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At least 667 records · Page 37

Survival of B. Horneckiae Spores Under Ground-simulated Space Conditions

To prevent forward contamination and maintain the scientific integrity of future life detection missions, it is important to characterize and attempt to eliminate terrestrial microorganisms associated with exploratory spacecraft and landing vehicles. Among the organisms isolated from spacecraft-associated habitats, spore-forming microbes are highly resistant to various physical and chemical conditions, which include ionizing and UV radiation, desiccation and oxidative stress, and the harsh environment of outer space or planetary surfaces. Recently a radiation resistant, spore forming bacterial isolate, Bacillus horneckiae, was isolated from a clean room of the Kennedy Space Center where the Phoenix spacecraft was assembled. The exceptionally high tolerance of extreme conditions demonstrated by sporeforming bacteria highlighted the need to assess the viability of these microbes in situ (in real) space. The proposed BOSS (Biofilm Organisms Surfing Space) project aims to understand the mechanisms by which biofilm forming organisms, such as B. horneckiae, will potentially be able to withstand harsh space conditions. As previously stated, the spore producing ability of these species gives them increased survivability to harsh conditions. Some of the spores will have the protective exosporium layer artificially removed before the test to determine if the existence of this layer significantly changes the survivability during the mission. In preparation for that experiment, we analyzed spores which were exposed during a ground simulation, the EXPOSE R2 Biofilm Organisms Surfing Space (BOSS). Previous to exposure, spores were deposited onto spacecraft grade aluminum coupons in a spore suspension calculated to contain between 10(exp 7) and 10(exp 8) spores. This precursor series will be used to establish a baseline survivability function for comparison with the future flight tests during EXPOSE-R. For each coupon, a 10% polyvinyl alcohol (PVA) film was applied and peeled from the coupon to recover the spores. One hundred μl of sterile 10% PVA was applied to the surface of the coupon and allowed to dry for 1 hour at 37 C. The films were then removed using sterile scalpel and forceps and placed into a glass test tube containing 2 milliliters of sterile deionized water. The PVA film process was then repeated on each coupon one additional time to ensure recovery of the majority of spores. The second PVA film was added in the same glass tube as in the previous round. If the spores remained 100% viable, the test tubes should now contain between 5 X 10(exp 6) and 5 X 10(exp 7) spores per millimeter; however, it is expected that some loss of viability has occurred. In order to assess this loss, the number of colony forming, viable spores was counted. To count the colony forming units (CFUs), the spore containing solution was diluted in a process of 10-fold serial dilution by mixing successive solutions in a 100 microliter spore suspension to 900 microliter deionized H2O ratio. A sample dilution series revealed that 10(exp -3) and 10(exp -4) concentrations would be necessary for an accurate CFU count to be taken. For those two concentrations, a spread on a TSA plate was prepared and incubated at 32 C. For the samples exposed to UV radiation, the cell survivability was too low to establish a count from 100 microliter spread plating. Instead, no dilutions were performed and the entire 2 milliliter spore suspension was plated and incubated at 32 C. The plate's CFU counts were taken at 24 hours and 48 hours from the time of plating. At the end of the CFU counting the total surviving spores in each sample were calculated based on the number of CFUs that were observed per 100 microliters, or per 2 milliliters for the UV irradiated samples. The results of these calculations are shown in Figures 1 and 2.

cleanroom↗

Earthquake detection in a simulated lunar regolith using distributed acoustic sensing

Current models of inner lunar geology have largely been inferred from the seismic experiments and observations performed during the Apollo missions that comprised a relatively small number of seismic instruments. Refining constraints on fundamental lunar relationships such as crust-mantle and mantle-core boundaries in the future will require seismic arrays spanning larger epicentral distances. A promising technology for installing dense seismic arrays with minimal human effort is distributed acoustic sensing (DAS), an approach that allows a single length of fiber optic cable to act as hundreds or thousands of sensors when coupled with a DAS interrogator. While terrestrial uses of DAS technology for seismic monitoring rely on burying the cable to maximize fidelity of seismic signal transmission to the fiber, digging meters of trench to bury optical fiber on lunar or planetary surfaces is logistically infeasible. To evaluate DAS signal attenuation due to surface deployment of cable in lunar regolith, we completed earthquake detection analyses that evaluated the sensitivity of an optic-fiber DAS system to seismic signals at different burial depths. We deployed a single-mode fiber in a 10-m open-bottom wooden box filled with a lunar regolith simulant (LRS) with fiber buried at different depths within the LRS and recorded signals for four regional and local earthquakes. The results were used to identify and evaluate signal attenuation in surface-deployed fiber compared to buried fiber in the LRS. Burial depth responses to active-source signals were also evaluated similar to previous studies characterizing DAS sensitivity of surface-deployed fiber. Atmospheric noise was minimal as the cable was deployed in an indoor environment; however, where observed, atmospheric and anthropogenic noise was filtered out using the same bandpass filtering used to identify earthquake events. We found that signal attenuation of the surface-deployed fiber compared to buried fiber was relatively high in active-source experiments but was not consistently observed in earthquake signals. That burial depth is not highly correlated to attenuation of the observed earthquake signals indicates that in a noise-limited environment, placing DAS-interrogated fiber directly at the regolith surface may be a promising deployment strategy to consider for sensing remote seismic signals during lunar exploration.

58 GEOSCIENCES↗

Evidence of non-isentropic release from high residual temperatures in shocked metals measured with ultrafast x-ray diffraction

Shock experiments are widely used to understand the mechanical and electronic properties of matter under extreme conditions. However, after shock loading to a Hugoniot state, a clear description of the post-shock thermal state and its impacts on materials is still lacking. We used diffraction patterns from 100-fs x-ray pulses to investigate the temperature evolution of laser-shocked Al–Zr metal film composites at time delays ranging from 5 to 75 ns driven by a 120-ps short-pulse laser. We found significant heating of both Al and Zr after shock release, which can be attributed to heat generated by inelastic deformation. A conventional hydrodynamic model that employs (i) typical descriptions of Al and Zr mechanical strength and (ii) elevated strength responses (which might be attributed to an unknown strain rate dependence) did not fully account for the measured temperature increase, which suggests that other strength-related mechanisms (such as fine-scale void growth) could play an important role in thermal responses under shock wave loading/unloading cycles. Our results suggest that a significant portion of the total shock energy delivered by lasers becomes heat due to defect-facilitated plastic work, leaving less converted to kinetic energy. This heating effect may be common in laser-shocked experiments but has not been well acknowledged. High post-shock temperatures may induce phase transformation of materials during shock release. Another implication for the study is the preservability of magnetic records from planetary surfaces that have a shock history from frequent impact events.

42 ENGINEERING↗

Foreword Special issue on the Kilopower Project, Kilowatt Reactor Using Stirling TechnologY (KRUSTY) Test

This special issue of Nuclear Technology contains full-length, peer-reviewed papers describing the design work leading up to and the results of the Kilowatt Reactor Using Stirling TechnologY (KRUSTY) test. KRUSTY was the centerpiece of the National Aeronautics and Space Administration (NASA) Kilopower Project to design, build, and test a space nuclear reactor. This test was the first such test since the end of the Space Nuclear Auxiliary Power (SNAP) project at the end of the 1960s. In this issue, the introduction paper presents the goals of the Kilopower Project and the potential missions this reactor concept could serve in NASA. Kilopower was intended to serve both human exploration needs on planetary surfaces as well as science needs for deep-space exploration. The design work for the experiment by Poston and the power conversion development by Gibson present the pre-work required to perform the eventual KRUSTY test. A paper on regulatory analysis follows, to show the path used to gain approval of the proposed experiment. Then, the early zero-power critical experiments are presented by Sanchez and Grove. These experiments were essential data used to enhance model predictions prior to the high-temperature test. Next, Poston presents the three experiments (warm criticals) that increase the temperature in an incremental fashion prior to the final experiment. These experiments were used to achieve final regulatory approval of the final high-temperature experiment. The last paper by Poston presents the results of the steady-state and transient testing of the reactor at full power and at the design temperature. These results show that the reactor design and as-built experiment met all of the requirements that NASA had developed for the system. Any experiment of this magnitude was accomplished only because of the hard work and dedication of a large number of people at multiple institutions, including the NASA Glenn Research Center, NASA Marshall Space Flight Center, Y-12 National Security Site, Los Alamos National Laboratory, and the Nevada National Security Site prime contractor (formerly National Security Technologies and now the Mission Support and Test Services). The project was jointly funded by the Space Technology Mission Directorate at NASA and the Criticality Safety Program at the National Nuclear Security Administration (NNSA). A special thanks to Jerry McKamy and Angela Chambers at the NNSA and Lee Mason and Don Palac at NASA for their support and guidance. For the authors and multitude of staff that performed this work, the passion for space nuclear reactors was the key to making this experiment a reality.

99 GENERAL AND MISCELLANEOUS↗

Detection of visible-wavelength aurora on Mars

Mars hosts various auroral processes despite the planet’s tenuous atmosphere and lack of a global magnetic field. To date, all aurora observations have been at ultraviolet wavelengths from orbit. We describe the discovery of green visible-wavelength aurora, originating from the atomic oxygen line at 557.7 nanometers, detected with the SuperCam and Mastcam-Z instruments on the Mars 2020 Perseverance rover. Near–real-time simulations of a Mars-directed coronal mass ejection (CME) provided sufficient lead-time to schedule an observation with the rover. The emission was observed 3 days after the CME eruption, suggesting that the aurora was induced by particles accelerated by the moving shock front. To our knowledge, detection of aurora from a planetary surface other than Earth has never been reported, nor has visible aurora been observed at Mars. This detection demonstrates that auroral forecasting at Mars is possible, and that during events with higher particle precipitation, or under less dusty atmospheric conditions, aurorae will be visible to future astronauts.

Science & Technology - Other Topics↗

River sinuosity describes a continuum between randomness and ordered growth

River channels are among the most common landscape features on Earth. An essential characteristic of channels is sinuosity: their tendency to take a circuitous path, which is quantified as along-stream length divided by straight-line length. River sinuosity is interpreted as a characteristic that either forms randomly at channel inception or develops over time as meander bends migrate. Studies tend to assume the latter and thus have used river sinuosity as a proxy for both modern and ancient environmental factors including climate, tectonics, vegetation, and geologic structure. But no quantitative criterion for planform expression has distinguished between random, initial sinuosity and that developed by ordered growth through channel migration. This ambiguity calls into question the utility of river sinuosity for understanding Earth’s history. We propose a quantitative framework to reconcile these competing explanations for river sinuosity. Using a coupled analysis of modeled and natural channels, we show that while a majority of observed sinuosity is consistent with randomness and limited channel migration, rivers with sinuosity ≥1.5 likely formed their geometry through sustained, ordered growth due to channel migration. This criterion frames a null hypothesis for river sinuosity that can be applied to evaluate the significance of environmental interpretations in landscapes shaped by rivers. The quantitative link between sinuosity and channel migration further informs strategies for preservation and restoration of riparian habitat and guides predictions of fluvial deposits in the rock record and in remotely sensed environments from the seafloor to planetary surfaces.

58 GEOSCIENCES↗

OrganiCam: a lightweight time-resolved laser-induced luminescence imager and Raman spectrometer for planetary organic material characterization

OrganiCam is a laser-induced luminescence imager and spectrometer designed for standoff organic and biosignature detection on planetary bodies. OrganiCam uses a diffused laser beam (12° cone) to cover a large area at several meters distance and records luminescence on half of its intensified detector. The diffuser can be removed to record Raman and fluorescence spectra from a small spot from 2 m standoff distance. OrganiCam’s small size and light weight makes it ideal for surveying organics on planetary surfaces. Here, we have designed and built a brassboard version of the OrganiCam instrument and performed initial tests of the system.

47 OTHER INSTRUMENTATION↗

Dynamical Inference and 3D Imaging of Magnetized Dusty Plasmas

This is the final technical report for this DOE award. The motion of dust particles in a laboratory plasma has been studied for nearly 30 years and has led to the discovery of strongly-coupled crystalline structures and nonequilibrium dynamics governed by gravitational, hydrodynamic, and electrostatic forces. Many of the inter-particle forces are complex; they can be non-reciprocal, and non-additive. Deciphering the mélange of particle interactions has been a challenging task, nevertheless, magnetized dusty plasmas remains an open challenge with limited understanding. With applications ranging from magnetically-confined plasmas for fusion to near-surface planetary environments, this research field is ripe for well-controlled, laboratory experiments and new theoretical tools. In collaboration with the MDPX experiment at Auburn University, this proposal aims to tease apart both the known and unknown forces that drive dusty plasmas in magnetized environments using high-resolution, three-dimensional imaging and particle tracking coupled with modern dynamical inference and machine learning techniques.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Accretion Properties of PDS 70b with MUSE

We report a new evaluation of the accretion properties of PDS 70b obtained with the Very Large Telescope/Multi Unit Spectroscopic Explorer. The main difference from the previous studies of Haffert et al. and Aoyama & Ikoma is in the mass accretion rate. Simultaneous multiple line observations, such as Hα and Hβ, can better constrain the physical properties of an accreting planet. While we clearly detected Hα emissions from PDS 70b, no Hβ emissions were detected. We estimate the line flux of Hβ with a 3σ upper limit to be 2.3 × 10{sup −16} erg s{sup −1} cm{sup −2}. The flux ratio F {sub Hβ}/F {sub Hα} for PDS 70b is <0.28. Numerical investigations by Aoyama et al. suggest that F {sub Hβ}/F {sub Hα} should be close to unity if the extinction is negligible. We attribute the reduction of the flux ratio to the extinction, and estimate the extinction of Hα (A {sub Hα}) for PDS 70b to be >2.0 mag using the interstellar extinction value. By combining with the Hα linewidth and the dereddening line luminosity of Hα, we derive the PDS 70b mass accretion rate to be ≳5 × 10{sup −7} M {sub Jup} yr{sup −1}. The PDS 70b mass accretion rate is an order of magnitude larger than that of PDS 70. We found that the filling factor f {sub f} (the fractional area of the planetary surface emitting Hα) is ≳0.01, which is similar to the typical stellar value. The small value of f {sub f} indicates that the Hα emitting areas are localized at the surface of PDS 70b.

79 ASTRONOMY AND ASTROPHYSICS↗

A New Martian Crater Chronology: Implications for Jezero Crater

Crater chronologies are a fundamental tool to assess the relative and absolute ages of planetary surfaces when direct radiometric dating is not available. Martian crater chronologies are derived from lunar crater spatial densities on terrains with known radiometric ages, and thus they critically depend on the Moon-to-Mars extrapolation. This extrapolation requires knowledge of the time evolution of the impact flux, including contributions from various impactor populations, factors that are not trivially connected to the dynamical evolution of the early Solar System. In this paper, we will present a new Martian crater chronology based on current dynamical models, and consider the main sources of uncertainties (e.g., impactor size–frequency distribution; dynamical models with late and early instabilities, etc.). The resulting “envelope” of Martian crater chronologies significantly differs from previous chronologies. The new Martian crater chronology is discussed using two interesting applications: Jezero crater’s dark terrain (relevant to the NASA Mars 2020 mission) and the southern heavily cratered highlands. Our results indicate that Jezero’s dark terrain may have formed ∼3.1 Ga, i.e., up to 0.5 Gyr older than previously thought. In addition, available crater chronologies (including our own) overestimate the number of craters larger than 150 km on the southern highlands, suggesting either that large craters have been efficiently erased over Martian history or that dynamical models need further refinement. Further, our chronology constrains the age of Isidis basin to be 4.05–4.2 Ga and that of the Borealis basin to be 4.35–4.40 Ga; these are predictions that can be tested with future sample and return missions.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Automated microbial metabolism laboratory.

Experiments integrated into single automated laboratory to detect extraterrestrial life through measuring metabolism and growth in planetary surface material

Levin, G. V.↗

Extraterrestrial life detection.

Sequential analyses of planetary surface sample for extraterrestrial life detection, discussing chemistry, morphology, growth and metabolism for life attributes

Young, R. S.↗