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At least 577 records · Page 32

Closure Report for Corrective Action Unit 366: Area 11 Plutonium Valley Dispersion Sites, Nevada National Security Site, Nevada

This Closure Report (CR) presents information supporting closure of Corrective Action Unit (CAU) 366, Area 11 Plutonium Valley Dispersion Sites, and provides documentation supporting the completed corrective actions and confirmation that closure objectives for CAU 366 were met. This CR complies with the requirements of the Federal Facility Agreement and Consent Order (FFACO) that was agreed to by the State of Nevada; the U.S. Department of Energy (DOE), Environmental Management; the U.S. Department of Defense; and DOE, Legacy Management (FFACO, 1996 as amended).

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Nevada National Security Site Site-Directed Research and Development FY 2014 Annual Report

The reports contained herein are for project activities that occurred from October 2013 through September 2014. Project life cycle is indicated under the title as well as the original proposal number (in the following format: site abbreviation--ID #--originating fiscal year; e.g., STL-03-14). Each of the reports describes in detail the discoveries, achievements, and challenges encountered by our principal investigators. As SDRD, by definition, invests in “high-risk” and hopefully “high-payoff” research, the element of uncertainty is inherent. While many of our efforts are “successful” and result in positive outcomes or technology utilization, some fall short of expectations, but cannot be construed as “failure” in the negative sense. The latter is a natural and valid part of the process of advanced research and often leads to unforeseen new pathways to future discovery. Regardless, either result advances our knowledge base and increases our ability to identify solutions and/or avoid costly and unwarranted paths for future challenges. In summary, the SDRD program continues to provide an unfettered mechanism for innovation that returns multifold to our customers, to national security, and to the general public. The program is a vibrant R&D innovation engine, benefited by its discretionary pedigree, enhanced mission spectrum, committed resources, and sound competitiveness to yield maximum taxpayer benefit. The 25 projects described exemplify the creativity and ability of a diverse scientific and engineering talent base. The efforts also showcase an impressive capability and resource that can be brought to find solutions to a broad array of technology needs and applications relevant to the NNSS mission and national security. Further SDRD performance metrics can be found in the appendix at the end of this report.

36 MATERIALS SCIENCE↗

Radiological and Environmental Monitoring at the Clean Slate I and III Sites, Tonopah Test Range, Nevada, With Emphasis on the Implications for Off-site Transport

In 1963, the U.S. Department of Energy (DOE) (formerly the Atomic Energy Commission [AEC]) implemented Operation Roller Coaster on the Tonopah Test Range (TTR) and an adjacent area of the Nevada Test and Training Range (NTTR) (formerly the Nellis Air Force Range [NAFR]). Operation Roller Coaster consisted of four tests in which chemical explosions were detonated in the presence of nuclear devices to assess the dispersal of radionuclides and evaluate the effectiveness of storage structures to contain the ejected radionuclides. These tests resulted in the dispersal of plutonium over the ground surface downwind of the test ground zero (GZ). Three tests—Clean Slate I, II, and III—were conducted on the TTR in Cactus Flat. The fourth, Double Tracks, was conducted in Stonewall Flat on the NTTR. The Desert Research Institute (DRI) installed two monitoring stations in 2008, Station 400 at the Sandia National Laboratories (SNL) Range Operations Center (ROC) and Station 401 at Clean Slate III. Station 402 was installed at Clean Slate I in 2011 to measure radiological, meteorological, and dust conditions. The monitoring activity was implemented to determine if radionuclide contamination in the soil at the Clean Slate sites was being transported beyond the contamination area boundaries. Some of the data collected also permits comparison of radiological exposure at the TTR monitoring stations to conditions observed at Community Environmental Monitoring Program (CEMP) stations around the NTTR. Annual average gross alpha values from the TTR monitoring stations are higher than values from the surrounding CEMP stations. Annual average gross beta values from the TTR monitoring stations are generally lower than values observed for the surrounding CEMP stations. This may be due to use of sample filters with larger pore space because when glass-fiber filters began to be used at TTR Station 400, gross beta values increased. Gamma spectroscopy typically identified only naturally occurring radionuclides. The radionuclides cesium-134 and -137 were identified in only two samples at each station collected in the weeks following the destruction of the nuclear power reactor in Fukushima, Japan, on March 11, 2011. Observed gamma energy values never exceeded the local background by more than 4 μR/h. The higher observed gamma values were coincident with wind from any of the cardinal directions, which suggests that there is no significant transport from the Clean Slate contamination areas. Annual average daily gamma values at the TTR stations are higher than at the surrounding CEMP stations, but they are equivalent to or just slightly higher than the background estimates made at locations at equivalent elevations, such as Denver, Colorado. Winds in excess of approximately 15 mph begin to resuspend soil particles and create dust, but dust generation is also affected by soil temperature, relative humidity, and soil water content. Power curves provide good predictive equations for dust concentration as a function of wind speed. However, winds in the highest wind speed category occur infrequently.

54 ENVIRONMENTAL SCIENCES↗

2015 Assessment of Mercury in the Savannah River Site Environment and Responses to the Agency for Toxic Substances and Disease Registry 2012 Report on Assessment of Biota Exposure to Mercury Originating from the Savannah River Site

The purpose of this report is to 1) update previous Savannah River National Laboratory (SRNL) assessment reports (Kvartek et al. 1994 and Halverson et al. 2008) on the fate of mercury in the Savannah River Site (SRS) environment and 2) address comments and recommendations from the review of SRS by the Agency for Toxic Substances and Disease Registry (ATSDR) concerning the evaluation of exposures to contaminants in biota originating from the SRS. The ATSDR reviewed and evaluated data from SRS, South Carolina Department of Health & Environmental Control (SCDHEC) and the Georgia Department of Natural Resources (GDNR) concerning the non-radioactive contaminant mercury. This report will provide a response and update to conclusions and recommendations made by the ATSDR.

54 ENVIRONMENTAL SCIENCES↗

2014 Annual Summary Report for the Area 3 and Area 5 Radioactive Waste Management Sites at the Nevada National Security Site, Nye County, Nevada: Review of the Performance Assessments and Composite Analyses

This report summarizes the results and conclusions of the annual review of the performance assessments (PAs) and composite analyses (CAs) of the Area 3 and Area 5 Radioactive Waste Management Sites (RWMSs). The Area 3 and Area 5 RWMSs were issued Disposal Authorization Statements (DASs) in accordance with U.S. Department of Energy Order DOE O 435.1 “Radioactive Waste Management” (U.S. Department of Energy [DOE] 2001). The Area 3 RWMS and Area 5 RWMS DASs (DOE 1999a, 2000) require preparation of an annual summary report and a determination of the continuing adequacy of the PAs and CAs. The requirement to prepare an annual summary report is implemented in the Maintenance Plan for the PAs and CAs (National Security Technologies, LLC [NSTec] 2007a). The annual summary report is submitted to the DOE Office of Environmental Management for review and approval.

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FY 2017 Annual Summary Report for the Area 3 and Area 5 Radioactive Waste Management Sites at the Nevada National Security Site, Nye County, Nevada

The U.S. Department of Energy Standard Disposal Authorization Statement and Tank Closure Documentation (DOE-STD-5002-2017) requires preparation of an Annual Summary Report comparing low-level radioactive waste disposal facilities operations with the Disposal Authorization Statement (DAS) and its supporting technical documents including the performance assessment (PA) and the composite analysis (CA). The fiscal year (FY) 2017 Annual Summary Report for the Area 3 and Area 5 Radioactive Waste Management Sites (RWMSs) was prepared by reviewing planned and discovered changes, waste receipts, monitoring results, research and development (R&D) results, and the status of DAS conditions and issues to assess the validity of the DASs, PAs, and CAs.

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Scat Detection Dog Surveys for the San Joaquin Kit Fox on the Lawrence Livermore National Laboratory's Experimental Test Site (Site 300) and the Corral Hollow Ecological Reserve, Alameda and San Joaquin Counties, California

The San Joaquin kit fox (Vulpes macrotis mutica) historically occupied a large range in the San Joaquin Valley, California; however, their populations and habitat have since been significantly reduced by human impacts. More than 95% of the potential habitat for kit foxes on the San Joaquin Valley floor has been converted to irrigated agriculture, urbanized, or industrialized. The San Joaquin kit fox was listed as endangered by the USFWS in 1967 and as threatened by the State of California in 1971. In 2018, Working Dogs for Conservation (WD4C) was contracted by LLNS to provide professional conservation detection dog teams to survey for scats of San Joaquin kit fox on Site 300 and the neighboring California Department of Fish and Wildlife's Corral Hollow Ecological Reserve. The purpose of the surveys was to obtain updated information on kit fox presence on these properties.

54 ENVIRONMENTAL SCIENCES↗

Analysis of Dust Samples Collected from an Inland ISFSI Site (''Site B'').

In October of 2020, dust samples were collected from the surface of spent nuclear fuel (SNF) dry storage canisters during an inspection at an inland Independent Spent Fuel Storage Installation, the second inland site at which surface deposits have been sampled. The purpose of the sampling was to assess the composition and abundance of the soluble salts present on the canister surface, information which provides a metric for potential corrosion risks. The samples were delivered to Sandia National laboratories for analysis. At Sandia, the soluble salts were leached from the dust and quantified by ion chromatography. In addition, subsamples of the dust were taken for scanning electron microscopy to determine the texture and mineralogy of the dust and salts. The results of those analyses are presented in this report.

11 NUCLEAR FUEL CYCLE AND FUEL MATERIALS↗

A Section 110 Evaluation of Three Sites Associated with the Grable Test, Area 5, Nevada National Security Site, Nye County, Nevada

This report presents the results of a Section 110 recordation of the 280mm Atomic Gun emplacement and command post (CP) (26NY16244), an associated Flash Observation Point that was used to refine the gun’s firing range (26NY16243), and troop trenches used for Exercise Desert Rock V (26NY16242). All three of these resources are directly associated with the Grable test, a specific test in the Upshot-Knothole series of aboveground nuclear detonations. The Grable test occurred at 8:30 a.m. on May 25, 1953, in Area 5 of the Nevada National Security Site (NNSS). Desert Research Institute (DRI) recommends 26NY16244 and 26NY16242 eligible for listing in the National Register of Historic Places (NRHP) under a combination of the Secretary of the Interior’s Significance Criteria A and D at the national level of significance and related to the era of Nuclear Testing and the Cold War. All three of these resources are recommended as contributing elements to the Frenchman Flat Historic District (SHPO Resource No. D204).

280mm Atomic Gun↗

Composite Analysis Vadose Zone Transport of Selected Hanford Site Composite Analysis: Vadose Zone Transport of Selected Radionuclides Released from DOE O 435.1 Waste Sites

The main objectives of the composite analysis (CA) compliance case vadose zone (VZ) facet are to simulate transport of radionuclide releases from the surface to the water table, and to provide radionuclide transfer rates to groundwater for inclusion into the Plateau-to-River (P2R) saturated zone (SZ) model (CP-57037, Model Package Report: Plateau to River Groundwater Model, Version 8.3). The parallel exascale version of the Subsurface Transport Over Multiple Phases (eSTOMP) simulator is used to simulate flow and transport for the CA VZ models. The CA compliance case approach to address the scale and distribution of contaminant sources in the VZ facet is to subdivide the Hanford Site Central Plateau into 26 models that contain contaminant sources and liquid discharges likely to commingle during migration through the VZ to the water table.

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Modification Request to Establish Use Restrictions for Three Corrective Action Sites in Corrective Action Unit 5000: Archived – Corrective Action Sites, in Appendix IV of the Federal Facility Agreement and Consent Order (FFACO)

The U.S. Department of Energy, National Nuclear Security Administration Nevada Field Office (NNSA/NFO) requests that the Nevada Division of Environmental Protection (NDEP) approve the enclosed modification to establish URs for the following CASs in CAU 5000: Archived - Archived Corrective Action Sites, in Appendix IV of the FFACO.

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Lawrence Livermore National Laboratory Experimental Test Site, Site 300: Building 843 Corp Yard Redesign Soil Sampling and Analysis Plan (June 2023)

This Soil Sampling and Analysis Plan (SAP) was prepared by the Environmental Functional Area (EFA)/Technical Services Department (TSD) of the Environment, Safety & Health (ES&H) Directorate for the Project Management Office (PMO) for the proposed Building 843 (B843) Corp Yard Redesign project (project). The purpose of the SAP was to identify chemicals of concern, describe the procedures for collection and analysis of environmental samples, and evaluation of analytical data (chemical and radiological) to determine management options of excavated soil during project construction. This SAP follows the criteria established in Lawrence Livermore National Laboratory’s (LLNL’s) Soils Screening and Management Plan (SSMP) (LLNL 2022), which is consistent with U.S. Environmental Protection Agency (EPA) guidance for developing Data Quality Objectives for environmental data (EPA 2006) and the Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) guidance (U.S. NRC, U.S. EPA, U.S. DOE, U.S. DOD 2000). The scope of this SAP is based on the B843 Corp Yard Redesign drawing set dated December 3, 2021.

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Lawrence Livermore National Laboratory Experimental Test Site, Site 300: S300 Roadway Improvements - 854 Complex Soil Sampling and Analysis Plan (June 2023)

This Soil Sampling and Analysis Plan (SAP) was prepared by the Environmental Functional Area (EFA)/Technical Services Department (TSD) of the Environment, Safety & Health (ES&H) Directorate for the Project Management Office (PMO) for the proposed Roadway Improvements Project at the Building 854 Complex (project). The purpose of the SAP was to identify chemicals of concern, describe the procedures for collection and analysis of environmental samples, and evaluation of analytical data (chemical and radiological) to determine management options of excavated soil during project construction. This SAP follows criteria established in Lawrence Livermore National Laboratory’s (LLNL) Soils Screening and Management Plan (SSMP) (LLNL 2022), which was formalized in accordance with U.S. Environmental Protection Agency (EPA) guidance for developing Data Quality Objectives for environmental data (EPA 2006) and the Multi-Agency Radiation Survey and Site Investigation Manual (MARSSIM) guidance (U.S. NRC, U.S. EPA, U.S. DOE, and U.S. DOD 2000). The scope of this SAP is based on preliminary design information provided by PMO.

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The Niwot Ridge Subalpine Forest US-NR1 AmeriFlux site – Part 1: Data acquisition and site record-keeping

Abstract. The Niwot Ridge Subalpine Forest AmeriFlux site (US-NR1) has been measuring eddy-covariance ecosystem fluxes of carbon dioxide, heat, and water vapor since 1 November 1998. Throughout this 17-year period there have been changes to the instrumentation and improvements to the data acquisition system. Here, in Part 1 of this three-part series of papers, we describe the hardware and software used for data-collection and metadata documentation. We made changes to the data acquisition system that aimed to reduce the system complexity, increase redundancy, and be as independent as possible from any network outages. Changes to facilitate these improvements were (1) switching to a PC/104-based computer running the National Center for Atmospheric Research (NCAR) In-Situ Data Acquisition Software (NIDAS) that saves the high-frequency data locally and over the network, and (2) time-tagging individual 10 Hz serial data samples using network time protocol (NTP) coupled to a GPS-based clock, providing a network-independent, accurate time base. Since making these improvements almost 2 years ago, the successful capture of high-rate data has been better than 99.98 %. We also provide philosophical concepts that shaped our design of the data system and are applicable to many different types of environmental data collection.

54 ENVIRONMENTAL SCIENCES↗

Extensive site-directed mutagenesis reveals interconnected functional units in the alkaline phosphatase active site

Enzymes enable life by accelerating reaction rates to biological timescales. Conventional studies have focused on identifying the residues that have a direct involvement in an enzymatic reaction, but these so-called ‘catalytic residues’ are embedded in extensive interaction networks. Although fundamental to our understanding of enzyme function, evolution, and engineering, the properties of these networks have yet to be quantitatively and systematically explored. We dissected an interaction network of five residues in the active site of Escherichia coli alkaline phosphatase. Analysis of the complex catalytic interdependence of specific residues identified three energetically independent but structurally interconnected functional units with distinct modes of cooperativity. From an evolutionary perspective, this network is orders of magnitude more probable to arise than a fully cooperative network. From a functional perspective, new catalytic insights emerge. Further, such comprehensive energetic characterization will be necessary to benchmark the algorithms required to rationally engineer highly efficient enzymes.

59 BASIC BIOLOGICAL SCIENCES↗

Survey of Aquatic Turtles on the Savannah River Site, South Carolina, USA, for Prevalence of Ranavirus

Ranaviruses have the ability to infect amphibians, fish, and reptiles, and have caused multiple amphibian die-off events in the United States (U. S.) and Europe. Furthermore, their prevalence in amphibian populations is much more commonly studied than in chelonian populations. We examined blood samples (N=286) from eight aquatic turtle species collected during 2008-2014 on the Savannah River Site (SRS), South Carolina as part of long-term mark-recapture efforts. Previous studies in the southeastern US found high prevalence of ranavirus in amphibians, but we did not detect ranavirus in any of the turtles sampled, suggesting low presence, prevalence, or both in aquatic turtles across the SRS during the years tested.

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