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At least 685 records · Page 38

Shrubs Strongly Influence Snow Properties in Two Subarctic Watersheds

Understanding changes in snow distribution in permafrost ecosystems is fundamental to predicting their response to future climate change. The expansion of tall shrubs into tundra ecosystems can trap snow and insulate permafrost ecosystems during the winter, but the overall insulation effect is dependent upon many ecosystem properties. To study shrub–snow–ground interactions, small temperature sensors were deployed at two research sites on the Seward Peninsula of Alaska, USA, during the 2019–2020 winter. Snow temperatures were used to extrapolate multiple metrics, including freezing n-factors, the snow insulation effect, snow cover duration, and the length of the snowmelt period. Statistical and spatial analysis showed that shrub patches were a dominant control on all snow metrics. Within shrub patches, average ground temperatures were 2.1°C warmer, snow persisted 50 days longer, snow insulation was double, and a longer, later spring snowmelt period occurred compared to nonshrubby areas. Site-level differences contributed relatively little to variation in snow metrics, indicating that shrub presence is an overarching driver of snow–ground interactions at the locations examined. Shrub expansion, which is anticipated under climate change, will strongly impact future permafrost distribution and Arctic energy, water, and carbon cycles through snow–shrub–ground feedbacks.

54 ENVIRONMENTAL SCIENCES↗

Structures of single-layer {beta}-sheet proteins evolved from {beta}-hairpin repeats

Free-standing single-layer beta-sheets are extremely rare in naturally occurring proteins, even though beta-sheet motifs are ubiquitous. Here we report the crystal structures of three homologous, single-layer, anti-parallel beta-sheet proteins, comprised of three or four twisted beta-hairpin repeats. The structures reveal that, in addition to the hydrogen bond network characteristic of beta-sheets, additional hydrophobic interactions mediated by small clusters of residues adjacent to the turns likely play a significant role in the structural stability and compensate for the lack of a compact hydrophobic core. These structures enabled identification of a family of secreted proteins that are broadly distributed in bacteria from the human gut microbiome and are putatively involved in the metabolism of complex carbohydrates. A conserved surface patch, rich in solvent-exposed tyrosine residues, was identified on the concave surface of the beta-sheet. These new modular single-layer beta-sheet proteins may serve as a new model system for studying folding and design of beta-rich proteins.

beta-hairpin repeats↗

The central dogma and cosmological horizons

The central dogma of black hole physics – which says that from the outside a black hole can be described in terms of a quantum system with exp(Area/4G N ) states evolving unitarily – has recently been supported by computations indicating that the interior of the black hole is encoded in the Hawking radiation of the exterior. In this paper, we probe whether such a dogma for cosmological horizons has any support from similar computations. The fact that the de Sitter bifurcation surface is a minimax surface (instead of a maximin surface) causes problems with this interpretation when trying to import calculations analogous to the AdS case. This suggests anchoring extremal surfaces to the horizon itself, where we formulate a two-sided extremization prescription and find answers consistent with general expectations for a quantum theory of de Sitter space: vanishing total entropy, an entropy of A/4G N when restricting to a single static patch, an entropy of a subregion of the horizon which grows as the region size grows until an island-like transition at half the horizon size when the entanglement wedge becomes the entire static patch interior, and a de Sitter version of the Hartman-Maldacena transition.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Entropy, entanglement and swampland bounds in DS/dS

We calculate the entanglement entropy of the de-Sitter (dS) static patch in the context of the DS/dS correspondence. Interestingly, we find that there exists a one parameter family of bulk minimal surfaces that all have the same area. Two of them have appeared earlier in the literature. All of them correctly calculate the dS entropy. One surface yields the entanglement between the two different CFTs that provide the holographic dual of the bulk DS geometry. The second surface describes the entanglement across the horizon in the boundary static patch. The other surfaces describe a mixture of these two concepts. We also show that in the presence of extra matter fields the former entanglement entropy always exceeds the dS entropy. We interpret this result in the context of entropy bounds in de Sitter space and the swampland program.

72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS↗

Coral and Seawater Metagenomes Reveal Key Microbial Functions to Coral Health and Ecosystem Functioning Shaped at Reef Scale

The coral holobiont is comprised of a highly diverse microbial community that provides key services to corals such as protection against pathogens and nutrient cycling. The coral surface mucus layer (SML) microbiome is very sensitive to external changes, as it constitutes the direct interface between the coral host and the environment. Here, we investigate whether the bacterial taxonomic and functional profiles in the coral SML are shaped by the local reef zone and explore their role in coral health and ecosystem functioning. The analysis was conducted using metagenomes and metagenome-assembled genomes (MAGs) associated with the coral Pseudodiploria strigosa and the water column from two naturally distinct reef environments in Bermuda: inner patch reefs exposed to a fluctuating thermal regime and the more stable outer reefs. The microbial community structure in the coral SML varied according to the local environment, both at taxonomic and functional levels. The coral SML microbiome from inner reefs provides more gene functions that are involved in nutrient cycling (e.g., photosynthesis, phosphorus metabolism, sulfur assimilation) and those that are related to higher levels of microbial activity, competition, and stress response. In contrast, the coral SML microbiome from outer reefs contained genes indicative of a carbohydrate-rich mucus composition found in corals exposed to less stressful temperatures and showed high proportions of microbial gene functions that play a potential role in coral disease, such as degradation of lignin-derived compounds and sulfur oxidation. The fluctuating environment in the inner patch reefs of Bermuda could be driving a more beneficial coral SML microbiome, potentially increasing holobiont resilience to environmental changes and disease.

59 BASIC BIOLOGICAL SCIENCES↗

Impact of Forest Canopy Structure on Buoyant Plume Dynamics During Wildland Fires

Heterogeneous forest canopies can generate complex turbulent structures, but in the presence of a fire plume, these interactions are not fully understood. This study investigates the influence of forest canopy heterogeneity on buoyant plume dynamics resulting from surface thermal anomalies representing wildland fires, utilizing Large Eddy Simulation (LES). The Parallelized Large-Eddy Simulation Model (PALM) was employed to simulate six canopy configurations: no canopy, homogeneous canopy, external plume-edge canopy, internal plume-edge canopy, 100 m gap canopy, and 200 m gap canopy. Each configuration was analyzed with and without a static surface heat flux patch of 5000 W ∙ m -2 , resulting in a resting buoyant plume. Simulations were conducted under three crosswind speeds: 0, 5, and 10 m ∙ s -1 . Results show that canopy structure significantly modifies plume behavior, mean flow, and turbulent kinetic energy (TKE) budgets. Plume updraft speed and tilt varied with canopy configuration and crosswind speed. Horizontal pressure gradients associated with plume-atmosphere interaction were modified based on the canopy configuration, resulting in varying crosswind speed reductions at the plume region. Strong momentum absorption was observed above the canopy for the crosswind cases, with the greatest enhancement in the gap canopies. Momentum injection from below the canopy due to the heat source was also observed, resulting in plume structure modulation based on canopy configuration. TKE was found to be the largest in the gap canopy configurations. TKE budget analysis revealed that buoyant production dominated over shear production. At the center of the heat patch, the gap canopy configurations showed enhanced buoyancy within the gap. These results improve our knowledge of fire-canopy-atmosphere interactions that can inform fire models on the impacts of canopy heterogeneity on plume dynamics and ember ejections.

54 ENVIRONMENTAL SCIENCES↗

Optimal Control Problem for Two-Layer Elastic Body with Crack

We establish the unique solvability of the equilibrium problem for a two-layer body. One layer contains a crack, whereas the second one is glued along its edge to the first layer in such a way that to cover one of the crack ends. In the case, where the second layer is a rigid plate, we show that the problem with a rigid patch is the limit of problems with elastic patches as the rigidity parameter tends to infinity. We also study the optimal control problem with the exterior forces acting on both layers taken for the control function.

36 MATERIALS SCIENCE↗

Single-atom materials boosting wearable orthogonal uric acid detection

Abstract Uric acid (UA) is a vital biomarker for the diagnosis and management of various health conditions, including cardiovascular diseases, gout, kidney disorders, metabolic syndrome, and wound healing. Despite significant advances in wearable sensor technology, challenges persist in developing wearable sensors that are capable of maintaining high sensitivity, selectivity, and stability. In this study, we present an epidermal sensing platform enhanced with single-atom materials (SAMs) designed for flexible and orthogonal electrochemical detection of UA. We designed and synthesized an SAM with Fe-N 5 active sites to boost the electrochemical sensing signals, integrating it with laser-engraved graphene (LEG) to fabricate a wearable SAM-based UA patch sensor. This design provides superior UA detection performance compared to sensors based on conventional nanomaterials. In addition, we enhanced the detection accuracy and range by using an orthogonal approach that combines direct oxidation through differential pulse voltammetry (DPV) along with parallel biocatalytic amperometric detection. The resulting SAM-based UA orthogonal sensor patch demonstrated exceptional performance in wearable applications through tests measuring sweat UA levels in subjects before and after consuming a purine-rich diet. Graphical Abstract

Ding, Shichao↗

Geological heterogeneity: Goal-oriented simplification of structure and characterization needs

Geological heterogeneity, i.e. the spatial variability of discrete hydrogeological units, is investigated in an aquifer analog of glacio-fluvial sediments to determine how such a geological structure can be simplified for characterization needs. Here, the aquifer analog consists of ten hydrofacies whereas the scarcity of measurements in typical field studies precludes such detailed spatial models of hydraulic properties. Of particular interest is the role of connectivity of the hydrofacies structure, along with its effect on the connectivity of mass transport, in site characterization for predicting early arrival times. Transport through three realizations of the aquifer analog is modeled with numerical particle tracking to ascertain the fast flow channel through which early arriving particles travel. Three simplification schemes of two-facies models are considered to represent the aquifer analogs, and the velocity within the fast flow channel is used to estimate the apparent hydraulic conductivity of the new facies. The facies models in which the discontinuous patches of high hydraulic conductivity are separated from the rest of the domain yield the closest match in early arrival times compared to the aquifer analog, but assuming a continuous high hydraulic conductivity channel connecting these patches yields underestimated early arrivals times within the range of variability between the realizations, which implies that the three simplification schemes could be advised but pose different implications for field measurement campaigns. Overall, the results suggest that the result of transport connectivity, i.e. early arrival times, within realistic geological heterogeneity can be conserved even when the underlying structural connectivity is modified.

58 GEOSCIENCES↗

Combining eddy-covariance and chamber measurements to determine the methane budget from a small, heterogeneous urban floodplain wetland park

Methane (CH 4 ) emissions and carbon uptake in temperate freshwater wetlands act in opposing directions in the context of global radiative forcing. Large uncertainties exist for the rates of CH 4 emissions making it difficult to determine the extent that CH 4 emissions counteract the carbon sequestration of wetlands. Urban temperate wetlands are typically small and feature highly heterogeneous land cover, posing an additional challenge to determining their CH 4 budget. The data analysis approach we introduce here combines two different CH 4 flux measurement techniques to overcome scale and heterogeneity problems and determine the overall CH 4 budget of a small, heterogeneous, urban wetland landscape. Temporally intermittent point measurements from non-steady-state chambers provided information about patch-level heterogeneity of fluxes, while continuous, high temporal resolution flux measurements using the eddy-covariance (EC) technique provided information about the temporal dynamics of the fluxes. Patch-level scaling parameterization was developed from the chamber data to scale eddy covariance data to a ‘fixed-frame’, which corrects for variability in the spatial coverage of the eddy covariance observation footprint at any single point in time. Finally, by combining two measurement techniques at different scales, we addressed shortcomings of both techniques with respect to heterogeneous wetland sites.

03 NATURAL GAS↗

Probing properties of the interfacial perimeter sites in TiO x /Au/SiO 2 with 2-propanol decomposition

The decomposition of 2-propanol was studied over SiO2, SiO2 with an overlayer of TiO2 (Ti/SiO2), Au/SiO2, and Au/SiO2 with an overlayer of TiO2 (Ti/[Au/SiO2]) at 170–190 °C. There was no reaction on SiO2. Propene was the only product on Ti/SiO2, and its rate of formation increased proportionally with the Ti content. Acetone was the major product (selectivity 65–99%) on all Au-containing catalysts. Its rate of formation also increased with Ti loading. In addition, small amounts of propene were also formed on Ti/[Au/SiO2] the rate of which increased with Ti loading. Characterization of the catalysts with N2 adsorption, STEM, DR-UV-vis spectroscopy, XPS, XANES and EXAFS suggested that the Ti formed an amorphous TiO2 overlayer on the catalyst. At high Ti loadings (4–5 wt.%), there were patches of thick porous TiO2 layer, and some microdomains of crystalline TiO2 could be detected. Au was present as 1–3 nm nanoparticles on all catalysts, before and after used in reaction. Only Lewis acid sites were detected based on results from pyridine adsorption, and their quantities increased with Ti loading. Based on the comparison of reaction rates, the dependence of the kinetics on 2-propanol partial pressure, the apparent activation energies, and the effect of co-feeding O2 among different catalysts, it was concluded that propene was formed on the TiO2 overlayer, acetone was formed primarily at the Au-TiO2 interfacial perimeter sites, and α-C-H bond breaking preceding acetone formation was more facile on Au at the interfacial site than other surface Au atoms. Implication of these results to the selective acetone formation in the oxidation of propane in the presence of a O2/H2 mixture was discussed.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Selective Catalytic Reduction of NO by NH 3 with WO 3 -TiO 2 Catalysts: Influence of Catalyst Synthesis Method

A series of supported WO 3 /TiO 2 catalysts was prepared by a new synthesis procedure involving co-precipitation of an aqueous TiO(OH) 2 and (NH 4 ) 10 W 12 O 41 *5H 2 O slurry under controlled pH conditions. The morphological properties, molecular structures, surface acidity and surface chemistry of the supported WO 3 /TiO 2 catalysts were determined with BET, in situ Raman, in situ IR and temperature-programmed surface reaction (TPSR) spectroscopy, respectively. Isotopic 18 O- 16 O exchange demonstrated that tungsten oxide was exclusively present as surface WO x species on the TiO 2 support with mono-oxo W=O coordination. In contrast to previous studies employing impregnation synthesis that found only surface one mono-oxo O=WO 4 site on TiO 2 , the co-precipitation procedure resulted in the formation of two distinct surface WO x species: mono-oxo O=WO 4 (~1010-1017 cm -1 ) on low defect density patches of TiO 2 and a second mono-oxo O=WO 4 (~983-986 cm -1 ) on high defect density patches of TiO 2 . The concentration of the second WO x surface species increases as a function of solution pH. Both surface WOx sites, however, exhibited the same NO/NH 3 SCR reactivity. The co-precipitated WO 3 -TiO 2 catalysts synthesized in alkaline solutions exhibited enhanced performance for the NO/NH 3 SCR reaction that is ascribed to the greater number of surface defects on the resulting TiO2 support. For the co-precipitated catalyst prepared at pH10, surface NH 4 + species on Br nsted acid sites were found to be more reactive than surface NH 3 * species on Lewis acid sites for SCR of NO with NH 3 .

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Basic Tilted Helix Bundle – A new protein fold in human FKBP25/FKBP3 and HectD1

Highlights: • We describe the structure of a novel fold in FKBP25 and HectD. • The new fold is named the Basic Tilted Helix Bundle (BTHB) domain. • A conserved basic surface patch is presented, suggesting a functional role. - Abstract: In this paper, we describe the structure of a N-terminal domain motif in nuclear-localized FKBP25{sub 1–73}, a member of the FKBP family, together with the structure of a sequence-related subdomain of the E3 ubiquitin ligase HectD1 that we show belongs to the same fold. This motif adopts a compact 5-helix bundle which we name the Basic Tilted Helix Bundle (BTHB) domain. A positively charged surface patch, structurally centered around the tilted helix H4, is present in both FKBP25 and HectD1 and is conserved in both proteins, suggesting a conserved functional role. We provide detailed comparative analysis of the structures of the two proteins and their sequence similarities, and analysis of the interaction of the proposed FKBP25 binding protein YY1. We suggest that the basic motif in BTHB is involved in the observed DNA binding of FKBP25, and that the function of this domain can be affected by regulatory YY1 binding and/or interactions with adjacent domains.

60 APPLIED LIFE SCIENCES↗

TMEM16F/ANO6, a Ca2+-activated anion channel, is negatively regulated by the actin cytoskeleton and intracellular MgATP

Highlights: • ANO6 current shows sluggish activation even with high [Ca{sup 2+}]{sub i}, and inactivation. • Actin cytoskeleton disruption accelerate both activation and inactivation of ANO6. • Intracellular MgATP further delays ANO6 activation, and prevent inactivation. • The effects of MgATP on ANO6 were overcome by actin inhibitor, cytochalasin-D. • State actin cytoskeleton is crucial for determining ANO6 activity in intact cells. Anoctamin 6 (ANO6/TMEM16F) is a recently identified membrane protein that has both phospholipid scramblase activity and anion channel function activated by relatively high [Ca{sup 2+}]{sub i}. In addition to the low sensitivity to Ca{sup 2+}, the activation of ANO6 Cl{sup −} conductance is very slow (>3–5 min to reach peak level at 10 μM [Ca{sup 2+}]{sub i}), with subsequent inactivation. In a whole-cell patch clamp recording of ANO6 current (I{sub ANO6,w-c}), disruption of the actin cytoskeleton with cytochalasin-D (cytoD) significantly accelerated the activation kinetics, while actin filament-stabilizing agents (phalloidin and jasplakinolide) commonly inhibited I{sub ANO6,w-c}. Inside-out patch clamp recording of ANO6 (I{sub ANO6,i-o}) showed immediate activation by raising [Ca{sup 2+}]{sub i}. We also found that intracellular ATP (3 mM MgATP in pipette solution) decelerated the activation of I{sub ANO6,w-c}, and also prevented the inactivation of I{sub ANO6,w-c}. However, the addition of cytoD still accelerated both activation and inactivation of I{sub ANO6,w-c}. We conclude that the actin cytoskeleton and intracellular ATP play major roles in the Ca{sup 2+}-dependent activation and inactivation of I{sub ANO6,w-c}, respectively.

60 APPLIED LIFE SCIENCES↗

Grassland disturbance increases monarch butterfly oviposition and decreases arthropod predator abundance

Many species of conservation concern depend on disturbance to create or maintain suitable habitat. We evaluated effects of disturbance on the eastern migratory monarch butterfly (Danaus plexippus L.), which has declined markedly in recent decades, primarily attributed to the loss of milkweed host plants from annual crop fields in the US Midwest. Currently, remaining milkweeds in this region primarily occur in perennial grasslands, where disturbance is infrequent, predatory arthropods are abundant, and seasonal patterns of plant phenology differ from crop fields. In a two-year study in Michigan, USA, we applied three treatments to 23 patches of common milkweed (Asclepias syriaca L.); one-third of each patch was left undisturbed, while the remaining thirds were mowed in either mid-June or mid-July, respectively, and allowed to regenerate. We subsequently measured effects on monarch oviposition, predator abundance, survival of sentinel eggs and larvae, and tested how milkweed phenology and aphid colonization—both of which are reset by disturbance—structure predation risk for immature monarchs. Monarchs laid more eggs on regenerating versus undisturbed stems under both mowing regimes. Predators were strongly suppressed by mowing treatments, requiring 2–4 weeks to recolonize milkweed after disturbance, and were more abundant on flowering or aphid-infested stems. We found no significant differences in monarch egg/larval survival, although it tended to be higher in mowed plots. Overall, monarchs laid more eggs on regenerating stems where their offspring may also experience enemy-free space. Future work should focus on testing grassland disturbance as a management tool to improve productivity of existing monarch breeding habitat.

54 ENVIRONMENTAL SCIENCES↗

Tuning electronic properties by oxidation-reduction reactions at graphene-ruthenium interface

Mass production of graphene is associated with the growth on catalysts used also in other chemical reactions. In this study, we exploit the oxidation-reduction to tailor the properties of single layer graphene domains with incorporated bi-layer patches on ruthenium. Using photoelectron spectromicroscopy techniques, we find that oxygen, intercalating under single layer and making it p-doped by the formation of Ru-O x , does not intercalate under the bilayer patches with n-doped upper layer, but decorates them under single layer surrounding creating lateral p-n junctions with chemical potential difference of 1.2 eV. O-reduction by thermal treatment in vacuum results in C-vacancy defects enhancing electronic coupling of remained graphene to Ru, whereas in H 2 , vacancy formation is suppressed. Also, for the domains below 15–25 μm size, after O-reduction in H 2 , graphene/Ru coupling is restored, while wrinkle pattern produced by O-intercalation is irreversible and can trap reaction products between the wrinkles and Ru surface step edges. In fact, in certain regions of bigger domains, the products, containing H 2 O and/or its fragments, remain at the interface, making graphene decoupled and undoped.

36 MATERIALS SCIENCE↗

Geometric prior of multi-resolution yielding manifolds and the local closest point projection for nearly non-smooth plasticity

Elastoplasticity models often introduce a scalar-valued yield function to implicitly represent the boundary between elastic and plastic material states. Here this paper introduces a new alternative where the yield envelope is represented by a manifold of which the topology and the geometry are learned from a set of data points in a parametric space (e.g. principal stress space, π-plane). Here, deep geometric learning enables us to reconstruct a highly complex yield envelope by breaking it down into multiple coordinate charts. The global atlas that consists of these coordinate charts in return allows us to represent the yield surface via multiple overlapping patches, each with a specific local parametrization. This setup provides several advantages over the classical implicit function representation approach. For instance, the availability of coordinate charts enables us to introduce an alternative stress integration algorithm where the trial stress may project directly on a local patch and hence circumvent the issues related to non-smoothness and the lack of convexity of yield surfaces. Meanwhile, the local parametric approach also enables us to predict hardening/softening locally in the parametric space, even without complete knowledge of the yield surface. Comparisons between the classical yield function approach on the non-smooth plasticity and anisotropic cam-clay plasticity model are provided to demonstrate the capacity of the models for highly precise yield surface and the feasibility of the implementation of the learned model in the local stress integration algorithm.

42 ENGINEERING↗

INGRID: An interactive grid generator for 2D edge plasma modeling

A fusion boundary-plasma domain is defined by axisymmetric magnetic surfaces where the geometry is often complicated by the presence of one or more X-points; and modeling boundary plasmas usually relies on computational grids that account for the magnetic field geometry. The new grid generator INGRID (Interactive Grid Generator) presented in this work is a Python-based code for calculating grids for fusion boundary plasma modeling, for a variety of configurations with one or two X-points in the domain. INGRID first performs partitioning over the domain consisting of a small number of patches conforming to the magnetic field and wall geometry; then it generates a subgrid on each of the patches and joins them into a global grid. This domain partitioning strategy makes possible a uniform treatment of various configurations with one or two X-points in the domain. This includes single-null, double-null, and other configurations with two X-points in the domain. The INGRID design allows generating grids either interactively, via a parameter-file driven GUI, or using a non-interactive script-controlled workflow. Results of testing demonstrate that INGRID is a flexible, robust, and user-friendly grid-generation tool for fusion boundary-plasma modeling.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗