Engineering Papers⌕ Search

SEARCH · Engineering Papers

Results for “ESCAPE”

Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 613 records · Page 34

Halo mass dependence of H I and O VI absorption: evidence for differential kinematics

We studied a sample of 14 galaxies (0.1 < z < 0.7) using HST/WFPC2 imaging and high-resolution HST/COS or HST/STIS quasar spectroscopy of Lyα, Lyβ, and O VI λλ1031, 1037 absorption. The galaxies, having 10.8 ≤ log (M {sub h}/M {sub ☉}) ≤ 12.2, lie within D = 300 kpc of quasar sightlines, probing out to D/R {sub vir} = 3. When the full range of M {sub h} and D/R {sub vir} of the sample are examined, ∼40% of the H I absorbing clouds can be inferred to be escaping their host halo. The fraction of bound clouds decreases as D/R {sub vir} increases such that the escaping fraction is ∼15% for D/R {sub vir} < 1, ∼45% for 1 ≤ D/R {sub vir} < 2, and ∼90% for 2 ≤ D/R {sub vir} < 3. Adopting the median mass log M {sub h}/M {sub ☉} = 11.5 to divide the sample into 'higher' and 'lower' mass galaxies, we find a mass dependency for the hot circumgalactic medium kinematics. To our survey limits, O VI absorption is found in only ∼40% of the H I clouds in and around lower mass halos as compared to ∼85% around higher mass halos. For D/R {sub vir} < 1, lower mass halos have an escape fraction of ∼65%, whereas higher mass halos have an escape fraction of ∼5%. For 1 ≤ D/R {sub vir} < 2, the escape fractions are ∼55% and ∼35% for lower mass and higher mass halos, respectively. For 2 ≤ D/R {sub vir} < 3, the escape fraction for lower mass halos is ∼90%. We show that it is highly likely that the absorbing clouds reside within 4R {sub vir} of their host galaxies and that the kinematics are dominated by outflows. Our finding of 'differential kinematics' is consistent with the scenario of 'differential wind recycling' proposed by Oppenheimer et al. We discuss the implications for galaxy evolution, the stellar to halo mass function, and the mass-metallicity relationship of galaxies.

79 ASTRONOMY AND ASTROPHYSICS↗

INFERRING GRAVITATIONAL POTENTIALS FROM MASS DENSITIES IN CLUSTER-SIZED HALOS

We use N-body simulations to quantify how the escape velocity in cluster-sized halos maps to the gravitational potential in a ΛCDM universe. Using spherical density-potential pairs and the Poisson equation, we find that the matter density inferred gravitational potential profile predicts the escape velocity profile to within a few percent accuracy for group and cluster-sized halos (10{sup 13}<M{sub 200}<10{sup 15} M {sub ⊙}, with respect to the critical density). The accuracy holds from just outside the core to beyond the virial radius. We show the importance of explicitly incorporating a cosmological constant when inferring the potential from the Poisson equation. We consider three density models and find that the Einasto and Gamma profiles provide a better joint estimate of the density and potential profiles than the Navarro, Frenk, and White profile, which fails to accurately represent the escape velocity. For individual halos, the 1σ scatter between the measured escape velocity and the density-inferred potential profile is small (<5%). Finally, while the sub-halos show 15% biases in their representation of the particle velocity dispersion profile, the sub-halo escape velocity profile matches the dark matter escape velocity profile to high accuracy with no evidence of velocity bias outside 0.4r {sub 200}.

79 ASTRONOMY AND ASTROPHYSICS↗

Flux Sensitivity Requirements for the Detection of Lyman Continuum Radiation Drop-ins from Star-forming Galaxies below Redshifts of 3

Flux estimates for ionizing radiation escaping from star-forming galaxies with characteristic UV luminosities (L{sub 1500∗(1+z)}{sup ∗}), derived from GALEX and the VIMOS-VLT Deep Survey, are presented as a function of redshift and assumed escape fraction. These estimates offer guidance to the design of instrumentation and observing strategies, be they spectroscopic or photometric, for attempting to detect LyC escaping star-forming galaxies for redshifts z<3. Examples are given that relate the integrated escape fraction (f {sup e} {sub LyC}) of ionizing photons, obtained by integrating over the entire extreme UV (EUV) bandpass, to the relative escape fraction (f {sup e} {sub 900}) observed just shortward of the ionization edge at 911.8 Å as a function of H i, He i, and He ii column densities. We find that for logN{sub HI}(cm{sup −2})≳17.0, f {sup e} {sub LyC} is significantly greater than f {sup e} {sub 900}. Detection of LyC “drop-ins” in the rest-frame EUV will provide enhanced fidelity to determinations of the integrated fraction of ionizing photons f {sup e} {sub LyC} that escape star-forming galaxies and contribute to the metagalactic ionizing background (MIB).

79 ASTRONOMY AND ASTROPHYSICS↗

Triton's atmosphere - A source of N and H for Neptune's magnetosphere

Mass loading of the Neptunian magnetosphere occurs primarily by thermal escape of H, H2, and N from Triton's upper atmosphere. The global escape rate of hydrogen is about 7 x 10 exp 25/s, determined by the global average methane photolysis rate, whereas the escape rate of nitrogen for the present preferred model is about 3.4 x 10 exp 25/s, and is controlled by the global and orbital average energy deposition rate due to precipitating magnetospheric electrons. The escape rate of H(+) and N(+) is less than 4 percent of the neutral escape rate and implies that mass loading of the Neptunian magnetosphere is not localized to Triton's corona. The ratio of hydrogen to nitrogen escape rates for the present preferred model is about 2:1, comparable to the H(+)/N(+) abundance ratio inferred for Neptune's magnetosphere.

Summers, Michael E.↗

Elemental Fractionation During Rapid Accretion of the Moon Triggered by a Giant Impact

Recently, Ida et al. made an N-body simulation of lunar accretion from a protolunar disk formed by a giant impact. One of their important conclusions is that the accretion time of the Moon is as short as one month. Such rapid accretion is a necessary consequence of the high surface density of a lunar mass disk accreting just beyond the Roche limit (about 3Re); the Safronov accretion time (a few days) is even shorter. The energy of accretion always exceeds the gravitational binding energy of newly arriving matter. Hence, without an energy sink, the accreting body is thermally unstable. For the Earth and other planets, radiation acts as the sink. However, in such a short accretion time, the Moon cannot radiate the accretional energy. Even radiating at a silicate cloudtop temperature of roughly 2000 K, it would take more than 100 yr to radiatively cool the Moon. The plausible alternative heat sinks are heat capacity, latent heat of vaporization, and thermal escape of the gas to space (i.e., hydrodynamic blowoff). The latter becomes plausible for the Moon because the scale height at 2000 K (about 300 km) is a significant fraction of the lunar radius. The early stages of lunar (or "lunatesimal") growth release relatively little energy and can occur simply by heating the material, especially if the accreting material is originally cold. However, the material is unlikely to be cold, because the disk itself is hot and cooling time is long, while the lunar accretion time iss very short. Therefore, the moon is likely to accrete condensed material just after it condenses. Accordingly, the newly accreted material will be on the verge of vaporization and will have very little heat capacity to spare. The immediate heat sink is the latent heat of vaporization. Most of the vapor will escape from the moon, because the thermal energy in the gas can be used to drive escape. However, vaporization is generally incomplete. the latent heat of vaporization exceeds the energy of accretion. Viewed globally, the accretional energy is about half the energy required to vaporize the entire Moon. Thus to first approximation, half of the Moon-forming material can be vaporized and lost during accretion. During this process, we would expect preferential loss of relatively volatile elements. Escape will retard the rate of accretion. To test these ideas, we computed detailed models of the thermal state of the Moon during accretion. We pay special attention to the structure of the silicate atmosphere and its loss rate by calculating the chemical species at equilibrium. We used the PHEQ program which includes 12 elements (H,O,C,Mg,Si,Fe,Ca, Al, Na,Ti, and N.) and 272 compounds (including ionic compounds). Because of the large heats of vaporization and ionization, the adiabatic atmosphere is nearly isothermal and massive escape is expected. The pressure of the atmosphere is determined by the balance between vaporization of a accreting material and escape. If the accretion time is one month, a 0.3 bar atmosphere is expected. Elemental fractionation depends strongly on the temperature of the accreting material. The initial temperature of the material can be estimated from the condition of gravitational instability in the protolunar disk. As shown by Ida et al, accretion starts when gravitational instability occurs when more than 99% of the material condenses. At this point, all of Ca, Al, Si, Mg, and Fe, and 95% of Na (probably K also), are in condensed phases. If the moon is formed from the accretion of such material, volatile elements such as Na, and K are retained by the moon only early in accretion. At later times, K and Na are lost and a fraction of the MG, Si and Fe is lost. However, refractory elements such as Ca and Al are retained and so achieve a mild degree (factor 2) of superabundance.

Abe, Y.↗

A Spectroscopic Search for Leaking Lyman Continuum at Zeta Approximately 0.7

We present the results of rest-frame, UV slitless spectroscopic observations of a sample of 32 z approx. 0.7 Lyman Break Galaxy (LBG) analogs in the COSMOS field. The spectroscopic search was performed with the Solar Blind Channel (SBC) on HST. While we find no direct detections of the Lyman Continuum we achieve individual limits (3sigma) of the observed non-ionizing UV to Lyman continuum flux density ratios, f(sub nu)(1500A)/f(sub nu)(830A) of 20 to 204 (median of 73.5) and 378.7 for the stack. Assuming an intrinsic Lyman Break of 3.4 and an optical depth of Lyman continuum photons along the line of sight to the galaxy of 85% we report an upper limit for the relative escape fraction in individual galaxies of 0.02 - 0.19 and a stacked 3sigma upper limit of 0.01. We find no indication of a relative escape fraction near unity as seen in some LBGs at z approx. 3. Our UV spectra achieve the deepest limits to date at any redshift on the escape fraction in individual sources. The contrast between these z approx. 0.7 low escape fraction LBG analogs with z approx. 3 LBGs suggests that either the processes conducive to high f(sub esc) are not being selected for in the z less than or approx.1 samples or the average escape fraction is decreasing from z approx. 3 to z approx. 1. We discuss possible mechanisms which could affect the escape of Lyman continuum photons

Bridge, Carrie R.↗

A Proposed Ascent Abort Flight Test for the Max Launch Abort System

The NASA Engineering and Safety Center initiated the Max Launch Abort System (MLAS) Project to investigate alternate crew escape system concepts that eliminate the conventional launch escape tower by integrating the escape system into an aerodynamic fairing that fully encapsulates the crew capsule and smoothly integrates with the launch vehicle. This paper proposes an ascent abort flight test for an all-propulsive towerless escape system concept that is actively controlled and sized to accommodate the Orion Crew Module. The goal of the flight test is to demonstrate a high dynamic pressure escape and to characterize jet interaction effects during operation of the attitude control thrusters at transonic and supersonic conditions. The flight-test vehicle is delivered to the required test conditions by a booster configuration selected to meet cost, manufacturability, and operability objectives. Data return is augmented through judicious design of the boost trajectory, which is optimized to obtain data at a range of relevant points, rather than just a single flight condition. Secondary flight objectives are included after the escape to obtain aerodynamic damping data for the crew module and to perform a high-altitude contingency deployment of the drogue parachutes. Both 3- and 6-degree-of-freedom trajectory simulation results are presented that establish concept feasibility, and a Monte Carlo uncertainty assessment is performed to provide confidence that test objectives can be met.

Tartabini, Paul V.↗

Computationally restoring the potency of a clinical antibody against Omicron

The COVID-19 pandemic underscored the promise of monoclonal antibody-based prophylactic and therapeutic drugs and revealed how quickly viral escape can curtail effective options. When the SARS-CoV-2 Omicron variant emerged in 2021, many antibody drug products lost potency, including Evusheld and its constituent, cilgavimab. Cilgavimab, like its progenitor COV2-2130, is a class 3 antibody that is compatible with other antibodies in combination4 and is challenging to replace with existing approaches. Rapidly modifying such high-value antibodies to restore efficacy against emerging variants is a compelling mitigation strategy. We sought to redesign and renew the efficacy of COV2-2130 against Omicron BA.1 and BA.1.1 strains while maintaining efficacy against the dominant Delta variant. Here we show that our computationally redesigned antibody, 2130-1-0114-112, achieves this objective, simultaneously increases neutralization potency against Delta and subsequent variants of concern, and provides protection in vivo against the strains tested: WA1/2020, BA.1.1 and BA.5. Deep mutational scanning of tens of thousands of pseudovirus variants reveals that 2130-1-0114-112 improves broad potency without increasing escape liabilities. Our results suggest that computational approaches can optimize an antibody to target multiple escape variants, while simultaneously enriching potency. Our computational approach does not require experimental iterations or pre-existing binding data, thus enabling rapid response strategies to address escape variants or lessen escape vulnerabilities.

60 APPLIED LIFE SCIENCES↗

Confinement of electrons in the focus of the dipole wave

Based on the numerical modelling of the electron dynamics in the focal region of a dipole wave, we have found the confinement time of electrons in the region of the strongest electric field in a wide (up to 200 PW) power range. By comparing confinement times of particles and the spatial structures of their escape, we have determined the regimes of ponderomotive escape and radiation-dominated escape corresponding to normal and anomalous radiative trapping, as well as the threshold powers of these regimes. It is shown that in the regimes of ponderomotive escape, the confinement time decreases with increasing power and stabilises at the level of one third of the wave period with the particles leaving the focal region predominantly across the electric field. In the radiation-dominated regime, the confinement time, on the contrary, increases with increasing power, the rate of transverse particles’ escape decreases, and the particles leave the focal region in the form of compact beams. (paper)

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

THE PHYSICS OF THE FAR-INFRARED-RADIO CORRELATION. I. CALORIMETRY, CONSPIRACY, AND IMPLICATIONS

The far-infrared (FIR) and radio luminosities of star-forming galaxies are linearly correlated over a very wide range in star formation rate, from normal spirals like the Milky Way to the most intense starbursts. Using one-zone models of cosmic ray (CR) injection, cooling, and escape in star-forming galaxies, we attempt to reproduce the observed FIR-radio correlation (FRC) over its entire span. The normalization and linearity of the FRC, together with constraints on the CR population in the Milky Way, have strong implications for the CR and magnetic energy densities in star-forming galaxies. We show that for consistency with the FRC, {approx}2% of the kinetic energy from supernova explosions must go into high-energy primary CR electrons and that {approx}10%-20% must go into high-energy primary CR protons. Secondary electrons and positrons are likely comparable to or dominate primary electrons in dense starburst galaxies. We discuss the implications of our models for the magnetic field strengths of starbursts, the detectability of starbursts by Fermi, and CR feedback. Overall, our models indicate that both CR protons and electrons escape from low surface density galaxies, but lose most of their energy before escaping dense starbursts. The FRC is caused by a combination of the efficient cooling of CR electrons (calorimetry) in starbursts and a conspiracy of several factors. For lower surface density galaxies, the decreasing radio emission caused by CR escape is balanced by the decreasing FIR emission caused by the low effective UV dust opacity. In starbursts, bremsstrahlung, ionization, and inverse Compton cooling decrease the radio emission, but they are countered by secondary electrons/positrons and the dependence of synchrotron frequency on energy, both of which increase the radio emission. Our conclusions hold for a broad range of variations in our fiducial model, such as those including winds, different magnetic field strengths, and different diffusive escape times.

79 ASTRONOMY AND ASTROPHYSICS↗

A SYSTEMATIC ANALYSIS OF CAUSTIC METHODS FOR GALAXY CLUSTER MASSES

We quantify the expected observed statistical and systematic uncertainties of the escape velocity as a measure of the gravitational potential and total mass of galaxy clusters. We focus our attention on low redshift (z {<=}0.15) clusters, where large and deep spectroscopic datasets currently exist. Utilizing a suite of Millennium Simulation semi-analytic galaxy catalogs, we find that the dynamical mass, as traced by either the virial relation or the escape velocity, is robust to variations in how dynamical friction is applied to ''orphan'' galaxies in the mock catalogs (i.e., those galaxies whose dark matter halos have fallen below the resolution limit). We find that the caustic technique recovers the known halo masses (M{sub 200}) with a third less scatter compared to the virial masses. The bias we measure increases quickly as the number of galaxies used decreases. For N{sub gal} > 25, the scatter in the escape velocity mass is dominated by projections along the line-of-sight. Algorithmic uncertainties from the determination of the projected escape velocity profile are negligible. We quantify how target selection based on magnitude, color, and projected radial separation can induce small additional biases into the escape velocity masses. Using N{sub gal} = 150 (25), the caustic technique has a per cluster scatter in ln (M|M{sub 200}) of 0.3 (0.5) and bias 1% {+-} 3{r_brace} (16% {+-} 5{r_brace}) for clusters with masses >10{sup 14} M{sub Sun} at z < 0.15.

79 ASTRONOMY AND ASTROPHYSICS↗

DETERMINATION OF STOCHASTIC ACCELERATION MODEL CHARACTERISTICS IN SOLAR FLARES

Following our recent paper, we have developed an inversion method to determine the basic characteristics of the particle acceleration mechanism directly and non-parametrically from observations under the leaky box framework. Earlier, we demonstrated this method for obtaining the energy dependences of the escape time and pitch angle scattering time. Here, by converting the Fokker-Planck equation to its integral form, we derive the energy dependences of the energy diffusion coefficient and direct acceleration rate for stochastic acceleration in terms of the accelerated and escaping particle spectra. Combining the regularized inversion method of Piana et al. and our procedure, we relate the acceleration characteristics in solar flares directly to the count visibility data from RHESSI. We determine the timescales for electron escape, pitch angle scattering, energy diffusion, and direct acceleration at the loop top acceleration region for two intense solar flares based on the regularized electron flux spectral images. The X3.9 class event shows dramatically different energy dependences for the acceleration and scattering timescales, while the M2.1 class event shows a milder difference. The discrepancy between the M2.1 class event and the stochastic acceleration model could be alleviated by a turbulence spectrum that is much steeper than the Kolmogorov-type spectrum. A likely explanation of the X3.9 class event could be that the escape of electrons from the acceleration region is not governed by a random walk process, but instead is affected by magnetic mirroring, in which the scattering time is proportional to the escape time and has an energy dependence similar to the energy diffusion time.

79 ASTRONOMY AND ASTROPHYSICS↗

HELIUM ATMOSPHERES ON WARM NEPTUNE- AND SUB-NEPTUNE-SIZED EXOPLANETS AND APPLICATIONS TO GJ 436b

Warm Neptune- and sub-Neptune-sized exoplanets in orbits smaller than Mercury’s are thought to have experienced extensive atmospheric evolution. Here we propose that a potential outcome of this atmospheric evolution is the formation of helium-dominated atmospheres. The hydrodynamic escape rates of Neptune- and sub-Neptune-sized exoplanets are comparable to the diffusion-limited escape rate of hydrogen, and therefore the escape is heavily affected by diffusive separation between hydrogen and helium. A helium atmosphere can thus be formed—from a primordial hydrogen–helium atmosphere—via atmospheric hydrodynamic escape from the planet. The helium atmosphere has very different abundances of major carbon and oxygen species from those of a hydrogen atmosphere, leading to distinctive transmission and thermal emission spectral features. In particular, the hypothesis of a helium-dominated atmosphere can explain the thermal emission spectrum of GJ 436b, a warm Neptune-sized exoplanet, while also being consistent with the transmission spectrum. This model atmosphere contains trace amounts of hydrogen, carbon, and oxygen, with the predominance of CO over CH{sub 4} as the main form of carbon. With our atmospheric evolution model, we find that if the mass of the initial atmosphere envelope is 10{sup −3} planetary mass, hydrodynamic escape can reduce the hydrogen abundance in the atmosphere by several orders of magnitude in ∼10 billion years. Observations of exoplanet transits may thus detect signatures of helium atmospheres and probe the evolutionary history of small exoplanets.

79 ASTRONOMY AND ASTROPHYSICS↗

Lyα EMISSION FROM GREEN PEAS: THE ROLE OF CIRCUMGALACTIC GAS DENSITY, COVERING, AND KINEMATICS

We report Hubble Space Telescope/Cosmic Origins Spectrograph observations of the Lyα emission and interstellar absorption lines in a sample of 10 star-forming galaxies at z ∼ 0.2. Selected on the basis of high equivalent width optical emission lines, the sample, dubbed “Green Peas,” make some of the best analogs for young galaxies in an early universe. We detect Lyα emission in all ten galaxies, and 9/10 show double-peaked line profiles suggestive of low H i column density. We measure Lyα/Hα flux ratios of 0.5–5.6, implying that 5%–60% of Lyα photons escape the galaxies. These data confirm previous findings that low-ionization metal absorption (LIS) lines are weaker when Lyα escape fraction and equivalent width are higher. However, contrary to previously favored interpretations of this trend, increased Lyα output cannot be the result of a varying H i covering: the Lyman absorption lines (Lyβ and higher) show a covering fraction near unity for gas with N{sub H} {sub i} ≳ 10{sup 16} cm{sup −2}. Moreover, we detect no correlation between Lyα escape and the outflow velocity of the LIS lines, suggesting that kinematic effects do not explain the range of Lyα/Hα flux ratios in these galaxies. In contrast, we detect a strong anticorrelation between the Lyα escape fraction and the velocity separation of the Lyα emission peaks, driven primarily by the velocity of the blue peak. As this velocity separation is sensitive to H i column density, we conclude that Lyα escape in these Green Peas is likely regulated by the H i column density rather than outflow velocity or H i covering fraction.

79 ASTRONOMY AND ASTROPHYSICS↗

VOLATILE LOSS AND CLASSIFICATION OF KUIPER BELT OBJECTS

Observations indicate that some of the largest Kuiper Belt Objects (KBOs) have retained volatiles in the gas phase (e.g., Pluto), while others have surface volatiles that might support a seasonal atmosphere (e.g., Eris). Since the presence of an atmosphere can affect their reflectance spectra and thermal balance, Schaller and Brown examined the role of volatile escape driven by solar heating of the surface. Guided by recent simulations, we estimate the loss of primordial N{sub 2} for several large KBOs, accounting for escape driven by UV/EUV heating of the upper atmosphere as well as by solar heating of the surface. For the latter we present new simulations and for the former we scale recent detailed simulations of escape from Pluto using the energy limited escape model validated recently by molecular kinetic simulations. Unlike what has been assumed to date, we show that unless the N{sub 2} atmosphere is thin (<∼10{sup 18} N{sub 2} cm{sup −2}) and/or the radius small (<∼200–300 km), escape is primarily driven by the UV/EUV radiation absorbed in the upper atmosphere. This affects the discussion of the relationship between atmospheric loss and the observed surface properties for a number of the KBOs examined. Our long-term goal is to connect detailed atmospheric loss simulations with a model for volatile transport for individual KBOs.

79 ASTRONOMY AND ASTROPHYSICS↗

Ionizing radiation from AGNs at z > 3.3 with the Subaru Hyper Suprime-Cam Survey and the CFHT Large Area U-band Deep Survey (CLAUDS)

ABSTRACT We use deep and wide imaging data from the CFHT Large Area U-band Deep Survey (CLAUDS) and the Hyper Suprime-Cam Subaru Strategic Program (HSC-SSP) to constrain the ionizing radiation (Lyman Continuum, LyC) escape fraction from active galactic nuclei (AGNs) at z ∼ 3–4. For 94 AGNs with spectroscopic redshifts at 3.3 < z < 4.0, we use their U/i-band flux ratios to estimate LyC transmission of individual AGNs. The distribution of their LyC transmission shows values lower than the range of LyC transmission values for IGM of the same redshift range, which suggests that LyC escape fraction of AGNs at z > 3.3 is considerably lower than unity in most cases. We do not find any trend in LyC transmission values depending on their UV luminosities. Based on the photometry of stacked images we find the average flux ratio of LyC and non-ionizing UV photons escaping from the objects (fLyC/fUV)out = 0.182 ± 0.043 for AGNs at 3.3 < z < 3.6, which corresponds to LyC escape fraction fesc = 0.303 ± 0.072 if we assume a fiducial intrinsic SED of AGN. Based on the estimated LyC escape fraction and the UV luminosity function (UVLF) of AGNs, we argue that UV-selected AGNs’ contribution to the LyC emissivity at the epoch is minor, although the size of their contribution largely depends on the shape of the UVLF.

79 ASTRONOMY AND ASTROPHYSICS↗

Rational optimization of tolC as a powerful dual selectable marker for genome engineering

Selection has been invaluable for genetic manipulation, although counter-selection has historically exhibited limited robustness and convenience. TolC, an outer membrane pore involved in transmembrane transport in E. coli, has been implemented as a selectable/counter-selectable marker, but counter-selection escape frequency using colicin E1 precludes using tolC for inefficient genetic manipulations and/or with large libraries. Here, we leveraged unbiased deep sequencing of 96 independent lineages exhibiting counter-selection escape to identify loss-of-function mutations, which offered mechanistic insight and guided strain engineering to reduce counter-selection escape frequency by ~40-fold. We fundamentally improved the tolC counter-selection by supplementing a second agent, vancomycin, which reduces counter-selection escape by 425-fold, compared colicin E1 alone. Combining these improvements in a mismatch repair proficient strain reduced counter-selection escape frequency by 1.3E6-fold in total, making tolC counter-selection as effective as most selectable markers, and adding a valuable tool to the genome editing toolbox. These improvements permitted us to perform stable and continuous rounds of selection/counter-selection using tolC, enabling replacement of 10 alleles without requiring genotypic screening for the first time. Finally, we combined these advances to create an optimized E. coli strain for genome engineering that is ~10-fold more efficient at achieving allelic diversity than previous best practices.

59 BASIC BIOLOGICAL SCIENCES↗

Hanford Reach Fall Chinook Salmon Productivity Analysis, Brood Years 1975–2016

Updating the Hanford Reach fall Chinook salmon productivity analysis to include seven additional years of data (BY 2010–2016), including three years of record escapements (>150,000 adults), reaffirmed the highly productive nature of this population and the positive effect of flow fluctuation constraints as implemented at Priest Rapids Dam by the VBSA and HRFCPP. The addition of several years of high escapements also revealed that competition among juveniles (and not spawning habitat limitations) is likely the mechanism driving density dependence in the Hanford Reach. The addition of the high escapement brood years also provided evidence for the high recruitment capacity of the population. As such, the WDFW may consider increasing the maximum escapement goal from 42,000 adults to 49,000 adults, which would be more in-line with SMSY as estimated from this update. Given the in-river protections that are in place as part of the HRFCPP, ocean conditions appear to be the primary factor controlling adult production. Therefore, the success of any additional management actions taken in freshwater are likely to be dependent on survival in the ocean. As such, the most effective management actions may be those that limit juvenile competition and density dependent growth effects in the Hanford Reach. Limiting competition would be expected to increase growth, resulting in larger smolts that would be less likely to suffer size-dependent mortality in the marine environment, even during years of poor ocean conditions. Increasing terminal harvest rates when high escapements are anticipated (from downstream dam counts) is one management option that could help to curtail juvenile competition.

Harnish, Ryan A.↗