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At least 631 records · Page 35

A large accessory genome and high recombination rates may influence global distribution and broad host range of the fungal plant pathogen Claviceps purpurea

Pangenome analyses are increasingly being utilized to study the evolution of eukaryotic organisms. While pangenomes can provide insight into polymorphic gene content, inferences about the ecological and adaptive potential of such organisms also need to be accompanied by additional supportive genomic analyses. In this study we constructed a pangenome of Claviceps purpurea from 24 genomes and examined the positive selection and recombination landscape of an economically important fungal organism for pharmacology and agricultural research. Together, these analyses revealed that C. purpurea has a relatively large accessory genome (~ 38%), high recombination rates (ρ = 0.044), and transposon mediated gene duplication. However, due to observations of relatively low transposable element (TE) content (8.8%) and a lack of variability in genome sizes, prolific TE expansion may be controlled by frequent recombination. We additionally identified that within the ergoline biosynthetic cluster the lpsA1 and lpsA2 were the result of a recombination event. However, the high recombination rates observed in C. purpurea may be influencing an overall trend of purifying selection across the genome. These results showcase the use of selection and recombination landscapes to identify mechanisms contributing to pangenome structure and primary factors influencing the evolution of an organism.

59 BASIC BIOLOGICAL SCIENCES↗

Ultra-High-Performance Concrete And Advanced Manufacturing Methods For Modular Construction

Small modular reactors (SMR) allow for less onsite construction, increase nuclear material security, and provide a flexible and cost-effective energy alternative. SMR can be factory-built as modular components, and shipped to desired locations for fast assembly. This project successfully developed a new class of ultra-high performance concrete (UHPC), which features a compressive strength greater than 22 ksi (150 MPa) without special treatment and self-consolidating characteristics desired for SMR modular construction. With an ultra-high strength and dense microstructure, it will facilitate rapid construction of steel plate-concrete (SC) beams and walls with thinner and lighter modules, and can withstand harsh environments and mechanical loads anticipated during the service life of nuclear power plants. In addition, the self-consolidating characteristics are crucial for the fast construction and assembly of SC modules with reduced labor costs and improved quality. Following the UHPC material development, the capacity of producing self-consolidating UHPC in mass quantities was investigated and compared to accepted self-consolidating concrete standards. With slightly adjusted mixing procedure using large-scale gravity-based mixers (compared with small-scale force-based mixer), the self-consolidating UHPC has been successfully processed at six cubic yards; the product met both minimum compressive strength requirements and self-consolidating concrete standards. Steel plate-UHPC beams (15 ft. long, 12 in. wide and 16 in. deep) and wall panels (40 in. X 40 in. X 3 in.) were then constructed using the self-consolidating UHPC without any external vibration. Quality control guidelines for producing UHPC in large scale were developed. When the concrete is replaced by UHPC in a steel plate concrete (SC) beam, it is critical to evaluate its structural behavior with both flexure and shear-governed failure modes. In recent years, SC has been widely used for buildings and nuclear containment structures to resist lateral forces induced by severe earthquakes and heavy winds. SC modules have good potential for SMR because of their cost-effectiveness and reduced construction time. However, the minimum shear reinforcement (i.e. cross tie) ratio needs to be determined for the steel plate-UHPC (S-UHPC) beams to exhibit a ductile failure mode. In this project, S-UHPC beams were designed and constructed. The beams were tested to evaluate structural capacity and identify the minimum cross ties ratios. In addition, as the bond between UHPC and steel plate is essential for ensuring structural integrity under shear and flexure, it was measured and examined in this project through digital image correlation system and smart piezoelectric aggregate sensors. Large-scale testing and finite element simulation were also performed on S-UHPC wall panels. New bond slip-based constitutive models of steel plate were developed for S-UHPC, which were used in finite element analysis program to predict S-UHPC behavior under shear. The results were well validated through experimental data. The long-term durability of UHPC were established in this project. UHPC specimens were tested under free shrinkage, restrained shrinkage, elevated temperature, water permeation, chloride diffusion, corrosion, and alkali silica reaction. UHPC has demonstrated significantly improved durability compared with control concrete specimens. This research led to a new generation of steel plate-UHPC modules for SMR that can provide large benefits to the electric power industry. Taking advantage of the high strength and durability of UHPC, their modularity and ease of assembly can address the high cost barriers of typical nuclear power plants.

42 ENGINEERING↗

Experimental Validation of a High-Temperature Test Facility for Future Additive Manufactured Supercritical Carbon Dioxide Turbine Testing

For next-generation power plants to achieve high cycle efficiencies consistent with the Department of Energy's 65% efficiency target, turbomachinery capable of operating within high-temperature power cycles must be demonstrated. Pairing additively manufactured superalloy turbines with the supercritical carbon dioxide (sCO 2 ) power cycle could enable turbine inlet temperatures approaching 1300 °C while providing flexibility in turbine cooling strategies. Development of test facilities to characterize and validate such systems is crucial. In this study, a turbine test facility capable of achieving inlet conditions of 800 °C, 11 MPa, and 0.43 kg/s while accommodating complex auxiliary cooling flow requirements has been designed and constructed to support future testing of a Haynes 282 additively manufactured 30 kW turbine-generator system with advanced cooling channels. This facility enables characterization of aerodynamic performance, leakage, and windage losses. Details of the facility's construction and operation are presented, along with experimental validation tests using an orifice as an expansion device in place of the turbine. These tests confirm that the facility can reach the required conditions, distinguish regions of achievable steady and pseudo-steady conditions, and identify the heater power required for each point in the upcoming turbine campaign. The campaign confirmed that ISO 5167-2 can reliably predict orifice mass flow rates in extreme supercritical carbon dioxide conditions with deviations of 0.5–7.5%.

42 ENGINEERING↗

Additive manufacturing integrated energy—enabling innovative solutions for buildings of the future

Here, the AMIE (Additive Manufacturing Integrated Energy) demonstration utilized 3D printing as an enabling technology in the pursuit of construction methods that use less material, create less waste, and require less energy to build and operate. It was developed by Oak Ridge National Laboratory (ORNL) in collaboration with the Governor's Chair for Energy and Urbanism, a research partnership of the University of Tennessee (UT) and ORNL led by Skidmore, Owings & Merrill LLP (SOM), AMIE embodies a suite of innovations demonstrating a transformative future for designing, constructing and operating buildings. Subsequent, blind UT College of Architecture and Design studios taught in collaboration with SOM professionals also explored forms and shapes based on biological systems that naturally integrate structure and enclosure. AMIE, a compact micro-dwelling developed by ORNL research scientists and SOM designers, incorporates next-generation modified atmosphere insulation, self-shading windows, and the ability to produce, store and share solar power with a paired hybrid vehicle. It establishes for the first time, a platform for investigating solutions integrating the energy systems in buildings, vehicles, and the power grid. The project was built with broad-based support from local industry and national material suppliers. Designed and constructed in a span of only nine months, AMIE 1.0 serves as an example of the rapid innovation that can be accomplished when research, design, academic and industrial partners work in collaboration toward the common goal of a more sustainable and resilient built environment.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

A Review of Extra-Terrestrial Regolith Excavation Concepts and Prototypes

Regolith is present on many extra-terrestrial bodies, and the crushed rock material it is made of contains many of the resources that are enabling for In-Situ Resource Utilization (ISRU). When extracted these resources can be used to provide consumables such as rocket propellant, human life support, working fluids and gases for industrial processes and feedstocks for manufacturing. In addition, the regolith can also be very beneficial for construction purposes as an aggregate which can be used for construction materials and shielding for radiation protection and micrometeorite impact. Binders for regolith concrete may also be made from geopolymers that may be in the regolith. The regolith can be melted and drawn out into glass fibers and used as reinforcements in a metal, polymer, or concrete matrix. In addition, there is tremendous scientific and geological knowledge that can only be obtained by studying samples of the regolith. However, none of these valuable activities can proceed without first acquiring the regolith granular material with some type of excavation device and method. Excavation is in the critical path of many workflows that will make up the capabilities required to establish a human and robotic presence in our solar system. While scientific in-situ sampling of regolith in small quantities has been achieved since the dawn of the space age in the 1960’s, large scale excavation for mining and construction on extra-terrestrial bodies has only been contemplated for many decades in works of scientific fact and also in fictional stories, but serious development and prototyping of excavation technologies for use in reduced gravity space environments was only started in the late 1990’s.This paper will review and document the evolution of extra-terrestrial excavation concepts and prototypes based on the available literature and the personal experience of the author who has been working on regolith excavation technology development since 1998.

ISRU↗

A Review of Extra-Terrestrial Regolith Excavation Concepts and Prototypes

Regolith is present on many extra-terrestrial bodies, and the crushed rock material it is made of contains many of the resources that are enabling for In-Situ Resource Utilization (ISRU). When extracted, these resources can be used to provide consumables such as rocket propellant, human life support, working fluids and gases for industrial processes and feedstocks for manufacturing. In addition, the regolith can also be very beneficial for construction purposes as an aggregate which can be used for construction materials and shielding for radiation protection and micrometeorite impact. Binders for regolith concrete may also be made from geopolymers that may be in the regolith. The regolith can be melted and drawn out into glass fibers and used as reinforcements in a metal, polymer, or concrete matrix. In addition, there is tremendous scientific and geological knowledge that can only be obtained by studying samples of the regolith. However, none of these valuable activities can proceed without first acquiring the regolith granular material with some type of excavation device and method. Excavation is in the critical path of many workflows that will make up the capabilities required to establish a human and robotic presence in our solar system. While scientific in-situ sampling of regolith in small quantities has been achieved since the dawn of the space age in the 1960’s, large scale excavation for mining and construction on extra-terrestrial bodies has only been contemplated, for many decades, but serious development and prototyping of excavation technologies for use in reduced gravity space environments was only started in the late 1990’s. This paper will review and document the evolution of extra-terrestrial excavation concepts and prototypes based on the available literature and the personal experience of the author who has been working on regolith excavation technology development since 1998.

Regolith↗

A Review of Extra-Terrestrial Regolith Excavation Concepts and Prototype

Regolith is present on many extra-terrestrial bodies, and the crushed rock material it is made of contains many of the resources that are enabling for In-Situ Resource Utilization (ISRU). When extracted, these resources can be used to provide consumables such as rocket propellant, human life support, working fluids and gases for industrial processes and feedstocks for manufacturing. In addition, the regolith can also be very beneficial for construction purposes as an aggregate which can be used for construction materials and shielding for radiation protection and micrometeorite impact. Binders for regolith concrete may also be made from geopolymers that may be in the regolith. The regolith can be melted and drawn out into glass fibers and used as reinforcements in a metal, polymer, or concrete matrix. In addition, there is tremendous scientific and geological knowledge that can only be obtained by studying samples of the regolith. However, none of these valuable activities can proceed without first acquiring the regolith granular material with some type of excavation device and method. Excavation is in the critical path of many workflows that will make up the capabilities required to establish a human and robotic presence in our solar system. While scientific in-situ sampling of regolith in small quantities has been achieved since the dawn of the space age in the 1960’s, large scale excavation for mining and construction on extra-terrestrial bodies has only been contemplated, for many decades, but serious development and prototyping of excavation technologies for use in reduced gravity space environments was only started in the late 1990’s. This paper will review and document the evolution of extra-terrestrial excavation concepts and prototypes based on the available literature and the personal experience of the author who has been working on regolith excavation technology development since 1998.

Regolith↗

Generating parity relations for detecting and identifying control system component failures

The monitoring of control system sensors and actuators for failures is presently undertaken by means of an exceptionally simple form of generalized parity relations based on a discrete-time model of the dynamics of linear, time-invariant systems. These generalized parity relations are constructed by recourse to a transfer matrix-description of the system that is additionally useful in the interpretation of their properties. Attention is given to a novel method for constructing the parity relation of minimum length that depends on the output of only a single sensor.

Vander Velder, Wallace E.↗

HESTIA Commodities Exchange Pallet and Sounding Rocket Test Stand

During my Spring 2016 internship, my two major contributions were the design of the Commodities Exchange Pallet and the design of a test stand for a 100 pounds-thrust sounding rocket. The Commodities Exchange Pallet is a prototype developed for the Human Exploration Spacecraft Testbed for Integration and Advancement (HESTIA) program. Under the HESTIA initiative the Commodities Exchange Pallet was developed as a method for demonstrating multi-system integration thru the transportation of In-Situ Resource Utilization produced oxygen and water to a human habitat. Ultimately, this prototype's performance will allow for future evaluation of integration, which may lead to the development of a flight capable pallet for future deep-space exploration missions. For HESTIA, my main task was to design the Commodities Exchange Pallet system to be used for completing an integration demonstration. Under the guidance of my mentor, I designed, both, the structural frame and fluid delivery system for the commodities pallet. The fluid delivery system includes a liquid-oxygen to gaseous-oxygen system, a water delivery system, and a carbon-dioxide compressors system. The structural frame is designed to meet safety and transportation requirements, as well as the ability to interface with the ER division's Portable Utility Pallet. The commodities pallet structure also includes independent instrumentation oxygen/water panels for operation and system monitoring. My major accomplishments for the commodities exchange pallet were the completion of the fluid delivery systems and the structural frame designs. In addition, parts selection was completed in order to expedite construction of the prototype, scheduled to begin in May of 2016. Once the commodities pallet is assembled and tested it is expected to complete a fully integrated transfer demonstration with the ISRU unit and the Environmental Control and Life Support System test chamber in September of 2016. In addition to the development of the Commodities Exchange Pallet, I also assisted in preparation for testing the upper stage of a sounding rocket developed as a Center Innovation Fund project. The main objective of this project is to demonstrate the integration between a propulsion system and a solid oxide fuel cell (SOFC). The upper stage and SOFC are scheduled to complete an integrated test in August of 2016. As part of preparation for scheduled testing, I was responsible for designing the upper stage's test stand/support structure and main engine plume deflector to be used during hot-fire testing (fig. 3). The structural components of the test stand need to meet safety requirements for operation of the propulsion system, which consist of a 100 pounds-thrust main engine and two 15 pounds-thrust reaction control thrusters. My main accomplishment for this project was the completion of the design and the parts selection for construction of the structure, scheduled to begin late April of 2016.

Chaparro, Javier↗

Selection of an Alternate Biocide for the ISS Internal Thermal Control System Coolant, Phase 2

The ISS (International Space Station) ITCS (Internal Thermal Control System) includes two internal coolant loops that utilize an aqueous based coolant for heat transfer. A silver salt biocide had previously been utilized as an additive in the coolant formulation to control the growth and proliferation of microorganisms within the coolant loops. Ground-based and in-flight testing demonstrated that the silver salt was rapidly depleted, and did not act as an effective long-term biocide. Efforts to select an optimal alternate biocide for the ITCS coolant application have been underway and are now in the final stages. An extensive evaluation of biocides was conducted to down-select to several candidates for test trials and was reported on previously. Criteria for that down-select included: the need for safe, non-intrusive implementation and operation in a functioning system; the ability to control existing planktonic and biofilm residing microorganisms; a negligible impact on system-wetted materials of construction; and a negligible reactivity with existing coolant additives. Candidate testing to provide data for the selection of an optimal alternate biocide is now in the final stages. That testing has included rapid biocide effectiveness screening using Biolog MT2 plates to determine minimum inhibitory concentration (amount that will inhibit visible growth of microorganisms), time kill studies to determine the exposure time required to completely eliminate organism growth, materials compatibility exposure evaluations, coolant compatibility studies, and bench-top simulated coolant testing. This paper reports the current status of the effort to select an alternate biocide for the ISS ITCS coolant. The results of various test results to select the optimal candidate are presented.

Wilson, Mark E.↗

An Experimental Evaluation of Oil Pumping Rings

The design and construction of a reciprocating test vehicle to be used in evaluating hydrodynamic oil pumping rings are discussed. In addition, experimental test data are presented for three pumping ring designs that were constructed from Tin-Based Babbitt (SAE 11), Bearing Bronze (SAE 660), and Mechanical Carbon Graphite (Union Carbide Grade CNF-J). Data of pumped flow rate versus delivered pressure, as well as friction loss, are reported for the following conditions: frequencies of 10, 35 and 45 Hz; strokes of 25.4 mm (1.00 in.), 38.1 mm (1.50 in.) and 50.8 mm (2.00 in.) oil inlet temperature of 49 degrees (120 degrees); and pumping ring close-in pressures of 10.3 MPa (1500 lb/square inch. A 20W40 automotive oil was used for all tests. The maximum delivered pressure was 11 MPa (1600 lb/square inch. An analysis of hydrodynamic oil pumping rings was performed and the results of the analysis were compared to measured test data.

Eusepi, M. W.↗

Biophysical characterization and a roadmap towards the NMR solution structure of G0S2, a key enzyme in non-alcoholic fatty liver disease

In the United States non-alcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease, affecting an estimated 80 to 100 million people. It occurs in every age group, but predominantly in people with risk factors such as obesity and type 2 diabetes. NAFLD is marked by fat accumulation in the liver leading to liver inflammation, which may lead to scarring and irreversible damage progressing to cirrhosis and liver failure. In animal models, genetic ablation of the protein G0S2 leads to alleviation of liver damage and insulin resistance in high fat diets. The research presented in this paper aims to aid in rational based drug design for the treatment of NAFLD by providing a pathway for a solution state NMR structure of G0S2. Here we describe the expression of G0S2 in an E. coli system from two different constructs, both of which are confirmed to be functionally active based on the ability to inhibit the activity of Adipose Triglyceride Lipase. In one of the constructs, preliminary NMR spectroscopy measurements show dominant alpha-helical characteristics as well as resonance assignments on the N-terminus of G0S2, allowing for further NMR work with this protein. Additionally, the characterization of G0S2 oligomers are outlined for both constructs, suggesting that G0S2 may defensively exist in a multimeric state to protect and potentially stabilize the small 104 amino acid protein within the cell. This information presented on the structure of G0S2 will further guide future development in the therapy for NAFLD.

59 BASIC BIOLOGICAL SCIENCES↗

Contamination control and assay results for the Majorana Demonstrator ultra clean components

The Majorana Demonstrator is a neutrinoless double beta decay experiment utilizing enriched Ge-76 detectors in 2 separate modules inside of a common solid shield at the Sanford Underground Research Facility. The Demonstrator has utilized world leading assay sensitivities to develop clean materials and processes for producing ultra-pure copper and plastic components. This experiment is now operating, and initial data provide new insights into the success of cleaning and processing. Post production copper assays after the completion of Module 1 showed an increase in U and Th contamination in finished parts compared to starting bulk material. A revised cleaning method and additional round of surface contamination studies prior to Module 2 construction have provided evidence that more rigorous process control can reduce surface contamination. In conclusion, this article describes the assay results and discuss further studies to take advantage of assay capabilities for the purpose of maintaining ultra clean fabrication and process design.

36 MATERIALS SCIENCE↗

Degree-preserving graph dynamics: a versatile process to construct random networks

Real-world networks evolve over time via the addition or removal of vertices and edges. In current network evolution models, vertex degree varies or grows arbitrarily. A recently introduced degree-preserving network growth (DPG) family of models preserves vertex degree, resulting in structures significantly different from and more diverse than previous models. Despite its degree preserving property, the DPG model is able to replicate the output of several well-known real-world network growth models. Simulations showed that many real-world networks can also be constructed from small seed graphs via the DPG process. Here, we start the development of a rigorous mathematical theory underlying the DPG family of network growth models. We prove that the degree sequence of the output of some of the well-known, real-world network growth models can be reconstructed via the DPG process, using proper parametrization. We also show that the general problem of deciding whether a simple graph can be obtained via the DPG process from a small seed (DPG feasibility) is, however, NP-complete. In conclusion, it is an intriguing open problem to uncover whether there is a structural reason behind the DPG-constructability of real-world networks.

97 MATHEMATICS AND COMPUTING↗

Polynomial Chaos Surrogate Construction for Random Fields with Parametric Uncertainty

Engineering and applied science rely on computational experiments to rigorously study physical systems. The mathematical models used to probe these systems are highly complex, and sampling-intensive studies often require prohibitively many simulations for acceptable accuracy. Surrogate models provide a means of circumventing the high computational expense of sampling such complex models. In particular, polynomial chaos expansions (PCEs) have been successfully used for uncertainty quantification studies of deterministic models where the dominant source of uncertainty is parametric. We discuss an extension to conventional PCE surrogate modeling to enable surrogate construction for stochastic computational models that have intrinsic noise in addition to parametric uncertainty. We develop a PCE surrogate on a joint space of intrinsic and parametric uncertainty, enabled by Rosenblatt transformations, which are evaluated via kernel density estimation of the associated conditional cumulative distributions. Furthermore, we extend the construction to random field data via the Karhunen–Loève expansion. We then take advantage of closed-form solutions for computing PCE Sobol indices to perform a global sensitivity analysis of the model which quantifies the intrinsic noise contribution to the overall model output variance. Additionally, the resulting joint PCE is generative in the sense that it allows generating random realizations at any input parameter setting that are statistically approximately equivalent to realizations from the underlying stochastic model. The method is demonstrated on a chemical catalysis example model and a synthetic example controlled by a parameter that enables a switch from unimodal to bimodal response distributions.

97 MATHEMATICS AND COMPUTING↗

On the Additive Structure and Asymptotics of Codimensions c{sub n} in the Algebra F{sup (5)}

In this paper, we investigate the additive structure of the algebra F{sup (5)}, i.e., a relatively free, associative, countably-generated algebra with the identity [x{sub 1}, . . . , x{sub 5}] = 0 over an infinite field of characteristic ≠2, 3. We study the space of proper multilinear polynomials in this algebra and means of basis construction in one of its basic subspaces. As an additional result, we obtain estimations of codimensions c{sub n} = dimP{sub n}/P{sub n}∩ T{sup (5)}, where P{sub n} is the space of multilinear polynomials of degree n in F{sup (5)} and T{sup (5)} is the T-ideal generated by the long commutator [x{sub 1}, . . . , x{sub 5}].

97 MATHEMATICS AND COMPUTING↗

Aviation Safety Modeling and Simulation (ASMM) Propulsion Fleet Modeling: A Tool for Semi-Automatic Construction of CORBA-based Applications from Legacy Fortran Programs

Within NASA's Aviation Safety Program, NASA GRC participates in the Modeling and Simulation Project called ASMM. NASA GRC s focus is to characterize the propulsion systems performance from a fleet management and maintenance perspective by modeling and through simulation predict the characteristics of two classes of commercial engines (CFM56 and GE90). In prior years, the High Performance Computing and Communication (HPCC) program funded, NASA Glenn in developing a large scale, detailed simulations for the analysis and design of aircraft engines called the Numerical Propulsion System Simulation (NPSS). Three major aspects of this modeling included the integration of different engine components, coupling of multiple disciplines, and engine component zooming at appropriate level fidelity, require relatively tight coupling of different analysis codes. Most of these codes in aerodynamics and solid mechanics are written in Fortran. Refitting these legacy Fortran codes with distributed objects can increase these codes reusability. Aviation Safety s modeling and simulation use in characterizing fleet management has similar needs. The modeling and simulation of these propulsion systems use existing Fortran and C codes that are instrumental in determining the performance of the fleet. The research centers on building a CORBA-based development environment for programmers to easily wrap and couple legacy Fortran codes. This environment consists of a C++ wrapper library to hide the details of CORBA and an efficient remote variable scheme to facilitate data exchange between the client and the server model. Additionally, a Web Service model should also be constructed for evaluation of this technology s use over the next two- three years.

Sang, Janche↗

Data-Parallel Algorithm for Contour Tree Construction

The goal of this project is to develop algorithms for additional visualization and analysis filters in order to expand the functionality of the VTK-m toolkit to support less critical but commonly used operators.

97 MATHEMATICS AND COMPUTING↗