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At least 577 records · Page 32

Genome-resolved metagenomics reveals site-specific diversity of episymbiotic CPR bacteria and DPANN archaea in groundwater ecosystems

Abstract Candidate phyla radiation (CPR) bacteria and DPANN archaea are unisolated, small-celled symbionts that are often detected in groundwater. The effects of groundwater geochemistry on the abundance, distribution, taxonomic diversity and host association of CPR bacteria and DPANN archaea has not been studied. Here, we performed genome-resolved metagenomic analysis of one agricultural and seven pristine groundwater microbial communities and recovered 746 CPR and DPANN genomes in total. The pristine sites, which serve as local sources of drinking water, contained up to 31% CPR bacteria and 4% DPANN archaea. We observed little species-level overlap of metagenome-assembled genomes (MAGs) across the groundwater sites, indicating that CPR and DPANN communities may be differentiated according to physicochemical conditions and host populations. Cryogenic transmission electron microscopy imaging and genomic analyses enabled us to identify CPR and DPANN lineages that reproducibly attach to host cells and showed that the growth of CPR bacteria seems to be stimulated by attachment to host-cell surfaces. Our analysis reveals site-specific diversity of CPR bacteria and DPANN archaea that coexist with diverse hosts in groundwater aquifers. Given that CPR and DPANN organisms have been identified in human microbiomes and their presence is correlated with diseases such as periodontitis, our findings are relevant to considerations of drinking water quality and human health.

59 BASIC BIOLOGICAL SCIENCES↗

Decomposing a San Francisco estuary microbiome using long-read metagenomics reveals species- and strain-level dominance from picoeukaryotes to viruses

ABSTRACT Although long-read sequencing has enabled obtaining high-quality and complete genomes from metagenomes, many challenges still remain to completely decompose a metagenome into its constituent prokaryotic and viral genomes. This study focuses on decomposing an estuarine metagenome to obtain a more accurate estimate of microbial diversity. To achieve this, we developed a new bead-based DNA extraction method, a novel bin refinement method, and obtained 150 Gbp of Nanopore sequencing. We estimate that there are ~500 bacterial and archaeal species in our sample and obtained 68 high-quality bins (>90% complete, <5% contamination, ≤5 contigs, contig length of >100 kbp, and all ribosomal and tRNA genes). We also obtained many contigs of picoeukaryotes, environmental DNA of larger eukaryotes such as mammals, and complete mitochondrial and chloroplast genomes and detected ~40,000 viral populations. Our analysis indicates that there are only a few strains that comprise most of the species abundances. IMPORTANCE Ocean and estuarine microbiomes play critical roles in global element cycling and ecosystem function. Despite the importance of these microbial communities, many species still have not been cultured in the lab. Environmental sequencing is the primary way the function and population dynamics of these communities can be studied. Long-read sequencing provides an avenue to overcome limitations of short-read technologies to obtain complete microbial genomes but comes with its own technical challenges, such as needed sequencing depth and obtaining high-quality DNA. We present here new sampling and bioinformatics methods to attempt decomposing an estuarine microbiome into its constituent genomes. Our results suggest there are only a few strains that comprise most of the species abundances from viruses to picoeukaryotes, and to fully decompose a metagenome of this diversity requires 1 Tbp of long-read sequencing. We anticipate that as long-read sequencing technologies continue to improve, less sequencing will be needed.

Lui, Lauren M.↗

Deep Realistic Extragalactic Model (DREaM) Galaxy Catalogs: Predictions for a Roman Ultra-deep Field

In the next decade, deep galaxy surveys from telescopes such as the James Webb Space Telescope and Roman Space Telescope will provide transformational data sets that will greatly enhance the understanding of galaxy formation during the epoch of reionization (EoR). In this work, we present the Deep Realistic Extragalactic Model (DREaM) for creating synthetic galaxy catalogs. Our model combines dark matter simulations, subhalo abundance matching and empirical models, and includes galaxy positions, morphologies, and spectral energy distributions. The resulting synthetic catalog extends to redshifts z ~ 12, and galaxy masses log 10 (M/M ⊙ ) = 5 covering an area of 1 deg 2 on the sky. We use DREaM to explore the science returns of a 1 deg 2 Roman ultra-deep field (UDF), and to provide a resource for optimizing ultra-deep survey designs. We find that a Roman UDF to ~30 m AB will potentially detect more than 10 6 M UV < -17 galaxies, with more than 10 4 at redshifts z > 7, offering an unparalleled data set for constraining galaxy properties during the EoR. Our synthetic catalogs and simulated images are made publicly available to provide the community with a tool to prepare for upcoming data.

79 ASTRONOMY AND ASTROPHYSICS↗

Exploring the Birth and Evolution of the Universe: How Detectors Have Revolutionized Space Astronomy

The past century has seen tremendous advances in the capability of instruments used for astronomical imaging and spectroscopy. Capabilities of instruments have expanded in many dimensions; the scale of telescopes has grown tremendously, the wavelengths used for astronomy have grown from visible light to the full electromagnetic spectrum, extending from gamma rays to low frequency radio waves. Additional advances have been enabled by the availability of space facilities, which eliminate the effects of the earths atmosphere and magnetosphere, and allow cooling of instruments to avoid instrumental thermal radiation. Even with all these advances, the increase in capability of detection systems has produced truly revolutionary improvements in capability. Today, I will describe the advances in astronomical detection from the photographic plates of the early 20th century to the giant high efficiency focal planes being developed for modern space and ground based astronomical instrument. I will review the demanding performance requirements set by space astronomy, and show how the detector community has risen to the challenge in producing high performance detectors for the Hubble Space Telescope, the Spitzer Space Telescope, and the James Webb Space Telescope, now under development.

Moseley, Samuel H.↗

The Prospective Search for Highly Ionized Technetium in Hot (pre-) White Dwarfs

The discovery of technetium (Tc) in the atmospheres of red giants by Merrill (1952, ApJ, 116, 21) constituted convincing proof that s-process nucleosynthesis is indeed occurring in evolved stars. In principle, Tc should still be present in the atmospheres of hot post-AGB stars and (pre-) white dwarfs although, due to radioactive decay, it should be present in decreasing quantities along post-AGB evolution. The recent discovery of a large number of trans-iron group elements in hot white dwarfs with atomic numbers in the range A = 30-56 (Zn to Ba) raises the prospect that Tc (A = 43) may also be detected. However, this is currently not feasible because no atomic data exist for ionization stages beyond Tc ii. As an initial step, we calculated atomic energy levels and oscillator strengths of Tc iv-vi and used these data to compute non-local thermodynamic equilibrium (NLTE) model atmospheres to estimate at which minimum abundance level Tc could be detected. We show that Tc lines can be found in ultraviolet spectra of hot white dwarfs provided Tc is as abundant as other detected trans-Fe elements. We find that radiative levitation can keep Tc in large, easily detectable quantities in the atmosphere. A direct identification of Tc lines is still not feasible because wavelength positions cannot be computed with necessary precision. Laboratory measurements are necessary to overcome this problem. Our results suggest that such efforts are beneficial to the astrophysical community.

prospective↗

NASA Disasters Program: A Partner for Coastal Disaster Response, Resiliency, and Risk Reduction

Summary: The NASA Applied Sciences: Disasters Program has kicked off a pilot project as “Mid-Atlantic Resiliency Demonstration Study, Communities at Intensive Risk” in FY17 and FY18; Efforts focus on a holistic, interdisciplinary approach to integrate a full suite of capabilities from numerical weather prediction, tide and surge modeling at high spatial resolution and urban/neighborhood scales, and remote sensing capabilities for water detection and depth estimation; Future efforts - Partner with Federal Agencies, academia professional societies to complement and augment ongoing work using Earth observations unique to NASA - Translate hazards to risk in order to understand local and regional impacts and minimize vulnerability.

Bell, Jordan↗

Initial Characterization and Optimization of the Liquid Sampling-Atmospheric Pressure Glow Discharge Ionization Source Coupled to an Orbitrap Mass Spectrometer for the Determination of Plutonium

Plutonium measurements are essential to the nuclear forensics and safeguards community. The liquid sampling-atmospheric pressure glow discharge (LS-APGD) microplasma ionization source coupled with an Orbitrap mass spectrometer is a proven platform for uranium isotope ratio determinations. Here, this work expands the LS-APGD-Orbitrap platform capabilities by reporting the first-ever analysis of plutonium with the LS-APGD and the first-ever measurement of elemental plutonium with an Orbitrap mass spectrometer. This coupling has the potential to dramatically reduce the complex sample manipulations required for traditional analysis techniques employed for actinide isotope ratio determinations. As a first step toward the goal of simultaneous uranium and plutonium isotope ratio determinations, the initial characterization and optimization of the platform for the detection of plutonium are reported. Collision-induced dissociation modality settings were optimized to reduce water-related and other molecular clusters containing plutonium, maximizing 242 Pu 16 O 2 + responses. A design of experiments study was conducted to optimize the discharge conditions of the dual-electrode LS-APGD toward the responsivity of 242 Pu 16 O 2 +. The measurement sensitivity was determined from a Pu response curve, yielding a limit of detection of 10 fg (absolute) of total analyte when data was collected and processed with a Spectroswiss FTMS Booster X2 data acquisition system. Additionally, plutonium and uranium were measured in a simultaneous acquisition, and each analyte remained unaffected by the other. It is believed that the LS-APGD-Orbitrap platform could be a valuable addition to the nuclear forensics’ toolbox and, indeed, other scientific disciplines and regulatory communities in which rapid, high-resolution plutonium determinations are paramount.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

In Situ Detection of Amino Acids from Bacterial Biofilms and Plant Root Exudates by Liquid Microjunction Surface-Sampling Probe Mass Spectrometry

The plant rhizosphere is a complex and dynamic chemical environment where the exchange of molecular signals between plants, microbes, and fungi drives the development of the entire biological system. Exogenous compounds in the rhizosphere are known to affect plant-microbe organization, interactions between organisms, and ultimately, growth and survivability. The function of exogenous compounds in the rhizosphere is still under much investigation, specifically with respect to their roles in plant growth and development, the assembly of the associated microbial community, and the spatiotemporal distribution of molecular components. A major challenge for spatiotemporal measurements is developing a nondisruptive and nondestructive technique capable of analyzing the exogenous compounds contained within the environment. A methodology using liquid microjunction-surface sampling probe-mass spectrometry (LMJ-SSP-MS) and microfluidic devices with attached microporous membranes was developed for in situ, spatiotemporal measurement of amino acids (AAs) from bacterial biofilms and plant roots. Exuded arginine was measured from a living Pantoea YR 343 biofilm, which resulted in a chemical image indicative of biofilm growth within the device. Spot sampling along the roots of Populus trichocarpa with the LMJ-SSP-MS resulted in the detection of 15 AAs. Of note, variation in AA concentrations across the root system was observed, indicating that exudation is not homogeneous and may be linked to local rhizosphere architecture and different biological processes along the root.

59 BASIC BIOLOGICAL SCIENCES↗

The Beam Dump eXperiment

Hadronic matter makes about 14% of the known universe. The remaining 86% is Dark Matter (DM). Since it does not interact with the ordinary matter via electromagnetic force, DM is not visible and, to date, it escaped detection. The search for Dark Matter (DM) is one of the hottest topic in modern physics. Despite the increasing number of astrophysical and cosmological observations proving the existence, so far no particle physics experiment has detected DM yet. Up to now, most of the experimental efforts have been focused on the so called WIMP (Weakly Interacting Massive Particle) paradigm, which predicts heavy DM (10 GeV-10 TeV mass range) interacting with Standard Model (SM) particles via the weak force mediators (W or Z bosons). More recently, due to the lack of clear evidence of WIMPs, other models of DM gained the interest of the physics community. These models consider Light DM particles (LDM), in MeV-GeV mass range. Among LDM theories, the Dark Photon theory predicts the existence of a Dark Sector interacting with SM particles via a new massive vector boson (Dark Photon, Heavy Photon or A0), mediator of a new force. This scenario, despite being theoretically well motivated, is remarkably experimentally unexplored. The Beam Dump eXperiment (BDX), is an approved experiment at Jefferson Lab (JLab), aiming to discover the DM predicted witihn the Dark Photon theory. The experiment uses the CEBAF (Continuous Electron Beam Accelerator Facility) 11 GeV electron beam, impinging on the JLab Hall-A beam-dump, to produce a beam of DM particles, detected by a ~ 1 m3 detector made of thallium doped cesium iodide (CsI(Tl)) crystals located ~ 20 m downstream. In order to achieve excellent background rejection, the experimental setup includes active vetos and passive shielding surrounding CsI(Tl) crystals. Given the weakness of the DM-SM interaction, the scattering of a DM particle in the BDX detector is a rare event. Therefore, the characterization of the expected background is a critical aspect of the experiment. Both cosmogenic and penetrating SM particles produced by the beam interaction in the dump contribute to the background of the experiment. While the cosmogenic contribution can be measured during the experiment when the beam is off, beam-related background can only be estimated via Monte Carlo (MC) simulations. Therefore, a careful assessment of possible systematics introduced by the MC is needed. The beam-correlated background characterization was made by measuring the muon flux produced by the interaction of the CEBAF beam with Hall-A dump in a dedicated experimental campaign in spring 2018 [1]. The comparison to the ?ux predicted by MC allowed us to validate the BDX simulation framework. The reach of BDX, i.e. the region that the experiment can probe in the LDM theory parameter space, depends critically on the background rejection capability and signal detection effciency. For this reason, the detector setup was fine-tuned through a dedicated study. The response to LDM and background events was evaluated for different setups and selection cuts. As a result of this procedure, the configuration resulting in the best sensitivity was selected [2]. The reach calculation was performed taking into account the different LDM production mechanisms, including the contribution of secondary particles produced in the beam-dump. In particular, the effect of the secondary positrons annihilation was found to be extremely significant. In the majority of the sensitivity studies, this contribution is neglected, but recent results [3] [4] demonstrated that this process significantly enhances the sensitivity of lepton beam-dump experiments. Currently, the BDX collaboration is focused on the deployment and operation of a small detector, called BDX-MINI, built to perform a preliminary physics measurement searching for LDM at JLab. This test will pave the way to the realization of the full BDX experiment. The measurement is currently ongoing but results are expected by the end of this year. During my PhD I was involved in all aspects of the BDX experiment: design, simulation, prototyping and data analysis. The main results of my work are reported in this thesis. This manuscript is organized as follows: the first Chapter provides an introduction to the theory of LDM, with particular attention to the Dark Photon paradigm; the second Chapter illustrates the BDX experimental setup, the LDM production and detection mechanisms and the expected backgrounds; Chapter 3 and 4 describe, respectively, the BDX-HODO measurement, with a detailed description of the simulations, and the BDX experimental setup and analysis cuts optimization. Chapter 5 reports about BDXMINI detector characterization, calibration and sensitivity estimate. Finally, Chapter 6 describes in detail the calculation of the secondary positron annihilation contribution to the sensitivity of BDX and other electron-beam thick-target experiment.

Marsicano, Luca↗

Detection Thresholds of Falling Snow from Satellite-Borne Active and Passive Sensors

Precipitation, including rain and snow, is a critical part of the Earth's energy and hydrology cycles. Precipitation impacts latent heating profiles locally while global circulation patterns distribute precipitation and energy from the equator to the poles. For the hydrological cycle, falling snow is a primary contributor in northern latitudes during the winter seasons. Falling snow is the source of snow pack accumulations that provide fresh water resources for many communities in the world. Furthermore, falling snow impacts society by causing transportation disruptions during severe snow events. In order to collect information on the complete global precipitation cycle, both liquid and frozen precipitation must be collected. The challenges of estimating falling snow from space still exist though progress is being made. These challenges include weak falling snow signatures with respect to background (surface, water vapor) signatures for passive sensors over land surfaces, unknowns about the spherical and non-spherical shapes of the snowflakes, their particle size distributions (PSDs) and how the assumptions about the unknowns impact observed brightness temperatures or radar reflectivities, differences in near surface snowfall and total column snow amounts, and limited ground truth to validate against. While these challenges remain, knowledge of their impact on expected retrieval results is an important key for understanding falling snow retrieval estimations. Since falling snow from space is the next precipitation measurement challenge from space, information must be determined in order to guide retrieval algorithm development for these current and future missions. This information includes thresholds of detection for various sensor channel configurations, snow event system characteristics, snowflake particle assumptions, and surface types. For example, can a lake effect snow system with low (approx 2.5 km) cloud tops having an ice water content (IWC) at the surface of 0.25 g / cubic m and dendrite snowflakes be detected? If this information is known, we can focus retrieval efforts on detectable storms and concentrate advances on achievable results. Here, the focus is to determine thresholds of detection for falling snow for various snow conditions over land and lake surfaces. The results rely on simulated Weather Research Forecasting (WRF) simulations of falling snow cases since simulations provide all the information to determine the measurements from space and the ground truth. Sensitivity analyses were performed to better ascertain the relationships between multifrequency microwave and millimeter-wave sensor observations and the falling snow/underlying field of view. In addition, thresholds of detection for various sensor channel configurations, snow event system characteristics, snowflake particle assumptions, and surface types were studied. Results will be presented for active radar at Ku, Ka, and W-band and for passive radiometer channels from 10 to 183 GHz.

Gail Skofronick-Jackson↗

A synthetic Roman Space Telescope High-Latitude Time-Domain Survey: supernovae in the deep field

ABSTRACT NASA will launch the Nancy Grace Roman Space Telescope (hereafter Roman) in the second half of this decade, which will allow for a generation-defining measurement of dark energy through multiple probes, including Type Ia supernovae (SNe Ia). To improve decisions on survey strategy, we have created the first simulations of realistic Roman images that include artificial SNe Ia injected as point sources in the images. Our analysis combines work done on Roman simulations for weak gravitational lensing studies as well as catalogue-level simulations of SN Ia samples. We have created a time series of images over 2 yr containing ∼1050 SNe Ia, covering a 1 deg2 subarea of a planned 5 deg2 deep survey. We have released these images publicly for community use along with input catalogues of all injected sources. We create secondary products from these images by generating coadded images and demonstrating recovery of transient sources using image subtraction. We perform first-use analyses on these images in order to measure galaxy detection efficiency, point source detection efficiency, and host-galaxy association biases. The simulated images can be found here at https://roman.ipac.caltech.edu/sims/SN_Survey_Image_sim.html.

Astronomy & Astrophysics↗

Listening for Neutrinos with Quantum Acoustics

Many low-threshold superconducting detectors rely on athermal phonon sensing, in which meV-scale phonons from a DM scatter are sensed via their ability to break Cooper pairs in superconducting films. While detectors based on such pairbreaking sensors are highly advantageous and scalable, their sensor threshold is ultimately limited by the energy needed to break a Cooper pair, 2Δ. We present a novel detector architecture for single phonon detection at the O(10μeV) scale, the qubit-coupled hBAR, that does not share this limitation. This architecture, composed of a superconducting qubit piezoelectrically coupled to a high-overtone bulk acoustic resonator, was originally developed in the context of the quantum acoustics community and has a sensor threshold ultimately limited by thermal noise. Such ultra-low-threshold detectors have a wide range of fundamental science applications, including reactor monitoring via neutrinos.

Baxter, Daniel Stephen [Fermilab] (ORCID:000000030↗

A Cloud Mask for AIRS

With the preliminary release of AIRS Level 1 and 2 data to the scientific community, there is a growing need for an accurate AIRS cloud mask for data assimilation studies and in producing products derived from cloud free radiances. Current cloud information provided with the AIRS data are limited or based on simplified threshold tests. A multispectral cloud detection approach has been developed for AIRS that utilizes the hyper-spectral capabilities to detect clouds based on specific cloud signatures across the short wave and long wave infrared window regions. This new AIRS cloud mask has been validated against the existing AIRS Level 2 cloud product and cloud information derived from MODIS. Preliminary results for both day and night applications over the continental U.S. are encouraging. Details of the cloud detection approach and validation results will be presented at the conference.

Brubaker, N.↗

A First Look at the DGEN380 Engine Acoustic Data from a Core-Noise Perspective

This work is a first look at acoustic data acquired in the NASA Glenn Research Center Aero-Acoustic Propulsion Laboratory using the Price Induction DGEN380 small turbofan engine, with particular emphasis on broadband combustor (core) noise. Combustor noise is detected by using a two-signal source separation technique employing one engine-internal sensor and one semi-far-field microphone. Combustor noise is an important core-noise component and is likely to become a more prominent contributor to overall airport community noise due to turbofan design trends, expected aircraft configuration changes, and advances in fan-noise-mitigation techniques. This work was carried out under the NASA Fundamental Aeronautics Program, Fixed Wing Project, Quiet Performance Subproject

Combustor Noise↗

PERISCOPE: PERIapsis Subsurface Cave OPtical Explorer

In this NIAC report we present a new technique enabling a practical option for mapping these structures at a relatively low cost: Photon Time-of-Flight (PTOF) imaging. Consider a scene with a surface that is in a camera's line of sight, with unknown geometry beyond the line of sight. PTOF works by directing laser pulses onto the visible surface and detecting the returned light after it reflects off the visible surface, onto the hidden surfaces, and back to the detector. An algorithm then reconstructs a 3d model of the hidden surface. The information gained has a wide variety of applications across NASA, the scientific community, the burgeoning commercial space industry, and in commercial and industrial use here on Earth.

Exploration↗

New York Ecological Forecasting: Utilizing NASA Earth Observations to Map Ash Distribution and Inform Emerald Ash Borer Control

Since their first sightings in the U.S. in 2002, emerald ash borer beetles (Agrilus planipennis; EAB) have killed millions of native ash (Fraxinus spp.) trees across 35 states. Infected ash stands frequently exhibit complete mortality, with the predicted result being the functional extinction of native ash in U.S. forests. In August of 2020, EAB was discovered in the 6.1-million-acre Adirondack Park. The team’s partners at the Adirondack Park Invasive Plant Program (APIPP) desired ash tree distribution and EAB susceptibility information to help improve EAB bio-control efficiency and apply the methodology to future invasive programs. To assist, the team mapped ash tree distribution using NASA Earth observations from Landsat 7 Enhanced Thematic Mapper Plus (ETM+) and Shuttle Radar Topography Mission (SRTM), along with hyperspectral imagery from the Airborne Visible/Infrared Imaging Spectrometer (AVIRIS). Field data from the Monitoring and Managing Ash (MaMA) project, iMapInvasives and iNaturalist databases, and the New York State Department of Environmental Conservation (NYSDEC) provided ground truthing for mapping and modeling. Results indicate that for ash detection, the team’s Spectral Angle Mapping (SAM) hyperspectral classification is slightly more sensitive but less accurate than multispectral Random Forest (RF) classification, though neither method was above a ~20% detection rate. End products include maps of ash extent derived from both imagery types, a model forecasting future spread scenarios based on current EAB presence, and outreach materials. These products inform APIPP’s management decisions and facilitate public awareness of EAB’s threat to communities within the region.

Liam Megraw↗

A 2-million-year-old ecosystem in Greenland uncovered by environmental DNA

Late Pliocene and Early Pleistocene epochs 3.6 to 0.8 million years ago had climates resembling those forecasted under future warming. Palaeoclimatic records show strong polar amplification with mean annual temperatures of 11–19 °C above contemporary values. The biological communities inhabiting the Arctic during this time remain poorly known because fossils are rare. Here we report an ancient environmental DNA (eDNA) record describing the rich plant and animal assemblages of the Kap København Formation in North Greenland, dated to around two million years ago. The record shows an open boreal forest ecosystem with mixed vegetation of poplar, birch and thuja trees, as well as a variety of Arctic and boreal shrubs and herbs, many of which had not previously been detected at the site from macrofossil and pollen records. The DNA record confirms the presence of hare and mitochondrial DNA from animals including mastodons, reindeer, rodents and geese, all ancestral to their present-day and late Pleistocene relatives. The presence of marine species including horseshoe crab and green algae support a warmer climate than today. The reconstructed ecosystem has no modern analogue. The survival of such ancient eDNA probably relates to its binding to mineral surfaces. Our findings open new areas of genetic research, demonstrating that it is possible to track the ecology and evolution of biological communities from two million years ago using ancient eDNA.

58 GEOSCIENCES↗

Long-Term field testing of the accuracy and HVAC energy savings potential of occupancy presence sensors in A Single-Family home

The energy-saving potential of occupancy-centric smart thermostats has been extensively explored in simulations but lacked field testing for energy savings quantification and sensor performance assessment in real buildings. This paper presents a long-term field study conducted in a single-family home in Texas, U.S. to evaluate the performance of occupancy-centric controls (OCC) of HVAC (heating, ventilation, and air-conditioning) system in terms of energy savings, sensor accuracy, and impact on electric peak demand. The test site was equipped with a commercial off-the-shelf (COTS) smart thermostat and multiple occupancy presence sensors for OCC implementation. Additionally, a sub-metering system was installed to monitor electricity consumption of various end-use equipment, including the HVAC system. A supplementary device was installed to track the ground-truth occupancy for the accuracy evaluation of the occupancy presence sensor. Scenarios of baseline and OCC controls were alternated weekly over the 20-month testing period. The results indicated an effective OCC execution, as evidenced by indoor temperature profiles. During the 2023 cooling season, OCC achieved total energy savings of 1,958 kWh, corresponding to a 17.6% energy savings ratio. Under certain conditions, daily HVAC energy savings reached as high as 17 kWh, with a savings ratio of 35%. Sensor performance showed an overall accuracy of 83.8%, a False Positive Rate (FPR) of 12.8%, and a False Negative Rate (FNR) of 47.4%. A key limitation was the sensor’s inability to detect stationary occupants during sleep, leading to a midnight FNR of nearly 100% and significantly compromising thermal comfort. Additionally, the implementation of OCC resulted in extended periods of high electricity demand on summer afternoons, affecting occupant’s thermal comfort and posing potential challenges to community-level grid operations if OCC were widely adopted. Furthermore, this study addresses a critical research gap by empirically investigating energy-saving potential and occupancy sensor performance in residential buildings. Through a comprehensive field-testing study, the research examines the interrelationship between sensor accuracy, energy savings, and thermal comfort, an area that has received limited attention in the current literature.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗