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At least 703 records · Page 39
All sourcesNASA NTRSDOE OSTI
NASA NTRS2026

Does the International Space Station Leak DNA? Preliminary Results from the ISS External Microorganisms Payload

Existing crewed spacecraft like the ISS (International Space Station) leak by design. The ISS routinely releases gas to maintain life support systems and when astronauts exit the station to perform space walks. The chemical component of this leakage is well characterized, but the biological components are not. The ISS is not subject to planetary protection requirements, but planned missions to Mars will use similar systems and will be subject to planetary protection requirements. If detectable microorganisms are escaping through vents and or airlocks we may need to redesign our crewed habitats to minimize this type of contamination. To test the hypothesis that microorganisms from inside ISS are detectable on exterior surfaces an astronaut used the ISS External Microorganisms sampling kit (Rucker et al. 2018) to sample exterior surfaces of the ISS during an EVA (Extra Vehicular Activity) in January of 2025. These samples were returned to Earth for DNA extraction and sequencing. We successfully, extracted and sequenced bacterial, fungal and viral DNA from these samples that was not present in the negative controls. These results should help NASA refine the planetary protection requirements for crewed missions. Methods: The samples were collected using sterile, DNA free, buccal swabs (23 mm. diameter) housed in custom canisters. Each canister uses a 0.2 μm Teflon filter to maintain sterility as the caddy, holding 8 swabs moves in and out of vacuum. The astronaut sampled the: 1) airlock vestibule, 2) airlock thermal cover, 3) a gap in the micrometeorite shielding near the airlock, 4) a handrail near the airlock, 5) the Carbon Dioxide Removal Assembly vent, and 6) the Vacuum Exhaust System vent. The seventh swab was exposed to vacuum during the EVA without touching it to a surface. The eighth swab, a negative control, was not opened until the caddy returned to Earth. DNA was extracted from the swabs using a QIamp UCP Pathogen kit and prepared for sequencing on an Aviti (Element Biosciences) sequencer (Arslan et al. 2024). The resulting sequences were analyzed using the EDGE Bioinformatics platform (Li et al. 2017). The sequences were analyzed individually using tools like BLAST, GOTTCHA2, Kraken2, and PanGIA. The data were also assembled into metagenome assembled genomes) using tools like CONCOCT, MaxBin2 and MetaBAT2. Results: We successfully extracted and sequenced bacterial, archaeal, fungal and viral DNA from all seven samples. The handrail swab had the lowest number of reads (768,651) and the airlock thermal cover had the highest number of reads (8,819,230). These samples contain DNA from human associated bacteria (e.g. Crynebacterium riegelii ), fungi (.e.g. Penicillium rubens ), and viruses (e.g Alphapapillomavirus ). Conclusion: Preliminary interpretation suggest that the airlock and the space suits themselves are the largest sources of contaminant DNA. Most if not all of the DNA is from organisms known to be present inside the ISS. Vents attached to life support systems may be a lesser source of biological contamination. Further analysis should help NASA address planetary protection knowledge gaps for crewed missions.

Aaron B Regberg↗
NASA NTRS2018

Testing the Martian Methane from Cometary Debris Hypothesis: The Unusually Close 24 Jan 2018 Interaction Between Comet C/2007 H2 (Skiff) and Mars

In previous work we proposed a hypothesis wherein debris moving along cometary orbits interacting with Mars (e.g. meteor showers) may be responsible for transient local increases of methane observed in the martian atmosphere (henceforth 'the hypothesis' ). An examination of the literature of methane detections dating back to 1997 showed that each detection was made, at most, 16 days after an interaction between Mars and one of seven small bodies (six comets and the unusual object 5335 Damocles)[ibid]. Two observations of high-altitude, transient visible plumes on Mars also correlate with cometary interactions, one occurring on the same day as the plume observation and the second observation occurring three days afterwards, and with two of the same seven small bodies. The proposed mechanism for methane production is dissemination of carbon-rich cometary material on infall into Mars' atmosphere followed by methane production via UV photolysis, a process that has been observed in laboratory experiments. Given this set of observations it is necessary and indeed conducive to the scientific process to explore and robustly test the hypothesis.

Fries, M.↗
NASA NTRS1983

Paired quasars near NGC 2639 - Evidence for quasars in superclusters

Arp (1979, 1980) has found quasars with similar redshifts which appear to be paired across low-redshift galaxies. Arp concludes that the pairings provide evidence for an association between high-redshift quasars and a small, low-redshift galaxy. Oort et al. (1981) suggested an alternative hypothesis to explain the close redshift pairs. They proposed that the two closest pairs are in superclusters at the cosmological distances implied by the quasars' redshifts. The low-redshift quasar pair U7/U10 (0.303/0.305) is close enough to allow detection of any associated clusters of galaxies on deep red photographs. The present investigation had originally the objective to test the supercluster hypothesis by searching for faint clusters which might comprise a supercluster at z approximately 0.3. Unfortunately, the disappearance of the pairs makes it impossible to test the hypothesis in this field. The search for a supercluster at z = 0.30 revealed a faint rich cluster of galaxies near the quasar U10 (z = 0.305). It was found that U10 is not associated with the cluster.

Ford, H.↗
NASA NTRS1998

Final Report

The specific aims of the research were two fold: 1) to test the working hypothesis that space flight can alter normal development of vestibular sense organs and 2) to characterize adaptation of vestibular receptors to weightlessness and readaptation upon the return to earth. The proposed research has two scientific objectives: 1) In hatchlings (and perhaps embryos): to critically test the hypothesis that space flight can modify the ontogeny of vestibular gravity receptors, and 2) In adult birds: to characterize adaptation of vestibular gravity receptors to weightlessness and further to characterize re-adaptation of vestibular gravity receptors upon their return to earth.

Jones, Timothy A.↗
NASA NTRS2024

The Potassium Isotope Composition of Aggregate Material From Asteroid Bennu

NASA’s OSIRIS-REx mission re-turned material from asteroid Bennu on September 24, 2023, marking the first time a U.S. mission has delivered asteroid samples to Earth, and the largest asteroid sample return to date. Due to the pristine nature of these primitive carbonaceous-rich samples, they allow us a rare opportunity to study our solar system’s formation to a degree not previously possible. Isotopes of moderately volatile elements (MVEs) have been recently developed as robust tracers for tracking different volatilization events within our Solar System. Of the MVEs, K has gained significant interest in recent years due to its ideal chemical and physical properties combined with technique improvements. Due to this, several recent studies have investigated the K isotope systematics within bulk chondrites and found an isotopic dichotomy be-tween the carbonaceous and non-carbonaceous chondrites (Figure 1), likely reflecting their different reservoirs which formed in the inner and outer protoplanetary disk respectively. Furthermore, systematic K isotope variations across the different carbonaceous chondrite groups and correlations with mass independent isotope systems such as Cr, Ti, and Ni, have also been observed. As a result, establishing the K isotope composition of bulk Bennu can be used to test the mission hypothesis that “Bennu’s parent asteroid accreted in the outer protoplanetary disk, beyond Jupiter, as recorded by distinct isotopic anomalies in a variety of elements” . In addition to early solar system processes, K isotopes can also be fractionated by space weathering as shown by comparisons between mature and immature lunar regolith. The degree of K isotope fractionation caused by space weathering correlates with the regolith maturity index and is an order of magnitude larger than what is observed in bulk meteorite samples which have not undergone strong space weathering processes. As such, the K isotope systematics of Bennu samples could provide an independent assessment of regolith maturity of the asteroid surface, helping to directly test the mission hypothesis that “Space weathering changed the chemistry and mineralogy of optically active surfaces” . In order to test both this hypothesis, and the hypothesis relating to Bennu’s accretion location, we aim to conduct high-precision K isotope analysis on bulk Bennu aggregates.

K Wang↗
NASA NTRS1991

Test of the decaying dark matter hypothesis using the Hopkins Ultraviolet Telescope

Sciama's hypothesis that the dark matter associated with galaxies, galaxy clusters, and the intergalactic medium consists of tau neutrinos of rest mass 28-30 eV whose decay generates ultraviolet photons of energy roughly 14-15 eV, has been tested using the Hopkins Ultraviolet Telescope flows aboard the Space Shuttle Columbia. A straightforward application of Sciama's model predicts that a spectral line from neutrino decay photons should be observed from the rich galaxy cluster Abell 665 with an SNR of about 30. No such emission was detected. For neutrinos in the mass range 27.2-32.1 eV, the observations set a lower lifetime limit significantly greater than Sciama's model requires.

Davidsen, A. F.↗
NASA NTRS1996

Random root movements in weightlessness

The dynamics of root growth was studied in weightlessness. In the absence of the gravitropic reference direction during weightlessness, root movements could be controlled by spontaneous growth processes, without any corrective growth induced by the gravitropic system. If truly random of nature, the bending behavior should follow so-called 'random walk' mathematics during weightlessness. Predictions from this hypothesis were critically tested. In a Spacelab ESA-experiment, denoted RANDOM and carried out during the IML-2 Shuttle flight in July 1994, the growth of garden cress (Lepidium sativum) roots was followed by time lapse photography at 1-h intervals. The growth pattern was recorded for about 20 h. Root growth was significantly smaller in weightlessness as compared to gravity (control) conditions. It was found that the roots performed spontaneous movements in weightlessness. The average direction of deviation of the plants consistently stayed equal to zero, despite these spontaneous movements. The average squared deviation increased linearly with time as predicted theoretically (but only for 8-10 h). Autocorrelation calculations showed that bendings of the roots, as determined from the 1-h photographs, were uncorrelated after about a 2-h interval. It is concluded that random processes play an important role in root growth. Predictions from a random walk hypothesis as to the growth dynamics could explain parts of the growth patterns recorded. This test of the hypothesis required microgravity conditions as provided for in a space experiment.

STS-65 Shuttle Project↗
NASA NTRS1990

Testing for lightning as a source of radio bursts observed on the nightside of Venus

In certain previous studies of radio burst events recorded by the Pioneer Venus Orbiting Electric Field Detector (OEFD), data were sorted for statistical purposes according to occurrence at filter band frequencies smaller than or greater than typical values of the ambient electron gyrofrequency. The expectation in making this distinction was that the lowest frequency signals, at 100 Hz, were candidates for propagation through the ionosphere to the spacecraft in the whistler mode, and that the higher frequency signals, if of subionospheric origin, would require some different ionospheric penetration mechanism. On the basis of certain assumptions about the homogeneity and horizontal stratification of the Venusian nightside ionosphere, methods were developed for case-by-case testing of the hypothesis that any particular burst event originated in subionospheric lightning. The tests, which are capable of refinement, allow prediction of the resonance cone angle, refractive index, wave dispersion, and wave polarization. The tests have been applied to data from 11 periods along 7 orbits, and are believed to represent an improved way of categorizing OEFD burst data for purposes of investigating source/propagation mechanisms. Four of the five burst events that were not found consistent with the lightning hypothesis involved receptions at multiple OEFD filter band frequencies.

Sonwalkar, Vikas S.↗
NASA NTRS1984

Feige 24 - Wind/flare accretion by a hot DAZ1 degenerate

Model stellar envelopes have been constructed in order to represent the outer layers of the hot DAZ white dwarf component of the spectroscopic binary Feige 24. The results of the calculations show that the metals, discovered by Dupree and Raymond (1983) should diffuse rapidly into the interior unless a continuous source of these elements is present in the system. It is proposed that this source is a stellar wind from the red dwarf component of this detached close binary, and diffusion and accretion calculations are provided to support the hypothesis. Recent EXOSAT observations of Feige 24 provide additional evidence to test the accretion hypothesis. The accreting material may provide significant X-ray opacity for this object. This discussion is extended to other similar M dwarf-white dwarf close binary systems.

Sion, E. M.↗
NASA NTRS1975

Solar test of Dirac's large number hypothesis

An investigation is conducted regarding the implications of Dirac's theories (1973, 1974) concerning the creation of new matter. It is found that Dirac's theory of multiplicative creation, but not his theory of additive creation, is not in contradiction with known facts about the sun. According to the theory of additive creation, matter is formed uniformly throughout space. The concept of multiplicative creation implies that existing matter multiplies itself in proportion to the amount of matter already present.

Chin, C.-W.↗
NASA NTRS1980

Conditional sampling technique to test the applicability of the Taylor hypothesis for the large-scale coherent structures

Comparisons of the distributions of large scale structures in turbulent flow with distributions based on time dependent signals from stationary probes and the Taylor hypothesis are presented. The study investigated an area in the near field of a 7.62 cm circular air jet at a Re of 32,000, specifically having coherent structures through small-amplitude controlled excitation and stable vortex pairing in the jet column mode. Hot-wire and X-wire anemometry were employed to establish phase averaged spatial distributions of longitudinal and lateral velocities, coherent Reynolds stress and vorticity, background turbulent intensities, streamlines and pseudo-stream functions. The Taylor hypothesis was used to calculate spatial distributions of the phase-averaged properties, with results indicating that the usage of the local time-average velocity or streamwise velocity produces large distortions.

Hussain, A. K. M. F.↗
NASA NTRS1985

Spacelab life sciences flight experiments - An integrated approach to the study of cardiovascular deconditioning and orthostatic hypotension

A Spacelab experiment to study cardiovascular deconditioning and orthostatic hypotension is proposed. Previous experiments that have attempted to explain the factors which cause alterations in cardiovascular regulation are investigated. Based on these observations a hypothesis which states cephalad fluid shift produces cardiovascular changes is studied. The testing of the hypothesis is to include the analysis of physiological and anatomical changes in humans and animals for preflight and in-flight periods. The procedures and measurement techniques for the experiment are described.

Gaffney, F. A.↗
NASA NTRS1989

Shape matching utilizing indexed hypotheses generation and testing

An indexing mechanism is developed as part of an overall scheme called SMITH (shape matching utilizing indexed hypothesis generation and testing) for two-dimensional model-based object recognition. The approach is based on a dynamic programming implementation of attributed string matching, is computationally efficient, and works effectively for both nonoccluded and occluded shapes. Another advantage of this technique is that models may be inserted or deleted with relatively little cost.

Mehrotra, Rajiv↗
NASA NTRS2024

Bulk Major and Trace Elemental Composition of an Aggregate Sample From Asteroid Bennu

On September 24, 2023, NASA’s OSIRIS-REx mission returned a capsule to Earth carrying material from asteroid Bennu. This event was the first time a U.S. mission delivered pristine samples from an asteroid and is the largest asteroid sample return to date. As these samples represent some of the oldest, most primitive, and pristine materials available to us, and which originate from a known and well-studied asteroid, they allow us a rare opportunity to gain a better understanding of the formation and evolution of our solar system. Key to understanding the material returned from asteroid Bennu is establishing its bulk chemical composition. Previous studies have shown that each chondrite group has a distinct elemental composition. For the carbonaceous chondrites specifically, each group exhibits a distinct pattern of moderately and highly volatile elemental depletions, relative to CI chondrites. CI chondrites are considered the most primitive chondrite group and broadly represent the solar photosphere composition. The two most striking features of these depletion patterns is that the moderately volatile element depletions increase with decreasing 50% condensation temperature and then plateau out at abundances that roughly correlate with matrix abundance. Due to these distinctive patterns, bulk elemental composition has become an important classification tool for establishing the different chondrite groups and the connections between them. As such, determining the bulk chemical composition of the Bennu aggregates will help to test two mission hypothesis: “Bennu’s bulk elemental composition reflects that of its main parent asteroid and is similar to the composition of the Sun, with depletions in moderately to highly volatile elements” and “Bennu’s dominant lithologies are comparable in bulk mineralogy, petrology, and composition to the most aqueously altered carbonaceous chondrites” . Furthermore as the carbonaceous and non-carbonaceous chondrites are thought to have likely formed in the inner and outer protoplanetary disk, respectively (e.g., [8]), determining the bulk chemical compositions of the Bennu aggregates will also help test the major mission hypothesis that “Bennu's parent body formed beyond the snow line by accretion of material in the pro-toplanetary disk” . Regarding chondrite formation, observed elemental patterns have been successfully used to model how the mixing of volatile-rich and volatile-poor chondritic components can produce the observed carbonaceous chondrites groups. They have also been significant in investigating how volatilization processes influenced chondrite formation. As such, establishing bulk chemical compositions of the pristine Bennu samples is vital for understanding the asteroid, and solar system formation. To begin this processes, we analyzed the bulk major and trace elemental compositions of Bennu aggregates.

P Koefoed↗
NASA NTRS1988

A test of the massive binary black hole hypothesis - Arp 102B

The emission-line spectra of several AGN have broad peaks which are significantly displaced in velocity with respect to the host galaxy. An interpretation of this effect in terms of orbital motion of a binary black hole predicts periods of a few centuries. It is pointed out here that recent measurements of the masses and sizes of many low-luminosity AGN imply orbital periods much shorter than this. In particular, it is found that the elliptical galaxy Arp 102B is the most likely candidate for observation of radial velocity variations; its period is expected to be about 3 yr. The H-alpha line profile of Arp 102B has been measured for 5 yr without detecting any change in velocity, and it is thus found that a rather restrictive observational test of the massive binary black hole hypothesis already exists, albeit for this one object.

Helpern, J. P.↗