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At least 667 records · Page 37

Transcriptomic Profiles of Zymomonas mobilis 8b to Furfural Acute and Long-Term Stress in Both Glucose and Xylose Conditions

Zymomonas mobilis 8b is an ethanologenic bacterium engineered to utilize both glucose and xylose. The impacts of lignocellulosic hydrolyzate inhibitors on the growth of Zymomonas mobilis 8b have been investigated. However, the molecular responses of these inhibitors have not been completely elucidated yet. In this study, molecular responses to furfural were investigated using transcriptomic approaches of both chip-based microarray and a directional mRNA-Seq. Furfural acute shock time-course experiment with 3 g/L furfural supplemented when cells reached exponential phase and stress response experiment in the presence of 2 g/L furfural from the beginning of fermentation were carried out to study the physiological and transcriptional profiles of short-term and long-term effects of furfural on 8b. Furfural negatively affected 8b growth in terms of final biomass and the fermentation time. Transcriptomic studies indicated that the response of 8b to furfural was dynamic and complex, and differences existed between short-term shock and long-term stress responses. However, the gene function categories were similar with most down-regulated genes related to translation and biosynthesis, while the furfural up-regulated genes were mostly related to general stress responses. Several gene candidates have been identified and genetic studies indicated that expression of ZMO0465 and cysteine synthase operon ZMO0003-0006 driven by its native promoter in a shuttle vector enhanced the furfural tolerance of 8b. In addition, the relationship between microarray and mRNA-Seq was compared with good correlations. The directional mRNA-Seq data not only provided the gene expression profiling, but also can be applied for transcriptional architecture improvement to identify and confirm operons, novel transcripts, hypothetical gene functions, transcriptional start sites, and promoters with different strength.

09 BIOMASS FUELS↗

A method for the modelling of porous and solid wind tunnel walls in computational fluid dynamics codes

Porous wall wind tunnels have been used for several decades and have proven effective in reducing wall interference effects in both low speed and transonic testing. They allow for testing through Mach 1, reduce blockage effects and reduce shock wave reflections in the test section. Their usefulness in developing computational fluid dynamics (CFD) codes has been limited, however, by the difficulties associated with modelling the effect of a porous wall in CFD codes. Previous approaches to modelling porous wall effects have depended either upon a simplified linear boundary condition, which has proven inadequate, or upon detailed measurements of the normal velocity near the wall, which require extensive wind tunnel time. The current work was initiated in an effort to find a simple, accurate method of modelling a porous wall boundary condition in CFD codes. The development of such a method would allow data from porous wall wind tunnels to be used more readily in validating CFD codes. This would be beneficial when transonic validations are desired, or when large models are used to achieve high Reynolds numbers in testing. A computational and experimental study was undertaken to investigate a new method of modelling solid and porous wall boundary conditions in CFD codes. The method utilized experimental measurements at the walls to develop a flow field solution based on the method of singularities. This flow field solution was then imposed as a pressure boundary condition in a CFD simulation of the internal flow field. The effectiveness of this method in describing the effect of porosity changes on the wall was investigated. Also, the effectiveness of this method when only sparse experimental measurements were available has been investigated. The current work demonstrated this approach for low speed flows and compared the results with experimental data obtained from a heavily instrumented variable porosity test section. The approach developed was simple, computationally inexpensive, and did not require extensive or intrusive measurements of the boundary conditions during the wind tunnel test. It may be applied to both solid and porous wall wind tunnel tests.

Beutner, Thomas John↗

Shock waves generated by the intense solar flare of 1972, August 7, 15:00 UT

The dynamic radio spectrum of the class 3B solar flare of 1972, August 7, 15:00 UT, over the band 10 to 2000 MHz is examined. Type II and type IV bursts in the spectrum are interpreted in terms of a piston-driven shock, which appeared to be traveling at a velocity of about 1500 km per sec and which generated pulsations in the band 100 to 200 MHz as it passed through the corona. The progress of the shock through the interplanetary plasma was subsequently monitored by Malitson et al. with radio equipment covering the band 0.03 to 2.6 MHz on the IMP-6 satellite.

Maxwell, A.↗

A simple model of a strong shock driven by a spherical or cylindrical piston

A simple model of piston-driven spherical and cylindrical shocks is suggested. The model is based on a consistent use of two factors: (a) an almost uniform pressure across the shocked layer and, (b) continuous geometrical stretching of the surface elements of the expanding piston. It turns out that for a uniform pre-shock medium the gas between the piston and the shock behaves essentially as an incompressible fluid. An algebraic equation for the shock vs piston position is obtained. Detailed evaluation of the accuracy of the proposed solution shows that its accuracy is a few percent for the adiabatic index γ = 5/3 (as in ideal plasma). A closed-form solution describing enhancement of a weak ambient magnetic field by the shock is presented. The proposed model of piston-driven shocks goes beyond the classical self-similar solutions in that it: (1) naturally covers an early, non-asymptotic dynamics and its transition to asymptotic regime; (2) allows for smooth radial density variation of an ambient gas of the form of bumps, dimples or ramps between two constant values; and (3) allows for smooth temporal variation of piston velocity of the form of bumps, dimples, or ramps. Furthermore, this simple and versatile model provides some new insights into a classical hydrodynamical problem.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

A self-consistent theory of photohydrodynamical shocks

A formalism is developed which simultaneously describes the evolution of both the photon distribution and the electron temperature in radiation-dominated shocks. The procedure permits the imposition of an arbitrary spectrum at any chosen optical depth and provides results which automatically satisfy the dynamical constraints derived by Blandford and Payne (1981). It is noted that radiation-dominated shocks may play an important role in a variety of astrophysical environments, from accreting neutron stars to quasars and the early universe.

Becker, Peter A.↗

Establishing temperature from radiance of dynamically compressed metals

Precise temperature determination is a significant challenge in extreme environments of dynamic compression studies. How can radiance measurements taken in high-pressure shock experiments constrain temperature in a meaningful and physically consistent way? Experiments maintaining sample compression against a transparent window can be tailored to present a uniform measurement area with uncertain spectral emissivity. We compare several methods to analyze radiance collected at multiple wavelengths, applying statistical methods and physical principles to improve temperature inference. With proper radiance collection and analysis, dynamic temperature uncertainties become comparable to thermomechanical ambiguities of the emitting surface.

36 MATERIALS SCIENCE↗

Dynamic properties of mare basalts - Relation of equation of state to petrology

A comparison of shock compression and release adiabat data to 160 GPa for the 12063 low-titanium mare basalt with previous results for the high-titanium, 70215 mare basalt shows that, while the two rocks have very similar zero-pressure densities, the former is much less compressible above 100 GPa. This demonstrates that rocks with complex mineralogies can have significantly different dynamic properties despite a similarity of oxide components. The 10% higher ilmenite content of 70215 is suggested as an explanation for the contrasting dynamic properties of the two rocks. Because the 12063 high-pressure data presented lie midway between the 70215 data and those previously obtained for the anorthosite that is the dominant constituent of the lunar highlands, it is concluded that the behavior of lunar surface material under dynamic compression is terrane-dependent; where this dependence is (1) found to extend to different regions of the maria, and (2) may affect the determination of the relative ages of different rock units from areal crater densities.

Ahrens, T. J.↗

A field programmable gate array-based timing and control system for the dynamic compression sector

A field programmable gate array (FPGA)-based timing and trigger control system has been developed for the Dynamic Compression Sector (DCS) user facility located at the Advanced Photon Source (APS) at Argonne National Laboratory. The DCS is a first-of-its-kind capability dedicated to dynamic compression science. All components of the DCS laser shock station—x-ray choppers, single-shot shutter, internal laser triggers, and shot diagnostics—must be synchronized with respect to the arrival of x rays in the hutch. An FPGA synchronized to the APS storage ring radio frequency clock (352 MHz) generates trigger signals for each stage of the laser and x-ray shutter system with low jitter. The developed FPGA-based control system was the first system used to control the laser and the shutter system since its commissioning, and it has been developing since then to improve the timing jitter. The system is composed of a Zynq FPGA, a debug card, line drivers, and a power supply. The delay and offsets of the trigger signals can be adjusted by using a user-friendly graphical user interface with high precision. Here, the details of the system architecture, timing requirements, firmware, and software implementation along with the performance evaluation are presented in this paper. The system offers low timing jitter (15.5 ps rms) with respect to the APS 352 MHz clock, suitable for the 100 ps (FWHM) x-ray bunch duration at the APS.

47 OTHER INSTRUMENTATION↗

CO Laser Absorption Measurements During Syngas Combustion at High Pressure

Abstract Syngas is a desirable fuel for combustion in the Allam-Fetvedt cycle, which involves combustion under supercritical-CO2 conditions. While some work has been conducted in collecting ignition delay times (IDT) at the extreme pressures required by these systems, significant model deficiencies remain. Additionally, considerable barriers in terms of nonideal gas dynamic effects have been shown for these experiments in shock tubes. Further investigation into the fundamental combustion kinetics of H2/CO/CO2 mixtures is required. Time-resolved speciation measurements for target species have been shown to better aid in improving the understanding of underlying chemical kinetics than global ignition delay time measurements. Therefore, laser absorption measurements of CO were measured behind reflected shock waves during combustion of syngas at 5 and 10 bar and temperatures between 1080 and 2100 K. The mixtures investigated utilized H2-to-CO ratios of 1:1 and 1:4, respectively, each at stoichiometric conditions, allowing for discussions of the effect of initial fuel composition. A ratio of fuel to CO2 of 1:2 was also utilized to represent commercially available syngas. The mixtures were diluted in helium and argon (20% He, 76.5% Ar) to minimize thermal effects and to expedite CO thermal relaxation during the experiment. The resulting CO time histories were then compared to modern chemical kinetics mechanisms, and disagreement is seen for this system, which is assumed to be fairly well known. This study elucidates particular chemistry that needs improvement in moving toward a better understanding of syngas combustion at elevated pressures.

Engineering↗

Review of delta wing space shuttle vehicle dynamics

The unsteady aerodynamics of the proposed delta planform, high cross range, shuttle orbiters, are investigated. It is found that these vehicles are subject to five unsteady-flow phenomena that could compromise the flight dynamics. The phenomena are as follows: (1) leeside shock-induced separation, (2) sudden leading-edge stall, (3) vortex burst, (4)bow shock-flap shock interaction, and (5) forebody vorticity. Trajectory shaping is seen as the most powerful means of avoiding deterimental effects of the stall phenomena; however, stall must be fixed or controlled when traversing the stall region. Other phenomana may be controlled by carefully programmed control deflections and some configuration modifications. Ways to alter the occurrence of the various flow conditions are explored.

Reding, J. P.↗

Study of vibrations produced by a vibrating beam used for vibrating concretes

For compacting concretes in building, vibrating beams are used. The vibrations are generated by inertial vibrators, and the beam is normally displaced by the operator by means of a handle that is elastically fastened to the beam by means of rubber pads. Considered are vibrations transmitted to the operator, taking into account the beam's shock vibration motions. The steady state motion of a dynamic beam pattern is studied, and results of experimental tests with existing equipment are presented.

Silas, C.↗

An experimental study of massive blowing from a nosetip during Jovian entry

The fluid dynamic structure and stability of the boundary layer and shock layer ahead of a highly blowing heat shield in an environment simulating entry into the Jovian atmosphere were investigated. The experimental studies were conducted in the Calspan 96 in shock tunnel with a freestream of a H2/He mixture at Mach numbers above 10. Measurements of heat transfer and pressure were made on the porous model surface, and high-speed schlieren photography was used to examine the structure and stability of the shock layer. Results are presented which show how the heat transfer and shock layer stability vary for gases of different molecular weights for a range of Reynolds numbers and mass-addition rates.

Holden, M. S.↗

Investigation of transonic region of high dynamic response encountered on an elastic supercritical wing

Unsteady aerodynamic data were measured on an aspect ratio 10.3 elastic supercritical wing while undergoing high dynamic response above a Mach number of 0.90. These tests were conducted in the NASA Langley Transonic Dynamics Tunnel. A previous test of this wing predicted an unusual instability boundary based on subcritical response data. During the present test no instability was found, but an angle of attack dependent narrow Mach number region of high dynamic wing response was observed over a wide range of dynamic pressures. The effect on dynamic wing response of wing angle of attack, static outbound control surface deflection and a lower surface spanwise fence located near the 60 percent local chordline was investigated. The driving mechanism of the dynamic wing response appears to be related to chordwise shock movement in conjunction with flow separation and reattachment on both the upper and lower surfaces.

Seidel, David A.↗

Investigation of transonic region of high dynamic response encountered on an elastic supercritical wing

Unsteady aerodynamic data were measured on an aspect ratio 10.3 elastic supercritical wing while undergoing high dynamic response above Mach number of 0.90. These tests were conducted in the NASA Langley Transonic Dynamics Tunnel. A previous test of this wing predicted an unusual instability boundary based upon subcritical response data. During the present test no instability was found, but an angle of attack dependent narrow Mach number region of high dynamic wing response was observed over a wide range of dynamic pressures. The effect on dynamic wing response of wing angle of attack, static outboard control surface deflection and a lower surface spanwise fence located near the 60 percent local chordline was investigated. The driving mechanism of the dynamic wing response appears to be related to chordwise shock movement in conjunction with flow separation and reattachment on both the upper and lower surfaces.

Seidel, David A.↗

Hypersonic research at Stanford University

The status of the hypersonic research program at Stanford University is discussed and recent results are highlighted. The main areas of interest in the program are the numerical simulation of radiating, reacting and thermally excited flows, the investigation and numerical solution of hypersonic shock wave physics, the extension of the continuum fluid dynamic equations to the transition regime between continuum and free-molecule flow, and the development of novel numerical algorithms for efficient particulate simulations of flowfields.

Candler, Graham↗

Tracking flow features using overset grids

A method is proposed to use overset grid topology to track dynamic flow features. Features of interest such as moving shock waves and vortices are overset with relatively fine tracker grids. Solutions are computed on the various grids and information is exchanged at intergrid boundaries. A grid track-sensor variable such as pressure is used to track the position of the flow feature to be resolved. The tracker grid is moved to the position where the track-sensor variable has the desired value (generally a maximum or a minimum) and new interpolation coefficients are computed for information exchange across grid boundaries. Solutions are computed at the current location and time-step, and grid motion is brought into the solution via time metrics. The method is demonstrated by tracking a moving shock and vortices shed behind a circular cylinder. It is conjectured that the method would show significant benefits in resolving features such as wakes behind oscillating airfoils and trajectories of jets issuing from rotating nozzles as encountered during thrust-vectoring.

Chawla, Kalpana↗