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Results for “Effective diffusivity”
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Determining the domain-level reaction-diffusion properties of an actin-binding protein transgelin-2 within cells
Proteins in cells undergo repeated binding to other molecules, thereby reducing the apparent extent of their intracellular diffusion. While much effort has been made to analytically decouple these combined effects of pure diffusion and chemical binding, it is difficult with conventional approaches to attribute the measured quantities to the nature of specific domains of the proteins. Motivated by the common goal in cell signaling research aimed at identifying the domains responsible for particular intermolecular interactions, here we describe a framework for determining the local physicochemical properties of cellular proteins associated with immobile scaffolds. To validate this new approach, we apply it to transgelin-2, an actin-binding protein whose intracellular dynamics remains elusive. We develop a fluorescence recovery after photobleaching (FRAP)-based framework, in which comprehensive combinations of domain-deletion mutants are created, and the difference among them in FRAP response is analyzed. We demonstrate that transgelin-2 in actin stress fibers (SFs) interacts with F-actin via two separate domains, and the chemical properties are determined for the individual domains. Its pure diffusion properties independent of the association to F-actin is also obtained. Our approach will thus be useful, as presented here for transgelin-2, in addressing the signaling mechanism of cellular proteins associated with SFs.
Hydrogen Diffusion In Oversaturated EPDM Rubber: The Effect Of Induced Strain
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Hydrogen Diffusion in Oversaturated EPDM Rubber: The Effect of Induced Strain
Computational study of hydrogen gas in EPDM rubber
Multimodel simulation of vertical gas transfer in a temperate lake
In recent decades, several lake models of varying complexity have been developed and incorporated in numerical weather prediction systems and climate models. To foster enhanced forecasting ability and verification, improvement of these lake models remains essential. This especially applies to the limited simulation capabilities of biogeochemical processes in lakes and greenhouse gas exchanges with the atmosphere. Here we present multi-model simulations of physical variables and dissolved gas dynamics in a temperate lake (Harp Lake, Canada). The five models (ALBM, FLake, LAKE, LAKEoneD, MTCR-1) considered within this most recent round of the Lake Model Intercomparison Project (LakeMIP) all captured the seasonal temperature variability well. In contrast, none of the models is able to reproduce the exact dates of ice-cover and ice-off, leading to considerable errors in the simulation of eddy diffusivity around those dates.We then conducted an additional modeling experiment with a diffusing passive tracer to isolate the effect of the eddy diffusivity on gas concentration. Remarkably, sophisticated k -" models do not demonstrate a significant difference in the vertical diffusion of a passive tracer compared to models with much simpler turbulence closures. All models simulate less intensive spring overturn compared to autumn. Reduced mixing in the models consequently leads to the accumulation of the passive tracer distribution in the water column. The lake models with a comprehensive biogeochemical module, such as ALBM and LAKE, predict dissolved oxygen dynamics adequate to the observed data. However, for the surface carbon dioxide concentration the correlation between modeled (ALBM, LAKE) and observed data is weak (~0.3). Overall our results indicate the need to improve the representation of physical and biogeochemical processes in lake models, thereby contributing to enhanced weather prediction and climate projection capabilities.
The effect of ''drift'' waves on plasma diffusion in a magnetic field vliyaniye ''dreyfovykh'' voln na diffuziyu plazmy v magnitnom pole
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Effect of shell splitting on radial diffusion in the magnetosphere.
Radial diffusion coefficient calculation in presence of magnetic shell splitting in magnetosphere
Effects of oxygen environment and surface diffused coatings on fatigue crack development in copper single crystals.
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A simplified stratified lake model for determining effects of wind variation and eddy diffusivity
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Cosmic ray scintillations. IV - The effects of non-field-aligned diffusion
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Cellular interface morphologies in directional solidification. III - The effects of heat transfer and solid diffusivity
The shape and stability of two-dimensional finite-amplitude cellular interfaces arising during directional solidification are compared for several solidification models that account differently for latent heat released at the interface, unequal thermal conductivities of melt and solid, and solute diffusivity in the solid. Finite-element analysis and computer-implemented perturbation methods are used to analyze the families of steadily growing cellular forms that evolve from the planar state. In all models a secondary bifurcation between different families of finite-amplitude cells exists that halves the spatial wavelength of the stable interface. The quantitative location of this transition is very dependent on the details of the model. Large amounts of solute diffusion in the solid retard the growth of large-amplitude cells.
Tidal evolution of globular clusters. II - The effects of Galactic tidal field and diffusion
The tidal evolution of globular clusters subject to various degrees of the internal diffusion process is investigated. In cases of negligible diffusion, clusters are found to be tidally truncated to the theoretical tidal radius at perigalacticon. There is no apparent orbital phase dependence of the tidal radius for clusters with eccentric orbits. In clusters with moderately short two-body relaxation time scales, diffusion processes significantly modify the structure of the outer regions in such a way that the limiting radius may be comparable to the tidal radius at apogalacticon. The Galactical tidal torque induces isotropy in the velocity dispersion of the outer regions of the cluster. For relaxed clusters, the velocity dispersion may be isotropic in the core, anisotropic in the envelope and isotropic near the limiting radius. Disk shocking is also very efficient for isotropizing the orbits of stars in the outer cluster regions. Stars with direct orbits are less stable, so that prolonged tidal interaction can lead to apparent retrograde rotation in the outer regions of the cluster.
Stray light effects on calibrations using a solar diffuser
The sun is the intended source of radiation for a solar diffuser for the calibration of spacecraft radiometric sensors. Two unwanted sources have also been identified: (1) solar radiation reflected from the earth and scattered by the atmosphere, and (2) solar radiation reflected from surrounding spacecraft structures. This paper describes the determination of the stray radiation incident on the diffuser for the High Resolution Imaging Spectrometer (HIRIS). Finally, the optimization of the calibration geometry is considered.
The effect of initial rate of subsonic diffusion on the stable subcritical mass-flow range of a conical shock diffuser
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Numerical Modeling of HgCdTe Solidification: Effects of Phase Diagram, Double-Diffusion Convection and Microgravity Level
Melt convection, along with species diffusion and segregation on the solidification interface are the primary factors responsible for species redistribution during HgCdTe crystal growth from the melt. As no direct information about convection velocity is available, numerical modeling is a logical approach to estimate convection. Furthermore influence of microgravity level, double-diffusion and material properties should be taken into account. In the present study, HgCdTe is considered as a binary alloy with melting temperature available from a phase diagram. The numerical model of convection and solidification of binary alloy is based on the general equations of heat and mass transfer in two-dimensional region. Mathematical modeling of binary alloy solidification is still a challenging numericial problem. A Rigorous mathematical approach to this problem is available only when convection is not considered at all. The proposed numerical model was developed using the finite element code FIDAP. In the present study, the numerical model is used to consider thermal, solutal convection and a double diffusion source of mass transport.
Buoyancy Effects in Fully-Modulated, Turbulent Diffusion Flames
Pulsed combustion appears to have the potential to provide for rapid fuel/air mixing, compact and economical combustors, and reduced exhaust emissions. The objective of this experiment (PuFF, for Pulsed-Fully Flames) is to increase the fundamental understanding of the fuel/air mixing and combustion behavior of pulsed, turbulent diffusion flames by conducting experiments in microgravity. In this research the fuel jet is fully-modulated (i.e., completely shut off between pulses) by an externally controlled valve system. This gives rise to drastic modification of the combustion and flow characteristics of flames, leading to enhanced fuel/air mixing compared to acoustically excited or partially-modulated jets. Normal-gravity experiments suggest that the fully-modulated technique also has the potential for producing turbulent jet flames significantly more compact than steady flames with no increase in exhaust emissions. The technique also simplifies the combustion process by avoiding the acoustic forcing generally present in pulsed combustors. Fundamental issues addressed in this experiment include the impact of buoyancy on the structure and flame length, temperatures, radiation, and emissions of fully-modulated flames.
Buoyancy Effects in Strongly-pulsed, Turbulent Diffusion Flames
The objective of this experiment is to better understand the combustion behavior of pulsed, turbulent diffusion flames by conducting experiments in microgravity. The fuel jet is fully-modulated (i.e., completely shut off between pulses) by an externally controlled valve system leading to enhanced fuel/air mixing compared to acoustically excited or partially-modulated jets. Experiments are conducted both in laboratories at UW and WPI and in the GRC 2.2s Drop Tower. A single fuel nozzle with diameter d = 2 mm is centered in a combustor 20 x 20 cm in cross section and 67 cm in height. The gaseous fuel flow (ethylene or a 50/50 ethylene/nitrogen mixture by volume) is fully-modulated by a fast-response solenoid valve with injection times from tau = 4 to tau = 300 ms. The nominal Reynolds number based on the fuel velocity during injection, U(sub jet), is 5,000. A slow oxidizer co-flow properly ventilates the flame and an electrically heated wire loop serves as a continuous ignition source. Diagnostic techniques include video imaging, fine-wire thermocouples and thermopile radiometers, and gas sampling and standard emissions instruments (the last in the laboratory only).