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At least 577 records · Page 32
All sourcesNASA NTRSDOE OSTI
NASA NTRS1995

Heart rate and respiratory rhythm dynamics on ascent to high altitude

OBJECTIVE--To investigate the alterations in autonomic control of heart rate at high altitude and to test the hypothesis that hypoxaemic stress during exposure to high altitude induces non-linear, periodic heart rate oscillations, similar to those seen in heart failure and the sleep apnoea syndrome. SUBJECTS--11 healthy subjects aged 24-64. MAIN OUTCOME MEASURES--24 hour ambulatory electrocardiogram records obtained at baseline (1524 m) and at 4700 m. Simultaneous heart rate and respiratory dynamics during 2.5 hours of sleep by fast Fourier transform analysis of beat to beat heart rate and of an electrocardiographically derived respiration signal. RESULTS--All subjects had resting hypoxaemia at high altitude, with an average oxyhaemoglobin saturation of 81% (5%). There was no significant change in mean heart rate, but low frequency (0.01-0.05 Hz) spectral power was increased (P < 0.01) at high altitude. Time series analysis showed a complex range of non-linear sinus rhythm dynamics. Striking low frequency (0.04-0.06 Hz) heart rate oscillations were observed during sleep in eight subjects at high altitude. Analysis of the electrocardiographically derived respiration signal indicated that these heart rate oscillations correlated with low frequency respiratory oscillations. CONCLUSIONS--These data suggest (a) that increased low frequency power during high altitude exposure is not simply attributable to increased sympathetic modulation of heart rate, but relates to distinctive cardiopulmonary oscillations at approximately 0.05 Hz and (b) that the emergence of periodic heart rate oscillations at high altitude is consistent with an unstable cardiopulmonary control system that may develop on acute exposure to hypoxaemic stress.

Non-NASA Center↗
NASA NTRS1995

Reliability of psychophysiological responses across multiple motion sickness stimulation tests

Although there is general agreement that a high degree of variability exists between subjects in their autonomic nervous system responses to motion sickness stimulation, very little evidence exists that examines the reproducibility of autonomic responses within subjects during motion sickness stimulation. Our objectives were to examine the reliability of autonomic responses and symptom levels across five testing occasions using the (1) final minute of testing, (2) change in autonomic response and the change in symptom level, and (3) strength of the relationship between the change in symptom level and the change in autonomic responses across the entire motion sickness test. The results indicate that, based on the final minute of testing, the autonomic responses of heart rate, blood volume pulse, and respiration rate are moderately stable across multiple tests. Changes in heart rate, blood volume pulse, respiration rate, and symptoms throughout the test duration are less stable across the tests. Finally, autonomic responses and symptom levels are significantly related across the entire motion sickness test.

NASA Discipline Neuroscience↗
NASA NTRS2004

Redesign of the Human Metabolic Simulator

The National Aeronautics and Space Administration (NASA) is currently building a Human Metabolic Simulator (HMS) at the Johnson Space Center as part of the Advanced Life Support Air Revitalization Technology Evaluation Facility (ARTEF). The purpose of ARTEF is to evaluate Environmental Control and Life Support System Technologies for Advanced Missions. The HMS is needed to reproduce the primary metabolic effects of human respiration on an enclosed atmosphere when humans cannot be present and the impact of human presence on the system is required. A HMS was designed, built and successfully operated in 2000 but larger crew size requirements and the expense of upgrade of the current system necessitate redesign. This paper addresses the redesign. Several concepts were considered, ranging from chemical oxidation of a hydrocarbon like ethanol or ethyl acetate to carbon dioxide and water, oxidation of an iron-containing compound, or by using a fuel cell. For reasons of cost, simplicity, safety and other factors, the concept chosen includes: a molecular sieve packaged as an industrial oxygen concentrator to remove oxygen from the atmosphere, with direct carbon dioxide, water and heat injection. The water injection is done via heating water to steam with a heat exchanger and thermal effects are handled by directly adding heat to the air stream with a second heat exchanger. Both heat exchangers are supplied by a hot oil loop. The amount of oxygen removal, carbon dioxide addition, water addition and heat addition were calculated using metabolic profiles for respiration and heat, calculated using a series of empirical equations developed for International Space Station (ISS). Sketches of the Human Metabolic Simulator and the hot oil bath loop used to supply heat to the heat exchangers are included

Duffield, Bruce↗
NASA NTRS2007

Toxicity of Carbon Nanotubes and Its Implications for Occupational and Environmental Health

Carbon nanotubes (CNTs), which possess desirable electrical and mechanical properties, potentially have wide industrial applications. CNTs exist in two forms, single-wall (SW) and multi-wall (MW). There has been great concern that if CNTs enter the work environment as suspended respirable particulate matter (PM), they could pose an inhalation hazard. The results of recent rodent studies have collectively shown that CNTs can produce inflammation, epithelioid granulomas, fibrosis, and biochemical changes in the lungs. Studies in mice given equal amounts of test dusts showed that CNTs were more toxic than quartz and produced lesions that became progressively more pronounced. These results have led us to recommend that respirable CNT dust be considered a serious occupational health hazard, and that exposure limits be established in the expectation of expanded industrial applications. CNTs, which are totally insoluble and fibrous, would be expected to be more biopersistent than mineral fibers. Biopersistence is the key factor determining the long-term toxicity of mineral fibers and certainly of CNTs too. We have postulated that the electrical and fibrous properties of CNTs also play important roles in the toxicity of CNTs in the lungs. Recently, MWCNTs have been found in ultrafine PM aggregates in combustion streams of methane, propane, and natural-gas flames of typical stoves; indoor and outdoor fine (< 2.5 micron) PM samples were reported to contain significant fractions of MWCNTs. Environmental fine PM is mainly formed from combustion of fuels, and fine PM has been reported to be a major contributor to the induction of cardiopulmonary diseases by pollutants. Given that manufactured SWCNTs and/or MWCNTs have elicited pathological changes in the lungs and heart, we have postulated that exposure to combustion-generated MWCNTs in fine PM in the air may play a significant role in air pollution-related cardiopulmonary diseases. Therefore, CNTs from manufacturing and combustion sources in the environment could have adverse effects on human health.

Lam, Chiu-wing↗
NASA NTRS2007

STS 118 Return Samples: Assessment of Air Quality aboard the Shuttle (STS-118) and International Space Station

The toxicological assessments of 2 grab sample canisters (GSCs) and one pair of formaldehyde badges from the Shuttle are reported. Analytical methods have not changed from earlier reports. The recoveries of the 3 surrogates (C-13-acetone, fluorobenzene, and chlorobenzene) from the 2 GSCs averaged 120, 117, and 122 %, respectively. Three formaldehyde controls averaged 98% recovery. The Shuttle atmosphere was acceptable for human respiration. The toxicological assessment of 8 GSCs and 6 pairs of formaldehyde badges from the ISS is shown. The recoveries of the 3 standards (as listed above) from the GSCs averaged 99, 99 and 99%, respectively. Three formaldehyde control badges averaged 98% recovery. Based on these limited samples, the ISS atmosphere is acceptable for human respiration. The alcohol levels were well controlled throughout the period of sampling.

James, John T.↗
NASA NTRS2007

Portable Unit for Metabolic Analysis

The Portable Unit for Metabolic Analysis (PUMA) is an instrument that measures several quantities indicative of human metabolic function. Specifically, this instrument makes time-resolved measurements of temperature, pressure, flow, and the partial pressures of oxygen and carbon dioxide in breath during both inhalation and exhalation. Portable instruments for measuring these quantities have been commercially available, but the response times of those instruments are too long to enable temporal resolution of phenomena on the time scales of human respiration cycles. In contrast, the response time of the PUMA is significantly shorter than characteristic times of human respiration phenomena, making it possible to analyze varying metabolic parameters, not only on sequential breath cycles but also at successive phases of inhalation and exhalation within the same breath cycle. In operation, the PUMA is positioned to sample breath near the subject s mouth. Commercial off-the-shelf sensors are used for three of the measurements: a miniature pressure transducer for pressure, a thermistor for temperature, and an ultrasonic sensor for flow. Sensors developed at Glenn Research Center are used for measuring the partial pressures of oxygen and carbon dioxide: The carbon dioxide sensor exploits the relatively strong absorption of infrared light by carbon dioxide. Light from an infrared source passes through the stream of inhaled or exhaled gas and is focused on an infrared- sensitive photodetector. The oxygen sensor exploits the effect of oxygen in quenching the fluorescence of ruthenium-doped organic molecules in a dye on the tip of an optical fiber. A blue laser diode is used to excite the fluorescence, and the optical fiber carries the fluorescent light to a photodiode, the temporal variation of the output of which bears a known relationship with the rate of quenching of fluorescence and, hence, with the partial pressure of oxygen. The outputs of the sensors are digitized, preprocessed by a small onboard computer, and then sent wirelessly to a desktop computer, where the collected data are analyzed and displayed. In addition to the raw data on temperature, pressure, flow, and mole fractions of oxygen and carbon dioxide, the display can include volumetric oxygen consumption, volumetric carbon dioxide production, respiratory equivalent ratio, and volumetric flow rate of exhaled gas.

Dietrich, Daniel L.↗
NASA NTRS2011

International Space Station (ISS) Emergency Mask (EM) Development

The Emergency Mask (EM) is considered a secondary response emergency Personal Protective Equipment (PPE) designed to provide respiratory protection to the International Space Station (ISS) crewmembers in response to a post-fire event or ammonia leak. The EM is planned to be delivered to ISS in 2012 to replace the current air purifying respirator (APR) onboard ISS called the Ammonia Respirator (AR). The EM is a one ]size ]fits ]all model designed to fit any size crewmember, unlike the APR on ISS, and uses either two Fire Cartridges (FCs) or two Commercial Off-the-Shelf (COTS) 3M(Trademark). Ammonia Cartridges (ACs) to provide the crew with a minimum of 8 hours of respiratory protection with appropriate cartridge swap ]out. The EM is designed for a single exposure event, for either post ]fire or ammonia, and is a passive device that cannot help crewmembers who cannot breathe on their own. The EM fs primary and only seal is around the wearer fs neck to prevent a crewmember from inhaling contaminants. During the development of the ISS Emergency Mask, several design challenges were faced that focused around manufacturing a leak free mask. The description of those challenges are broadly discussed but focuses on one key design challenge area: bonding EPDM gasket material to Gore(Registered Trademark) fabric hood.

Toon, Katherine P.↗
NASA NTRS2010

Proposed Objective Odor Control Test Methodology for Waste Containment

The Orion Cockpit Working Group has requested that an odor control testing methodology be proposed to evaluate the odor containment effectiveness of waste disposal bags to be flown on the Orion Crew Exploration Vehicle. As a standardized "odor containment" test does not appear to be a matter of record for the project, a new test method is being proposed. This method is based on existing test methods used in industrial hygiene for the evaluation of respirator fit in occupational settings, and takes into consideration peer reviewed documentation of human odor thresholds for standardized contaminates, industry stardnard atmostpheric testing methodologies, and established criteria for laboratory analysis. The proposed methodology is quantitative, though it can readily be complimented with a qualitative subjective assessment. Isoamyl acetate (IAA - also known at isopentyl acetate) is commonly used in respirator fit testing, and there are documented methodologies for both measuring its quantitative airborne concentrations. IAA is a clear, colorless liquid with a banana-like odor, documented detectable smell threshold for humans of 0.025 PPM, and a 15 PPB level of quantation limit.

Vos, Gordon↗
NASA NTRS2014

Spring Hydrology Determines Summer Net Carbon Uptake in Northern Ecosystems

Increased photosynthetic activity and enhanced seasonal CO2 exchange of northern ecosystems have been observed from a variety of sources including satellite vegetation indices (such as the Normalized Difference Vegetation Index; NDVI) and atmospheric CO2 measurements. Most of these changes have been attributed to strong warming trends in the northern high latitudes (greater than or equal to 50N). Here we analyze the interannual variation of summer net carbon uptake derived from atmospheric CO2 measurements and satellite NDVI in relation to surface meteorology from regional observational records. We find that increases in spring precipitation and snow pack promote summer net carbon uptake of northern ecosystems independent of air temperature effects. However, satellite NDVI measurements still show an overall benefit of summer photosynthetic activity from regional warming and limited impact of spring precipitation. This discrepancy is attributed to a similar response of photosynthesis and respiration to warming and thus reduced sensitivity of net ecosystem carbon uptake to temperature. Further analysis of boreal tower eddy covariance CO2 flux measurements indicates that summer net carbon uptake is positively correlated with early growing-season surface soil moisture, which is also strongly affected by spring precipitation and snow pack based on analysis of satellite soil moisture retrievals. This is attributed to strong regulation of spring hydrology on soil respiration in relatively wet boreal and arctic ecosystems. These results document the important role of spring hydrology in determining summer net carbon uptake and contrast with prevailing assumptions of dominant cold temperature limitations to high-latitude ecosystems. Our results indicate potentially stronger coupling of boreal/arctic water and carbon cycles with continued regional warming trends.

Carbon Uptake↗
NASA NTRS2015

NASA Developments in Personnel Protective Equipment

NASA has some unique and challenging PPE needs: there are credible threats to air quality (fire, ammonia leak, hydrazine leak)that require a contingency breathing apparatus that operates for many hours - but there is not enough space or up-mass to provide supplied air tanks. We cannot use "Scott Air Tanks" commonly used by firefighters and other first responders. NASA has developed a respirator based emergency breathing device. It uses a "one size fits everybody in the astronaut corps" hooded mask with excellent chemical permeability and fire resistance properties, and a filtering respirator cartridge that protects the wearer from ammonia leaks, hydrazine leaks, or products of combustion. If you need a small, lightweight emergency breathing system that lasts longer than a supplied air system, we should meet and learn if NASA sponsored technology development can help.

Graf, John↗
NASA NTRS2002

Analysis and Design of Crew Sleep Station for ISS

This paper details the analysis and design of the Temporary Sleep Station (TeSS) environmental control system for International Space Station (ISS). The TeSS will provide crewmembers with a private and personal space, to accommodate sleeping, donning and doffing of clothing, personal communication and performance of recreational activities. The need for privacy to accommodate these activities requires adequate ventilation inside the TeSS. This study considers whether temperature, carbon dioxide, and humidity within the TeSS remain within crew comfort and safety levels for various expected operating scenarios. Evaluation of these scenarios required the use and integration of various simulation codes. An approach was adapted for this study, whereby results from a particular code were integrated with other codes when necessary. Computational Fluid Dynamics (CFD) methods were used to evaluate the flow field inside the TeSS, from which local gradients for temperature, velocity, and species concentration such as CO (sub 2) could be determined. A model of the TeSS, containing a human, as well as equipment such as a laptop computer, was developed in FLUENT, a finite-volume code. Other factors, such as detailed analysis of the heat transfer through the structure, radiation, and air circulation from the TeSS to the US Laboratory Aisle, where the TeSS is housed, were considered in the model. A complementary model was developed in G189A, a code which has been used by NASA/JSC for environmental control systems analyses since the Apollo program. Boundary conditions were exchanged between the FLUENT and G189A TeSS models. G189A provides human respiration rates to the FLUENT model, while the FLUENT model provides local convective heat transfer coefficients to G189A model. An additional benefit from using an approach with both a systems simulation and CFD model, is the capability to verify the results of each model by comparison to the results of the other model. The G189A and FLUENT models were used to evaluate various ventilation designs for the TeSS over a range of operating conditions with varying crew metabolic load, equipment operating modes, ventilation flow rates, and with the TeSS doors open and closed. Results from the study were instrumental in the optimization of a design for the TeSS ventilation hardware. A special case was considered where failure of the TeSS ventilation system occurred. In this case, a study was conducted in order to determine the time required for the CO (sub 2) concentration inside the TeSS to increase to ISS limit values under transient conditions. A lumped-capacitance code, SINDA-FLUINT was used in this case to provide accurate predictions of the human reaction to the TeSS cabin conditions including core and skin temperatures and body heat storage. A simple two-dimensional CFD model of a crewmember inside the TeSS was developed in FLUENT in order to determine the volume envelope of the respired air from the human, which maintained a minimum velocity profile. This volume was then used in the SINDA-FLUINT model to facilitate the calculations of CO (sub 2) concentrations, dry bulb temperatures and humidity levels inside the TeSS.

Keener, John F.↗
NASA NTRS2015

Simulated Impacts of Climate Change on Water Use and Yield of Irrigated Sugarcane in South Africa

Reliable predictions of climate change impacts on water use, irrigation requirements and yields of irrigated sugarcane in South Africa (a water-scarce country) are necessary to plan adaptation strategies. Although previous work has been done in this regard, methodologies and results vary considerably. The objectives were (1) to estimate likely impacts of climate change on sugarcane yields, water use and irrigation demand at three irrigated sugarcane production sites in South Africa (Malelane, Pongola and La Mercy) for current (1980-2010) and future (2070-2100) climate scenarios, using an approach based on the Agricultural Model Inter-comparison and Improvement Project (AgMIP) protocols; and (2) to assess the suitability of this methodology for investigating climate change impacts on sugarcane production. Future climate datasets were generated using the Delta downscaling method and three Global Circulation Models (GCMs) assuming atmospheric CO2 concentration [CO2] of 734 ppm(A2 emissions scenario). Yield and water use were simulated using the DSSAT-Canegro v4.5 model. Irrigated cane yields are expected to increase at all three sites (between 11 and 14%), primarily due to increased interception of radiation as a result of accelerated canopy development. Evapotranspiration and irrigation requirements increased by 11% due to increased canopy cover and evaporative demand. Sucrose yields are expected to decline because of increased consumption of photo-assimilate for structural growth and maintenance respiration. Crop responses in canopy development and yield formation differed markedly between the crop cycles investigated. Possible agronomic implications of these results include reduced weed control costs due to shortened periods of partial canopy, a need for improved efficiency of irrigation to counter increased demands, and adjustments to ripening and harvest practices to counter decreased cane quality and optimize productivity. Although the Delta climate data downscaling method is considered robust, accurate and easily-understood, it does not change the future number of rain-days per month. The impacts of this and other climate data simplifications ought to be explored in future work. Shortcomings of the DSSAT-Canegro model include the simulated responses of phenological development, photosynthesis and respiration processes to high temperatures, and the disconnect between simulated biomass accumulation and expansive growth. Proposed methodology refinements should improve the reliability of predicted climate change impacts on sugarcane yield.

Climate change↗
NASA NTRS2016

Soil Moisture Active Passive Mission L4_C Data Product Assessment (Version 2 Validated Release)

The SMAP satellite was successfully launched January 31st 2015, and began acquiring Earth observation data following in-orbit sensor calibration. Global data products derived from the SMAP L-band microwave measurements include Level 1 calibrated and geolocated radiometric brightness temperatures, Level 23 surface soil moisture and freezethaw geophysical retrievals mapped to a fixed Earth grid, and model enhanced Level 4 data products for surface to root zone soil moisture and terrestrial carbon (CO2) fluxes. The post-launch SMAP mission CalVal Phase had two primary objectives for each science product team: 1) calibrate, verify, and improve the performance of the science algorithms, and 2) validate accuracies of the science data products as specified in the L1 science requirements. This report provides analysis and assessment of the SMAP Level 4 Carbon (L4_C) product pertaining to the validated release. The L4_C validated product release effectively replaces an earlier L4_C beta-product release (Kimball et al. 2015). The validated release described in this report incorporates a longer data record and benefits from algorithm and CalVal refinements acquired during the SMAP post-launch CalVal intensive period. The SMAP L4_C algorithms utilize a terrestrial carbon flux model informed by SMAP soil moisture inputs along with optical remote sensing (e.g. MODIS) vegetation indices and other ancillary biophysical data to estimate global daily net ecosystem CO2 exchange (NEE) and component carbon fluxes for vegetation gross primary production (GPP) and ecosystem respiration (Reco). Other L4_C product elements include surface (10 cm depth) soil organic carbon (SOC) stocks and associated environmental constraints to these processes, including soil moisture and landscape freeze/thaw (FT) controls on GPP and respiration (Kimball et al. 2012). The L4_C product encapsulates SMAP carbon cycle science objectives by: 1) providing a direct link between terrestrial carbon fluxes and underlying FT and soil moisture constraints to these processes, 2) documenting primary connections between terrestrial water, energy and carbon cycles, and 3) improving understanding of terrestrial carbon sink activity in northern ecosystems. There are no L1 science requirements for the L4_C product; however self-imposed requirements have been established focusing on NEE as the primary product field for validation, and on demonstrating L4_C accuracy and success in meeting product science requirements (Jackson et al. 2012). The other L4_C product fields also have strong utility for carbon science applications; however, analysis of these other fields is considered secondary relative to primary validation activities focusing on NEE. The L4_C targeted accuracy requirements are to meet or exceed a mean unbiased accuracy (ubRMSE) for NEE of 1.6 g C/sq m/d or 30 g C/sq m/yr, emphasizing northern (45N) boreal and arctic ecosystems; this is similar to the estimated accuracy level of in situ tower eddy covariance measurement-based observations (Baldocchi 2008).

Cal/Val↗
NASA NTRS2015

Improving the Monitoring of Crop Productivity Using Spaceborne Solar-Induced Fluorescence

Large-scale monitoring of crop growth and yield has important value for forecasting food production and prices and ensuring regional food security. A newly emerging satellite retrieval, solar-induced fluorescence (SIF) of chlorophyll, provides for the first time a direct measurement related to plant photosynthetic activity (i.e. electron transport rate). Here, we provide a framework to link SIF retrievals and crop yield, accounting for stoichiometry, photosynthetic pathways, and respiration losses. We apply this framework to estimate United States crop productivity for 2007-2012, where we use the spaceborne SIF retrievals from the Global Ozone Monitoring Experiment-2 satellite, benchmarked with county-level crop yield statistics, and compare it with various traditional crop monitoring approaches. We find that a SIF-based approach accounting for photosynthetic pathways (i.e. C3 and C4 crops) provides the best measure of crop productivity among these approaches, despite the fact that SIF sensors are not yet optimized for terrestrial applications. We further show that SIF provides the ability to infer the impacts of environmental stresses on autotrophic respiration and carbon-use-efficiency, with a substantial sensitivity of both to high temperatures. These results indicate new opportunities for improved mechanistic understanding of crop yield responses to climate variability and change.

Guan, Kaiyu↗
NASA NTRS2016

Retrieval of Daily Maps of PM2.5 Aerosol in the Problematic California Valleys: Bright, Speckled Reflectances, Thin AOT, but High Pollution

The San Joaquin Valley suffers from severe episodes of respirable aerosol (PM2.5) in wintertime.We provide maps of aerosol episodes using daily snapshots of PM2.5 and its changing features despite numerous difficulties inherent to sampling the region. Linear relationships relating aerosol optical thickness (AOT) to PM2.5 give an explained variance of approximately 3.The GEO-CAPE mission has as a goal the provision of relevant measures of respirable aerosol to the community,but has not formulated a science goal beyond the limited goal of retrieval of AOT, bringing the usefulness of GEO-CAPE into doubt.Our special focus was on the DISCOVER-AQ period, Jan-Feb 2013, which had many supporting measurements.Both high pollution and retrieval difficulties tend to occur in many Mediterranean agricultural regions like the San Joauin. One difficulty is the relatively bright surfaces with considerable exposed soil. NASAs MAIAC and MODIS Deep Blue retrieval techniques are shown to have considerable skill even at low aerosol optical thickness (AOT) values, as evaluated by concurrent AERONET sunphotometer measurements.More significantly, these AOT values can correspond to high daytime PM2.5 since aerosol mixed layer depth is thin and variable, 200m 600 m. The thin layers derive from typical subsidence of dry air between more stormy periods. This situation provides an advantage: water vapor column is also almost completely limited to a similar mixed layer depth, and can thus serve as a measure of aerosol dilution.Using the MAIAC Water Vapor Column:In order to make the maps below, we used the MAIAC data but subtracted partial water-vapor columns estimated from MERRA Reanalysis Data availabe from the GSFC GMAO using kriging. We did not use the mixed-layer estimates from MERRA, since such analyses were found problematic during our forecasting exercises for DISCOVER-AQ. Observations from the aircraft soundings suggested that this overlying moisture was mostly due to larger scale flows, not ML venting.However, the specific humidity at the surface and a nearly well-mixed ML was analyzed by kriging from the surface network (MesoWest, University of Utah). These were thought to be truer, uninfluenced by physical process modeling that combines with data observations. (TBD: How different are they?) Procedure: Subtract overlying partial water columns from MAIAC column water and divide this by a surface value of water vapor. (MAIAC column is expressed in cm of water, i.e., water vapor at surface conditions.This method appears to bring out useful details in the distribution of submicron particles in the very problematic Wintertime San Joaquin Valley, and allow analysis of pollution episodes throughout the valley, rather than long-term averages.

san joaquin valley↗
NASA NTRSDate not supplied

Investigating Biological Responses to Deep Space Radiation for Missions Beyond Low Earth Orbit (LEO) using Yeast

To enable long-term spaceflight missions and establish habitation on the Moon and Mars, we require a comprehensive understanding of the effects of chronic deep space radiation exposure on humans. BioSentinel is NASA’s first biological CubeSat to venture beyond Low Earth Orbit (LEO). It utilizes Saccharomyces cerevisiae (budding yeast) as a model organism to study biological responses to deep space radiation. Yeast share significant genetic homology with humans, including basic cellular metabolism and DNA repair mechanisms. In addition, unlike human cell cultures, yeast can survive the duration and constraints of a deep space mission. BioSentinel measures biological responses using an optical system and alamarBlue oxidation-reduction (redox) dye. Two strains of yeast are studied - a wild-type and a rad51 mutant strain that is deficient in DNA repair. Changes in metabolism and growth are monitored throughout the nominal 6-month mission. Preliminary tests indicate a significant change in the alamarBlue response to low-dose ionizing radiation (IR). Additionally, rad51 cells have shown an IR dose-dependent decrease in glucose uptake and accumulation of oxidized NADH (NAD+). These biomolecules are involved in reactions responsible for basic cell processes, including growth and development, signaling, and respiration. The current study expanded upon previous data by exposing yeast to deep space-relevant radiation. Glucose and NADH/NAD+ assays were conducted on yeast subjected to varying dosages of high-energy Fe-56 and simulated galactic cosmic rays (GCRs). The resulting data was analyzed using Excel and GraphPad Prism. A particular focus was to identify biomolecules resulting from aerobic respiration, which requires the presence of oxygen, or anaerobic processes. As long-term spaceflight missions draw near, it is increasingly important to characterize biological processes affected by the conditions of deep space. Studying biomolecular damage caused by deep space radiation may enable the development of engineering controls or biomedical therapeutics that mitigate health complications for future astronauts.

Kyra Keenan↗
NASA NTRSDate not supplied

Crowdsourcing for Innovative Personal Protection Technology

Personal protective equipment (PPE) plays a vital role in safeguarding workers from workplace hazards and minimizing exposure to harmful substances or conditions. To meet the changing needs and challenges faced by workers in various industries, PPE is continuously evolving. As new technologies emerge, advances in materials, design, and functionality contribute to PPE improvements. By incorporating personal protective technology (PPT) into traditional PPE, worker safety and well-being can be further enhanced. Crowdsourcing can harness a wide range of perspectives and expertise to identify novel technologies, materials, designs, or applications that can revolutionize PPE and improve worker protection. Partnering with the National Aeronautics and Space Administration (NASA) Center for Center of Excellence for Collaborative Innovation (CoECI), the National Institute for Occupational Safety and Health (NIOSH) National Personal Protection Technology Laboratory (NPPTL) launched three crowdsourcing challenges. These challenges aimed to promote innovation in several PPE areas: protective clothing, counterfeit respirators, and respirator fit. Preliminary results produced promising technologies and approaches promoting improved awareness, comfort, adaptability, and effectiveness against workplace hazards.

crowdsourcing↗
DOE OSTI2021

Data for "Soil texture and environmental conditions influence the biogeochemical responses of soils to drought and flooding"

This dataset contains data used for the paper "Soil texture and environmental conditions influence the biogeochemical responses of soils to drought and flooding". The Related References field will be updated with a full citation when available.Climate change is intensifying the global water cycle, with increased frequency of drought and flood. Water is an important driver of soil carbon dynamics, and it is crucial to understand how moisture disturbances will affect carbon availability and fluxes in soils. Here we investigate the role of water in substrate-microbe connectivity and soil carbon cycling under extreme moisture conditions. We collected soils from Alaska, Florida, and Washington USA, and incubated them under Drought and Flood conditions. Drought had a stronger effect on soil respiration, pore-water carbon, and microbial community composition than flooding. Soil response was not consistent across sites, and was influenced by site-level pedological and environmental factors. Soil texture and porosity can influence microbial access to substrates through the pore network, driving the chemical response. Further, the microbial communities are adapted to the historic stress conditions at their sites and therefore show site-specific responses to drought and flood.This dataset contains a compressed (.zip) archive of the data and R scripts used for this manuscript. The dataset includes files in .csv and .txt format, which can be accessed and processed using MS Excel or R. This archive can also be accessed on GitHub at https://github.com/kaizadp/TES_3Soils_2021 (DOI: 10.5281/zenodo.4792655).

54 ENVIRONMENTAL SCIENCES↗