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At least 703 records · Page 39

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NASA NTRS1989

Investigations on gel forming media use in low gravity bioseparations research

Research on gelling media and conditions suitable for the preservation of the spatial configuration of cell suspensions and macromolecular solutions after separation in free fluid during low gravity experiments is presented. The examples studied included free electrophoresis of cells in a cylindrical column and two-phase aqueous polymer separation. Microgravity electrophoresis experiments were simulated by separating model cell types (animal or human) in a vertical density gradient containing low-conductivity buffer, 1.7-6.5 percent Ficoll, 6.8-5.0 percent sucrose, and 1 percent SeaPrep low-melting temperature agarose. Upon cooling, a gel formed in the column and cells could be captured at the forming locations. Two-phase extraction experiments were simulated using two-polymer solutions in which phase separation occurs in normal saline at temperatures compatible with cell viability and in which one or both phases form a gel upon cooling. Suitable polymers included commercial agaroses (1-2 percent), maltodextrin (5-7 percent), and gelatin (5-20 percent).

Todd, Paul↗

NASA NTRS1989

Remote sensing and numerical modeling of suspended sediment in Laguna de Terminos, Campeche, Mexico

It is necessary to understand the complex physical processes at work in coastal lagoons in order to manage them effectively. Improved methods of data collection and analysis must be found to provide synoptic, timely hydrodynamic information because of the sheer size of some lagoons and the difficulty of acquiring in situ data (particularly in the tropics). This paper summarizes research to model salinity and suspended sediment distributions in Laguna de Terminos, Mexico, using (1) a coupled hydrodynamic and dispersion model and (2) analysis of two Landsat Thematic Mapper images collected on November 25, 1984 and April 24, 1987. Atmospherically corrected chromaticity data derived from Thermatic Mapper data were significantly correlated with modeled total suspended sediment concentrations for the two dates. Comparison between numerically modeled and remotely sensed suspended sediment maps at 1.5 x 1.5 km resolution yielded a covariation map useful for identifying areas of discrepancy between the remotely sensed data and model output.

Jensen, John R.↗

NASA NTRS1989

The plume of the Yukon River in relation to the oceanography of the Bering Sea

The ecosystem of the northern Bering-Sea shelf was studied using data from the NOAA Very High Resolution Radiometer and AVHRR and the Landsat MSS and Thematic Mapper (TM) in conjunction with shipboard measurements. Emphasis was placed on the influence of the Yukon River on this inner shelf environment and on the evaluation of the utility of the new Landsat TM data for oceanography. It was found that the patterns of water mass distribution obtained from satellite images agreed reasonably well with the areal patterns of temperature, salinity, and phytoplankton distributions. The AVHRR, MSS, and TM data show that the Yukon-River discharge is warmer and more turbid than the surrounding coastal water that originates to the south; thus, the Yukon water contributes to the higher temperatures and lower transmissivity of the coastal water. The high resolution of the TM thermal IR band made it possible to observe complex patterns and structures in the surface water that could not be resolved on previous data sets.

Dean, Kenneson G.↗

NASA NTRS1990

Therapeutic effects of antimotion sickness medications on the secondary symptoms of motion sickness

In addition to nausea and vomiting, motion sickness involves slowing of brain waves, loss of performance, inhibition of gastric motility and the Sopite Syndrome. The therapeutic effects of antimotion sickness drugs on these reactions were evaluated. The subjects were rotated to the M-III end-point of motion sickness. Intramuscular (IM) medications were then administered. Side effects before and after rotation were reported on the Cornell Medical Index. Brain waves were recorded on a Grass Model 6 Electroencephalograph (EEG), and gastric emptying was studied after an oral dose of 1 mCi Technetium 99m DTPA in 10 oz. isotonic saline. An increase in dizziness and drowsiness was reported with placebo after rotation. This was not prevented by IM scopolamine 0.1 mg or ephedrine 25 mg. EEG recordings indicated a slowing of alpha waves with some thea and delta waves from the frontal areas after rotation. IM ephedine and dimenhydrinate counteracted the slowing while 0.3 mg scopolamine had an additive effect. Alterations of performance on the pursuit meter correlated with the brain wave changes. Gastric emptying was restored by IM metoclopramide. Ephedrine IM but not scopolamine is effective for some of the secondary effects of motion sickness after it is established.

Wood, C. D.↗

NASA NTRS1990

Aerospace video imaging systems for rangeland management

This paper presents an overview on the application of airborne video imagery (VI) for assessment of rangeland resources. Multispectral black-and-white video with visible/NIR sensitivity; color-IR, normal color, and black-and-white MIR; and thermal IR video have been used to detect or distinguish among many rangeland and other natural resource variables such as heavy grazing, drought-stressed grass, phytomass levels, burned areas, soil salinity, plant communities and species, and gopher and ant mounds. The digitization and computer processing of VI have also been demonstrated. VI does not have the detailed resolution of film, but these results have shown that it has considerable potential as an applied remote sensing tool for rangeland management. In the future, spaceborne VI may provide additional data for monitoring and management of rangelands.

Everitt, J. H.↗

NASA NTRS1990

Satellite observations of the ice cover of the Kuril Basin region of the Okhotsk Sea and its relation to the regional oceanography

For the period 1978-1982, this paper examines the nature of the sea ice which forms over the Kuril Basin of the Okhotsk Sea and describes the impact of this ice on the regional oceanography. The oceanographic behavior during the heavy ice season associated with the cold 1979 winter is compared with the behavior during the lighter ice years of 1980 and 1982. Examination of the oceanography in the Okhotsk and the adjacent Pacific shows that the early summer water column structure depends on the heat loss from the Okhotsk during the preceding ice season, the total amount of Okhotsk ice formation, and, specifically, the amount of the ice formation in the Kuril Basin. Following the 1979 ice season, the upper 200-300 m of the Kuril Basin waters were cooler, less saline, and richer in oxygen than for the other years. This modification appears to be a process local to the Kuril Basin, driven by eddy-induced mixing, local cooling, and ice melting.

Wakatsuchi, Masaaki↗

NASA NTRS1990

Characterization of blood drawn rapidly for use in blood volume expansion studies: An animal model for simulated weightlessness

It was demonstrated that up to 8ml of blood can be drawn from donar rats without significantly increasing volume and stress sensitive hormones, and thus can be used for volume expansion studies. Infusion of whole blood allows more physiological changes that can be seen with volume expansion by saline or other ionic solutions. The infusion of whole blood to induce hypervolemia may provide an improved model to study the fluid balance and control mechanisms operative in weightlessness. Blood samples were drawn as quickly as possible from femoral artery catheters chronically implanted in Sprague Dawley rats and analyzed for hematocrit, plasma sodium, potassium, osmolality, corticosterone, epinepherine, norepinephrine, and vasopressin. The levels were found to be comparable to those of normal rats.

Chenault, V. Michelle↗

NASA NTRS1991

Survival of epiphytic bacteria from seed stored on the Long Duration Exposure Facility (LDEF)

This study was designed to determine the survival of microorganisms exposed to the relatively harsh conditions found in low Earth orbit (LEO). Seed of corn, sunflower, canteloupe, zucchini, bean, pea, and pumpkin cultivars were packaged in two 18 x 2.5 cm aluminum tubes; wall thickness for each tube was 1.33 mm. One seed tube was attacked to payload M0006, tray C-2; a second tube was stored at room temperature in a lab on Earth. Five lithium fluoride thermoluminescent dosimetry wafers (TLD-100 wafers) were placed in each aluminum tube. The total mean dosages for flight and ground-control TLD wafers were 210.0 and 0.9 rads, respectively. Seeds were washed for 2 hrs in a phosphate buffered saline solution. Bacteria were isolated by plating samples of the seed-washings onto dilute tryptic soy agar. Pure isolates of morphologically distinct bacteria were obtained by standard microbiological procedures. Bacteria were grouped according to colony-type and preliminary identification was completed using a fatty-acid analysis system. Bacillus spp. were the primary microoganisms that survived on seed during the experiment. Bacterial diversity and relative abundance were similar for the ground flight seed. Bacillus subtilus, B. pumilus, B. licheniformis, B. polymyxa, B. megaterium, and B. pabuli were isolated most frequently. Members of the genera Kurthia, Listeria, Micrococcus, and Arthrobacter were also isolated from flight and ground control seed. Results support the hypothesis that terrestrial microorganisms can survive long periods of time in the relatively harsh LEO environment.

Schuerger, Andrew C.↗

NASA NTRS1989

Rivers in the sea - Can we quantify pigments in the Amazon and the Orinoco River plumes from space?

Coastal Zone Color Scanner (CZCS) images of the western tropical Atlantic (1979-1982) were combined into monthly mean surface pigment fields. These suggest that Amazon River water flows along northeastern South America directly toward the Caribbean sea early in the year. After June, however, the North Brazil Current is shunted eastward, carrying a large fraction of Amazon water into the North Equatorial Countercurrent (NECC). This eastward flow causes diminished flow through the Caribbean, which permits northwestward dispersal of Orinoco River water due to local Ekman forcing. The Orinoco plume crosses the Caribbean, leading to seasonal variation in surface salinity near Puerto Rico. At least 50 percent of the pigment concentration estimated in these plumes seems due to viable phytoplankton.

Muller-Karger, Frank E.↗

NASA NTRS1991

Intranasal scopolamine preparation and method

A new method and preparation for intranasal delivery of scopolamine provides a safe and effective treatment for motion sickness and other conditions requiring anticholinergic therapy. The preparation can be in the form of aqueous nasal drops, mist spray, gel or oinment. Intranasal delivery of scopolamine has similar bioavailability and effect of intravenous delivery and is far superior to oral dosage. Scopolamine is prepared in a buffered saline solution at the desired dosage rate for effective anticholinergic response.

Putcha, Lakshmi↗

NASA NTRS1991

Hoop column soil moisture spacecraft in low Earth orbit for global change monitoring

A subset of the total Global Change Technology Initiative instruments are required to be in low Earth, sunsynchronous orbits. There is one instrument, however, that requires its own specialized spacecraft; the Soil Moisture Microwave Radiometer (SMMR). The characteristic structure of the instrument is the 118 m hoop column support structure. The hoop is supported by an axially placed column. Tension cables support and shape an electromagnetically reflective mesh surface. The instrument is capable of detecting frequencies in the 1.4 GHz range (Soil Moisture and Sea Salinity). Three apertures are used to reduce the degree of paraboloid offset and improve the beam quality. The spacecraft configuration is determined by the instrument support requirements and the requirement that it can fit into the Titan IV cargo bay. The configuration is derived by cross referencing the instrument performance requirements with the performance of the spacecraft. The spacecraft design is similar with the Multi-mission Modular Spacecraft in terms of size and packaging. A description of the spacecraft's features will yield a summary of the technologies needed for the SMMR spacecraft.

Ferebee, Melvin J., Jr.↗

NASA NTRS1991

Corrosion-protective coatings from electrically conducting polymers

In a joint effort between NASA Kennedy and LANL, electrically conductive polymer coatings were developed as corrosion protective coatings for metal surfaces. At NASA Kennedy, the launch environment consist of marine, severe solar, and intermittent high acid and/or elevated temperature conditions. Electrically conductive polymer coatings were developed which impart corrosion resistance to mild steel when exposed to saline and acidic environments. Such coatings also seem to promote corrosion resistance in areas of mild steel where scratches exist in the protective coating. Such coatings appear promising for many commercial applications.

Thompson, Karen Gebert↗

NASA NTRS1992

In vitro measurement of nucleus pulposus swelling pressure: A new technique for studies of spinal adaptation to gravity

Swelling of the intervertebral disc nucleus pulposus is altered by posture and gravity. We have designed and tested a new osmometer for in vitro determination of nucleus pulposus swelling pressure. The functional principle of the osmometer involves compressing a sample of nucleus pulposus with nitrogen gas until saline pressure gradients across a 0.45 microns Millipore filter are eliminated. Swelling pressure of both pooled dog and pooled pig lumbar disc nucleus pulposus were measured on the new osmometer and compared to swelling pressures determined using the equilibrium dialysis technique. The osmometer measured swelling pressures comparable to those obtained by the dialysis technique. This osmometer provides a rapid, direct, and accurate measurement of swelling pressure of the nucleus pulposus.

Hargens, A. R.↗

NASA NTRS1991

Cardiopulmonary responses to acute hypoxia, head-down tilt and fluid loading in anesthetized dogs

Cardiopulmonary responses to acute hypoxia (HY), fluid loading by saline infusion (FL), and head-down tilt (HD) of mechanically ventilated anesthetized dogs were investigated by measuring thermodynamics and pulmonary gas exchange. It was found that HD decreased the total respiratory compliance both during HY and normoxia (NO) and that the reduction in compliance by FL was twice as large as by HD. Superimposing HD on HY doubled the increase in vascular resistance due to HY alone. In the systemic circulation, HD lowered the resistance to below NO levels. There was a significant positive correlation between the changes in blood volume and in pulmonary artery pressure for experimental transitions, suggesting that a shift in blood volume from systemic to pulmonary circulations and changes in the total blood volume may contribute substantially to these apparent changes in resistance.

Loeppky, J. A.↗

NASA NTRS1991

Diurnal changes in the dielectric properties and water status of eastern hemlock and red spruce from Howland, ME

The diurnal characteristics of microwave dielectric properties and water potential of two conifer species were investigated in July and September, 1990. P-band and C-band radial dielectric profiles of hemlock and red spruce, as well as hemlock diurnal water potential and dielectric profiles, are presented. The resulting radial dielectric profiles matched the regions of the functional sapwood (water transport component of the active xylem) in both species such that the sapwood was characterized by a higher dielectric than the bark and heartwood tissues. This is probably due to characteristic differences in the water content of each tissue. As the hemlocks progressed through their diurnal water potential pattern, the dielectric profile remained static until mid-afternoon. As the tension in the water column relaxed (2 to 3 bars) the dielectric constant decreased by 30 to 40 percent. There are several possible explanations for this phenomenon, and these may relate to the dependency of the dielectric measurements on temperature, salinity, and volumetric water content.

Salas, W. A.↗

NASA NTRS1992

A southwest monsoon hydrographic climatology for the Northwestern Arabian Sea

This paper provides a detailed hydrographic climatology for the shallow northwestern Arabian Sea prior to and during the southwest monsoon, presented as multiple-year composite vertical hydrographic sections based on National Oceanographic Data Center historical ocean station data. Temperature and salinity measurements are used to infer the water masses present in the upper 500 m. The hydrographic evolution depicted on bimonthly sections is inferred to result from wind-driven physical processes. In the northwestern Arabian Sea the water mass in the upper 50 m is the Arabian Sea Surface Water. Waters from 50 to 500 m are formed by mixing of Arabian Sea Surface Water with Antarctic and Indonesian intermediate waters. The inflow of Persian Gulf Water does not significantly influence the hydrography of the northwestern Arabian Sea along the Omani coast. Nitrate has a high inverse correlation with temperature and oxygen in the premonsoon thermocline in the depth interval 50-150 m. During the southwest monsoon, coastal upwelling off Oman and adjacent offshore upward Ekman pumping alter the shallow hydrography.

Brock, John C.↗

NASA NTRS1992

Transport of the Norwegian Atlantic current as determined from satellite altimetry

Relatively warm and salty North Atlantic surface waters flow through the Faeroe-Shetland Channel into the higher latitudes of the Nordic Seas, preserving an ice-free winter environment for much of the exterior coast of northern Europe. This flow was monitored along the Norwegian coast using Geosat altimetry on two ascending arcs during the Exact Repeat Mission in 1987-1989. Concurrent undertrack CTD surveys were used to fix a reference surface for the altimeter-derived SSH anomalies, in effect creating time series of alongtrack surface dynamic height topographies. Climatologic CTD casts were then used, with empirical orthogonal function (EOF) analysis, to derive relationships between historical surface dynamic heights and vertical temperature and salinity profiles. Applying these EOF relationships to the altimeter signals, mean transports of volume, heat, and salt were calculated at approximately 2.9 Sverdrups, 8.1 x 10 exp 11 KCal/s and 1.0 x 10 exp 8 Kg/s, respectively. Maximum transports occurred in February/March and minimum in July/August.

Pistek, Pavel↗

NASA NTRS1992

A history of the Lonar crater, India: An overview

The origin of the circular structure at Lonar, India, described variously as cauldron, pit, hollow, depression, and crater, has been a controversial subject since the early nineteenth century. A history of its origin and other aspects from 1823 to 1990 are overviewed. The structure in the Deccan Trap Basalt is nearly circular with a breach in the northeast, 1830 m in diameter, 150 m deep, with a saline lake in the crater floor. Over the years, the origin of the Lonar structure has risen from volcanism, subsidence, and cryptovolcanism to an authentic meteorite impact crater. Lonar is unique because it is probably the only terrestrial crater in basalt and is the closest analog with the Moon's craters. Some unresolved questions are suggested. The proposal is made that the young Lonar impact crater, which is less than 50,000 years old, should be considered as the best crater laboratory analogous to those of the Moon, be treated as a global monument, and preserved for scientists to comprehend more about the mysteries of nature and impact cratering, which is now emerging as a fundamental ubiquitous geological process in the evolution of the planets.

Nayak, V. K.↗