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At least 613 records · Page 34

Measurement Background and the Sediment Age-Dating Reach of 32Si

Silicon-32 has been identified as an isotope that fills the sediment geochronology gap created between Pb-210 at <150 years and C-14 at >1000 years. However, detector sensitivity and chemical isolation challenges of radiometric Si-32 for age dating have resulted in a fairly limited number of applications of this technique to real-world sediment studies. The significance of targeting the intermediate age bracket is to provide accurate geochronological reconstructions of paleoindicators that identify human and large-scale, climate-induced shifts in coastal areas. Therefore, we targeted Puget Sound, Washington, United States, to demonstrate the application of Si-32 age dating to determine the relative importance of climate cycles versus human activities in an in-land sea. The enhanced reconstructions will be achieved through the design of new detectors, chemical separation of biogenic silica from kilogram-scale sediment samples, and demonstration of the viable age-dating reach of Si-32. Implications of detector background on the duration of required measurements and the age-dating reach are considered. The design of a low-background, gas-proportional beta counter for measurement of the daughter isotope P-32 is discussed; detector background goals for these new detectors are based on the sensitivity study.

Keillor, Martin E.↗

When a cuvette is not a canopy: a caution about measuring leaf temperature during gas exchange measurements

Plant gas exchange systems are widely used to study leaf physiological processes and properties such as stomatal function and the maximum carboxylation rate of Rubisco. Increasingly, these systems are used to assess how leaf gas exchange varies with temperature in order to better understand how rising temperature will impact plant function. Examples of such studies include variation in optimal temperatures of photosynthesis as a function of species and growth environment, and respiratory acclimation to higher temperatures. Leaf thermoregulation leading to homeothermy has been reported based on leaf gas exchange measurements spanning a large (~25 °C) temperature range. However, as we show here, the design of a popular gas exchange system used for temperature-response measurements can lead to biased measurements of leaf temperature. Furthermore, we demonstrate this with an example showing that apparent leaf thermoregulatory behavior can arise even in empty cuvettes. More broadly, our results have implications for other temperature manipulations in similar gas exchange systems.

59 BASIC BIOLOGICAL SCIENCES↗

New Method for Measuring the Anchoring Energy of Strongly-Bound Membrane-Associated Proteins [Method for measuring the anchoring energy of strongly-bound membrane-associated proteins].

Here, we describe a new method to measure the activation energy required to remove a strongly-bound membrane-associated protein from a lipid membrane (anchoring energy). It is based on measuring the rate of release of a liposome-bound protein during centrifugation on a sucrose gradient as a function of time and temperature. The method was used to determine anchoring energy for the soluble dengue virus envelope protein (sE) strongly bound to 80:20 POPC:POPG liposomes at pH 5.5. We also measured the binding energy of sE at the same pH for the initial, predominantly reversible, phase of binding to a 70:30 PC:PG lipid bilayer. The anchoring energy (37 +/- 1.7 kcal/mol, 20% PG) was found to be much larger than the binding energy (7.8 +/- 0.3 kcal/mol for 30% PG, or est. 7.0 kcal/mol for 20% PG). This is consistent with data showing that free sE is a monomer at pH 5.5, but assembles into trimers after associating with membranes. But, trimerization alone is insufficient to account for the observed difference in energies, and we conclude that some energy dissipation occurs during the release process. This new method to determine anchoring energy should be useful to understand the complex interactions of integral monotopic proteins and strongly-bound peripheral membrane proteins with lipid membranes.

59 BASIC BIOLOGICAL SCIENCES↗

A reporting format for field measurements of soil respiration

Field observations of the soil-to-atmosphere CO2 flux–soil respiration, RS–are a prime example of ‘long tail’ data that historically have had neither centralized databases nor an agreed-upon reporting format. This has hindered scientific transparency, analytical reproducibility, and novel syntheses with respect to this globally-important component of the carbon cycle. Here we propose a new data and metadata reporting format for RS data, based on engagement with a wide range of researchers in the field as well as expert advisory panels. Our goal was a reporting format that would be relevant and useful for synthesis activities, and optimizing data discoverability and usability while not placing an undue burden on data contributors. We describe previous RS data collection efforts, lessons learned from related databases and data-oriented networks (e.g. FLUXNET) in earth and ecological sciences, and the process of community consultation. The proposed reporting format focuses on chamber-level data and metadata, specifying measurement conditions and, for a given measurement period defined by beginning and ending timestamps, a mean RS flux (or CO2 concentration) and associated ancillary measurements. Fundamentally, this format aims to enable findable, accessible, interoperable, and reusable data, while providing ‘future-proofing’ capabilities to support reanalyses using as yet unknown algorithms or approaches. Finally, this proposed RS reporting format is available online, and is intended to be a dynamic document, subject to further community feedback and/or change in the future.

Bond-Lamberty, Benjamin↗

Neutron scattering measurement of water content and chemical composition of alkali-glass powder reacted gel

Highlights: • Water absorption ability of alkali-glass powder reacted gel was largely reduced comparing other reported alkali-silica gels. • Both SAXS and SANS results showed similar internal structures of samples prepared with different H2O:D2O mixing ratios. • Neutron scattering method was developed to non-destructively determine gel water content and mass density of reacted gels. • Neutron scattering methods (based on scattering length density) on gel water content was validated with TGA measurement. - Abstract: Recent studies demonstrated that the added glass powder with high-alumina content could significantly reduce Alkali-Silica Reaction (ASR) damage in cement concrete. This paper aims to investigate the gel water content, chemical composition and expansion behavior of the alkali-glass powder reacted gel by using neutron scattering and other characterization techniques. Three types of samples were prepared with glass powder, sodium oxide and deuteroxide/hydrogen water with different molar ratios. The swelling potential of this alkali-glass powder reacted gel was much lower than that of the reported alkali-silica reacted gel. The gel water content and chemical compositions were characterized with small-angle neutron scattering (SANS) technique, supported by small-angle X-ray scattering (SAXS), prompt-gamma ray neutron activation analysis (PGAA) measurement. The SAXS test results showed the close scattering intensities, and thus similar internal microstructures among these samples. The elemental molar ratios of gels were obtained from the PGAA test results. Then the gel water molar ratio and mass density were determined by neutron scattering contrast calculation. The determined gel water content from neutron scattering analysis was validated with zero contrast analysis and TGA experimental measurement. Overall, this paper demonstrated the feasibility of using SANS technique to determine the water content of alkali-glass powder reacted gel.

36 MATERIALS SCIENCE↗

Measurement of the inclusive isolated prompt photon cross-section in pp collisions at root s=7 TeV using 35 pb(-1) of ATLAS data

A measurement of the differential cross-section for the inclusive production of isolated prompt photons in pp collisions at a center-of-mass energy {radical}s = 7 TeV is presented. The measurement covers the pseudorapidity ranges |{eta}| < 1.37 and 1.52 {le} |{eta}| < 2.37 in the transverse energy range 45 {le} E{sub T} < 400 GeV. The results are based on an integrated luminosity of 35 pb{sup -1}, collected with the ATLAS detector at the LHC. The yields of the signal photons are measured using a data-driven technique, based on the observed distribution of the hadronic energy in a narrow cone around the photon candidate and the photon selection criteria. The results are compared with next-to-leading order perturbative QCD calculations and found to be in good agreement over four orders of magnitude in cross-section.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Isolation of flow and nonflow correlations by two- and four-particle cumulant measurements of azimuthal harmonics in s NN = 200 GeV Au+Au collisions

A data-driven method was applied to Au+Au collisions at root S-NN = 200 GeV made with the STAR detector at RHIC to isolate pseudorapidity distance Delta eta-dependent and Delta eta-independent correlations by using two- and four-particle azimuthal cumulant measurements. We identified a Delta eta-independent component of the correlation, which is dominated by anisotropic flow and flow fluctuations. It was also found to be independent of. within the measured range of pseudorapidity vertical bar eta vertical bar < 1. In 20-30% central Au+Au collisions, the relative flow fluctuation was found to be 34% +/- 2%(stat.) +/- 3%(sys.) for particles with transverse momentum p(T) less than 2 GeV/c. The Delta eta-dependent part, attributed to nonflow correlations, is found to be 5% +/- 2%(sys.) relative to the flow of the measured second harmonic cumulant at vertical bar Delta eta vertical bar > 0.7. (C) 2015 The Authors. Published by Elsevier B.V.

74 ATOMIC AND MOLECULAR PHYSICS↗