Conformational alterations in unidirectional ion transport of a light-driven chloride pump revealed using X-ray free electron lasers
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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.
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Please check the README file for more information about the dataset.
The mask file and the geometry files are in the mask_and_geometry folder. Structure factor files are in the mtz folder. Stream files atr in the stream_files folder. Associated PDBIDs are 8C1U, 8C6F,
XFEL diffraction frames from batch-crystallized Soybean Lipoxygenase-1 (SLO-1), revealing two crystal polymorphs arising from differential hydration during sample delivery
ASHRAE Guideline 36 provides guidance for how typical commercial building HVAC systems should be controlled to reduce energy costs and improve the indoor environmental quality for occupants. An industry-led effort is developing test scripts for how control manufacturers can test that the control logic in their control products follows Guideline 36. This software intends to support that effort in two ways. First, by providing a testbed for control manufacturers to run the test scripts against their own control products and compare their generated results to the expected results provided by the test scripts. Second, by providing a testbed that can be used during the development of test scripts to rapidly and repeatably verify they exercise Guideline 36 logic as desired and generate accurate expected results that control products can be compared to. An initial version of the software has already been developed and released open-source by a previous CEC-funded project that implemented the first use case (https://github.com/LBNL-ETA/guideline36_conformance_test/blob/master/LICENSE.txt), while this software disclosure will add the functionality of the second use case, as well as update the software where needed, all open-source. We intend to extend and contribute to the existing open-source software repository.
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Abstract not provided
Summary Gel treatment is an effective way to attack excessive water production during oil development. The transport behavior of gel materials in reservoirs is of crucial importance to the effectiveness of gel treatments. The aim of this paper is investigating the transport behavior of swellable micrometer-sized preformed particle gels (PPGs, or microgels) through superpermeable (super-K) channels. Sandpacks with permeabilities ranging from 27 to 221 darcies were used to mimic the super-K channels. Multiple pressure sensors were applied along the sandpack models to monitor the propagation behavior of the microgels. The tested microgel particles could transport through the super-K channels, and a higher driving pressure gradient was required when the particle/pore size ratio was larger. The pressure gradient distribution along the super-K channels was relatively uniform when the particle/pore ratio was low (less than 1.3). However, the inlet section would show increasingly higher pressure gradients as the particle/pore ratio was increased, indicating increased difficulty in propagation. The propagation of the gel particles was significantly slower compared with the carrying fluid. The delayed propagation behavior was more pronounced when the particle/pore ratio was higher. The injection pressure was much less sensitive to the injection flow rate compared with a Newtonian fluid. The gel dispersion exhibited an apparent shear thinning (pseudoplastic) behavior when transporting through the porous channels. Breakage of the gel particles was observed especially at high superficial velocities. The particle breakage was partially responsible for the apparent shear thinning behavior. The breakage phenomenon was in favor of deep placement of the gel particles. The channel permeabilities were significantly reduced by the microgels, bringing sufficient resistance to subsequent waterflooding (more than 99.5%). At given matching size conditions, softer gels were more likely to establish in-depth placement and uniform water blocking capacity in the channels. The microgel particles exhibited salinity-responsive behavior to the post-brine flush. The gel particles could shrink and reswell according to the salinity of the injected water. Possibilities were discussed to use this salinity-responsive behavior. Also, the microgels exhibited a particular disproportionate permeability reduction (DPR) effect. After gel injection, the channel permeability to water flow was reduced by more than 20 to 92 times of the permeability to oil flow. This work provides important support to understand the transport behavior of gel particles in super-K channels. The achievements are helpful for gel product selection and gel treatment design.
The goal of this research project was to further our understanding of biological electron transfer through the development and application of new methods for studying proteins and their complexes with high spatial and temporal detail. The approach was based on incorporation of reporter bonds with distinct vibrational frequencies at specific locations in proteins and their characterization through the interaction of the bonds with pulses of infrared light. The project focused on the proteins plastocyanin (Pc) and cytochrome (cyt) f, two electron transfer partners in photosynthesis. The first thrust of the project was the incorporation of carbon deuterium bonds as non-perturbative probes at a ligand to the copper ion of Pc and their use to detect changes in the bonding due to the number of electrons at the metal and how the bonding changes upon association with cyt f. Toward developing more advanced approaches to characterize proteins, we incorporated a stronger reporter, the cyano group of cyanophenylalanine, at several locations in Pc and measured the frequency dynamics in the absence and presence of cyt f. The acquired data improved the current model for the protein complex. Notably, the data indicated that the majority of the population is involved in a loosely bound complex, providing insight into the nature of the ensembles formed by electron transfer protein complexes that is not available via other approaches. In addition to learning about the protein complex, we made strides toward developing and improving the new experimental approach to study highly dynamic behaviors of proteins.
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Various graphs are included for Progress Report 1 for the project.
We set ambitious goals to examine microorganism communities and measure both their chemical input and output as a read out of specific biochemical activity. These scientific goals are driving the development of sophisticated algorithms to analyze large amounts of experimental measurements made using high throughput technologies to explain and predict how the environment influences biological function at multiple scales and how the microbial systems in tum modify the environment. By examining how bacteria communities rely on symbiotic metabolic relationships for survival and reproductive success, and how these relationships consequently affect their biochemical capabilities. This was accomplished using state-of-the-art mass spectrometry-based methods, and metabolic fingerprinting approaches with a high degree of chemical specificity and sensitivity. During this period the original effort transitioned and was consolidated into what is now ENIGMA, the efforts on technology development expanded to include more untargeted metabolomics and its application to organisms on a systems level.
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Abstract not provided.
MQXFA07 did not meet acceptance requirement during vertical test. This report presents the analysis of MQXFA07 components related to the limited test performance, and the damage mechanism as it was understood by the MQXFA team.
This project had three major goals: Explore qheories of composite dark matter and composite Higgs bosons through the work of the Lattice Strong Dynamics (LSD) collaboration.; Extend lattice gauge theories to curved manifolds through the work of the Quantum Finite Elements (QFE) collaboration.; Explore normalizing flow neural networks for producing ensembles of lattice gauge theory configurations.
The scientific and technological exploration of artificially designed three-dimensional magnetic nanostructures opens the path to exciting novel physical phenomena, originating from the increased complexity in spin textures, topology, and frustration in three dimensions. Theory predicts that the equilibrium magnetic ground state of two-dimensional systems which reflects the competition between symmetric (Heisenberg) and antisymmetric (Dzyaloshinskii-Moriya interaction (DMI)) exchange interaction is significantly modified on curved surfaces when the radius of local curvature becomes comparable to fundamental magnetic length scales. In this CRADA we performed collaboratively an experimental study of the spin texture in an 8 nm thin magnetic multilayer with growth-induced in-plane anisotropy and DMI deposited onto the curved surface of a 1.8µm long non-magnetic carbon nanowire with a 67 nm radius. The sample was fabricated in Juelich and with collaborators at BESSY in Berlin. Using magnetic soft x-ray tomography at the ALBA synchrotron in Spain, the three-dimensional spin configuration in this nanotube was retrieved with about 30nm spatial resolution. The transition between two vortex configurations on the two ends of the nanotube with opposite circulation occurs through a domain wall that is aligned at an inclined angle relative to the wire axis. Three-dimensional micromagnetic simulations support the experimental observations and represent a visualization of the curvature-mediated DMI. They also allow a quantitative estimate of the DMI value for the magnetic multilayered nanotube.
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