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At least 703 records · Page 39

Effects of sampling techniques on short-term survival and genotyping success of salmonid fry

ABSTRACT Objective Genetics tools have become an integral part of managing and understanding fish populations. Generally, a small tissue sample, such as a fin clip, is taken and then genotyped, with little effect on survival of the fish. However, tissue sampling may have a larger effect on juvenile fish survival compared to their adult counterparts. We evaluated survival and genotyping success of various genetic sampling techniques for Chinook Salmon Oncorhynchus tshawytscha and Rainbow Trout Oncorhynchus mykiss fry. Methods Three sampling treatments were evaluated including control (anesthetized and handled), fin clipping (partial caudal fin clip), and swabbing (OmniSwab was used to collect external mucus). Survival was monitored for 12 d posttreatment, and genotyping success was evaluated. Results Survival was high in all treatment groups (i.e., 0.93–1.00) but, on average, was lower in the swab treatment group. Genotyping was successful in 100% of the fin clip samples and 11–50% of the swab samples. Conclusions Results of this study suggest that sampling caudal-fin tissue does not negatively affect fry short-term survival and the small tissue samples yield highly successful genotyping results. Swabbing did not produce successful genotyping results, and fish sampled with swabs experienced higher mortality than those that received fin clips. Results indicate that fin clips should be used for collection of genetic samples from fry.

McCarrick, Darcy K.↗

Mechanism of the wurtzite to rocksalt phase transformation in cadmium sulfide single crystals shock compressed along the 𝑐-axis to elastic impact stresses of ∼ 5 GPa

Cadmium sulfide (CdS), which exhibits a wurzite (WZ) to rocksalt (RS) phase transformation at elevated stresses, is an ideal system to address the role of deformation on phase transformation mechanisms and kinetics. CdS has been shown to have very different elastic-inelastic behavior for shock propagation along the WZ 𝑐- and 𝑎-axes, and also exhibits significant differences in the time scale for the phase transformation when shocked along these different axes. As an important first step in examining the role of deformation in shock-induced phase transformations, here we present in situ, time-resolved x-ray diffraction (XRD) measurements in single-event, shock wave experiments on CdS single crystals shocked along the WZ 𝑐-axis to elastic impact stresses near or above ∼ 5 GPa, where a marked increase in the kinetics of this transformation has been reported. The XRD measurements are compared with forward diffraction simulations to evaluate different proposed phase transformation mechanisms and are shown to be consistent with a shearing mechanism that results in the RS [001] direction being aligned with the original WZ 𝑐-axis and the RS [110] direction being aligned with the original WZ 𝑎-axis. In order to reproduce all observed RS diffraction spots the forward diffraction simulations required three different RS crystalline domains, each with a [110] direction aligned with one of the three original WZ 𝑎-axes, and a significant mosaicity, particularly about the RS [001] direction (the direction of shock propagation). In conclusion, this large mosaicity is likely due to variations in the possible shearing mechanisms that connect the WZ and RS structures, which can create crystallites with up to ∼ ±10° of relative rotations about the RS [001] direction, as well as further rotation of these crystallites as they grow, merge, and coalesce.

Crystal structure↗

BTE-Sim: Fast Simulation Environment For Public Transportation

The public commute is essential to all urban centers and is an efficient and environment-friendly way to travel. Transit systems must become more accessible and user-friendly. Since public transit is majorly designed statically, with very few improvements coming over time, it can get stagnated, unable to update itself with changing population trends. To better understand transportation demands and make them more usable, efficient, and demographic-focused, we propose a fast, multi-layered transit simulation that primarily focuses on public transit simulation (BTE-Sim). BTE-Sim is designed based on the population demand, existing traffic conditions, and the road networks that exist in a region. The system is versatile, with the ability to run different configurations of the existing transit routes, or inculcate any new changes that may seem necessary, or even in extreme cases, new transit network design as well. In all situations, it can compare multiple transit networks and provide evaluation metrics for them. It provides detailed data on each transit vehicle, the trips it performs, its on-time performance and other necessary factors. Its highlighting feature is the considerably low computation time it requires to perform all these tasks and provide consistently reliable results.

Sen, Rishav↗

Distributed Outage Detection in Power Distribution Networks

Real time topology knowledge is essential for situational awareness of power distribution networks. Line outages change the topology of a distribution network. Hence, outage detection is an important task. Most of the existing outage detection algorithms are centralized, in which sensors communicate their data to a control center which performs outage detection using the received data. However, with the increasing size of the distribution network and with different areas of the network being monitored by different operators, communication is a bottleneck and scalability is a major concern. To address these issues, we propose a novel outage detection algorithm using a divide and conquer approach. First, we divide a distribution network into sub-networks, such that outage detection can be run in parallel in each sub-network independently ensuring scalability to large networks. Further, to reduce the latency, bandwidth and attenuation challenges associated with communications in a large network, we divide each sub-network into multiple control areas which communicate only with their neighbors. We employ a distributed iterative load estimation across the control areas of each sub-network and then use the load estimate for local outage detection in each control area. Here, the performance of our algorithm is evaluated for multiple feeder models and compared against traditional centralized outage detection algorithms.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Understanding water and energy fluxes in the Amazonia: Lessons from an observation‐model intercomparison

Abstract Tropical forests are an important part of global water and energy cycles, but the mechanisms that drive seasonality of their land‐atmosphere exchanges have proven challenging to capture in models. Here, we (1) report the seasonality of fluxes of latent heat (LE), sensible heat ( H ), and outgoing short and longwave radiation at four diverse tropical forest sites across Amazonia—along the equator from the Caxiuanã and Tapajós National Forests in the eastern Amazon to a forest near Manaus, and from the equatorial zone to the southern forest in Reserva Jaru; (2) investigate how vegetation and climate influence these fluxes; and (3) evaluate land surface model performance by comparing simulations to observations. We found that previously identified failure of models to capture observed dry‐season increases in evapotranspiration (ET) was associated with model overestimations of (1) magnitude and seasonality of Bowen ratios (relative to aseasonal observations in which sensible was only 20%–30% of the latent heat flux) indicating model exaggerated water limitation, (2) canopy emissivity and reflectance (albedo was only 10%–15% of incoming solar radiation, compared to 0.15%–0.22% simulated), and (3) vegetation temperatures (due to underestimation of dry‐season ET and associated cooling). These partially compensating model‐observation discrepancies (e.g., higher temperatures expected from excess Bowen ratios were partially ameliorated by brighter leaves and more interception/evaporation) significantly biased seasonal model estimates of net radiation ( R n ), the key driver of water and energy fluxes (LE ~ 0.6 R n and H ~ 0.15 R n ), though these biases varied among sites and models. A better representation of energy‐related parameters associated with dynamic phenology (e.g., leaf optical properties, canopy interception, and skin temperature) could improve simulations and benchmarking of current vegetation–atmosphere exchange and reduce uncertainty of regional and global biogeochemical models.

Restrepo‐Coupe, Natalia↗

Influence of Correlations on the Thermal Performance Modeling of Parabolic Trough Collectors

The influence of correlations on the thermal performance modeling of parabolic trough collectors was analyzed in this work. A versatile model for a parabolic trough collector was developed that allows one- and two-dimensional analysis and enables the use of correlations to calculate thermophysical properties and convection heat transfer coefficients. The model also allows the use of constant values for properties and/or coefficients obtained from the evaluation correlations at a specific temperature. The effect of each correlation was evaluated independently, and the results were compared with a reference case that considered a two-dimensional approach and used all the correlations. For the analyzed cases, the correlation for the absorber emittance has the strongest impact on the collector efficiency, leading to a lower error when used. Based on the results, a one-dimensional model approach considering a correlation for the absorber emittance leads to efficiency errors below 3% for collector lengths of up to 243.6 m. Compared with the reference case, a one-dimensional approach using all correlations for a collector with a length of 500 m, and operating with an inlet temperature of 773 K, can result in errors around 9%. However, using constant values for properties and heat transfer coefficients could lead to errors of up to 50%. Multiple thermal models for parabolic trough collectors proposed in the literature rely on a one-dimensional approach, estimated values for the heat transfer coefficients, and constant thermophysical properties. The errors associated with those approaches are analyzed and quantified in this work as a function of the collector length and operation temperature.

absorber emittance↗

Effect of gap size on composition and structure of regeneration 19 years after harvest in a southeastern bottomland forest, USA

Following timber harvests in bottomland mixed-oak (Quercus L.) stands, desirable oak advance regeneration can be overgrown by shade-intolerant pioneer species. We investigated the effects of group selection opening size on composition of tree regeneration 19 years postharvest in an oak-dominated bottomland forest and compared results with earlier findings to evaluate development trends. In response to six opening sizes (7–40 m radii), we evaluated regeneration density and dominance of four tree species groups: conifers, hard mast, shade-intolerant hardwoods, and shade-tolerant hardwoods. Our objectives were to determine the optimum gap size for regenerating oaks and test the delayed oak dominance hypothesis, in which oaks slowly gain dominance as pioneer species undergo self-thinning. Opening size influenced conifer regeneration but minimally affected hardwoods. Hard mast species density was less than that of either intolerant or tolerant species regardless of opening size. Future stem density trends suggest increasing intolerant species and constant mast and tolerant species. Modeled future height trends suggest increasing mast species dominance over intolerant pioneers after 30 years. Furthermore, our results suggest that gap-based silviculture can be used to regenerate bottomland hardwood stands with desirable species including oaks; larger gaps favor conifers but there was no optimum size to enhance oak regeneration.

60 APPLIED LIFE SCIENCES↗

pnnl/neural_ODE_ICLR2020

We show how to model discrete ordinary differential equations (ODE) with algebraic nonlinearities as deep neural networks with varying degrees of prior knowledge. We derive the stability guarantees of the network layers based on the implicit constraints imposed on the weight's eigenvalues. Moreover, we show how to use barrier methods to generically handle additional inequality constraints. We demonstrate the prediction accuracy of learned neural ODEs evaluated on open-loop simulations compared to ground truth dynamics with bi-linear terms.

Tuor, Aaron↗

HPC ODA Commons [SWR-26-003]

HPC ODA Commons is a community-driven platform for standardizing HPC operational data analytics. HPC sites generate enormous volumes of operational data - scheduler logs, accounting records, monitoring streams - but turning that data into actionable insight is needlessly hard. Each site builds bespoke parsers, schemas, and evaluation pipelines. Results can't be compared across institutions. Promising analytics ideas stay siloed because there's no shared language for describing the data, the experiments, or the outcomes. HPC ODA Commons fixes this by establishing community-governed contracts - versioned schemas, canonical artifacts, and benchmark recipes - that make ODA workflows discoverable, reproducible, and comparable. It pairs these standards with a practical, CLI-first toolkit that lets operators and researchers go from raw logs to standardized results without sending data off-cluster.

Menear, Kevin [National Laboratory of the Rockies ↗

ENDF/B-VIII.1: Proton Reaction Sublibrary

The protons sublibrary aims to describe nuclear reactions between incident proton particles and different nuclei. For ENDF/B-VIII.1, compared to ENDF/B-VIII.0, there were only evaluation updates for 4 He.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

ENDF/B-VIII.1: Triton Reaction Sublibrary

The tritons sublibrary aims to describe nuclear reactions between incident triton particles and different nuclei. For ENDF/B-VIII.1, compared to ENDF/B-VIII.0, there were only evaluation updates for 4 He.

73 NUCLEAR PHYSICS AND RADIATION PHYSICS↗

A Bimodal Diagnostic Cloud Fraction Parameterization. Part I: Motivating Analysis and Scheme Description

Cloud fraction parameterizations are beneficial to regional, convection-permitting numerical weather prediction. For its operational regional midlatitude forecasts, the Met Office uses a diagnostic cloud fraction scheme that relies on a unimodal, symmetric subgrid saturation-departure distribution. This scheme has been shown before to underestimate cloud cover and hence an empirically based bias correction is used operationally to improve performance. This first of a series of two papers proposes a new diagnostic cloud scheme as a more physically based alternative to the operational bias correction. The new cloud scheme identifies entrainment zones associated with strong temperature inversions. Additionally, for model grid boxes located in this entrainment zone, collocated moist and dry Gaussian modes are used to represent the subgrid conditions. The mean and width of the Gaussian modes, inferred from the turbulent characteristics, are then used to diagnose cloud water content and cloud fraction. It is shown that the new scheme diagnoses enhanced cloud cover for a given gridbox mean humidity, similar to the current operational approach. It does so, however, in a physically meaningful way. Using observed aircraft data and ground-based retrievals over the southern Great Plains in the United States, it is shown that the new scheme improves the relation between cloud fraction, relative humidity, and liquid water content. An emergent property of the scheme is its ability to infer skewed and bimodal distributions from the large-scale state that qualitatively compare well against observations. A detailed evaluation and resolution sensitivity study will follow in Part II.

54 ENVIRONMENTAL SCIENCES↗

What to measure and report in studies of discomfort from glare for pedestrian applications

We report in outdoor environments after dark, pedestrians may experience discomfort from glare caused by lighting. Several models to predict discomfort from glare have been proposed or extended for pedestrian applications; these models use different luminous and geometrical quantities to predict discomfort. Consistent measurements and reporting in studies of discomfort from glare are important for identifying best performing models; however, previous studies proposing a new model tended to only report the performance of the new model and its quantities. This practice makes it difficult to evaluate how a new model performs compared to other existing models. To promote more consistent and complete reporting, this research note proposes measuring and reporting all relevant quantities that are used in existing models. This can make it easier for researchers to use a study dataset to compare the performance of several models or to combine datasets from several studies to address between-study variance.

42 ENGINEERING↗

Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

Abstract Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae , O. polymorpha , and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha , as well as Saccharomyces cerevisiae , and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

09 BIOMASS FUELS↗

VECTOR Phase 1 Dataset: CAV Trajectory and Energy Consumption Records

This dataset contains benchmark experimental data from Phase 1 of the VECTOR project, focusing on the energy impact of CAV hardware components. The dataset includes vehicle trajectory data (speed and position) and corresponding energy consumption records collected from a CAV platform equipped with lidar, cameras, onboard computation units, and communication modules. The primary objective is to quantify the baseline energy consumption attributable to sensing and computing systems, independent of any advanced cooperative control strategies. During experiments, the leading vehicle followed a predetermined velocity profile, and the following CAV mirrored this trajectory using a basic car-following control to ensure consistent driving behavior. This setup enables a reliable benchmark for assessing the energy cost introduced by onboard CDA hardware (e.g., lidar and GPU-based processing). The dataset is essential for evaluating energy baselines and supports future comparative studies involving additional cooperative strategies. ![system img](system.png) ![vector img](vector.png)

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

EXPERIMENTAL INVESTIGATION OF IMPACT AND ABRASION EROSIONS AT LOW VELOCITIES AND COMPARISON TO CFD SIMULATIONS

Wear and erosion damage of materials due to interaction of the surface with solid particles is a major issue in various industries. This phenomenon is common in mining, and oil and gas production, causing damages worth millions of dollars annually. Erosion is becoming a critical issue in renewable technologies as well such as concentrated solar thermal power (CSP) systems. In some CSP systems, solid particles can be used to absorb solar energy and enhance the heat storage. However, these particles may cause significant amount of wear to system components while falling down the power tower at high temperatures. This damage can be costly, and therefore, investigating solid particle erosion in CSP systems is of utmost importance. In collaboration with Sandia National Laboratories, the erosion durability of particle-based Generation 3 (GEN3) CSP system are being investigated both experimentally and numerically. In order to develop models to predict the life time of such systems, erosion under low temperature and low particle impact velocity conditions are investigated. Impact erosion and abrasion erosion experiments are conducted for low velocity and low temperature conditions for various candidate particles and containment materials. The impact erosion testing is conducted for three different containment material including Stainless Steel 316, special refractory material, and Inconel 740H with CARBOBEAD HSP 40/70 ceramic particles and UNIWEST 430 silica quartz erodent particles. All this data will enable us to better model the erosion behavior of these materials and provides a data base to investigate erosion at high temperatures experienced in CSP systems. At low temperatures and low velocities the softer refractory material exhibited greater erosion by an order-of-magnitude when compared to the harder Inconel 740H and Stainless Steel 316 materials. The overall erosion ratio for all the candidate materials was of the order of 10 -7 kg/kg. We also investigated wear in containment materials from sliding of particles over the surfaces at very low angles, also referred to as abrasion. For the velocities expected in a typical 1MW CSP plant, visible surface wear was noted, but the overall mass loss per unit surface area was found to be dependent on the hardness of the containment material tested. In this manuscript, we also present Computational Fluid Dynamics (CFD) simulations and erosion prediction of low velocity impact erosion. These results are compared with the experimental data to evaluate the ability of available erosion models for predicting erosion under these conditions.

14 SOLAR ENERGY↗

Concentric Ring Gas Atomization Die Design for Optimized Particle Production

In partnership with Linde plc (formerly Praxair, Inc.), Ames Laboratory will further develop and commercialize its concentric-ring high pressure gas atomization (CR-HPGA) gas-die technology with a goal to improve the precision of metal powder production in a desired size range and quality. The successful commercialization of this technology could reduce production cost of metal powders and improve reliability of this industrial process, increasing specialty alloy powder availability for new applications and adoption into additive manufacturing and multiple materials sectors. The project scope is to [1] utilize AMES compressible gas flow and melt break-up models to explore a wide swath of parameter space available for the CR-HPGA technology, identifying the most promising gas-die designs for fabrication, [2] verify and optimize the new gas-die design, fabricate it and select preferred operating parameters by gas-only flow imaging and aspiration pressure measurements, down-selecting gas compositions and atomization parameters for full scale testing, [3] perform pilot-scale atomization trials with Al and/or Cu alloys of a new CR-HPGA gas-die at selected parameters, [4] evaluate gas-die performance by comparing resulting powder size distribution and powder quality attributes with equivalent powders made by conventional close-coupled HPGA technology, and [5] assess (through Praxair partnership) reduction in operational costs of new CR-HPGA gas-die through the use of an inert gas recovery/recycling system. In partnership with Praxair, Inc., the team, led by Ames Laboratory senior metallurgist Iver Anderson, intend to demonstrate the performance of an optimized CR-HPGA gas-die design in a pilot scale atomizer and better understand the design and operational controls to increase overall benefits for precision metal powder production.

42 ENGINEERING↗

Benchmarking the AirSquared All-Metal Scroll Pump Against the Normetex 15 - Preliminary report

Tritium operations require specialized equipment for material compatibility, safety, and waste considerations. Non-metallic pump materials (polymer, oil, or grease) on a tritium-wetted surface can absorb tritium and/or undergo isotope exchange between tritium and more common protium, creating tritiated compounds that can result in material property modification via beta-radiolysis, inventory loss, reduced performance and special waste considerations, all of which increase the operating cost for a facility. Vacuum pumps move gas via a pressure differential between volumes of an operation. Several types of all-metal vacuum pumps exist for this application including metal bellows, diaphragm and scroll pumps. A metal bellows pump incorporates only metal components at the wetted surfaces. The common metal bellows pump employed in tritium facilities, the MB601 (Senior Metal Bellows, Sharon, PA, USA) creates a gas-motive force with a vacuum pressure between 30-40 torr. These pumps can efficiently move gas from one process step to another, as long as sub-30 torr pressures are not required. However, for higher vacuum demand, an all-metal scroll pump can be used with the bellows pump serving as a “backing” pump. This configuration typically provides an achievable vacuum of less than 20 mTorr. All-metal scroll pumps are a technology uniquely applicable to tritium gas handling. The industry standard all-metal scroll pump for glovebox-scale tritium operations is the Normetex 15 m 3 /hr, typically referred to as the Normetex 15 (Normetex Pompes, Pont Audemer, France). However, the final Normetex pumps were manufactured in 2012, rendering them no longer available. Eumeca SaRL (formerly Normetex) has developed and is marketing a scroll pump based on the design of the Normetex 15. A US company, AirSquared (Broomfield, Colorado), has developed and is marketing a potential alternative to the Normetex 15. The Gas Transfer Systems group (Q-7) of the Los Alamos National Laboratory (LANL) procured serial number 0001 of the AirSquared All Metal Vacuum Scroll pump model number V16H034A-C01 in 2019. The LANL Hydrogen Processing Laboratory (HPL) has been evaluating the performance of the pump compared to a Normetex pump as a potential replacement in tritium and fusion facilities requiring this type of pump.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗