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At least 595 records · Page 33

Polyamine binding to proteins in oat and Petunia protoplasts

Previous work (A Apelbaum et al. [1988] Plant Physiol 88: 996-998) has demonstrated binding of labeled spermidine (Spd) to a developmentally regulated 18 kilodalton protein in tobacco tissue cultures derived from thin surface layer explants. To assess the general importance of such Spd-protein complexes, we attempted bulk isolation from protoplasts of Petunia and oat (Avena sativa). In Petunia, as in tobacco, fed radioactive Spd is bound to protein, but in oat, Spd is first converted to 1,3,-diaminopropane (DAP), probably by polyamine oxidase action. In oat, binding of DAP to protein depends on age of donor leaf and conditions of illumination and temperature, and the extraction of the DAP-protein complex depends upon buffer and pH. The yield of the DAP-protein complex was maximized by extraction of frozen-thawed protoplasts with a pH 8.8 carbonate buffer containing SDS. Its molecular size, based on Sephacryl column fractionation of ammonium sulfate precipitated material, exceeded 45 kilodaltons. Bound Spd or DAP can be released from their complexes by the action of Pronase, but not DNAse, RNAse, or strong salt solutions, indicating covalent attachment to protein.

NASA Discipline Plant Biology↗

Ditching Tests of a 1/9-Size Model of the Army P-38 Airplane in Langley Tank No. 2 and at the Outdoor Catapult

A dynamically similar model of the Army P-38 airplane was tested to determine the best way to land this airplane on the water and to determine its probable ditching performance. The tests consisted of ditching the model at various landing attitudes, flap settings, speeds, weights, and conditions of simulated damage. The model was ditched in calm water from the tank towing carriage and a few ditching were made in both calm and rough water at the outdoor catapult. The performance of the model was determined by making visual observations, by recording lengths of run and time histories of decelerations, and by taking motion pictures of the ditchings.

Jarvis, George A.↗

Evaluation of GEOS Total Cloud Fraction with GLOBE Citizen Science Observations and Co-Located Satellite Data

Here we evaluate the total cloud fraction in cycled forecast experiments with the NASA Global Earth Observing System (GEOS) model. Forecasts were run for summer and winter periods of 2017/2018 and compared with ground-based and satellite observations. Citizen science observations from the Global Learning and Observations to Benefit the Environment (GLOBE) Program were matched with MODIS satellite overpasses and geostationary cloud fraction, yielding a dataset of coincident observations for comparison with hourly model output. The observations indicate a model bias toward overcast and clear conditions, with underestimation of intermediate cloud fractions. We investigate underestimation of variance in the sub-grid total water probability density function (PDF) as a possible cause. The PDF determines large-scale cloud fraction as the sub-grid fraction with total water exceeding the saturation specific humidity. We conduct model experiments in which the shape, width, and spatial dependence of the PDF are varied, and consider the impacts on cloud fraction relative to the combined observations.

Starke, Matthew↗

Mars Exploration Rover Opportunity End of Mission Report

The Mars Exploration Rover (MER) Opportunity landed on Meridiani Planum on 25 January 2004 for a prime mission designed to last three months (90 sols). After more than fourteen years operating on the surface of Mars, the last communication from Opportunity occurred on sol 5111 (10 June, 2018) when a major dust storm reduced power on the solar panels to the point where further communications were not possible. Following the cessation of the dust storm several weeks later, the MER project radiated over 1000 commands to Mars in an attempt to elicit a response from the rover. Attempts were made utilizing the Deep Space Network X-Band and UHF relay via both Mars Odyssey and the Mars Reconnaissance Orbiter. Search and recovery efforts concluded on 12 February, 2019. It is the MER project’s assessment that the environmental window in which it would be most probable to recover Opportunity had passed by that time and that the rover would succumb to the extreme environmental conditions experienced during a winter on Mars. This report summarizes the major science accomplishments throughout the fourteen years of this mission, with a detailed focused on recent science accomplishments during the last extended mission (EM-11). This report also describes the mission engineering accomplishments and specific actions taken during the attempt to recover the vehicle after communications were lost during the major dust storm.

Callas, John L.↗

Brain wave correlates of attentional states: Event related potentials and quantitative EEG analysis during performance of cognitive and perceptual tasks

The increased use of automation in the cockpits of commercial planes has dramatically decreased the workload requirements of pilots, enabling them to function more efficiently and with a higher degree of safety. Unfortunately, advances in technology have led to an unexpected problem: the decreased demands on pilots have increased the probability of inducing 'hazardous states of awareness.' A hazardous state of awareness is defined as a decreased level of alertness or arousal which makes an individual less capable of reacting to unique or emergency types of situations. These states tend to be induced when an individual is not actively processing information. Under such conditions a person is likely to let his/her mind wander, either to internal states or to irrelevant external conditions. As a result, they are less capable of reacting quickly to emergency situations. Since emergencies are relatively rare, and since the high automated cockpit requires progressively decreasing levels of engagement, the probability of being seduced into a lowered state of awareness is increasing. This further decreases the readiness of the pilot to react to unique circumstances such as system failures. The HEM Lab at NASA-Langley Research Center has been studying how these states of awareness are induced and what the physiological correlates of these different states are. Specifically, they have been interested in studying electroencephalographic (EEG) measures of different states of alertness to determine if such states can be identified and, hopefully, avoided. The project worked on this summer involved analyzing the EEG and the event related potentials (ERP) data collected while subjects performed under two conditions. Each condition required subjects to perform a relatively boring vigilance task. The purpose of using these tasks was to induce a decreased state of awareness while still requiring the subject to process information. Each task involved identifying an infrequently presented target stimulus. In addition to the task requirements, irrelevant tones were presented in the background. Research has shown that even though these stimuli are not attended, ERP's to them can still be elicited. The amplitude of the ERP waves has been shown to change as a function of a person's level of alertness. ERP's were also collected and analyzed for the target stimuli for each task. Brain maps were produced based on the ERP voltages for the different stimuli. In addition to the ERP's, a quantitative EEG (QEEG) was performed on the data using a fast Fourier technique to produce a power spectral analysis of the EEG. This analysis was conducted on the continuous EEG while the subjects were performing the tasks. Finally, a QEEG was performed on periods during the task when subjects indicated that they were in an altered state of awareness. During the tasks, subjects were asked to indicate by pressing a button when they realized their level of task awareness had changed. EEG epochs were collected for times just before and just after subjects made this reponse. The purpose of this final analysis was to determine whether or not subjective indices of level of awareness could be correlated with different patterns of EEG.

Freeman, Frederick G.↗

Investigations of Air-Cooled Turbine Rotors for Turbojet Engines. 1: Experimental Disk Temperature Distribution in Modified J33 Split-Disk Rotor at Speeds up to 6000 RPM

An experimental investigation is being conducted at the Lewis laboratory to establish general principles for the design of noncritical turbine rotor configurations. This investigation includes evaluation of cooling effectiveness, structural stability, cooling-air flow distribution characteristics, and methods of supplying cooling air to the turbine rotor blades. Prior to design of a noncritical rotor, a standard turbine rotor of a commerical turbojet engine was split in the plane of rotation and machined to provide a passage for distributing cooling air to the base of each blade. The rotor was fitted with nontwisted, hollow, aircooled blades containing nine tubes in the coolant passage. In the investigation reported herein, the modified turbine rotor operated successfully up to speeds of 6000 rpm with ratios of cooling-air to combustion-gas flow as low as 0.02. The disk temperatures observed at these conditions were below 450 0 F when cooling air at 100 F was used from the laboratory air system. The calculated disk temperatures based on the correlation method presented for rated engine conditions were well below 1000 F at a cooling-air flow ratio of 0.02, which is considered adequate for a noncritical rotor. An appreciable difference in temperature level existed between the forward and rear disks. This temperature difference probably introduced undesirable disk stress distributions as a result of the relative elongations of the two disks. This investigation was terminated at 6000 rpm so that slight changes in the engine configuration could be made to relieve this condition.

Schramm, Wilson B.↗

The Integrated Medical Model: Statistical Forecasting of Risks to Crew Health and Mission Success

The Integrated Medical Model (IMM) helps capture and use organizational knowledge across the space medicine, training, operations, engineering, and research domains. The IMM uses this domain knowledge in the context of a mission and crew profile to forecast crew health and mission success risks. The IMM is most helpful in comparing the risk of two or more mission profiles, not as a tool for predicting absolute risk. The process of building the IMM adheres to Probability Risk Assessment (PRA) techniques described in NASA Procedural Requirement (NPR) 8705.5, and uses current evidence-based information to establish a defensible position for making decisions that help ensure crew health and mission success. The IMM quantitatively describes the following input parameters: 1) medical conditions and likelihood, 2) mission duration, 3) vehicle environment, 4) crew attributes (e.g. age, sex), 5) crew activities (e.g. EVA's, Lunar excursions), 6) diagnosis and treatment protocols (e.g. medical equipment, consumables pharmaceuticals), and 7) Crew Medical Officer (CMO) training effectiveness. It is worth reiterating that the IMM uses the data sets above as inputs. Many other risk management efforts stop at determining only likelihood. The IMM is unique in that it models not only likelihood, but risk mitigations, as well as subsequent clinical outcomes based on those mitigations. Once the mathematical relationships among the above parameters are established, the IMM uses a Monte Carlo simulation technique (a random sampling of the inputs as described by their statistical distribution) to determine the probable outcomes. Because the IMM is a stochastic model (i.e. the input parameters are represented by various statistical distributions depending on the data type), when the mission is simulated 10-50,000 times with a given set of medical capabilities (risk mitigations), a prediction of the most probable outcomes can be generated. For each mission, the IMM tracks which conditions occurred and decrements the pharmaceuticals and supplies required to diagnose and treat these medical conditions. If supplies are depleted, then the medical condition goes untreated, and crew and mission risk increase. The IMM currently models approximately 30 medical conditions. By the end of FY2008, the IMM will be modeling over 100 medical conditions, approximately 60 of which have been recorded to have occurred during short and long space missions.

Fitts, M. A.↗

Lunar and Asteroid Composition Using a Remote Secondary Ion Mass Spectrometer

Laboratory experiments simulating solar wind sputtering of lunar surface materials have shown that solar wind protons sputter secondary ions in sufficient numbers to be measured from low-altitude lunar orbit. Secondary ions of Na, Mg, Al, Si, K, Ca, Mn, Ti, and Fe have been observed sputtered from sample simulants of mare and highland soils. While solar wind ions are hundreds of times less efficient than those used in standard secondary ion mass spectrometry, secondary ion fluxes expected at the Moon under normal solar wind conditions range from approximately 10 to greater than 10(exp 4) ions cm(sup -2)s(sup -1), depending on species. These secondary ion fluxes depend both on concentration in the soil and on probability of ionization; yields of easily ionized elements such as K and Na are relatively much greater than those for the more electronegative elements and compounds. Once these ions leave the surface, they are subject to acceleration by local electric and magnetic fields. For typical solar wind conditions, secondary ions can be accelerated to an orbital observing location. The same is true for atmospheric atoms and molecules that are photoionized by solar EUV. The instrument to detect, identify, and map secondary ions sputtered from the lunar surface and photoions arising from the tenuous atmosphere is discussed.

Elphic, R. C.↗

Plant Adaptation to Cold. 1: Chlorophyll. 2: Minerals

A number of montane herbs in northern Utah typically form flower buds beneath the snow cover and flower either through it or immediately after its recession. Two of these species, one naturally occurring, Claytonia lanceolata, and one cultivated bulb, Galanthus nivalis, were investigated for their response to this stress environment. Snow depth patterns, chlorophyll content of tissues, and plants grown in light-tight boxes, suggest that light passing through the snow to reach plants growing underneath is not critically involved in the timing of their developmental cycles or in their ability to endure this low temperature environment. Ability to endure stress seems to be closely related in a number of ways to activity at the plant membranes. Plants were protected from low temperature damage by application of cytokinin or calcium, both of which probably acted at the membrane. Potassium calcium antagonisms were reflected in the internal distribution of the ions under natural stress conditions; and plants that differentiated at the meristem while growing through the snow accumulated calcium at the tip during this growth.

Rosen, P.↗

On signal design by the R/0/ criterion for non-white Gaussian noise channels

The use of the cut-off rate criterion for modulation system design is investigated for channels with non-white Gaussian noise. A signal space representation of the waveform channel is developed, and the cut-off rate for vector channels with additive non-white Gaussian noise and unquantized demodulation is derived. When the signal input to the channel is a continuous random vector, maximization of the cut-off rate with constrained average signal energy leads to a water-filling interpretation of optimal energy distribution in signal space. The necessary condition for a finite signal set to maximize the cut-off rate with constrained energy and an equally likely probability assignment of signal vectors is presented, and an algorithm is outlined for numerically computing the optimum signal set. As an example, the rectangular signal set which has the water-filling average energy distribution and the optimum rectangular set are compared.

Bordelon, D. L.↗

Atmospheric corrosion model and monitor for low cost solar arrays

An atmospheric corrosion model and corrosion monitoring system has been developed for low cost solar arrays (LSA). The corrosion model predicts that corrosion rate is the product of the surface condensation probability of water vapor and the diffusion controlled corrosion current. This corrosion model is verified by simultaneous monitoring of weather conditions and corrosion rates at the solar array test site at Mead, Nebraska.

Kaelble, D. H.↗

Galilean satellites - Evolutionary paths in deep resonance

While the Laplace resonance among the three inner Galilean satellites possesses stable configurations where the mean motions taken by pairs are in nearly 2:1 ratios, or 'deep resonance', the current satellite configuration is unstable near this exact commensurability. There is presently noted, however, a continuous path of stable conditions branching toward deep resonance which furnishes a track for the tidal evolution of the system and renders scenarios involving (probably episodic) evolution from deep resonance viable; this eliminates the requirement for rapid tidal dissipation of Jupiter by the alternative equilibrium hypothesis.

Greenberg, Richard↗

The General Necessary Condition for the Validity of Dirac's Transition Perturbation Theory

For the first time, from the natural requirements for the successive approximation the general necessary condition of validity of the Dirac's method is explicitly established. It is proved that the conception of 'the transition probability per unit time' is not valid. The 'super-platinium rules' for calculating the transition probability are derived for the arbitrarily strong time-independent perturbation case.

Quang, Nguyen Vinh↗

Acceptance Testing of a Satellite SCADA Photovoltaic-Diesel Hybrid System

Satellite Supervisory Control and Data Acquisition (SCADA) of a Photovoltaic (PV)/diesel hybrid system was tested using NASA's Advanced Communication Technology Satellite (ACTS) and Ultra Small Aperture Terminal (USAT) ground stations. The setup consisted of a custom-designed PV/diesel hybrid system, located at the Florida Solar Energy Center (FSEC), which was controlled and monitored at a "remote" hub via Ka-band satellite link connecting two 1/4 Watt USATs in a SCADA arrangement. The robustness of the communications link was tested for remote monitoring of the health and performance of a PV/diesel hybrid system, and for investigating load control and battery charging strategies to maximize battery capacity and lifetime, and minimize loss of critical load probability. Baseline hardware performance test results demonstrated that continuous two-second data transfers can be accomplished under clear sky conditions with an error rate of less than 1%. The delay introduced by the satellite (1/4 sec) was transparent to synchronization of satellite modem as well as to the PV/diesel-hybrid computer. End-to-end communications link recovery times were less than 36 seconds for loss of power and less than one second for loss of link. The system recovered by resuming operation without any manual intervention, which is important since the 4 dB margin is not sufficient to prevent loss of the satellite link during moderate to heavy rain. Hybrid operations during loss of communications link continued seamlessly but real-time monitoring was interrupted. For this sub-tropical region, the estimated amount of time that the signal fade will exceed the 4 dB margin is about 10%. These results suggest that data rates of 4800 bps and a link margin of 4 dB with a 1/4 Watt transmitter are sufficient for end-to-end operation in this SCADA application.

Kalu, A.↗

Transonic Unsteady Aerodynamics of the F/A-18E at Conditions Promoting Abrupt Wing Stall

A transonic wind tunnel test of an 8% F/A-18E model was conducted in the NASA Langley Research Center (LaRC) 16-Foot Transonic Tunnel (16-Ft TT) to investigate the Abrupt Wing Stall (AWS) characteristics of this aircraft. During this test, both steady and unsteady measurements of balance loads, wing surface pressures, wing root bending moments, and outer wing accelerations were performed. The test was conducted with a wide range of model configurations and test conditions in an attempt to reproduce behavior indicative of the AWS phenomenon experienced on full-scale aircraft during flight tests. This paper focuses on the analysis of the unsteady data acquired during this test. Though the test apparatus was designed to be effectively rigid. model motions due to sting and balance flexibility were observed during the testing, particularly when the model was operating in the AWS flight regime. Correlation between observed aerodynamic frequencies and model structural frequencies are analyzed and presented. Significant shock motion and separated flow is observed as the aircraft pitches through the AWS region. A shock tracking strategy has been formulated to observe this phenomenon. Using this technique, the range of shock motion is readily determined as the aircraft encounters AWS conditions. Spectral analysis of the shock motion shows the frequencies at which the shock oscillates in the AWS region, and probability density function analysis of the shock location shows the propensity of the shock to take on a bi-stable and even tri-stable character in the AWS flight regime.

Schuster, David M.↗

Hard and Soft Constraints in Reliability-Based Design Optimization

This paper proposes a framework for the analysis and design optimization of models subject to parametric uncertainty where design requirements in the form of inequality constraints are present. Emphasis is given to uncertainty models prescribed by norm bounded perturbations from a nominal parameter value and by sets of componentwise bounded uncertain variables. These models, which often arise in engineering problems, allow for a sharp mathematical manipulation. Constraints can be implemented in the hard sense, i.e., constraints must be satisfied for all parameter realizations in the uncertainty model, and in the soft sense, i.e., constraints can be violated by some realizations of the uncertain parameter. In regard to hard constraints, this methodology allows (i) to determine if a hard constraint can be satisfied for a given uncertainty model and constraint structure, (ii) to generate conclusive, formally verifiable reliability assessments that allow for unprejudiced comparisons of competing design alternatives and (iii) to identify the critical combination of uncertain parameters leading to constraint violations. In regard to soft constraints, the methodology allows the designer (i) to use probabilistic uncertainty models, (ii) to calculate upper bounds to the probability of constraint violation, and (iii) to efficiently estimate failure probabilities via a hybrid method. This method integrates the upper bounds, for which closed form expressions are derived, along with conditional sampling. In addition, an l(sub infinity) formulation for the efficient manipulation of hyper-rectangular sets is also proposed.

Crespo, L.uis G.↗

Component Performance Investigation of J71 Type II Turbines: III - Overall Performance of J71 Type IIA Turbine

The over-all component performance characteristics of the J71 Type IIA three-stage turbine were experimentally determined over a range of speed and over-all turbine total-pressure ratio at inlet-air conditions af 35 inches of mercury absolute and 700 deg. R. The results are compared with those obtained for the J71 Type IIF turbine, which was previously investigated, the two turbines being designed for the same engine application. Geometrically the two turbines were much alike, having the same variation of annular flow area and the same number of blades for corresponding stator and rotor rows. However, the blade throat areas downstream of the first stator of the IIA turbine were smaller than those of the IIF; and the IIA blade profiles were curve-backed, whereas those of the IIF were straight-backed. The IIA turbine passed the equivalent design weight flow and had a brake internal efficiency of 0.880 at design equivalent speed and work output. A maximum efficiency of 0.896 occurred at 130 percent of design equivalent speed and a pressure ratio of 4.0. The turbine had a wide range of efficient operation. The IIA turbine had slightly higher efficiencies than the IIF turbine at comparable operating conditions. The fact that the IIA turbine obtained the design equivalent weight flow at the design equivalent operating point was probably a result of the decrease in the blading throat areas downstream of the first stator from those of the IIF turbine, which passed 105 percent of design weight flow at the corresponding operating point. The third stator row of blades of the IIA turbine choked at the design equivalent speed and at an over-all pressure ratio of 4.2; the third rotor choked at a pressure ratio of approximately 4.9

Schum, Harold J.↗