Gamma-Beams at the HIS facility in the USA and anticipated at the ELI-NP facility, now constructed in Romania, present unique new opportunities to advance research in nuclear astrophysics; not the least of which is resolving open questions in oxygen formation during stellar helium burning via a precise measurement of the 12C() reaction. Time projection chamber (TPC) detectors operating with low pressure gas (as an active target) are ideally suited for such studies. We look over the progress of the current research program and plans for the future at the HIS facility with the optical readout TPC (O-TPC) and the development of an electronic readout TPC for the ELI-NP facility (ELITPC).

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2023

Simulations and analysis tools for charge-exchange (d, 2 He) reactions in inverse kinematics with the AT-TPC

Charge-exchange (d, 2 He) reactions in inverse kinematics at intermediate energies are a very promising method to investigate the Gamow–Teller transition strength in unstable nuclei. A simulation and analysis software based on the attpcroot package was developed to study this type of reactions with the active-target time projection chamber (AT-TPC). The simulation routines provide a realistic detector response that can be used to understand and benchmark experimental data. Analysis tools and correction routines can be developed and tested from simulations in ATTPCROOT , because they are processed in the same way as the real data. In particular, we study the feasibility of using coincidences with beam-like particles to unambiguously identify the (d, 2 He) reaction channel, and to develop a kinematic fitting routine for future applications. More technically, the impact of space-charge effects in the track reconstruction, and a possible correction method are investigated in detail. Finally, this analysis and simulation package constitutes an essential part of the software development for the fast-beams program with the AT-TPC.

(d,2He)↗
DOE OSTI2025

Impact of pairing and neutron-excess on suppression of the above-barrier fusion cross-section in 19 O + 12 C

Fusion excitation functions for 19 O and 19 F + 12 C were simultaneously measured along with 20 O + 12 C using the active-target detector MuSIC@Indiana. Examination of the cross-section just above the barrier reveals a significant suppression of fusion for the 19 O + 12 C system starting at E 12 MeV. This suppression is not simply due to the reduced binding of the unpaired neutron in 19 O as 17 O + 12 C does not exhibit the same sharp suppression of the fusion cross-section. Multiple mean-field models are used as a reference to understand the measured fusion excitation functions.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗
DOE OSTI2025

Metal–Metal Bonding Influences Hydride Reactivity in [Sn–Rh] 3+ and [Sn–Ni] 2+ Bimetallics

Heavier group 14 metal hydrides serve as key intermediates in catalytic transformations, such as hydroboration. Regenerating such intermediates via a clean hydride source like dihydrogen could provide catalytic processes with a more economical alternative to, e.g., silanes and hydridoborane reagents. Herein, we report our efforts toward this goal using a [Sn–Rh] 3+ bimetallic system with the formal Rh I center acting as a potential dihydrogen activator. Targeting the introduction of a hydride ligand to the bimetallic core, our reactivity studies have revealed a preference for the hydride ligand to be bound to the Rh center, instead of the Sn center. Finally, examination of the electronic structures of these complexes via theoretical and experimental methods has revealed the electron acceptor nature of the Rh center within the bimetallic core and offers an explanation for the localization of the hydride between metal centers.

anions↗
DOE OSTI2022

Neutron-upscattering enhancement of the triple-alpha process

Abstract The neutron inelastic scattering of carbon-12, populating the Hoyle state, is a reaction of interest for the triple-alpha process. The inverse process (neutron upscattering) can enhance the Hoyle state’s decay rate to the bound states of 12 C, effectively increasing the overall triple-alpha reaction rate. The cross section of this reaction is impossible to measure experimentally but has been determined here at astrophysically-relevant energies using detailed balance. Using a highly-collimated monoenergetic beam, here we measure neutrons incident on the Texas Active Target Time Projection Chamber (TexAT TPC) filled with CO 2 gas, we measure the 3 α -particles (arising from the decay of the Hoyle state following inelastic scattering) and a cross section is extracted. Here we show the neutron-upscattering enhancement is observed to be much smaller than previously expected. The importance of the neutron-upscattering enhancement may therefore not be significant aside from in very particular astrophysical sites (e.g. neutron star mergers).

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗
DOE OSTI2024

Signatures of clustering accessible with a Time Projection Chamber: TexAT

Many experimental observables in clustering require high-sensitivity, almost background-free measurements. The use of Time Projection Chambers (TPCs) over the past 15 years have demonstrated their capability as a step change in the accessibility of many of these observables, as well as the possibility to study clustering via new techniques. A summary of the difficulties and pitfalls of determining the type of clustering from observables is briefly discussed, with a focus on α-condensation and the possibility of an additional (Efimov) 0 + state in 12 C, below the Hoyle state. The expansion of the technique used to tackle this challenge to study 3α + p clustering in 13 N is also discussed, highlighting the exciting opportunities TPCs provide to study clustering inaccessible by conventional means. Finally, an overview of the recent and upcoming advances in TPC technology is given as well as listing additional future challenges that are needed to be overcome, in the context of discussing the next-generation upgrade to the existing TexAT TPC, known as TeBAT (TExas Birmingham Active Target).

Bishop, Jack↗
DOE OSTI2025

Novel application of neutrinos to evaluate U.S. nuclear weapons performance

There is a growing realization that neutrinos can be used as a diagnostic tool to better understand the inner workings of a nuclear weapon. Robust estimates demonstrate that an Inverse Beta Decay (IBD) neutrino scintillation detector built at the Nevada Test Site with a 1000-ton active target mass at a standoff distance of 500 m would detect thousands of antineutrino events per nuclear test. This would provide less than 4% statistical error on the measured antineutrino rate and 5% error on antineutrino energy. Extrapolating this to an error on the test device explosive yield requires knowledge from evaluated nuclear databases, non-equilibrium fission rates, and assumptions on internal neutron fluxes. Initial calculations demonstrate that the total number of neutrinos emitted per fission in the first 10 3 s after a short pulse of 239 Pu fission is about a factor of two less than that from Pu fissioning under steady state conditions. Furthermore, there are significant energy spectral differences as a function of time after the pulse that must be considered. These and other model dependencies will be discussed in the paper. In the absence of nuclear weapons testing, many of the technical and theoretical challenges of a full nuclear test could be mitigated with a low cost smaller scale 20 ton fiducial mass IBD demonstration detector placed near a pulsed reactor. Potential reactors include the Texas A&M University TRIGA 1 GW–10 ms pulsed facility or the Sandia Annular Core Research Reactor. The short duty cycle and repeatability of pulses would provide critical real environment testing and measurements, which would be valuable for planning a possible real test shot in the future. Furthermore, the antineutrino rate as a function of time data would provide unique constraints on fission databases and model assumptions. Finally, there are impactful science drivers such as sensitive searches for ∼1 eV 2 sterile neutrinos and ∼MeV scale axions.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗
DOE OSTI2020

Design and performance of a 35-ton liquid argon time projection chamber as a prototype for future very large detectors

Liquid argon time projection chamber technology is an attractive choice for large neutrino detectors, as it provides a high-resolution active target and it is expected to be scalable to very large masses. Consequently, it has been chosen as the technology for the first module of the DUNE far detector. However, the fiducial mass required for “far detectors” of the next generation of neutrino oscillation experiments far exceeds what has been demonstrated so far. Scaling to this larger mass, as well as the requirement for underground construction places a number of additional constraints on the design. A prototype 35-ton cryostat was built at Fermi National Acccelerator Laboratory to test the functionality of the components foreseen to be used in a very large far detector. The Phase I run, completed in early 2014, demonstrated that liquid argon could be maintained at sufficient purity in a membrane cryostat. A time projection chamber was installed for the Phase II run, which collected data in February and March of 2016. The Phase II run was a test of the modular anode plane assemblies with wrapped wires, cold readout electronics, and integrated photon detection systems. While the details of the design do not match exactly those chosen for the DUNE far detector, the 35-ton TPC prototype is a demonstration of the functionality of the basic components. Measurements are performed using the Phase II data to extract signal and noise characteristics and to align the detector components. A measurement of the electron lifetime is presented, and a novel technique for measuring a track's position based on pulse properties is described.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗
DOE OSTI2024

A new hybrid gadolinium nanoparticles-loaded polymeric material for neutron detection in rare event searches

Experiments aimed at direct searches for WIMP dark matter require highly effective reduction of backgrounds and control of any residual radioactive contamination. In particular, neutrons interacting with atomic nuclei represent an important class of backgrounds due to the expected similarity of a WIMP-nucleon interaction, so that such experiments often feature a dedicated neutron detector surrounding the active target volume. In the context of the development of DarkSide-20k detector at INFN Gran Sasso National Laboratory (LNGS), several R&D projects were conceived and developed for the creation of a new hybrid material rich in both hydrogen and gadolinium nuclei to be employed as an essential element of the neutron detector. Thanks to its very high cross-section for neutron capture, gadolinium is one of the most widely used elements in neutron detectors, while the hydrogen-rich material is instrumental in efficiently moderating the neutrons. In this paper results from one of the R&Ds are presented. In this effort the new hybrid material was obtained as a poly(methyl methacrylate) (PMMA) matrix, loaded with gadolinium oxide in the form of nanoparticles. We describe its realization, including all phases of design, purification, construction, characterization, and determination of mechanical properties of the new material.

Dark Matter detectors (WIMPs, axions, etc.)↗
DOE OSTI2025

Measured gain suppression in FBK LGADs with different active thicknesses

In recent years, the gain suppression mechanism has been studied for large localized charge deposits in Low-Gain Avalanche Detectors (LGADs). LGADs are a thin silicon detector with a highly doped gain layer that provides moderate internal signal amplification. Using the CENPA Tandem accelerator at the University of Washington, the response of LGADs with different thicknesses to MeV-range energy deposits from a proton beam were studied. Three LGAD prototypes of 50 μm, 100 μm, and 150 μm were characterized. The devices' gain was determined as a function of bias voltage, incidence beam angle, and proton energy. This study was conducted in the scope of the PIONEER experiment, an experiment proposed at the Paul Scherrer Institute to perform high-precision measurements of rare pion decays. LGADs are considered for the active target (ATAR), and energy linearity is an important property for particle ID capabilities.

Particle tracking detectors↗
DOE OSTI2023

CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes

Abstract CRISPR-Cas enzymes enable RNA-guided bacterial immunity and are widely used for biotechnological applications including genome editing. In particular, the Class 2 CRISPR-associated enzymes (Cas9, Cas12 and Cas13 families), have been deployed for numerous research, clinical and agricultural applications. However, the immense genetic and biochemical diversity of these proteins in the public domain poses a barrier for researchers seeking to leverage their activities. We present CasPEDIA (http://caspedia.org), the Cas Protein Effector Database of Information and Assessment, a curated encyclopedia that integrates enzymatic classification for hundreds of different Cas enzymes across 27 phylogenetic groups spanning the Cas9, Cas12 and Cas13 families, as well as evolutionarily related IscB and TnpB proteins. All enzymes in CasPEDIA were annotated with a standard workflow based on their primary nuclease activity, target requirements and guide-RNA design constraints. Our functional classification scheme, CasID, is described alongside current phylogenetic classification, allowing users to search related orthologs by enzymatic function and sequence similarity. CasPEDIA is a comprehensive data portal that summarizes and contextualizes enzymatic properties of widely used Cas enzymes, equipping users with valuable resources to foster biotechnological development. CasPEDIA complements phylogenetic Cas nomenclature and enables researchers to leverage the multi-faceted nucleic-acid targeting rules of diverse Class 2 Cas enzymes.

59 BASIC BIOLOGICAL SCIENCES↗
DOE OSTI2020

Almost medium-free measurement of the Hoyle state direct-decay component with a TPC

The structure of the Hoyle state, a highly α -clustered state at 7.65 MeV in 12 C, has long been the subject of debate. Understanding if the system comprises of three weakly interacting α particles in the 0s orbital, known as an α-condensate state, is possible by studying the decay branches of the Hoyle state. The direct decay of the Hoyle state into three α particles, rather than through the 8 Be ground state, can be identified by studying the energy partition of the three α particles arising from the decay. This paper provides details on the breakup mechanism of the Hoyle stating using a new experimental technique. Method: By using β-delayed charged-particle spectroscopy of 12 N using the Texas active target time-projection chamber, a high-sensitivity measurement of the direct 3α decay ratio can be performed without contributions from pileup events. A Bayesian approach to understanding the contribution of the direct components via a likelihood function shows that the direct component is <0.043% at the 95% confidence level. This value is in agreement with several other studies, and, here, we can demonstrate that a small nonsequential component with a decay fraction of about 10 –4 is most likely. Here, the measurement of the non-sequential component of the Hoyle state decay is performed in an almost medium-free reaction for the first time. The derived upper limit is in agreement with previous studies and demonstrates sensitivity to the absolute branching ratio. Further experimental studies would need to be combined with robust microscopic theoretical understanding of the decay dynamics to provide additional insight into the idea of the Hoyle state as an α condensate.

6 ≤ A ≤ 19↗
DOE OSTI2021

Evidence against the Efimov effect in 12 C from spectroscopy and astrophysics

Background: The Efimov effect is a universal phenomenon in physics whereby three-body systems are stabilized via the interaction of an unbound two-body subsystems. A hypothetical state in 12 C at 7.458-MeV excitation energy, comprising a loose structure of three α particles in mutual two-body resonance, has been suggested in the literature to correspond to an Efimov state in nuclear physics. The existence of such a state has not been demonstrated experimentally. Purpose: Using a combination of γ spectroscopy, charged-particle spectroscopy, and astrophysical rate calculations allowing for strict limits on the existence of such a state to been established here. Method: Using the combined data sets from two recent experiments, one with the TexAT (Texas Active Target) TPC (Time Projection Chamber) to measure α decay and the other with Gammasphere to measure γ decay of states in 12 C populated by 12 N and 12 B β decay, respectively, we achieve high sensitivity to states in close proximity to the α threshold in 12 C. Results: No evidence of a state at 7.458 MeV is seen in either data set. Using a likelihood method, the 95% confidence limit γ -decay branching ratio is determined as a function of the β-decay feeding strength relative to the Hoyle state. In parallel, calculations of the 3α reaction rate show the inclusion of the Efimov corresponds to a large increase in the reaction rate around 5 × 10 7 K. Conclusion: From decay spectroscopy—at the 95% confidence limit, the Efimov state cannot exist at 7.458 MeV with any γ-decay branching ratio unless the β strength is less than 0.7% of the Hoyle state. This limit is evaluated for a range of different excitation energies and the results are not favorable for existence of the hypothetical Efimov state in 12 C . Furthermore, the 3α reaction rate with the inclusion of a state between 7.43 and 7.53 MeV exceeds the rate required for stars to undergo the red giant phase.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2022

First direct measurement of the 13 $\mathrm{N(α, ρ)}$ 16 $\mathrm{O}$ reaction relevant for core-collapse supernovae nucleosynthesis

We report that understanding the explosion mechanism of a core-collapse supernova (CCSN) is important to accurately model CCSN scenarios for different progenitor stars using model-observation comparisons. The uncertainties of various nuclear reaction rates relevant for CCSN scenarios strongly affect the accuracy of these stellar models. Out of these reactions, the 13 N(α, p) 16 O reaction has been found to affect various stages of a CCSN at varying temperatures. This work presents the first direct measurement of the 13 N(α, p) 16 O reaction performed using a 34.6 MeV beam of radioactive 13 N ions and the active-target detector MUSIC (MUlti-Sampling Ionization Chamber) at Argonne National Laboratory. The resulting total 13 N(α, p) 16 O reaction cross sections from this measurement in the center-of-mass energy range of 3.26 - 6.02 MeV are presented and compared with calculations using the Hauser-Feshbach formalism. Uncertainties in the reaction rate have been dramatically reduced at CCSN temperatures.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2022

α-cluster structure of 18 Ne

In this work, we investigate α-clustering in 18 Ne and compare it with what is known about clustering in the mirror nucleus 18 O. The excitation function for resonant elastic scattering of 14 O + α was measured in inverse kinematics using the TexAT active-target detector system, covering excitation energies from 8 to 17 MeV. The data were analyzed using a multi-channel R-matrix approach. Detailed spectroscopic information—including excitation energies, spin and parity assignments, and partial α and total widths—was extracted from the R-matrix analysis and compared with theoretical models and previously available data. Correspondence between levels in 18 O and 18 Ne is established. An extensive shell-model analysis of the 18 O– 18 Ne mirror pair shows very good agreement between theory and experiment, especially in the distribution of clustering strength. The comparison reveals that certain states, particularly at higher excitation energies, exhibit significantly stronger α-cluster character than predicted. This suggests that these states have a collective structure aligned with the corresponding α-reaction channel.

6 ≤ A ≤ 19↗
DOE OSTI2023

Quantifying resonance behavior in the fusion of O 17 with C 12 at above-barrier energies

Recent measurement of the fusion excitation function for 17 O + 12 C reported the significant suppression of the fusion cross section at E c.m. ≈ 14 MeV. This suppression was hypothesized to signal the existence of a 16 O + n + 12 C molecular configuration. Using the active-target detector MuSIC@Indiana provided an effective means of reexamining the fusion excitation function for 17 O + 12 C.The accuracy of this thick-target measurement is strengthened through comparison with the thin-target measurement of the excitation function for 17 F + 12 C. Furthermore, the result provides important information about the dependence of the average fusion cross section for the oxygen isotopic chain on neutron excess.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗
DOE OSTI2024

Cluster structure of 3⁢𝛼+𝑝 states in 13 N

Cluster states in 13 N are extremely difficult to measure due to the unavailability of 9 B +𝛼 elastic-scattering data. Using 𝛽-delayed charged-particle spectroscopy of 13 O, clustered states in 13 N can be populated and measured in the 3⁢𝛼+𝑝 decay channel. One-at-a-time implantation and decay of 13 O was performed with the Texas Active Target Time Projection Chamber. 149⁢𝛽⁢3⁢𝛼⁢𝑝 decay events were observed and the excitation function in 13 N reconstructed. Four previously unknown 𝛼-decaying excited states were observed in 13 N at an excitation energy of 11.3, 12.4, 13.1, and 13.7 MeV decaying via the 3⁢𝛼+𝑝 channel. These states are seen to have a [ 9 B ⁡(g.s) ⁢⨂𝛼/𝑝 + 12 C ⁡(0$^+_2$)], [ 9 B ⁡($\frac{1}{2}$ + )⁢ ⨂𝛼], [ 9 B ⁡($\frac{5}{2}$ + )⁢ ⨂𝛼], and [ 9 B⁡ ($\frac{5}{2}$) ⁢⨂𝛼] structure, respectively. A previously seen state at 11.8 MeV was also determined to have a [𝑝+ 12 C ⁡(g.s.)/𝑝+ 12 C ⁡(0$^+_2$)] structure. The overall magnitude of the clustering is not able to be extracted, however, due to the lack of a total width measurement. Clustered states in 13 N (with unknown magnitude) seem to persist from the addition of a proton to the highly 𝛼-clustered 12 C . Evidence of the $\frac{1}{2}$ + state in 9 B was also seen to be populated by decays from 13 N ★ .

Physics↗