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At least 667 records · Page 37
All sourcesNASA NTRSDOE OSTI
NASA NTRS1994

Experiment K-7-17: Effects of Spaceflight on the Proliferation of Jejunal Mucosal Cells

The purpose of this project was to test the hypothesis that the generalized, whole body decrease in synthetic activity due to microgravity conditions encountered during spaceflight would be demonstrable in cells and tissues characterized by a rapid rate of turnover. Jejunal mucosal cells were chosen as a model since these cells are among the most rapidly proliferating in the body. Accordingly, the percentage of mitotic cells present in the crypts of Lieberkuhn in each of 5 rats flown on the COSMOS 2044 mission were compared to the percentage of mitotic cells present in the crypts in rats included in each of 3 ground control groups (i.e., vivarium, synchronous and caudal-elevated). No significant difference (p greater than .05) was detected in mitotic indices between the flight and vivarium group. Although the ability of jejunal mucosal cells to divide by mitosis was not impaired in flight group, there was, however, a reduction in the length of villi and depth of crypts. The concommitant reduction in villus length and crypth depth in the flight group probably reflects changes in connective tissue components within the core of villi.

Phillips, R. W.↗
NASA NTRS1996

Lessons learned: DC-X

The DC-X was conceived and developed specifically to lay the ground work for significantly lowering the cost of space operations. The system design was based on an initial set of program goals and a finite, limited set of resources. The goal in its simplest terms was to demonstrate vertical landing after rotation of the vehicle from a nose-first to an engines-first altitude. Finite resources actually drove the selection of a robust design to reduce fabrication and preflight testing costs. The result was a system with a large amount of flexibility which allowed expansion of the test goals as the system, and test program, evolved. The use of the vehicle flight computer interfacing with the ground control system for flight crew training was also not an initial concept. However, by defining an architecture for the system control modes which allowed additions and modifications as learning progressed, the 6 DOF codes used for flight controls software development were transported to the operating system to be used in a simulated flight mode. Flight data reduction was also greatly improved as the program progressed, and the data needs and presentation were refined. The software, avionics hardware, and the FOCC system development proceeded ahead of the vehicle, primarily because most of the hardware elements were existing at the outset of the program. The Built-in-Test (BIT) for avionics and propulsion systems were adequate. Particularly the flight readiness system which verified the vehicle health after engine start and before throttle-up for flight.

Steinmeyer, D. A.↗
NASA NTRS1996

Fault Diagnosis of Power Systems Using Intelligent Systems

The power system operator's need for a reliable power delivery system calls for a real-time or near-real-time Al-based fault diagnosis tool. Such a tool will allow NASA ground controllers to re-establish a normal or near-normal degraded operating state of the EPS (a DC power system) for Space Station Alpha by isolating the faulted branches and loads of the system. And after isolation, re-energizing those branches and loads that have been found not to have any faults in them. A proposed solution involves using the Fault Diagnosis Intelligent System (FDIS) to perform near-real time fault diagnosis of Alpha's EPS by downloading power transient telemetry at fault-time from onboard data loggers. The FDIS uses an ANN clustering algorithm augmented with a wavelet transform feature extractor. This combination enables this system to perform pattern recognition of the power transient signatures to diagnose the fault type and its location down to the orbital replaceable unit. FDIS has been tested using a simulation of the LeRC Testbed Space Station Freedom configuration including the topology from the DDCU's to the electrical loads attached to the TPDU's. FDIS will work in conjunction with the Power Management Load Scheduler to determine what the state of the system was at the time of the fault condition. This information is used to activate the appropriate diagnostic section, and to refine if necessary the solution obtained. In the latter case, if the FDIS reports back that it is equally likely that the faulty device as 'start tracker #1' and 'time generation unit,' then based on a priori knowledge of the system's state, the refined solution would be 'star tracker #1' located in cabinet ITAS2. It is concluded from the present studies that artificial intelligence diagnostic abilities are improved with the addition of the wavelet transform, and that when such a system such as FDIS is coupled to the Power Management Load Scheduler, a faulty device can be located and isolated from the rest of the system. The benefit of these studies provides NASA with the ability to quickly restore the operating status of a space station from a critical state to a safe degraded mode, thereby saving costs in experimentation rescheduling, fault diagnostics, and prevention of loss-of-life.

Momoh, James A.↗
NASA NTRS1996

STS-80 Flight Day 2

On this second day of the STS-80 mission, the flight crew, Cmdr. Kenneth D. Cockrell, Pilot Kent V. Rominger, Mission Specialists, Tamara E. Jernigan, Thomas D. Jones, and F. Story Musgrave, complete the first major objective of the mission with the deployment of the Orbiting Retrievable Far and Extreme Ultraviolet Spectrometer (ORFEUS) on the reusable Shuttle Pallet Satellite. Release of ORFEUS from Columbia's robot arm came at 8 hours 15 minutes mission elapsed time. Three hours after the release, ground controllers inform the crew that the instrument package appears to be working properly. This begins two weeks of gathering data on the origin and makeup of stars.

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NASA NTRS1996

STS-80 Flight Day 12

On this twelfth day of the STS-80 mission, the flight crew, Cmdr. Kenneth D. Cockrell, Pilot Kent V. Rominger, Mission Specialists, Tamara E. Jernigan, Thomas D. Jones, and F. Story Musgrave, spend the day discussing the failed hatch with ground controllers. The failure of the hatch to properly open causes the cancellation of the second planned spacewalk by Jernigan and Jones. NASA engineers and managers continue to collect and analyze data on what may be causing the failure. The leading candidate is a misalignment of the hatch against the airlock seal.

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NASA NTRS1993

Morphological Changes in Rat Vestibular System Following Weightlessness

Mammalian gravity receptors (maculas) are morphologically organized for weighted, parallel distributed processing of information. There are two basic circuits: 1) highly channeled, type I cell to calyx; and 2) distributed modifying, type II cells to calyces and processes. The latter circuit should be the more adaptable since it modifies final output. To test this hypothesis, rats were flown in microgravity for 9 days aboard a space shuttle and euthanized shortly after landing. Hair cells and ribbon synapses from maculas of 3 flight and 3 ground control rats were studied ultrastructurally in blocks of 50 serial sections. Synapses increased by approximately 41% in type I cells and by approximately 55% in type II cells in flight animals. There was a shift toward the spherular form of ribbon synapse in both types of hair cells in flight animals. Current findings tend to support the stated hypothesis and indicate that mature utricular hair cells retain synaptic plasticity, permitting adaptation to an altered gravitational environment.

Ross, Muriel D.↗
NASA NTRS1997

STS-82 Flight Day 04 Highlights

The fourth day of the STS-82 mission begins with the crew, Commander Kenneth D. Bowersox, Pilot Scott J. Horowitz, Payload Commander Mark C. Lee, and Mission Specialists Gregory J. Harbaugh, Steven L. Smith, Joseph R. Tanner, and Steven A. Hawley in preparations for conducting the second servicing mission of the Hubble Space Telescope. The first spacewalk was slightly delayed to enable ground controllers to assess the unexpected movement of one of Hubble's solar arrays, which slewed from a horizontal to a vertical position as Discovery's airlock was depressurized. Astronauts Mark Lee and Steve Smith are seen working in the cargo bay of the Shuttle Discovery. Their spacewalk to upgrade the Hubble Space Telescope lasts six hours and 42 minutes. At the conclusion of their EVA, HST has graded science instruments for an expanded view of the universe.

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NASA NTRS1993

Demonstration of a High-Fidelity Predictive/Preview Display Technique for Telerobotic Servicing in Space

A highly effective predictive/preview display technique for telerobotic servicing in space under several seconds communication time delay has been demonstrated on a large laboratory scale in May 1993, involving the Jet Propulsion Laboratory as the simulated ground control station and, 2500 miles away, the Goddard Space Flight Center as the simulated satellite servicing set-up. The technique is based on a high-fidelity calibration procedure that enables a high-fidelity overlay of 3-D graphics robot arm and object models over given 2-D TV camera images of robot arm and objects. To generate robot arm motions, the operator can confidently interact in real time with the graphics models of the robot arm and objects overlaid on an actual camera view of the remote work site. The technique also enables the operator to generate high-fidelity synthetic TV camera views showing motion events that are hidden in a given TV camera view or for which no TV camera views are available. The positioning accuracy achieved by this technique for a zoomed-in camera setting was about +/-5 mm, well within the allowable +/-12 mm error margin at the insertion of a 45 cm long tool in the servicing task.

Kim, Won S.↗
NASA NTRS1996

Experimental Modification of Rat Pituitary Growth Hormone Cell Function During and After Spaceflight

Space-flown rats show a number of flight-induced changes in the structure and function of pituitary Growth Hormone (GH) cells after in vitro postflight testing. To evaluate the possible effects of microgravity on GH cells themselves, freshly dispersed rat anterior pituitary gland cells were seeded into vials containing serum +/- 1 micron HydroCortisone (HC) before flight. Five different cell preparations were used: the entire mixed-cell population of various hormone-producing cell types, cells of density less than 1.071 g/sq cm (band 1), cells of density greater than 1.071 g/sq cm (band 2), and cells prepared from either the dorsal or ventral part of the gland. Relative to ground control samples, bioactive GH released from dense cells during flight was reduced in HC-free medium but was increased in HC-containing medium. Band I and mixed cells usually showed opposite HC-dependent responses. Release of bioactive GH from ventral flight cells was lower; postflight responses to GH-releasing hormone challenge were reduced, and the cytoplasmic area occupied by GH in the dense cells was greater. Collectively, the data show that the chemistry and cellular makeup of the culture system modifies the response of GH cells to microgravity. As such, these cells offer a system to identify gravisensing mechanisms in secretory cells in future microgravity research.

Hymer, W. C.↗
NASA NTRS1996

Experimental Modification of Rat Pituitary Prolactin Cell Function During and After Spaceflight

Experimental modification of rat pituitary prolactin cell function during and after spaceflight. This study was done to evaluate the effects of microgravity on prolactin (PRL) cells of the male rat pituitary gland. We used the identical passive closed-vial cell culture system that was described for the culture of growth hormone cells (W C. Hymer, R. E. Grindeland, T. Salada, P. Nye, E. Grossman, and R Lane). After an 8-day spaceflight, all flight media (containing released PRL), as well as extracts (containing intracellular PRL), contained significantly lower amounts of immunoreactive PRL than their corresponding ground control samples. On the other hand, these same samples, when assessed for their biological activities by two different in vitro lymphocyte assays, yielded disparate results that may reflect posttranslational modifications to the hormone molecule. Other data showed that: (1) the apparent molecular weights of released PRL molecules were not altered by microgravity; but (2) the region from which the PRL cells came (dorsal or ventral) made a significant difference in the amount and activity of PRL released from the flight cells. Because there is much current interest in the role that PRL may play in the regulation of the immune system and because changes in both cellular and humoral immunity accompany spaceflight, this study could help define future microgravity research in this area.

Hymer, W. C.↗
NASA NTRS1995

STS-94 Day 12 Highlights

On this twelfth day of the STS-94 mission, the flight crew, Cmdr. James D. Halsell, Jr., Pilot Susan L. Still, Payload Cmdr. Janice E. Voss, Mission Specialists Michael L. Gernhardt and Donald A. Thomas, and Payload Specialists Gregory T. Linteris and Roger K. Crouch focus on developing better methods for the efficient use of fossil fuels while reducing emissions and air pollutants. The seven-astronaut crew -- divided into two teams -- provides on-orbit assistance to ground controllers throughout the mission conducting these, and as many as 30 other experiments in the Spacelab pressurized module. The goal is to emulate what laboratory work will be like on the future International Space Station.

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NASA NTRS1997

Evaluation of Optimum HgZnTe Crystal Growth Parameters and USML-2 Flight Support

Work associated with contract provided support for the approved flight experiment 'Crystal Growth of Selected 2-6 Semiconducting Alloys by Directional Solidification.' General activities included ground-based melt studies, compositional and microstructure characterization of the grown crystals, and participation in the continuing assessment of the program. Specifically, work was performed to determine the optimum furnace translation rate at which a HgZnTe sample could be removed from the crystal growth furnace to avoid shrinkage cavities. Additionally, nine HgZnTe ampoules were prepared and delivered to Marshall Space Flight Center for Ground Control Experiment Laboratory Testing.

Scripa, Rosalia N.↗
NASA NTRS1997

Regulation of Blood Volume During Spaceflight

The effects of spaceflight on erythropoiesis and blood volume in the rat were studied during the 14-day NASA Spacelab Life Sciences 2 (SLS-2) Shuttle mission. Measurements included red blood cell mass (RBCM), plasma volume (PV), iron utilization and iron utilization in response to an injection of erythropoietin. Red blood cell (RBC) survival, splenic sequestration and erythrocyte morphology were also evaluated. At landing, the RBCM adjusted for body weight was significantly lower in the flight animals than in the ground controls. While the PV was also decreased, the change was not statistically significant. Incorporation of iron into circulating RBCs was normal when measured after five days of spaceflight and the rat responded normally to the single in-flight injection of erythropoietin. No change in RBC morphology could be attributed to spaceflight. A normal survival was found for the RBC population that was represented by Cr-51 labeled RBCS. These results demonstrate that rats, like humans, return from spaceflight with a decreased RBCM and total blood volume.

Alfrey, Clarence P.↗
NASA NTRS1998

Flight Demonstration of Integrated Airport Surface Movement Technologies

This document describes operations associated with a set of flight experiments and demonstrations using a Boeing-757-200 research aircraft as part of low visibility landing and surface operations (LVLASO) research activities. To support this experiment, the B-757 performed flight and taxi operations at the Atlanta Hartsfield International Airport in Atlanta, GA. The test aircraft was equipped with experimental displays that were designed to provide flight crews with sufficient information to enable safe, expedient surface operations in any weather condition down to a runway visual range of 300 feet. In addition to flight deck displays and supporting equipment onboard the B-757, there was also a ground-based component of the system that provided for ground controller inputs and surveillance of airport surface movements. Qualitative and quantitative results are discussed.

Young, Steven D.↗
NASA NTRS1997

A geostationary satellite system for mobile multimedia applications using portable, aeronautical and mobile terminals

A geostationary satellite system for mobile multimedia services via portable, aeronautical and mobile terminals was developed within the framework of the Advanced Communications Technology Service (ACTS) programs. The architecture of the system developed under the 'satellite extremely high frequency communications for multimedia mobile services (SECOMS)/ACTS broadband aeronautical terminal experiment' (ABATE) project is presented. The system will be composed of a Ka band system component, and an extremely high frequency band component. The major characteristics of the space segment, the ground control station and the portable, aeronautical and mobile user terminals are outlined.

Losquadro, G.↗
NASA NTRS1999

Hypogravity's Effect on the Life Cycle of Japanese Quail

A series of studies were conducted to determine the effect of activities preceding space-flight and during space-flight on quail embryonic development. While the overall development of the quail embryos was evaluated, the report presented herein, focused on calcium utilization or uptake from eggshells by developing embryos during incubation in space and on earth. In the pre-space trials, fertilized quail eggs were subjected to pre-night dynamics including forces of centrifugation, vibration, or a combination of vibration and centrifugation prior to incubation for 6 or 16 days. In another trial, fertile quail eggs were tested for survivability in a refrigerator stowage kit for eggs (RSKE) which was subsequently used to transport the eggs to space. Eggs in the RSKE were subjected to shuttle launch dynamics including G force and random vibration profiles. In the space- flight trials, 48 fertile quail eggs were launched on space shuttle Flight STS-76 and were subsequently incubated in a Slovakian incubator onboard space station, MIR. Two sets of ground controls each with 48 fertile eggs with and without exposure to launch dynamics were initiated 5 days post-launch. There was a laboratory control (incubated in Lyon RX2 incubator at 37.5 C) and a synchronous control (incubated in Lyon RX2 incubator at 39 - 400 C), which simulated the temperature of the space-flight incubator. Following space-flight trials, post-flight trials were conducted where quail eggs were incubated in Lyon RX2 or Slovakian incubators under various temperatures with or without launch dynamics. Eggshells from all study trials were retrieved and analyzed for calcium content to determine if its utilization by developing quail embryos was affected by activities preceding space-flight or during incubation in space under microgravity. Results from the pre-flight and post-flight showed that pre-flight activities and shuttle launch dynamics had no effect on calcium uptake from the eggshell by developing embryos. However, calcium uptake from the eggshell by developing embryos incubated in micro,aravity was impaired by 12.6% when compared to embryos incubated on earth under laboratory control environment. This impairment was unlikely due to factors other than microgravity. In general, calcium utilization by developing embryos increased with age of incubation with the most increase occurring at day 16 of incubation.

Hester, Patricia Y.↗
NASA NTRS1999

Effects of Space Flight, Clinorotation, and Centrifugation on the Growth and Metabolism of Escherichia Coli

Previous experiments have shown that space flight stimulates bacterial growth and metabolism. An explanation for these results is proposed, which may eventually lead to improved terrestrial pharmaceutical production efficiency. It is hypothesized that inertial acceleration affects bacterial growth and metabolism by altering the transport phenomena in the cells external fluid environment. It is believed that this occurs indirectly through changes in the sedimentation rate acting on the bacteria and buoyancy-driven convection acting on their excreted by-products. Experiments over a broad range of accelerations consistently supported this theory. Experiments at I g indicated that higher concentrations of excreted by products surrounding bacterial cells result in a shorter lag phase. Nineteen additional experiments simulated 0 g and 0.5 g using a clinostat, and achieved 50 g, 180 g, and 400 g using a centrifuge. These experiments showed that final cell density is inversely related to the level of acceleration. The experiments also consistently showed that acceleration affects the length of the lag phase in a non-monotonic, yet predictable, manner. Additional data indicated that E. coli metabolize glucose less efficiently at hypergravity, and more efficiently at hypogravity. A space-flight experiment was also performed. Samples on orbit had a statistically significant higher final cell density and more efficient metabolism than did ground controls. These results. which were similar to simulations of 0 g using a clinostat, support the theory that gravity only affects bacterial growth and metabolism indirectly, through changes in the bacteria's fluid environment.

Brown, Robert B.↗
NASA NTRS2000

Development of Sensory Receptors in Skeletal Muscle

The two major goals for this project is to (1) examine the hindlimb walking pattern of offspring from the Flight dams as compared with offspring of the ground control groups from initiation of walking up to two months thereafter; and (2) examine skeletal muscle.

DeSantis, Mark↗