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At least 649 records · Page 36

Data for "Efficient Mutagenesis and Genotyping of Maize Inbreds Using Biolistics, Multiplex CRISPR/Cas9 Editing, and Indel-Selective PCR"

CRISPR/Cas9 based genome editing has advanced our understanding of a myriad of important biological phenomena. Important challenges to multiplex genome editing in maize include assembly of large complex DNA constructs, few genotypes with efficient transformation systems, and costly/labor-intensive genotyping methods. Here we present an approach for multiplex CRISPR/Cas9 genome editing system that delivers a single compact DNA construct via biolistics to Type I embryogenic calli, followed by a novel efficient genotyping assay to identify desirable editing outcomes. We first demonstrate the creation of heritable mutations at multiple target sites within the same gene. Next, we successfully created individual and stacked mutations for multiple members of a gene family. Genome sequencing found off-target mutations are rare. Multiplex genome editing was achieved for both the highly transformable inbred line H99 and Illinois Low Protein1 (ILP1), a genotype where transformation has not previously been reported. In addition to screening transformation events for deletion alleles by PCR, we also designed PCR assays that selectively amplify deletion or insertion of a single nucleotide, the most common outcome from DNA repair of CRISPR/Cas9 breaks by non-homologous end-joining. The Indel-Selective PCR (IS-PCR) method enabled rapid tracking of multiple edited alleles in progeny populations. The ‘end to end’ pipeline presented here for multiplexed CRISPR/Cas9 mutagenesis can be applied to accelerate maize functional genomics in a broader diversity of genetic backgrounds.

gene editing↗

High-Resolution PM2.5 Concentrations Estimation Based on Stacked Ensemble Learning Model Using Multi-Source Satellite TOA Data

Nepal has experienced severe fine particulate matter (PM2.5) pollution in recent years. However, few studies have focused on the distribution of PM2.5 and its variations in Nepal. Although many researchers have developed PM2.5 estimation models, these models have mainly focused on the kilometer scale, which cannot provide accurate spatial distribution of PM2.5 pollution. Based on Gaofen-1/6 and Landsat-8/9 satellite data, we developed a stacked ensemble learning model (named XGBLL) combined with meteorological data, ground PM2.5 concentrations, ground elevation, and population data. The model includes two layers: a XGBoost and Light GBM model in the first layer, and a linear regression model in the second layer. The accuracy of XGBLL model is better than that of a single model, and the fusion of multi-source satellite remote sensing data effectively improves the spatial coverage of PM2.5 concentrations. Besides, the spatial distribution of the daily mean PM2.5 concentrations in the Kathmandu region under different air conditions was analyzed. The validation results showed that the monthly averaged dataset was accurate (R2 = 0.80 and root mean square error = 7.07). In addition, compared to previous satellite PM2.5 datasets in Nepal, the dataset produced in this study achieved superior accuracy and spatial resolution.

Environmental Sciences & Ecology↗

The Highest L(sub X)/L(sub opt) Sources in the ROSAT All-Sky Survey

The purpose of our program was to identify new and interesting soft X-ray sources from the Bright Source Catalog of the ROSAT All-Sky Survey. Our intent was to use XMM to observe a sample of BSC objects that had been identified as less than 10% likely to be associated with any object in the USNO-A2.0 catalog (Rutledge et al. 2000). We requested a single 5-ks pointing for each of 32 sources in this category in order to make a systematic examination of the properties of these sources. Ultimately, we hoped to identify new isolated neutron stars from within this population. Our requested observations were scheduled for execution at the end of Cycle 2, for two of our targets only (selected by sky position). A two-target sample does not allow for the statistical investigation that we originally proposed; however, based on the identification of the BSC object in the XMM data, an improved position, and reevaluation of likely off-band counterparts, it does enable a source-by-source evaluation of whether the BSC object is an isolated neutron star (INS).

Kulkarni, Shrinivas R.↗

Ceramic Inclusions in Powder Metallurgy Disk Alloys: Characterization and Modeling

Powder metallurgy alloys are increasingly used in gas turbine engines, especially in turbine disk applications. Although powder metallurgy materials have many advantages over conventionally cast and wrought alloys (higher strength, higher temperature capability, etc.), they suffer from the rare occurrence of ceramic defects (inclusions) that are inherent to the powder atomization process. These inclusions can have a potentially large detrimental effect on the durability of individual components. An inclusion in a high stress location can act as a site for premature crack initiation and thereby considerably reduce the fatigue life. Because these inclusions are exceedingly rare, they typically do not reveal themselves in the process of characterizing the material for a particular application (the cumulative volume of the test bars in a fatigue life characterization is typically on the order of a single actual component). Ceramic inclusions have, however, been found to be the root cause of a number of catastrophic engine failures. To investigate the effect of these inclusions in detail, we have undertaken a study where known populations of ceramic particles, whose composition and morphology are designed to mimic the "natural" inclusions, are added to the precursor powder. Surface-connected inclusions have been found to have a particularly large detrimental effect on fatigue life; therefore, the quantity of ceramic "seeds" added is calculated to ensure that a minimum number will intersect the surface of the fatigue test bars. Because the ceramic inclusions are irregularly shaped and have a tendency to break up in the process of extrusion and forging, a method of calculating the probability of occurrence and expected intercepted surface area was needed. We have developed a Monte Carlo simulation to determine the distributions of these parameters and have verified the simulated results with observations of ceramic inclusions found in macroscopic slices from extrusions and forgings. Fatigue specimens have been machined from Udimet 720 (a powder metallurgy superalloy) forgings, to determine the effects of the inclusions on fatigue life. The ultimate goal of this study will be to use probabilistic methods to determine the reliability detriment that can be attributed to these ceramic inclusions. This work has been supported by the Ultra Safe and Ultra- Efficient Engine Technologies programs.

Bonacuse, Peter J.↗

The Transitional Protoplanetary Disk Frequency as a Function of Age: Disk Evolution in the Coronet Cluster, Taurus, and Other 1--8 Myr-old Regions

We present Spitzer 3.6-24 micron photometry and spectroscopy for stars in the 1-3 Myr-old Coronet Cluster, expanding upon the survey of Sicilia-Aguilar et al. (2008). Using sophisticated radiative transfer models, we analyze these new data and those from Sicilia-Aguilar et al. (2008) to identify disks with evidence for substantial dust evolution consistent with disk clearing: transitional disks. We then analyze data in Taurus and others young clusters - IC 348, NGC 2362, and eta Cha -- to constrain the transitional disk frequency as a function of time. Our analysis confirms previous results finding evidence for two types of transitional disks -- those with inner holes and those that are homologously depleted. The percentage of disks in the transitional phase increases from approx.15-20% at 1-2 Myr to > 50% at 5-8 Myr; the mean transitional disk lifetime is closer to approx. 1 Myr than 0.1-0.5 Myr, consistent with previous studies by Currie et al. (2009) and Sicilia-Aguilar et al. (2009). In the Coronet Cluster and IC 348, transitional disks are more numerous for very low-mass M3--M6 stars than for more massive K5-M2 stars, while Taurus lacks a strong spectral type-dependent frequency. Assuming standard values for the gas-to-dust ratio and other disk properties, the lower limit for the masses of optically-thick primordial disks is Mdisk approx. 0.001-0.003 M*. We find that single color-color diagrams do not by themselves uniquely identify transitional disks or primordial disks. Full SED modeling is required to accurately assess disk evolution for individual sources and inform statistical estimates of the transitional disk population in large samples using mid-IR colors.

Currie, Thayne↗

Limitations to Biomass Estimation for FAV Using NDVI Analysis of Satellite Acquisitions

The distribution and standing biomass (mass per unit area) of Floating Aquatic Vegetation (FAV)provide assessment metrics for productivity of vegetative communities. Remote sensing acquisition data has been used to estimate standing biomass in various ecosystems using vegetation indices, particularly the normalized difference vegetation index (NDVI), comparing the relative strength of radiation absorbance and reflectance of vegetation in specific wavebands. Obstacles to estimating vegetation biomass using NDVI reported are related to diversity of the vegetation community and high-density saturation. We evaluate the potential for using NDVI derived estimates for FAV biomass in the California Delta by focusing on patches of predominantly single species and compare biomass estimates for specific patches with measurement of total biomass and partitioning within the vegetation canopy. Using Landsat satellite acquisitions, biomass estimates are made for individual pixels and vegetation samples were collected from those same pixels. Focus on patches of predominantly single FAV species resulted in successful estimation of standing biomass beyond that previously reported in other ecosystems. A saturation response for the estimations was observed, however, with a much higher threshold high than previously reported for diverse population aquatic ecosystems. We discuss the likely cause for the saturation phenomenon and potential improvement utilizing altered spectral indices possible with higher spectral resolution from satellites carrying higher waveband multispectral and hyperspectral instruments.

Biomass↗

Shoot growth in aseptically cultivated daylily and haplopappus plantlets after a 5-day spaceflight

Plantlets of daylily (Hemerocallis cv. Autumn Blaze) regenerated from cell suspensions, and 4 clonal populations of Haplopappus gracilis were aseptically cultivated aboard the Shuttle "Discovery" during a 5-day mission within NASA's Plant Growth Unit (PGU) apparatus. Daylily was selected as a representative herbaceous perennial monocotyledon and the haplopappus clones represented an annual dicotyledon. The latter included 4 strains with different physiological and morphological characteristics: two aseptic seedling clones (each generated from a single seedling) and two tissue culture-derived lines. Mean daily growth rates for the primary shoots of all plantlets averaged 4.13 mm day-1 (SD = 2.20) for the flight experiment and 4.68 mm day-1 (SD = 2.59) for the ground control. Comparable growth rates calculated by summing both the primary and secondary shoots for all plantlets were 5.94 mm day-1 (SD = 2.89) for the flight experiment and 6.38 mm day-1 (SD = 3.71) for the control. Statistically significant differences existed between: (1) flight vs control primary shoot growth (the controls growing more than plantlets subjected to spaceflight conditions), (2) the different populations (the daylily gaining more shoot material than any of the haplopappus populations and the haplopappus seedling clones outperforming the tissue culture-derived haplopappus lines), and (3) the individual Plant Growth Chambers contained within the PGU. The data suggest that some spaceflight-associated factor(s) increased the tendency for primary shoot apices to degrade or senesce, resulting in the release of apical dominance and permitting the emergence of axillary branches, which subsequently partially compensated for the reduced primary axis growth. In addition to spaceflight-associated factors, the physiologically diverse nature of the experimental material as well as environmental heterogeneities within the culture apparatus contributed to the variation in growth results. The findings could explain some discrepancies reported from various plant culture experiments conducted in space.

short duration↗

Identification and mapping of quantitative trait loci for Fusarium head blight resistance in a synthetic hexaploid × hard red spring wheat population

Abstract Fusarium head blight (FHB), caused byFusarium graminearumSchwabe, is one of the most devastating diseases in wheat (Triticum aestivumL.). The synthetic hexaploid wheat line Largo was developed from a cross between the durum wheat [T. turgidumssp.durum(Desf.) Husn.] variety Langdon and theAegilops tauschiiCosson accession PI 268210, and it was previously found to have a moderate level of FHB resistance. This study was conducted to identify quantitative trait loci (QTL) associated with FHB resistance using a population of 188 recombinant inbred lines (RILs) from a cross between Largo and the susceptible wheat line ND495. The RILs were evaluated for Type II resistance in two greenhouse and two field environments. The disease severity and 90K single‐nucleotide polymorphism marker data were used for QTL analysis, which revealed six QTL on chromosomes 1D, 2D, 5B, and 7D. Four QTL (QFhb.rwg‐1D,QFhb.rwg‐5B,QFhb.rwg‐7D.1, andQFhb.rwg‐7D.3) from Largo had minor effects, whereas two QTL (QFhb.rwg‐2DandQFhb.rwg‐7D.2) from ND495 showed large effects on FHB resistance. The result suggested that ND495 may possess suppressor or susceptibility gene(s) suppressing or masking FHB resistance controlled by the resistance QTL. Among these QTL, four coincided with previously reported QTL, includingFhb9, and two (QFhb.rwg‐1DandQFhb.rwg‐7D.1) are likely novel QTL. From the six QTL regions, 10 Kompetitive allele‐specific PCR markers were developed and validated for marker‐assisted selection. The QTL detected from the resistant and susceptible parents enhance our understanding of FHB resistance expression and provide new resources for improving FHB resistance in wheat.

Genetics & Heredity↗

Photoluminescence Dynamics Defined by Exciton Trapping Potential of Coupled Defect States in DNA-Functionalized Carbon Nanotubes

Chemical reactions between semiconducting single-wall carbon nanotubes (SWCNTs) and single-stranded DNA (ssDNA) achieve spatially patterned covalent functionalization sites and create coupled fluorescent quantum defects on the nanotube surface, tailoring SWCNT photophysics for applications such as single-photon emitters in quantum information technologies. The evaluation of relaxation dynamics of photoluminescence (PL) from those coupled quantum defects is essential for understanding the nanotube electronic structure and beneficial to the design of quantum light emitters. Here, we measured the PL decay for ssDNA-functionalized SWCNTs as a function of the guanine content of the ssDNA oligo that dictates the red-shifting of their PL emission peaks relative to the band-edge exciton. We then correlate the observed dependence of PL decay dynamics on energy red-shifts to the exciton potential energy landscape, which is modeled using first-principles approaches based upon the morphology of ssDNA-altered SWCNTs obtained by atomic force microscopy (AFM) imaging. In this work, our simulations illustrate that the multiple guanine defects introduced within a single ssDNA strand strongly interact to create a deep exciton trapping well, acting as a single hybrid trap. The emission decay from the distinctive trapping potential landscape is found to be biexponential for ssDNA-modified SWCNTs. We attributed the fast time component of the biexponential PL decay to the redistribution of exciton population among the lowest energy bright states and a manifold of dark states emerging from the coupling of multiple guanine defects. The long lifetime component in the biexponential decay, on the other hand, is attributed to the redistribution of exciton population among different exciton trapping sites that arise from the binding of multiple ssDNA strands along the nanotube axis. AFM measurements indicate that those trapping sites are separated on average by ~8 nm along the nanotube axis.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Red edge spectral measurements from sugar maple leaves

Many sugar maple stands in the northeastern United States experienced extensive insect damage during the 1988 growing season. Chlorophyll data and high spectral resolution spectrometer laboratory reflectance data were acquired for multiple collections of single detached sugar maple leaves variously affected by the insect over the 1988 growing season. Reflectance data indicated consistent and diagnostic differences in the red edge portion (680-750 nm) of the spectrum among the various samples and populations of leaves. These included differences in the red edge inflection point (REIP), a ratio of reflectance at 740-720 nm (RE3/RE2), and a ratio of first derivative values at 715-705 nm (D715/D705). All three red edge parameters were highly correlated with variation in total chlorophyll content. Other spectral measures, including the Normalized Difference Vegetation Index (NDVI) and the Simple Vegetation Index Ratio (VI), also varied among populations and over the growing season, but did not correlate well with total chlorophyll content. Leaf stacking studies on light and dark backgrounds indicated REIP, RE3/RE2 and D715/D705 to be much less influenced by differences in green leaf biomass and background condition than either NDVI or VI.

Vogelmann, J. E.↗

Ceramic Inclusions In Powder Metallurgy Disk Alloys: Characterization and Modeling

Powder metallurgy alloys are increasingly used in gas turbine engines, especially as the material chosen for turbine disks. Although powder metallurgy materials have many advantages over conventionally cast and wrought alloys (higher strength, higher temperature capability, etc.), they suffer from the rare occurrence of ceramic defects (inclusions) that arise from the powder atomization process. These inclusions can have potentially large detrimental effect on the durability of individual components. An inclusion in a high stress location can act as a site for premature crack initiation and thereby considerably reduce the fatigue life. Because these inclusions are exceedingly rare, they usually don't reveal themselves in the process of characterizing the material for a particular application (the cumulative volume of the test bars in a fatigue life characterization is typically on the order of a single actual component). Ceramic inclusions have, however, been found to be the root cause of a number of catastrophic engine failures. To investigate the effect of these inclusions in detail, we have undertaken a study where a known population of ceramic particles, whose composition and morphology are designed to mimic the 'natural' inclusions, are added to the precursor powder. Surface connected inclusions have been found to have a particularly large detrimental effect on fatigue life, therefore the volume of ceramic 'seeds' added is calculated to ensure that a minimum number will occur on the surface of the fatigue test bars. Because the ceramic inclusions are irregularly shaped and have a tendency to break up in the process of extrusion and forging, a method of calculating the probability of occurrence and expected intercepted surface and embedded cross-sectional areas were needed. We have developed a Monte Carlo simulation to determine the distributions of these parameters and have verified the simulated results with observations of ceramic inclusions found in macro slices from extrusions and forgings. The ultimate goal of this study will be to use probabilistic methods to determine the reliability detriment that can be attributed to these ceramic inclusions.

Bonacuse, Pete↗

Unification of X-ray Winds in Seyfert Galaxies: From Ultra-fast Outflows to Warm Absorbers

The existence of ionized X-ray absorbing layers of gas along the line of sight to the nuclei of Seyfert galaxies is a well established observational fact. This material is systematically outflowing and shows a large range in parameters. However, its actual nature and dynamics are still not clear. In order to gain insights into these important issues we performed a literature search for papers reporting the parameters of the soft X-ray warm absorbers (WAs) in 35 type 1 Seyferts and compared their properties to those of the ultra-fast outflows (UFOs) detected in the same sample. The fraction of sources with WAs is >60 per cent, consistent with previous studies. The fraction of sources with UFOs is >34 per cent, >67 per cent of which also show WAs. The large dynamic range obtained when considering all the absorbers together, spanning several orders of magnitude in ionization, column, velocity and distance allows us, for the first time, to investigate general relations among them. In particular, we find significant correlations indicating that the closer the absorber is to the central black hole, the higher the ionization, column, outflow velocity and consequently the mechanical power. In all the cases, the absorbers continuously populate the whole parameter space, with the WAs and the UFOs lying always at the two ends of the distribution. These evidence strongly suggest that these absorbers, often considered of different types, could actually represent parts of a single large-scale stratified outflow observed at different locations from the black hole. The UFOs are likely launched from the inner accretion disc and the WAs at larger distances, such as the outer disc and/or torus. We argue that the observed parameters and correlations are, to date, consistent with both radiation pressure through Compton scattering and magnetohydrodynamic processes contributing to the outflow acceleration, the latter playing a major role. Most of the absorbers, especially the UFOs, show a sufficiently high mechanical power (at least approx 0.5 per cent of the bolometric luminosity) to provide a significant contribution to active galactic nuclei (AGN) feedback and thus to the evolution of the host galaxy. In this regard, we find possible evidence for the interaction of the AGN wind with the surrounding environment on large scales.

Unification↗

Type-Ia Supernova Rates to Redshift 2.4 from Clash: The Cluster Lensing and Supernova Survey with Hubble

We present the supernova (SN) sample and Type-Ia SN (SN Ia) rates from the Cluster Lensing And Supernova survey with Hubble (CLASH). Using the Advanced Camera for Surveys and the Wide Field Camera 3 on the Hubble Space Telescope (HST), we have imaged 25 galaxy-cluster fields and parallel fields of non-cluster galaxies. We report a sample of 27 SNe discovered in the parallel fields. Of these SNe, approximately 13 are classified as SN Ia candidates, including four SN Ia candidates at redshifts z greater than 1.2.We measure volumetric SN Ia rates to redshift 1.8 and add the first upper limit on the SN Ia rate in the range z greater than 1.8 and less than 2.4. The results are consistent with the rates measured by the HST/ GOODS and Subaru Deep Field SN surveys.We model these results together with previous measurements at z less than 1 from the literature. The best-fitting SN Ia delay-time distribution (DTD; the distribution of times that elapse between a short burst of star formation and subsequent SN Ia explosions) is a power law with an index of 1.00 (+0.06(0.09))/(-0.06(0.10)) (statistical) (+0.12/−0.08) (systematic), where the statistical uncertainty is a result of the 68% and 95% (in parentheses) statistical uncertainties reported for the various SN Ia rates (from this work and from the literature), and the systematic uncertainty reflects the range of possible cosmic star-formation histories. We also test DTD models produced by an assortment of published binary population synthesis (BPS) simulations. The shapes of all BPS double-degenerate DTDs are consistent with the volumetric SN Ia measurements, when the DTD models are scaled up by factors of 3-9. In contrast, all BPS single-degenerate DTDs are ruled out by the measurements at greater than 99% significance level.

SUPERNOVA↗

Air Pollution Scenario over Pakistan: Characterization and Ranking of Extremely Polluted Cities using Long-Term Concentrations of Aerosols and Trace Gases

Pakistan ranks third in the world in terms of mortality attributable to air pollution, with aerosol mass concentrations (PM2.5) consistently well above WHO (World Health Organization) air quality guidelines (AQG). However, regulation is dependent on a sparse network of air quality monitoring stations and insufficient ground data. This study utilizes long-term observations of aerosols and trace gases to characterize and rank the air pollution scenarios and pollution characteristics of 80 selected cities in Pakistan. Datasets used include (1) the Aqua and Terra (AquaTerra) MODIS (Moderate Resolution Imaging Spectroradiometer) Level 2 Collection 6.1 merged Dark Target and Deep Blue (DTB) aerosol optical depth (AOD) retrieval products; (2) the CAMS (Copernicus Atmosphere Monitoring Service) reanalysis PM1, PM2.5, and PM10 data; (3) the MERRA-2 (Modern-Era Retrospective analysis for Research and Applications, Version 2) reanalysis PM2.5 data, (4) the OMI (Ozone Monitoring Instrument) tropospheric vertical column density (TVCD) of nitrogen dioxide (NO2), and VCD of sulfur dioxide (SO2) in the Planetary Boundary Layer (PBL), (5) the VIIRS (Visible Infrared Imaging Radiometer Suite) Nighttime Lights data, (6) MODIS Collection 6 Version 2 global monthly fire location data (MCD14ML), (7) population density, (8) MODIS Level 3 Collection 6 land cover types, (9) AERONET (AErosol RObotic NETwork) Version 3 Level 2.0 data, and (10) ground-based PM2.5 concentrations from air quality monitoring stations. Potential Source Contribution Function (PSCF) analyses were performed by integrating with ground-based PM2.5 concentrations and the NOAA (National Oceanic and Atmospheric Administration) HYSPLIT (Hybrid Single-Particle Lagrangian Integrated Trajectory) air parcel back trajectories to identify potential pollution source areas which are responsible for extreme air pollution in Pakistan. Results show that the ranking of the top polluted cities depends on the type of pollutant considered and the metric used. For example, Jhang, Multan, and Vehari were characterized as the top three polluted cities in Pakistan when considering AquaTerra DTB AOD products; for PM1, PM2.5, and PM10 Lahore, Gujranwala, and Okara were the top three; for tropospheric NO2 VCD Lahore, Rawalpindi, and Islamabad and for PBL SO2 VCD Lahore, Mirpur, and Gujranwala. The results demonstrate that Pakistan’s entire population has been exposed to high PM2.5 concentrations for many years, with a mean annual value of 54.7 μg/cu. m, over all Pakistan from 2003 to 2020. This value exceeds Pakistan’s National Environmental Quality Standards (Pak-NEQS, i.e., <15 μg/cu. m annual mean) for ambient air defined by the Pakistan Environmental Protection Agency (Pak-EPA) as well as the WHO Interim Target-1 (i.e., mean annual PM2.5 <35 μg/cu. m). The spatial analyses of the concentrations of aerosols and trace gases in terms of population density, nighttime lights, land cover types, and fire location data, and the PSCF analysis indicate that Pakistan’s air quality is strongly affected by anthropogenic sources inside of Pakistan, with contributions from surrounding countries. Statistically significant positive (increasing) trends in PM1, PM2.5, PM10, tropospheric NO2 VCD, and SO2 VCD were observed in ~89%, ~67%, ~48%, 91%, and ~88% of the Pakistani cities (80 cities), respectively. This comprehensive analysis of aerosol and trace gas levels, their characteristics in spatio-temporal domains, and their trends over Pakistan, is the first of its kind. Results will be helpful to the Ministry of Climate Change (Government of Pakistan), Pak-EPA, SUPARCO (Pakistan Space and Upper Atmosphere Research Commission), policymakers, and the local research community to mitigate air pollution and its effects on human health.

Muhammad Bilal↗

A UNIONS view of the brightest central galaxies of candidate fossil groups

The formation process of fossil groups (FGs) is still under debate, and, because of their relative rarity, large samples of such objects are still missing. The aim of the present paper is to increase the sample of known FGs, to analyse the properties of their brightest group galaxies (BGGs), and to compare them with a control sample of non-FG BGGs. We extracted a sample of 87 FG and 100 non-FG candidates from a large spectroscopic catalogue of haloes and galaxies. For all the objects with data available in UNIONS (initially the Canada France Imaging Survey, CFIS) in the u and r bands, and/or in an extra r-band processed to preserve all low-surface-brightness features (rLSB), we performed a 2D photometric fit of the BGG with GALFIT with one or two Sérsic components. We also analysed how the subtraction of the intracluster light (ICL) contribution modifies the BGG properties. From the SDSS spectra available for the BGGs of 65 FGs and 82 non-FGs, we extracted the properties of their stellar populations with Firefly. To complement our study, and in order to provide a detailed illustration of the possible origin of emission lines in the FG BGGs, involving the presence or absence of an AGN, we investigated the origin of the emission lines in a nearby FG that is dominated by the NGC 4104 galaxy. Morphologically, a single Sérsic profile can fit most objects in the u band, while two Sérsics are needed in the r and rLSB bands, both for FGs and non-FGs. Non-FG BGGs cover a larger range of Sérsic index n. FG BGGs follow the Kormendy relation (mean surface brightness versus effective radius) previously derived for almost 1000 brightest cluster galaxies (BCGs), while the majority of non-FGs BGGs are located below this relation, with fainter mean surface brightnesses. This suggests that FG BGGs have evolved similarly to BCGs, and non-FG BGGs have evolved differently from both FG BGGs and BCGs. All the above properties can be strongly modified by the subtraction of the ICL contribution. Based on spectral fitting, the stellar populations of FG and non-FG BGGs do not differ significantly. FG and non-FG BGGs differ from one another in terms of their morphological properties and Kormendy relation, suggesting they have had different formation histories. However, it is not possible to trace differences in their stellar populations or in their large-scale distributions.

79 ASTRONOMY AND ASTROPHYSICS↗

Cross-cutting strategies to lower electricity use of miscellaneous electric loads in the domestic sector

Miscellaneous Electric Loads (MELs) account for roughly one quarter of building electricity use in most developed countries. A product-specific approach to lowering MELs electricity use in this category takes too long and costs too much because there are so many MELs, each providing unique services. An alternative approach focusing on key functionalities was therefore explored. These functionalities include: (1) power management, (2) power scaling, and (3) power conversion. Cross-cutting efficiency improvements to these functionalities can be incorporated into broad categories of MELs, thus saving electricity and lowering costs. Even though the population of MELs is diverse and rapidly evolving, major technical opportunities exist to improve their efficiency in these functionalities. Research into energy-saving solutions within the cross-cutting technologies will probably have larger savings than focusing on single products.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

Radiative dynamics and delayed emission in pure and doped InP/ZnSe/ZnS quantum dots

We have used time-correlated single photon counting to elucidate the radiative dynamics of InP/ZnSe/ZnS core/shell/shell quantum dots (QDs) that differ in the amount and distribution of excess indium. Stoichiometric QDs having an In:P atom ratio very near unity exhibit simple luminescence kinetics. The photoluminescence (PL) rises with the 40 ps instrument response function and exhibits a decay that is close to a single exponential with a time constant that decreases from 32 to 28 ns with increasing shell thickness. QDs having excess indium (In:P ratio of 1.15–1.63) show a significant component of a slower rise time assigned to transient population of indium-based hole traps in the ZnSe shell. They also have a slower PL decay, attributed to an equilibrium between these traps, which are optically dark, and the emissive valence-band state. This results in a radiative lifetime that increases from 32 to 48 ns with increasing shell thickness. Different treatments of the InP cores prior to shell deposition result in different core/shell interfaces as indicated by resonance Raman spectroscopy, as well as differences in the amplitude and timescale of the slow PL rise and the PL decay time. These are interpreted in terms of different radial distributions of the indium-based hole traps, which can be related to differences in the interfacial lattice strain.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗