The experiments of Biosatellite II
Biosatellite 2 onboard experiments studying weightlessness effects on biological processes and interaction with radiation from Sr 85 gamma ray source
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Biosatellite 2 onboard experiments studying weightlessness effects on biological processes and interaction with radiation from Sr 85 gamma ray source
Radiotracking and monitoring of free-living animals in natural environments is providing an effective new technique for acquiring information on biological processes, including animal orientation and navigation. To test the practicability of extending the technique by using satellite systems for tracking animals, a female elk was instrumented with an electronic collar. It contained both the Interrogation Recording Location System (IRLS) transponder and a Craighead-Varney ground-tracking transmitter. The elk was successfully tracked and monitored by satellite during month of April 1970. This was the first time an animal had been tracked by satellite on the surface of the earth.
A program, designed specifically to study the respective effects of some common data problems on results obtained through stepwise iterative Fourier transformation of synthetic data with known waveform composition, was outlined. Included in this group were the problems of gaps in the data, different time-series lengths, periodic but nonsinusoidal waveforms, and noisy (low signal-to-noise) data. Results on sinusoidal data were also compared with results obtained on narrow band noise with similar characteristics. The findings showed that the analytic procedure under study can reliably reduce data in the nature of (1) sinusoids in noise, (2) asymmetric but periodic waves in noise, and (3) sinusoids in noise with substantial gaps in the data. The program was also able to analyze narrow-band noise well, but with increased interpretational problems. The procedure was shown to be a powerful technique for analysis of periodicities, in comparison with classical spectrum analysis techniques. However, informed use of the stepwise procedure nevertheless requires some background of knowledge concerning characteristics of the biological processes under study.
A preliminary progress report is presented for the Viking biological investigation through its first month. The carbon assimilation, gas exchange, and labeled release experiments are described in detail, and the chronology of the experiments is outlined. For the first experiment, it is found that a small amount of gas was converted into organic material in one sample and that heat treatment of a duplicate sample prevented such conversion. In the second experiment, a substantial amount of O2 was detected along with significant increases in CO2 and small changes in N2. In the third experiment, a significant amount of radioactive gas was evolved from one sample, but not from a duplicate heat-treated sample. Possible biological and nonbiological interpretations are considered for these results. It is concluded that while the experiments provide clear evidence for the occurrence of chemical reactions and while the results do not violate any prima facie criteria for biological processes, a definitive answer cannot yet be given to the question of whether life exists on Mars.
This experiment was developed in order to conduct engineering and operational tests of electrokinetic equipment in a micro-gravity environment. The experimental hardware in general functioned as planned and electrophoretic separations were obtained in space. The results indicated the development of satisfactory sample collection, return, and preservation techniques. The application of a near-zero zeta potential interior wall coating to the experimental columns, confirmation of biocompatibility of all appropriate hardware components, and use of a sterile operating environment provided a significant step forward in the development of a biological processing facility in space. A separation of a test of aldehyde-fixed rabbit, human, and horse red blood cells was obtained. Human kidney cells were separated into several components and viable cells returned to earth. The isotachophoretic separation of red cells was also demonstrated. Problems associated with the hardware led to a lack of success in the attempt to separate subpopulations of human lymphocytes.
Man's response to exposure to spaceflight and weightlessness is expressed in physiological adjustments which involve his health and ability to function. The amplitude and periodicity of fluctuations in biological processes affect various functions and responses to provocative stimuli. Primates and other species are subjected to tests to determine the consequences of an altered biorhythm on work and performance, emotional stability, biomedical evaluation in space, the ability to cope with the unexpected, and susceptibility to infection, toxicity, radiation, drugs, and stress. Factors in the environment or operational setup which can change the physiological baseline must be determined and controlled.
Radiative equilibrium and radiative convective temperature profiles for the earth's evolving atmosphere have been calculated. If the atmosphere evolved from one rich in carbon dioxide, and deficient in oxygen, to its present composition, the temperature structure showed considerable change. The models of 3 to 4 billion years ago display steadily decreasing temperatures with altitude, being 185 K at pressures associated with the present-day upper stratosphere. A lapse rate feature similar to the present-day tropopause is not indicated until about 1 billion years ago; but the stratospheric region is approximately 15 K colder than presently found at comparable pressures. Surface temperatures approximately 10 K warmer than at present existed until nearly 1 billion years ago. When the oxygen content exceeded roughly 0.1 times the present level, surface temperatures began to decrease. If biological processes are important to carbon dioxide - ozone variations, such as has been suggested during the Ice Ages, then estimates of surface temperature should include the effects of both gases.
To promote potential commercial applications of low-g technology, the materials processing and manufacturing in space program is structured to: (1) analyze the scientific principles of gravitational effects on processes used in producing materials; (2) apply the research toward the technology used to control production process (on Earth or in space, as appropriate); and (3) establish the legal and managerial framework for commercial ventures. Presently federally funded NASA research is described as well as agreements for privately funded commercial activity, and a proposed academic participation process. The future scope of the program and related capabilities using ground based facilities, aircraft, sounding rockets, and space shuttles are discussed. Areas of interest described include crystal growth; solidification of metals and alloys; containerless processing; fluids and chemical processes (including biological separation processes); and processing extraterrestrial materials.
Concurrent surface water measurements made from a moving oceanographic research vessel were used to calibrate and interpret remotely sensed data collected over a plume in the New York Bight Apex on June 23, 1977. Multiple regression techniques were used to develop equations to subsequently map synoptic distributions of water quality parameters, chlorophyll A and total suspended matter, in the remotely sensed scene. Thermal (which did not have surface calibration values) and water quality parameter distributions indicated a cold mass of upwelled water in the Bight Apex with an overflowing nutrient-rich warm water plume that originated in the Sandy Hook Bay and flowed south near the New Jersey shoreline. Additional comparison of remotely sensed thermal and optical properties of the water with shipboard measurements indicated that remotely sensed data may be particularly useful for studying physical and biological processes in the top several meters of surface water at the plume boundaries.
Concurrent surface water measurements made from a moving oceanographic research vessel were used to calibrate and interpret remotely sensed data collected over a plume in the New York Bight Apex on 23 June 1977. Multiple regression techniques were used to develop equations to map synoptic distributions of chlorophyll a and total suspended matter in the remotely sensed scene. Thermal (which did not have surface calibration values) and water quality parameter distributions indicated a cold mass of water in the Bight Apex with an overflowing nutrient-rich warm water plume that originated in the Sandy Hook Bay and flowed south near the New Jersey shoreline. Data analysis indicates that remotely sensed data may be particularly useful for studying physical and biological processes in the top several metres of surface water at plume boundaries.
NASA's Global Biology Research Program and its goals for greater understanding of planetary biological processes are discussed. Consideration is given to assessing major pathways and rates of exchange of elements such as carbon and nitrogen, extrapolating local rates of anaerobic activities, determining exchange rates of ocean nutrients, and developing models for the global cycles of carbon, nitrogen, sulfur, and phosphorus. Satellites and sensors operating today are covered: the Nimbus, NOAA, and Landsat series. Block diagrams of the software and hardware for a typical ground data processing and analysis system are provided. Samples of the surface cover data achieved with the Advanced Very High Resolution Radiometer, the Multispectral Scanner, and the Thematic Mapper are presented, as well as a productive capacity model for coastal wetlands. Finally, attention is given to future goals, their engineering requirements, and the necessary data analysis system.
Laboratory tests were performed to quantify the expected NO(x) production by lightning and biological processes. Attention was focused on energy deposition by lightning and the oxygen partial pressure of soil, and one-dimensional photochemical models were defined for the tropospheric distributions of NO and HNO3 for various NO source strengths. The Lightning Facility data were compared with air samples of N2O production gathered during over 2 yr of flights through storms. Soil NO(x) productions were studied in terms of nitrification processes involving Nitrosomonas europaea and Alcaligenes faecalis bacteria, which change ammonium to nitrite and release NO and N2O as byproducts. The results indicate that atmospheric NO(x) is generated at two tropospheric levels, with lightning and soil bacteria being significant contributors.
Numerous studies have been concerned with the possibility of a reduction of the stratospheric ozone layer. Such a reduction could lead to an enhanced penetration of ultraviolet (UV) radiation to the ground, and, as a result, to damage in the case of several biological processes. It is pointed out that the distributions of many trace gases, such as ozone, are governed in part by transport processes. The present investigation presents a two-dimensional photochemistry-transport model using the residual circulation. The global distribution of both ozone and components with ground sources computed in this model is in good agreement with the observations even though slow diffusion is adopted. The agreement is particularly good in the Northern Hemisphere. The results provide additional support for the idea that tracer transport in the stratosphere is mainly of advective nature.
Knowledge of the physics of the scattering behavior of vegetation will ultimately serve the remote sensing and earth science community in many ways. For example, it will provide: (1) insight and guidance in developing new extraction techniques of canopy characteristics, (2) a basis for better interpretation of off-nadir satellite and aircraft data, (3) a basis for defining specifications of future earth observing sensor systems, and (4) a basis for defining important aspects of physical and biological processes of the plant system. The overall objective of the three-year study is to improve our fundamental understanding of the dynamics of directional scattering properties of vegetation canopies through analysis of field data and model simulation data. The specific objectives are to: (1) collect directional reflectance data covering the entire exitance hemisphere for several common vegetation canopies with various geometric structure (both homogeneous and row crop structures), (2) develop a scene radiation model with a general mathematical framework which will treat 3-D variability in heterogeneous scenes and account for 3-D radiant interactions within the scene, (3) conduct validations of the model on collected data sets, and (4) test and expand proposed physical scattering mechanisms involved in reflectance distribution dynamics by analyzing both field and modeling data.
The purpose was to determine the optimal use by wheat plants of the N sources expected from processing biological waste products, NO3(-),NO2(-)NH4(+), and urea. The approach was to determine the uptake and metabolic products of each N source (from single and multiple component solutions), inhibitory effects of each, feedback inhibition, and overall in vivo regulation of the rates of assimilation of each by wheat plants. Previously, researchers determined the interactions of NO3(-),NO2(-),NH4(+) on each other's uptake and incorporation. The assimilation and some of its effects on NO3(-) and NH4(+) assimilation which have been completed to data are discussed.
Some of the factors are outlined that leads one to endorse the concept of focused, international geosphere-biosphere program, whose goal is to understand the interactive physical, chemical, and biological processes that regulate the Earth's unique environment for life, the changes that are occurring in this system, and the manner in which they are influenced by human actions. There is a pressing need to assess the consequence of human activities in the context of natural global change and to provide the body of knowledge necessary to chart a wise course to the future. A number of specific objectives were developed which leads to the conclusion that the need for new programs of observation of the Earth as a planet, a better understanding of the interactive processes that governs its changes, the development of a new generation of coupled modes, and the design of suitable tests to guide the development of these models and the understanding of the processes involved. Some general recommendations are summarized.
All organisms require energy in a chemical form for maintenance and growth. In contemporary life this chemical energy is obtained by the synthesis of the phosphoanhydride bonds of ATP. Among the biological processes that yield ATP, fermentation is generally considered primitive, because it operates under anaerobic conditions by substrate-level phosphorylation which does not require compartmentation by membranes. Fermentation by the glycolytic pathway, which is found in almost every living cell, is an especially attractive energy source for primitive life. Glycolysis not only produces useful chemical energy (ATP), but intermediates of this pathway are also involved in amino acid synthesis and photosynthetic carbon-fixation. It is believed that energy and substrates needed for the origin of life were provided by nonenzymatic chemical reactions that resemble the enzyme-mediated reactions of glycolysis. These nonenzymatic reactions would have provided a starting point for the evolutionary development of glycolysis.
A review of the metabolic support systems used and the metabolic support requirements provided on past and current spaceflight programs is presented. This review will provide familiarization with unique constraints of space flight and technology as it relates to inflight metabolic support of astronauts. This information, along with a general review of the NASA effort to develop a Controlled Ecological Life Support System (CELSS) will define the general scenario of metabolic support for a lunar base. A phased program of metabolic support for a lunar base will be elucidated. Included will be discussion of the CELSS water reclamation and food recycling technology as it now exists and how it could be expected to be progressively incorporated into the lunar base. This transition would be from a relatively open system in the initial development period, when mechanical phase change water reclamation and minimal plant growth are incorporated, to the final period when practically total closure of the life support system will be proved through physicochemical and biological processes. Finally, a review of the estimated metabolic intake requirements for the occupants of a lunar base will be presented.