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At least 577 records · Page 32

Mach-Zehnder interferometry using spin- and valley-polarized quantum Hall edge states in graphene

Confined to a two-dimensional plane, electrons in a strong magnetic field travel along the edge in one-dimensional quantum Hall channels that are protected against backscattering. These channels can be used as solid-state analogs of monochromatic beams of light, providing a unique platform for studying electron interference. Electron interferometry is regarded as one of the most promising routes for studying fractional and non-Abelian statistics and quantum entanglement via two-particle interference. However, creating an edge-channel interferometer in which electron-electron interactions play an important role requires a clean system and long phase coherence lengths. We realize electronic Mach-Zehnder interferometers with record visibilities of up to 98% using spin- and valley-polarized edge channels that copropagate along a pn junction in graphene. We find that interchannel scattering between same-spin edge channels along the physical graphene edge can be used to form beamsplitters, whereas the absence of interchannel scattering along gate-defined interfaces can be used to form isolated interferometer arms. Surprisingly, our interferometer is robust to dephasing effects at energies an order of magnitude larger than those observed in pioneering experiments on GaAs/AlGaAs quantum wells. Lastly, our results shed light on the nature of edge-channel equilibration and open up new possibilities for studying exotic electron statistics and quantum phenomena.

36 MATERIALS SCIENCE↗

Improved High-Quality Draft Genome Sequence of the Eurypsychrophile Rhodotorula sp. JG1b, Isolated from Permafrost in the Hyperarid Upper-Elevation McMurdo Dry Valleys, Antarctica

Here, we report the draft genome sequence of Rhodotorula sp. strain JG1b, a yeast that was isolated from ice-cemented permafrost in the upper-elevation McMurdo Dry Valleys, Antarctica. The sequenced genome size is 19.39 Mb, consisting of 156 scaffolds and containing a total of 5,625 predicted genes. This is the first known cold-adapted Rhodotorula sp. sequenced to date.

54 ENVIRONMENTAL SCIENCES↗

A genome-wide RNA interference screen identifies a role for Wnt/β-catenin signaling during Rift Valley Fever Virus infection

Rift Valley fever virus (RVFV) is an arbovirus within the Bunyaviridae family capable of causing serious morbidity and mortality in humans and livestock. To identify host factors involved in bunyavirus replication, we employed genome-wide RNA interference (RNAi) screening and identified 381 genes whose knockdown reduced infection. The Wnt pathway was the most represented pathway when gene hits were functionally clustered. With further investigation, we found that RVFV infection activated Wnt signaling, was enhanced when Wnt signaling was preactivated, was reduced with knockdown of β-catenin, and was blocked using Wnt signaling inhibitors. Similar results were found using distantly related bunyaviruses La Crosse virus and California encephalitis virus, suggesting a conserved role for Wnt signaling in bunyaviral infection. We propose a model where bunyaviruses activate Wnt-responsive genes to regulate optimal cell cycle conditions needed to promote efficient viral replication. The findings in this study should aid in the design of efficacious host-directed antiviral therapeutics. IMPORTANCE RVFV is a mosquito-borne bunyavirus that is endemic to Africa but has demonstrated a capacity for emergence in new territories (e.g., the Arabian Peninsula). As a zoonotic pathogen that primarily affects livestock, RVFV can also cause lethal hemorrhagic fever and encephalitis in humans. Currently, there are no treatments or fully licensed vaccines for this virus. Using high-throughput RNAi screening, we identified canonical Wnt signaling as an important host pathway regulating RVFV infection. The beneficial role of Wnt signaling was observed for RVFV, along with other disparate bunyaviruses, indicating a conserved bunyaviral replication mechanism involving Wnt signaling. Lastly, these studies supplement our knowledge of the fundamental mechanisms of bunyavirus infection and provide new avenues for countermeasure development against pathogenic bunyaviruses.

59 BASIC BIOLOGICAL SCIENCES↗

Localization of Upper-Valley Electrons in a Narrow-Bandgap Channel: a Possible Additional Mechanism of Current Increase in DA–DpHEMT

The influence of the localization of upper-valley electrons in a narrow-bandgap channel on the drift velocity burst in Al{sub x}Ga{sub 1 –x}As–GaAs transistor heterostructures with double-sided doping has been theoretically estimated. It is established that the fraction of electrons passing in this case from the narrow-bandgap channel to wide-bandgap material is smaller than in the usual structures, which can lead in some cases to an increase in the electron drift velocity by up to 15%. This phenomenon can provide an additional mechanism of current increase in transistors based on heterostructures with donor–acceptor doping.

75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND↗

Temperature uncertainty modelling with proxy structural data as geostatistical constraints for well siting: an example applied to Granite Springs Valley, NV, USA

Utilizing existing temperature and structural geology information around Granite Springs Valley, Nevada, we build 3D stochastic temperature models with the aims of evaluating the 3D uncertainty of temperature and choosing between candidate exploration well locations. The data used to support the modelling are measured temperatures and structural proxies from 3D geologic modelling (distance to fault, distance to fault intersections and terminations, Coulomb stress change and dilation tendency), the latter considered ‘secondary’ data. Two stochastic geostatistical techniques are explored for incorporating the structural proxies: cosimulation and local varying mean. With both the cosimulation and local varying mean methods, many equally-likely temperature models (i.e. realizations) are produced, from which temperature probability profiles are calculated at candidate well locations. To aid in choosing between the candidate locations, two quantities summarize the temperature probabilities: V prior and entropy. V prior quantifies the likelihood for economic temperatures at each candidate location, whereas entropy identifies where new information has the most potential to reduce uncertainty. In general, the cosimulation realizations have smoother spatial structure, and extrapolate high temperatures at candidate locations that are located along the direction of the longest spatial correlation, which are down dip from existing temperature logs. The smooth realizations result in tight temperature probability profiles that are easier to interpret, but they have unrealistic temperature reversals in some locations because of the dipping ellipsoid shape created and that the cosimulation technique does not enforce a conductive geothermal gradient as a baseline (i.e. linearly increasing temperature with depth). The local varying mean results produce realizations with more realistic geothermal gradients, with temperatures increasing downward since a depth-temperature relationship is included. However, because they have much noisier spatial nature compared to cosimulation, it is harder to interpret the temperature probability profiles. The different local varying mean results allow the geologist to determine which proxy (e.g. dilation v. distance to fault termination) should be used given the specific geothermal system. In general, V prior from local varying mean results identify locations that are close to high values for the structural proxies: areas with higher probabilities for higher temperatures. The entropy results identify where uncertainty is greatest and therefore new drilling information could be most useful. Though these techniques provide useful information, even when applied to areas of sparse data, our comparison of these two techniques demonstrates the need for new geothermal geostatistics techniques that combine the advantages of these two methods and that are tailored to the spatial uncertainty issues inherent in geothermal exploration.

15 GEOTHERMAL ENERGY↗

Geysers Valley CO 2 Cycling Geological Engine (Kamchatka, Russia)

1941–2017 period of the Valley of Geysers monitoring (Kamchatka, Kronotsky Reserve) reveals a very dynamic geyser behavior under natural state conditions: significant changes of IBE (interval between eruptions) and power of eruptions, chloride and other chemical components, and preeruption bottom temperature. Nevertheless, the total deep thermal water discharge remains relatively stable; thus all of the changes are caused by redistribution of the thermal discharge due to giant landslide of June 3, 2007, mudflow of Jan. 3, 2014, and other events of geothermal caprock erosion and water injection into the geothermal reservoir. In some cases, water chemistry and isotope data point to local meteoric water influx into the geothermal reservoir and geysers conduits. TOUGHREACT V.3 modeling of Velikan geyser chemical history confirms 20% dilution of deep recharge water and CO 2 components after 2014. Temperature logging in geysers Velikan (1994, 2007, 2015, 2016, and 2017) and Bolshoy (2015, 2016, and 2017) conduits shows preeruption temperatures below boiling at corresponding hydrostatic pressure, which means partial pressure of CO 2 creates gas-lift upflow conditions in geyser conduits. Velikan geyser IBE history explained in terms of gradual CO 2 recharge decline (1941–2013), followed by CO 2 recharge significant dilution after the mudflow of Jan. 3, 2014, also reshaped geyser conduit and diminished its power.

58 GEOSCIENCES↗

Seroprevalence of Brucella spp. and Rift Valley fever virus among slaughterhouse workers in Isiolo County, northern Kenya

Brucella spp. and Rift Valley fever virus (RVFV) are classified as priority zoonotic agents in Kenya, based on their public health and socioeconomic impact on the country. Data on the pathogen-specific and co-exposure levels is scarce due to limited active surveillance. This study investigated seroprevalence and co-exposure of Brucella spp. and RVFV and associated risk factors among slaughterhouse workers in Isiolo County, northern Kenya. A cross-sectional serosurvey was done in all 19 slaughterhouses in Isiolo County, enrolling 378 participants into the study. The overall seroprevalences for Brucella spp. and RVFV were 40.2% (95% CI: 35.2–45.4) and 18.3% (95% CI: 14.5–22.5), respectively while 10.3% (95% CI 7.4%-13.8%) of individuals were positive for antibodies against both Brucella spp. and RVFV. Virus neutralisation tests (VNT) confirmed anti-RVFV antibodies in 85% of ELISA-positive samples. Our seroprevalence results were comparable to community-level seroprevalences previously reported in the area. Since most of the study participants were not from livestock-keeping households, our findings attribute most of the detected infections to occupational exposure. The high exposure levels indicate slaughterhouse workers are the most at-risk population and there is need for infection, prevention, and control programs among this high-risk group. This is the first VNT confirmation of virus-neutralising antibodies among slaughterhouse workers in Isiolo County and corroborates reports of the area being a high-risk RVFV area as occasioned by previously reported outbreaks. This necessitates sensitization campaigns to enhance awareness of the risks involved and appropriate mitigation measures.

60 APPLIED LIFE SCIENCES↗

Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase

Activated protein kinase R (PKR) plays a vital role in antiviral defense primarily by inhibiting protein synthesis and augmenting interferon responses. Many viral proteins have adopted unique strategies to counteract the deleterious effects of PKR. The NSs (Non-structural s) protein which is encoded by Rift Valley fever virus (RVFV) promotes early PKR proteasomal degradation through a previously undefined mechanism. In this study, we demonstrate that NSs carries out this activity by assembling the SCF (SKP1-CUL1-F-box)FBXW11 E3 ligase. NSs binds to the F-box protein, FBXW11, via the six amino acid sequence DDGFVE called the degron sequence and recruits PKR through an alternate binding site to the SCFFBXW11 E3 ligase. We further show that disrupting the assembly of the SCFFBXW11-NSs E3 ligase with MLN4924 (a small molecule inhibitor of SCF E3 ligase activity) or NSs degron viral mutants or siRNA knockdown of FBXW11 can block PKR degradation. Surprisingly, under these conditions when PKR degradation was blocked, NSs was essential and sufficient to activate PKR causing potent inhibition of RVFV infection by suppressing viral protein synthesis. These antiviral effects were antagonized by the loss of PKR expression or with a NSs deleted mutant virus. Therefore, early PKR activation by disassembly of SCFFBXW11-NSs E3 ligase is sufficient to inhibit RVFV infection. Furthermore, FBXW11 and BTRC are the two homologues of the βTrCP (Beta-transducin repeat containing protein) gene that were previously described to be functionally redundant. However, in RVFV infection, among the two homologues of βTrCP, FBXW11 plays a dominant role in PKR degradation and is the limiting factor in the assembly of the SCFFBXW11 complex. Thus, FBXW11 serves as a master regulator of RVFV infection by promoting PKR degradation. Overall these findings provide new insights into NSs regulation of PKR activity and offer potential opportunities for therapeutic intervention of RVFV infection.

59 BASIC BIOLOGICAL SCIENCES↗

WISE-CASING: Surface Seismic Survey at Cymric Field, California Central Valley

This test was conducted at the Chevron Cymric oilfield in the California central valley near Bakersfield. A reflected seismic signal was observed in all three components (x, y, z) of the 3-component Episensor geophone, as well as all phones on the single component array. The arrival time of the reflected seismic signal matches calculations based on a reasonable velocity model (~650 m/s). The seismic data has three channels that are from the 3-C Broadband Episensor, then from 4th -- 12th channels has no data. Channel 13 -- 25 are surface single change vertical geophones. The source of this seismic survey is weight drop. More info could be found from the data header and the attached PPT file.

15 GEOTHERMAL ENERGY↗

2002 Treasure Valley Transportation Survey

The 2002 Treasure Valley Transportation Survey was conducted in Ada and Canyon counties in southwest Idaho, under contract with the Community Planning Association of Southwest Idaho. The full study was conducted during September 2002 and October 2002 and entailed the collection of activity and travel information for all household members, regardless of age, during an assigned 24-hour period (Tuesday, Wednesday, or Thursday). In addition to providing basic demographic information about each household and its members, the survey documented specific travel characteristics and trips made, including the number of occupants, trip purpose, time of day, and questions specific to mode use. Travel days for the survey were spread across the pilot study (August 8, 2002) and the full study (September 3, 2002-October 31, 2002). In total, 3,488 households were recruited to participate in the study. Of these, 2,582 completed travel diaries (fully completed and passed edit check procedures), and the information was retrieved from all household members.

1Hz data↗

Shrub Expansion Simulations at Trail Valley Creek Tundra site using E3SM Land Model (ELM) Arctic-focused Version

The warming of the Arctic is causing substantial compositional, structural, and functional changes in tundra vegetation including shrub and densification in parts of the Arctic. Assessing the impact of these changes in vegetation composition on the Arctic’s carbon and energy budgets is important to constrain projected local and global surface-atmosphere exchanges. We conduct a sensitivity analysis of the projected surface energy fluxes, soil carbon pools, and carbon dioxide fluxes (net ecosystem exchange, gross primary production, and ecosystem respiration) between present day and 2100 to different shrub expansion rates and air temperature increases under future emission scenarios (intermediate – RCP4.5, and high – RCP8.5) using the Arctic-focused version of the Energy Exascale Earth System Model (E3SM) Land Model (ELM). We focus on Trail Valley Creek (TVC), a mineral upland tundra site located in the western Canadian Arctic, which is experiencing tall shrub densification and expansion. In this study, we run TVC under two different warming scenarios RCP4.5 and RCP 8.5 and simulate different shrubification rates projected until year 2100. In this repository, we include all the forcing, input, parameters, and output data corresponding to all the simulations performed. flmd.csv includes a detailed description of the datasets files.

54 ENVIRONMENTAL SCIENCES↗

AmeriFlux US-CaV Canaan Valley

This is the AmeriFlux version of the carbon flux data for the site US-CaV Canaan Valley. Site Description - None supplied.

Meyers, Tilden↗

AmeriFlux US-HVa Happy Valley

This is the AmeriFlux version of the carbon flux data for the site US-HVa Happy Valley. Site Description - Arctic tundra:tossock tundra

Oechel, Walt↗

AmeriFlux CA-TVC Trail Valley Creek

This is the AmeriFlux version of the carbon flux data for the site CA-TVC Trail Valley Creek. Site Description - Southern Arctic tundra site, erect-shrub tundra, tundra dominated by low shrubs <40 cm, continuous permafrost

Sonnentag, Oliver↗

Properties of Noise Cross-Correlation Functions Obtained from a Distributed Acoustic Sensing Array at Garner Valley, California

A field test that was conducted at Garner Valley, California, on 11 and 12 September 2013 using distributed acoustic sensing (DAS) to sense ground vibrations provided a continuous overnight record of ambient noise. Furthermore, the energy of ambient noise was concentrated between 5 and 25 Hz, which falls into the typical traffic noise frequency band. A standard procedure (Bensen et al., 2007) was adopted to calculate noise cross-correlation functions (NCFs) for 1-min intervals. The 1-min-long NCFs were stacked using the time–frequency domain phase-weighted-stacking method, which significantly improves signal quality. The obtained NCFs were asymmetrical, which was a result of the nonuniform distributed noise sources. A precursor appeared on NCFs along one segment, which was traced to a strong localized noise source or a scatterer at a nearby road intersection. NCF for the radial component of two surface accelerometers along a DAS profile gave similar results to those from DAS channels. Here, we calculated the phase velocity dispersion from DAS NCFs using the multichannel analysis of surface waves technique, and the result agrees with active-source results. We then conclude that ambient noise sources and the high spatial sampling of DAS can provide the same subsurface information as traditional active-source methods.

58 GEOSCIENCES↗