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NASA NTRSDate not supplied

Transcriptomic Changes in Peripheral Blood Mononuclear Cells of International Space Station Crewmembers

In space, living organisms are exposed to numerous stress factors including microgravity and space radiation. For humans, these harmful environmental factors have been known to cause negative health impacts such as immune dysfunction. Understanding the mechanisms by which spaceflight impacts human health at the molecular level is critical not only for accurately assessing the risks associated with spaceflight, but also for developing effective countermeasures. This study is part of the Functional Immune Project, intended to determine alterations in crewmembers` immunobiology before, during, and after spaceflight. For this project, blood samples were collected from International Space Station (ISS) crewmembers at the following time points: i) Blood was drawn at two pre-flight time points of 180 days (L180) and 45 days (L45) before launch. ii) During flight, blood was drawn at approximately the midpoint (mid-flight, MF) of the mission, and shortly before egress from the ISS (late-flight, LF). iii) Post-flight blood samples were collected within 36 hours (R0), 30 days (R30) and 90 days (R90) after landing. For each crewmember, blood was also drawn from a matching test subject on the ground at the corresponding time point. For both the ISS crewmembers and the ground control subjects, total RNA was isolated from peripheral blood mononuclear cells (PBMC) and mRNA was analysed using next generation RNA-sequencing (NGS). Differentially expressed genes were determined by performing contrast analysis. Using the ground control subjects of all time points combined as a control, a number of dysregulated genes were identified in astronauts at MF, LF and R0, including downregulations of SMAD7 and CDKN1A at MF and LF. Some of the genes such as SERPINE1 and VEGFA were downregulated at MF and LF, but upregulated at R0, while others such as NKG7 were down regulated at all 3 time points. Pathway analysis of these differentially expressed genes indicated that the NF-κB pathway was chronically activated in space. Analysis of the consequent diseases suggested potential associations with not only immune dysfunction, but also other health risks including osteoarthritis, cardiac hypertrophy and neuroinflammation.

Maria Moreno-Villanueva↗
NASA NTRSDate not supplied

Gene expression changes in peripheral blood mononuclear cells of ISS crewmembers suggest impacts of spaceflight on cell death

In space, living organisms are exposed to numerous stress factors including microgravity and space radiation. For humans, these harmful environmental factors have been known to cause negative health impacts such as immune dysfunction. Understanding the mechanisms by which spaceflight impacts human health at the molecular level is critical not only for accurately assessing the risks associated with spaceflight, but also for developing effective countermeasures. This study is part of the Functional Immune Project, intended to determine alterations in crewmembers` immunobiology before, during, and after spaceflight. For this project, blood samples were collected from International Space Station (ISS) crewmembers at the following time points: i) at two pre-flight time points of 180 days (L180) and 45 days (L45) before launch. ii) During flight, blood was drawn at approximately the midpoint (mid-flight, MF) of the mission, and shortly before egress from the ISS (late-flight, LF). iii) Post-flight blood samples were collected within 24 hrs (R0), 30 days (R30) and 90 days (R90) after landing. For each crewmember, blood was also drawn from a matching test subject on the ground at the corresponding time point. For both the ISS crewmembers and the ground control subjects, total RNA was isolated from peripheral blood mononuclear cells (PBMC) and mRNA was analysed using next generation RNA-sequencing (NGS). Differentially expressed genes were determined by performing contrast analysis. Using the data from all of the time points from the ground control subjects as a control, a number of dysregulated genes were identified in astronauts at MF, LF and R0, including downregulations of several cell cycle related genes including CDKN1A and VEGFA at MF and LF. Pathway analysis of these differentially expressed genes indicated that, in space, pathways associated with autophagy and senescence were affected. Our analysis also indicated that the genes related to metabolisms were downregulated in the microgravity environment. Taken together, our data suggests that PBMC in the ISS crewmembers may be starved, resulting in autophagy and delayed senescence in space. Such findings are in agreement with delayed cell death in PBMC under simulated microgravity conditions on the ground and offer an explanation for telomere lengthening that has been reported among the ISS astronauts in flight.

Maria Moreno-Villanueva↗
NASA NTRSDate not supplied

Transcriptomic Changes in ISS Crewmembers Suggest Decreased Metabolic Activity in Peripheral Blood Mononuclear Cells

In space, living organisms are exposed to numerous stress factors including microgravity and space radiation. For humans, these harmful environmental factors have been known to cause negative health impacts such as immune dysfunction. Understanding the mechanisms by which spaceflight impacts human health at the molecular level is critical not only for accurately assessing the risks associated with spaceflight, but also for developing effective countermeasures. This study is part of the Functional Immune Project, intended to determine alterations in crewmembers` immunobiology before, during, and after spaceflight. For this project, blood samples were collected from International Space Station (ISS) crewmembers at the following time points: i) Blood was drawn at two pre-flight time points of 180 days (L180) and 45 days (L45) before launch. ii) During flight, blood was drawn at approximately the midpoint (mid-flight, MF) of the mission, and shortly before egress from the ISS (late-flight, LF). iii) Post-flight blood samples were collected within 36 hours (R0), 30 days (R30) and 90 days (R90) after landing. For each crewmember, blood was also drawn from a matching test subject on the ground at the corresponding time point. For both the ISS crewmembers and the ground control subjects, total RNA was isolated from peripheral blood mononuclear cells (PBMC) and mRNA was analysed using next generation RNA-sequencing (NGS). Differentially expressed genes were determined by performing contrast analysis. Using the ground control subjects of all time points combined as a control, a number of dysregulated genes were identified in astronauts at MF, LF and R0, Pathway analysis of these differentially expressed genes indicated that several of the pathways related to metabolism, including LXR/RXR, NAD signaling and fatty acid betta-oxidation, were downregulated at MF and LF. We suggest that the decreased metabolic activity in space may contribute to the immune dysfunction and delayed cell cycle progression as observed in the astronauts.

Maria Moreno-Villanueva↗
NASA NTRSDate not supplied

Gene Expression Changes in Peripheral Blood Mononuclear Cells of ISS Crewmembers Suggest Impacts of Spaceflight on Cell Death

In space, living organisms are exposed to numerous stress factors including microgravity and space radiation. For humans, these harmful environmental factors have been known to cause negative health impacts such as immune dysfunction. Understanding the mechanisms by which spaceflight impacts human health at the molecular level is critical not only for accurately assessing the risks associated with spaceflight, but also for developing effective countermeasures. This study is part of the Functional Immune Project, intended to determine alterations in crewmembers` immunobiology before, during, and after spaceflight. For this project, blood samples were collected from International Space Station (ISS) crewmembers at the following time points: i) at two pre-flight time points of 180 days (L180) and 45 days (L45) before launch. ii) During flight, blood was drawn at approximately the midpoint (mid-flight, MF) of the mission, and shortly before egress from the ISS (late-flight, LF). iii) Post-flight blood samples were collected within 24 hrs (R0), 30 days (R30) and 90 days (R90) after landing. For each crewmember, blood was also drawn from a matching test subject on the ground at the corresponding time point. For both the ISS crewmembers and the ground control subjects, total RNA was isolated from peripheral blood mononuclear cells (PBMC) and mRNA was analysed using next generation RNA-sequencing (NGS). Differentially expressed genes were determined by performing contrast analysis. Using the data from all of the time points from the ground control subjects as a control, a number of dysregulated genes were identified in astronauts at MF, LF and R0, including downregulations of several cell cycle related genes including CDKN1A and VEGFA at MF and LF. Pathway analysis of these differentially expressed genes indicated that, in space, pathways associated with autophagy and senescence were affected. Our analysis also indicated that the genes related to metabolisms were downregulated in the microgravity environment. Taken together, we hypothesize that PBMC in the ISS crewmembers may be starved, resulting in autophagy and delayed senescence in space. Such findings are in agreement with delayed cell death in PBMC under simulated microgravity conditions on the ground and offer an explanation for telomere lengthening that has been reported among the ISS astronauts in flight

Maria Moreno-Villanueva↗
NASA NTRS1998

The Postshock Chemical Lifetimes of Outflow Tracers and a Possible New Mechanism to Produce Water Ice Mantles

We have used a coupled time-dependent chemical and dynamical model to investigate the lifetime of the chemical legacy in the wake of C-type shocks. We concentrate this study on the chemistry of H2O and O2, two molecules which are predicted to have abundances that are significantly affected in shock-heated gas. Two models are presented: (1) a three-stage model of preshock, shocked, and postshock gas; and (2) a Monte Carlo cloud simulation where we explore the effects of stochastic shock activity on molecular gas over a cloud lifetime. For both models we separately examine the pure gas-phase chemistry as well as the chemistry including the interactions of molecules with grain surfaces. In agreement with previous studies, we find that shock velocities in excess of 10 km/s are required to convert all of the oxygen not locked in CO into H2O before the gas has an opportunity to cool. For pure gas phase models the lifetime of the high water abundances, or "H2O legacy," in the postshock gas is approximately (4-7) x 10(exp 5) yr, independent of the gas density. A density dependence for the lifetime of H2O is found in gas-grain models as the water molecules deplete onto grains at the depletion timescale. Through the Monte Carlo cloud simulation we demonstrate that the time-average abundance of H2O, the weighted average of the amount of time gas spends in preshock, shock, and postshock stages, is a sensitive function of the frequency of shocks. Thus we predict that the abundance of H2O, and to a lesser extent O2, can be used to trace the history of shock activity in molecular gas. We use previous large-scale surveys of molecular outflows to constrain the frequency of 10 km/s shocks in regions with varying star formation properties and discuss the observations required to test these results. We discuss the postshock lifetimes for other possible outflow tracers (e.g., SiO and CH3OH) and show that the differences between the lifetimes for various tracers can produce potentially observable chemical variations between younger and older outflows. For gas-grain models we find that the abundance of water-ice on grain surfaces can be quite large and is comparable to that observed in molecular clouds. This offers a possible alternative method to create water mantles without resorting to grain surface chemistry: gas heating and chemical modification due to a C-type shock and subsequent depletion of the gas-phase species onto grain mantles.

Bergin, Edwin A.↗
NASA NTRS1999

Effective Forces Between Colloidal Particles

Colloidal suspensions have proven to be excellent model systems for the study of condensed matter and its phase behavior. Many of the properties of colloidal suspensions can be investigated with a systematic variation of the characteristics of the systems and, in addition, the energy, length and time scales associated with them allow for experimental probing of otherwise inaccessible regimes. The latter property also makes colloidal systems vulnerable to external influences such as gravity. Experiments performed in micro-ravity by Chaikin and Russell have been invaluable in extracting the true behavior of the systems without an external field. Weitz and Pusey intend to use mixtures of colloidal particles with additives such as polymers to induce aggregation and form weak, tenuous, highly disordered fractal structures that would be stable in the absence of gravitational forces. When dispersed in a polarizable medium, colloidal particles can ionize, emitting counterions into the solution. The standard interaction potential in these charged colloidal suspensions was first obtained by Derjaguin, Landau, Verwey and Overbeek. The DLVO potential is obtained in the mean-field linearized Poisson-Boltzmann approximation and thus has limited applicability. For more precise calculations, we have used ab initio density functional theory. In our model, colloidal particles are charged hard spheres, the counterions are described by a continuum density field and the solvent is treated as a homogeneous medium with a specified dielectric constant. We calculate the effective forces between charged colloidal particles by integrating over the solvent and counterion degrees of freedom, taking into account the direct interactions between the particles as well as particle-counterion, counterion-counterion Coulomb, counterion entropic and correlation contributions. We obtain the effective interaction potential between charged colloidal particles in different configurations. We evaluate two- and three-body forces in the bulk as well as study the influence of soft walls. We qualitatively explain the effects of the walls on the forces and demonstrate that many-body effects are negligible in our system. With adjustments in the parameters, the DLVO pair-potential can describe the results quantitatively. Besides electrostatic interactions, entropic depletion effects that arise from (hard-core) exclusion play an important role in determining the behavior of multi-component colloidal suspensions. A standard theory for depletion forces is due to Asakura and Oosawa and is based on the ideal gas approximation. To go beyond this approximation, we have studied entropic forces in molecular dynamics simulations of systems of hard spheres (the effects of the solvent have been ignored). The effective depletion forces for these systems can be found either from equilibrium distribution functions or from direct momentum transfer calculations. Our results obtained by either method show qualitative differences from the Asakura-Oosawa forces, indicating a longer range, higher value at contact and most importantly a more complicated structure, comprising of several maxima and minima. Our calculations include the determination of effective forces between two spheres, a hard sphere and a wall, and the behavior of a hard sphere near a step-edge and a corner. We also demonstrate that such entropic forces do not necessarily satisfy pairwise additivity.

Tehver, Riina↗
NASA NTRS2002

Molecular Genetics of Root Thigmoresponsiveness in Arabidopsis thaliana

The molecular mechanisms that allow plant roots to use gravity and touch as growth guides are investigated. We are using a molecular genetic strategy in Arabidopsis thaliana to study these processes. When Arabidopsis thaliana seedlings grow on tilted hard-agar surfaces, their roots develop a wavy pattern of growth which appears to derive from a succession of left-handed and right-handed circumnutation-like processes triggered by gravity and touch stimulation (Okada and Shimura, 1990; Rutherford et al., 1998; Rutherford and Masson, 1996). Interestingly, mutations that affect root waving on tilted hard-agar surfaces can be identified and characterized. Some of these mutations affect root gravitropism, while others appear to be responsible for the production of abnormal waves (no waves, compressed or square waves, coils) without affecting gravitropism. The specific objectives of this project were to functionally characterize two genes (WVD2 and WVD6) which are required for root waving on tilted agar surfaces, but not for root gravitropism. Specific objectives included a physiological and cytological analysis of the mutants, and molecular cloning and characterization of the corresponding genes. As summarized in this paper, we have reached these objectives. We have also identified and partially characterized other mutations that affect root skewing on hard-agar surfaces (sku5-1 and ago1), and have completed our work on the root-wave phenotype associated with mutations in genes of the tryptophan biosynthesis pathway (Lynn et al., 1999; Rutherford et al., 1998; Sedbrook et al., 2000, 2002). We briefly describe our progress on the cloning and characterization of WVD6, WVD2 and SKU5, and provide a list of papers (published, or in preparation) that derived from this grant. We also discuss the biological implications of our findings, with special emphasis on the analysis of WVD2.

Masson, Patrick H.↗
NASA NTRS1972

Functionally terminated liquid nitroso fluorocarbon terpolymers

Properties of polymer for conformal coating for electronic circuitry are described. Nitroso fluorocarbon polymers were selected for application. Chemical reactions for production of polymers are discussed. Technique allows regulation of crosslink densities, molecular weight, and viscosity.

Mayes, N.↗
NASA NTRS1976

A statistical method for treating molecular line opacities

A method for treating atomic and molecular line opacities in cool stellar atmospheres by a statistical opacity sampling is investigated. Under the usual assumptions of plane-parallel geometry, radiative equilibrium, hydrostatic equilibrium, and LTE, each radiative quantity is computed monochromatically at each chosen frequency and depth without any averaging of the opacity. The number of frequencies needed to allow an accurate integration of the energy flux over a given spectral interval is investigated as a function of depth, including opacity for both CN and C2. This method is extended to the calculation of a model atmosphere of a star, and the effect of the number and placement of frequency points is studied. The method is applied to treating molecular lines of CO, C2, and CN in a cool carbon star. Significant advantages of the opacity sampling method are its flexibility, which permits computation of models having arbitrary variations of chemical composition and of opacity with wavelength and depth, and generalizability to include departures from LTE.

Sneden, C.↗
NASA NTRS1987

SAGE aerosol measurements. Volume 3: January 1, 1981 to November 18, 1981

The Stratospheric Aerosol and Gas Experiment (SAGE) satellite system, launched February 18, 1979, obtained profiles of aerosol extinction at 1.00 micron and 0.45 micron ozone concentration, and nitrogen dioxide concentration. Data taken during sunset events are presented in the form of zonal and seasonal averages of aerosol extinction of 1.00 micron and 0.45 micron, ratios of aerosol extinction to molecular extinction at 1.00 micron and ratios of aerosol extinction at 0.45 micron to aerosol extinction at 1.00 micron. Averages for 1981 are shown in tables, and in profile and contour plots (as a function of altitude and latitude). In addition, temperature data provided by NOAA for the time and location of each SAGE measurement are averaged and shown in a similar format. The stratospheric aerosol distribution for 1981 shows effects of volcanically injected material from eruptions of Ulawun, Alaid, and Pagan. Peak values of aerosol extinction at 0.45 micron and 1.00 micron were 2 to 4 times higher than typical peak values observed during near background conditions. Stratospheric aerosol optical depth values at 1.00 microns increased by a factor of about 2 from near background levels in regions of volcanic activity. During the year, these values ranged from between 0.001 and 0.006. The largest were near the location of a recent eruption. The distribution of the ratio of aerosol to molecular extinction at 1.00 microns also showed that maximum values are found in the vicinity of an eruption. These maximums varied in altitude, but remained below a height of about 25 km. No attempt has been made to give detailed explanations or interpretations of these data. The intent is to provide, in a ready-to-use visual format, representative zonal and seasonal averages of aerosol extinction data for the third calendar year of the SAGE data set to facilitate atmospheric and climatic studies.

Mccormick, M. Patrick↗
NASA NTRS1975

Shape of the 5 mm oxygen band in the atmosphere

The problem of absorption of microwaves by molecular oxygen in the atmosphere is treated by means of a first-order approximation to the impact theory of overlapping spectral lines. By including only the coupling between adjacent rotational states in molecular collisions, we have devised a simple approximate method for computing the interference between lines from measurements on the resolved lines. The need for an empirically determined function describing the linewidth/pressure ratio is eliminated. Comparisons with measurements at atmospheric pressures show that the first-order interference accounts for the low absorption at the band wings near 1 atm pressure. It also predicts the correct amount of asymmetry between high and low frequency wings.

Source record↗
NASA NTRS1976

Prediction of the diffuse far infrared flux from the galactic plane

A basic model and simple numerical relations useful for future far infrared studies of the galaxy are presented. Making use of recent CO and other galactic surveys, the diffuse far infrared flux distribution from the galactic plane as a function of galactic longitude alternate theta for 4 deg or = alternate theta or = 90 and the far infrared emissivity as a function of galactocentric distance is predicted. Future measurements of the galactic far infrared flux would yield valuable information on the physical properties and distribution of dust and molecular clouds in the galaxy, particularly the inner region.

Fazio, G. G.↗
NASA NTRS1977

Photoabsorption in formaldehyde

Theoretical studies of the vertical electronic dipole excitation and ionization spectra in molecular formaldehyde are reported. The investigations relied on configuration-interaction calculations and moment-theory techniques. A double-zeta basis of contracted Gaussian-lobe functions supplemented with appropriate polarization and bond functions was used to construct Fock spectra in C(2 nu) symmetry for certain states near the ground state equilibrium geometry. The ionization energies, discrete vertical transition frequencies, and oscillator strengths for occupied and vertical Fock orbitals are in general accord with experimental determinations and other theoretical calculations. Stieltjes and Chebyshev vertical electronic photoionization profiles were calculated and found to be in good agreement with appropriately averaged photoionization-mass spectrometric measurements of the cross section for parent H2CO(+) ion production.

Langhoff, P. W.↗
NASA NTRS1989

New measurements of the far ultraviolet scattering properties of interstellar dust

An analysis is presented for the spectra of the diffuse ultraviolet background taken during shuttle flight STS-61C (January 1986). Eight regions of the sky were observed for approximately 20 minutes each, using a spectrograph designed specifically to perform measurements of the UV background. The field of view was 3.8 deg x 8 min, with imaging along the slit to confine stellar contamination. The instrument featured a shutter mechanism to measure internal background during flight, a low-scatter holographically ruled diffraction grating, photon counting microchannel plate detectors, through baffling, and a crystal window to further attenuate stray light. The spectra covered the range 1400 to 1850 A and was binned in 50 A bands. The procedure for substracting the contribution of stars too faint to be detected as discrete sources during the observations (in general this represents a small fraction of the total intensity detected except at the longest wavelengths). A radiative transfer model used to interpret the data and set confidence intervals on the relevant parameters is described. It was found that the continuum component of the diffuse ultraviolet background arises primarily from two sources. One source is scattering of starlight by interstellar dust with an albedo of about 12 percent and a relatively isotropic phase function. A second source consists of about 150 photons/cm/sec/ster/A of extragalactic light which is attenuated by the dust in the galaxy. Although emission features possibly associated with molecular H2 are detected in one look direction, fluorescence of H2 is not a major contributor to the diffuse UV background, at least at galactic latitudes greater than -10 degrees.

Hurwitz, Mark↗
NASA NTRS1992

SKIRT Space Shuttle glow experiment

This paper describes a spectrometer/radiometer experiment to obtain infrared, visible, and ultraviolet measurements of Space Shuttle glow. The payload, Spacecraft Kinetic Infrared Test (SKIRT), is a cryogenic circular variable filter infrared spectrometer with a number of infrared, visible, and ultraviolet radiometers covering the spectral range of 0.2-5.4 microns and 9.9-10.3 microns. It will measure Shuttle glow as a function of mission elapsed time, orbiter attitude, temperature, and orbiter events such as thruster firings. The measured data should have sufficient spectral resolution and sensitivity to identify molecular species contributing to Shuttle glow emissions. SKIRT is manifested on STS-39.

Ahmadjian, M.↗
NASA NTRS1994

Rapid computation of spectrally integrated non-local thermodynamic equilibrium limb emission

The interpretation of infrared radiance measurements made by satellite-borne limb-scanning broadband radiometers requires accurate and computationally fast techniques with which to evaluate the equation of radiative transfer. This requirement is made even more stringent when analyzing measurements of non-local thermodynamic equilibrium (non-LTE) emission from the terrestrial mesosphere and lower thermosphere. In principle, line-by-line calculations which explicitly account for the departure from thermodynamic equilibrium in both the source functions and the transmittances are necessary. In this paper we extend the emissivity growth approximation (EGA) technique developed for local thermodynamic equilibrium (LTE) limb radiance for the molecular oxygen dayglow (1.27 micrometers and 762 nm), ozone and carbon dioxide in the 9- to 11-micrometer spectral interval, carbon monoxide (4.6 micrometers), nitric oxide (5.3 micrometers), and the carbon dioxide bands (15 micrometers) are presented. Using the non-LTE form of the EGA, the spectrally integrated limb emission is calculated for 35 tangent heights in the mesosphere and lower thermosphere (a total of 1200 atmospheric layers) with line-by-line accuracy in approximately 0.35 s of CPU time on readily available desktop computer hardware, while the corresponding line-by-line calculations may require several minutes. The non-LTE EGA technique will allow kinetic temperature and minor constituend retrieval algorithms to readily include non-LTE efects limited only by the a priori knowledge of the departure from LTE in the observed bands.

Mlynczak, Martin G.↗
NASA NTRS1994

Some Aspects of the Implementation of Double Group Symmetry and Electron Correlation in Molecular 4-Component Calculations

The efficient implementation of method for electron correlation in molecular 4-component calculations demands that symmetry be exploited where possible. Algorithms for the construction of matrices and the transformation of integrals over symmetry-adapted basis functions, where the point group is restricted to D(sub 2h) and subgroups, will be presented. The merits of keeping the primitive integrals in the scalar basis will be compared with those of transforming them to the 2-spinor basis.

Dyall, Kenneth G.↗
NASA NTRS2001

The sugar model: catalytic flow reactor dynamics of pyruvaldehyde synthesis from triose catalyzed by poly-l-lysine contained in a dialyzer

The formation of pyruvaldehyde from triose sugars was catalyzed by poly-l-lysine contained in a small dialyzer with a 100 molecular weight cut off (100 MWCO) suspended in a much larger triose substrate reservoir at pH 5.5 and 40 degrees C. The polylysine confined in the dialyzer functioned as a catalytic flow reactor that constantly brought in triose from the substrate reservoir by diffusion to offset the drop in triose concentration within the reactor caused by its conversion to pyruvaldehyde. The catalytic polylysine solution (400 mM, 0.35 mL) within the dialyzer generated pyruvaldehyde with a synthetic intensity (rate/volume) that was 3400 times greater than that of the triose substrate solution (12 mM, 120 mL) outside the dialyzer. Under the given conditions the final yield of pyruvaldehyde was greater than twice the weight of the polylysine catalyst. During the reaction the polylysine catalyst was poisoned presumably by reaction of its amino groups with aldehyde reactants and products. Similar results were obtained using a dialyzer with a 500 MWCO. The dialyzer method of catalyst containment was selected because it provides a simple and easily manipulated experimental system for studying the dynamics and evolutionary development of confined autocatalytic processes related to the origin of life under anaerobic conditions.

Biogenesis↗