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At least 667 records · Page 37

Integrated active and passive control design methodology for the LaRC CSI evolutionary model

A general design methodology to integrate active control with passive damping was demonstrated on the NASA LaRC CSI Evolutionary Model (CEM), a ground testbed for future large, flexible spacecraft. Vibration suppression controllers designed for Line-of Sight (LOS) minimization were successfully implemented on the CEM. A frequency-shaped H2 methodology was developed, allowing the designer to specify the roll-off of the MIMO compensator. A closed loop bandwidth of 4 Hz, including the six rigid body modes and the first three dominant elastic modes of the CEM was achieved. Good agreement was demonstrated between experimental data and analytical predictions for the closed loop frequency response and random tests. Using the Modal Strain Energy (MSE) method, a passive damping treatment consisting of 60 viscoelastically damped struts was designed, fabricated and implemented on the CEM. Damping levels for the targeted modes were more than an order of magnitude larger than for the undamped structure. Using measured loss and stiffness data for the individual damped struts, analytical predictions of the damping levels were very close to the experimental values in the (1-10) Hz frequency range where the open loop model matched the experimental data. An integrated active/passive controller was successfully implemented on the CEM and was evaluated against an active-only controller. A two-fold increase in the effective control bandwidth and further reductions of 30 percent to 50 percent in the LOS RMS outputs were achieved compared to an active-only controller. Superior performance was also obtained compared to a High-Authority/Low-Authority (HAC/LAC) controller.

Voth, Christopher T.↗

A Comparative Study on Representativeness and Stochastic Efficacy of Miniature Tensile Specimen Testing

Here, in this article, a miniature dog bone tensile coupon design was tested against the existing ASTM standard specimen design. Specimens were prepared from commercially sourced austenitic stainless steel 304 alloy, and a defect-ridden additively manufactured 304L alloy was studied. By utilizing a tensile specimen design that is 1/230th volume of the smallest ASTM E8-04(2016), Standard Test Methods for Tension Testing of Metallic Materials, dog bone specimen, coupled to a digital image correlation (DIC) setup, case studies were performed to compare tensile property measurements and strain field evolution. Whereas yield strength measurements were observed to be similar, post-yield, the ultimate strength measurements and ductility measurements from the miniature specimens were observed to be higher than the ASTM specimen design. Although the strength measurements were comparable, the strain evolution was found to differ in the miniature specimens. Studies to assess effects of varying thickness and defect population were also pursued on the miniature tensile specimen. From the DIC strain field estimations, the peak local strain values at ultimate tensile strength were observed to be increasing with reducing specimen thickness. Testing of defect ridden stainless steel revealed the sensitivity to failure through strain localization and the influence of defect size was captured in the strength measurements.

36 MATERIALS SCIENCE↗

Characterization of a Mo-Nitrogenase Variant Containing a Citrate-Substituted Cofactor

Nitrogenase converts N 2 to NH 3 , and CO to hydrocarbons, at its cofactor site. In this work, we report a biochemical and spectroscopic characterization of a Mo-nitrogenase variant expressed in an Azotobacter vinelandii strain containing a deletion of nifV, the gene encoding the homocitrate synthase. Designated NifDK Cit , the catalytic component of this Mo-nitrogenase variant contains a citrate-substituted cofactor analogue. Activity analysis of NifDK Cit reveals a shift of CO reduction from H 2 evolution toward hydrocarbon formation and an opposite shift of N 2 reduction from NH 3 formation toward H 2 evolution. Consistent with a shift in the Mo K-edge energy of NifDK Cit relative to that of its wild-type counterpart, EPR analysis demonstrates a broadening of the line-shape and a decrease in the intensity of the cofactor-originated S=3/2 signal, suggesting a change in the spin properties of the cofactor upon citrate substitution. These observations point to a crucial role of homocitrate in substrate reduction by nitrogenase and the possibility to tune product profiles of nitrogenase reactions via organic ligand substitution.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Evaluation of the recombinant proteins RlpB and VacJ as a vaccine for protection against Glaesserella parasuis in pigs

Abstract Background Glaesserella parasuis , the causative agent of Glӓsser’s disease, is widespread in swine globally resulting in significant economic losses to the swine industry. Prevention of Glӓsser’s disease in pigs has been plagued with an inability to design broadly protective vaccines, as many bacterin based platforms generate serovar or strain specific immunity. Subunit vaccines are of interest to provide protective immunity to multiple strains of G. parasuis . Selected proteins for subunit vaccination should be widespread, highly conserved, and surface exposed. Results Two candidate proteins for subunit vaccination (RlpB and VacJ) against G. parasuis were identified using random mutagenesis and an in vitro organ culture system. Pigs were vaccinated with recombinant RlpB and VacJ, outer membrane proteins with important contributions to cellular function and viability. Though high antibody titers to the recombinant proteins and increased interferon-γ producing cells were found in subunit vaccinated animals, the pigs were not protected from developing systemic disease. Conclusions It appears there may be insufficient RlpB and VacJ exposed on the bacterial surface for antibody to bind, preventing high RlpB and VacJ specific antibody titers from protecting animals from G. parasuis . Additionally, this work confirms the importance of utilizing the natural host species when assessing the efficacy of vaccine candidates.

Hau, Samantha J.↗

Isolation and characterization of IgG3 glycan-targeting antibodies with exceptional cross-reactivity for diverse viral families

Broadly reactive antibodies that target sequence-diverse antigens are of interest for vaccine design and monoclonal antibody therapeutic development because they can protect against multiple strains of a virus and provide a barrier to evolution of escape mutants. Using LIBRA-seq (linking B cell receptor to antigen specificity through sequencing) data for the B cell repertoire of an individual chronically infected with human immunodeficiency virus type 1 (HIV-1), we identified a lineage of IgG3 antibodies predicted to bind to HIV-1 Envelope (Env) and influenza A Hemagglutinin (HA). Two lineage members, antibodies 2526 and 546, were confirmed to bind to a large panel of diverse antigens, including several strains of HIV-1 Env, influenza HA, coronavirus (CoV) spike, hepatitis C virus (HCV) E protein, Nipah virus (NiV) F protein, and Langya virus (LayV) F protein. We found that both antibodies bind to complex glycans on the antigenic surfaces. Antibody 2526 targets the stem region of influenza HA and the N-terminal domain (NTD) region of SARS-CoV-2 spike. A crystal structure of 2526 Fab bound to mannose revealed the presence of a glycan-binding pocket on the light chain. Antibody 2526 cross-reacted with antigens from multiple pathogens and displayed no signs of autoreactivity. These features distinguish antibody 2526 from previously described glycan-reactive antibodies. Further study of this antibody class may aid in the selection and engineering of broadly reactive antibody therapeutics and can inform the development of effective vaccines with exceptional breadth of pathogen coverage.

Microbiology↗

Development of an accelerated creep testing technique via multi-specimen loading

This report summarizes a research effort undertaken by Argonne National Laboratory to accelerate the development of creep data package for code qualification. The goal is to explore new creep testing techniques to shorten the overall time needed to generate high-quality data to support an ASME code case on laser powder bed fusion 316H stainless steel. Progresses on the design and implementation of a 3-specimen load train and an innovative strain measurement method with direct potential current drop (DCPD) technique are reported.

22 GENERAL STUDIES OF NUCLEAR REACTORS↗

Enhancing photonic systems using topology and non-Hermiticity

The broad goal of this project was to develop new analytical and numerical insights for how important photonic processes can be improved using recently discovered principles in topological and non-Hermitian physics. In particular, there are two recent discoveries that we aimed to harness to achieve this goal. First, it was discovered in condensed matter physics that crystalline symmetries can protect low-dimensional topologically protected states in lattices without the need for breaking time-reversal symmetry. These so-called ‘higher-order’ topological systems represent an important development for photonic systems, where it is very difficult to break time-reversal symmetry, and which had been previously thought necessary to realize topological phenomena. Second, the last decade has seen a significant amount of interest in phenomena which are unique to non-Hermitian systems, i.e., systems which do not conserve energy. For example, spatially patterned gain and loss can be used to realize exceptional points, which are degeneracies in a system’s spectrum where the system becomes defective, while the existence of radiative losses also enables a new route to confinement through bound states in the continuum. For such non-Hermitian phenomena, photonics again represents a critical platform, as photonic systems naturally lose light to their radiative environments, making them generally non-Hermitian, and it is also possible to incorporate additional gain or loss. Based on these broad principles, we pursued a range of projects to harness bound states in the continuum in a variety of different systems and architectures, develop real-space methods for classifying topological systems to yield better photonic design principles, and a novel Brillouin-based fiber laser for sensing strain.

77 NANOSCIENCE AND NANOTECHNOLOGY↗

T3tris: AI-Driven Inverse Design of Cellular Materials

Los Alamos National Laboratory has developed T3tris, a generative AI platform that enables real-time inverse design of architected cellular materials. This capability transforms how mechanical metamaterials are designed by generating microstructural topologies that conform to target nonlinear stress-strain curves, even at high compressive strain (up to 50%).

36 MATERIALS SCIENCE↗

Study of the effect of the trancription factor SARO_RS14285 in aromatics degradation in Novosphingobium aromaticivorans

Novosphingobium aromaticivorans DSM12444 is a bacterium capable of catabolizing several lignin aromatics. A main degradation pathway for these compounds is the protocatechuate (PCA) meta-cleavage. Nevertheless, the transcriptional regulation of this pathway is still unknown. Immediately upstream of the genes encoding the enzymes for this pathway, the LysR-type transcription factor (LTTF) SARO_RS14285 was identified. To evaluate the functionality of this LTTF, a deletion mutant was constructed. A transcriptomic analysis of the mutant strain cultured in several aromatic compounds is included in this report. Overall design: RNA-seq profiling of the WT and the deletion mutant cultured in minimal medium with glucose and one of the following aromatics: protocatechuic acid (PCA), 4-coumaric acid (4-CA), vanillic acid (VA) or syringic acid (SA).

aromatics↗

Dryden Flight Research Center hot structures research

The facilities, testing techniques, and design methods are described for NASA Dryden Flight Research Center. High temperature strain gage technology, realistic flight hardware fabrication, and structural analysis are discussed. A considerable amount of experimental work on hot structure concepts for hypersonic vehicles was performed; all the work is not complete, and there are still problem areas that need to be resolved.

Fields, R. A.↗

Galileo spacecraft modal test and evaluation of testing techniques

The structural configuration, modal test requirements and pre-test activities involved in modeling the expected dynamic environment and responses of the Galileo spacecraft are discussed. The probe will be Shuttle-launched in 1986 and will gather data on the Jupiter system. Loads analysis for the 5300 lb spacecraft were performed with the NASTRAN code, and covered 10,000 static degrees of freedom and 1600 mass degrees of freedom. A modal analysis will be used to verify the predictions for natural frequencies, mode shapes, orthogonality checks, residual mass, modal damping and forces, and generalized forces. Verification of the validity of considering only 70 natural modes in the numerical simulation is being performed by examining the forcing functions of the analysis. The analysis led to requirements that 162 channels of accelerometer data and 118 channels of strain gage data be recorded during shaker tests to reveal areas where design changes will be needed to eliminate vibration peaks.

Chen, J.-C.↗

Life prediction and constitutive models for engine hot section anisotropic materials program

The purpose is to develop life prediction models for coated anisotropic materials used in gas temperature airfoils. Two single crystal alloys and two coatings are now being tested. These include PWA 1480; Alloy 185; overlay coating, PWA 286; and aluminide coating, PWA 273. Constitutive models are also being developed for these materials to predict the plastic and creep strain histories of the materials in the lab tests and for actual design conditions. This nonlinear material behavior is particularily important for high temperature gas turbine applications and is basic to any life prediction system.

Swanson, G. A.↗

Resistance To Delamination In Composite Materials

Fracture-mechanics approach applied to delamination failures. Report presents fracture-mechanics approach to analyzing, characterizing, and designing against delamination. In study, examples of delamination problems illustrated wherein strain-energy-release rate associated with growth of delamination useful generic parameter, independent of thickness, layup, and source of delamination, for characterization of failure by delamination. Several techniques for calculation of strain-energy-release rates for delaminations from variety of sources outlined. Technique for quantification of durability due to cyclic loading presented.

Obrien, T. Kevin↗

Life prediction and constitutive models for engine hot section

The purpose of this program is to develop life prediction models for coated anisotropic materials used in gas turbine airfoils. In the program, two single crystal alloys and two coatings are being tested. These include PWA 1480, Alloy 185, overlay coating (PWA 286), and aluminide coating (PWA 273). Constitutive models are also being developed for these materials to predict the time independent (plastic) and time dependent (creep) strain histories of the materials in the lab tests and for actual design conditions. This nonlinear material behavior is particularly important for high temperature gas turbine applications and is basic to any life prediction system. Some of the accomplishments of the program are highlighted.

Swanson, G. A.↗

Space Shuttle Main Engine structural analysis and data reduction/evaluation. Volume 1: Aft Skirt analysis

Using the ANSYS finite element program, a global model of the aft skirt and a detailed nonlinear model of the failure region was made. The analysis confirmed the area of failure in both STA-2B and STA-3 tests as the forging heat affected zone (HAZ) at the aft ring centerline. The highest hoop strain in the HAZ occurs in this area. However, the analysis does not predict failure as defined by ultimate elongation of the material equal to 3.5 percent total strain. The analysis correlates well with the strain gage data from both the Wyle influence test of the original design aft sjirt and the STA-3 test of the redesigned aft skirt. it is suggested that the sensitivity of the failure area material strength and stress/strain state to material properties and therefore to small manufacturing or processing variables is the most likely cause of failure below the expected material ultimate properties.

Berry, David M.↗

Development of a dual strain gage balance system for measuring light loads

A strain-gage-balance (SGB) force-measurement system is described which was designed to meet light-load requirements for an airfoil model tested in the NASA Langley low-turbulence pressure tunnel (LTPT). This system was developed to obtain direct force data needed to verify calculated pressure/force correlations used in previous aerodynamic tests. The three-component force-measurement system was designed so that the SGBs would simultaneously support and measure the following loads: weight of the airfoil (approximately 3.0 lbf); 8.0 lbf of lift; 0.5 lbf of drag; and 16.0 in. lbf of pitching moment. In addition to these design loads, the system was required to withstand 100-percent overload on all three components. The system comprises an airfoil, two SGBs, a thermal flexure, and a mounting plate. The installation of the system in the LTPT is also discussed.

Roberts, Paul W.↗

A variable stiffness strut using strain induced bending for vibration suppression in the evolutionary model

A new piezoceramic strut has been developed. This strut utilizes strain induced bending as a means of actuation. The significance of this design is that the displacement of the strut's motion arm is independent of the expansion of the piezoceramic material. Design criteria was developed using continuous and finite element modeling techniques. Experimental and analytical results were compared. Finally, a numerical analysis was conducted that demonstrated the benefits of the piezoceramic strut configuration.

Pokines, Brett↗

The Pack Method for Compressive Tests of Thin Specimens of Materials Used in Thin-Wall Structures

The strength of modern lightweight thin-wall structures is generally limited by the strength of the compression members. An adequate design of these members requires a knowledge of the compressive stress-strain graph of the thin-wall material. The "pack" method was developed at the National Bureau of Standards with the support of the National Advisory Committee for Aeronautics to make possible a determination of compressive stress-strain graphs for such material. In the pack test an odd number of specimens are assembled into a relatively stable pack, like a "pack of cards." Additional lateral stability is obtained from lateral supports between the external sheet faces of the pack and outside reactions. The tests seems adequate for many problems in structural research.

Aitchison, C S↗