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NASA NTRS2021

Real-Time Culture-Independent Microbial Profiling Onboard the International Space Station using Nanopore Sequencing

For the past two decades, microbial monitoring of the International Space Station (ISS) has relied on culture-dependent methods that require return to Earth for analysis. This has a number of limitations, with the most significant being bias towards the detection of culturable organisms and the inherent delay between sample collection and ground-based analysis. In recent years, portable and easy-to-use molecular-based tools, such as Oxford Nanopore Technologies’ MinION™ sequencer and miniPCR bio’s miniPCR™ thermal cycler, have been validated onboard the ISS. Here, we report on the development, validation, and implementation of a swab-to-sequencer method that provides a culture-independent solution to real-time microbial profiling onboard the ISS. Method development focused on analysis of swabs collected in a low-biomass environment with limited facility resources and stringent controls on allowed processes and reagents. ISS-optimized procedures included enzymatic DNA extraction from a swab tip, bead-based purifications, altered buffers, and the use of miniPCR and the MinION. Validation was conducted through extensive ground-based assessments comparing current standard culture-dependent and newly developed culture-independent methods. Similar microbial distributions were observed between the two methods; however, as expected, the culture-independent data revealed microbial profiles with greater diversity. Protocol optimization and verification was established during NASA Extreme Environment Mission Operations (NEEMO) analog missions 21 and 22, respectively. Unique microbial profiles obtained from analog testing validated the swab-to-sequencer method in an extreme environment. Finally, four independent swab-to-sequencer experiments were conducted onboard the ISS by two crewmembers. Microorganisms identified from ISS swabs were consistent with historical culture-based data, and primarily consisted of commonly observed human-associated microbes. This simplified method has been streamlined for high ease-of-use for a non-trained crew to complete in an extreme environment, thereby enabling environmental and human health diagnostics in real-time as future missions take us beyond low-Earth orbit.

microbiology↗

NASA NTRSDate not supplied

Predicting Fiber Failure of Plain Weave Fabric with Recursive Multiscale Micromechanics

Recent advances in the development of machine learning (ML) algorithms have enabled the creation of predictive models that can improve decision making, decrease computational cost, and improve efficiency in a variety of fields. As an organization begins to develop and implement such models, the data used in the training, validation, and testing of machine learning models, the model parameters, and the use cases or limitations of the models must be properly stored to ensure models are both fully traceable and used correctly. In the context of predicting material behavior, advances in computationally intense, physics-based, modeling of material behavior at various length scales, and the emergence of Integrated Computational Materials Engineering (ICME) have driven the need for developing data-driven surrogate models of the physics-based simulation tools using machine learning (ML) techniques. Surrogate model development allows for accurate material behavior prediction at a fraction of the cost of its physics-based counterpart, allowing for multiscale simulations of real-world applications, further enabling the ability to design fit-for-purpose materials for a reasonable computational investment. However, training such models requires extensive data, and thus effective data management is necessary to reach the full potential that ML can offer to material design and ICME. This paper proposes a generalized, robust schema that allows organizations to store both real (experimental) and virtual (simulation) data used to train machine learning models and the defining model parameters and architectures. The developed schema allows for various types of data inputs and outputs, including single point values, time-series data, and images that can be used in for various types of machine learning models while following outlined best practices for effective data management. An effective schema for machine learning data and models can help prevent the recreation of virtual/real training data and surrogate models, can help reduce the time to create new models similar to existing ones by offering a starting point in the hyperparameter determination stages, minimize resources devoted to verification and validation (V&V) and certification of models, and ensure that data and surrogate models are not misused due to full traceability of both the data and ML model. It also allows organizations access to models that have already been developed, such that they can be used in the design of new materials, enabling the overall goals of ICME.

Failure↗

NASA NTRS1984

Nimbus-Earth radiation budget instrument analysis

The Wide Field Of View (WFOV) Earth Flux Channels of the Nimbus Earth Radiation Budget (ERB) instrument are studied with the objective of improving the understanding and confidence in the data. Laboratory tests on flight space Earth flux subassemblies followed by a set of in flight verification procedures are examined to see if the laboratory derived correction factors produced consistent results. Intercomparisons between the WFOV data and integrated scanner data as well as other truth are used in these verificaton procedures. The main source of errors are temperature and temperature gradient related offsets. The findings led to construction of a model for the short wave WFOV channel which use instrument temperatures and temperature differences to predict or establish offsets which need to be applied to data reduction algorithms for maximum final data product accuracy.

Maschhoff, R. M.↗

NASA NTRS1988

Astronomical data analysis from remote sites

The development of telescience methodologies for teledesign, teleoperation, and teleanalysis, and the possible verification of these concepts using the Extreme UV Explorer (EUVE) are discussed. The EUVE is a satellite payload scheduled for launch in 1991. The use of the Browse data base system (Star et al., 1987) is reviewed and its suitability for astronomy applications is evaluated. The possibility of adopting the Browse system for EUVE data analysis is considered.

Chakrabarti, Supriya↗

NASA NTRS2008

Guidance and Control Software Project Data - Volume 1: Planning Documents

The Guidance and Control Software (GCS) project was the last in a series of software reliability studies conducted at Langley Research Center between 1977 and 1994. The technical results of the GCS project were recorded after the experiment was completed. Some of the support documentation produced as part of the experiment, however, is serving an unexpected role far beyond its original project context. Some of the software used as part of the GCS project was developed to conform to the RTCA/DO-178B software standard, "Software Considerations in Airborne Systems and Equipment Certification," used in the civil aviation industry. That standard requires extensive documentation throughout the software development life cycle, including plans, software requirements, design and source code, verification cases and results, and configuration management and quality control data. The project documentation that includes this information is open for public scrutiny without the legal or safety implications associated with comparable data from an avionics manufacturer. This public availability has afforded an opportunity to use the GCS project documents for DO-178B training. This report provides a brief overview of the GCS project, describes the 4-volume set of documents and the role they are playing in training, and includes the planning documents from the GCS project. Volume 1 contains five appendices: A. Plan for Software Aspects of Certification for the Guidance and Control Software Project; B. Software Development Standards for the Guidance and Control Software Project; C. Software Verification Plan for the Guidance and Control Software Project; D. Software Configuration Management Plan for the Guidance and Control Software Project; and E. Software Quality Assurance Activities.

Hayhurst, Kelly J.↗

NASA NTRS2011

Adapting CALIPSO Climate Measurements for Near Real Time Analyses and Forecasting

The Cloud-Aerosol Lidar and Infrared Pathfinder satellite Observations (CALIPSO) mission was originally conceived and designed as a climate measurements mission, with considerable latency between data acquisition and the release of the level 1 and level 2 data products. However, the unique nature of the CALIPSO lidar backscatter profiles quickly led to the qualitative use of CALIPSO?s near real time (i.e., ? expedited?) lidar data imagery in several different forecasting applications. To enable quantitative use of their near real time analyses, the CALIPSO project recently expanded their expedited data catalog to include all of the standard level 1 and level 2 lidar data products. Also included is a new cloud cleared level 1.5 profile product developed for use by operational forecast centers for verification of aerosol predictions. This paper describes the architecture and content of the CALIPSO expedited data products. The fidelity and accuracy of the expedited products are assessed via comparisons to the standard CALIPSO data products.

Vaughan, Mark A.↗

NASA NTRS1994

ASF Design Considerations for Radarsat/ERS-2

This paper examines the requirements and the design considerations of the Synthetic Aperture Radar (SAR) ground data system for the Alaska SAR Facility (ASF) at the University of Alaska in Fairbanks (UAF) for the new era of Radarsat/ERS-2 missions. These include a new data acquisition planning capability to manage more satellites with global planning and to manage more than one instrument mode; a new archive strategy that is cheaper, faster, and better; a product generation system to produce data on demand and to produce data for the varied instruments and modes; and a product verification ability for the new and old products. In response to these new functional requirements, JPL is using a design approach that emphasizes an open systems, client/server architecture based on industry standards and commercial off-the-shelf technology.

Radarsat/ERS-2 ground data system upgrade↗

NASA NTRS1974

Damping synthesis from substructure tests

A new method is proposed for synthesizing structural damping from substructure test data. It utilizes the off-diagonal coupling terms in the substructure modal damping matrices as well as the diagonal terms which correspond to uncoupled modal damping. The coupling terms are evaluated from complex resonant response measured at each substructure mode. Both analytical and experimental verification of the method have been made. Accuracy requirements on experimental data and data reduction have been established.

Hasselman, T. K.↗

NASA NTRS1978

Data screening and preprocessing for Landsat MSS data

Two computer algorithms are presented. The first, called SCREEN, is used to automatically identify pixels representing clouds, cloud shadows, snow, water, or anomalous signals in Landsat-2 data. The second, called XSTAR, compensates Landsat-2 data for the effects of atmospheric haze, without requiring ground measurements or ground references. The presentation of these algorithms includes their theoretical background, algebraic details, and performance characteristics. Verification of the algorithms has for the present been limited to Landsat agricultural data. Plans for further development of the XSTAR technique are also presented.

Lambeck, P. F.↗

NASA NTRS2008

Guidance and Control Software Project Data - Volume 2: Development Documents

The Guidance and Control Software (GCS) project was the last in a series of software reliability studies conducted at Langley Research Center between 1977 and 1994. The technical results of the GCS project were recorded after the experiment was completed. Some of the support documentation produced as part of the experiment, however, is serving an unexpected role far beyond its original project context. Some of the software used as part of the GCS project was developed to conform to the RTCA/DO-178B software standard, "Software Considerations in Airborne Systems and Equipment Certification," used in the civil aviation industry. That standard requires extensive documentation throughout the software development life cycle, including plans, software requirements, design and source code, verification cases and results, and configuration management and quality control data. The project documentation that includes this information is open for public scrutiny without the legal or safety implications associated with comparable data from an avionics manufacturer. This public availability has afforded an opportunity to use the GCS project documents for DO-178B training. This report provides a brief overview of the GCS project, describes the 4-volume set of documents and the role they are playing in training, and includes the development documents from the GCS project. Volume 2 contains three appendices: A. Guidance and Control Software Development Specification; B. Design Description for the Pluto Implementation of the Guidance and Control Software; and C. Source Code for the Pluto Implementation of the Guidance and Control Software

Hayhurst, Kelly J.↗

NASA NTRS2008

Guidance and Control Software Project Data - Volume 4: Configuration Management and Quality Assurance Documents

The Guidance and Control Software (GCS) project was the last in a series of software reliability studies conducted at Langley Research Center between 1977 and 1994. The technical results of the GCS project were recorded after the experiment was completed. Some of the support documentation produced as part of the experiment, however, is serving an unexpected role far beyond its original project context. Some of the software used as part of the GCS project was developed to conform to the RTCA/DO-178B software standard, "Software Considerations in Airborne Systems and Equipment Certification," used in the civil aviation industry. That standard requires extensive documentation throughout the software development life cycle, including plans, software requirements, design and source code, verification cases and results, and configuration management and quality control data. The project documentation that includes this information is open for public scrutiny without the legal or safety implications associated with comparable data from an avionics manufacturer. This public availability has afforded an opportunity to use the GCS project documents for DO-178B training. This report provides a brief overview of the GCS project, describes the 4-volume set of documents and the role they are playing in training, and includes configuration management and quality assurance documents from the GCS project. Volume 4 contains six appendices: A. Software Accomplishment Summary for the Guidance and Control Software Project; B. Software Configuration Index for the Guidance and Control Software Project; C. Configuration Management Records for the Guidance and Control Software Project; D. Software Quality Assurance Records for the Guidance and Control Software Project; E. Problem Report for the Pluto Implementation of the Guidance and Control Software Project; and F. Support Documentation Change Reports for the Guidance and Control Software Project.

Hayhurst, Kelly J.↗

NASA NTRS2015

Wilson Corners SWMU 001 2014 Annual Long Term Monitoring Report Kennedy Space Center, Florida

This document presents the findings of the 2014 Long Term Monitoring (LTM) that was completed at the Wilson Corners site, located at the National Aeronautics and Space Administration (NASA) John F. Kennedy Space Center (KSC), Florida. The goals of the 2014 annual LTM event were to evaluate the groundwater flow direction and gradient and to monitor the vertical and downgradient horizontal extent of the volatile organic compounds (VOCs) in groundwater at the site. The LTM activities consisted of an annual groundwater sampling event in December 2014, which included the collection of water levels from the LTM wells. During the annual groundwater sampling event, depth to groundwater was measured and VOC samples were collected using passive diffusion bags (PDBs) from 30 monitoring wells. In addition to the LTM sampling, additional assessment sampling was performed at the site using low-flow techniques based on previous LTM results and assessment activities. Assessment of monitoring well MW0052DD was performed by collecting VOC samples using low-flow techniques before and after purging 100 gallons from the well. Monitoring well MW0064 was sampled to supplement shallow VOC data north of Hot Spot 2 and east of Hot Spot 4. Monitoring well MW0089 was sampled due to its proximity to MW0090. MW0090 is screened in a deeper interval and had an unexpected detection of trichloroethene (TCE) during the 2013 LTM, which was corroborated during the March 2014 verification sampling. Monitoring well MW0130 was sampled to provide additional VOC data beneath the semi-confining clay layer in the Hot Spot 2 area.

Monitoring↗

NASA NTRS1978

The classification of LANDSAT data for the Orlando, Florida, urban fringe area

Procedures used to map residential land cover on the Orlando, Florida, Urban fringe zone are detailed. The NASA Bureau of the Census Applications Systems Verification and Transfer project and the test site are described as well as the LANDSAT data used as the land cover information sources. Both single-date LANDSAT data processing and multitemporal principal components LANDSAT data processing are described. A summary of significant findings is included.

Walthall, C. L.↗

NASA NTRS1973

A semi-automated system for plotting and computer cataloging of remote sensing imagery

Description of a computer-assisted system for plotting and descriptor-cataloging of aerial imagery - an ingredient of the aerial image data handling system of the NASA Earth Resources Aircraft Project (ERAP). The system under consideration uses two graphic tablets linked interactively with a digital computer as input devices for deriving frame coordinates by correlating image points to map points. The various software programs and operating modes facilitate a semiautomatic plotting of an image by selecting two or more points within the image for matching with map details. The computer performs the scaling and orientation transformations for the calculation of the center and corner coordinates of the frame. Twenty five other descriptors for each frame are fed into a computerized data base requiring a minimum operator's time for verification and correction.

Gnauck, G. E.↗

NASA NTRS1978

An investigation of heat pipe meniscus heat transfer

The use of grooved evaporator surfaces in heat pipes has increased in popularity in the past few years primarily due to the reproducibility achievable with grooved walls and the relatively low costs of the threading or extrusion processes involved in their production. The present study combines both analyses and experiments on square groove geometries, with special emphasis on overcoming the limitations of earlier analyses with finite-difference methods and groove-fillet hydrodynamic simplifications. The groove fillet, which has in previous analyses been assumed constant in radius of curvature, is permitted to change in thickness and curvature consistent with hydrodynamics and heat loss from the groove. A model is developed for accurate determination of the effect of constriction resistance on groove performance. The grooved-surface tests to be conducted are briefly described which will provide data under closely controlled operation to allow comparison and verification of the analyses.

Saaski, E. W.↗

NASA NTRS1978

Advanced rotorcraft technology: Task force report

The technological needs and opportunities related to future civil and military rotorcraft were determined and a program plan for NASA research which was responsive to the needs and opportunities was prepared. In general, the program plan places the primary emphasis on design methodology where the development and verification of analytical methods is built upon a sound data base. The four advanced rotorcraft technology elements identified are aerodynamics and structures, flight control and avionic systems, propulsion, and vehicle configurations. Estimates of the total funding levels that would be required to support the proposed program plan are included.

Source record↗

NASA NTRS1981

Determination of load sequence effects on the degradation and failure of composite materials

A theoretical model was established to predict the fatigue behavior of composite materials, with emphasis placed on predictions of the degradation of residual strength and residual stiffness during fatigue cycling. The model parameters were evaluated from three test series including static strength fatigue life and residual strength tests. The tests were applied to two graphite/epoxy laminates. Load sequence effects were emphasized for both laminates and the predicted results agreed quite well with subsequent verification tests. Dynamic as well as static stiffness reduction data were collected by use of a PDP11-03 computer, which performed quite satisfactorily and permitted the recording of a substantial amount of dynamic stiffness reduction data.

Yang, J. N.↗

NASA NTRS1981

NASA/BLM APT, phase 2. Volume 2: Technology demonstration

Techniques described include: (1) steps in the preprocessing of LANDSAT data; (2) the training of a classifier; (3) maximum likelihood classification and precision; (4) geometric correction; (5) class description; (6) digitizing; (7) digital terrain data; (8) an overview of sample design; (9) allocation and selection of primary sample units; (10) interpretation of secondary sample units; (11) data collection ground plots; (12) data reductions; (13) analysis for productivity estimation and map verification; (14) cost analysis; and (150) LANDSAT digital products. The evaluation of the pre-inventory planning for P.J. is included.

Source record↗