Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “Expression systems”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 631 records · Page 35
All sourcesNASA NTRSDOE OSTI
DOE OSTI2021

Biophysical characterization and a roadmap towards the NMR solution structure of G0S2, a key enzyme in non-alcoholic fatty liver disease

In the United States non-alcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease, affecting an estimated 80 to 100 million people. It occurs in every age group, but predominantly in people with risk factors such as obesity and type 2 diabetes. NAFLD is marked by fat accumulation in the liver leading to liver inflammation, which may lead to scarring and irreversible damage progressing to cirrhosis and liver failure. In animal models, genetic ablation of the protein G0S2 leads to alleviation of liver damage and insulin resistance in high fat diets. The research presented in this paper aims to aid in rational based drug design for the treatment of NAFLD by providing a pathway for a solution state NMR structure of G0S2. Here we describe the expression of G0S2 in an E. coli system from two different constructs, both of which are confirmed to be functionally active based on the ability to inhibit the activity of Adipose Triglyceride Lipase. In one of the constructs, preliminary NMR spectroscopy measurements show dominant alpha-helical characteristics as well as resonance assignments on the N-terminus of G0S2, allowing for further NMR work with this protein. Additionally, the characterization of G0S2 oligomers are outlined for both constructs, suggesting that G0S2 may defensively exist in a multimeric state to protect and potentially stabilize the small 104 amino acid protein within the cell. This information presented on the structure of G0S2 will further guide future development in the therapy for NAFLD.

59 BASIC BIOLOGICAL SCIENCES↗
NASA NTRS1972

Dynamic response of a plane-symmetrical exothermic reaction center.

?sger,An analysis of the dynamic behavior of an idealized, plane-symmetrical exothermic reaction center is presented. The conservation equations for the reaction center are combined and yield a single integral equation expressing a nonlinear transfer function of the system for which the input is provided by a given time profile of the heat released per unit mass while the output gives the pressure pulse it generates under the restriction of plane-symmetrical motion. The solution is governed by a Daumk]hler number. For a given form of the exothermic power pulse profile, the dynamic behavior of the system is completely specified in terms of only this Daumk]hler number and the heat of reaction per unit mass of the combustible medium. Specific solutions are worked out for a set of typical elementary power pulse profiles, and the practical significance of the results is illustrated by their application to the problem of transition to detonation in an explosive gas.

Meyer, J. W.↗
NASA NTRS1977

An approximate method for design and analysis of an ALOHA system

An approximate method for the design and performance prediction of a multiaccess communication system which employs the ALOHA packet-switching technique is developed, based on the use of a diffusion process approximation of an ALOHA-like system (with or without time-slotting). A simple closed-form solution for the variable Q(t), a variant of the number of backlog messages at time t, is given in terms of a few system and user parameters. Final results are expressed in terms of ordinary performance measures such as throughput and average delay. Several numerical examples are given to demonstrate the usefulness of the approximation technique developed.

Kobayashi, H.↗
NASA NTRS1978

Whistler mode noise in Jupiter's inner magnetosphere

A study is made of the amplitude and spectral extent of whistler mode noise in the inner magnetosphere of Jupiter. It is found that the 'hat-shaped' pitch angle distributions of energetic electrons (21 and 31 MeV at L=3) are consistent with those predicted in the presence of a band-limited spectrum of whistler mode noise. The equatorial maximum linear growth rate of parallel propagating whistlers are consistent with those necessary to limit the energetic electron intensities by the whistler mode instability. It is noted that the wave phase speeds before wave reflection can occur at high latitudes and that wave growth is limited to a disk-like region centered around the magnetic equator. The frequency extent of the whistler mode noise spectrum may be estimated by the range of frequencies maximally unstable to equatorial linear growth. A value is found for the spectral density of the broadband whistler mode noise necessary to balance radial diffusion of energetic electrons above the critical range, and an expression is derived for the energetic electron system response to fluctuations about the limiting flux value.

Sentman, D. D.↗
NASA NTRS1980

Temperature control simulation for a microwave transmitter cooling system

The thermal performance of a temperature control system for the antenna microwave transmitter (klystron tube) of the Deep Space Network antenna tracking system is discussed. In particular the mathematical model is presented along with the details of a computer program which is written for the system simulation and the performance parameterization. Analytical expressions are presented.

Yung, C. S.↗
NASA NTRS1982

Information science team

Concerns are expressed about the data handling aspects of system design and about enabling technology for data handling and data analysis. The status, contributing factors, critical issues, and recommendations for investigations are listed for data handling, rectification and registration, and information extraction. Potential supports to individual P.I., research tasks, systematic data system design, and to system operation. The need for an airborne spectrometer class instrument for fundamental research in high spectral and spatial resolution is indicated. Geographic information system formatting and labelling techniques, very large scale integration, and methods for providing multitype data sets must also be developed.

Billingsley, F.↗
NASA NTRS1984

A laser velocimeter system for the Ames 40- by 80-foot and 80by 120-foot wind tunnels

A unique laser velocimeter has been developed that is capable of sensing two orthogonal velocity components from a variable remote distance of 2.6 to 10 m for use in the Ames 40- by 80-Foot and 80- by 120-Foot wind tunnels, as well as in the Ames Outdoor Aerodynamic Research Facility. The system hardware, positioning instrumentation, and data acquisition equipment are described in detail. System capabilities and limitations are discussed, and expressions for systematic and statistical accuracy are developed. Direct and coupled laboratory measurements of a turbulent swirling flow are compared with measurements taken with a laser velocimeter of higher spatial resolution. Sample measurements taken in the open-circuit exhaust flow of the 80- by 120-Foot Wind Tunnel 1/50-scale model are presented.

Reinath, M. S.↗
NASA NTRS1990

A 4-node assumed-stress hybrid shell element with rotational degrees of freedom

An assumed-stress hybrid/mixed 4-node quadrilateral shell element is introduced that alleviates most of the deficiencies associated with such elements. The formulation of the element is based on the assumed-stress hybrid/mixed method using the Hellinger-Reissner variational principle. The membrane part of the element has 12 degrees of freedom including rotational or drilling degrees of freedom at the nodes. The bending part of the element also has 12 degrees of freedom. The bending part of the element uses the Reissner-Mindlin plate theory which takes into account the transverse shear contributions. The element formulation is derived from an 8-node isoparametric element. This process is accomplished by assuming quadratic variations for both in-plane and out-of-plane displacement fields and linear variations for both in-plane and out-of-plane rotation fields along the edges of the element. In addition, the degrees of freedom at midside nodes are approximated in terms of the degrees of freedom at corner nodes. During this process the rotational degrees of freedom at the corner nodes enter into the formulation of the element. The stress field are expressed in the element natural-coordinate system such that the element remains invariant with respect to node numbering.

Aminpour, Mohammad A.↗
NASA NTRS1990

The sensitivity of synthetic aperture radiometers for remote sensing applications from space

Aperture synthesis offers a means of realizing the full potential microwave remote sensing from space by helping to overcome the limitations set by antenna size. The result is a potentially lighter, more adaptable structure for applications in space. However, because the physical collecting area is reduced, the signal-to-noise ratio is also reduced and may adversely affect the radiometric sensitivity. Sensitivity is an especially critical issue for measurements from low earth orbit because the motion of the platform (about 7 km/s) limits the integration time available for forming an image. The purpose of this paper is to develop expressions for the sensitivity of remote sensing systems which use aperture synthesis. The objective is to develop basic equations general enough to be used to obtain the sensitivity of the several variations of aperture synthesis which have been proposed for sensors in space. The conventional microwave imager (a scanning total power radiometer) is treated as a special case, and the paper concludes with a comparison of three synthetic aperture configurations with the conventional imager.

Le Vine, David M.↗
NASA NTRS1991

Airborne observations of electric fields around growing and decaying cumulus clouds

Airborne electric field data were gathered in an atmospheric electrification study near Cape Canaveral, FL. A Learjet 36A was instrumented with eight electric field meters (mills) and five different particle probes. The local electric field enhancements at each field mill site were determined under lab conditions and verified using in-flight data. The overdetermined system of eight equations (one for each field mill) was solved using a weighted least squares algorithm to compute the magnitude and direction of the ambient electric field. The signal processing system allowed the measured data to be expressed in terms of earth coordinates, regardless of the attitude of the aircraft. Thus, it was possible to take maximum advantage of the Learjet's speed and maneuverability in studying the electric field structure in the vicinity of the clouds. Data gathered while circling just outside the boundary of a growing cumulus cloud show a nonsymmetric pattern of electric field strength. Field intensity grew rapidly over a period of less than 10 minutes. The observed direction of the ambient electric field vector can be explained by an ascending motion of the charge centers of a classic tripole model of a thunderstorm.

Giori, K. L.↗
NASA NTRS1990

Time-dependent treatment of scattering. II - Novel integral equation approach to quantum wave packets

The novel wave-packet propagation scheme presented is based on the time-dependent form of the Lippman-Schwinger integral equation and does not require extensive matrix inversions, thereby facilitating application to systems in which some degrees of freedom express the potential in a basis expansion. The matrix to be inverted is a function of the kinetic energy operator, and is accordingly diagonal in a Bessel function basis set. Transition amplitudes for various orbital angular momentum quantum numbers are obtainable via either Fourier transform of the amplitude density from the time to the energy domain, or the direct analysis of the scattered wave packet.

Sharafeddin, Omar A.↗
NASA NTRS1989

The sensitivity of synthetic aperture radiometers for remote sensing applications from space

Aperture synthesis offers a means of realizing the full potential of microwave remote sensing from space by helping to overcome the limitations set by antenna size. The result is a potentially lighter, more adaptable structure for applications in space. However, because the physical collecting area is reduced, the signal-to-noise ratio is reduced and may adversely affect the radiometric sensitivity. Sensitivity is an especially critical issue for measurements to be made from low earth orbit because the motion of the platform limits the integration time available for forming an image. The purpose is to develop expression for the sensitivity of remote sensing systems which use aperture synthesis. The objective is to develop basic equations general enough to be used to obtain the sensitivity of the several variations of aperture synthesis which were proposed for sensors in space. The conventional microwave imager (a scanning total power radiometer) is treated as a special case and a comparison of three synthetic aperture configurations with the conventional imager is presented.

Levine, D. M.↗
NASA NTRS1955

Theory of reversible and nonreversible cracks in solids

The Griffith crack theory is reviewed and certain shortcomings of this theory are discussed. A new description for the shape of a crack is given which takes into account the atomic structure of material. Through consideration of the total energy of the system and the shape of the crack, expressions for crack behavior are derived which are considered to remedy the defects of the Griffith theory.

PROPERTIES, MATERIALS - TENSILE↗
NASA NTRS1960

NASA Research on the Hydrodynamics of the Gaseous Vortex Reactor

The experimental and analytical results to date of a study of a two-component gaseous vortex system are presented in this paper. Analytical expressions for tangential velocity and static-pressure profiles in a turbulent vortex show good agreement with experimental data. Airflow rates from 0.075 to 0.14 pound per second and corresponding tangential velocities from 160 to 440 feet per second are correlated by turbulent Reynolds numbers from 1.95 to 2.4. An analysis of an air-bromine gas mixture in a turbulent vortex indicates that a boundary value of bromine-to-air radial velocity ratio (u(2)/u(1)) of 0.999 gives essentially no bromine buildup, while a value of 0.833 results in considerable separation. For a constant value of (u(2)/u(1))(0) the bromine buildup increases as (1) the tangential velocity increases, (2) the air-to-bromine weight-flow ratio decreases, (3) the airflow rate decreases, (4) the temperature decreases, and (5) the turbulence decreases. Analytical temperature, pressure, and tangential-velocity profiles are also presented. Preliminary experimental results indicate that the flow of an air-bromine mixture through a vortex field results in a bromine density increase to a maximum value; followed by a decrease; the air density exhibits a uniform decrease from the outer vortex radius to the exhaust-nozzle radius.

Ragsdale, Robert G.↗
NASA NTRS1999

Large Field Visualization with Demand-Driven Calculation

We present a system designed for the interactive definition and visualization of fields derived from large data sets: the Demand-Driven Visualizer (DDV). The system allows the user to write arbitrary expressions to define new fields, and then apply a variety of visualization techniques to the result. Expressions can include differential operators and numerous other built-in functions, ail of which are evaluated at specific field locations completely on demand. The payoff of following a demand-driven design philosophy throughout becomes particularly evident when working with large time-series data, where the costs of eager evaluation alternatives can be prohibitive.

Moran, Patrick J.↗
NASA NTRS2000

Parametric Modeling and Fault Tolerant Control

Fault tolerant control is considered for a nonlinear aircraft model expressed as a linear parameter-varying system. By proper parameterization of foreseeable faults, the linear parameter-varying system can include fault effects as additional varying parameters. A recently developed technique in fault effect parameter estimation allows us to assume that estimates of the fault effect parameters are available on-line. Reconfigurability is calculated for this model with respect to the loss of control effectiveness to assess the potentiality of the model to tolerate such losses prior to control design. The control design is carried out by applying a polytopic method to the aircraft model. An error bound on fault effect parameter estimation is provided, within which the Lyapunov stability of the closed-loop system is robust. Our simulation results show that as long as the fault parameter estimates are sufficiently accurate, the polytopic controller can provide satisfactory fault-tolerance.

Wu, N. Eva↗
NASA NTRS1990

The Design-To-Cost Manifold

Design-to-cost is a popular technique for controlling costs. Although qualitative techniques exist for implementing design to cost, quantitative methods are sparse. In the launch vehicle and spacecraft engineering process, the question whether to minimize mass is usually an issue. The lack of quantification in this issue leads to arguments on both sides. This paper presents a mathematical technique which both quantifies the design-to-cost process and the mass/complexity issue. Parametric cost analysis generates and applies mathematical formulas called cost estimating relationships. In their most common forms, they are continuous and differentiable. This property permits the application of the mathematics of differentiable manifolds. Although the terminology sounds formidable, the application of the techniques requires only a knowledge of linear algebra and ordinary differential equations, common subjects in undergraduate scientific and engineering curricula. When the cost c is expressed as a differentiable function of n system metrics, setting the cost c to be a constant generates an n-1 dimensional subspace of the space of system metrics such that any set of metric values in that space satisfies the constant design-to-cost criterion. This space is a differentiable manifold upon which all mathematical properties of a differentiable manifold may be applied. One important property is that an easily implemented system of ordinary differential equations exists which permits optimization of any function of the system metrics, mass for example, over the design-to-cost manifold. A dual set of equations defines the directions of maximum and minimum cost change. A simplified approximation of the PRICE H(TM) production-production cost is used to generate this set of differential equations over [mass, complexity] space. The equations are solved in closed form to obtain the one dimensional design-to-cost trade and design-for-cost spaces. Preliminary results indicate that cost is relatively insensitive to changes in mass and that the reduction of complexity, both in the manufacturing process and of the spacecraft, is dominant in reducing cost.

Dean, Edwin B.↗