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At least 685 records · Page 38

Planning green infrastructure placement based on projected precipitation data

Continued urbanization has led to tremendous changes on the landscape. These changes have exacerbated the effects of extreme climatic events such as flooding because of constrained water infiltration and increased surface flow. Typical runoff control measures involve sophisticated gray infrastructure that guide excess surface flow into storage and disposal sites. In a dynamic climate system, these measures are not sustainable since they cannot be easily modified to accommodate large volumes of runoff. Green Infrastructure (GI) is an adaptable technique that can be used to minimize runoff, in addition to offering an array of additional benefits (urban heat regulation, aesthetics, improved air quality etc.). Strategic placement of GI is key to achieving maximum utility. While physical site characteristics play a major role in determining suitable GI placement sites, knowledge of future precipitation patterns is crucial to ensure successful flood mitigation. Here, suitable GI sites within the city of Knoxville, Tennessee, were determined based on potential impact of an extreme flood event as indicated by site characteristics. Then, the relative potential likelihood of a flood event was determined based on projected precipitation data and knowledge of existing flood zones. By combining potential impact with likelihood information, low, medium, and high priority GI implementation sites were established. Results indicate that high priority sites are in the central parts of the city with priority decreasing outward. The GI prioritization scheme presented here, offers valuable guidance to city planners and policy makers who wish to exploit the GI approach for flood mitigation.

54 ENVIRONMENTAL SCIENCES↗

CreA-mediated repression of gene expression occurs at low monosaccharide levels during fungal plant biomass conversion in a time and substrate dependent manner

Carbon catabolite repression enables fungi to utilize the most favourable carbon source in the environment, and is mediated by a key regulator, CreA, in most fungi. CreA-mediated regulation has mainly been studied at high monosaccharide concentrations, an uncommon situation in most natural biotopes. In nature, many fungi rely on plant biomass as their major carbon source by producing enzymes to degrade plant cell wall polysaccharides into metabolizable sugars. To determine the role of CreA when fungi grow in more natural conditions and in particular with respect to degradation and conversion of plant cell walls, we compared transcriptomes of a creA deletion and reference strain of the ascomycete Aspergillus niger during growth on sugar beet pulp and wheat bran. Transcriptomics, extracellular sugar concentrations and growth profiling of A. niger on a variety of carbon sources, revealed that also under conditions with low concentrations of free monosaccharides, CreA has a major effect on gene expression in a strong time and substrate composition dependent manner. In addition, we compared the CreA regulon from five fungi during their growth on crude plant biomass or cellulose. It showed that CreA commonly regulated genes related to carbon metabolism, sugar transport and plant cell wall degrading enzymes across different species. We therefore conclude that CreA has a crucial role for fungi also in adapting to low sugar concentrations as occurring in their natural biotopes, which is supported by the presence of CreA orthologs in nearly all fungi.

59 BASIC BIOLOGICAL SCIENCES↗

Analysis of Double-Output CLL Resonant Converter for All-Electric UAV Applications

This paper presents a double-output resonant converter with high-frequency isolation for unmanned aerial vehicle (UAV) applications. The proposed topology is developed by using a full-bridge resonant inverter and double-output half-bridge active rectifiers. For the control system, a phase-shifted pulse width modulation (PS-PWM) method is utilized to adjusts the power flow among the output rectifiers in a wide voltage range regulation with a central control. The theoretical analysis of the converter is explored using the system model under different load conditions with constant frequency. The proposed system is validated with experimental results using 60 V input source and generating 0 - 100 V in each output at a full power of 500 W with 96% maximum efficiency in the laboratory experimental setup.

Asa, Erdem↗

Identification of a p-Coumarate Degradation Regulon in Rhodopseudomonas palustris by Xpression, an Integrated Tool for Prokaryotic RNA-Seq Data Processing

High-throughput sequencing of cDNA prepared from RNA, an approach known as RNA-seq, is coming into increasing use as a method for transcriptome analysis. Despite its many advantages, widespread adoption of the technique has been hampered by a lack of easy-to-use, integrated, open-source tools for analyzing the nucleotide sequence data that are generated. Here we describe Xpression, an integrated tool for processing prokaryotic RNA-seq data. The tool is easy to use and is fully automated. It performs all essential processing tasks, including nucleotide sequence extraction, alignment, quantification, normalization, and visualization. Importantly, Xpression processes multiplexed and strand-specific nucleotide sequence data. It extracts and trims specific sequences from files and separately quantifies sense and antisense reads in the final results. Outputs from the tool can also be conveniently used in downstream analysis. In this paper, we show the utility of Xpression to process strand-specific RNA-seq data to identify genes regulated by CouR, a transcription factor that controls p -coumarate degradation by the bacterium Rhodopseudomonas palustris.

59 BASIC BIOLOGICAL SCIENCES↗

Opportunities and Challenges for Water and Wastewater Industries to Provide Exchangeable Services

Water and wastewater treatment plants and distribution systems use significant amounts of energy, around 2 - 4% of the total electricity used in the US, and their energy use is projected to increase as populations increase and regulations become more stringent. Water and wastewater systems have largely been disconnected from the electric utilities' efforts to improve energy efficiency and provide energy efficiency and provide grid services, likely because their core mission is to provide clean water and treated wastewater. Energy efficiency has slowly crept into the water and wastewater industry as the economic benefit has become more apparent, but there is still potential for significant improvement. Some of the larger, more progressive water utilities are starting to consider providing grid services; however, it remains a foreign concept to many. This report explores intrinsic mechanisms by which the water and wastewater industries can provide exchangeable services, the benefit to the parties involved, and the barriers to implementation. It also highlights relevant case studies and next steps. Although opportunities for increasing process efficiencies are certainly available, this report focuses on the exchangeable services that water and wastewater loads can provide to help maintain grid reliability, keep overall costs down, and increase the penetration of distributed renewables on the electric grid. These services have potential to provide water utilities additional value streams, using existing equipment with modest or negligible upgrade cost.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Hierarchical Distributed Voltage Regulation in Networked Autonomous Grids

We propose a novel algorithm to solve optimal power flow (OPF) that aims at dispatching controllable distributed energy resources (DERs) for voltage regulation at minimum cost. The proposed algorithm features unprecedented scalability to large distribution networks by utilizing an information structure based on networked autonomous grids (AGs). Specifically, each AG is a subtree of a large distribution network that has a tree topology. The topology and line parameters of each AG are known only to a regional coordinator (RC) that is responsible for communicating with and dispatching the DERs within this AG. The reduced network, where each AG is treated as a node, is managed by a central coordinator (CC), which knows the topology and line parameters of the reduced network only and communicates with all the RCs. We jointly explore this information structure and the structure of the linearized distribution power flow (LinDistFlow) model to derive a hierarchical, distributed implementation of the primal-dual gradient algorithm that solves the OPF. The proposed implementation significantly reduces the computation burden compared to the centrally coordinated implementation of the primal-dual algorithm. Numerical results on a 4,521-node test feeder show that the proposed hierarchical distributed algorithm can achieve an improvement of more than tenfold in the speed of convergence compared to the centrally coordinated primal-dual algorithm.

Zhou, Xinyang↗

Ducted Fuel Injection Provides Consistently Lower Soot Emissions in Sweep to Full-Load Conditions

Earlier studies have proven how ducted fuel injection (DFI) substantially reduces soot for low- and mid-load conditions in heavy-duty engines, without significant adverse effects on other emissions. Nevertheless, no comprehensive DFI study exists showing soot reductions at high- and full-load conditions. This study investigated DFI in a single-cylinder, 1.7-L, optical engine from low- to full-load conditions with a low-net-carbon fuel consisting of 80% renewable diesel and 20% biodiesel. Over the tested load range, DFI reduced engine-out soot by 38.1–63.1% compared to conventional diesel combustion (CDC). This soot reduction occurred without significant detrimental effects on other emission types. Thus, DFI reduced the severity of the soot–NO x tradeoff at all tested conditions. While DFI delivered considerable soot reductions in thepresent study, previous DFI studies at low- and mid-load conditions delivered larger soot reductions (>90%) compared to CDC operation at the same conditions. Therefore, the DFI configuration used here has been deemed nonoptimal (in terms of parameters such as the injector-spray and piston geometries), and several improvements are recommended for future studies with high-load DFI. Further, these improvements include employing better spray-duct alignment, a deeper piston bowl with a smaller injector umbrella angle, and a fuel injector that opens and closes faster. The study also suggests future research to make DFI ready for commercialization, such as metal-engine tests to ensure desirable DFI performance over an engine’s complete speed/load map. Overall, this study supports the continued development and commercialization of DFI to meet upcoming emissions regulations for heavy-duty vehicles. Specifically, multicylinder engine experiments and CFD simulations should be utilized to optimize the performance and clarify the full potential of DFI.

33 ADVANCED PROPULSION SYSTEMS↗

Q-FANSTM for general aviation aircraft

Continued growth of general aviation over the next 10 to 15 years is dependent on continuing improvement in aircraft safety, utility, performance and cost. Moreover, these advanced aircraft will need to conform to expected government regulations controlling propulsion system emissions and noise levels. An attractive compact low noise propulsor concept, the Q-FANTM when matched to piston, rotary combustion, or gas turbine engines opens up the exciting prospect of new, cleaner airframe designs for the next generation of general aviation aircraft which will provide these improvements and meet the expected noise and pollution restriction of the 1980 time period. New Q-FAN methodology which was derived to predict Q-FAN noise, weight and cost is presented. Based on this methodology Q-FAN propulsion system performance, weight, noise, and cost trends are discussed. Then the impact of this propulsion system type on the complete aircraft is investigated for several representative aircraft size categories. Finally, example conceptual designs for Q-FAN/engine integration and aircraft installations are presented.

Worobel, R.↗

The preliminary design of an orbiting observatory - The Space Telescope

The systems and subsystems of the Space Telescope proposed for an orbiting observatory to be launched by the Space Shuttle in the 1980s are described. The structural design is simple and based on existing technologies for high reliability. Provisions are made for on-orbit servicing and maintenance. All deployable appendages are designed for manual override to insure retrieval and return of the Space Telescope to earth for major refurbishing, which should occur at intervals of not less than six years. Low performance risk, passive techniques are used in the thermal control subsystem to provide a cold-biased design with thermostatically controlled heaters to adjust temperature. The electrical power system utilizes NASA Standard Hardware - 50 ampere hour nickel-cadmium battery cells, a standard power regulator unit, and a flight-proven flexible rollup solar array with high-efficiency (12.5 per cent) silicon solar cells. The communication subsystem is designed for compatibility with the Tracking and Data Relay Satellite System. The pointing control system will have a maximum line of sight variation of 0.007 arc seconds. The telescope optics are a Ritchey-Chretien version of the Cassegrain configuration.

Timmons, K. P.↗

Space Tissue Loss Configuration B (STL-B)

The goal of these experiments was to determine the effect of microgravity on the early development of the fish medaka. There were two objectives for this flight series. The primary objective was to assess the effects of microgravity on different stages of development and to ascertain whether the relevant developmental questions can be addressed at the gross morphological level or if the issues involve more subtle questions about regulation at the molecular and cellular levels. The secondary objective was the assessment of the utility of flight hardware with the capabilities to perform embryological studies. We have been able to take advantage of the flight testing phase of the STL-B hardware to also study the effects of microgravity on the early development of the fish, Medaka. Our initial studies involved monitoring the early Medaka development and raising flight embryos for breeding. Images of the developing embryos were collected either via video which was either taken by the astronauts or broadcast to Earth. Sample video images were digitized and stored on a hard drive resident within the on-board STL-B unit. Embryos were fixed at specific intervals, returned to Earth and are being analyzed for the timing and location of molecular events associated with controlling the morphological pattern for the onset of adult structures.

Source record↗

Development of the Fish Medaka in Microgravity

The goal of these experiments was to determine the effect of microgravity on the early development of the fish medaka. There were two objectives for this flight series. The primary objective was to assess the effects of microgravity on different stages of development and to ascertain whether the relevant developmental questions can be addressed at the gross morphological level or if the issues involve more subtle questions about regulation at the molecular and cellular levels. The secondary objective was the assessment of the utility of flight hardware with the capabilities to perform embryological studies. We have been able to take advantage of the flight testing phase of the STL-B hardware to also study the effects of microgravity on the early development of the fish, Medaka. Our initial studies involved monitoring the early Medaka development and raising flight embryos for breeding. Images of the developing embryos were collected either via video which was either taken by the astronauts or broadcast to Earth. Sample video images were digitized and stored on a hard drive resident within the on-board STL-B unit. Embryos were fixed at specific intervals, returned to Earth and are being analyzed for the timing and location of molecular events associated with controlling the morphological pattern for the onset of adult structures.

Wolgemuth, Debra J.↗

NASA Office of Small and Disadvantaged Business Utilization

The Office of Small and Disadvantaged Business Utilization (OSDBU) within NASA promotes the utilization of small, disadvantaged, and women-owned small businesses in compliance with Federal laws, regulations, and policies. We assist such firms in obtaining contracts and subcontracts with NASA and its prime contractors. The OSDBU also facilitates the participation of small businesses in NASA's technology transfer and commercialization activities. Our driving philosophy is to consider small businesses as our products. Our customers are the NASA Enterprises, Field Centers, Functional Staff Offices, major prime contractors, and other large institutions. We hone the skills of our products to make them marketable to our customers in the performance of NASA missions.

Source record↗

SiC Multi-Chip Power Modules as Power-System Building Blocks

The term "SiC MCPMs" (wherein "MCPM" signifies "multi-chip power module") denotes electronic power-supply modules containing multiple silicon carbide power devices and silicon-on-insulator (SOI) control integrated-circuit chips. SiC MCPMs are being developed as building blocks of advanced expandable, reconfigurable, fault-tolerant power-supply systems. Exploiting the ability of SiC semiconductor devices to operate at temperatures, breakdown voltages, and current densities significantly greater than those of conventional Si devices, the designs of SiC MCPMs and of systems comprising multiple SiC MCPMs are expected to afford a greater degree of miniaturization through stacking of modules with reduced requirements for heat sinking. Moreover, the higher-temperature capabilities of SiC MCPMs could enable operation in environments hotter than Si-based power systems can withstand. The stacked SiC MCPMs in a given system can be electrically connected in series, parallel, or a series/parallel combination to increase the overall power-handling capability of the system. In addition to power connections, the modules have communication connections. The SOI controllers in the modules communicate with each other as nodes of a decentralized control network, in which no single controller exerts overall command of the system. Control functions effected via the network include synchronization of switching of power devices and rapid reconfiguration of power connections to enable the power system to continue to supply power to a load in the event of failure of one of the modules. In addition to serving as building blocks of reliable power-supply systems, SiC MCPMs could be augmented with external control circuitry to make them perform additional power-handling functions as needed for specific applications: typical functions could include regulating voltages, storing energy, and driving motors. Because identical SiC MCPM building blocks could be utilized in a variety of ways, the cost and difficulty of designing new, highly reliable power systems would be reduced considerably. Several prototype DC-to-DC power-converter modules containing SiC power-switching devices were designed and built to demonstrate the feasibility of the SiC MCPM concept. In anticipation of a future need for operation at high temperature, the circuitry in the modules includes high-temperature inductors and capacitors. These modules were designed to be stacked to construct a system of four modules electrically connected in series and/or parallel. The packaging of the modules is designed to satisfy requirements for series and parallel interconnection among modules, high power density, high thermal efficiency, small size, and light weight. Each module includes four output power connectors two for serial and two for parallel output power connections among the modules. Each module also includes two signal connectors, electrically isolated from the power connectors, that afford four zones for signal interconnections among the SOI controllers. Finally, each module includes two input power connectors, through which it receives power from an in-line power bus. This design feature is included in anticipation of a custom-designed power bus incorporating sockets compatible with snap-on type connectors to enable rapid replacement of failed modules.

Lostetter, Alexander↗

The Impact of Transformer Winding Connections of A Grid-Connected PV on Voltage Quality Improvement

In this paper, the high-power PV plant is connected to the weak grid by means of a three-phase power transformer. The selection of transformer winding connection is critical especially when the PV inverter has a reactive power controller. In general, transformer winding connection can be arranged in star-star (with neutral earthed) or star-delta. The reactive power controller supports voltage regulation of the power system particularly under transient faults. Its control strategy is based on utilizing the grid currents to make a three-phase reactive unbalanced current with a small gain. The gain is determined by the system impedance. Simulation results exhibit that the control strategy works very well particularly under disturbance conditions when the transformer winding connection is star-star with both neutrals grounded. The power quality in terms of the voltage quality is improved.

14 SOLAR ENERGY↗

Controlling Air Conditioners for Frequency Regulation: A Real-World Example

Even though thermostatically controlled loads like air conditioners present a great potential for providing ancillary services to the electric power grid, the practical challenges associated with their real-time coordination have not received the necessary attention. In this work, we present a nondisruptive load control application, specifically, we demonstrate how real residential air conditioners can provide frequency regulation. Aggregate power adjustment is achieved by modifying the ON/OFF modes of the air conditioners. To account for both single and multi-zone houses, we extend the currently available techniques and develop an approach that can be used for controlling aggregations that include both types of houses. A discussion of the practical challenges encountered in our field experiments is provided, along with the hardware and software approaches we developed to circumvent them. Here, we argue that limitations of current thermostat APIs introduce significant challenges and are an impediment to widespread adoption of fast load control applications.

24 POWER TRANSMISSION AND DISTRIBUTION↗

Mutations in global regulators lead to metabolic selection during adaptation to complex environments

Adaptation to ecologically complex environments can provide insights into the evolutionary dynamics and functional constraints encountered by organisms during natural selection. Unlike adaptation to a single limiting resource, adaptation to a new environment with abundant and varied resources can be difficult to achieve by small incremental changes since many mutations are required to achieve even modest gains in fitness. Since changing complex environments are quite common in nature, we investigated how such an epistatic bottleneck can be avoided to allow rapid adaptation. We show that adaptive mutations arise repeatedly in independently evolved populations in the context of greatly increased genetic and phenotypic diversity. We go on to show that weak selection requiring substantial metabolic reprogramming can be readily achieved by mutations in the global response regulator arcA and the stress response regulator rpoS . We identified 46 unique single-nucleotide variants of arcA and 18 mutations in rpoS , nine of which resulted in stop codons or large deletions, suggesting that a subtle modulation of ArcA function and knockouts of rpoS are largely responsible for the metabolic shifts leading to adaptation. These mutations allow a higher order “metabolic selection” that eliminates epistatic bottlenecks, which could occur when many changes would be required. Proteomic and carbohydrate analysis of adapting E. coli populations revealed an up-regulation of enzymes associated with the TCA cycle and amino acid metabolism and an increase in the secretion of putrescine. The overall effect of adaptation across populations is to redirect and efficiently utilize uptake and catabolism of abundant amino acids. Concomitantly, there is a pronounced spread of more ecologically limited strains that results from specialization through metabolic erosion. Remarkably, the global regulators arcA and rpoS can provide a “one-step” mechanism of adaptation to a novel environment, which highlights the importance of global resource management as a powerful strategy to adaptation.

59 BASIC BIOLOGICAL SCIENCES↗

Next-Gen 3 : Sequencing, Modeling, and Advanced Biofuels - Final Technical Report

Successful, scalable implementation of biofuels is dependent on the efficient and near complete utilization of diverse biomass sources. One approach is to utilize the large recalcitrant biomass fraction (or any organic waste stream) through the thermochemical conversion of organic compounds to syngas, a mixture of carbon monoxide (CO), carbon dioxide (CO 2 ), and hydrogen (H 2 ), which can subsequently be metabolized by acetogenic microorganisms to produce next-gen biofuels. The goal of this proposal was to advance the development of the acetogen Clostridium ljungdahlii as a chassis organism for next-gen biofuel production from cheap, renewable sources and to detail the interconnectivity of metabolism, energy conservation, and regulation of acetogens using next-gen sequencing and next-gen modeling. To achieve this goal we determined optimization of carbon and energy utilization through differential translational efficiency in C. ljungdahlii. Furthermore, we reconstructed a next-generation model of all major cellular processes, such as macromolecular synthesis and transcriptional regulation and deployed this model to predicting proteome allocation, overflow metabolism, and metal requirements in this model acetogen. In addition we explored the evolutionary significance of tRNA operon structure using the next-gen model and determined the optimal operon structure for bioproduction. Our study substantially enhanced the knowledgebaase for chemolithoautotrophs and their potential for advanced biofuel production. It provides next-generation modeling capability, offer innovative tools for genome-scale engineering, and provide novel methods to utilize next-generation models for the design of tunable systems that produce commodity chemicals from inexpensive sources.

09 BIOMASS FUELS↗

Challenges of SLOWPOKE-2 HEU Irradiated Fuel Transportation from Canada to USA - 19123(2)

The SLOWPOKE-2 (Safe Low-Power Kritical Experiment) was designed by AECL (Atomic Energy of Canada Limited) in the late 1960's. The core consists of HEU (highly enriched uranium) fuel with an enrichment of 93%. The fuel cage typically has approximately 300 fuel pins assembled in a triangular pitch between the two plates. The fuel pins are made from uranium-aluminum alloy cladded with aluminum. The SLOWPOKE-2 reactor is rated at a nominal 20 kW. with varying neutron flux, maximum being 1 E12 n/cm{sup 2}/s. The mass of the fuel cage is 5.06 kg. The life of the core can be extended by adding layers of beryllium reflector. The transportation flask for the irradiated fuel is a Type B(U)F. The shipment classification is UN3328 Class 7. To date, 4 SLOWPOKE-2 reactors have been decommissioned. The fuel from all these reactors was transported to the U.S. The CNSC (Canadian Nuclear Safety Commission) joint Canada-United States guide for approval of Type B(U) and fissile material transportation packages (RD-364) satisfies the requirements stipulated in International Atomic energy Agency (IAEA) TS-R-1 1996 edition (revised) and is acceptable to the DOT (Department of Transportation) and NRC (Nuclear Regulatory Commission) for complying with U.S. code of federal regulations 10 CFR Part 71. The export license is issued by the CNSC. The transportation licence is issued by CNSC after approval from TC (Transport Canada) and also by DOT after approval from NRC. The final home of the HEU SLOWPOKE-2 irradiated fuel is in Savannah River Site of DOE (Department of Energy). The transportation licence requires that the Type B(U)F flask is structurally sound in case of a crash. The US DOT provides a CAC (Competent Authority Certificate) which describes the irradiated fuel specifications that can be transported in the designated flask. The U.S. DOT also requires a leak test certification in accordance with ANSI N14.5-2014 to verify a leakage rate not to exceed 1 E-7 std-cm{sup 3}/s. As most SLOWPOKE-2 facilities are not located on the existing approved routes for nuclear fuel transportation, a route survey needs to be performed and submitted to the NRC. The route survey report is assessed by the NRC and issues a route approval for the transportation of the fissile material. This technical paper will discuss the detailed processes required to obtain approvals in order to transport the HEU SLOWPOKE-2 irradiated fuel from Canada to U.S. based upon recent experiences of decommissioning SLOWPOKE-2 reactors in Canada. It will provide details on the requirements of various regulators in Canada and the U.S. that govern and regulate this shipment including transportation, safety and security. It will also present lessons learned from previous experiences that can be utilized for best practice on other similar projects. (authors)

12 MANAGEMENT OF RADIOACTIVE AND NON-RADIOACTIVE W↗