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All sources NASA NTRS DOE OSTI

DOE OSTI2018

Soil evaporation and organic matter turnover in the Sub-Taiga and Forest-Steppe of southwest Siberia

Southwest Siberia encompasses the forest-steppe and sub-taiga climatic zones and has historically been utilized for agriculture. Coinciding with predicted changes in climate for the region is the pressure of agricultural development; however, a characterization of the soil water and carbon dynamics is lacking. We assessed current soil water properties and soil organic carbon turnover in forests and grasslands for two sites that span the forest steppe and sub-taiga bioclimatic zones. Soil evaporation was 0.62±0.17mm d-1 (mean±standard error) in grasslands and 0.45±0.08mm d-1 in the forests of the forest-steppe site. Evaporation at the sub-taiga site was 1.80±1.70mm d-1 in grasslands and 0.96±0.05mm d-1 in forest plots. Evaporation was significantly greater at the sub-taiga site than the forest-steppe site. The density of fine roots explained the soil water isotopic patterns between vegetation types and sites. We found soil organic matter turnover to be three times faster in the sub-taiga site than in the forest-steppe site. Our results show that while climate factors, in particular snow levels, between the two sites are drivers for water and carbon cycles, site level hydrology, soil characteristics, and vegetation directly interact to influence the water and carbon dynamics.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2019

Application of Al-Cu-W-Ta graded density impactors in dynamic ramp compression experiments

Graded density impactors (GDIs) are utilized to dynamically compress materials to extreme conditions. Two modifications to a previously developed Mg-Cu-W GDI are made in this work before using it in a dynamic compression experiment: Mg is replaced with Al and a Ta disk is glued to the back. The Mg phase is replaced by Al because FCC Al remains solid to higher pressure along its Hugoniot compared to Mg. The addition of the Ta disk creates a constant particle velocity regime and facilitates a definition of peak pressure states. Microstructure analysis, profilometry, and ultrasonic C-scans of the Al-Cu-W GDI all confirm excellent uniformity. We evaluated signal variation in the radial direction of a dynamically compressed Al-LiF bilayer target to evaluate the contribution of spatial nonuniformity to errors. Velocity traces from five photon Doppler velocimetry (PDV) probes located at different radial distances from the center of the target varied at most by 1.1% with a root mean square of 0.3% during the compression ramp, demonstrating low PDV measurement error over a relatively large experimental area. The experimental PDV data additionally agrees well with 1D simulations that use inputs from predictive characterization models developed for the material properties resulting from tape casting, laminating, and powder consolidation processes. Low measurement error during quasi-isentropic compression, leading to better precision, promises a robust platform to reach extreme compression and low-temperature recovery states and facilitates discovery via synthesis, quenching, and preservation of new high-pressure phases.

36 MATERIALS SCIENCE↗

DOE OSTI2022

FATES crown damage simulation outputs 2022

This dataset contains outputs from the Functionally Assembled Terrestrial Ecosystem Simulator (FATES) and accompanies the paper "Needham, J.F., Arellano, A., Davies, S.J., Fisher, R.A., Hammer, V., Knox, R., Mitre, D., Muller-Landau, H.C., Zuleta, D., Koven, C.D. Tree crown damage and its effects on forest carbon cycling in a tropical forest, 2022, Global Change Biology". Data are unprocessed netcdf file outputs from simulations that were run to test the effect of a new crown damage module in FATES. Specifically, this data package contains a sensitivity analysis to the carbon cushion parameter damage_Ccushion_ensemble_e1b5bd9_bf013ef_2021-09-02.h0.ensemble.sofar.nc, a sensitivity analysis to the root nitrogen stoichiometry parameter damage_Nstoich_ensemble_e1b5bd9_bf013ef_2021-09-02.h0.ensemble.sofar.nc, a sensitivity analysis to parameters controlling crown damage and recovery damage_recovery_ensemble_e1b5bd9_354f0b0_2021-09-02.h0.ensemble.sofar.nc, and a sensitivity analysis to the number of crown damage bins elm_fates_bci_*_damagebins.Eac53ccb80b-F8f994c29.2022-04-19.elm.h0.fullrun.nc. This data package also contains a high root nitrogen configuration of FATES, including both a control, and a crown damage simulation high_root_N_control_e1b5bd9_354f0b0_2021-09-02.clm2.h0.fullrun.nc and high_root_N_damage_e1b5bd9_354f0b0_2021-09-02.clm2.h0.fullrun.nc. There is an analogous low root nitrogen configuration of FATES, including a control, low_root_N_control_e1b5bd9_bf013ef_2021-09-02.clm2.h0.fullrun.nc a damage only simulation low_root_N_damageonly_e1b5bd9_bf013ef_2021-09-02.clm2.h0.fullrun.nc, a damage plus mortality simulation low_root_N_damage_mort_e1b5bd9_bf013ef_2021-09-02.clm2.h0.fullrun.nc, and a mortality only simulation low_root_N_mort_only_e1b5bd9_ef845c8_2021-09-02.clm2.h0.fullrun.nc. Finally, there is a two PFT simulation in which we test the effect of recovery on competitive dynamics, low_root_N_damage_two_pfts_stoichastic_e1b5bd9_bf013ef_2021-09-10.clm2.h0.fullrun.nc. These simulations test the effect of representing crown damage in FATES, compared with simulations that have an equivalent increase in mortality. Jupyter notebooks to analyse these files can be found at https://github.com/JessicaNeedham/Needham_etal_GCB_2022_FATES_crown_damage.

54 ENVIRONMENTAL SCIENCES↗

NASA NTRS2004

An Evaluation of Quantitative Methods of Determining the Degree of Melting Experienced by a Chondrule

Many workers have considered the degree to which partial melting occurred in chondrules they have studied, and this has led to attempts to find reliable methods of determining the degree of melting. At least two quantitative methods have been used in the literature: a convolution index (CVI), which is a ratio of the perimeter of the chondrule as seen in thin section divided by the perimeter of a circle with the same area as the chondrule, and nominal grain size (NGS), which is the inverse square root of the number density of olivines and pyroxenes in a chondrule (again, as seen in thin section). We have evaluated both nominal grain size and convolution index as melting indicators. Nominal grain size was measured on the results of a set of dynamic crystallization experiments previously described, where aliquots of LEW97008(L3.4) were heated to peak temperatures of 1250, 1350, 1370, and 1450 C, representing varying degrees of partial melting of the starting material. Nominal grain size numbers should correlate with peak temperature (and therefore degree of partial melting) if it is a good melting indicator. The convolution index is not directly testable with these experiments because the experiments do not actually create chondrules (and therefore they have no outline on which to measure a CVI). Thus we had no means to directly test how well the CVI predicted different degrees of melting. Therefore, we discuss the use of the CVI measurement and support the discussion with X-ray Computed Tomography (CT) data.

Nettles, J. W.↗

NASA NTRS1999

Phase-Shifted Laser Feedback Interferometry

Phase-shifted, laser feedback interferometry is a new diagnostic tool developed at the NASA Lewis Research Center under the Advanced Technology Development (ATD) Program directed by NASA Headquarters Microgravity Research Division. It combines the principles of phase-shifting interferometry (PSI) and laser-feedback interferometry (LFI) to produce an instrument that can quantify both optical path length changes and sample reflectivity variations. In a homogenous medium, the optical path length between two points is the product of the index of refraction and the geometric distance between the two points. LFI differs from other forms of interferometry by using the laser as both the source and the phase detector. In LFI, coherent feedback of the incident light either reflected directly from a surface or reflected after transmission through a region of interest will modulate the output intensity of the laser. The combination of PSI and LFI has produced a robust instrument, based on a low-power helium-neon (HeNe) gas laser, with a high dynamic range that can be used to measure either static or oscillatory changes of the optical path length. Small changes in optical path length are limited by the fraction of a fringe that can be measured; we can measure nonoscillatory changes with a root mean square (rms) error of the wavelength/1000 without averaging.

Ovryn, Benjie↗

NASA NTRS1983

Magnetic fields and activity of the sun and stars - An overview

Recent work on the observation and theory of solar and stellar magnetic field activity and its relation to stellar activity is reviewed, emphasizing those aspects relevant to the problem of activity of red dwarf stars. New observational facts relevant to understanding the root cause of stellar surface activity are summarized and theoretical questions concerning the underlying physical basis for the observed correlations between stellar activity, rotation, and magnetic fields are addressed. These include dyanamo theory and the rotation-activity connection as well as flux tube dynamics and plasma heating.

Rosner, R.↗

NASA NTRS1987

Slewing control experiment for a flexible panel

Technology areas are identified in which better analytical and/or experimental methods are needed to adequately and accurately control the dynamic responses of multibody space platforms such as the space station. A generic space station solar panel is used to experimentally evaluate current control technologies. Active suppression of solar panel vibrations induced by large angle maneuvers is studied with a torque actuator at the root of the solar panel. These active suppression tests will identify the hardware requirements and adequacy of various controller designs.

Juang, Jer-Nan↗

DOE OSTI2022

Effects of Vegetation on Fluxes of Nitric Oxide, Nitrogen Dioxide, and Nitrous Oxide in a Mixed Deciduous Forest Clearing

Atmosphere-terrestrial exchange of nitrogen oxides, such as nitric oxide (NO), nitrogen dioxide (NO2) and nitrous oxide (N2O), plays an important role in controlling atmospheric composition and the radiative forcing of Earth’s atmosphere. In this study, the impact of vegetation ground cover on NOx (NOx = NO + NO2) and N2O fluxes in a deciduous forest clearing was studied using dynamic flow chambers located on three experimental plots with differing vegetation ground cover. Microbial emissions of NO and N2O were observed throughout the measurement period, while NO2 was exclusively deposited. Vegetation had no observable impact on NO2 deposition. Under dry conditions, total measured nitrogen emissions were numerically similar across all plots, although the N2O:NO ratio was higher for plots containing plants. Vegetation elevates soil water content through enhanced water retention, a condition that favors denitrification and more N2O production, relative to NO. Two rain events induced pulses of NO and N2O that were higher for bare soil compared to the vegetated plots. Lower NO and N2O emissions in plant-containing chambers stemmed from enhanced root uptake of N following rewetting, which diminished the amount of N substrate available for microbial NO and N2O production. The sensitivity of soil N emissions to vegetation ground cover highlights the need for more studies that investigate this effect over a wide range of ecosystems and to include vegetation effects in future parameterizations of soil NOx and N2O used in atmospheric models. This dataset includes processed data collected from 4 dynamic flux chambers and 17 sensors. Data includes ambient gas measurements made 1 m from the surface and soil flux measurements of NO, NO2, N2O, ozone (O3) and carbon dioxide (CO2). Also included is a suite of meteorological (air temperature, humidity, pressure) and soil parameters (water filled pore space, soil temperature). Refer to the readme for more information about each of the data sets.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2019

Heat current fluctuations and anomalous transport in low-dimensional carbon lattices

Molecular dynamics simulations and methods of importance sampling are used to study the heat transport of low-dimensional carbon lattices. For both carbon nanotubes and graphene sheets, heat transport is found to be anomalous, violating Fourier’s law of conduction with a system size dependent thermal conductivity and concomitant nonlinear temperature profiles. For carbon nanotubes, the thermal conductivity is found to increase as the square root of the length of the nanotube, while for graphene sheets the thermal conductivity is found to increase as the logarithm of the length of the sheet over the system sizes considered. Furthermore, the particular length dependence and nonlinear temperature profiles place carbon lattices into a universality class with nonlinear lattice models, and suggest that heat transport through carbon nanostructures is better described by a Levy walk rather than simple diffusion.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

DOE OSTI2023

The ecosystem wilting point defines drought response and recovery of a Quercus‐Carya forest

Abstract Soil and atmospheric droughts increasingly threaten plant survival and productivity around the world. Yet, conceptual gaps constrain our ability to predict ecosystem‐scale drought impacts under climate change. Here, we introduce the ecosystem wilting point (Ψ EWP ), a property that integrates the drought response of an ecosystem's plant community across the soil–plant–atmosphere continuum. Specifically, Ψ EWP defines a threshold below which the capacity of the root system to extract soil water and the ability of the leaves to maintain stomatal function are strongly diminished. We combined ecosystem flux and leaf water potential measurements to derive the Ψ EWP of a Quercus‐Carya forest from an “ecosystem pressure–volume (PV) curve,” which is analogous to the tissue‐level technique. When community predawn leaf water potential (Ψ pd ) was above Ψ EWP (=−2.0 MPa), the forest was highly responsive to environmental dynamics. When Ψ pd fell below Ψ EWP , the forest became insensitive to environmental variation and was a net source of carbon dioxide for nearly 2 months. Thus, Ψ EWP is a threshold defining marked shifts in ecosystem functional state. Though there was rainfall‐induced recovery of ecosystem gas exchange following soaking rains, a legacy of structural and physiological damage inhibited canopy photosynthetic capacity. Although over 16 growing seasons, only 10% of Ψ pd observations fell below Ψ EWP , the forest is commonly only 2–4 weeks of intense drought away from reaching Ψ EWP , and thus highly reliant on frequent rainfall to replenish the soil water supply. We propose, based on a bottom‐up analysis of root density profiles and soil moisture characteristic curves, that soil water acquisition capacity is the major determinant of Ψ EWP , and species in an ecosystem require compatible leaf‐level traits such as turgor loss point so that leaf wilting is coordinated with the inability to extract further water from the soil.

54 ENVIRONMENTAL SCIENCES↗

DOE OSTI2020

A Hybrid-Learning Algorithm for Online Dynamic State Estimation in Multimachine Power Systems

With the increasing penetration of distributed generators in the smart grids, having knowledge of rapid real-time electromechanical dynamic states has become crucial to system stability control. Conventional Supervisory Control and Data Acquisition (SCADA)-based dynamic state estimation (DSE) techniques are limited by the slow sampling rates, while the emerging phasor measurement units (PMUs) technology enables rapid real-time measurements at network nodes. Using generator bus terminal voltages, we propose a hybrid-learning DSE (HL-DSE) algorithm to estimate the synchronous machine rotor angle and speed in real time. The HL-DSE takes the power system model into account and trains neuroestimators with real-time data in an online manner. Compared with traditional DSE methods, the HL-DSE overcomes limitations by using a data-driven approach in conjunction with the physical power system model. The time efficiency, accuracy, convergence, and robustness of the proposed algorithm are tested under noises and fault conditions in both small- and large-scale test systems. Simulation results show that the proposed HL-DSE is much more computationally efficient than widely used Kalman filter (KF)-based methods while maintaining comparable accuracy and robustness. In particular, HL-DSE is over 100 times faster than square-root unscented KF (SR-UKF) and 80 times faster than extended KF (EKF). The advantages and challenges of the HL-DSE are also discussed.

24 POWER TRANSMISSION AND DISTRIBUTION↗

NASA NTRS2005

The Role of Structural Dynamics and Testing in the Shuttle Flowliner Crack Investigation

During a normal inspection of the main propulsion system at Kennedy Space Center, small cracks were noticed near a slotted region of a gimbal joint flowliner located just upstream from one of the Space Shuttle Main Engines (SSME). These small cracks sparked an investigation of the entire Space Shuttle fleet main propulsion feedlines. The investigation was initiated to determine the cause of the small cracks and a repair method that would be needed to return the Shuttle fleet back to operation safely. The cracks were found to be initiated by structural resonance caused by flow fluctuations from the SSME low pressure fuel turbopump interacting with the flowliner. The pump induced backward traveling wakes that excited the liner and duct acoustics which also caused the liner to vibrate in complex mode shapes. The investigation involved an extensive effort by a team of engineers from the NASA civil servant and contractor workforce with the goal to characterize the root cause of the cracking behavior of the fuel side gimbal joint flowliners. In addition to working to identify the root cause, a parallel path was taken to characterize the material properties and fatigue capabilities of the liner material such that the life of the liners could be ascertained. As the characterization of the material and the most probable cause matured, the combination of the two with pump speed restrictions provided a means to return the Shuttle to flight in a safe manner. This paper traces the flowliner investigation results with respect to the structural dynamics analysis, component level testing and hot-fire flow testing on a static testbed. The paper will address the unique aspects of a very complex problem involving backflow from a high performance pump that has never been characterized nor understood to such detail. In addition, the paper will briefly address the flow phenomena that excited the liners, the unique structural dynamic modal characteristics and the variability of SSME operation which has ultimately ensured the safe and reliable operation of the shuttle main engines for each flight.

Frady, Gregory P.↗

DOE OSTI2021

Assembly of the Populus Microbiome Is Temporally Dynamic and Determined by Selective and Stochastic Factors

Recent work shows that the plant microbiome, particularly the initial assembly of this microbiome, influences plant health, survival, and fitness. Here, we characterize the initial assembly of the Populus microbiome across ten genotypes belonging to two poplar species in a common garden using 16S rRNA gene and ITS2 region amplicon sequencing of the leaf endosphere, leaf surface, root endosphere, and rhizosphere. We sampled these microbiomes three times throughout the first growing season and found that the composition of the microbiome changed dramatically over time across all plant-associated habitats and host genotypes. For archaea and bacteria, these changes were dominated by strong homogenizing selection (accounting for 29 to 62% of pairwise comparisons). However, fungal assembly was generally characterized by multiple ecological assembly processes (i.e., a mix of weak selective and dispersal processes). Interestingly, genotype, while a significant moderator of microbiome composition, generally explained less variation than sample date across plant-associated habitats. We defined a set of core genera that accounted for, on average, 36% of the microbiome. The relative abundance of this core community was consistent over time. Additionally, using source tracking modeling, we determined that new microbial taxa colonize from both aboveground and belowground sources, and combined with our ecological assembly null models, we found that both selective and dispersal processes explained the differences between exo- (i.e., leaf surface and rhizosphere) and endospheric microbiomes. Taken together, our results suggest that the initial assembly of the Populus microbiome is time-, genotype-, and habitat-dependent and is moderated by both selective and stochastic factors.

16S rRNA↗

DOE OSTI2022

Transcription Factors Controlling the Rhizobium–Legume Symbiosis: Integrating Infection, Organogenesis and the Abiotic Environment

Abstract Legume roots engage in a symbiotic relationship with rhizobia, leading to the development of nitrogen-fixing nodules. Nodule development is a sophisticated process and is under the tight regulation of the plant. The symbiosis initiates with a signal exchange between the two partners, followed by the development of a new organ colonized by rhizobia. Over two decades of study have shed light on the transcriptional regulation of rhizobium–legume symbiosis. A large number of transcription factors (TFs) have been implicated in one or more stages of this symbiosis. Legumes must monitor nodule development amidst a dynamic physical environment. Some environmental factors are conducive to nodulation, whereas others are stressful. The modulation of rhizobium–legume symbiosis by the abiotic environment adds another layer of complexity and is also transcriptionally regulated. Several symbiotic TFs act as integrators between symbiosis and the response to the abiotic environment. In this review, we trace the role of various TFs involved in rhizobium–legume symbiosis along its developmental route and highlight the ones that also act as communicators between this symbiosis and the response to the abiotic environment. Finally, we discuss contemporary approaches to study TF-target interactions in plants and probe their potential utility in the field of rhizobium–legume symbiosis.

Cell Biology↗

DOE OSTI2026

Nutrient limitation shapes functional traits of mycorrhizal fungi and phosphorus-cycling bacteria across an elevation gradient

In nutrient-limited high-elevation ecosystems, plants rely on arbuscular mycorrhizal (AM) fungi to provide mineral phosphorus (P) in the form of phosphate (PO43-). AM fungi gather these nutrients from phosphorus-cycling bacteria (PCBs) that can mineralize PO43- from organic matter and solubilize mineral-bound P. How climate, soil factors, and nutrient limitation influence AM fungi and PCB assembly remains unclear. We collected soil from montane meadows across a 1,000-m elevation gradient on three replicate mountainsides and analyzed AM fungal marker genes, P-cycling genes from shotgun metagenomes, and edaphic measurements. High-elevation soils had nearly 50-fold less soil PO₄³⁻ and 60% more AM fungal hyphae than low-elevation soils. AM fungal turnover was linked to changes in pH, organic carbon, and PO₄³-. The composition of 198 P-cycling genes was influenced by the AM fungal community structure. Drivers of individual PCB functional genes, including pH and organic carbon, varied with gene phylogeny. We found a trade-off in P-cycling strategies across elevation: P-rich, low-elevation soils supported root-colonizing AM fungi and organic P-mineralizing bacteria. P-poor, high-elevation soils were dominated by stress-tolerant AM fungi and mineral P-solubilizing bacteria. Our results suggest that AM fungi and PCB community turnover across elevation are both shaped by pH, organic carbon, and P availability. With continued climate warming, the structure and function of mountaintop ecosystems might shift to resemble lower elevations, disrupting long-established and specialized microbial assemblages, with consequences for P-cycling dynamics and the total P available to plant communities.IMPORTANCEPhosphorus (P) limits plant productivity in high-elevation ecosystems, yet the microbial networks that mobilize P, including arbuscular mycorrhizal (AM) fungi and phosphorus-cycling bacteria (PCBs), remain under-characterized in these nutrient-poor soils. We show that across a 10,00-m elevation gradient, AM fungi and P-cycling gene assemblages shift predictably with pH, organic carbon, and phosphate availability. Higher elevations, with less available P, select for stress-tolerant AM fungal taxa and PCB strategies geared toward mineral solubilization, while low-elevation sites favor root colonization by AM fungi and organic P mineralization. These results suggest that nutrient limitation can constrain microbial community assembly in consistent ways across landscapes. High mountain soils are low in P and rely on a network of underground AM fungi and PCB to deliver nutrients to plants. This study shows how those underground relationships reorganize with elevation and how climate change could collapse long-standing microbial strategies by pushing high-elevation ecosystems toward lowland conditions. As soils warm and dry, the microbial scaffolding that supports alpine plant life may become increasingly unstable.

arbuscular mycorrhizal fungi↗

DOE OSTI2025

Dynamic modelling and control strategy of a temperature-driven metal hydride cooling system for buildings

A temperature-driven coupled metal hydride (MH) based thermal energy storage (TES) system can allow to shave and shift the peak energy demand in buildings. The high energy density and long-term (seasonal) energy storage capability are its major advantages over other energy storage methods. The dynamic nature of the MH operation, however, requires controlled hydrogen transfer between the coupled MHs at a rate needed to meet the building's transient load. While temperature-driven MH systems are studied in the literature, their application in buildings and control are scarcely reported. Here, this paper presents a control-based dynamic modeling of the temperature-driven coupled MH-TES system for building cooling applications. The dynamic model is developed in MATLAB(R) Simulink environment, considering the thermodynamic and kinetic behaviors of the MH systems. Based on a preliminary analysis of a property database of over 337 hydrides, we select around 1600 MH pairs suitable for building cooling applications. Each of these MH pairs is studied for their performance using the dynamic model, and among all, Zr 0.76 Ti 0.24 Ni 1.16 Mn 0.63 V 0.14 Fe 0.18 -Ti 0.85 Zr 0.15 Cr 1.2 Mn 0.8 MH pair showed fast dynamics along with high coefficient of performance (COP) of 0.71. A parametric investigation is performed on this MH pair to understand the effect of operating temperatures. Finally, three proportional-integral (PI) feedback controllers are investigated to regulate the temperature, pressure and mass exchange between the coupled MH pairs. The developed PI controller is sufficiently capable of rejecting the signal noise from the hydrogen flow and internal heat exchange processes with root mean square error of 5.78 W between reference and actual cooling load.

08 HYDROGEN↗

NASA NTRS2024

Evaluation of Machine Learning and Deep Learning Algorithms for Fire Prediction in Southeast Asia

Vegetation fires are prevalent in South/Southeast Asian countries, making fire prediction crucial due to their potential environmental, economic, and social impacts. Accurate predictions of fires facilitate timely interventions, helping to mitigate uncontrolled fires that can lead to biodiversity loss and air quality issues. In this study, we utilize VIIRS satellite-derived fire data alongside six machine learning and deep learning models—Simple Persistence, Multi-Layer Perceptron (MLP), Convolutional Neural Network (CNN), Long Short-Term Memory (LSTM), CNN-LSTM, and ConvLSTM—to determine the most effective fire prediction model, using Root Mean Square Error (RMSE) as the metric. Our results indicate that the CNN model is the most reliable in regions with spatial dependencies, such as Brunei, Indonesia, Malaysia, the Philippines, Timor-Leste, and Thailand. Conversely, the ConvLSTM model excels in countries with complex spatiotemporal dynamics like Laos, Myanmar, and Vietnam. The CNN-LSTM hybrid model also performed well in Cambodia, suggesting a need for a balanced approach in areas requiring both spatial and temporal feature extraction. Furthermore, simpler models like Persistence and MLP showed limitations in capturing dynamic patterns and temporal dependencies. Our findings highlight the importance of evaluating models before implementing any decision support systems (DSS) in fire management. By tailoring models to specific regional fire data, we can enhance prediction accuracy and responsiveness, ultimately improving fire risk management in Southeast Asia and beyond.

Deep learning↗

DOE OSTI2024

Seasonality and longer-term development generate temporal dynamics in the Populus microbiome

ABSTRACT Temporal variation in community composition is central to our understanding of the assembly and functioning of microbial communities, yet the controls over temporal dynamics for microbiomes of long-lived plants, such as trees, remain unclear. Temporal variation in tree microbiomes could arise primarily from seasonal (i.e., intra-annual) fluctuations in community composition or from longer-term changes across years as host plants age. To test these alternatives, we experimentally isolated temporal variation in plant microbiome composition using a common garden and clonally propagated plants, and we used amplicon sequencing to characterize bacterial/archaeal and fungal communities in the leaf endosphere, root endosphere, and rhizosphere of two Populus spp. over four seasons across two consecutive years. Microbial community composition differed among seasons and years (which accounted for up to 21% of the variation in microbial community composition) and was correlated with seasonal dissimilarity in climatic conditions. However, microbial community dissimilarity was also positively correlated with time, reflecting longer-term compositional shifts as host trees aged. Together, our findings demonstrate that temporal patterns in tree microbiomes arise from both seasonal fluctuations and longer-term changes, which interact to generate unique seasonal patterns each year. In addition to shedding light on two important controls over the assembly of plant microbiomes, our results also suggest future studies of tree microbiomes should account for background temporal dynamics when testing the drivers of spatial patterns in microbial community composition and temporal responses of plant microbiomes to environmental change. IMPORTANCE Microbiomes are integral to the health of host plants, but we have a limited understanding of the factors that control how the composition of plant microbiomes changes over time. Especially little is known about the microbiome of long-lived trees, relative to annual and non-woody plants. We tested how tree microbiomes changed between seasons and years in poplar (genus Populus ), which are widespread and ecologically important tree species that also serve as important biofuel feedstocks. We found the composition of bacterial, archaeal, and fungal communities differed among seasons, but these seasonal differences depended on year. This dependence was driven by longer-term changes in microbial composition as host trees developed across consecutive years. Our findings suggest that temporal variation in tree microbiomes is driven by both seasonal fluctuations and longer-term (i.e., multiyear) development.

59 BASIC BIOLOGICAL SCIENCES↗