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At least 703 records · Page 39

Preliminary investigations into solutal flow about growing tetragonal lysozyme crystals

A series of preliminary experiments were done to investigate solutal flow about growing lysozyme crystals and its effects. Density-gradient-driven flow was observed using a schlieren optical system. Crystals used ranged from 0.3 to 1.72 mm across the (110) face, and protein concentrations were from 3.7 to 23.7 mg/ml. The convective plume velocities were found to be from 10 to 50 microns/s, which correlated with those predicted to occur based upon a diffusive-convective model. When microcrystals of lysozyme, less than 20 microns across the (110) face were subjected to directed solution flows, the growth rate was found to rapidly decrease over the 8-20 h course of the experiment. Solution flow rates used ranged from 18 to 40 microns/s, and protein concentrations were from 7.3 to 11.7 mg/ml, conditions typical of larger (greater than 0.5 mm) crystals in the terminal phases of a typical crystal growth procedure.

Pusey, Marc↗

Comparison of electrical and optical characteristics in gas-phase and gas-liquid phase discharges

This paper presents an AC-excited argon discharge generated using a gas-liquid (two-phase) hybrid plasma reactor, which mainly consists of a powered needle electrode enclosed in a conical quartz tube and grounded deionized water electrode. The discharges in the gas-phase, as well as in the two-phase, exhibit two discharge modes, i.e., the low current glow-like diffuse mode and the high current streamer-like constrict mode, with a mode transition, which exhibits a negative resistance of the discharges. The optical emission spectral analysis shows that the stronger diffusion of the water vapor into the discharge region in the two-phase discharges boosts up the generation of OH (A–X) radicals, and consequently, leads to a higher rotational temperature in the water-phase plasma plume than that of the gas-phase discharges. Both the increase of the power input and the decrease of the argon flow rate result in the increase of the rotational temperature in the plasma plume of the water-phase discharge. The stable two-phase discharges with a long plasma plume in the water-phase under a low power input and gas flow rate may show a promising prospect for the degradation of organic pollutants, e.g., printing and dyeing wastewater, in the field of environmental protection.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

High-Temperature Optical Window Design

A high-temperature optical window is essential to the optical diagnostics of high-temperature combustion rigs. Laser Doppler velocimetry, schlieren photography, light sheet visualization, and laser-induced fluorescence spectroscopy are a few of the tests that require optically clear access to the combustor flow stream. A design was developed for a high-temperature window that could withstand the severe environment of the NASA Lewis 3200 F Lean Premixed Prevaporized (LPP) Flame Tube Test Rig. The development of this design was both time consuming and costly. This report documents the design process and the lessons learned, in an effort to reduce the cost of developing future designs for high-temperature optical windows.

Roeloffs, Norman↗

Two-Photon Flow Cytometry

Flow cytometry is a powerful technique for obtaining quantitative information from fluorescence in cells. Quantitation is achieved by assuring a high degree of uniformity in the optical excitation and detection, generally by using a highly controlled flow such as is obtained via hydrodynamic focusing. In this work, we demonstrate a two-beam, two- channel detection and two-photon excitation flow cytometry (T(sup 3)FC) system that enables multi-dye analysis to be performed very simply, with greatly relaxed requirements on the fluid flow. Two-photon excitation using a femtosecond near-infrared (NIR) laser has the advantages that it enables simultaneous excitation of multiple dyes and achieves very high signal-to-noise ratio through simplified filtering and fluorescence background reduction. By matching the excitation volume to the size of a cell, single-cell detection is ensured. Labeling of cells by targeted nanoparticles with multiple fluorophores enables normalization of the fluorescence signal and thus ratiometric measurements under nonuniform excitation. Quantitative size measurements can also be done even under conditions of nonuniform flow via a two-beam layout. This innovative detection scheme not only considerably simplifies the fluid flow system and the excitation and collection optics, it opens the way to quantitative cytometry in simple and compact microfluidics systems, or in vivo. Real-time detection of fluorescent microbeads in the vasculature of mouse ear demonstrates the ability to do flow cytometry in vivo. The conditions required to perform quantitative in vivo cytometry on labeled cells will be presented.

Zhog, Cheng Frank↗

Turbulence control on airborne laser platform

An avctive flow control device to generate large-scale, periodic structures in a turbulent shear flow is developed. Together with adaptive optics, the device may be used on airborne laser platforms to reduce or eliminate optical distortion caused by the turbulence in the aircraft's boundary layer. A flat plate towed in a water channel is used as a test bed. A cyclic jet issuing from a spanwise slot is used to collect the turbulent boundary layer for a finite time during its 'on' period. When the jet is turned 'off', all of the turbulent fluid is released instantaneously in one large eddy that convects downstream. Flow visualization and hot-film probe measurements are used together with pattern recognition algorithms to demonstrate the viability of the flow control method. The instantaneous velocity signal is used to compute important statistical quantities of the random velocity field, such as the mean, the root-mean-square, the spectral distribution, and the probability density function. When optimized for a given boundary layer, the cyclic jet produces periodic structures that are qualitatively similar to the random, naturally occurring ones. These structures seem to trigger the onset of bursting events near the wall. Thus, the present device generates periodic structures in both the outer and inner regions of a turbulent boundary layer.

Gad-El-hak, Mohamed↗

Cellulose nanofibrils and nanocrystals in confined flow: Single-particle dynamics to collective alignment revealed through scanning small-angle x-ray scattering and numerical simulations

Nanostructured materials made through flow-assisted assembly of proteinaceous or polymeric nanosized fibrillar building blocks are promising contenders for a family of high-performance biocompatible materials in a wide variety of applications. Optimization of these processes relies on improving our knowledge of the physical mechanisms from nano- to macroscale and especially understanding the alignment of elongated nanoparticles in flows. Here, we study the full projected orientation distributions of cellulose nanocrystals (CNCs) and nanofibrils (CNFs) in confined flow using scanning microbeam SAXS. For CNCs, we further compare with a simulated system of dilute Brownian ellipsoids, which agrees well at dilute concentrations. However, increasing CNC concentration to a semidilute regime results in locally arranged domains called tactoids, which aid in aligning the CNC at low shear rates, but limit alignment at higher rates. Similarly, shear alignment of CNF at semidilute conditions is also limited owing to probable bundle or flock formation of the highly entangled nanofibrils. Finally, this work provides a quantitative comparison of full projected orientation distributions of elongated nanoparticles in confined flow and provides an important stepping stone towards predicting and controlling processes to create nanostructured materials on an industrial scale.

36 MATERIALS SCIENCE↗

Optical scanning system for laser velocimeter

An optical system was developed to provide fast incremental scanning of a backscattered laser velocimeter focus point over a 36-cm distance. The system is used to measure flow velocities at 16 positions along its optical axis and to scan these 16 positions up to 30 times a second. Dwell time at each location is approximately 2 milliseconds. Sample volumes typically are 0.2 mm in diameter by 1.4 cm in length. The optical scanning system consists of a wheel containing plane parallel quartz windows of various thicknesses. The laser velocimeter beams are imaged to a primary focus within the dead airspace of an optical cell. The beams emerging from the cell pass through the windows of the scanning wheel. The refraction of the beams passing through the windows causes an apparent shift of the focus within the optical cell and hence in the test zone. Light scattered from the secondary focus within the test zone is concurrently collected and reimaged through the same optical path which originally projected the primary focus. The reimaged backscattered light containing the velocity information is then collected and focused onto a photomultiplier detector system to complete the scanned laser velocimeter optical system.

Rhodes, D. B.↗

Using the ROSS optical streak camera as a tool to understand laboratory experiments of laser-driven magnetized shock waves

Supersonic flows with high Mach number are ubiquitous in astrophysics. High-powered lasers also have the ability to drive high Mach number, radiating shock waves in laboratory plasmas, and recent experiments along these lines have made it possible to recreate analogs of high Mach-number astrophysical flows under controlled conditions. Streak cameras such as the Rochester optical streak system (ROSS) are particularly helpful in diagnosing such experiments, because they acquire spatially resolved measurements of the radiating gas continuously over a large time interval, making it easy to observe how any shock waves and ablation fronts present in the system evolve with time. This paper summarizes new ROSS observations of a laboratory analog of the collision of a stellar wind with an ablating planetary atmosphere embedded within a magnetosphere. We find good agreement between the observed ROSS data and numerical models obtained with the FLASH code, but only when the effects of optical depth are properly taken into account.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Polarization (ellipsometric) measurements of liquid condensate deposition and evaporation rates and dew points in flowing salt/ash-containing combustion gases

An application of an optical polarization technique in a combustion environment is demonstrated by following, in real-time, growth rates of boric oxide condensate on heated platinum ribbons exposed to seeded propane-air combustion gases. The results obtained agree with the results of earlier interference measurements and also with theoretical chemical vapor deposition predictions. In comparison with the interference method, the polarization technique places less stringent requirements on surface quality, which may justify the added optical components needed for such measurements.

Seshadri, K.↗

Near-wall, three-dimensional turbulence measurements: A challenge for laser velocimetry

A new laser velocimeter approach is presented, which has distinct advantages in near-wall, two- and three-dimensional turbulence measurement applications. The approach does require placing a probe into the flow; but in return, there are some important benefits, such as, the direct measurement of the crossflow velocity, w, at a grazing incidence, and the ability to size optical components for the scale of the flow rather than the size of the facility. Promising resuls were obtained with this approach for a two-dimensional turbulent boundary layer.

Johnson, D. A.↗

Novel Optical Technique Developed and Tested for Measuring Two-Point Velocity Correlations in Turbulent Flows

A novel technique for characterizing turbulent flows was developed and tested at the NASA Glenn Research Center. The work is being done in collaboration with the University of Pittsburgh, through a grant from the NASA Microgravity Fluid Physics Program. The technique we are using, Homodyne Correlation Spectroscopy (HCS), is a laser-light-scattering technique that measures the Doppler frequency shift of light scattered from microscopic particles in the fluid flow. Whereas Laser Doppler Velocimetry gives a local (single-point) measurement of the fluid velocity, the HCS technique measures correlations between fluid velocities at two separate points in the flow at the same instant of time. Velocity correlations in the flow field are of fundamental interest to turbulence researchers and are of practical importance in many engineering applications, such as aeronautics.

Zimmerli, Gregory A.↗

Diagnosing collisions of magnetized, high energy density plasma flows using a combination of collective Thomson scattering, Faraday rotation, and interferometry (invited)

A suite of laser based diagnostics is used to study interactions of magnetised, supersonic, radiatively cooled plasma flows produced using the Magpie pulse power generator (1.4 MA, 240 ns rise time). Collective optical Thomson scattering measures the time-resolved local flow velocity and temperature across 7–14 spatial positions. The scattering spectrum is recorded from multiple directions, allowing more accurate reconstruction of the flow velocity vectors. The areal electron density is measured using 2D interferometry; optimisation and analysis are discussed. The Faraday rotation diagnostic, operating at 1053 nm, measures the magnetic field distribution in the plasma. Measurements obtained simultaneously by these diagnostics are used to constrain analysis, increasing the accuracy of interpretation.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

A high-speed photographic system for flow visualization in a steam turbine

A photographic system was designed to visualize the moisture flow in a steam turbine. Good performance of the system was verified using dry turbine mockups in which an aerosol spray simulated, in a rough way, the moisture flow in the turbine. Borescopes and fiber-optic light tubes were selected as the general instrumentation approach. High speed motion-picture photographs of the liquid flow over the stator blade surfaces were taken using stroboscopic lighting. Good visualization of the liquid flow was obtained. Still photographs of drops in flight were made using short duration flash sources. Drops with diameters as small as 30 micrometers (0.0012 in.) could be resolved. In addition, motion pictures of a spray of water simulating the spray off the rotor blades and shrouds were taken at normal framing rates. Specially constructed light tubes containing small tungsten-halogen lamps were used. Sixteen millimeter photography was used in all cases. Two potential problems resulting from the two-phase turbine flow (attenuation and scattering of light by the fog present and liquid accumulation on the borescope mirrors) were taken into account in the photographic system design but not evaluated experimentally.

Barna, G. J.↗

Dynamical effects of annihilation in pair-dominated winds

The steady, spherically symmetric flow of an ideal fluid dominated by photons and ultrarelativistic electron-positron pairs is analyzed. A new wind equation and a set of critical point conditions are obtained which describe the relativistic flow of an annihilation gas in which the flow velocity exceeds the diffusion velocity of the photons. Numerical results are reported which suggest the possible existence of trapped, pure-pair winds driven by the combined pressure of the pairs and the photons. Most of the annihilation occurs below the critical radius in trapped flows, and a substantial fraction of the total energy of the injected pairs is converted into kinetic energy and radiation. Accurate numerical solutions for the flow velocity and the positron loss rate in optically thin, Newtonian winds are obtained, and a useful approximate analytic relation between the positron loss rate and the flow velocity is derived which suggests that a large number of pairs may survive the annihilation region, ultimately escaping the potential well.

Becker, Peter A.↗

Simultaneous measurements of velocity and pressure fields in subsonic and supersonic flows through image-intensified detection of laser-induced fluorescence

An optical technique is presented for combined, spatially resolved measurements of two-dimensional velocity and pressure fields in compressible flows. The single-mode frequency of an argon laser is fixed in the wing of an absorption line of iodine molecules, seeded in an underexpanded round jet of nitrogen gas. The emitted fluorescence, being proportional to the amount of absorbed radiation and hence the absorption line-shape function, is detected with an intensified 100 x 100 photodiode array camera. A single-microchannel-plate image intensifier is fiber-optically coupled to the array in order to improve time resolution and SNR. Three components of the velocity vector in a cross-sectional plane are sequentially probed with four laser sheets from three different directions. By shifting the laser frequency in one of the sheets with a piezo-tuned intra-cavity etalon, the slope of the absorption line can be measured in situ in order to provide the required scaling factor for the velocity measurement. With its short measurement times of less than 250 ms, this method is well suited for blow-down wind tunnel experiments.

Hiller, B.↗

X-ray spectra of clusters of galaxies

The X-ray emission from luminous clusters of galaxies is dominated by thermal bremsstrahlung from an intergalactic medium. The central density of the gas is strongly correlated with the X-ray surface brightness. The X-ray surface brightness S(sigma) of many clusters is well modeled by a law of the form S(sigma) alpha S(o) 1 + R(2)/A(2) to the minus 3 beta + 1/2 power with beta approximately equal to 0.66. However, this model does not fit the X-ray spectral or optical galaxy counts well. In clusters with cooling flows in their center there is a strong correlation between the cooling rates of X-ray emitting material and optical H alpha emission. It is not clear, at present, what percentage of the virial mass of the cluster is in hot gas but if beta = 0.66, it is possible for the values to be of the order of 1/2. Spatially resoled X-ray spectroscopy is necessary to determine this value with any accuracy.

Mushotzky, R. F.↗

Effects of Lewis Number on Temperatures of Spherical Diffusion Flames

Spherical diffusion flames supported on a porous sphere were studied numerically and experimentally. Experiments were performed in 2.2 s and 5.2 s microgravity facilities. Numerical results were obtained from a Chemkin-based program. The program simulates flow from a porous sphere into a quiescent environment, yields both steady-state and transient results, and accounts for optically thick gas-phase radiation. The low flow velocities and long residence times in these diffusion flames lead to enhanced radiative and diffusive effects. Despite similar adiabatic flame temperatures, the measured and predicted temperatures varied by as much as 700 K. The temperature reduction correlates with flame size but characteristic flow times and, importantly, Lewis number also influence temperature. The numerical results show that the ambient gas Lewis number would have a strong effect on flame temperature if the flames were steady and nonradiating. For example, a 10% decrease in Lewis number would increase the steady-state flame temperature by 200 K. However, for these transient, radiating flames the effect of Lewis number is small. Transient predictions of flame sizes are larger than those observed in microgravity experiments. Close agreement could not be obtained without either increasing the model s thermal and mass diffusion properties by 30% or reducing mass flow rate by 25%.

Santa, K. J.↗

High-Fidelity Velocity and Concentration Measurements of Turbulent Buoyant Jets

Accurate models of turbulent buoyant flows are essential for the design of the cooling circuit of nuclear reactors and passive safety systems. However, available models fail to fully capture the physics of turbulent mixing when buoyancy becomes predominant with respect to momentum. Therefore, high-fidelity experiments of well-controlled fundamental flows are needed to develop and validate more accurate models. We analyze experiments of positive and negative turbulent buoyant jets, both in uniform and stratified environments, with the aim of understanding the thermal hydraulics of turbulent mixing with variable density and providing high-fidelity data for the development and validation of turbulence models. Non-intrusive, simultaneous particle image velocimetry and laser-induced fluorescence measurements were carried out to acquire instantaneous velocity and concentration fields on a vertical section parallel to the axis of a jet in the self-similar region. The refractive index matching method was applied to measure high-resolution buoyant jets with up to 8.6% density difference. These data are free of the typical errors that characterize optical measurements of buoyancy-driven flows (e.g. natural and mixed convection) where the refractive index of the fluid is inhomogeneous throughout the measurement domain. Turbulent statistics and entrainment of buoyant jets in uniform and stratified environments are presented. These data are compared with non-buoyant jets in a uniform environment, as a reference to investigate the effects of buoyancy and stratification on turbulent mixing. The results will be used for the assessment of current turbulence models and as a basis for the development of a new model that captures turbulent mixing.

laser induced fluorescence↗