Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “independent evaluation”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 613 records · Page 34
All sourcesNASA NTRSDOE OSTI
NASA NTRS1994

Grain destruction in shocks in the interstellar medium

Destruction of interstellar dust occurs predominantly in supernova shock waves in the warm neutral/ionized medium (density approximately = 0.25/cu cm, temperature approximately = 10(exp 4) K). Recent theoretical developments and laboratory data for sputtering processes and grain-grain collisional vaporization allows us to better evaluate the grain destruction rate in interstellar shocks in the warm medium. We find that, independent of composition, grain denstruction in supernova blast waves is dominated by nonthermal sputtering for shock velocities greater than 50 km/s and less than or equal to 150 km/s and thermal sputtering at higher shock velocities. We use a detailed scheme for the vaporization of grains colliding at high velocities (v(sub s) greater than or equal to 20 km/s) and show that the grain-grain collision destruction process is only dominant for shock velocities of less than or equal to 50-80 km/s and is less important than previously assumed. Nevertheless, the grain-grain destruction rates are of order 30%-90% of the sputtering rates at v(sub s) greater than 100 km/s and less than 200 km/s and are important in vaporizing the cores of grains. Detailed results for grain destruction as a function of grain size and composition are presented. We also present results for silicon carbide, iron, ice, and porous test particles. For carbonaceous grains we find that the fractional destruction is less than or equal to 0.29, and for silicate it is less than or equal to 0.45, for v(sub s) less than or equal to 200 km/s. We have calculated grain lifetimes, using the three-phase model of the interstellar medium, and find lifetimes of 4 x 10(exp 8) yr for carbonaceous grains and 2.2 x 10(exp 8) yr for silicate grains. Given that the typical stardust injection timescale of 2.5 x 10(exp 9) yr, we conclude that efficient mechanisms for grain growth in the interstellar medium must exist in order that a significant fraction of the refractory elements be incorporated in dust, as observed. Therefore, although our improved model has less vaporization of dust due to grain-grain collisions, sputtering still destroys dust efficiently and grain mantle growth in the interstellar medium is required, a conclusion reached in previous models of grain destruction in the interstellar medium. Carbonaceous mantles on silicate grains can protect the silicate cores from sputtering destruction in interstellar shock waves, provided that the protective mantles can efficiently reform in the interstellar medium. Also, if the grains are porous the postshock grain velocities are lower than for solid particles, and grain destruction is reduced. Porosity and mantling may increase the grain lifetime by factors of approximately 3 and approximately 3-4, respectively. The fraction of interstellar silicon in silicate stardust is therefore less than or equal to 0.25, but more silicon might be depleted in the form of a grain mantle.

Jones, A. P.↗
NASA NTRS1994

Reliability-Based Design of a Safety-Critical Automation System: A Case Study

In 1986, NASA funded a project to modernize the NASA Ames Research Center Unitary Plan Wind Tunnels, including the replacement of obsolescent controls with a modern, automated distributed control system (DCS). The project effort on this system included an independent safety analysis (ISA) of the automation system. The purpose of the ISA was to evaluate the completeness of the hazard analyses which had already been performed on the Modernization Project. The ISA approach followed a tailoring of the risk assessment approach widely used on existing nuclear power plants. The tailoring of the nuclear industry oriented risk assessment approach to the automation system and its role in reliability-based design of the automation system is the subject of this paper.

Carroll, Carol W.↗
NASA NTRS2002

Status of the RBCC Direct-Connect Mixer Combustor Experiment

The NASA Glenn Research Center is developing hydrogen based combined cycle propulsion technology for a single-stage-to-orbit launch vehicle application under a project called GTX. Rocket Based Combined Cycle (RBCC) propulsion systems incorporate one or more rocket engines into an airbreathing flow path to increase specific impulse as compared to an all rocket-powered vehicle. In support of this effort, an RBCC direct-connect test capability was established at the Engine Components Research Laboratory to investigate low speed, ejector ramjet, and initial ramjet operations and performance. The facility and test article enables the evaluation of two candidate low speed operating schemes; the simultaneous mixing and combustion (SMC) and independent ramjet stream (IRS). The SMC operating scheme is based on the fuel rich operations of the rocket where performance depends upon mixing between the rocket plume and airstream. In contrast, the IRS scheme fuels the airstream separately and uses the rocket plume to ignite the fuel-air mixture. This paper describes the test hardware and facility upgrades installed to support the RBCC tests. It also defines and discusses low speed technical challenges being addressed by the experiments. Finally, preliminary test results, including rocket risk mitigating tests, unfueled airflow tests, and the integrated system hot fire test will be presented.

Walker, James F.↗
NASA NTRS2002

Status of the RBCC Direct-Connect Mixer Combustor Experiment

The NASA Glenn Research Center is developing hydrogen based combined cycle propulsion technology for a single-stage-to-orbit launch vehicle application under a project called GTX. Rocket Based Combined Cycle (RBCC) propulsion systems incorporate one or more rocket engines into an airbreathing flow path to increase specific impulse as compared to an all rocket-powered vehicle. In support of this effort, an RBCC direct-connect test capability was established at the Engine Components Research Laboratory to investigate low speed, ejector ramjet, and initial ramjet operations and performance. The facility and test article enables the evaluation of two candidate low speed operating schemes; the simultaneous mixing and combustion (SMC) and independent ramjet stream (IRS). The SMC operating scheme is based on the fuel rich operations of the rocket where performance depends upon mixing between the rocket plume and airstream. In contrast, the IRS scheme fuels the airstream separately and uses the rocket plume to ignite the fuel-air mixture. This paper describes the test hardware and facility upgrades installed to support the RBCC tests. It also defines and discusses low speed technical challenges being addressed by the experiments. Finally, preliminary test results, including rocket risk mitigating tests, unfueled airflow tests, and the integrated system hot fire test will be presented.

Walker, James F.↗
NASA NTRS2012

An Assessment of Civil Tiltrotor Concept of Operations in the Next Generation Air Transportation System

Based on a previous Civil Tiltrotor (CTR) National Airspace System (NAS) performance analysis study, CTR operations were evaluated over selected routes and terminal airspace configurations assuming noninterference operations (NIO) and runway-independent operations (RIO). This assessment aims to further identify issues associated with these concepts of operations (ConOps), and their dependency on the airspace configuration and interaction with conventional fixed-wing traffic. Safety analysis following a traditional Safety Management System (SMS) methodology was applied to CTR-unique departure and arrival failures in the selected airspace to identify any operational and certification issues. Additional CTR operational cases were then developed to get a broader understanding of issues and gaps that will need to be addressed in future CTR operational studies. Finally, needed enhancements to National Airspace System performance analysis tools were reviewed, and recommendations were made on improvements in these tools that are likely to be required to support future progress toward CTR fleet operations in the Next Generation Air Transportation System (NextGen).

Chung, William W.↗
NASA NTRS2020

Impact of Gauge-Based Precipitation Corrections on the Skill of SMAP Level-4 Soil Moisture Estimates

The NASA Soil Moisture Active Passive (SMAP) mission provides observations of L-band (1.4 GHz) passive microwave brightness temperature (Tb) observations at a resolution of ~40 km globally every 2-3 days. These observations are routinely assimilated into the NASA Catchment land surface model to generate the Level-4 Soil Moisture (L4_SM) product, which provides global estimates of surface and root-zone soil moisture, soil temperature, and surface fluxes (among others) at 9-km, 3-hourly resolution with ~2.5-day latency. The Catchment land surface model in the L4_SM algorithm is driven with 0.25°, hourly surface meteorological forcing data from the NASA Goddard Earth Observing System (GEOS) "forward-processing" product. Outside of Africa and the high latitudes, the GEOS precipitation forcing is corrected using the Climate Prediction Center Unified (CPCU) gauge-based, 0.5°, daily precipitation product.Soil moisture estimates from the L4_SM product were previously shown to improve over land model-only estimates that do not benefit from the assimilation of Tb observations, thereby demonstrating the value of assimilating SMAP observations for soil moisture estimation. In this presentation, we further isolate the contribution of the gauge-based precipitation corrections to the skill of the L4_SM soil moisture estimates. Specifically, we compare the skill of the L4_SM soil moisture to that of separate model-only and assimilation estimates obtained without the benefit of the gauge-based precipitation corrections.Preliminary results suggest that the soil moisture skill added by the CPCU-based precipitation corrections primarily depends on the quality of the CPCU precipitation product and is greatest in regions where the CPCU gauge network is dense and reliable. Conversely, in regions where the CPCU product is known to be of poor quality, for example in central Australia, the assimilation of SMAP Tb observations provides the most benefit. The presentation will provide an in-depth evaluation of the soil moisture skill of the model-only and assimilation estimates vs. independent in situ and satellite measurements.

Reichle, Rolf↗
NASA NTRS2019

The Value of Long-Term (40 years) Airborne Gamma Radiation SWE Record for Evaluating Three Observation-Based Gridded SWE Data Sets by Seasonal Snow and Land Cover Classifications

Observation‐based long‐term gridded snow water equivalent (SWE) products are important assets for hydrological and climate research. However, an evaluation of the currently available SWE products has been limited due to the lack of independent SWE data that extend over a large range of environmental conditions. In this study, three daily long‐term SWE products (Special Sensor Microwave Imager and Sounder [SSMI/S] SWE, GlobSnow‐2 SWE, and University of Arizona [UA] SWE) we reevaluated by seasonal snow cover and land cover classifications over the conterminous United States from1982 to 2017, using the historical airborne gamma radiation SWE observations (20,738 measurements).We found that there are similar patterns in SSMI/S and GlobSnow‐2 SWE when compared against the gamma SWE. However, GlobSnow‐2 SWE had better agreement with gamma SWE than SSMI/S SWE in some forested‐type classes and maritime and prairie snow classes. As compared to SSMI/S and GlobSnow‐2SWE, UA SWE has much better agreement with gamma SWE in all land cover types and snow classes. Tree cover and topographic heterogeneity affect the agreement between the gamma and gridded SWE and accuracy of gamma SWE itself with the largest differences typically occurring when the percent tree cover was 80% or higher, the terrain slope was steeper than 2.5°, and the elevation range exceeded 100 m. The results demonstrate the reliability of the UA SWE products and the benefits of the gamma radiation approach to measure SWE, especially in forested regions.

Eunsang Cho↗
NASA NTRS2024

Noise Reduction for Solar-Induced Fluorescence Retrievals Using Machine Learning and Principal Component Analysis: Simulations and Applications to GOME-2 Satellite Retrievals

We use a spectral-based approach that employs principal component analysis along with a relatively shallow artificial neural network (NN) to substantially reduce noise and other artifacts in terrestrial chlorophyll solar-induced fluorescence (SIF) retrievals. SIF is a very small emission at red and far-red wavelengths that is difficult to measure and is highly sensitive to random errors and systematic artifacts. Our approach relies upon an assumption that a trained NN can effectively reconstruct the total SIF signal from a relatively small number of leading principal components of the satellite-observed far-red radiance spectra without using information from the trailing modes that contain most of the random errors. We test the approach with simulated reflectance spectra produced with a full atmospheric and surface radiative transfer model using different observing and geophysical parameters and various noise levels. Resulting noisy and noise-reduced retrieved SIF values are compared with true values to assess performance. We then apply our noise reduction approach to SIF derived from two different satellite spectrometers. For evaluation, since the truth in this case is unknown, we compare SIF retrievals from two independent sensors with each other. We also compare the noise-reduced SIF temporal variations with those from an independent gross primary product (GPP) product that should display similar variations. Results show that our noise reduction approach improves capture of SIF seasonal and inter-annual variability. Our approach should be applicable to many noisy data products derived from spectral measurements. Our methodology does not replace the original retrieval algorithms; rather the original noisy retrievals are needed as the target for the NN training process.

noise reduction, solar-induced fluorescence, SIF, ↗
DOE OSTI2023

Tabulated Database of Closed-Loop Geothermal Systems Performance for Cloud-Based Technical and Economic Modeling of Heat Production and Electricity Generation: Preprint

To better understand the heat production, electricity generation performance and economic viability of closed loop geothermal systems in hot-dry-rock, the Closed Loop Geothermal Group, a consortium of several national labs and academic institutions has tabulated time-dependent numerical solutions and levelized cost results of two popular closed loop heat exchanger designs (u-tube and co-axial). The heat exchanger designs were evaluated for two working fluids (water and super-critical CO2) while varying seven continuous independent parameters of interest (i.e., mass flow rate, vertical depth, horizontal extent, borehole diameter, formation gradient, formation conductivity, and injection temperature). The corresponding numerical solutions (approximately 1.2 million per heat exchanger design) are stored as multi-dimensional HDF5 datasets and can be queried at off-grid points using multi-dimensional linear interpolation. A Python script was developed to query this database and estimate time-dependent electricity generation using an Organic Rankine cycle (for water) or direct turbine expansion cycle (for CO2) and perform a cost assessment. This document aims to give an overview of the HDF5 database file and highlights how to read, visualize, and query quantities of interest (e.g., levelized cost of electricity, levelized cost of heat) using the accompanying python scripts. Details regarding the capital, operation, and maintenance and levelized cost calculation using the TEA (techno-economic analysis) script are provided.

co-axial↗
DOE OSTI2021

Development of observation-based global multilayer soil moisture products for 1970 to 2016

Abstract. Soil moisture (SM) datasets are critical to understanding the global water, energy, and biogeochemical cycles and benefit extensive societal applications. However, individual sources of SM data (e.g., in situ and satellite observations, reanalysis, offline land surface model simulations, Earth system model – ESM – simulations) have source-specific limitations and biases related to the spatiotemporal continuity, resolutions, and modeling and retrieval assumptions. Here, we developed seven global, gap-free, long-term (1970–2016), multilayer (0–10, 10–30, 30–50, and 50–100 cm) SM products at monthly 0.5∘ resolution (available at https://doi.org/10.6084/m9.figshare.13661312.v1; Wang and Mao, 2021) by synthesizing a wide range of SM datasets using three statistical methods (unweighted averaging, optimal linear combination, and emergent constraint). The merged products outperformed their source datasets when evaluated with in situ observations (mean bias from −0.044 to 0.033 m3 m−3, root mean square errors from 0.076 to 0.104 m3 m−3, Pearson correlations from 0.35 to 0.67) and multiple gridded datasets that did not enter merging because of insufficient spatial, temporal, or soil layer coverage. Three of the new SM products, which were produced by applying any of the three merging methods to the source datasets excluding the ESMs, had lower bias and root mean square errors and higher correlations than the ESM-dependent merged products. The ESM-independent products also showed a better ability to capture historical large-scale drought events than the ESM-dependent products. The merged products generally showed reasonable temporal homogeneity and physically plausible global sensitivities to observed meteorological factors, except that the ESM-dependent products underestimated the low-frequency temporal variability in SM and overestimated the high-frequency variability for the 50–100 cm depth. Based on these evaluation results, the three ESM-independent products were finally recommended for future applications because of their better performances than the ESM-dependent ones. Despite uncertainties in the raw SM datasets and fusion methods, these hybrid products create added value over existing SM datasets because of the performance improvement and harmonized spatial, temporal, and vertical coverages, and they provide a new foundation for scientific investigation and resource management.

54 ENVIRONMENTAL SCIENCES↗
NASA NTRS1974

Evaluation of exercise-respiratory system modifications and preliminary respiratory-circulatory system integration scheme

The respiratory control system, functioning as an independent system, is presented with modifications of the exercise subroutine. These modifications illustrate an improved control of ventilation rates and arterial and compartmental gas tensions. A very elementary approach to describing the interactions of the respiratory and circulatory system is presented.

Gallagher, R. R.↗
DOE OSTI2022

Mobility Energy Productivity Evaluation of Prediction-Based Vehicle Powertrain Control Combined with Optimal Traffic Management

Transportation vehicle and network system efficiency can be defined in two ways: 1) reduction of travel times across all the vehicles in the system, and 2) reduction in total energy consumed by all the vehicles in the system. The mechanisms to realize these efficiencies are treated as independent (i.e., vehicle and network domains) and, when combined, they have not been adequately studied to date. This research aims to integrate previously developed and published research on Predictive Optimal Energy Management Strategies (POEMS) and Intelligent Traffic Systems (ITS), to address the need for quantifying improvement in system efficiency resulting from simultaneous vehicle and network optimization. POEMS and ITS are partially independent methods which do not require each other to function but whose individual effectiveness may be affected by the presence of the other. In order to evaluate the system level efficiency improvements, the Mobility Energy Productivity (MEP) metric is used. MEP specifically measures the connectedness of a system while accounting for time and energy externalities of modes that provide mobility in a given location. A SUMO model is developed to reflect real traffic patterns in Fort Collins, Colorado and data is collected by a probe SUMO vehicle which is validated against data collected on a real vehicle driving the same routes through the city. Individual vehicle and system level efficiencies are calculated using SUMO outputs for scenarios which integrate POEMS and ITS independently as well as jointly. Results from application of POEMS and ITS show improvement in energy consumption and travel times respectively when compared to the respective baseline scenarios. Our conclusion is that there are promising synergistic benefits to travel time and energy efficiency when POEMS and ITS are combined.

ADVANCED PROPULSION SYSTEMS↗
NASA NTRS2005

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-down Post Stud Hang-ups

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has "hung-up." That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down post studs experiencing a "hang-up." The results af loads analyses performed for four (4) stud-hang ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

Cross, Robert↗
NASA NTRS2005

Common Cause Case Study: An Estimated Probability of Four Solid Rocket Booster Hold-Down Post Stud Hang-ups

Until Solid Rocket Motor ignition, the Space Shuttle is mated to the Mobil Launch Platform in part via eight (8) Solid Rocket Booster (SRB) hold-down bolts. The bolts are fractured using redundant pyrotechnics, and are designed to drop through a hold-down post on the Mobile Launch Platform before the Space Shuttle begins movement. The Space Shuttle program has experienced numerous failures where a bolt has hung up. That is, it did not clear the hold-down post before liftoff and was caught by the SRBs. This places an additional structural load on the vehicle that was not included in the original certification requirements. The Space Shuttle is currently being certified to withstand the loads induced by up to three (3) of eight (8) SRB hold-down experiencing a "hang-up". The results of loads analyses performed for (4) stud hang-ups indicate that the internal vehicle loads exceed current structural certification limits at several locations. To determine the risk to the vehicle from four (4) stud hang-ups, the likelihood of the scenario occurring must first be evaluated. Prior to the analysis discussed in this paper, the likelihood of occurrence had been estimated assuming that the stud hang-ups were completely independent events. That is, it was assumed that no common causes or factors existed between the individual stud hang-up events. A review of the data associated with the hang-up events, showed that a common factor (timing skew) was present. This paper summarizes a revised likelihood evaluation performed for the four (4) stud hang-ups case considering that there are common factors associated with the stud hang-ups. The results show that explicitly (i.e. not using standard common cause methodologies such as beta factor or Multiple Greek Letter modeling) taking into account the common factor of timing skew results in an increase in the estimated likelihood of four (4) stud hang-ups of an order of magnitude over the independent failure case.

Cross, Robert↗
NASA NTRS2004

Air Mass Factor Formulation for Spectroscopic Measurements from Satellites: Application to Formaldehyde Retrievals from the Global Ozone Monitoring Experiment

We present a new formulation for the air mass factor (AMF) to convert slant column measurements of optically thin atmospheric species from space into total vertical columns. Because of atmospheric scattering, the AMF depends on the vertical distribution of the species. We formulate the AMF as the integral of the relative vertical distribution (shape factor) of the species over the depth of the atmosphere, weighted by altitude-dependent coefficients (scattering weights) computed independently from a radiative transfer model. The scattering weights are readily tabulated, and one can then obtain the AMF for any observation scene by using shape factors from a three dimensional (3-D) atmospheric chemistry model for the period of observation. This approach subsequently allows objective evaluation of the 3-D model with the observed vertical columns, since the shape factor and the vertical column in the model represent two independent pieces of information. We demonstrate the AMF method by using slant column measurements of formaldehyde at 346 nm from the Global Ozone Monitoring Experiment satellite instrument over North America during July 1996. Shape factors are cumputed with the Global Earth Observing System CHEMistry (GEOS-CHEM) global 3-D model and are checked for consistency with the few available aircraft measurements. Scattering weights increase by an order of magnitude from the surface to the upper troposphere. The AMFs are typically 20-40% less over continents than over the oceans and are approximately half the values calculated in the absence of scattering. Model-induced errors in the AMF are estimated to be approximately 10%. The GEOS-CHEM model captures 50% and 60% of the variances in the observed slant and vertical columns, respectively. Comparison of the simulated and observed vertical columns allows assessment of model bias.

Palmer, Paul I.↗
DOE OSTI2024

Nuclear Power Plant Infrastructure Evaluations for Removal of Spent Nuclear Fuel

This report provides evaluations of the NPP site infrastructure and near-site transportation infrastructure for removing SNF from 19 NPP sites and the Morris Independent Spent Fuel Storage Installation (ISFSI). The material to be removed from the NPP sites includes both the SNF and the greater-than-Class C low-level radioactive waste (GTCC waste)3 that is stored, or will be stored, at the sites. This report is an update of the report Nuclear Power Plant Infrastructure Evaluations for Removal of Spent Nuclear Fuel (Maheras et al. 2021) and includes expansion of the site evaluations to include operating nuclear power plant (NPP) sites and to incorporate updated site inventory data. Figures that include the number of spent nuclear fuel (SNF) assemblies and metric tons heavy metal (MTHM) in a single figure have also been added to the report.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗
DOE OSTI2021

Simple, efficient and open-source CRISPR/Cas9 strategy for multi-site genome editing in Populus tremula × alba

Although the CRISPR/Cas9 system has been successfully used for crop breeding, its application remains limited in forest trees. Here, we describe an efficient gene editing strategy for hybrid poplar, (Populus tremula × alba INRA clone 717-1B4) based on the Golden Gate MoClo cloning. To test the system efficiency for generating single gene mutants, two single guide RNAs (sgRNAs) were designed and incorporated into the MoClo Tool Kit level 2 binary vector with the Cas9 expression cassette to mutate the SHORT ROOT (SHR) gene. Moreover, we also tested its efficiency for introducing mutations in two genes simultaneously by expressing one sgRNA targeting a single site of the YUC4 gene and the other sgRNA targeting the PLT1 gene. For a robust evaluation of the approach, we repeated the strategy to target the LBD12 and LBD4 genes simultaneously, using an independent construct. We generated hairy roots by Agrobacterium rhizogenes-mediated leaf transformation. Sequencing results confirmed the CRISPR/Cas9-mediated mutation in the targeted sites of PtaSHR. Biallelic and homozygous knockout mutations were detected. A deletion spanning both target sites and small insertions/deletions were the most common mutations. Out of the 22 SHR alleles sequenced, 21 were mutated. The phenotype’s characterization showed that transgenic roots with biallelic mutations for the SHR gene lacked a defined endodermal single cell layer, suggesting a conserved gene function similar to its homolog in Arabidopsis Arabidopsis thaliana (L.) Heynh. Sequencing results also revealed the high efficiency of the system for generating double mutants. Biallelic mutations for both genes in the yuc4/plt1 and lbd12/lbd4 roots were detected in three (yuc4/plt1) and two (lbd12/lbd4) out of four transgenic roots evaluated. A small deletion or a single nucleotide insertion at the single target site was the most common mutations. This CRISPR/Cas9 strategy arises as a rapid, simple and standardized gene-editing tool to evaluate the gene role in essential developmental programs such as radial cell differentiation of poplar roots.

59 BASIC BIOLOGICAL SCIENCES↗