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At least 613 records · Page 34

Designing Antifouling and Antimicrobial Interfaces: Structural Characterization using CryoEM, Automated Microscopy, and AI Image Segmentation

The design of functionalized surfaces for interactions with biological systems is critical across sectors such as healthcare, energy, and agriculture. Tailoring materials for specific applications, such as antifouling and antimicrobial surfaces, demands a comprehensive understanding of topology and chemistry across multiple length and time scales on both biological and materials systems. This work presents the development and characterization of nanostructured surfaces with controlled topographies and chemistries that enhance bacterial membrane disruption, reduce biofilm formation, and improve antimicrobial and antifouling capabilities. Two specific use cases will be presented - the use of cellulose nanocrystals (CNCs) for bacterial growth inhibition and the development of antifouling surfaces to prevent protein and bacterial adsorption [1-4]. By leveraging large language models (LLMs) for image segmentation and training [5], we enable automated analysis of terabyte-scale cryogenic electron microscopy (cryoEM) datasets. This analysis provides statistical insights into the biotic/abiotic interface and facilitates automated electron microscopy experiments to mitigate time and dose. The integration of cryogenic electron tomography (cryoET) and cryogenic focused ion beam (cryoFIB) milling enables high-resolution, near-native-state imaging and 3D reconstructions of bio/material interfaces [6]. Orthogonal characterization techniques and computational modeling further enhances our understanding, offering a robust platform for the design and optimization of next-generation functional surfaces [7].

Williams, Alexis [ORNL] (ORCID:0000000252835822)↗

Hyperspectral imaging of exciton confinement within a moiré unit cell with a subnanometer electron probe

Electronic and optical excitations in two-dimensional systems are distinctly sensitive to the presence of a moiré superlattice. Here we used cryogenic transmission electron microscopy and spectroscopy to simultaneously image the structural reconstruction and associated localization of the lowest-energy intralayer exciton in a rotationally aligned WS 2 -WSe 2 moiré superlattice. In conjunction with optical spectroscopy and ab initio calculations, we determined that the exciton center-of-mass wave function is confined to a radius of approximately 2 nanometers around the highest-energy stacking site in the moiré unit cell. Our results provide direct evidence that atomic reconstructions lead to the strongly confining moiré potentials and that engineering strain at the nanoscale will enable new types of excitonic lattices.

36 MATERIALS SCIENCE↗

An Integrated Multiscale Experimental-Numerical Analysis on Reconsolidation of Salt-Clay Mixture for Disposal of Heat-Generating Waste (Final NEUP Technical Report)

The overall purpose of this research is to improve understanding of THMC coupling effect on the reconsolidation of granular (or crushed) salt-clay mixture used for seal systems of shafts and drifts in salt repositories. This proposed work is partially motivated by the recent work on the Waste Isolation Pilot Plant (WIPP) that shows the promising sealing capability of clay-salt mixture compared to crushed salt. In particular, primary emphasis is to develop a fully integrated multiscale experiment-numerical study to determine and explain what leads to the superior sealing ability of the clay-salt mixture. These research activities are designed to seek further understanding of (1) why clay additives may enhance the fluid trapping and (2) whether this flow barrier effect may prevail under different combinations of temperature, confining pressure, deviatoric stress and other foreseeable environmental factors. If successful, this enhanced flow trapping ability of the seal provides significant improvement to the seal and repository performance and therefore make the repository safer in the long-term. The experiment component includes microstructural investigation and macroscopic tests on a reconsolidated salt-clay mixture. In the microstructural study, the goal is to (1) characterize microscopic distributions of distinct phases (e.g., clay, salt crystal boundaries, trapped brine, and pore) to examine the connectivity of the pore network inside the salt-clay mixture with different amounts of clay additive and moisture content and (2) analyze multiscale imaging data to reconstruct the polycrystalline microstructures for numerical simulations. Meanwhile, macroscopic tests are performed to analyze how clay alters the failure/creep mechanisms in the salt-clay mixture. Microscopic and macroscopic experimental observations will both be used to calibrate and validate a multiscale model that explicitly simulates the capillary and multiphase flow in the connected pores and the deformation due to the presence of intra-crystalline brine at the pore scale via a new polyhedral discrete element–lattice Boltzmann method (DEM-LBM) coupling model. The pore-scale simulations are homogenized via an upscaling procedure that converts pore-scale information (e.g. force exerted on grain boundary, sliding, pressure-solution) to continuum measures (e.g. Cauchy stress, Darcy’s flow) at each integration point in the macroscopic multiphase TMHC model. This multiscale scheme will allow coupling be- tween high-fidelity simulations of brine-salt-clay interaction and the macroscopic TMHC model. The multiscale model helps the understanding of how the trapped brine inclusion affects the pressure-solution mechanism with the presence of clay and moisture. This work brings new insight into the sealing capacity of salt-clay mixture under elevated temperature over a long period of time - a key to evaluating the potential of salt-clay mixture usage for salt repositories.

42 ENGINEERING↗

Evaluation of intracoronary hemodynamics identifies perturbations in vorticity

Coronary artery disease (CAD) is highly prevalent and associated with adverse events. Challenges have emerged in the treatment of intermediate coronary artery stenoses. These lesions are often interrogated with fractional flow reserve (FFR) testing to determine if a stenosis is likely to be causative for ischemia in a cardiac territory. This invasive test requires insertion of a pressure wire into a coronary vessel. Recently computational fluid dynamics (CFD) has been used to noninvasively assess fractional flow reserve in vessels reconstructed from medical imaging data. However, many of these simulations are unable to provide additional information about intravascular hemodynamics, including velocity, endothelial shear stress (ESS), and vorticity. We hypothesized that vorticity, which has demonstrated utility in the assessment of ventricular and aortic diseases, would also be an important hemodynamic factor in CAD. Three-dimensional (3D), patient-specific coronary artery geometries that included all vessels >1 mm in diameter were created from angiography data obtained from 10 patients who underwent diagnostic angiography and FFR testing (n = 9). A massively parallel CFD solver (HARVEY) was used to calculate coronary hemodynamic parameters including pressure, velocity, ESS, and vorticity. These simulations were validated by comparing velocity flow fields from simulation to both velocities derived from in vitro particle image velocimetry and to invasively acquired pressure wire-based data from clinical testing. There was strong agreement between findings from CFD simulations and particle image velocimetry experimental testing (p < 0.01). CFD-FFR was also highly correlated with invasively measured FFR (ρ = 0.77, p = 0.01) with an average error of 5.9 ± 0.1%. CFD-FFR also had a strong inverse correlation with the vorticity (ρ = -0.86, p = 0.001). Simulations to determine the effect of the coronary stenosis on intravascular hemodynamics demonstrated significant differences in velocity and vorticity (both p < 0.05). Further evaluation of an angiographically normal appearing non-FFR coronary vessel in patients with CAD also demonstrated differences in vorticity when compared with FFR vessels (p < 0.05). The use of highly accurate 3D CFD-derived intravascular hemodynamics provides additional information beyond pressure measurements that can be used to calculate FFR. Vorticity is one parameter that is modified by a coronary stenosis and appears to be abnormal in angiographically normal vessels in patients with CAD, highlighting a possible use-case in preventative screening for early coronary disease.

59 BASIC BIOLOGICAL SCIENCES↗

Heterodyne readout for read-write holographic memories

A heterodyne readout technique for read-write holographic memory systems that reconstruct a virtual image wavefront is described and demonstrated. The conventionally recorded hologram is illuminated simultaneously with a suitable combination of temporally modulated reference and modified object waves for readout. Best performance is obtained for temporal phase modulation. The coupling of the illuminating wavefronts by the hologram is analyzed.

Mezrich, R. S.↗

A statistical technique for processing radio interferometer data

The output of a radio interferometer is the Fourier transform of the object under investigation. Due to the limited coverage of the Fourier plane, the reconstruction of the image of the source is blurred by the beam of the synthesized array. A maximum-likelihood processing technique is described which uses the statistical properties of the received noise-like signals. This technique has been used extensively in the processing of large-aperture seismic arrays. This inversion method results in a synthesized beam that is more uniform, has lower sidelobes, and higher resolution than the normal Fourier transform methods. The maximum-likelihood method algorithm was applied successfully to very long baseline and short baseline interferometric data.

Papadopoulos, G. D.↗

Charge coupled device integration-time coding for detection of images moving with unknown velocities

Existing techniques for the detection of a moving low light level image by a CCD array have required velocity synchronism between the image and the photogenerated charges. This was necessary to prevent blurring during the long duration of charge integration. A new detection scheme is described which causes the image to be convolved with a clock modulation signal as the photocharges are collected. The charge accumulating from each image point will now be spread over many photoelements due to the absence of velocity synchronism, but the output is not blurred in the usual sense. Instead the charge is distributed through the array in a controlled way so that the image can be reconstructed.

White, J. M.↗

Mapping of lunar surface from side-looking orbital radar images

Side-looking spacecraft radar imagery has thus far been produced only from an orbit around the moon. This was a part of the Apollo Lunar Sounder Experiment (ALSE) of the Apollo 17 mission in December 1972. This paper reports results of a radargrammetric evaluation of overlapping Apollo 17 synthetic-aperture radar images (wavelength 2 m). The potential to map from single images and to reconstruct three-dimensional stereoscopic models is studied. The relative height accuracy achieved is about + or - 100 m and is thus competitive with that obtained with the vidicon camera presently used for planetary exploration.

Leberl, F.↗

An approach for automated analysis of particle holograms

A simple method for analyzing droplet holograms is proposed that is readily adaptable to automation using modern image digitizers and analyzers for determination of the number, location, and size distributions of spherical or nearly spherical droplets. The method determines these parameters by finding the spatial location of best focus of the droplet images. With this location known, the particle size may be determined by direct measurement of image area in the focal plane. Particle velocity and trajectory may be determined by comparison of image locations at different instants in time. The method is tested by analyzing digitized images from a reconstructed in-line hologram, and the results show that the method is more accurate than a time-consuming plane-by-plane search for sharpest focus.

Stanton, A. C.↗

Machine parts recognition using a trinary associative memory

The convergence mechanism of vectors in Hopfield's neural network in relation to recognition of partially known patterns is studied in terms of both inner products and Hamming distance. It has been shown that Hamming distance should not always be used in determining the convergence of vectors. Instead, inner product weighting coefficients play a more dominant role in certain data representations for determining the convergence mechanism. A trinary neuron representation for associative memory is found to be more effective for associative recall. Applications of the trinary associative memory to reconstruct machine part images that are partially missing are demonstrated by means of computer simulation as examples of the usefulness of this approach.

Awwal, Abdul Ahad S.↗

Image gathering and restoration - Information and visual quality

A method is investigated for optimizing the end-to-end performance of image gathering and restoration for visual quality. To achieve this objective, one must inevitably confront the problems that the visual quality of restored images depends on perceptual rather than mathematical considerations and that these considerations vary with the target, the application, and the observer. The method adopted in this paper is to optimize image gathering informationally and to restore images interactively to obtain the visually preferred trade-off among fidelity resolution, sharpness, and clarity. The results demonstrate that this method leads to significant improvements in the visual quality obtained by the traditional digital processing methods. These traditional methods allow a significant loss of visual quality to occur because they treat the design of the image-gathering system and the formulation of the image-restoration algorithm as two separate tasks and fail to account for the transformations between the continuous and the discrete representations in image gathering and reconstruction.

Mccormick, Judith A.↗

Mars Rover and Sample Return Mission design

The current reference Mars Rover and Sample Return mission is described. Technical issues are outlined, including high-resolution image acquisition and reconstruction, approach navigation, ground and flight systems operational complexity, rover autonomy, autonomous rendezvous and docking in Mars orbit, aerocapture and aeromaneuver, estimating the probability of mission success, and end-to-end information system design. Focus is placed on lander hazard identification and avoidance, pinpoint landing guidance and control, Mars ascent vehicle guidance and control, planetary protection and quarantine, sample acquisition and preservation, project management and control, systems requirements and interface control, and costing. In addition, program issues such as international participation, fiscal constraints, and launch-vehicle availability are considered.

Kwok, Johnny H.↗

Spectral eclipse mapping of the accretion disk in the nova-like variable UX Ursae Majoris

We analyze narrow-band eclipse light curves of the nova-like cataclysmic variable UX UMa, obtained from low-resolution spectra spanning lambda lambda 3600-9800 A . The light curves for narrow bands in the continuum as well as those for individual spectral lines are treated independently, and are used to construct images of the accretion disk's brightness distribution using the maximum-entropy eclipse-mapping technique. Particular attention is paid to the propagation of statistical uncertainties in the data and to how the analysis may introduce systematic errors in the final result. From the many narrrow band images we have reconstructed the spectra from isolated parts of the accretion disk. These spectra reveal that the inner disk radiates a continuum spectrum which peaks in the near UV and has the hydrogen Balmer lines in absorption (with the exception of H-alpha), whereas the outer disk is much fainter, has a much redder spectrum, and has Balmer emission lines. Our analysis reveals the presence of an uneclipsed component of the total light, whose spectrum is very red and has Balmer lines in emission. This unexpected feature of the eclipse mapping technique offers a new tool for an independent assessment of the secondary star's spectrum in eclipsing cataclysmic variables.

Rutten, R. G. M.↗

Computer Tomography Analysis of Fastrac Composite Thrust Chamber Assemblies

Computed tomography (CT) inspection has been integrated into the production process for NASA's Fastrac composite thrust chamber assemblies (TCAs). CT has been proven to be uniquely qualified to detect the known critical flaw for these nozzles, liner cracks that are adjacent to debonds between the liner and overwrap. CT is also being used as a process monitoring tool through analysis of low density indications in the nozzle overwraps. 3d reconstruction of CT images to produce models of flawed areas is being used to give program engineers better insight into the location and nature of nozzle flaws.

Beshears, Ronald D.↗

Advances in HgCdTe APDs and LADAR Receivers

Raytheon is developing NIR sensor chip assemblies (SCAs) for scanning and staring 3D LADAR systems. High sensitivity is obtained by integrating high performance detectors with gain i.e. APDs with very low noise Readout Integrated Circuits. Unique aspects of these designs include: independent acquisition (non-gated) of pulse returns, multiple pulse returns with both time and intensity reported to enable full 3D reconstruction of the image. Recent breakthrough in device design has resulted in HgCdTe APDs operating at 300K with essentially no excess noise to gains in excess of 100, low NEP <1nW and GHz bandwidths and have demonstrated linear mode photon counting. SCAs utilizing these high performance APDs have been integrated and demonstrated excellent spatial and range resolution enabling detailed 3D imagery both at short range and long ranges. In this presentation we will review progress in high resolution scanning, staring and ultra-high sensitivity photon counting LADAR sensors.

Bailey, Steven↗

Advances in LADAR Components and Subsystems at Raytheon

Raytheon is developing NIR sensor chip assemblies (SCAs) for scanning and staring 3D LADAR systems. High sensitivity is obtained by integrating high performance detectors with gain, i.e., APDs with very low noise Readout Integrated Circuits (ROICs). Unique aspects of these designs include: independent acquisition (non-gated) of pulse returns, multiple pulse returns with both time and intensity reported to enable full 3D reconstruction of the image. Recent breakthrough in device design has resulted in HgCdTe APDs operating at 300K with essentially no excess noise to gains in excess of 100, low NEP <1nW and GHz bandwidths and have demonstrated linear mode photon counting. SCAs utilizing these high performance APDs have been integrated and demonstrated excellent spatial and range resolution enabling detailed 3D imagery both at short range and long ranges. In the following we will review progress in real-time 3D LADAR imaging receiver products in three areas: (1) scanning 256 x 4 configuration for the Multi-Mode Sensor Seeker (MMSS) program and (2) staring 256 x 256 configuration for the Autonomous Landing and Hazard Avoidance Technology (ALHAT) lunar landing mission and (3) Photon-Counting SCAs which have demonstrated a dramatic reduction in dark count rate due to improved design, operation and processing.

Jack, Michael↗

Recent Advances in Soft Matter Characterization Capabilities Developed at NASA GRC for Lunar Exploration: Differential Dynamic Microscopy to Spectroscopy to Computer Vision

In 1991, famous French scientist Pierre-Gilles de Genes was awarded Nobel prize for his impactful research in soft matter, more specifically polymers. He is defined as the founding father of soft matter. In his Nobel lecture (https://www.nobelprize.org/uploads/2018/06/gennes-lecture.pdf ) he described soft matter aka complex fluids as materials with two primary features – (a) complexity and (b) flexibility. The sub-categories of soft matter (e.g.- granular materials, polymers, foams, colloids etc.) are defined on the basis of Pierre-Gilles de Gennes’ definition. At NASA GRC, we are pushing the boundaries for fundamental study of soft matter on Lunar Surface. With regard to Lunar surface science, we are focusing on developing capabilities pertaining to granular materials and bio-soft/active matter to facilitate future efforts in ISRU and bio-ISRU capabilities. In order to achieve fundamental goals of soft matter research within the limitations of Lunar environment, the scientific capabilities need to be small, flexible, modular, off the shelf and the focus needs to be more on developing an interdisciplinary capability that leverages the recent growth in AI/ML and Computer Vision to augment our understanding of fundamental science. This strategy would allow us to reduce our resource requirement during launch, installation, and occupied real estate footprint on Lunar surface In this talk, we will go over 3 different capabilities that we have developed in house and in close collaboration – (a) Differential Dynamic Microscopy (DDM), (b) Portable In-situ Chemical Spectroscopy (PICS) and (c) Computer Vision Enabled Observation. At very high level, Differential Dynamic Microscopy (DDM) allows us to study the structure-property-process relation (microrheology) of bio-soft/active matter using optical microscope and improved image analysis capabilities. PICS uses AI/ML-based advanced signal deconvolution and analysis technique that can work with existing portable spectroscopy tools to perform materials analysis (e.g.- granular materials and bio-soft/active matter) inspection on the go. Finally, computer vision enabled analysis allows us to use simple camera images for 3D reconstruction of experimental process and tracking of objects of interest in an experiment. We expect that this detailed process will allow us reach a thorough understanding of soft matter in Lunar environment. The capabilities developed by us will help to validate and establish fundamental understanding in Lunar environment. This will, in turn, allow us to guide future space exploration missions and expand the knowledge base of the scientific and engineering communities.

Suman Sinha Ray↗

Recent Advances in Soft Matter Characterization Capabilities Developed at NASA GRC for Lunar Exploration: Differential Dynamic Microscopy to Spectroscopy to Computer Vision

In 1991, famous French scientist Pierre-Gilles de Genes was awarded Nobel prize for his impactful research in soft matter, more specifically polymers. He is defined as the founding father of soft matter. In his Nobel lecture (https://www.nobelprize.org/uploads/2018/06/gennes-lecture.pdf ) he described soft matter aka complex fluids as materials with two primary features – (a) complexity and (b) flexibility. The sub-categories of soft matter (e.g.- granular materials, polymers, foams, colloids etc.) are defined on the basis of Pierre-Gilles de Gennes’ definition. At NASA GRC, we are pushing the boundaries for fundamental study of soft matter on Lunar Surface. With regard to Lunar surface science, we are focusing on developing capabilities pertaining to granular materials and bio-soft/active matter to facilitate future efforts in ISRU and bio-ISRU capabilities. In order to achieve fundamental goals of soft matter research within the limitations of Lunar environment, the scientific capabilities need to be small, flexible, modular, off the shelf and the focus needs to be more on developing an interdisciplinary capability that leverages the recent growth in AI/ML and Computer Vision to augment our understanding of fundamental science. This strategy would allow us to reduce our resource requirement during launch, installation, and occupied real estate footprint on Lunar surface. In this talk, we will go over 3 different capabilities that we have developed in house and in close collaboration – (a) Differential Dynamic Microscopy (DDM), (b) Portable In-situ Chemical Spectroscopy (PICS) and (c) Computer Vision Enabled Observation. At very high level, Differential Dynamic Microscopy (DDM) allows us to study the structure-property-process relation (microrheology) of bio-soft/active matter using optical microscope and improved image analysis capabilities. PICS uses AI/ML-based advanced signal deconvolution and analysis technique that can work with existing portable spectroscopy tools to perform materials analysis (e.g.- granular materials and bio-soft/active matter) inspection on the go. Finally, computer vision enabled analysis allows us to use simple camera images for 3D reconstruction of experimental process and tracking of objects of interest in an experiment. We expect that this detailed process will allow us reach a thorough understanding of soft matter in Lunar environment. The capabilities developed by us will help to validate and establish fundamental understanding in Lunar environment. This will, in turn, allow us to guide future space exploration missions and expand the knowledge base of the scientific and engineering communities.

Suman Sinha-Ray↗