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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 631 records · Page 35
All sourcesNASA NTRSDOE OSTI
NASA NTRSDate not supplied

A Pipeline for Assessing the Quality of Rna-Seq Datasets in GeneLab

Transcriptome profiling by RNA sequencing (RNA-seq) is a powerful approach to identify gene expression changes in organisms exposed to unique environments such as spaceflight. One of the challenges of evaluating RNA-seq data both within and across different space-relevant studies is the ability to control for technical differences, including the use of different library preparation kits, sequencing platforms, RNA yield, and person-to-person variation. To help address this issue, the National Institute of Standards and Technology (NIST, nist.gov) initiated a consortium, at the request of industry and academia, to develop a set of controls for gene expression measurements. The result was a set of 92 unlabeled, polyadenylated transcripts that range from 250 – 2,000 nucleotides in length to mimic natural eukaryotic mRNAs. These External RNA Controls Consortium (ERCC) genes can be used in any RNA-seq experiment, by adding known concentrations of the ERCC genes to samples after RNA extraction, to offer a standard measurement for data comparison. At NASA GeneLab, we employ these controls as part of our standard operating procedures for every in-house RNA-seq study to assess the limit of detection, dynamic range, and power of differential expression analysis both within and across experiments. Here we will discuss the use, benefits, and limitations of ERCC genes and other types of controls, such as universal RNA references, to generate quality control information for RNA-seq studies conducted at GeneLab.

GeneLab↗
NASA NTRSDate not supplied

Digital Elevation Map Parametric Error Analysis Pipeline using Corresponding NAC Images

Future lunar landing systems, particularly those used to land humans on the lunar surface as part of the ARTEMIS program, will require precision navigation relative to the lunar surface. The most common way to meet these stringent navigation requirements is through terrain relative navigation (TRN), which localizes a spacecraft by comparing descent imagery with a predefined map of the surface. The accuracy achievable using TRN is limited by the accuracy of the reference Digital Elevation Map (DEM). It is therefore critical for future lunar missions that potential errors in DEMs be quantified. This paper describes one of NASA’s current efforts to develop a process for evaluating lunar DEM quality.

Chris Gnam↗
NASA NTRSDate not supplied

Automated Downlink Pipeline for Scientific Data Using TReK

ISS users generate scientific data files on orbit that require console operators to retrieve and deliver them for analysis. Automating the downlink process using TReK CFDP and DTN provides ground flight controllers and PD teams increased efficiency, reducing workload and resulting in cost savings without reduced services provided.

TReK↗