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At least 595 records · Page 33

Use of virus‐induced gene silencing to characterize genes involved in modulating hypersensitive cell death in maize

Abstract Plant disease resistance proteins (R‐proteins) detect specific pathogen‐derived molecules, triggering a defence response often including a rapid localized cell death at the point of pathogen penetration called the hypersensitive response (HR). The maize Rp1‐D21 gene encodes a protein that triggers a spontaneous HR causing spots on leaves in the absence of any pathogen. Previously, we used fine mapping and functional analysis in a Nicotiana benthamiana transient expression system to identify and characterize a number of genes associated with variation in Rp1‐D21 ‐induced HR. Here we describe a system for characterizing genes mediating HR, using virus‐induced gene silencing (VIGS) in a maize line carrying Rp1‐D21 . We assess the roles of 12 candidate genes. Three of these genes, SGT1 , RAR1 , and HSP90 , are required for HR induced by a number of R‐proteins across several plant–pathogen systems. We confirmed that maize HSP90 was required for full Rp1‐D21 ‐induced HR. However, suppression of SGT1 expression unexpectedly increased the severity of Rp1‐D21 ‐induced HR while suppression of RAR1 expression had no measurable effect. We confirmed the effects on HR of two genes we had previously validated in the N. benthamiana system, hydroxycinnamoyltransferase and caffeoyl CoA O‐methyltransferase . We further showed the suppression the expression of two previously uncharacterized, candidate genes, IQ calmodulin binding protein ( IQM3 ) and vacuolar protein sorting protein 37 , suppressed Rp1‐D21 ‐induced HR. This approach is an efficient way to characterize the roles of genes modulating the hypersensitive defence response and other dominant lesion phenotypes in maize.

59 BASIC BIOLOGICAL SCIENCES↗

Covalent inhibition of hAChE by organophosphates causes homodimer dissociation through long-range allosteric effects

Acetylcholinesterase (EC 3.1.1.7), a key acetylcholine-hydrolyzing enzyme in cholinergic neurotransmission, is present in a variety of states in situ, including monomers, C-terminally disulfide-linked homodimers, homotetramers, and up to three tetramers covalently attached to structural subunits. Could oligomerization that ensures high local concentrations of catalytic sites necessary for efficient neurotransmission be affected by environmental factors? Using small-angle X-ray scattering (SAXS) and cryo-EM, we demonstrate that homodimerization of recombinant monomeric human acetylcholinesterase (hAChE) in solution occurs through a C-terminal four-helix bundle at micromolar concentrations. We show that diethylphosphorylation of the active serine in the catalytic gorge or isopropylmethylphosphonylation by the R P enantiomer of sarin promotes a 10-fold increase in homodimer dissociation. We also demonstrate the dissociation of organophosphate (OP)-conjugated dimers is reversed by structurally diverse oximes 2PAM, HI6, or RS194B, as demonstrated by SAXS of diethylphosphoryl-hAChE. However, binding of oximes to the native ligand-free hAChE, binding of high-affinity reversible ligands, or formation of an S P -sarin-hAChE conjugate had no effect on homodimerization. Dissociation monitored by time-resolved SAXS occurs in milliseconds, consistent with rates of hAChE covalent inhibition. OP-induced dissociation was not observed in the SAXS profiles of the double-mutant Y337A/F338A, where the active center gorge volume is larger than in wildtype hAChE. These observations suggest a key role of the tightly packed acyl pocket in allosterically triggered OP-induced dimer dissociation, with the potential for local reduction of acetylcholine-hydrolytic power in situ. Computational models predict allosteric correlated motions extending from the acyl pocket toward the four-helix bundle dimerization interface 25 Å away.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Controlled neoclassical tearing mode (NTM) healing by fueling pellets and its impact on electron cyclotron current drive requirements for complete NTM stabilization

Controlled partial stabilization of core m/n – 2/1 Neoclassical Tearing Modes (NTMs) by fueling deuterium pellets is reported in DIII-D and KSTAR H-mode plasmas (m=n are the poloidal/toroidal mode numbers). Analyses of DIII-D data exploring possible physics origins show that an explanation is offered by NTM-turbulence multi-scale interaction, triggered by a sudden increase of local gradients near q–2 caused by the pellet. Pellet injection from the high-field side allows deep fueling which reaches the island region. In turn, low-k turbulent density fluctuations (ñ) increase by 30% in the island region. This ñ can drive transport across the island separatrices, reducing the pressure at spot at the O-point and diminishing the NTM drive. The Mirnov probe array detects the reduction of the 2/1 magnetic amplitude by up to 20%. Causality between elevated gradients outside of the island, turbulence spreading into the island and reduced NTM drive is qualitatively supported by non-linear gyrokinetic turbulence simulations. These demonstrate increased penetration of ion-scale ñ from the background plasma to the O-point region when the background gradient is increased. This interaction has potentially far reaching consequences as it can can lead to a reduction of the required electron cyclotron current density (j ECCD ) for NTM suppression by 70%, as predicted by the modified Rutherford equation. Furthermore, this beneicial effect of fueling pellets can be important as j ECCD is the anticipated active NTM control technique for the International Thermonuclear Experimental Reactor (ITER), but its efficiency will be lowered by third harmonic absorption in Pre-Fusion Power Operation-1 (PFPO-1) at half magnetic field.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

The 3D magnetic topology and plasma dynamics in open stochastic magnetic field lines

We report the thermal quench triggered by locked modes is known to be mainly due to open stochastic magnetic field lines connected to the wall boundary. It is essential to understand the 3D structure of open stochastic field lines since it determines the overall plasma dynamics in the system. In this study, we analyze the 3D magnetic topology for two key concepts, the connection length L c and the effective magnetic mirror ratio M eff , and present a comprehensive picture of electron and ion dynamics related to the magnetic topology. The connection length determines the 3D structure of the ambipolar potential, and a sharp potential drop across distinct L c regions induces the E × B transport and mixing across the field line. The confinement of electrons and ions along the field line is determined by the ambipolar potential and [Formula: see text] configuration. Electron and ion temperatures in magnetic hills (M eff < 1) are lower than in magnetic wells (M eff > 1) because particles in magnetic hills are more likely to escape toward the wall boundary along the field line. The mixing between the magnetic wells and hills by E × B and magnetic drift motions results in collisionless detrapping of electrons and ions, which reduces their temperature efficiently. Numerical simulations of two different magnetic configurations demonstrate the importance of the collisionless detrapping mechanism, which could be the main cause of plasma temperature drop during the thermal quench.

70 PLASMA PHYSICS AND FUSION TECHNOLOGY↗

Flash Drought Onset and Development Mechanisms Captured With Soil Moisture and Vegetation Data Assimilation

Flash droughts evolve and intensify rapidly under the influence of anomalous atmospheric conditions. In this study, we investigate the role of assimilating remotely sensed soil moisture (SM) and vegetation properties in capturing the evolution and impacts of two flash droughts in the Northern Great Plains. We find that during 2016 drought triggered by anomalously high temperatures and excessive evaporative demands, multivariate data assimilation (DA) of MODIS-derived leaf area index (LAI) and Soil Moisture Active Passive SM within Noah-Multiparameterization model helps capture elevated transpiration at onset. Assimilation of LAI particularly helped model the resulting rapid decline in SM during onset with as high as 10.0% steeper rate of decline compared to the simulation without any assimilation. Modeled-SM anomalies exhibit a 7.5% and 11.7% increase in similarity with Evaporative Stress Index (ESI) data and U.S. Drought Monitor (USDM) maps, respectively. In contrast, during 2017 flash drought driven by record-low precipitation during summers, SM assimilation resulted in largest rates of decline in rootzone SM, as large as 48.4% compared to results from no assimilation. Multivariate DA of SM and LAI results in 6.7% and 14.3% higher spatial similarity with ESI and USDM, respectively, and is necessary to model rapid intensification caused by anomalous precipitation deficits. This study elucidates the need to incorporate multiple observational constraints from remote sensing to effectively capture rapid onset rates, intensification, and severity of flash drought following different propagation mechanisms. This is fundamental for drought early detection to provide a wider window of response and implement efficient mitigation strategies.

Shahryar K. Ahmad↗

Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet

Abstract BACKGROUND Peanut allergy is recognized as a major food allergy that triggers severe and even fatal symptoms. Avoidance of peanuts in the diet is the main option for current safety management. Processing techniques reducing peanut allergenicity are required to develop other options. Cold plasma is currently considered as a novel non‐thermal approach to alter protein structure and has the potential to alleviate immunoreactivity of protein allergen. RESULTS The application of a cold argon plasma jet to peanut protein extract could reduce the amount of a 64 kDa protein band corresponding to a major peanut allergen Ara h 1 using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, but the overall protein size distribution did not change significantly. A decrease in peanut protein solubility was a possible cause that led to the loss of protein content in the soluble fraction. Immunoblotting and enzyme‐linked immunosorbent assay elucidated that the immunoreactivity of Ara h 1 was significantly decreased with the time treated with plasma. Ara h 1 antigenicity reduced by 38% after five scans (approximately 3 min) of cold argon plasma jet treatment, and the reduction was up to 66% after approximately 15 min of treatment. CONCLUSION The results indicate that cold argon plasma jet treatment could be a suitable platform for alleviating the immunoreactivity of peanut protein. This work demonstrates an efficient, compact, and rapid platform for mitigating the allergenicity of peanuts, and shows great potential for the plasma platform as a non‐thermal technique in the food industry. © 2023 Society of Chemical Industry.

Hsu, Fu‐Chiun↗

Wasserstein Normalized Autoencoder for Anomaly Detection in ProtoDUNE Vertical-Drift Detector

ProtoDUNE Vertical Drift needs a selective triggering algorithm. The detector sits on Earth's surface, so cosmic activity dominates its data. Our goal in this paper is to trigger on neutrino events more robustly than the current deployed Analog-to-Digital Converter Simple Window (ADCSW) model and, eventually, search for signals of Beyond Standard Model (BSM) physics at DUNE as our ultimate North Star objective. As a step towards this goal, we evaluate a Wasserstein Normalized Autoencoder (WNAE) on simulated collection-plane only windows of shape $1\times10\times10$ where Neutrinos act as our BSM-proxy and Cosmic-ray Muons serve as our learned background. The network parameters are fitted using only cosmic-ray muon events as background in order to maintain an unsupervised pipeline. Training uses finite-step Langevin $x^-$ samples, positive-sample reconstruction energy, and an empirical sliced $2$-Wasserstein objective to learn a normalized Boltzmann energy model. We then calibrate on a nominal $5\,\mathrm{Hz}$ operating threshold calculated from cosmic validation data. Both WNAE and ADCSW accept 311 of 194,083 held-out cosmic background events at this $5\,\mathrm{Hz}$ threshold. We found that WNAE accepts 9,677 of 34,634 neutrino-proxy events $(27.9\pm0.24)\%$, compared with 10,076 $(29.1\pm0.24)\%$ for ADCSW, an observed WNAE-minus-ADCSW difference of $-1.15\%$. At another nominal $2\,\mathrm{Hz}$ target threshold, the corresponding efficiencies are $(20.5\pm0.22)\%$ and $(22.6\pm0.22)\%$, respectively. Of the WNAE-selected neutrino proxies at $5\,\mathrm{Hz}$, $(20.8\pm0.4)\%$ of the classified neutrino-proxy events are unique to WNAE, where the uncertainty is an absolute binomial standard error of $0.4\%$.

Zheng, Jake [U. Chicago (main)] (ORCID:00090002189↗

Wasserstein Normalized Autoencoder for Anomaly Detection in ProtoDUNE Vertical-Drift Detector

ProtoDUNE Vertical Drift needs a selective triggering algorithm. The detector sits on Earth's surface, so cosmic activity dominates its data. Our goal in this paper is to trigger on neutrino events more robustly than the current deployed Analog-to-Digital Converter Simple Window (ADCSW) model and, eventually, search for signals of Beyond Standard Model (BSM) physics at DUNE as our ultimate North Star objective. As a step towards this goal, we evaluate a Wasserstein Normalized Autoencoder (WNAE) on simulated collection-plane only windows of shape $1\times10\times10$ where Neutrinos act as our BSM-proxy and Cosmic-ray Muons serve as our learned background. The network parameters are fitted using only cosmic-ray muon events as background in order to maintain an unsupervised pipeline. Training uses finite-step Langevin $x^-$ samples, positive-sample reconstruction energy, and an empirical sliced $2$-Wasserstein objective to learn a normalized Boltzmann energy model. We then calibrate on a nominal $5\,\mathrm{Hz}$ operating threshold calculated from cosmic validation data. Both WNAE and ADCSW accept 311 of 194,083 held-out cosmic background events at this $5\,\mathrm{Hz}$ threshold. We found that WNAE accepts 9,677 of 34,634 neutrino-proxy events $(27.9\pm0.24)\%$, compared with 10,076 $(29.1\pm0.24)\%$ for ADCSW, an observed WNAE-minus-ADCSW difference of $-1.15\%$. At another nominal $2\,\mathrm{Hz}$ target threshold, the corresponding efficiencies are $(20.5\pm0.22)\%$ and $(22.6\pm0.22)\%$, respectively. Of the WNAE-selected neutrino proxies at $5\,\mathrm{Hz}$, $(20.8\pm0.4)\%$ of the classified neutrino-proxy events are unique to WNAE, where the uncertainty is an absolute binomial standard error of $0.4\%$.

Zheng, Jake [Chicago U.] (ORCID:0009000218901379)↗

An experimental study of ovarian cancer imaging and therapy by paclitaxel-loaded phase-transformation lipid nanoparticles combined with low-intensity focused ultrasound

Highlights: • PTX-anti-LHRHR-PTNPs is expected to become a new type of ultrasound contrast agent. • LIFU can enhance the efficiency of PTX-anti-LHRHR-PTNPs. • For ovarian cancer, PTX-anti-LHRHR-PTNPs has a good therapeutic effect. Drug-loaded phase-transformation lipid nanoparticles (NPs) combined with low-intensity focused ultrasound (LIFU) for ultrasound molecular imaging and therapy, which is a very promising drug carrier and can provide both physical and chemical therapeutics, simultaneously. We successfully prepared the paclitaxel (PTX) loaded anti-LHRHR targeted phase-transformation lipid nanoparticles (PTX-anti-LHRHR-PTNPs) for ovarian cancer in this study combined with LIFU has the following characteristics: On the one hand, it showed smaller size and greater stability than blood cells, which significantly prolonged its half-life in the body, and can actively target ovarian cancer OVCAR-3 cells, and smoothly penetrate the endothelial gap into the tumor site for specifically killing the ovarian cancer cells. Thereby, the special drug carrier improved the therapeutic effect and reduced toxic and side effects, maximized the protection of normal tissues and minimized adverse reactions. On the other hand, PTX-anti-LHRHR-PTNPs can be targeted to focus after being injected intravenously and remain in the tumor target tissue for a long time. At the same time, liquid-gas phase-transformation can occur under LIFU triggering, resulting in more ideal and sustained ultrasound imaging effects. Then acoustic contrast agent is used to develop the molecular level of ultrasound scattering, so as to evaluate the diseased tissue from the molecular level.

60 APPLIED LIFE SCIENCES↗

Experimental measurement of energy release from an initiating layer in an insensitive explosive

When subjected to a shock of insufficient strength to trigger prompt reaction, heterogenous condensed phase explosives can form regions where significant amounts of the explosive remain unreacted for times much greater than the reaction time of the detonating explosive. This phenomena is observed for the explosive PBX 9502 (95 wt% TATB) both for planar and oblique input shocks. Here, we build on previous results by performing cylinder expansion (CYLEX) tests where the explosive charge is comprised of a faster core of PBX 9501 (95 wt% HMX) inside a slower annulus of PBX 9502. The detonation in the faster PBX 9501 drives an oblique shock into the adjacent PBX 9502, and an annular transverse initiating layer (IL) results. In the test geometry, the IL travels steadily down the length of the test after a short run distance. At radial positions beyond the IL, an annular region of detonating PBX 9502 is observed. Using standard CYLEX test diagnostics, we infer the total energy release of this experiment. By making the assumptions that (1) the combined energy release is comprised of contributions from detonating PBX 9501, detonating PBX 9502, and the IL in the PBX 9502 and (2) mass-specific energy release for the detonating explosives is approximately the same as typically observed for each explosive, the IL energy release and reaction efficiency can be computed. Results are compared to prior results for a similar geometry, and indicate that while shock deadened PBX 9502 does not detonate promptly, it does eventually release a significant portion of its chemical potential energy over longer timescales on the order of 10 µs.

71 CLASSICAL AND QUANTUM MECHANICS, GENERAL PHYSIC↗

Dynamical Instabilities in Systems of Multiple Short-period Planets Are Likely Driven by Secular Chaos: A Case Study of Kepler-102

We investigated the dynamical stability of high-multiplicity Kepler and K2 planetary systems. Our numerical simulations find instabilities in ∼20% of the cases on a wide range of timescales (up to 5 × 10{sup 9} orbits) and over an unexpectedly wide range of initial dynamical spacings. To identify the triggers of long-term instability in multiplanet systems, we investigated in detail the five-planet Kepler-102 system. Despite having several near-resonant period ratios, we find that mean-motion resonances are unlikely to directly cause instability for plausible planet masses in this system. Instead, we find strong evidence that slow inward transfer of angular momentum deficit (AMD) via secular chaos excites the eccentricity of the innermost planet, Kepler-102 b, eventually leading to planet–planet collisions in ∼80% of Kepler-102 simulations. Kepler-102 b likely needs a mass ≳0.1 M {sub ⊕}, hence a bulk density exceeding about half Earth’s, in order to avoid dynamical instability. To investigate the role of secular chaos in our wider set of simulations, we characterize each planetary system’s AMD evolution with a “spectral fraction” calculated from the power spectrum of short integrations (∼5 × 10{sup 6} orbits). We find that small spectral fractions (≲0.01) are strongly associated with dynamical stability on long timescales (5 × 10{sup 9} orbits) and that the median time to instability decreases with increasing spectral fraction. Our results support the hypothesis that secular chaos is the driver of instabilities in many nonresonant multiplanet systems and also demonstrate that the spectral analysis method is an efficient numerical tool to diagnose long-term (in)stability of multiplanet systems from short simulations.

79 ASTRONOMY AND ASTROPHYSICS↗

How Many Kilonovae Can Be Found in Past, Present, and Future Survey Data Sets?

The discovery of a kilonova (KN) associated with the Advanced LIGO (aLIGO)/Virgo event GW170817 opens up new avenues of multi-messenger astrophysics. Here, using realistic simulations, we provide estimates of the number of KNe that could be found in data from past, present, and future surveys without a gravitational-wave trigger. For the simulation, we construct a spectral time-series model based on the DES-GW multi-band light curve from the single known KN event, and we use an average of BNS rates from past studies of ${10}^{3}\,{\mathrm{Gpc}}^{-3}\,{\mathrm{yr}}^{-1}$, consistent with the one event found so far. Examining past and current data sets from transient surveys, the number of KNe we expect to find for ASAS-SN, SDSS, PS1, SNLS, DES, and SMT is between 0 and 0.3. We predict the number of detections per future survey to be 8.3 from ATLAS, 10.6 from ZTF, 5.5/69 from LSST (the Deep Drilling/Wide Fast Deep), and 16.0 from WFIRST. The maximum redshift of KNe discovered for each survey is $z=0.8$ for WFIRST, $z=0.25$ for LSST, and $z=0.04$ for ZTF and ATLAS. This maximum redshift for WFIRST is well beyond the sensitivity of aLIGO and some future GW missions. For the LSST survey, we also provide contamination estimates from Type Ia and core-collapse supernovae: after light curve and template-matching requirements, we estimate a background of just two events. Finally, more broadly, we stress that future transient surveys should consider how to optimize their search strategies to improve their detection efficiency and to consider similar analyses for GW follow-up programs.

46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND ↗

Hierarchical Testing of a Hybrid Machine Learning‐Physics Global Atmosphere Model

Machine learning (ML)-based models have demonstrated high skill and computational efficiency, often outperforming conventional physics-based models in weather and subseasonal predictions. While prior studies have assessed their fidelity in capturing synoptic-scale atmospheric dynamics, their performance across timescales and under out-of-distribution forcing, such as +3K or +4K uniform-warming forcings, and the sources of biases remain elusive, to establish the model's reliability for Earth science. Here, we design three sets of experiments targeting synoptic-scale phenomena, interannual variability, and out-of-distribution uniform-warming forcings. We evaluate the Neural General Circulation Model (NeuralGCM), a hybrid model integrating a dynamical core with ML-based component, against observations and physics-based Earth system models (ESMs). At the synoptic scale, NeuralGCM captures the evolution and propagation of extratropical cyclones with performance comparable to ESMs. At the interannual scale, when forced by El Niño-Southern Oscillation sea surface temperature (SST) anomalies, NeuralGCM successfully reproduces associated teleconnection patterns but exhibits deficiencies in capturing nonlinear response. Under out-of-distribution uniform-warming forcings, NeuralGCM simulates similar responses in global-average temperature and precipitation and reproduces large-scale tropospheric circulation features similar to those in ESMs. Notable weaknesses include overestimating the tracks and spatial extent of extratropical cyclones, biases in the teleconnected wave train triggered by tropical SST anomalies, and differences in upper-level warming and stratospheric circulation responses to SST warming compared to physics-based ESMs. The causes of these weaknesses were explored. Despite the noted weaknesses, NeuralGCM reproduces responses across experiments reasonably and performs comparably to ESMs. By integrating a dynamical core with ML, NeuralGCM shows potential for developing ML-based ESMs.

global warming↗

Toward a Climate OSSE Framework for Satellite Mission Design

The rich history of observing system simulation experiments (OSSEs) does not yet include a well-established framework for using climate models. The need for a climate OSSE is triggered by the need to quantify the value of a particular measurement for reducing the uncertainty in climate predictions, which differ from numerical weather predictions in that they depend on future atmospheric composition rather than the current state of the weather. However, both weather and climate modeling communities share a need for motivating major observing system investments. Here, we outline a new framework for climate OSSEs that leverages the use of machine learning to calibrate climate model physics against existing satellite data. We demonstrate its application using NASA’s GISS-E3 model to objectively quantify the value of potential future improvements in spaceborne measurements of Earth’s planetary boundary layer. A mature climate OSSE framework should be able to quantitatively compare the ability of proposed observing system architectures to answer a climate-related question, thus offering added value throughout the mission design process, which is subject to increasingly rapid advances in instrument and satellite technology. Technical considerations include selection of observational benchmarks and climate projection metrics, approaches to pinpoint the sources of model physics uncertainty that dominate uncertainty in projections, and the use of instrument simulators. Community and policy-making considerations include the potential to interface with an established culture of model intercomparison projects and a growing need to economically assess the value-driven efficiency of social spending on Earth observations.

54 ENVIRONMENTAL SCIENCES↗

Exploring the Longwave Radiative Effects of Dust Aerosols

Dust aerosols not only affect air quality and visibility where they pose a significant health and safety risk, but they can also play a role in modulating the energy balance of the Earth-atmosphere system by directly interacting with local radiative fields. Consequently, dust aerosols can impact regional climate patterns such as changes in precipitation and the evolution of the hydrological cycle. Assessing the direct effect of dust aerosols at the solar wavelengths is fairly straightforward due in part to the relatively large signal-to-noise ratio in broadband irradiance measurements. The longwave (LW) impacts, on the other hand, are rather difficult to ascertain since the measured dust signal level (10 Wm-2) is on the same order as the instrumental uncertainties. Moreover, compared to the shortwave (SW), limited experimental data on the LW optical properties of dust makes it a difficult challenge for constraining the LW impacts. Owing to the strong absorption features found in many terrestrial minerals (e.g., silicates and clays), the LW effects, although much smaller in magnitude compared to the SW, can still have a sizeable impact on the energetics of the Earth-atmosphere system, which can potentially trigger changes in the heat and moisture surface budgets, and dynamics of the atmosphere. The current endeavor is an integral part of an on-going research study to perform detailed assessments of dust direct aerosol radiative effects (DARE) using comprehensive global datasets from NASA Goddards mobile ground-based facility (cf. http://smartlabs.gsfc.nasa.gov/) during previous field experiments near key dust source regions. Here we examine and compare the results from two of these studies: the 2006 NASA African Monsoon Multidisciplinary Activities and the 2008 Asian Monsoon Years. The former study focused on transported Saharan dust at Sal Island (16.73N, 22.93W), Cape Verde along the west coast of Africa while the latter focused on Asian dust at Zhangye China (39.082N, 100.276E), a semi-arid region between the Taklimakan and Gobi deserts. NASA Goddards Giovanni system is used to help map out the spatial distribution of retrieved aerosol optical depths across the latter desert regions. 1-D radiative transfer model constrained by local measurements, including spectral photometry/interferometry and lidar for characterizing the spatiotemporal variability in dust properties and atmospheric conditions, is employed to evaluate the local instantaneous LW DARE of dust both at the surface and at the top of the atmosphere along with heating rate profiles for cloud-free atmospheres. The efficiency in LW DARE and its significance relative to the diurnally averaged SW effects are explored and compared in both studies. Found to be non-negligible, LW DARE is an important component in the study of regional climate variation with important implications for more detailed global assessments.

Hansell, Richard A., Jr.↗

Irrigation Requirement Estimation using MODIS Vegetation Indices and Inverse Biophysical Modeling; A Case Study for Oran, Algeria

Human demand for food influences the water cycle through diversion and extraction of fresh water needed to support agriculture. Future population growth and economic development alone will substantially increase water demand and much of it for agricultural uses. For many semi-arid lands, socio-economic shifts are likely to exacerbate changes in climate as a driver of future water supply and demand. For these areas in particular, where the balance between water supply and demand is fragile, variations in regional climate can have potentially predictable effect on agricultural production. Satellite data and biophysically-based models provide a powerful method to quantify the interactions between local climate, plant growth and water resource requirements. In irrigated agricultural lands, satellite observations indicate high vegetation density while the precipitation amount indicates otherwise. This inconsistency between the observed precipitation and the observed canopy leaf density triggers the possibility that the observed high leaf density is due to an alternate source of water, irrigation. We explore an inverse process approach using observations from the Moderate Resolution Imaging Spectroradiometer (MODIS), climatological data, and the NASA's Simple Biosphere model, SiB2, to quantitatively assess water demand in a semi-arid agricultural land by constraining the carbon and water cycles modeled under both equilibrium (balance between vegetation and prevailing local climate) and nonequilibrium (water added through irrigation) conditions. We postulate that the degree to which irrigated lands vary from equilibrium conditions is related to the amount of irrigation water used. We added water using two distribution methods: The first method adds water on top of the canopy and is a proxy for the traditional spray irrigation. The second method allows water to be applied directly into the soil layer and serves as proxy for drip irrigation. Our approach indicates that over the study site, for the month of July, spray irrigation resulted in an irrigation amount of about 1.4 mm per occurrence with an average frequency of occurrence of 24.6 hours. The simulated total monthly irrigation for July was 34.85 mm. In contrast, the drip irrigation resulted in less frequent irrigation events with an average water requirement about 57% less than that simulated during the spray irrigation case. The efficiency of the drip irrigation method rests on its reduction of the canopy interception loss compared to the spray irrigation method. When compared to a country-wide average estimate of irrigation water use, our numbers are quite low. We would have to revise the reported country level estimates downward to 17% or less

Bounoua, L.↗

Impacts of Pasture Conversion to Sugarcane on Water Fluxes and Water Use Efficiency in the Southeastern US

The expansion of sugarcane (cane), a high-yielding perennial crop, will likely reshape the bioenergy landscape in the Southeastern US. However, its ecohydrological implications, particularly following conversion from grazed pastures, a dominant land use in the region, remain highly uncertain. We investigated the impact of cane expansion on evapotranspiration (ET) and its partitioning, and the mechanisms influencing both ET components and water use efficiency (WUE) across multiple scales and growth cycles in subtropical Florida. We combined eddy covariance, biometric measurements, and process-based stomatal conductance (g s ) models. ET was 1.7% lower in cane than in improved pasture (IMP) but exceeded that in semi-native pasture (SN) by 21%. Transpiration (T) followed a similar pattern, consistent with lower g s in cane relative to IMP. Cane had more conservative water use and greater sensitivity of g s to vapor pressure deficit (VPD) compared to IMP pasture, suggesting cane may be more tolerant of increasing atmospheric water demand. In contrast, SN showed lower g s and weaker stomatal sensitivity to VPD compared to cane, resulting in lower T. In cane, stomatal regulation and T varied across growth cycles, with stomata becoming less water conservative as stands matured, highlighting the importance of incorporating stand age-dependent stomatal regulation into hydrological models. Evaporation (E) was higher in cane than pastures (19%–26%), partially offsetting WUE gains. Cane exhibited higher intrinsic WUE (GPP/g s ; Gross Primary Productivity), ecosystem WUE (GPP/ET), and harvest WUE (harvest/ET) than both pasture types. Large-scale pasture-to-cane conversion could produce widely contrasting hydrological outcomes. The net regional impact will depend on the proportion of each pasture type converted and on cane's high g s sensitivity to VPD, which triggers tight stomatal regulation and conservative water use, both of which will become increasingly consequential under intensifying atmospheric water demand.

bioenergy↗

Trace Gas Measurements on Mars and Earth Using Optical Parametric Generation

Trace gases and their isotopic ratios in planetary atmospheres offer important but subtle clues as to the origins of a planet's atmosphere, hydrology, geology, and potential for biology. An orbiting laser remote sensing instrument is capable of measuring trace gases on a global scale with unprecedented accuracy, and higher spatial resolution that can be obtained by passive instruments. We have developed an active sensing instrument for the remote measurement of trace gases in planetary atmospheres (including Earth). The technique uses widely tunable, seeded optical parametric generation (OPG) to measure methane, CO2, water vapor, and other trace gases in the near and mid-infrared spectral regions. Methane is a strong greenhouse gas on Earth and it is also a potential biogenic marker on Mars and other planets. Methane in the Earth's atmosphere survives for a shorter time than CO2 but its impact on climate change can be larger than CO2. Methane levels have remained relatively constant over the last decade around 1.78 parts per million (ppm) but recent observations indicate that methane levels may be on the rise. Increasing methane concentrations may trigger a positive feedback loop and a subsequent runaway greenhouse effect, where increasing temperatures result in increasing methane levels. The NRC Decadal Survey recognized the importance of global observations of greenhouse gases and called for simultaneous CH4, CO, and CO2 measurements but also underlined the technological limitations for these observations. For Mars, methane measurements are of great interest because of its potential as a strong biogenic marker. A remote sensing instrument that can measure day and night over all seasons and latitudes can identify and localize sources of biogenic gas plumes produced by subsurface chemistry or biology, and aid in the search for extra-terrestrial life. It can identify the dynamics of methane generation over time and latitude and identify future lander mission sites for more detailed in-situ analysis. In this paper we report on remote sensing measurements of methane using a high peak power, widely tunable optical parametric generator (OPG) operating at 3.3 micron and 1.65 micron. The OPG is pumped by a passively q-switched single frequency laser (3ns, 5KHz, 50uJ) and seeded by a diode laser. The spectral width of both signal and idler of seeded OPG is nearly Fourier transform limited. The output of seeded OPG is single frequency with high spectral purity and is widely tunable. Both 1650 nm and 3300 nm can be generated with a conversion efficiency of more than 30%. We have demonstrated detection of methane at 3274 nm and 1650 nm in a cell and also performed open path atmospheric measurements of methane at the same wavelengths. Finally, we were able to demonstrate simultaneous detection of methane at 3270.4 nm and CO2 at 1578.2 nm. In this paper we will discuss the OPG performance and atmospheric open path measurement results.

Numata, Kenji↗