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NASA NTRS2003

Compaction agent clarification of microbial lysates

Recombinant proteins are often purified from microbial lysates containing high concentrations of nucleic acids. Pre-purification steps such as nuclease addition or precipitation with polyethyleneimine or ammonium sulfate are normally required to reduce viscosity and to eliminate competing polyanions before anion exchange chromatography. We report that small polycationic compaction agents such as spermine selectively precipitate nucleic acids during or after Escherichia coli lysis, allowing DNA and RNA to be pelleted with the insoluble cell debris. Analysis by spectrophotometry and protein assay confirmed a significant reduction in the concentration of nucleic acids present, with preservation of protein. Lysate viscosity is greatly reduced, facilitating subsequent processing. We have used 5mM spermine to remove nucleic acids from E. coli lysate in the purification of a hexahistidine-tagged HIV reverse transcriptase.

NASA Discipline Environmental Health↗

NASA NTRS1987

Collection of gravitropic effectors from mucilage of electrotropically-stimulated roots of Zea mays L

We placed agar blocks adjacent to tips of electrotropically stimulated primary roots of Zea mays. Blocks placed adjacent to the anode-side of the roots for 3 h induced significant curvature when subsequently placed asymmetrically on tips of vertically-oriented roots. Curvature was always toward the side of the root unto which the agar block was placed. Agar blocks not contacting roots and blocks placed adjacent to the cathode-side of electrotropically stimulated roots did not induce significant curvature when placed asymmetrically on tips of vertically-oriented roots. Atomic absorption spectrophotometry indicated that blocks adjacent to the anode-side of electrotropically-stimulated roots contained significantly more calcium than (1) blocks not contacting roots, and (2) blocks contacting the cathode-side of roots. These results demonstrate the presence of a gradient of endogenous Ca in mucilage of electrotropically-stimulated roots (i.e. roots undergoing gravitropic-like curvature).

NASA Discipline Number 40-10↗

NASA NTRS2004

The Swift Gamma Ray Burst Mission

Swift is an international mission managed by NASA as part of its MIDEX program. It is a multiwavelength transient observatory for GRB astronomy that will launch in 2004. The goals of the mission are to determine the origin of GRBs and their afterglows and use bursts to probe the early Universe. A wide field gamma-ray camera will detect more than a hundred GRBs per year to 2-5 times fainter than BATSE. Sensitive narrow-field X-ray, and UV/optical telescopes will be pointed at the burst location in 20 to 75 sec by an autonomously controlled 'swift' spacecraft. For each burst, arcsec positions will be determined and optical/UV/x-ray/gamma-ray spectrophotometry performed. Measurements of redshift will be made for many of the bursts. The instrumentation is a combination of superb existing flight-spare hardware and design from XMM and Spectrum-X/JET-X contributed by collaborators in the UK and Italy and development of a coded-aperture camera with a large-area (approx. 0.5 square meter) CdZnTe detector array. The instruments have now completed their fabrication phase and are integrated on the observatory for final testing. Key components of the mission are vigorous follow-up and outreach programs to engage the astronomical community and public in Swift. The talk will describe the mission and its status and give a summary of our plans for GRB operations.

Gehrels, Neil↗

NASA NTRS2004

The Swift GRB Mission

Swift is a MIDEX mission that is in development for launch in October 2004. It is a multiwavelength transient observatory for GRB astronomy. The goals of the mission are to determine the origin of GRBs and their afterglows and use bursts to probe the early Universe. A wide-field gamma-ray camera will detect mare than 100 GRBs per year to -3 times fainter than BATSE. Sensitive narrow-field X-ray and UV/optical telescopes will be pointed at the burst location in 20 to 75 sec by an autonomously controlled spacecraft. Far each burst, aresec positions will be determined and optical/UV/X-ray/gamma-say spectrophotometry performed. Measurements of redshift will be made for many burstes. The instrumentation is a combination of superb existing flight-spare hardware and design from XMM and Spectrum-X/JET-X contributed by collaborators in the UK and Italy and development of a coded-aperture camera with a large-area (approx. 0.5 square meter) CdZnTe detector array. Key components of the mission are vigorous follow-up and outreach programs to engage the astronomical community and public in Swift. The talk vi11 describe the mission statue and give a summary of plans for GRB operations. It is likely that Swift will have just been launched at the time of the conference.

Gehrels, Neil↗

NASA NTRS2004

The Imminent Swift MIDEX Mission

Swift is a NASA MIDEX mission that is in development for launch in Fall 2004. It is a multiwavelength observatory for transient astronomy. The goals of the mission are to determine the origin of gamma-ray bursts and their afterglows and use bursts to probe the early Universe. The mission will also perform a hard x-ray survey at the 1 milliCrab level and will continuously monitor the sky for transients. A wide-field gamma-ray camera will detect more than a hundred GRBs per year to 3 times fainter than BATSE. Sensitive narrow-field X-ray and UV/optical telescopes will be pointed at the burst. location in 20 to 70 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions will be determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. The instrumentation is a combination of existing flight-spare hardware and design from XMM and Spectrum-X/JET-X contributed by collaborators in the UK and Italy and development of a coded-aperture camera with a large-area (approximately 0.5 square meter) CdZnTe detector array. The ground station in Malindi is contributed by the Italian Space Agency. The instruments have now completed their fabrication phase and are currently being integrated on the observatory for final testing. Key components of the mission are vigorous follow-up and outreach programs to engage the astronomical community and public in Swift.

Gehrels, Neil↗

NASA NTRS2003

Spectroscopy and Photometry of Mars Trojans

Mars is the only terrestrial planet known to have co-orbiting Trojan asteroids. We have obtained visible and near-IR reflectance spectra of three of these objects: 5261 Eureka and 1998 VF31 in the L5 region and 1999 UJ7 in the LA region. We also obtained JHK spectrophotometry and a visible lightcurve for 5261 Eureka. The asteroid 5261 Eureka has a visible spectrum that is classified as Sr in the Bus taxonomy, and has infrared colors consistent with the A-class asteroids. The data for 1998 VF31 have a restricted wavelength range, but are most consistent with the Sr or Sa class, though we note a marginal consistency with the D class. We can rule out a C-class classification. 1999 UJ7 has an X-class or T-class spectrum, which is unlike that of the other two Mars Trojans. The photometric data for Eureka are limited, but we can constrain the period to longer than 5 hours (likely 5.5-6 hours) and lightcurve amplitude of at least 0.15 magnitude at this viewing geometry. The spectral differences among the Mars Trojans suggests that either they did not all form at their present solar distances or that they have not always been at their present sizes.

Rivkin, A. S.↗

NASA NTRS2005

The Swift Gamna-Ray Burst Mission

Swift is a NASA Explorer mission that will be launched in late 2004. It is a multiwavelength observatory for transient astronomy. The goals of the mission are to determine the origin of gamma-ray bursts and their afterglows and use bursts to probe the early Universe. The mission will also perform a hard x-ray survey at the 1 milliCrab level and will continuously monitor the sky for transients. A wide-field gamma-ray camera will detect more than a hundred GRBs per year to 3 times fainter than BATSE. Sensitive narrow-field X-ray and UV/optical telescopes will be pointed at the burst location in 20 to 70 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions will be determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. The instrumentation is a combination of existing flight-spare hardware and design from XMM and Spectrum-X/JET-X contributed by collaborators in the UK and Italy and development of a coded-aperture camera with a large-area (-0.5 square meter) CdZnTe detector array. The ground station in Malindi is contributed by the Italian Space Agency. Key components of the mission are vigorous follow-up and outreach programs to engage the astronomical community and public in Swift.

Gehrels, Neil↗

NASA NTRS2005

Mineralogy and Temperature-induced Spectral Investigations of A-type Asteroids 246 Asporina and 446 Aeternitas

A-type asteroids are a relatively rare taxonomic class with no more than 17 known objects. They were first identified as a separate group of R-type asteroids based on broadband spectrophotometry by, and were later classified based on ECAS data by Tholen (1984). These asteroids have moderately high albedos (0.13-0.39), extremely reddish slopes shortward of 0.7 m and a strong absorption feature centered at approx. 1.05 m. More recent surveys like the Small Main-Belt Asteroid Spectroscopic Survey (SMASS) and SMASS II have expanded the taxonomic classes including the A-type, adding 12 new asteroids to the original five.

Reddy, V.↗

NASA NTRS2005

First Results from the Swift Gamma Ray Burst Mission

Swift is now in orbit after a beautiful launch on November 20, 2004. It is a multiwavelength observatory designed specifically to study the fascinating gamma-ray bursts. The goals are to determine the origin of bursts and use them to probe the early Universe. A new-technology wide-field gamma-ray camera detects more than a hundred bursts per year. Sensitive narrow-field X-ray and UV/optical telescopes are pointed at the burst location in 20 to 70 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions are determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. Information is also rapidly sent to the ground to a team of more than 50 observers at telescopes around the world. First results from the mission will be presented, including observations of bright GRBs, faint GRBs, short GRBs and a super-giant flare from the soft gamma repeater SGRl806-20.

Gehreis, Neil↗

NASA NTRS2001

Light Microscopy Module: An On-Orbit Microscope Planned for the Fluids and Combustion Facility on the International Space Station

The Light Microscopy Module (LMM) is planned as a fully remotely controllable on-orbit microscope subrack facility, allowing flexible scheduling and control of fluids and biology experiments within NASA Glenn Research Center's Fluids and Combustion Facility on the International Space Station. Within the Fluids and Combustion Facility, four fluids physics experiments will utilize an instrument built around a light microscope. These experiments are the Constrained Vapor Bubble experiment (Peter C. Wayner of Rensselaer Polytechnic Institute), the Physics of Hard Spheres Experiment-2 (Paul M. Chaikin of Princeton University), the Physics of Colloids in Space-2 experiment (David A. Weitz of Harvard University), and the Low Volume Fraction Colloidal Assembly experiment (Arjun G. Yodh of the University of Pennsylvania). The first experiment investigates heat conductance in microgravity as a function of liquid volume and heat flow rate to determine, in detail, the transport process characteristics in a curved liquid film. The other three experiments investigate various complementary aspects of the nucleation, growth, structure, and properties of colloidal crystals in microgravity and the effects of micromanipulation upon their properties. Key diagnostic capabilities for meeting the science requirements of the four experiments include video microscopy to observe sample features including basic structures and dynamics, interferometry to measure vapor bubble thin film thickness, laser tweezers for colloidal particle manipulation and patterning, confocal microscopy to provide enhanced three-dimensional visualization of colloidal structures, and spectrophotometry to measure colloidal crystal photonic properties.

Doherty, Michael P.↗

NASA NTRS2003

Light Microscopy Module Fan Disturbance Characterized Through Microgravity Emissions Laboratory Testing

A Light Microscopy Module (LMM) is being engineered, designed, and developed at the NASA Glenn Research Center. The LMM is planned as a remotely controllable on-orbit microscope subrack facility, allowing flexible scheduling and control of physical science and biological science experiments within Glenn s Fluids Integrated Rack on the International Space Station. The LMM concept is a modified commercial research imaging light microscope with powerful laser-diagnostic hardware and interfaces, creating a one-of-a-kind, state-of-the-art microscopic research facility. The microscope will house several different objectives, corresponding to magnifications of 10, 40, 50, 63, and 100. Features of the LMM include high-resolution color video microscopy, brightfield, darkfield, phase contrast, differential interference contrast, spectrophotometry, and confocal microscopy combined in a single configuration. Also, laser tweezers are integrated with the diagnostics as a sample manipulation technique. As part of the development phase of the LMM, it was necessary to quantify the microgravity disturbances generated by the control box fan. Isolating the fan was deemed necessary to reduce the fan speed harmonic amplitudes and to eliminate any broadband disturbances across the 60- to 70-Hz and 160- to 170-Hz frequency ranges. The accelerations generated by a control box fan component of the LMM were measured in the Microgravity Emissions Laboratory (MEL). The MEL is a low-frequency measurement system developed to simulate and verify the on-orbit International Space Station (ISS) microgravity environment. The accelerations generated by various operating components of the ISS, if too large, could hinder the science performed onboard by disturbing the microgravity environment. The MEL facility gives customers a test-verified way of measuring their compliance with ISS limitations on vibratory disturbance levels. The facility is unique in that inertial forces in 6 degrees of freedom can be characterized simultaneously for an operating test article. Vibratory disturbance levels are measured for engineering or flight-level hardware following development from component to subassembly through the rack-level configuration. The MEL can measure accelerations as small as 10-7g, the accuracy needed to confirm compliance with ISS requirements.

McNelis, Anne M.↗

NASA NTRS2004

Light Microscopy Module Imaging Tested and Demonstrated

The Fluids Integrated Rack (FIR), a facility-class payload, and the Light Microscopy Module (LMM), a subrack payload, are integrated research facilities that will fly in the U.S. Laboratory module, Destiny, aboard the International Space Station. Both facilities are being engineered, designed, and developed at the NASA Glenn Research Center by Northrop Grumman Information Technology. The FIR is a modular, multiuser scientific research facility that is one of two racks that make up the Fluids and Combustion Facility (the other being the Combustion Integrated Rack). The FIR has a large volume dedicated for experimental hardware; easily reconfigurable diagnostics, power, and data systems that allow for unique experiment configurations; and customizable software. The FIR will also provide imagers, light sources, power management and control, command and data handling for facility and experiment hardware, and data processing and storage. The first payload in the FIR will be the LMM. The LMM integrated with the FIR is a remotely controllable, automated, on-orbit microscope subrack facility, with key diagnostic capabilities for meeting science requirements--including video microscopy to observe microscopic phenonema and dynamic interactions, interferometry to make thin-film measurements with nanometer resolution, laser tweezers to manipulate micrometer-sized particles, confocal microscopy to provide enhanced three-dimensional visualization of structures, and spectrophotometry to measure the photonic properties of materials. Vibration disturbances were identified early in the LMM development phase as a high risk for contaminating the science microgravity environment. An integrated FIR-LMM test was conducted in Glenn's Acoustics Test Laboratory to assess mechanical sources of vibration and their impact to microscopic imaging. The primary purpose of the test was to characterize the LMM response at the sample location, the x-y stage within the microscope, to vibration emissions from the FIR and LMM support structures.

Gati, Frank↗

NASA NTRS2006

Gamma Ray Burst Discoveries by the Swift Mission

Gamma-ray bursts are among the most fascinating occurrences in the cosmos. They are thought to be the birth cries of black holes throughout the universe. The NASA Swift mission is an innovative new multiwavelength observatory designed to determine the origin of bursts and use them to probe the early Universe. Swift is now in orbit after a beautiful launch on November 20, 2004. A new-technology wide-field gamma-ray camera detects more than a hundred bursts per year. Sensitive narrow-field X-ray and UV/optical telescopes, built in collaboration with UK and Italian partners, are pointed at the burst location in 20 to 70 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions are determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. Information is also rapidly sent to the ground to a team of more than 50 observers at telescopes around the world. The first year of findings from the mission will be presented. The long-standing mystery of short GRBs has been solved, and the answer is the most interesting possible scenario. High redshift bursts have been detected leading to a better understanding of star formation rates and distant galaxy environments. GRBs have been found with giant X-ray flares occurring in their afterglow. These, and other topics, will be discussed.

Gehrels, Neil↗

NASA NTRS2006

Gamma Ray Burst Discoveries by the Swift Mission

Gamma-ray bursts are among the most fascinating occurrences in the cosmos. They are thought to be the birth cries of black holes throughout the universe. The NASA Swift mission is an innovative new multiwavelength observatory designed to determine the origin of bursts and use them to probe the early Universe. Swift is now in orbit after a beautiful launch on November 20, 2004. A new-technology wide-field gamma-ray camera detects more than a hundred bursts per year. Sensitive narrow-field X-ray and UV/optical telescopes, built in collaboration with UK and Italian partners and calibrated in Germany, are pointed at the burst location in 20 to 70 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions are determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. Information is also rapidly sent to the ground to a team of more than 50 observers at telescopes around the world. Early results from the mission will be presented. The long-standing mystery of short GRBs has been solved, and the answer is the most interesting possible scenario. High redshift bursts have been detected leading to a better understanding of star formation rates and distant galaxy environments. GRBs have been found with giant X-ray flares occurring in their afterglow. Observations of magnetars, galactic transients, supernovae, AGN and many other types of sources are also being performed

Gehrels, Neil↗

NASA NTRS1993

Optical Properties of Semiconductor-Metal Composite Thin Films in the Infrared Region

Germanium:Silver (Ge:Ag) composite thin films having different concentrations of Ag, ranging from 7% to 40% have been prepared by dc co-sputtering of Ge an Ag and the films' surface morphology and optical properties have been characterized using transmission electron microscopy (TEM) and infrared spectrophotometry. It is seen that while the films containing lower concentrations of Ag have island-like morphology (i.e. Ag particles distributed in a Ge matrix), the higher metallic concentration films tend to have symmetric distribution of Ag and Ge.

Films Germanium Silver↗

NASA NTRS2006

Correcting STIS CCD Point-Source Spectra for CTE Loss

We review the on-orbit spectroscopic observations that are being used to characterize the Charge Transfer Efficiency (CTE) of the STIS CCD in spectroscopic mode. We parameterize the CTE-related loss for spectrophotometry of point sources in terms of dependencies on the brightness of the source, the background level, the signal in the PSF outside the standard extraction box, and the time of observation. Primary constraints on our correction algorithm are provided by measurements of the CTE loss rates for simulated spectra (images of a tungsten lamp taken through slits oriented along the dispersion axis) combined with estimates of CTE losses for actual spectra of spectrophotometric standard stars in the first order CCD modes. For point-source spectra at the standard reference position at the CCD center, CTE losses as large as 30% are corrected to within approx.1% RMS after application of the algorithm presented here, rendering the Poisson noise associated with the source detection itself to be the dominant contributor to the total flux calibration uncertainty.

Goudfrooij, Paul↗

NASA NTRS2006

Gamma Ray Burst Discoveries by the Swift Mission

The NASA Swift mission is an innovative new multiwavelength observatory designed to determine the origin of gamma-ray bursts and use them to probe the early Universe. Swift is now in orbit since November 20, 2004 and all hardware is performing well. A new-technology wide-field gamma-ray camera is detecting a hundred bursts per year. Sensitive narrow-field X-ray and UV/optical telescopes, built in collaboration with UK and Italian partners, are pointed at the burst location in 50-100 sec by an autonomously controlled "swift" spacecraft. For each burst, arcsec positions are determined and optical/UV/X-ray/gamma-ray spectrophotometry performed. Information is also rapidly sent to the ground to a team of more than 50 observers at telescopes around the world. The first year and a half of findings from the mission will be presented. There has been a break-through in the long-standing mystery of short GRBs; they appear to be caused by merging neutron stars. High redshift bursts have been detected leading to a better understanding of star formation rates and distant galaxy environments. A fascinating nearby burst triggered Swift and enabled the best early-time observations of an emerging coincident Type lb/c supernova. GRBs have been found with giant X-ray flares occurring in their afterglow.

Gehrels, Neil↗

NASA NTRS2007

The New Era of Swift Observations

Swift was launched in November 2004 and is performing observations of 100 GRBs per year. For each burst, arcsec positions are determined and optical/ultraviolet/gamma-ray spectrophotometry performed. Information is rapidly sent to the ground to a team of more than 50 observers at telescopes around the world. The observatory contributes in several ways to supernova research. The first is in detecting nearby GRBs that may be coincident with supernovae. The prompt Swift trigger and rapid repointing enables observations of the supernova from the time of core collapse. The observations of SN 2006aj in conjunction with GRB 060218 is an example of this capability. Swift is also performing optical, ultraviolet and X-ray observations of approx. 15 supernovae of all types per year. The talk will give an overview of the new results and discuss future prospects for Swift observations.

Gehrels, Neil↗