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Search indexed NASA NTRS and DOE OSTI research on propulsion, heat transfer, battery materials and energy systems. Follow report and document links to the original sources.

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At least 577 records · Page 32

Calmodulin activation of an endoplasmic reticulum-located calcium pump involves an interaction with the N-terminal autoinhibitory domain

To investigate how calmodulin regulates a unique subfamily of Ca(2+) pumps found in plants, we examined the kinetic properties of isoform ACA2 identified in Arabidopsis. A recombinant ACA2 was expressed in a yeast K616 mutant deficient in two endogenous Ca(2+) pumps. Orthovanadate-sensitive (45)Ca(2+) transport into vesicles isolated from transformants demonstrated that ACA2 is a Ca(2+) pump. Ca(2+) pumping by the full-length protein (ACA2-1) was 4- to 10-fold lower than that of the N-terminal truncated ACA2-2 (Delta2-80), indicating that the N-terminal domain normally acts to inhibit the pump. An inhibitory sequence (IC(50) = 4 microM) was localized to a region within valine-20 to leucine-44, because a peptide corresponding to this sequence lowered the V(max) and increased the K(m) for Ca(2+) of the constitutively active ACA2-2 to values comparable to the full-length pump. The peptide also blocked the activity (IC(50) = 7 microM) of a Ca(2+) pump (AtECA1) belonging to a second family of Ca(2+) pumps. This inhibitory sequence appears to overlap with a calmodulin-binding site in ACA2, previously mapped between aspartate-19 and arginine-36 (J.F. Harper, B. Hong, I. Hwang, H.Q. Guo, R. Stoddard, J.F. Huang, M.G. Palmgren, H. Sze inverted question mark1998 J Biol Chem 273: 1099-1106). These results support a model in which the pump is kept "unactivated" by an intramolecular interaction between an autoinhibitory sequence located between residues 20 and 44 and a site in the Ca(2+) pump core that is highly conserved between different Ca(2+) pump families. Results further support a model in which activation occurs as a result of Ca(2+)-induced binding of calmodulin to a site overlapping or immediately adjacent to the autoinhibitory sequence.

NASA Program Fundamental Space Biology↗

Hypergravity exposure decreases gamma-aminobutyric acid immunoreactivity in axon terminals contacting pyramidal cells in the rat somatosensory cortex: a quantitative immunocytochemical image analysis

Quantitative evaluation of gamma-aminobutyric acid immunoreactivity (GABA-IR) in the hindlimb representation of the rat somatosensory cortex after 14 days of exposure to hypergravity (hyper-G) was conducted by using computer-assisted image processing. The area of GABA-IR axosomatic terminals apposed to pyramidal cells of cortical layer V was reduced in rats exposed to hyper-G compared with control rats, which were exposed either to rotation alone or to vivarium conditions. Based on previous immunocytochemical and behavioral studies, we suggest that this reduction is due to changes in sensory feedback information from muscle receptors. Consequently, priorities for muscle recruitment are altered at the cortical level, and a new pattern of muscle activity is thus generated. It is proposed that the reduction observed in GABA-IR of the terminal area around pyramidal neurons is the immunocytochemical expression of changes in the activity of GABAergic cells that participate in reprogramming motor outputs to achieve effective movement control in response to alterations in the afferent information.

Non-NASA Center↗

Identification of the in vivo truncation sites at the C-terminal region of alpha-A crystallin from aged bovine and human lens

Total alpha-A crystallin was purified from young versus old lens, followed by digestion with cyanogen bromide. Laser desorption mass spectrometry of the C-terminal fragment demonstrated age-dependent loss of one and five amino acids from the C-terminus of alpha-A crystallin from both bovine and human lens. These results demonstrate specific peptide bonds of alpha-A crystallin are cleaved during the aging process of the normal lens. The C-terminal region is cleaved in two places between the two hydroxyl-containing amino acids present in the sequence -P-S(T)-S-.

Non-NASA Center↗

Oxidation of the N-terminal methionine of lens alpha-A crystallin

Antiserum against the N-terminal peptide of bovine alpha-A crystallin has been used to monitor purification of two different seropositive peptides (i.e. T1a and T1b) from a tryptic digest of bovine lens proteins. Both these peptides have similar amino acid compositions, but peptide T1b has a molecular weight 16 atomic mass units larger than T1a, suggesting posttranslational modification. Analysis of ionization fragments of the T1b peptide by mass spectrometry demonstrates that this difference in molecular weight is due to the in vivo oxidation of the N-terminal met residue of the alpha-A crystallin molecule.

NASA Discipline Cell Biology↗

Simulating Virtual Terminal Area Weather Data Bases for Use in the Wake Vortex Avoidance System (Wake VAS) Prediction Algorithm

During the research project, sounding datasets were generated for the region surrounding 9 major airports, including Dallas, TX, Boston, MA, New York, NY, Chicago, IL, St. Louis, MO, Atlanta, GA, Miami, FL, San Francico, CA, and Los Angeles, CA. The numerical simulation of winter and summer environments during which no instrument flight rule impact was occurring at these 9 terminals was performed using the most contemporary version of the Terminal Area PBL Prediction System (TAPPS) model nested from 36 km to 6 km to 1 km horizontal resolution and very detailed vertical resolution in the planetary boundary layer. The soundings from the 1 km model were archived at 30 minute time intervals for a 24 hour period and the vertical dependent variables as well as derived quantities, i.e., 3-dimensional wind components, temperatures, pressures, mixing ratios, turbulence kinetic energy and eddy dissipation rates were then interpolated to 5 m vertical resolution up to 1000 m elevation above ground level. After partial validation against field experiment datasets for Dallas as well as larger scale and much coarser resolution observations at the other 8 airports, these sounding datasets were sent to NASA for use in the Virtual Air Space and Modeling program. The application of these datasets being to determine representative airport weather environments to diagnose the response of simulated wake vortices to realistic atmospheric environments. These virtual datasets are based on large scale observed atmospheric initial conditions that are dynamically interpolated in space and time. The 1 km nested-grid simulated datasets providing a very coarse and highly smoothed representation of airport environment meteorological conditions. Details concerning the airport surface forcing are virtually absent from these simulated datasets although the observed background atmospheric processes have been compared to the simulated fields and the fields were found to accurately replicate the flows surrounding the airport where coarse verification data were available as well as where airport scale datasets were available.

Kaplan, Michael L.↗

Statistical and Prediction modeling of the Ka Band Using Experimental Results from ACTS Propagation Terminals at 20.185 and 27.505 GHZ

With the increase in demand for wireless communication services, most of the operating frequency bands have become very congested. The increase of wireless costumers is only fractional contribution to this phenomenon. The demand for more services such as video streams and internet explorer which require a lot of band width has been a more significant contributor to the congestion in a communication system. One way to increase the amount of information or data per unit of time transmitted with in a wireless communication system is to use a higher radio frequency. However in spite the advantage available in the using higher frequency bands such as, the Ka-band, higher frequencies also implies short wavelengths. And shorter wavelengths are more susceptible to rain attenuation. Until the Advanced Communication Technology Satellite (ACTS) was launched, the Ka- band frequency was virtually unused - the majority of communication satellites operated in lower frequency bands called the C- and Ku- bands. Ka-band is desirable because its higher frequency allows wide bandwidth applications, smaller spacecraft and ground terminal components, and stronger signal strength. Since the Ka-band is a high frequency band, the millimeter wavelengths of the signals are easily degraded by rain. This problem known as rain fade or rain attenuation The Advanced Communication Technology Satellite (ACTS) propagation experiment has collected 5 years of Radio Frequency (RF) attenuation data from December 1993 to November 1997. The objective of my summer work is to help develop the statistics and prediction techniques that will help to better characterize the Ka Frequency band. The statistical analysis consists of seasonal and cumulative five-year attenuation statistics for the 20.2 and 27.5 GHz. The cumulative five-year results give the link outage that occurs for a given link margin. The experiment has seven ground station terminals that can be attributed to a unique rain zone climate. The locations are White Sands, NM, Tampa, Fly Clarksburg, MD, Norman, OK, Ft. Collins, COY Vancouver, BC, and Fairbanks, AK. The analysis will help us to develop and define specific parameters that will help system engineers develop the appropriated instrumentation and structure for a Ka-band wireless communication systems and networks.

Ogunwuyi, Oluwatosin O.↗

Mobile Phone Terminal

In the photo, an employee of a real estate firm is contacting his office by means of HICOM, an advanced central terminal for mobile telephones. Developed by the Orlando Division of Martin Marietta Aerospace, Orlando, Florida, and manufactured by Harris Corporation's RF Division, Rochester, N.Y., HICOM upgrades service to users, provides better system management to telephone companies, and makes more efficient use of available mobile telephone channels through a computerized central control terminal. The real estate man, for example, was able to dial his office and he could also have direct-dialed a long distance number. Mobile phones in most areas not yet served by HICOM require an operator's assistance for both local and long distance calls. HICOM improves system management by automatically recording information on all calls for accurate billing, running continual performance checks on its own operation, and reporting any malfunctions to a central office.

Source record↗

Wire Crimp Termination Verification Using Ultrasonic Inspection

The development of a new ultrasonic measurement technique to quantitatively assess wire crimp terminations is discussed. The amplitude change of a compressional ultrasonic wave propagating through the junction of a crimp termination and wire is shown to correlate with the results of a destructive pull test, which is a standard for assessing crimp wire junction quality. Various crimp junction pathologies such as undercrimping, missing wire strands, incomplete wire insertion, partial insulation removal, and incorrect wire gauge are ultrasonically tested, and their results are correlated with pull tests. Results show that the nondestructive ultrasonic measurement technique consistently (as evidenced with destructive testing) predicts good crimps when ultrasonic transmission is above a certain threshold amplitude level. A physics-based model, solved by finite element analysis, describes the compressional ultrasonic wave propagation through the junction during the crimping process. This model is in agreement within 6% of the ultrasonic measurements. A prototype instrument for applying this technique while wire crimps are installed is also presented. The instrument is based on a two-jaw type crimp tool suitable for butt-splice type connections. Finally, an approach for application to multipin indenter type crimps will be discussed.

Perey, Daniel F.↗

Reliability Block Diagram (RBD) Analysis of NASA Dryden Flight Research Center (DFRC) Flight Termination System and Power Supply

In order to perform public risk analyses for vehicles containing Flight Termination Systems (FTS), it is necessary for the analyst to know the reliability of each of the components of the FTS. These systems are typically divided into two segments; a transmitter system and associated equipment, typically in a ground station or on a support aircraft, and a receiver system and associated equipment on the target vehicle. This analysis attempts to analyze the reliability of the NASA DFRC flight termination system ground transmitter segment for use in the larger risk analysis and to compare the results against two established Department of Defense availability standards for such equipment.

Morehouse, Dennis V.↗

Initial Concept for Terminal Area Conflict Detection, Alerting, and Resolution Capability on or Near the Airport Surface

The Next Generation Air Transportation System (NextGen) concept for 2025 envisions the movement of large numbers of people and goods in a safe, efficient, and reliable manner. The NextGen will remove many of the constraints in the current air transportation system, support a wider range of operations, and deliver an overall system capacity up to 3 times that of current operating levels. In order to achieve the NextGen vision, research is necessary in the areas of surface traffic optimization, maximum runway capacity, reduced runway occupancy time, simultaneous single runway operations, and terminal area conflict prevention, among others. The National Aeronautics and Space Administration (NASA) is conducting Collision Avoidance for Airport Traffic (CAAT) research to develop technologies, data, and guidelines to enable Conflict Detection and Resolution (CD&R) in the Airport Terminal Maneuvering Area (ATMA) under current and emerging NextGen operating concepts. In this report, an initial concept for an aircraft-based method for CD&R in the ATMA is presented. This method is based upon previous NASA work in CD&R for runway incursion prevention, the Runway Incursion Prevention System (RIPS). CAAT research is conducted jointly under NASA's Airspace Systems Program, Airportal Project and the Aviation Safety Program, Integrated Intelligent Flight Deck Project.

Green, David F.↗

Effectiveness of Shield Termination Techniques Tested with TEM Cell and Bulk Current Injection

This paper presents experimental results of the effectiveness of various shield termination techniques. Each termination technique is evaluated by two independent noise injection methods; transverse electromagnetic (TEM) cell operated from 3 MHz 400 MHz, and bulk current injection (BCI) operated from 50 kHz 400 MHz. Both single carrier and broadband injection tests were investigated. Recommendations as to how to achieve the best shield transfer impedance (i.e. reduced coupled noise) are made based on the empirical data. Finally, the noise injection techniques themselves are indirectly evaluated by comparing the results obtained from the TEM Cell to those from BCI.

Bradley, Arthur T.↗

Use of Data Comm by Flight Crew in High-Density Terminal Areas

This paper describes a collaborative FAA and NASA experiment using 22 commercial airline pilots to determine the effect of using Datalink Communication (Data Comm) to issue messages in busy, terminal area operations. Four conditions were defined that span current day to future flight deck equipage levels (voice communication only, Data Comm only, Data Comm with Moving Map Display, Data Comm with Moving Map displaying taxi route), and each condition was used to create an arrival and a departure scenario at the Boston Logan Airport. These eight scenarios were repeated twice for a total of 16 scenarios for each of the eleven crews. Quantitative data was collected on subject reaction time and eye tracking information. Questionnaires collected subjective feedback on workload and acceptability to the flight crew for using Data Comm in a busy terminal area. 95% of the Data Comm messages were responded to by the flight crew within one minute; however, post experiment debrief comments revealed almost unanimous consensus that two minutes was a reasonable expectation for crew response. Eye tracking data indicated an insignificant decrease in head-up time for the Pilot Flying when Data Comm was introduced; however, the Pilot Monitoring had significantly less head-up time. Data Comm workload was rated as operationally acceptable by both crew members in all conditions in flight at any altitude above the Final Approach Fix in terms of response time and workload. Results also indicate the use of Data Comm during surface operations was acceptable, the exception being the simultaneous use of voice, Data Comm, and audio chime required for an aircraft to cross an active runway. Many crews reported they believed Data Comm messages would be acceptable after the Final Approach Fix or to cross a runway if the message was not accompanied by a chime and there was not a requirement to immediately respond to the uplink message.

Baxley, Brian T.↗

A Concept for Robust, High Density Terminal Air Traffic Operations

This paper describes a concept for future high-density, terminal air traffic operations that has been developed by interpreting the Joint Planning and Development Office s vision for the Next Generation (NextGen) Air Transportation System and coupling it with emergent NASA and other technologies and procedures during the NextGen timeframe. The concept described in this paper includes five core capabilities: 1) Extended Terminal Area Routing, 2) Precision Scheduling Along Routes, 3) Merging and Spacing, 4) Tactical Separation, and 5) Off-Nominal Recovery. Gradual changes are introduced to the National Airspace System (NAS) by phased enhancements to the core capabilities in the form of increased levels of automation and decision support as well as targeted task delegation. NASA will be evaluating these conceptual technological enhancements in a series of human-in-the-loop simulations and will accelerate development of the most promising capabilities in cooperation with the FAA through the Efficient Flows Into Congested Airspace Research Transition Team.

Isaacson, Douglas R.↗

A Simulation Based Approach to Optimize Berth Throughput Under Uncertainty at Marine Container Terminals

Berth scheduling is a critical function at marine container terminals and determining the best berth schedule depends on several factors including the type and function of the port, size of the port, location, nearby competition, and type of contractual agreement between the terminal and the carriers. In this paper we formulate the berth scheduling problem as a bi-objective mixed-integer problem with the objective to maximize customer satisfaction and reliability of the berth schedule under the assumption that vessel handling times are stochastic parameters following a discrete and known probability distribution. A combination of an exact algorithm, a Genetic Algorithms based heuristic and a simulation post-Pareto analysis is proposed as the solution approach to the resulting problem. Based on a number of experiments it is concluded that the proposed berth scheduling policy outperforms the berth scheduling policy where reliability is not considered.

Golias, Mihalis M.↗

Advanced Command Destruct System (ACDS) Enhanced Flight Termination System (EFTS)

This presentation provides information on the development, integration, and operational usage of the Enhanced Flight Termination System (EFTS) at NASA Dryden Flight Research Center and Air Force Flight Test Center. The presentation will describe the efforts completed to certify the system and acquire approval for operational usage, the efforts to integrate the system into the NASA Dryden existing flight termination infrastructure, and the operational support of aircraft with EFTS at Edwards AFB.

Tow, David K.↗

The Solar Wind Termination Shock and the Outer Heliosphere

Beginning in mid-2002, Voyager 1 observed an extended period of field-aligned flows of low energy ions and electrons that was unlike previous observations, suggesting that the spacecraft was in the vicinity of the solar wind termination shock. However, the observations differed from expectations and have resulted in differing interpretations as to whether or not Voyager 1 crossed the termination shock and entered the subsonic flow in the heliosheath for a seven month period. The puzzling aspects of the observations suggest that the shock is more complex and dynamic than generally expected and that there could be more surprises as Voyager 1 will likely encounter the shock several times before durably entering the heliosheath sometime in the next few years.

Stone, E. C.↗

Deep-Space Optical Terminals (DOT)

A conceptual design study titled Deep-Space Optical Terminals was recently completed for an optical communication technology demonstration from Mars in the 2018 time frame. We report on engineering trades for the entire system, and for individual subsystems including the flight terminal, the ground receiver and the ground transmitter. A point design is described to meet the requirement for greater than 0.25 Gb/s downlink from the nearest distance to Mars of 0.42 AU with a maximum mass and power allocation of 40 kg and 110 W. Furthermore, the concept design addresses link closure at the farthest Mars range of 2.7 AU. Maximum uplink data-rate of 0.3 Mb/s and ranging with 30 cm precision are also addressed.

optical communications↗