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At least 649 records · Page 36

From the bench to the reactor: engineered filamentous fungi for biochemical and biomaterial production

Filamentous fungi can convert a wide variety of naturally occurring chemical compounds, including organic biomass and waste streams, into a range of products. They have long been used for industrial organic acid production and food preparation. In this review, we will discuss production of products such as organic acids, lipids, small molecules, enzymes, materials, and foods, and highlight advances in metabolic and protein engineering, including CRISPR-Cas9-mediated strain improvements. We discuss to what extent these products are already being made on a commercial scale, as well as what is still required to make certain promising concepts industrially and commercially relevant. Despite significant progress, the systematic application of synthetic biology to filamentous fungi remains in its infancy, with many opportunities for discovery and innovation as new strains and genetic tools are developed. The integration of fungal biotechnology into circular and bio-based economies promises to address critical challenges in waste management, resource sustainability, and the development of new materials for terrestrial and extraterrestrial applications, but requires further developments in genetic engineering and process design.

09 BIOMASS FUELS↗

Optimization of microwave emission from laser filamentation with a machine learning algorithm

We demonstrate that is it possible to optimize the yield of microwave radiation from plasmas generated by laser filamentation in atmosphere through manipulation of the laser wavefront. A genetic algorithm controls a deformable mirror that reconfigures the wavefront using the microwave waveform amplitude as feedback. Optimization runs performed as a function of air pressure show that the genetic algorithm can double the microwave field strength relative to when the mirror surface is flat. Here, an increase in the volume and brightness of the plasma fluorescence accompanies the increase in microwave radiation, implying an improvement in the laser beam intensity profile through the filamentation region due to the optimized wavefront.

Englesbe, Alexander↗

Millisecond-long suppression of spectroscopic optical signals using laser filamentation

Ultrashort laser pulse filamentation in air can extend the delivery of focused laser energy to distances greatly exceeding the Rayleigh length. In this way, remote measurements can be conducted using many standard methods of analytical spectroscopy. The performance of spectroscopic techniques can be enhanced by temporal gating, which rejects the unwanted noise and background. In the present work, we investigate the thermal relaxation of air in the wake of single-filament plasmas using shadowgraphy. Here, we demonstrate that the transient change in refractive index associated with relaxation of the gas can be used to reject both continuous and time-varying spectroscopic signals, including emission from laser-produced plasmas. This method can augment temporal gating of simple optical detectors.

47 OTHER INSTRUMENTATION↗

Enhancing Plastic Recycling Through Nano-Scale Structure Analysis in Custom Block Copolymer Filaments (Rev.1)

As plastic production continues to increase, new methods to combat plastic waste are needed. This paper explores upcycling, a way to increase the utility or value of waste plastic. This can be done using block copolymers as compatibilizers. Block copolymers are made of two immiscible polymer blocks that phase separate on the nanoscale, forming small structures. The orientation of these nano-scale structures influences mechanical properties, and we show that orientation can be controlled by directional 3D (three dimensional) printing. Using small-angle X-ray scattering (SAXS), we analyze block copolymer filaments extruded at varying speeds. The SAXS data provide a baseline understanding of nano-scale structure in these unique filaments for future use in 3D printing experiments.

36 MATERIALS SCIENCE↗

Understanding Radiotropism in Filamentous Fungi

Melanized species of filamentous fungi isolated from high radiation environments have been reported to exhibit radiotropism, defined as the directed growth toward a source of ionizing radiation. Inconsistencies in the experimental approaches and results have impeded our understanding of the key factors involved in radiotropism. In the present study, we assessed radiotropism in four isolates of fungi: Aspergillus niger, A. calidoustus JC-1043, Paecilomyces variotii SNL-1, and P. variotii IMV-00236. Of these fungi, only P. variotii IMV-00236 had been previously reported to exhibit radiotropic behavior. Plates of each fungus were placed in equivalent proximity to a 137 Cs source, with a primary gamma emission of 662 keV, and differences in the rate and direction of mycelia growth were measured over a seven-day period. Significant differences were not observed in the rate or direction of growth of the different fungi based on exposure to gamma radiation, which suggested a lack of measurable radiotropism in these experiments. Additional studies varying parameters such gamma emission rates and energies, as well as other types of ionizing radiation (e.g., alpha and beta particles, neutrons) are necessary to gain further insights to the factors critical to the expression of radiotropic behavior in filamentous fungi.

59 BASIC BIOLOGICAL SCIENCES↗

Breaking Beams: A Kinetic Treatment of the Filamentation and Forward Scatter of Laser Beams in Plasma (Abbreviated Final Report)

Precise and predictable propagation and deposition of laser energy in high energy density (HED) plasma is threatened by the breakup of the beam into filaments. Such filaments can be deflected and sprayed but also locally intensify the light, increasing the likelihood of other problematic interactions with the plasma. Adequately modeling, anticipating, and avoiding such processes in HED experiments requires a thorough understanding of the thresholds and nonlinear saturation mechanisms, particularly for the weakly collisional, high temperature plasmas achieved at the National Ignition Facility (NIF).

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

The effect of inhomogeneities in coal-based mesophase pitch on filament formation and melt spinning for carbon fiber

Mesophase pitch-based carbon fibers excel as high specific strength and modulus materials, but challenges in stable, continuous melt spinning processing are a serious limiting factor. Inhomogeneities in the pitch may result in flow instabilities that can lead to filament breakage. This work aims to characterize and offer mitigation strategies for the negative effects that inhomogeneities such as isotropic binder, gels, and other inclusions impart on melt spinning of mesophase pitch. The effect of inhomogeneities on spinning stability are analyzed using capillary rheometry, borescope imaging of elongating filaments, and filtration. With no filter, immediate blockage of the spinning capillary occurred. Improvement in melt spinning duration was observed when a porous metal filter (20 μm nominal pore size) was utilized, but no significant further improvement in spinning duration was found upon decreasing the filter nominal pores size to 10, 5 and 2 μm. Moreover, flow instabilities that disrupted melt spinning stability were observed by capillary rheometry when the size of included isotropic pitch domains were larger than approximately 10 μm. Resultant carbon fiber properties and SEM imaging of fracture surfaces are reported.

01 COAL, LIGNITE, AND PEAT↗

Breaking Beams: A Kinetic Treatment of the Filamentation and Forward Scatter of Laser Beams in Plasma

Precise and predictable propagation and deposition of laser energy in high energy density (HED) plasma is threatened by the breakup of the beam into filaments. Such filaments can be deflected and sprayed but also locally intensify the light, increasing the likelihood of other problematic interactions with the plasma. Adequately modeling, anticipating, and avoiding such processes in HED experiments requires a thorough understanding of the thresholds and nonlinear saturation mechanisms, particularly for the weakly collisional, high temperature plasmas achieved at the National Ignition Facility (NIF).

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Magnetic field strength in an intermediate-velocity ionized filament in the first galactic quadrant

We investigate the magnetic field in an intermediate-velocity filament for which the Hα intensity in the WHAM survey correlates with excess Faraday rotation of extragalactic radio sources over the length of the filament from b ≈ 20° to b ≈ 55°. The density-weighted mean magnetic field is 2.8 ±0.8 μG, derived from rotation measures and an empirical relation between Hα emission measure and dispersion measure from Berkhuijsen et al. In view of the uncertainties in the derived magnetic field strength, we propose an alternative use of the available data, rotation measure, and emission measure, to derive a lower limit to the Alfvén speed, weighted by electron density n{sub e}{sup 3/2}. We find lower limits to the Alfvén speed that are comparable to or larger than the sound speed in a 10{sup 4} K plasma, and conclude that the magnetic field is dynamically important. We discuss the role of intermediate-velocity gas as a locus of Faraday rotation in the interstellar medium, and propose that this lower limit to the Alfvén speed may also be applicable to Faraday rotation by galaxy clusters.

79 ASTRONOMY AND ASTROPHYSICS↗

MINI-FILAMENT ERUPTION AS THE INITIATION OF A JET ALONG CORONAL LOOPS

Minifilament eruptions (MFEs) and coronal jets are different types of solar small-scale explosive events. We report an MFE observed at the New Vacuum Solar Telescope (NVST). As seen in the NVST H α images, during the rising phase, the minifilament erupts outward orthogonally to its length, accompanied with a flare-like brightening at the bottom. Afterward, dark materials are found to possibly extend along the axis of the expanded filament body. The MFE is analogous to large filament eruptions. However, a simultaneous observation of the Solar Dynamics Observatory shows that a jet is initiated and flows out along nearby coronal loops during the rising phase of the MFE. Meanwhile, small hot loops, which connect the original eruptive site of the minifilament to the footpoints of the coronal loops, are formed successively. A differential emission measure analysis demonstrates that, on the top of the new small loops, a hot cusp structure exists. We conjecture that the magnetic fields of the MFE interact with magnetic fields of the coronal loops. This interaction is interpreted as magnetic reconnection that produces the jet and the small hot loops.

79 ASTRONOMY AND ASTROPHYSICS↗

The JCMT BISTRO Survey: The Magnetic Field Strength in the Orion A Filament

We determine the magnetic field strength in the OMC 1 region of the Orion A filament via a new implementation of the Chandrasekhar–Fermi method using observations performed as part of the James Clerk Maxwell Telescope (JCMT) B-Fields In Star-forming Region Observations (BISTRO) survey with the POL-2 instrument. We combine BISTRO data with archival SCUBA-2 and HARP observations to find a plane-of-sky magnetic field strength in OMC 1 of B{sub pos}=6.6±4.7 mG, where δB{sub pos}=4.7 mG represents a predominantly systematic uncertainty. We develop a new method for measuring angular dispersion, analogous to unsharp masking. We find a magnetic energy density of ∼1.7×10{sup −7} J m{sup −3} in OMC 1, comparable both to the gravitational potential energy density of OMC 1 (∼10{sup −7} J m{sup −3}) and to the energy density in the Orion BN/KL outflow (∼10{sup −7} J m{sup −3}). We find that neither the Alfvén velocity in OMC 1 nor the velocity of the super-Alfvénic outflow ejecta is sufficiently large for the BN/KL outflow to have caused large-scale distortion of the local magnetic field in the ∼500 yr lifetime of the outflow. Hence, we propose that the hourglass field morphology in OMC 1 is caused by the distortion of a primordial cylindrically symmetric magnetic field by the gravitational fragmentation of the filament and/or the gravitational interaction of the BN/KL and S clumps. We find that OMC 1 is currently in or near magnetically supported equilibrium, and that the current large-scale morphology of the BN/KL outflow is regulated by the geometry of the magnetic field in OMC 1, and not vice versa.

79 ASTRONOMY AND ASTROPHYSICS↗

Balmer Filaments in Tycho’s Supernova Remnant: An Interplay between Cosmic-ray and Broad-neutral Precursors

We present Hα spectroscopic observations and detailed modeling of the Balmer filaments in the supernova remnant (SNR) Tycho (SN 1572). We used GH α FaS (Galaxy Hα Fabry–Pérot Spectrometer) on the William Herschel Telescope with a 3.′4 × 3.′4 field of view, 0.″2 pixel scale, and σ{sub instr}=8.1 km s{sup −1} resolution at 1″ seeing for ∼10 hr, resulting in 82 spatial−spectral bins that resolve the narrow Hα line in the entire SN 1572 northeastern rim. For the first time, we can therefore mitigate artificial line broadening from unresolved differential motion and probe Hα emission parameters in varying shock and ambient medium conditions. Broad Hα line remains unresolved within spectral coverage of 392 km s{sup −1}. We employed Bayesian inference to obtain reliable parameter confidence intervals and to quantify the evidence for models with multiple line components. The median Hα narrow-line (NL) FWHM of all bins and models is W{sub NL}=(54.8±1.8) km s{sup −1} at the 95% confidence level, varying within [35, 72] km s{sup −1} between bins and clearly broadened compared to the intrinsic (thermal) ≈20 km s{sup −1}. Possible line splits are accounted for, significant in ≈18% of the filament, and presumably due to remaining projection effects. We also find widespread evidence for intermediate-line emission of a broad-neutral precursor, with a median W{sub IL}=(180±14) km s{sup −1} (95% confidence). Finally, we present a measurement of the remnant’s systemic velocity, V{sub LSR}=−34 km s{sup −1}, and map differential line-of-sight motions. Our results confirm the existence and interplay of shock precursors in Tycho’s remnant. In particular, we show that suprathermal NL emission is near-universal in SN 1572, and that, in the absence of an alternative explanation, collisionless SNR shocks constitute a viable acceleration source for Galactic TeV cosmic-ray protons.

79 ASTRONOMY AND ASTROPHYSICS↗

RF beam scattering by cylindrical filaments and interfacial density fluctuations

Radio Frequency (RF) waves are routinely used in tokamaks for heating, current drive, NTM control, as well as for diagnostics purposes. Frequently, RF waves, aiming towards the plasma core, propagate through a turbulent environment. The latter can exhibit strong coherent density fluctuations as well as filamentary structures mainly (though, not perfectly) aligned along the local magnetic field lines. The scattering process of RF waves by these structures is studied both analytically and numerically. RF waves can be either single plane waves or spatially confined beams. For that purpose, the filaments are considered to have cylindrical shape with infinite length with the cylinder axis not aligned with the local magnetic field and the results are compared to the ones from the study of the aligned case. On the other hand, the interfacial density fluctuations are considered periodic with spatial periods larger, smaller or of the same order of the wavelength of the incident RF waves. The frequency range of the RF waves studied is mainly in the Electron Cyclotron (EC) range of frequencies for ITER-like and Medium Size Tokamak applications. Furthermore, the study covers a variety of density contrasts, filament sizes and fluctuation strengths.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Analysis of filament reinforced metal-shell pressure vessels

Computer program analyzes design requirements and computes designs for metal-lined filament-wound pressure vessels with either geodesic /helical/ or in-plane filament winding patterns on the cylindrical portion and over the ends, reinforced by circumferential windings on the cylindrical portion.

Landes, R. E.↗

Cycle testing of boron filament wound tanks

Filament tensile strengths and pressure-strain characteristics of high modulus, high-strength, boron-filament-wound/resin-composite pressure vessel at ambient and cryogenic temperatures

Alfring, R. J.↗

Filament-wound, fiberglass cryogenic tank supports

The design, fabrication, and testing of filament-wound, fiberglass cryogenic tank supports for a LH2 tank, a LF2/FLOX tank and a CH4 tank. These supports consist of filament-wound fiberglass tubes with titanium end fittings. These units were satisfactorily tested at cryogenic temperatures, thereby offering a design that can be reliably and economically produced in large or small quantities. The basic design concept is applicable to any situation where strong, lightweight axial load members are desired.

Carter, J. S.↗

High performance filament wound composites for pressure vessel applications.

A new high modulus fiber was evaluated as a reinforcement for filament wound pressure vessels. Based on preliminary data it offers significant specific strength and modulus improvements over an S-glass reinforcement. Winding parameters and design data were established for the fiber with two different epoxy resin systems. NOL composite rings were evaluated for tensile strength, modulus and interlaminar shear strength at +70 F, -320 F and -423 F. Results showed that the fiber reinforced composite exhibited a specific strength of 4,100,000 inches and a specific modulus of 290,000,000 inches compared to 3,260,000 inches and 110,000,000 inches respectively for S-glass. Utilizing this data to design small filament wound pressure vessels, a performance factor of 806,000 inches was obtained experimentally with PRD 49-1 fiber compared to values of 632,000 inches and 501,000 inches for S-glass and high modulus graphite vessels, respectively.

Hoggatt, J. T.↗