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At least 685 records · Page 38

Renewable Diesel from Algal Lipids: An Integrated Baseline for Cost, Emissions, and Resource Potential from a Harmonized Model

The U.S. Department of Energy's Biomass Program has begun an initiative to obtain consistent quantitative metrics for algal biofuel production to establish an 'integrated baseline' by harmonizing and combining the Program's national resource assessment (RA), techno-economic analysis (TEA), and life-cycle analysis (LCA) models. The baseline attempts to represent a plausible near-term productionscenario with freshwater microalgae growth, extraction of lipids, and conversion via hydroprocessing to produce a renewable diesel (RD) blendstock. Differences in the prior TEA and LCA models were reconciled (harmonized) and the RA model was used to prioritize and select the most favorable consortium of sites that supports production of 5 billion gallons per year of RD. Aligning the TEA and LCAmodels produced slightly higher costs and emissions compared to the pre-harmonized results. However, after then applying the productivities predicted by the RA model (13 g/m2/d on annual average vs. 25 g/m2/d in the original models), the integrated baseline resulted in markedly higher costs and emissions. The relationship between performance (cost and emissions) and either productivity or lipidfraction was found to be non-linear, and important implications on the TEA and LCA results were observed after introducing seasonal variability from the RA model. Increasing productivity and lipid fraction alone was insufficient to achieve cost and emission targets; however, combined with lower energy, less expensive alternative technology scenarios, emissions and costs were substantiallyreduced.

09 BIOMASS FUELS↗

Determination of Total Lipids as Fatty Acid Methyl Esters: Laboratory Analytical Procedure (LAP)

This Laboratory Analytical Procedure (LAP) covers the determination of total lipids expressed as fatty acid methyl esters (FAME). Results are reported as the percent FAME content based on the dry weight of the sample. The procedure is based on a whole biomass transesterification procedure of lipids to FAME, which eliminates the need for extraction and therefore is able to access all fatty acids in the biomass and represent an accurate reflection of the biofuels potential.

09 BIOMASS FUELS↗

Real Space and Time Imaging of Collective Headgroup Dipole Motions in Zwitterionic Lipid Bilayers

Lipid bilayers are supramolecular structures responsible for a range of processes, such as transmembrane transport of ions and solutes, and sorting and replication of genetic materials, to name just a few. Some of these processes are transient and currently, cannot be visualized in real space and time. Here, we developed an approach using 1D, 2D, and 3D Van Hove correlation functions to image collective headgroup dipole motions in zwitterionic phospholipid bilayers. We show that both 2D and 3D spatiotemporal images of headgroup dipoles are consistent with commonly understood dynamic features of fluids. However, analysis of the 1D Van Hove function reveals lateral transient and re-emergent collective dynamics of the headgroup dipoles—occurring at picosecond time scales—that transmit and dissipate heat at longer times, due to relaxation processes. At the same time, the headgroup dipoles also generate membrane surface undulations due a collective tilting of the headgroup dipoles. A continuous intensity band of headgroup dipole spatiotemporal correlations—at nanometer length and nanosecond time scales—indicates that dipoles undergo stretching and squeezing elastic deformations. Importantly, the above mentioned intrinsic headgroup dipole motions can be externally stimulated at GHz-frequency scale, enhancing their flexoelectric and piezoelectric capabilities (i.e., increased conversion efficiency of mechanical energy into electric energy). In conclusion, we discuss how lipid membranes can provide molecular-level insights about biological learning and memory, and as platforms for the development of the next generation of neuromorphic computers.

59 BASIC BIOLOGICAL SCIENCES↗

Cationic Lipid-Nucleic Acid Complexes for Gene Delivery And Silencing: Pathways And Mechanisms for Plasmid Dna And Sirna

Motivated by the promises of gene therapy, there is great interest in developing non-viral lipid-based vectors for therapeutic applications due to their low immunogenicity, low toxicity, ease of production, and the potential of transferring large pieces of DNA into cells. In fact, cationic liposome (CL) based vectors are among the prevalent synthetic carriers of nucleic acids (NAs) currently used in gene therapy clinical trials worldwide. These vectors are studied both for gene delivery with CL-DNA complexes and gene silencing with CL-siRNA (short interfering RNA) complexes. However, their transfection efficiencies and silencing efficiencies remain low compared to those of engineered viral vectors. This reflects the currently poor understanding of transfection-related mechanisms at the molecular and self-assembled levels, including a lack of knowledge about interactions between membranes and double stranded NAs and between CL-NA complexes and cellular components. In this review we describe our recent efforts to improve the mechanistic understanding of transfection by CL-NA complexes, which will help to design optimal lipid-based carriers of DNA and siRNA for therapeutic gene delivery and gene silencing.

59 BASIC BIOLOGICAL SCIENCES↗

Algal Lipids and Omega-3 Production via Autotrophic and Heterotrophic Pathways at Cellana?s Kona Demonstration Facility, Hawaii

Cellana?s Kona Demonstration Facility (KDF) is a 2.5 hectare facility, with 17,000 sq. ft. under roof and 1 hectare of cultivation systems. KDF is designed to execute and support all stages of the production process at pilot scale, from cultivation through extraction. Since Feb. 2009, KDF has been producing up to 0.7MT dry weight of algal biomass per month, while at the same time optimizing processes of cultivation, harvesting, dewatering and extraction. The cultivation system at KDF uses ALDUO? technology, a hybrid system of photobioreactors (PBRs) and open ponds. All fluid transfers related to KDF cultivation and harvesting processes are operated and monitored by a remote Process-Control System. Fluid transfer data, together with biochemical data, enable the mass balance calculations necessary to measure productivity. This poster summarizes methods to improve both biomass and lipids yield by 1) alleviating light limitation in open ponds, 2) de-oxygenation and 3) heterotrophic lipid production for post-harvesting cultures.

09 BIOMASS FUELS↗

Amphiphilic Nanoparticles Control the Growth and Stability of Lipid Bilayers with Open Edges

Molecular amphiphiles self-assemble in polar media to form ordered structures such as micelles and vesicles essential to a broad range of industrial and biological processes. Some of these architectures such as bilayer sheets, helical ribbons, and hollow tubules are potentially useful but inherently unstable owing to the presence of open edges that expose the hydrophobic bilayer core. Here, we describe a strategy to stabilize open bilayer structures using amphiphilic nanoparticle surfactants that present mixtures of hydrophilic and hydrophobic ligands on their surface. We observe that these particles bind selectively to the open edge of bilayer membranes to stabilize otherwise transient amphiphile assemblies. We show how such particles can precisely control the size of lipid tubules, how they can inhibit the formation of undesirable assemblies such as gallstone precursors, and how they can stabilize free-floating lipid microdiscs.

36 MATERIALS SCIENCE↗

ADP‐Induced Ca 2+ Signaling and Proliferation of Rat Ventricular Myofibroblasts Depend on Phospholipase C‐Linked TRP Channels Activation Within Lipid Rafts

Nucleotides released during heart injury affect myocardium electrophysiology and remodeling through P2 purinoceptors activation in cardiac myofibroblasts. ATP and UTP endorse [Ca 2+ ] i accumulation and growth of DDR‐2/α‐SMA‐expressing myofibroblasts from adult rat ventricles via P2Y 4 and P2Y 2 receptors activation, respectively. Ventricular myofibroblasts also express ADP‐sensitive P2Y 1 , P2Y 12 , and P2Y 13 receptors as demonstrated by immunofluorescence confocal microscopy and western blot analysis, but little information exists on ADP effects in these cells. ADP (0.003–3 mM) and its stable analogue, ADPßS (100 μM), caused fast [Ca 2+ ] i transients originated from thapsigargin‐sensitive internal stores, which partially declined to a plateau sustained by capacitative Ca 2+ entry through transient receptor potential (TRP) channels inhibited by 2‐APB (50 μM) and flufenamic acid (100 μM). Hydrophobic interactions between G q/11 ‐coupled P2Y purinoceptors and TRP channels were suggested by prevention of the ADP‐induced [Ca 2+ ] i plateau following PIP 2 depletion with LiCl (10 mM) and cholesterol removal from lipid rafts with methyl‐ß‐cyclodextrin (2 mM). ADP [Ca 2+ ] i transients were insensitive to P2Y 1 , P2Y 12 , and P2Y 13 receptor antagonists, MRS2179 (10μM), AR‐C66096 (0.1 μM), and MRS2211 (10μM), respectively, but were attenuated by suramin and reactive blue‐2 (100 μM) which also blocked P2Y 4 receptors activation by UTP. Cardiac myofibroblasts growth and type I collagen production were favored upon activation of MRS2179‐sensitive P2Y 1 receptors with ADP or ADPßS (30 μM). In conclusion, ADP exerts a dual role on ventricular myofibroblasts: [Ca 2+ ] i transients are mediated by fast‐desensitizing P2Y 4 receptors, whereas the pro‐fibrotic effect of ADP involves the P2Y 1 receptor activation. Data also show that ADP‐induced capacitative Ca 2+ influx depends on phospholipase C‐linked TRP channels opening in lipid raft microdomains. J. Cell. Physiol. 232: 1511–1526, 2017. © 2016 Wiley Periodicals, Inc.

Certal, Mariana↗

The Effect of Persistent Heavy Metal Exposure on Some Antioxidant Enzyme Activities and Lipid Peroxidation of the Freshwater snail, Lymnaea natalensis

This study investigated the effect of persistent heavy metal exposure on the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) of the freshwater snail, Lymnaea natalensis. Malondialdehyde (MDA) levels were also measured as an index of lipid peroxidation. The snails were exposed to cadmium, copper, lead and mercury for a total of 28 days at 0.1 mg/L, 0.1 mg/L, 0.2 mg/L and 0.1 mg/L respectively. Samples were collected at 1, 7, 14, 21 and 28 days intervals. Analysis of SOD showed significant initial increases in enzyme activity following exposure to copper, lead and mercury, while cadmium exposures caused increases from Day 14 onwards. Copper, cadmium and lead caused significant initial increases in CAT activity and mercury caused an increase only on Day 28. Copper caused a significant increase in GPx activity on Day 28 while MDA levels diminished significantly at Days 7–28. Similarly, cadmium caused significant increases of GPx activity on Day 28 whereas MDA levels were significantly reduced. Lead also caused a significant increase in GPx activity on Days 14–28 whilst no significant changes occurred in MDA levels. Mercury exposures caused significant increases in GPx activity on Days 7 and 21, whilst MDA levels were significantly reduced on Days 7 and 14. These findings suggest that persistent exposure of snails to heavy metals induces the antioxidant defence system, and decreases lipid peroxidation.

54 ENVIRONMENTAL SCIENCES↗

Förster Resonance Energy Transfer Study of Cytochrome c—Lipid Interactions

Specific interactions between a mitochondrial hemoprotein cytochrome c (cyt c) and cardiolipin, a lipid component of mitochondrial membrane, are crucial to electron shuttling and apoptotic activities of this protein. In the present study the Förster resonance energy transfer (FRET) between anthrylvinyl-labeled phosphatidylcholine as a donor and heme moiety of cyt c as an acceptor was employed to give a quantitative characterization of the protein binding to the model membranes from the mixtures of phosphatidylcholine (PC) with phosphatidylglycerol (PG), phosphatidylserine (PS) or cardiolipin (CL) in different molar ratios. The multiple arrays of the FRET data were globally analyzed in terms of the model of energy transfer in two-dimensional systems combined with the scaled particle adsorption model. The arguments in favor of the specificity of cyt c interactions with CL were obtained, including the higher adsorption potential and the deeper protein insertion in the lipid bilayer.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Emerging Role of Coronary Computed Tomography Angiography in Lipid-Lowering Therapy: a Bridge to Image-Guided Personalized Medicine

Purpose of Review: To summarize the current status of coronary computed tomography angiography (CTA) in the assessment of coronary plaques and discuss the ability of serial coronary CTA to quantitatively measure changes in the plaque burden in response to lipid-lowering therapy. Recent Findings: Recent advances in coronary CTA have allowed identification of high-risk coronary features in acute coronary syndrome and measurement of changes in the coronary plaque burden with good reproducibility. Statin therapy may delay plaque progression and change some plaque features. However, the clinical relevance of quantitative changes in coronary plaques and the optimal methods to reduce the plaque burden remain unclear. Summary: Despite guideline-directed lipid-lowering therapy, adverse events still occur in substantial numbers of patients receiving statins. Coronary CTA is noninvasive and has high diagnostic performance in patients with coronary artery disease, making change in the plaque burden an applicable biomarker for individualized assessment of future risk.

62 RADIOLOGY AND NUCLEAR MEDICINE↗

Pyrolysis Oil-Based Lipid Production as Biodiesel Feedstock by Rhodococcus opacus

Light oil from pyrolysis, which accounts for ~10 % carbon yield of the starting biomass, is a complex aqueous product that is difficult to utilize and usually discarded. This work presents the feasibility of light oil as a sole carbon source to support the growth of Rhodococcus opacus (R. opacus) that in turn accumulate triacylglycerols as biodiesel feedstock. Two types of bacteria (R. opacus PD630 and DSM 1069) were selected in this study. Research results showed that after short adaption periods both strains can grow well on this complex carbon source, as proved by the consumption of oligomers and monomers in light oil. Lipid content by R. opacus PD630 and DSM 1069 was observed up to 25.8 % and 22.0 % of cell dry weight, respectively. Palmitic and stearic acids were found to be the predominant fatty acids in these bacterial cells. In addition, the light oil-based lipid production can be enhanced by reducing the pH value from 7 to 4, especially in case of DSM 1069.

09 BIOMASS FUELS↗

Lipid Production by Arctic Microalga Chlamydomonas sp. KNF0008 at Low Temperatures

A lipid-producing microalga, Chlamydomonas sp. KNF0008, collected from the Arctic was capable of growing at temperatures ranging from 4 to 20 °C, and the highest cell density was measured at 15 °C and 100 μmol photons m{sup −2} s{sup −1} light intensity under continuous shaking and external aeration. KNF0008 showed the elevated accumulation of lipid bodies under nitrogen-deficient conditions, rather than under nitrogen-sufficient conditions. Fatty acid production of KNF0008 was 4.2-fold (104 mg L{sup −1}) higher than that of C. reinhardtii CC-125 at 15 °C in Bold’s Basal Medium. The dominant fatty acids were C16:0, C16:4, C18:1, and C18:3, and unsaturated fatty acids (65.69%) were higher than saturated fatty acids (13.65%) at 15 °C. These results suggested that Arctic Chlamydomonas sp. KNF0008 could possibly be utilized for production of biodiesel during periods of cold weather because of its psychrophilic characteristics.

60 APPLIED LIFE SCIENCES↗

Growth, total lipid, and omega-3 fatty acid production by Nannochloropsis spp. cultivated with raw plant substrate

Improving productivity and lipid concentration in microalgae is important for the economic success of both biofuel and microalgae coproducts production. Nannochloropsis spp. are marine microalgae currently being grown at large-scale for the production of biofuel and lipid coproducts. In this work, we demonstrate improvements of growth and omega-3 production in Nannochloropsis gaditana CCMP526 and Nannochloropsis oceanica CCAP849/10 with plant substrate addition, a potentially economical option for increasing microalgal productivity. Furthermore, we examine growth in the presence of corn stover, switchgrass, sugarcane bagasse, and yard waste. By examining the microbial ecology of N. gaditana cultures with and without plant substrate and with and without antibiotics, we discovered a potential bacterial interaction in these cultures, but its presence is not necessary for algal growth improvements in the presence of plant substrate. Analysis of plant substrate morphology by scanning electron microscopy (SEM) after cultivation in media with and without N. gaditana shows a degradation of specific plant structural features and a colonization of the plant phloem by N. gaditana. An examination of N. gaditana in a Congo red plate assay indicates potential cellulolytic activity, but a preliminary examination of the potential cellulase transcripts does not reveal differential expression of a candidate in the presence of plant. This evidence demonstrates potential raw plant utilization by a marine microalga for increased productivity and provides a potentially economical option for increasing coproduct concentration at industrial scale without genetic engineering.

59 BASIC BIOLOGICAL SCIENCES↗

Farnesoid X receptor up-regulates expression of Lipid transfer inhibitor protein in liver cells and mice

Highlights: •FXR up-regulates apoF. •It binds to ER1 element. •It activates apoF gene promoter. -- Abstract: Apolipoprotein F is a component protein mainly secreted by liver and resides on several lipoprotein classes. It can inhibit lipids transfer between different lipoproteins. FXR is a member of the nuclear receptor superfamily which is also highly expressed in the liver. It modulates bile acids synthesis and lipids metabolism by transcriptional regulation. We aimed to determine whether apoF can be regulated by FXR. The FXR agonist Chenodeoxycholic acid (CDCA) and GW4064 both can activate the expression of apoF in liver cell lines and in C57/BL6 mouse liver. This is dependent on the binding of FXR to the FXR element ER1 (−2904 to −2892 bp) in the apoF gene promoter. Taken together, we have identified apoF as likely another target gene of FXR.

60 APPLIED LIFE SCIENCES↗

Tamoxifen affects glucose and lipid metabolism parameters, causes browning of subcutaneous adipose tissue and transient body composition changes in C57BL/6NTac mice

Tamoxifen is a selective estrogen receptor (ER) modulator which is widely used to generate inducible conditional transgenic mouse models. Activation of ER signaling plays an important role in the regulation of adipose tissue (AT) metabolism. We therefore tested the hypothesis that tamoxifen administration causes changes in AT biology in vivo. 12 weeks old male C57BL/6NTac mice were treated with either tamoxifen (n = 18) or vehicle (n = 18) for 5 consecutive days. Tamoxifen treatment effects on body composition, energy homeostasis, parameters of AT biology, glucose and lipid metabolism were investigated up to an age of 18 weeks. We found that tamoxifen treatment causes: I) significantly increased HbA{sub 1c}, triglyceride and free fatty acid serum concentrations (p < 0.01), II) browning of subcutaneous AT and increased UCP-1 expression, III) increased AT proliferation marker Ki67 mRNA expression, IV) changes in adipocyte size distribution, and V) transient body composition changes. Tamoxifen may induce changes in body composition, whole body glucose and lipid metabolism and has significant effects on AT biology, which need to be considered when using Tamoxifen as a tool to induce conditional transgenic mouse models. Our data further suggest that tamoxifen-treated wildtype mice should be characterized in parallel to experimental transgenic models to control for tamoxifen administration effects. - Highlights: • Tamoxifen treatment causes significantly increased HbA{sub 1c}, triglyceride and free fatty acid serum concentrations. • Tamoxifen induces browning of subcutaneous AT and increased UCP-1 expression. • Tamoxifen changes adipocyte size distribution, and transient body composition.

60 APPLIED LIFE SCIENCES↗

FADS2 inhibition in essential fatty acid deficiency induces hepatic lipid accumulation via impairment of very low-density lipoprotein (VLDL) secretion

Highlights: • Mead acid (20:3n-9) is produced from oleic acid (18:1n-9) during essential fatty acid deficiency (EFAD). • Hepatic lipid accumulation was observed in EFAD mice treated with FADS2 inhibitor. • FADS2 inhibition during EFAD reduced VLDL secretion. • Mead acid in phosphatidylcholine was dramatically reduced by FADS2 inhibitor. • Mead acid may play the role of a substitute of C20 highly unsaturated fatty acids in VLDL secretion during EFAD. Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD). In this study, an obvious hepatic lipid accumulation was observed in EFAD mice treated with a FADS2 inhibitor. FADS2 inhibition in the EFAD state reduced secretion of very low-density lipoprotein (VLDL) and markedly diminished Mead acid in phosphatidylcholine (PC) in the liver and plasma. As the results, the amount of C20 HUFAs in hepatic and plasma PC dramatically reduced in the EFAD mice treated with a FADS2 inhibitor, whereas the decrease of C20 HUFA levels of PC in EFAD mice was not observed because of the increased Mead acid in PC. These results supposed that Mead acid in PC is important as a component of VLDL. It is possible that Mead acid plays the role of a substitute of HUFAs in VLDL secretion during EFAD.

60 APPLIED LIFE SCIENCES↗

An experimental study of ovarian cancer imaging and therapy by paclitaxel-loaded phase-transformation lipid nanoparticles combined with low-intensity focused ultrasound

Highlights: • PTX-anti-LHRHR-PTNPs is expected to become a new type of ultrasound contrast agent. • LIFU can enhance the efficiency of PTX-anti-LHRHR-PTNPs. • For ovarian cancer, PTX-anti-LHRHR-PTNPs has a good therapeutic effect. Drug-loaded phase-transformation lipid nanoparticles (NPs) combined with low-intensity focused ultrasound (LIFU) for ultrasound molecular imaging and therapy, which is a very promising drug carrier and can provide both physical and chemical therapeutics, simultaneously. We successfully prepared the paclitaxel (PTX) loaded anti-LHRHR targeted phase-transformation lipid nanoparticles (PTX-anti-LHRHR-PTNPs) for ovarian cancer in this study combined with LIFU has the following characteristics: On the one hand, it showed smaller size and greater stability than blood cells, which significantly prolonged its half-life in the body, and can actively target ovarian cancer OVCAR-3 cells, and smoothly penetrate the endothelial gap into the tumor site for specifically killing the ovarian cancer cells. Thereby, the special drug carrier improved the therapeutic effect and reduced toxic and side effects, maximized the protection of normal tissues and minimized adverse reactions. On the other hand, PTX-anti-LHRHR-PTNPs can be targeted to focus after being injected intravenously and remain in the tumor target tissue for a long time. At the same time, liquid-gas phase-transformation can occur under LIFU triggering, resulting in more ideal and sustained ultrasound imaging effects. Then acoustic contrast agent is used to develop the molecular level of ultrasound scattering, so as to evaluate the diseased tissue from the molecular level.

60 APPLIED LIFE SCIENCES↗