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At least 703 records · Page 39

Mechanisms of Chromium and Uranium Toxicity in Pseudomonas stutzeri RCH2 Grown under Anaerobic Nitrate-Reducing Conditions

Chromium and uranium are highly toxic metals that contaminate many natural environments. We investigated their mechanisms of toxicity under anaerobic conditions using nitrate-reducing Pseudomonas stutzeri RCH2, which was originally isolated from a chromium-contaminated aquifer. A random barcode transposon site sequencing library of RCH2 was grown in the presence of the chromate oxyanion (Cr[VI]O$2-\atop{4}$) or uranyl oxycation (U[VI]O$2+\atop{2}$). Strains lacking genes required for a functional nitrate reductase had decreased fitness as both metals interacted with heme-containing enzymes required for the later steps in the denitrification pathway after nitrate is reduced to nitrite. Cr[VI]-resistance also required genes in the homologous recombination and nucleotide excision DNA repair pathways, showing that DNA is a target of Cr[VI] even under anaerobic conditions. The reduced thiol pool was also identified as a target of Cr[VI] toxicity and psest_2088, a gene of previously unknown function, was shown to have a role in the reduction of sulfite to sulfide. U[VI] resistance mechanisms involved exopolysaccharide synthesis and the universal stress protein UspA. Finally, as the first genome-wide fitness analysis of Cr[VI] and U[VI] toxicity under anaerobic conditions, this study provides new insight into the impact of Cr[VI] and U[VI] on an environmental isolate from a chromium contaminated site, as well as into the role of a ubiquitous protein, Psest_2088.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Environmental Tuning of Homologs of the Orange Carotenoid Protein-Encoding Gene in the Cyanobacterium Fremyella diplosiphon

The orange carotenoid protein (OCP) family of proteins are light-activated proteins that function in dissipating excess energy absorbed by accessory light-harvesting complexes, i.e., phycobilisomes (PBSs), in cyanobacteria. Some cyanobacteria contain multiple homologs of the OCP-encoding gene ( ocp ). Fremyella diplosiphon , a cyanobacterium studied for light-dependent regulation of PBSs during complementary chromatic acclimation (CCA), contains several OCP homologs – two full-length OCPs, three Helical Carotenoid Proteins (HCPs) with homology to the N-terminus of OCP, and one C-terminal domain-like carotenoid protein (CCP) with homology to the C-terminus of OCP. We examined whether these homologs are distinctly regulated in response to different environmental factors, which could indicate distinct functions. We observed distinct patterns of expression for some OCP, HCP, and CCP encoding genes, and have evidence that light-dependent aspects of ocp homolog expression are regulated by photoreceptor RcaE which controls CCA. RcaE-dependent transcriptional regulator RcaC is also involved in the photoregulation of some hcp genes. Apart from light, additional environmental factors associated with cellular redox regulation impact the mRNA levels of ocp homologs, including salt, cold, and disruption of electron transport. Analyses of conserved sequences in the promoters of ocp homologs were conducted to gain additional insight into regulation of these genes. Several conserved regulatory elements were found across multiple ocp homolog promoters that potentially control differential transcriptional regulation in response to a range of environmental cues. The impact of distinct environmental cues on differential accumulation of ocp homolog transcripts indicates potential functional diversification of this gene family in cyanobacteria. These genes likely enable dynamic cellular protection in response to diverse environmental stress conditions in F . diplosiphon .

59 BASIC BIOLOGICAL SCIENCES↗

Fluorogenic Biosensing with Tunable Polydiacetylene Vesicles

Polydiacetylenes (PDAs) are conjugated polymers that are well known for their colorimetric transition from blue to red with the application of energetic stimulus. Sensing platforms based on polymerized diacetylene surfactant vesicles and other structures have been widely demonstrated for various colorimetric biosensing applications. Although less studied and utilized, the transition also results in a change from a non-fluorescent to a highly fluorescent state, making polydiacetylenes useful for both colorimetric and fluorogenic sensing applications. Here, we focus on the characterization and optimization of polydiacetylene vesicles to tune their sensitivity for fluorogenic sensing applications. Particularly, we look at how the structure of the diacetylene (DA) hydrocarbon tail and headgroup affect the self-assembled vesicle size and stability, polymerization kinetics, and the fluorogenic, blue to red phase transition. Longer DA acyl tails generally resulted in smaller and more stable vesicles. The polymerization kinetics and the blue to red transition were a function of both the DA acyl tail length and structure of the headgroup. Decreasing the acyl tail length generally led to vesicles that were more sensitive to energetic stimuli. Headgroup modifications had different effects depending on the structure of the headgroup. Ethanolamine headgroups resulted in vesicles with potentially increased stimuli responsivity. The lower energy stimulus to induce the chromatic transition was attributed to an increase in headgroup hydrogen bonding and polymer backbone strain. Boronic-acid headgroup functionalization led to vesicles that were generally unstable, only weakly polymerized, and unable to fully transform to the red phase due to strong polar, aromatic headgroup interactions. This work presents the design of PDA vesicles in the context of biosensing platforms and includes a discussion of the past, present, and future of PDA biosensing.

Chemistry↗

Atmospheric Pressure Plasma Treatment of Magnesium Alloy for Enhanced Coating Adhesion and Corrosion Resistance

Atmospheric pressure plasma (AP) treatment, using an open-air jet of ionized CO 2 , N 2 , or air, was applied to AZ91D Mg alloy surfaces to investigate its effects on primer coating adhesion and corrosion resistance. The CO 2 and air AP treatments formed an O- and C-rich surface layer (Mg-O-C) consisting of agglomerated nanoparticles and pits with a depth of a few microns and increasing the surface roughness by 6–8 times compared with the reference 600 grit-finished surface. Then, three commercial primers, zinc phosphate (ZnP), chromate-containing epoxy, and MIL23377, were applied on the treated surfaces to evaluate the corrosion resistance associated with the coating adhesion. Microscopic analysis demonstrated stronger interlocking between the primer layer and the nano-/microrough Mg-O-C surface compared to the untreated (600 grit-finished) surfaces, indicating improved coating adhesion and corrosion resistance. Crosscut tests of the MIL23377 primer on the CO 2 and air AP-treated surfaces showed the highest level of adhesion, ASTM class 5B. Salt spray corrosion tests showed that after 8 days of exposure, the primer coatings on air AP-treated surfaces had corrosion areas that were more than four times smaller than that of the 600 grit-finished surface. The N 2 AP treatment showed similar adhesion enhancement. The preliminary operation expenses for AP treatment using CO 2 , N 2 , and air were estimated at USD 30.62, USD 35.45, and USD 29.75 (from an air cylinder)/USD 0.66 (from an air compressor) per m 2 , respectively.

36 MATERIALS SCIENCE↗

Homologs of Phycobilisome Abundance Regulator PsoR Are Widespread across Cyanobacteria

During chromatic acclimation (CA), cyanobacteria undergo shifts in their physiology and metabolism in response to changes in their light environment. Various forms of CA, which involves the tuning of light-harvesting accessory complexes known as phycobilisomes (PBS) in response to distinct wavelengths of light, have been recognized. Recently, a negative regulator of PBS abundance, PsoR, about which little was known, was identified. We used sequence analyses and bioinformatics to predict the role of PsoR in cyanobacteria and PBS regulation and to examine its presence in a diverse range of cyanobacteria. PsoR has sequence similarities to the β-CASP family of proteins involved in DNA and RNA processing. PsoR is a putative nuclease widespread across Cyanobacteria, of which over 700 homologs have been observed. Promoter analysis suggested that psoR is co-transcribed with upstream gene tcpA. Multiple transcription factors involved in global gene regulation and stress responses were predicted to bind to the psoR-tcpA promoter. The predicted protein–protein interactions with PsoR homologs included proteins involved in DNA and RNA metabolism, as well as a phycocyanin-associated protein predicted to interact with PsoR from Fremyella diplosiphon (FdPsoR). The widespread presence of PsoR homologs in Cyanobacteria and their ties to DNA- and RNA-metabolizing proteins indicated a potentially unique role for PsoR in CA and PBS abundance regulation.

Layer, Alicia↗

Comparison of Phenology Estimated from Reflectance-Based Indices and Solar-Induced Chlorophyll Fluorescence (SIF) Observations in a Temperate Forest Using GPP-Based Phenology as the Standard

We assessed the performance of reflectance-based vegetation indices and solar-induced chlorophyll fluorescence (SIF) datasets with various spatial and temporal resolutions in monitoring the Gross Primary Production (GPP)-based phenology in a temperate deciduous forest. The reflectance-based indices include the green chromatic coordinate (GCC), field measured and satellite remotely sensed Normalized Difference Vegetation Index (NDVI); and the SIF datasets include ground-based measurement and satellite-based products. We found that, if negative impacts due to coarse spatial and temporal resolutions are effectively reduced, all these data can serve as good indicators of phenological metrics for spring. However, the autumn phenological metrics derived from all reflectance-based datasets are later than the those derived from ground-based GPP estimates (flux sites). This is because the reflectance-based observations estimate phenology by tracking physiological properties including leaf area index (LAI) and leaf chlorophyll content (Chl), which does not reflect instantaneous changes in phenophase transitions, and thus the estimated fall phenological events may be later than GPP-based phenology. In contrast, we found that SIF has a good potential to track seasonal transition of photosynthetic activities in both spring and fall seasons. The advantage of SIF in estimating the GPP-based phenology lies in its inherent link to photosynthesis activities such that SIF can respond quickly to all factors regulating phenological events. Despite uncertainties in phenological metrics estimated from current spaceborne SIF observations due to their coarse spatial and temporal resolutions, dates in middle spring and autumn—the two most important metrics—can still be reasonably estimated from satellite SIF. Our study reveals that SIF provides a better way to monitor GPP-based phenological metrics.

59 BASIC BIOLOGICAL SCIENCES↗

Using Near-Infrared-Enabled Digital Repeat Photography to Track Structural and Physiological Phenology in Mediterranean Tree–Grass Ecosystems

Tree–grass ecosystems are widely distributed. However, their phenology has not yet been fully characterized. The technique of repeated digital photographs for plant phenology monitoring (hereafter referred as PhenoCam) provide opportunities for long-term monitoring of plant phenology, and extracting phenological transition dates (PTDs, e.g., start of the growing season). Here, we aim to evaluate the utility of near-infrared-enabled PhenoCam for monitoring the phenology of structure (i.e., greenness) and physiology (i.e., gross primary productivity—GPP) at four tree–grass Mediterranean sites. We computed four vegetation indexes (VIs) from PhenoCams: (1) green chromatic coordinates (GCC), (2) normalized difference vegetation index (CamNDVI), (3) near-infrared reflectance of vegetation index (CamNIRv), and (4) ratio vegetation index (CamRVI). GPP is derived from eddy covariance flux tower measurement. Then, we extracted PTDs and their uncertainty from different VIs and GPP. The consistency between structural (VIs) and physiological (GPP) phenology was then evaluated. CamNIRv is best at representing the PTDs of GPP during the Green-up period, while CamNDVI is best during the Dry-down period. Moreover, CamNIRv outperforms the other VIs in tracking growing season length of GPP. In summary, the results show it is promising to track structural and physiology phenology of seasonally dry Mediterranean ecosystem using near-infrared-enabled PhenoCam. We suggest using multiple VIs to better represent the variation of GPP.

47 OTHER INSTRUMENTATION↗

Phenocams Bridge the Gap between Field and Satellite Observations in an Arid Grassland Ecosystem

Near surface (i.e., camera) and satellite remote sensing metrics have become widely used indicators of plant growing seasons. While robust linkages have been established between field metrics and ecosystem exchange in many land cover types, assessment of how well remotely-derived season start and end dates depict field conditions in arid ecosystems remain unknown. We evaluated the correspondence between field measures of start (SOS; leaves unfolded and canopy greenness >0) and end of season (EOS) and canopy greenness for two widespread species in southwestern U.S. ecosystems with those metrics estimated from near-surface cameras and MODIS NDVI for five years (2012–2016). Using Timesat software to estimate SOS and EOS from the phenocam green chromatic coordinate (GCC) greenness index resulted in good agreement with ground observations for honey mesquite but not black grama. Despite differences in the detectability of SOS and EOS for the two species, GCC was significantly correlated with field estimates of canopy greenness for both species throughout the growing season. MODIS NDVI for this arid grassland site was driven by the black grama signal although a mesquite signal was discernable in average rainfall years. Our findings suggest phenocams could help meet myriad needs in natural resource management.

ecosystem productivity↗

Exploiting the Autofluorescent Properties of Photosynthetic Pigments for Analysis of Pigmentation and Morphology in Live Fremyella diplosiphon Cells

Fremyella diplosiphon is a freshwater, filamentous cyanobacterium that exhibits light-dependent regulation of photosynthetic pigment accumulation and cellular and filament morphologies in a well-known process known as complementary chromatic adaptation (CCA). One of the techniques used to investigate the molecular bases of distinct aspects of CCA is confocal laser scanning microscopy (CLSM). CLSM capitalizes on the autofluorescent properties of cyanobacterial phycobiliproteins and chlorophyll a. We employed CLSM to perform spectral scanning analyses of F. diplosiphon strains grown under distinct light conditions. We report optimized utilization of CLSM to elucidate the molecular basis of the photoregulation of pigment accumulation and morphological responses in F. diplosiphon.

fluorescence imaging↗

Multi-wavelength observations of GRB 111228A and implications for the fireball and its environment

Observations of very early multi-wavelength afterglows are critical to reveal the properties of the radiating fireball and its environment as well as the central engine of gamma-ray bursts (GRBs). We report our optical observations of GRB 111228A from 95 s to about 50 hr after the burst trigger and investigate its properties of the prompt gamma-rays and the ambient medium using our data and the data from the Swift and Fermi missions. Our joint optical and X-ray spectral fits to the afterglow data show that the ambient medium features a low dust-to-gas ratio. Incorporating the energy injection effect, our best fit to the afterglow light curves with the standard afterglow model via the Markov Chain Monte Carlo technique shows that ϵ{sub e}=(6.9±0.3)×10{sup −2}, ϵ{sub B}=(7.73±0.62)×10{sup −6},E{sub K}=(6.32±0.86)×10{sup 53} erg, n=0.100±0.014 cm{sup −3}. The low medium density likely implies that the afterglow jet may be in a halo or in a hot ISM. A chromatic shallow decay segment observed in the optical and X-ray bands is well explained with the long-lasting energy injection from the central engine, which would be a magnetar with a period of about 1.92 ms inferred from the data. The E{sub p} of its time-integrated prompt gamma-ray spectrum is ∼26 KeV. Using the initial Lorentz factor (Γ{sub 0}=476{sub −237}{sup +225}) derived from our afterglow model fit, it is found that GRB 111228A satisfies the L{sub iso}−E{sub p,z}−Γ{sub 0} relation and bridges the typical GRBs and low luminosity GRBs in this relation.

79 ASTRONOMY AND ASTROPHYSICS↗

Structure of the accretion disk in the lensed quasar Q2237+0305 from multi-epoch and multi-wavelength narrowband photometry

We present estimates for the size and the logarithmic slope of the disk temperature profile of the lensed quasar Q2237+0305, independent of the component velocities. These estimates are based on six epochs of multi-wavelength narrowband images from the Nordic Optical Telescope. For each pair of lensed images and each photometric band, we determine the microlensing amplitude and chromaticity using pre-existing mid-IR photometry to define the baseline for no microlensing magnification. A statistical comparison of the combined microlensing data (6 epochs × 5 narrow bands × 6 image pairs) with simulations based on microlensing magnification maps gives Bayesian estimates for the half-light radius of R{sub 1/2}=8.5{sub −4.0}{sup +7.5}√(〈M〉/0.3 M{sub ⊙}) lt-day, and p = 0.95 ± 0.33 for the exponent of the logarithmic temperature profile T∝R{sup −1/p}. This size estimate is in good agreement with most recent studies. Other works based on the study of single microlensing events predict smaller sizes, but could be statistically biased by focusing on high-magnification events.

79 ASTRONOMY AND ASTROPHYSICS↗

Observing Exoplanets with High Dispersion Coronagraphy. I. The Scientific Potential of Current and Next-generation Large Ground and Space Telescopes

Direct imaging of exoplanets presents a formidable technical challenge owing to the small angular separation and high contrast between exoplanets and their host stars. High Dispersion Coronagraphy (HDC) is a pathway to achieve unprecedented sensitivity to Earth-like planets in the habitable zone. Here, we present a framework to simulate HDC observations and data analyses. The goal of these simulations is to perform a detailed analysis of the trade-off between raw star light suppression and spectral resolution for various instrument configurations, target types, and science cases. We predict the performance of an HDC instrument at Keck observatory for characterizing directly imaged gas-giant planets in near-infrared bands. We also simulate HDC observations of an Earth-like planet using next-generation ground-based (TMT) and spaced-base telescopes (HabEx and LUVOIR). We conclude that ground-based ELTs are more suitable for HDC observations of an Earth-like planet than future space-based missions owing to the considerable difference in collecting area. For ground-based telescopes, HDC observations can detect an Earth-like planet in the habitable zone around an M-dwarf star at 10{sup −4} star light suppression level. Compared to the 10{sup −7} planet/star contrast, HDC relaxes the star light suppression requirement by a factor of 10{sup 3}. For space-based telescopes, detector noise will be a major limitation at spectral resolutions higher than 10{sup 4}. Considering detector noise and speckle chromatic noise, R = 400 (1600) is the optimal spectral resolutions for HabEx (LUVOIR). The corresponding star light suppression requirement to detect a planet with planet/star contrast = 6.1×10{sup −11} is relaxed by a factor of 10 (100) for HabEx (LUVOIR).

79 ASTRONOMY AND ASTROPHYSICS↗

Forward Global Photometric Calibration of the Dark Energy Survey

Many scientific goals for the Dark Energy Survey (DES) require the calibration of optical/NIR broadband b = grizY photometry that is stable in time and uniform over the celestial sky to one percent or better. It is also necessary to limit to similar accuracy systematic uncertainty in the calibrated broadband magnitudes due to uncertainty in the spectrum of the source. Here we present a "Forward Global Calibration Method (FGCM)" for photometric calibration of the DES, and we present results of its application to the first three years of the survey (Y3A1). The FGCM combines data taken with auxiliary instrumentation at the observatory with data from the broadband survey imaging itself and models of the instrument and atmosphere to estimate the spatial and time dependences of the passbands of individual DES survey exposures. "Standard" passbands that are typical of the passbands encountered during the survey are chosen. The passband of any individual observation is combined with an estimate of the source spectral shape to yield a magnitude in the standard system. This "chromatic correction" to the standard system is necessary to achieve subpercent calibrations and in particular, to resolve ambiguity between the broadband brightness of a source and the shape of its SED. The FGCM achieves a reproducible and stable photometric calibration of standard magnitudes of stellar sources over the multiyear Y3A1 data sample with residual random calibration errors of per exposure. The accuracy of the calibration is uniform across the DES footprint to within . The systematic uncertainties of magnitudes in the standard system due to the spectra of sources are less than for main-sequence stars with .

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Exoplanets in the Antarctic Sky. IV. Dual-band Photometry of Variables Found by the CSTAR-II Commissioning Survey at the North Sky

From the experiences learned in three decades of exoplanet search, wide-field transit surveys have proven to be one of the most effective ways to detect exoplanets. Wide field of view, however, suffers from high false-positive rates caused by blended eclipsing binaries. The chromaticity in eclipse depth is an effective feature to distinguish low-depth eclipsing binaries from transiting exoplanets, making multiple-band photometry follow-up advantageous before a target is passed onto more expensive spectroscopic follow-up. Moreover, a multiple-band photometric survey is itself a powerful method to find and vet planetary candidates and narrow down the candidate list of high-priority targets. In this work, we report the first results of a dual-band (Sloan-g and -i) wide-field photometry survey—the Chinese Small Telescope ARray II (CSTAR-II), an updated version of the original CSTAR. As a key component of the Chinese Exoplanet Searching Program from Antarctica, CSTAR-II has been tested thoroughly at a remote arctic site near Mohe during the winter of 2014. In total, 13,531 light curves with the best overall photometric precision of ∼3 mmag were extracted from 7721 stars in the Sloan-g and -i bands. Using a robust method, we have detected 63 variables, of which 48 are newly discovered. The dual-band photometric results as well as the stellar properties of the detected sources are provided in this work.

79 ASTRONOMY AND ASTROPHYSICS↗

Performance Verification of the EXtreme PREcision Spectrograph

The EXtreme PREcision Spectrograph (EXPRES) is a new Doppler spectrograph designed to reach a radial-velocity measurement precision sufficient to detect Earth-like exoplanets orbiting nearby, bright stars. We report on extensive laboratory testing and on-sky observations to quantitatively assess the instrumental radial-velocity measurement precision of EXPRES, with a focused discussion of individual terms in the instrument error budget. We find that EXPRES can reach a single-measurement instrument calibration precision better than 10 cm s{sup −1}, not including photon noise from stellar observations. We also report on the performance of the various environmental, mechanical, and optical subsystems of EXPRES, assessing any contributions to radial-velocity error. For atmospheric and telescope related effects, this includes the fast tip-tilt guiding system, atmospheric dispersion compensation, and the chromatic exposure meter. For instrument calibration, this includes the laser fRequency comb (LFC), flat-field light source, CCD detector, and effects in the optical fibers. Modal noise is mitigated to a negligible level via a chaotic fiber agitator, which is especially important for wavelength calibration with the LFC. Regarding detector effects, we empirically assess the impact on the radial-velocity precision due to pixel-position nonuniformities and charge transfer inefficiency (CTI). EXPRES has begun its science survey to discover exoplanets orbiting G-dwarf and K-dwarf stars, in addition to transit spectroscopy and measurements of the Rossiter–McLaughlin effect.

79 ASTRONOMY AND ASTROPHYSICS↗

The Dark Energy Survey Supernova Program: Corrections on Photometry Due to Wavelength-dependent Atmospheric Effects

Abstract Wavelength-dependent atmospheric effects impact photometric supernova flux measurements for ground-based observations. We present corrections on supernova flux measurements from the Dark Energy Survey Supernova Program’s 5YR sample (DES-SN5YR) for differential chromatic refraction (DCR) and wavelength-dependent seeing, and we show their impact on the cosmological parameters w and Ω m . We use g − i colors of Type Ia supernovae to quantify astrometric offsets caused by DCR and simulate point-spread functions (PSFs) using the GalSIM package to predict the shapes of the PSFs with DCR and wavelength-dependent seeing. We calculate the magnitude corrections and apply them to the magnitudes computed by the DES-SN5YR photometric pipeline. We find that for the DES-SN5YR analysis, not accounting for the astrometric offsets and changes in the PSF shape cause an average bias of +0.2 mmag and −0.3 mmag, respectively, with standard deviations of 0.7 mmag and 2.7 mmag across all DES observing bands ( griz ) throughout all redshifts. When the DCR and seeing effects are not accounted for, we find that w and Ω m are lower by less than 0.004 ± 0.02 and 0.001 ± 0.01, respectively, with 0.02 and 0.01 being the 1 σ statistical uncertainties. Although we find that these biases do not limit the constraints of the DES-SN5YR sample, future surveys with much higher statistics, lower systematics, and especially those that observe in the u band will require these corrections as wavelength-dependent atmospheric effects are larger at shorter wavelengths. We also discuss limitations of our method and how they can be better accounted for in future surveys.

79 ASTRONOMY AND ASTROPHYSICS↗

THE NANOGRAV NINE-YEAR DATA SET: EXCESS NOISE IN MILLISECOND PULSAR ARRIVAL TIMES

Gravitational wave (GW) astronomy using a pulsar timing array requires high-quality millisecond pulsars (MSPs), correctable interstellar propagation delays, and high-precision measurements of pulse times of arrival. Here we identify noise in timing residuals that exceeds that predicted for arrival time estimation for MSPs observed by the North American Nanohertz Observatory for Gravitational Waves. We characterize the excess noise using variance and structure function analyses. We find that 26 out of 37 pulsars show inconsistencies with a white-noise-only model based on the short timescale analysis of each pulsar, and we demonstrate that the excess noise has a red power spectrum for 15 pulsars. We also decompose the excess noise into chromatic (radio-frequency-dependent) and achromatic components. Associating the achromatic red-noise component with spin noise and including additional power-spectrum-based estimates from the literature, we estimate a scaling law in terms of spin parameters (frequency and frequency derivative) and data-span length and compare it to the scaling law of Shannon and Cordes. We briefly discuss our results in terms of detection of GWs at nanohertz frequencies.

79 ASTRONOMY AND ASTROPHYSICS↗

Probing the Broad-Line Region and the Accretion Disk in the Lensed Quasars HE 0435-1223, WFI 2033-4723, and HE 2149-2745 Using Gravitational Microlensing

We use single-epoch spectroscopy of three gravitationally lensed quasars, HE 0435-1223, WFI 2033-4723, and HE 2149-2745, to study their inner structure (broad-line region [BLR] and continuum source). We detect microlensing-induced magnification in the wings of the broad emission lines of two of the systems (HE 0435-1223 and WFI 2033-4723). In the case of WFI 2033-4723, microlensing affects two “bumps” in the spectra that are almost symmetrically arranged on the blue (coincident with an Al iii emission line) and red wings of C iii]. These match the typical double-peaked profile that follows from disk kinematics. The presence of microlensing in the wings of the emission lines indicates the existence of two different regions in the BLR: a relatively small one with kinematics possibly related to an accretion disk, and another one that is substantially more extended and insensitive to microlensing. There is good agreement between the estimated size of the region affected by microlensing in the emission lines, r{sub s}=10{sub −7}{sup +15}√(M/M{sub ⊙}) lt-day (red wing of C iv in HE 0435-1223) and r{sub s}=11{sub −7}{sup +28}√(M/M{sub ⊙}) lt-day (C iii] bumps in WFI 2033-4723), and the sizes inferred from the continuum emission, r{sub s}=13{sub −4}{sup +5}√(M/M{sub ⊙}) lt-day (HE 0435-1223) and r{sub s}=10{sub −2}{sup +3}√(M/M{sub ⊙}) lt-day (WFI 2033-4723). For HE 2149-2745 we measure an accretion disk size r{sub s}=8{sub −5}{sup +11}√(M/M{sub ⊙}) lt-day. The estimates of p, the exponent of the size versus wavelength (r{sub s}∝λ{sup p}), are 1.2 ± 0.6, 0.8 ± 0.2, and 0.4 ± 0.3 for HE 0435-1223, WFI 2033-4723, and HE 2149-2745, respectively. In conclusion, the continuum microlensing amplitude in the three quasars and chromaticity in WFI 2033-4723 and HE 2149-2745 are below expectations for the thin-disk model. The disks are larger and their temperature gradients are flatter than predicted by this model.

79 ASTRONOMY AND ASTROPHYSICS↗