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At least 649 records · Page 36

Ecology and thermal inactivation of microbes in and on interplanetary space vehicle components

Studies were made of atypical organisms found in Bacillus subtilis var. niger spore colonies. Efforts were aimed at: (1) determining the heat sensitivity of these atypical white spores treated under dry heat conditions and their influence on the nature of the survival curve, (2) preparing a new spore crop obtained from spore isolates by purification procedures, and (3) comparing spore crops obtained from Cape Kennedy (SSM-10) and Minnesota (Minn. sp. AAEF) with the old Cincinnati and new purified Cincinnati spore crop under dry heat conditions.

Campbell, J. E.↗

Isolation of Purines and Pyrimidines from the Murchison Meteorite Using Sublimation

The origin of life on Earth, and possibly on other planets such as Mars, would have required the presence of liquid water and a continuous supply of prebiotic organic compounds. The exogenous delivery of organic matter by asteroids, comets, and carbonaceous meteorites could have contributed to the early Earth s prebiotic inventory by seeding the planet with biologically important organic compounds. A wide variety of prebiotic organic compounds have previously been detected in the Murchison CM type carbonaceous chondrite including amino acids, purines and pyrimidines. These compounds dominate terrestrial biochemistry and are integral components of proteins, DNA and RNA. Several purines including adenine, guanine, hypoxanthine, and xanthine, as well as the pyrimidine uracil, have previously been detected in water or formic acid extracts of Murchison using ion-exclusion chromatography and ultraviolet spectroscopy. However, even after purification of these extracts, the accurate identification and quantification of nucleobases is difficult due to interfering UV absorbing compounds. In order to reduce these effects, we have developed an extraction technique using sublimation to isolate purines and pyrimidines from other non-volatile organic compounds in Murchison acid extracts.

Glavin, D. P.↗

A Snapshot of the Continuous Emission of the Active Galactic Nucleus in NGC 3783 from Gamma-Ray to Radio Wavelengths

To better understand the physical processes that produce the continuous emission in active galactic nuclei (AGN), a snapshot of the overall continuous energy distribution of NGC 3783, from gamma ray to radio wavelengths, has been obtained within the framework of the World Astronomy Days. The data collected in this campaign are from GRO, ROSAT, Voyager 2, IUE, HST, CTIO, SAAO, and the VLA. Great care has been taken in disentangling the genuine AGN continusous emission from other contributions; depending on the waveband, the latter might be (1) unrelated contaminating sources in cases where the instrument field of view is large (2) components within which the AGN is embedded, such as the stellar bulge population which accounts for a significant fraction of the optical continuum, and free-bound and FE2 blends wich contribute to the ultraviolet flux. After correction for these other contributins, the continuous emission of the isolated AGN appears to be rather flat (i.e., approximately equal energy per unit logarithmic frequency) from soft gamma ray to infrared wavelengths. At high energies (0.1 MeV to 0.1 keV), the AGN continuum can be fitted by a power law F nu approaches Nu(exp -a) with a spectral index of alpha approximately 1. At longer wavelengths, two excesses above this power law ('bumps') appear: in the ultraviolet, the classical big blue bump, which can be interpreted as thermal emission from the accretion disc surrounding a massive black hole, and in the infrared, a second bump which can be ascribed to thermal emission from dust in the vicinity of the AGN, heated by ultraviolet radiation from the central source. By fitting accretion-disk models to the observed AGN spectral energy distribution, we find values for the accretion disk innermost temperature, accretion rate, and black hole mass, with some differences that depend on whether or not we extrapolate the high energy power law up to infrared wavelengths. A fit to the IR bump above the extended alpha equals 1 power law suggests the presence of a dust component covering the region from a distance rho approximately equals 80 light days (hot grains at a temperature of approximately equals 1500 K) to rho approximately equals 60 light years (cool grains at T approximately equals 200 K). The total mass of dust is around 60 solar masses.

Alloin, D.↗

Computational investigation on the combined effect of pore attributes on strain concentrators in metal additively manufactured materials

Metal additive manufacturing provides a pathway for creating highly optimized components. However, porosity continues to be a prevalent issue for fatigue performance despite best efforts in optimizing process parameters and postprocessing techniques. This work uses finite element analysis to conduct a parametric study to link various pore attributes (i.e., aspect ratio, orientation, and location) to strain concentration factors (SCF) under elastic/plastic deformation during uniaxial loading. Keyhole and lack-of-fusion pores are idealized by prolate and oblate ellipsoids, respectively. Each simulation of the parametric study assumes a single isolated pore in a Ti-6Al-4V material, modeled with J2-plasticity. A reduced-order model is developed to relate pore attributes to SCF, and then used to quantify the variability in SCF for a given experimentally characterized probability distribution of pore attributes. This reduced-order model can be used in a production environment to provide a first-order evaluation to rapidly screen sub-optimally performing components.

Additive Manufacturing↗

Study of design and control of remote manipulators. Part 2: Vibration considerations in manipulator design

An investigation is reported involving a mathematical procedure using 4 x 4 transformation matrices for analyzing the vibrations of flexible manipulators. Previous studies with the procedure are summarized and the method is extended to include flexible joints as well as links, and to account for the effects of various power transmission schemes. A systematic study of the allocation of structural material and the placement of components such as motors and gearboxes was undertaken using the analytical tools developed. As one step in this direction the variables which relate the vibration parameters of the arm to the task and environment of the arm were isolated and nondimensionalized. The 4 x 4 transformation matrices were also used to develop analytical expressions for the terms of the complete 6 x 6 compliance matrix for the case of two flexible links joined by a rotating joint, flexible about its axis of rotation.

Book, W. J.↗

Computational Modeling of Vortex Generators for Turbomachinery

In this work computational models were developed and used to investigate applications of vortex generators (VGs) to turbomachinery. The work was aimed at increasing the efficiency of compressor components designed for the NASA Ultra Efficient Engine Technology (UEET) program. Initial calculations were used to investigate the physical behavior of VGs. A parametric study of the effects of VG height was done using 3-D calculations of isolated VGs. A body force model was developed to simulate the effects of VGs without requiring complicated grids. The model was calibrated using 2-D calculations of the VG vanes and was validated using the 3-D results. Then three applications of VGs to a compressor rotor and stator were investigated: 1) The results of the 3-D calculations were used to simulate the use of small casing VGs used to generate rotor preswirl or counterswirl. Computed performance maps were used to evaluate the effects of VGs. 2) The body force model was used to simulate large part-span splitters on the casing ahead of the stator. Computed loss buckets showed the effects of the VGs. 3) The body force model was also used to investigate the use of tiny VGs on the stator suction surface for controlling secondary flows. Near-surface particle traces and exit loss profiles were used to evaluate the effects of the VGs.

Chima, R. V.↗

Effects of oxidants and reductants on the efficiency of excitation transfer in green photosynthetic bacteria

The efficiency of energy transfer in chlorosome antennas in the green sulfur bacteria Chlorobium vibrioforme and Chlorobium limicola was found to be highly sensitive to the redox potential of the suspension. Energy transfer efficiencies were measured by comparing the absorption spectrum of the bacteriochlorophyll c or d pigments in the chlorosome to the excitation spectrum for fluorescence arising from the chlorosome baseplate and membrane-bound antenna complexes. The efficiency of energy transfer approaches 100% at low redox potentials induced by addition of sodium dithionite or other strong reductants, and is lowered to 10-20% under aerobic conditions or after addition of a variety of membrane-permeable oxidizing agents. The redox effect on energy transfer is observed in whole cells, isolated membranes and purified chlorosomes, indicating that the modulation of energy transfer efficiency arises within the antenna complexes and is not directly mediated by the redox state of the reaction center. It is proposed that chlorosomes contain a component that acts as a highly quenching center in its oxidized state, but is an inefficient quencher when reduced by endogenous or exogenous reductants. This effect may be a control mechanism that prevents cellular damage resulting from reaction of oxygen with reduced low-potential electron acceptors found in the green sulfur bacteria. The redox modulation effect is not observed in the green gliding bacterium Chloroflexus aurantiacus, which contains chlorosomes but does not contain low-potential electron acceptors.

NASA Discipline Exobiology↗

Solar Wind Strahl Observations and Their Implication to the Core-Halo Formation due to Scattering

A study of the kinetic properties of the strahl electron velocity distribution functions (VDF?s) in the solar wind is presented. This study focuses on the mechanisms that control and regulate the electron VDF?s and the stability of the strahl electrons in the solar wind; mechanisms that are not yet well understood. Various parameters are investigated such as the strahl-electron density, temperature anisotropy, and electron heat-flux. These parameters are used to investigate the stability of the strahl population. The analysis check for whether the strahl electrons are constrained by some instability (e.g., the whistler or KAW instabilities), or are maintained by other types of processes. The electron heat-flux and temperature anisotropy are determined by modeling of the 3D-VDF?s from which the moments properties of the various populations are obtained. The results of this study have profound implication on the current hypothesis about the probable formation of the solar wind halo electrons produced from the scattering of the strahl population. This hypothesis is strengthened by direct observations of the strahl electrons being scattered into the core-halo in an isolated event. The observation implies that the scattering of the strahl is not a continuous process but occurs in bursts in regions where conditions for wave growth providing the scattering are optimum. Sometimes, observations indicate that the strahl component is anisotropic (Tper/Tpal approx. 2). This provides a possible free energy source for the excitation of whistler waves as a possible scattering mechanism, however this condition is not always observed. The study is based on high time resolution data from the Cluster/PEACE electron spectrometer.

Vinas, Adolfo F.↗

Atmosphere Processing Module Automation and Catalyst Durability Analysis for Mars ISRU Pathfinder

The Mars In-Situ Resource Utilization Pathfinder was designed to create fuel using components found in the planet’s atmosphere and regolith for an ascension vehicle to return a potential sample return or crew return vehicle from Mars. The Atmosphere Processing Module (APM), a subunit of the pathfinder, uses cryocoolers to isolate and collect carbon dioxide from Mars simulant gas. The carbon dioxide is fed with hydrogen into a Sabatier reactor where methane is produced. The APM is currently undergoing the final stages of testing at Kennedy Space Center prior to process integration testing with the other subunits of the pathfinder. The automation software for the APM cryocoolers was tested and found to perform nominally. The catalyst used for the Sabatier reactor was investigated to determine the factors contributing to catalyst failure. The results from the catalyst testing require further analysis, but it appears that the rapid change in temperature during reactor start up or the elevated operating temperature is responsible for the changes observed in the catalyst.

Atmosphere Processing Module↗

Results of the 2022 Mental Health Survey of the Planetary Science Community

The mental health struggles within academia and research are well recognized in the science community and have been further illustrated by the numerous cultural memes and comics (e.g., PhD Comics). With the recent COVID-19 pandemic, mental health has become a topic of concern more so than ever before, as depression and anxiety have become an invisible consequence of social isolation and distancing. In addition, recent social events in the United States have reignited attention towards important social injustices surrounding people of color, women, and LGBTQ+ in academia. If diversity, equity, inclusion, and accessibility are to become major components of improving planetary science, it is also important to understand the mental health status of the community, as it could expose deeply embedded policies, rules, and culture that may hinder any work in sustaining and advancing people belonging to marginalized groups. Studies have been conducted to further understand the mental health of those in academia. These studies have shown that before the pandemic, there was a mental health crisis among graduate students, which became a larger problem with increasing anxiety and depression due to the pandemic. Soon after, the planetary science community recognized this concern in the recent 2023 Planetary Science Decadal White Paper, which proposed that NASA should invest in understanding the scope and impact of mental health problems within the planetary science community and how to address the issue. In this study, we conducted a mental health survey to examine the mental health of the planetary science community. We examined the overall anxiety, depression, and stress severity of the community, rather than diagnosing the community’s condition or determining the proportion of the population having a clinical anxiety, depressive, stress, or trauma-related diagnosis. To emphasize, the results are not intended to make clinical diagnoses, nor are they being used to do so for the purpose of this study.

D Trang↗

Social Isolation Impacts Select Responses to Simulated Weightlessness

The rodent hindlimb unloading (HU) model was initially developed to simulate the cephalad fluid shift and musculoskeletal disuse in astronauts. Since then, the HU model has been applied to explore how other systems (e.g. immune, cardiovascular and CNS) respond to weightlessness. Most HU studies are performed with singly-housed animals, although social isolation also can substantially impact behavior and physiology, and therefore may confound HU experimental results. We hypothesized that relative to social housing, single housing exacerbates HU-induced dysfunction in select organ systems. We refined the standard NASA-Ames HU model to accommodate social housing in HU pairs, retaining advantageous features of traditional housing but using commercial off-the-shelf components to facilitate adoption by others. We conducted a 30 day HU experiment with adult, female C57Bl6/NJ mice that were either singly or socially housed. HU animals in both single and social HU housing displayed expected musculoskeletal deficits compared to housing matched, normally loaded (NL) controls. However, select immune, HPA axis, and CNS responses were differentially impacted by the HU social environment relative to NL controls. HU reduced % CD4+ T cells in singly-housed, but not socially-housed mice. Surprisingly, HU increased adrenal gland mass in socially-housed but not singly-housed mice, while social isolation increased adrenal gland mass in NL controls. HU also increased plasma corticosterone levels (day 30) in both singly and socially-housed mice. Thus, the social environment altered select adrenal and immune, but not musculoskeletal, responses to simulated weightlessness. We refine our original hypothesis since our results show combined stressors can mask, not only exacerbate, tissue responses to HU. These findings further expand the utility of the HU model for studying possible combined effects of the various spaceflight stressors.

Tahimic, Candice↗

Implementation of natural frequency analysis and optimality criterion design

A description is presented of an effective optimality criterion computer design approach for member size selection to improve frequency characteristics for moderately large structure models. It is shown that the implementation of the simultaneous iteration method within a natural frequency structural design optimization provides a method which is more efficient in isolating the lowest natural frequency modes than the frequently applied Stodola method. Additional computational advantages are derived by using previously converged eigenvectors at the start of the iterations during the second and the following design cycles. Vectors with random components can be used at the first design cycle, which, in relation to the entire computer time for the design program, results in only a moderate computational penalty.

Levy, R.↗

Structure and evolution of isolated giant gaseous protoplanets

A model is developed describing the structure and evolution of isolated giant gaseous protoplanets in the mass range 0.3-4.5 Jovian masses. The central region of protoplanets of mass less than about 1 Jovian mass is, at some evolutionary epoch, thermodynamically favorable to the liquefaction of major interstellar grain components. All protoplanets studied are convective throughout most of their interior. In addition, it was found that the thermal contraction time depends sensitively on the surface opacity (at T less than 200 K).

Decampli, W. M.↗

Transient motion of thick anisotropic plates

Analyses are developed for the response of anisotropic plate strips to a transient load. The load is taken in the form of a line load of normal stress on the surface or within the body of the strip. The characteristic free vibrational modes of the strip are derived and used to derive the secular equation for this case in closed form and to isolate the mathematical conditions for symmetric and antisymmetric wave mode propagation in completely separate terms. The applied loads are expanded in terms of these normal modes and the response of the plate is obtained by superposition of the appropriate components. Material systems of higher symmetry are contained implicitly in the analysis.

Nayfeh, Adnan H.↗

Intelligent control of a multi-degree-of freedom reaction compensating platform system using fuzzy logic

This paper presents the development of a general-purpose fuzzy logic (FL) control methodology for isolating the external vibratory disturbances of space-based devices. According to the desired performance specifications, a full investigation regarding the development of an FL controller was done using different scenarios, such as variances of passive reaction-compensating components and external disturbance load. It was shown that the proposed FL controller is robust in that the FL-controlled system closely follows the prespecified ideal reference model. The comparative study also reveals that the FL-controlled system achieves significant improvement in reducing vibrations over passive systems.

Choi, Benjamin B.↗

From Research to Operations: Integrating Components of an Advanced Diagnostic System with an Aspect-Oriented Framework

This paper presents some of the challenges associated with bringing software projects from the research world into an operationa1 environment. While the core functional components of research-oriented software applications can have great utility in an operational setting, these applications often lack aspects important in an operational environment such as logging and security. Furthermore, these stand-alone applications, sometimes developed in isolation from one another, can produce data products useful to other applications in a software ecosystem.

Fletcher, Daryl P.↗

Organization of photosystem I polypeptides examined by chemical cross-linking

Photosystem I from the cyanobacterium Synechocystis sp. PCC 6803 was examined using the chemical cross-linkers glutaraldehyde and N-ethyl-1-3-[3-(dimethylamino)propyl]carbodiimide to investigate the organization of the polypeptide subunits. Thylakoid membranes and photosystem I, which was isolated by Triton X-100 fractionation, were treated with cross-linking reagents and were resolved using a Tricine/urea low-molecular-weight resolution gel system. Subunit-specific antibodies and western blotting analysis were used to identify the components of cross-linked species. These analyses identified glutaraldehyde-dependent cross-linking products composed of small amounts of PsaD and PsaC, PsaC and PsaE, and PsaE and PsaF. The novel cross-link between PsaE and PsaF was also observed following treatment with N-ethyl-1-3-[3-(dimethylamino)propyl]carbodiimide. These cross-linking results suggest a structural interaction between PsaE and PsaF and predict a transmembrane topology for PsaF.

NASA Discipline Plant Biology↗

Evaluating Attenuation of Vibration Response using Particle Impact Damping for a Range of Equipment Assemblies

Particle dampers provide a mechanism for diverting energy away from resonant structural vibrations. This experimental study provides data from a series of acoustically excited tests to determine the effectiveness of these dampers for equipment mounted to a curved orthogrid panel for a launch vehicle application. Vibration attenuation trends are examined for variations in particle damper fill level, component mass, and excitation energy. A significant response reduction at the component level was achieved, suggesting that comparatively small, strategically placed, particle damper devices might be advantageously used in launch vehicle design. These test results were compared to baseline acoustic response tests without particle damping devices, over a range of isolation and damping parameters. Instrumentation consisting of accelerometers, microphones, and still photography data will be collected to correlate with the analytical results.

Knight, Brent↗