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At least 577 records · Page 32

Baseline Fuel Fabrication Facility

PRO-RR is the research reactor focused program element of the broader Proliferation Resistance Optimization program (PRO-X) under the National Nuclear Safety Administration (NNSA) in the U.S. Department of Energy (DOE). PRO-X provides a framework for integrating proliferation resistance in nuclear system designs to minimize weapons usable nuclear materials (WUNM) production and diversion pathways while optimizing systems performance for peaceful use missions. PRO-RR applies the PRO-X mission objectives to research reactor system design. This document serves as one of the foundational documents for the PRO-RR-Fuel System Design technical team by documenting a baseline fuel fabrication facility to be used for further optimization studies. The PRO-RR-Fuel System Design technical team consists of subject matter experts from Argonne National Laboratory (Argonne) and Savannah River National Laboratory (SRNL). In order to develop specific strategies for fuel fabrication facilities to optimize proliferation resistance, performance, and safety, a baseline fuel fabrication facility design basis was developed. Having a baseline design basis allows for the qualitative and quantitative comparison of design choices in the optimization process. This report describes the baseline fuel fabrication facility and general optimization strategy. Chapter 2 describes the fuel system selected for examination, the fabrication process used as the baseline, a description of the model developed to track uranium utilization, and a generic floorplan of the fabrication facility. Chapter 3 describes the overarching optimization strategy that could be implemented for a fabrication facility.

21 SPECIFIC NUCLEAR REACTORS AND ASSOCIATED PLANTS↗

Construction Methodology Transformation for the Benefit of Workforce Development

Construction is a key economic engine driving both national and global economies. While manual, onsite construction methods dominate the U.S. construction industry, a major shift towards offsite methods has been underway due to its efficiency, speed, and potential cost savings. The workforce necessary for offsite construction growth does not exist in its current form because of the focus on onsite methodologies and the lack of exposure to offsite building methods at all levels of a student’s learning journey. The growth of the U.S. construction industry and competitiveness in an increasingly global construction market over the coming decades can only be supported by a dramatic increase in the use of offsite methods, which requires ramping up workforce training for certain skillsets. The goal of Construction Methodology Transformation for the Benefit of Workforce Development was to understand the opportunities and barriers in both education and industry and to identify best practices for offering curriculum and training to educators, industry, and students that would support skills needed for careers in offsite construction. Our team proposed combining three offsite construction workforce development needs: content development, exposure and training, and job placement - under a single Platform model that would increase experience and career opportunities for students and help match them with potential industry members. Through our proposed solution we expected to see: developed offsite curriculum being utilized by educators and students; an increase in the identification of construction technology and offsite construction methods; an average increase in knowledge gain of at least 25% after participation in pilots; better equipped candidates who are prepared for jobs in offsite construction; and a beta workforce development platform that helps build more pathways for students looking for careers in offsite construction. The two pilots included almost 250 students and resulted in an average knowledge gain of 37 percent. Our research has identified areas of opportunity, for both education and industry to make collaborative training programs more efficient and successful. The chosen techniques for this program are extremely effective when both the school and factory have solid processes and cultures in place to accept students into training programs. This project serves as an important stepping stone to industrywide collaboration to move workforce development for offsite construction forward across the country. With continued collaboration programs like this can provide much needed early exposure and training in offsite construction and we can begin to fill important positions for the future of construction.

99 GENERAL AND MISCELLANEOUS↗

Arabidopsis DNA polymerase lambda mutant is mildly sensitive to DNA double strand breaks but defective in integration of a transgene

The DNA double-strand break (DSB) is a critical type of damage, and can be induced by both endogenous sources (e.g., errors of oxidative metabolism, transposable elements, programmed meiotic breaks, or perturbation of the DNA replication fork) and exogenous sources (e.g., ionizing radiation or radiomimetic chemicals). Although higher plants, like mammals, are thought to preferentially repair DSBs via nonhomologous end joining (NHEJ), much remains unclear about plant DSB repair pathways. Our reverse genetic approach suggests that DNA polymerase λ is involved in DSB repair in Arabidopsis . The Arabidopsis T-DNA insertion mutant (atpolλ-1) displayed sensitivity to both gamma-irradiation and treatment with radiomimetic reagents, but not to other DNA damaging treatments. The (atpolλ-1) mutant showed a moderate sensitivity to DSBs, while Arabidopsis Ku70 and DNA ligase 4 mutants (atku70-3 and atlig4-2) , both of which play critical roles in NHEJ, exhibited a hypersensitivity to these treatments. The atpolλ-1/atlig4-2 double mutant exhibited a higher sensitivity to DSBs than each single mutant, but the atku70/atpolλ-1 showed similar sensitivity to the atku70-3 . We showed that transcription of the DNA ligase 1, DNA ligase 6 , and Wee1 genes was quickly induced by BLM in several NHEJ deficient mutants in contrast to wild-type. Finally, the T-DNA transformation efficiency dropped in NHEJ deficient mutants and the lowest transformation efficiency was scored in the atpolλ-1/atlig4-2 double mutant. These results imply that AtPolλ is involved in both DSB repair and DNA damage response pathway.

Plant Sciences↗

Biofuels Refining Engineering

The goal of this project is the development of novel catalysts and knowledge of reaction pathways and mechanisms for conversion of biomass-based compounds to fuels that are compatible with oil-based fuels and with acceptable or superior fuel properties. The research scope included both catalysts to convert lignocellulosic biomass-based molecules (from pyrolysis) and vegetable oil-based molecules (i.e., triglycerides and fatty acid methyl esters). This project comprised five technical tasks. Each task is briefly introduced below, and major technical accomplishments summarized. Technical accomplishments were described in greater detail in the quarterly progress reports, and in even more detail in the >50 publications acknowledging this DoE project funding (list of publications and presentations included at the end of this report). The results of this research added greatly to the knowledge base necessary for upgrading of pyrolysis oil to hydrocarbon fuels and chemicals, and for conversion of vegetable oils to fungible diesel fuel. Numerous new catalysts and catalytic reaction systems were developed for upgrading particular compounds or compound families found in the biomass-based pyrolysis oils and vegetable oils. Methods to mitigate catalyst deactivation were investigated, including novel reaction/separation systems. Performance and emission characteristics of biofuels in flames and engines were measured. Importantly, the knowledge developed from this project became the basis for a subsequent collaborative proposal led by our research group, involving researchers from the University of Wisconsin, the University of Pittsburg, and the Idaho National Lab, for the DoE Carbon, Hydrogen and Separations Efficiency (CHASE) program, which was subsequently funded (one of only four projects awarded in the CHASE program). The CHASE project examined novel catalytic processes for lignocellulosic biomass conversion as well as technoeconomic analyses for process options for maximum carbon capture and hydrogen efficiency. Our research approach combined catalyst synthesis, measurements of catalyst activity and selectivity in different reactor systems and conditions, and detailed catalyst characterization to develop fundamental understanding of reaction pathways and the capability to predict product distributions. Nearly all of the candidate catalysts were prepared in-house via standard techniques such as impregnation, co-impregnation, or chemical vapor deposition. Supports were usually purchased, but in some cases coprecipitation was used to simultaneously create the support and active component, which can be advantageous for strong active component-support interactions and for achieving high active component dispersion. In-house synthesis also allowed for studies of the effects on catalyst activity and selectivity of such factors as support porosity, calcination temperature, and reduction/activation conditions. Depending on the physical characteristics of the molecule, catalyst activity measurements were carried out in tubular flow reactors (for vapor phase reactions) or stirred tank reactors (for liquid phase reactions) over a wide range of pressures and temperatures. Reactant and product concentrations were measured using gas chromatography (both on-line and off-line, with TCD, FID, and/or mass spectrometric detection). For promising catalysts, detailed physicochemical characterization was carried out using FTIR, Raman, XPS, and XRD spectroscopies (all available in our laboratories) and TEM spectroscopy (available at OU). Additional methods included temperature programmed techniques (TPD, TPO) and surface area measurements by nitrogen adsorption techniques.

01 COAL, LIGNITE, AND PEAT↗

Chloroplast Stress Signals: Control of Retrograde Signaling, Chloroplast Turn-Over, and Cell Fate Decisions

Chloroplasts (photosynthetic plastids) are semiautonomous organelles that contain their own small genomes. The proteomes of chloroplasts, however, are a mixture of plastid and nuclear-encoded proteins. Chloroplasts perform photosynthesis, which is prone to damaging the organelles, leading to the production of reactive oxygen species (ROS) that damage the cell under environmental stresses. Thus, for the cell to maintain proper chloroplast function, efficient photosynthesis, and avoid ROS damage, it relies on complex crosstalk between the chloroplast, the nucleus, other organelles within the cell, and the cytoplasm in between. This communication involves retrograde signals from chloroplasts to control nuclear gene expression, programmed cell death (PCD), and chloroplast degradation. Here we review these signals with an emphasis on the roles of the ROS singlet oxygen ( 1 O 2 ) and plastid gene expression. We cover (1) recent work on understanding how multiple 1 O 2 signaling pathways can be initiated within stressed chloroplasts, (2) how individualized post-translational regulatory systems allow chloroplasts to control their proteomes and degradation, and (3) how chloroplast signals ultimately control cell fate decisions, such as PCD, senescence, and vacuole-mediated degradation of chloroplasts (chloroplast quality control). Overall, this chapter discusses how chloroplasts can act as environmental sensors for the cell and allow plants to acclimate to stress and thrive in dynamic environments.

59 BASIC BIOLOGICAL SCIENCES↗

Programmed cell clearance: From nematodes to humans

Programmed cell clearance is a highly regulated physiological process of elimination of dying cells that occurs rapidly and efficiently in healthy organisms. It thus ensures proper development as well as homeostasis. Recent studies have disclosed a considerable degree of conservation of cell clearance pathways between nematodes and higher organisms. The externalization of the anionic phospholipid phosphatidylserine (PS) has emerged as an important “eat-me” signal for phagocytes and its exposition on apoptotic cells is controlled by phospholipid translocases and scramblases. However, there is mounting evidence that PS exposure occurs not only in apoptosis, but may also be actively expressed on the surface of cells undergoing other forms of cell death including necrosis; PS is also expressed on the surface of engulfing cells. Additionally, PS may act as a “save-me” signal during axonal regeneration. Here we discuss mechanisms of PS exposure and its recognition by phagocytes as well as the consequences of PS signaling in nematodes and in mammals. - Highlights: • Cell clearance pathways are conserved between nematodes and mammals. • Phosphatidylserine (PS) externalization is a common signal for cell clearance. • Translocases and scramblases are involved in PS exposure during apoptosis. • PS also functions as a “save-me” signal to mediate the fusion of injured axons. • Definitions of cell death should take into account whether/how cells are cleared.

60 APPLIED LIFE SCIENCES↗

Integrating Energy-Efficient Computing with Computational Research to Accelerate Energy Technology

NREL's computational sciences center hosts the largest high performance computing (HPC) capabilities dedicated to energy research while functioning as a living laboratory for energy-efficient computing. NREL's HPC capabilities support the research needs of the Department of Energy's Office of Energy Efficiency and Renewable Energy (EERE). In ten years of operation, HPC use in EERE-sponsored research has grown by a factor of 30, including work in electricity generation, energy efficiency, transportation, and energy system modeling. This paper analyzes this research portfolio, providing examples of individual use cases. The paper documents NREL's history of operating one of the world's most energy-efficient data centers while examining pathways to reduce economic and environmental impact beyond reduction of Power Usage Efficiency (PUE). This paper concludes by examining the unique opportunities created for accelerating improvements in data center efficiency created by combining an HPC system dedicated to energy research and a research program in energy-efficient computing.

97 MATHEMATICS AND COMPUTING↗

Energy-efficient Public Procurement: Best Practice in Program Delivery

This document illustrates the key issues and considerations involved in implementing energy-efficient public procurement. Our primary sources of information have been our partners in the Super Efficient Equipment and Appliance Deployment (SEAD) Initiative Procurement Working Group. Where applicable, we have highlighted specific ways in which working group participants have successfully overcome barriers to delivering effective programs. The following key points emerge from this analysis of programs for energy-efficient public procurement. Lessons for both developed and developing programs are highlighted throughout the guide. 1. Policy: Policy provides the initiative to begin a transition from first cost to life-cycle cost based purchasing methods and culture. Effective policy is well-communicated, establishes accountability from top to bottom of organizations and simplifies the processes necessary to comply. Flexibility and responsiveness are essential in policy development and implementation. Mandatory and voluntary policies may complement one another. 2. Procurement Criteria: Procurement staff must be confident that energy-efficient procurement criteria offer the best long-term value for their organization’s money and represent real environmental gains. Involving multiple stakeholders at the early stages of the criteria creation process can result in greater levels of cooperation from private industry. Criteria should make comparison of products easy for purchasers and require minimal additional calculations. Criteria will need to be regularly updated to reflect market developments. 3. Training: Resources for the creation of training programs are usually very limited, but well-targeted training is necessary in order for a program to be effective. Training must emphasize a process that is efficient for purchasers and simplifies compliance. Purchaser resources and policy must be well designed for training to be effective. Training program development is an excellent opportunity for collaboration amongst public authorities. 4. Procurement Processes: Many tools and guides intended to help buyers comply with energy-efficient procurement policy are designed without detailed knowledge of the procurement process. A deeper understanding of purchasing pathways allows resources to be better directed. Current research by national and international bodies aims to analyze purchasing pathways and can assist in developing future resources.

25 ENERGY STORAGE↗

Brazing Dissimilar Metals with a Novel Composite Foil

Reactive brazing is a novel technique in the joining industry that uses reactive materials to produce substantial amounts of heat locally to join components. In this project, we created mechanically processed dilute thermite foils (Redox Foils) that upon ignition selfpropagate to produce molten metal capable of joining dissimilar metal combinations. Due to the transient nature of the reaction, there is limited thermal damage to the base materials. Also, novel component combinations, that cannot be joined via tradition furnace brazing (such as magnesium alloys to steel), can be produced. Redox Foils are produced by mechanically processing and consolidating constituent powders. By adjusting the fabrication pathway and chemistry, the reaction products can be tailored to join specific material combinations. In addition to producing mechanically processed foils, we produce vapor processed foils with controlled microstructures to understand some of the mechanisms of the reaction. Over the course of the program, we worked to identify and enhance the properties of the Redox Foils so that they could produce strong braze bonds of dissimilar metals (steel, Al, and Mg alloys). In a first step, the reactivity of the powders, from which the foils were made, was enhanced by ball milling Al and metal oxide powders before consolidation. In a second step, Redox Foils were made by physical vapor deposition to identify what level of dilution is needed to prevent the formation of Cu vapor during the reaction of the Redox Foils. In a third step, we incorporated diluent powders within the milling process to improve homogeneity within the final foils. In addition, finite element model simulations of heat transfer were also combined with detailed microstructure studies to identify what level of homogeneity is needed to avoid the generation of Cu vapor. Lastly, we include Ag powders as a diluent to form a lower melting temperature braze. Unfortunately, none of these steps led to Redox Foils that could produce bonds with shear strengths greater than 10 MPa. The generation of oxygen vapor appears to be generating porous bond lines that are still relatively week. To circumvent this vapor generation, we investigated Ti:2B reactive scaffolds that provide a heated framework to enhance braze flow and minimize porosity. However, BM Ti:2B powders suffered ~8% mass loss due to surface adsorbed water resulting in porous and weak bonds. As an alternative solution, we developed Ti-C-Cu based reactive braze foils that rely on TiC formation reactions to melt the Cu braze material. In this system, the foils did not produce gas or vapor upon reaction but did form the desired TiC product with molten Cu. Unfortunately, the reaction temperatures and the duration of Cu melting were insufficient to produce effective bonds. The goal of achieving bond strengths of 20 MPa or higher was not achieved.

36 MATERIALS SCIENCE↗

Performance and Cost of a Natural Gas Fuel Cell Plant with Complete Internal Reforming

The performance and cost of a natural gas fueled, fuel cell (NGFC) power system with complete internal reformation (IR) is presented in this report. The report presents an update to a previous NGFC Pathway Study that includes carbon capture and storage (2011). IR utilizes heat generated in the fuel cell stack directly for the endothermic reformation reaction, improving system efficiency. NGFC systems with complete IR form the ultimate embodiment of the current fuel cell technology development program at the National Energy Technology Laboratory, which is consistent with the commercialization strategies being pursued in the stationary power generation sector.

03 NATURAL GAS↗

Cyclotron Road at Berkeley Lab – U.S. Department of Energy

The Department of Energy is testing a new model for clean energy research and development (R&D) through a program called Cyclotron Road. The goal is to support scientific R&D that is still too risky for private‐sector investment, and too applied for academia. Participants receive the time, space and capital to pursue their research and the support to find viable pathways to the market.

29 ENERGY PLANNING, POLICY, AND ECONOMY↗

Acetic Acid Reforming over Rh Supported on La2O3/CeO2-ZrO2: Catalytic Performance and Reaction Pathway Analysis

Reforming of acetic acid was investigated on Rh supported on CeO2-ZrO2 modified with 3 wt % La. The active catalyst converted acetic acid to H-2-rich gas and hardly formed coke. The low rate of coke formation is concluded to be related to the presence of redox-active oxygen limiting the concentration of coke precursors. Temperature-programmed O-18(2)) isotope exchange measurements showed that the La2O3 and Rh enhanced the mobility of lattice oxygen compared with that of the parent CeO2-ZrO2. Ketonization and decarboxylation of acetic acid are the dominating reactions over the latter up to 600 degrees C, whereas above 600 degrees C, steam reforming and water gas shift also contribute. Over 0.5 wt % Rh on La2O3/CeO2-ZrO2, reforming and water gas shift reactions dominate, even below 300 degrees C, producing mostly H-2 and CO2. Using isotope labeling, it is shown that acetic acid adsorbs dissociatively on Rh, forming acetates, which sequentially decarboxylate and form surface methyl groups. The latter are in turn converted to CO, CO2, and H-2.

acetic acid↗

Radiological Safety Analysis Computer Program V 7.2

Users generates a fission product inventory from either reactor operating history or a nuclear criticality event. RSAC-7 models the effects of high-efficiency particulate air filters or other cleanup systems and calculates the decay and ingrowth during transport through processes, facilities, and the environment. Doses are calculated for inhalation, air immersion, ground surface, ingestion, and cloud gamma pathways. RSAC-7 is used as a tool to evaluate accident conditions in emergency response scenarios, radiological sabotage events, and safety basis accident consequences.

Jordan, RobertaA. [Idaho National Laboratory (INL)↗

Silencing of Hsp27 and Hsp72 in glioma cells as a tool for programmed cell death induction upon temozolomide and quercetin treatment

The aim of the present study was to investigate whether silencing of Hsp27 or Hsp72 expression in glioblastoma multiforme T98G and anaplastic astrocytoma MOGGCCM cells increases their sensitivity to programmed cell death induction upon temozolomide and/or quercetin treatment. Transfection with specific siRNA was performed for the Hsp gene silencing. As revealed by microscopic observation and flow cytometry, the inhibition of Hsp expression was correlated with severe apoptosis induction upon the drug treatment studied. No signs of autophagy were detected. This was correlated with a decreased mitochondrial membrane potential, increased level of cytochrome c in the cytoplasm, and activation of caspase 3 and caspase 9. All these results suggest that the apoptotic signal was mediated by an internal pathway. Additionally, in a large percentage of cells treated with temozolomide, with or without quercetin, granules within the ER system were found, which was accompanied by an increased level of caspase 12 expression. This might be correlated with ER stress. Quercetin and temozolomide also changed the shape of nuclei from circular to “croissant like” in both transfected cell lines. Our results indicate that blocking of Hsp27 and Hsp72 expression makes T98G cells and MOGGCCM cells extremely vulnerable to apoptosis induction upon temozolomide and quercetin treatment and that programmed cell death is initiated by an internal signal. - Highlights: • Hsps gene silencing induced severe apoptosis upon temozolomide–quercetin treatment • Apoptosis in transfected glioma cells was initiated by internal signal • Programmed cell death was preceded by ER stress • Temozolomide–quercetin treatment changed nuclei shape in transfected glioma cells.

60 APPLIED LIFE SCIENCES↗

Mycotoxins: A Fungal Genomics Perspective

The chemical and enzymatic diversity in the fungal kingdom is staggering. Large-scale fungal genome sequencing projects are generating a massive catalog of secondary metabolite biosynthetic genes and pathways. Fungal natural products are a boon and bane to man as valuable pharmaceuticals and harmful toxins. Understanding how these chemicals are synthesized will aid the development of new strategies to limit mycotoxin contamination of food and feeds as well as expand drug discovery programs. A survey of work focused on the fumonisin family of mycotoxins highlights technological advances and provides a blueprint for future studies of other fungal natural products. Expressed sequence tags led to the discovery of new fumonisin genes (FUM) and hinted at a role for alternatively spliced transcripts in regulation. Phylogenetic studies of FUM genes uncovered a complex evolutionary history of the FUM cluster, as well as fungi with the potential to synthesize fumonisin or fumonisin-like chemicals. The application of new technologies (e.g., CRISPR) could substantially impact future efforts to harness fungal resources.

Brown, Daren W.↗

Post-Transcriptional Mechanisms Respond Rapidly to Ecologically Relevant Thermal Fluctuations During Temperature-Dependent Sex Determination

An organism’s ability to integrate transient environmental cues experienced during development into molecular and physiological responses forms the basis for adaptive shifts in phenotypic trajectories. During temperature-dependent sex determination (TSD), thermal cues during discrete periods in development coordinate molecular changes that ultimately dictate sexual fate and contribute to patterns of inter- and intra-sexual variation. How these mechanisms interface with dynamic thermal environments in nature remain largely unknown. By deploying thermal loggers in wild nests of the American alligator (Alligator mississippiensis) over two consecutive breeding seasons, we observed that 80% of nests exhibit both male- and female-promoting thermal cues during the thermosensitive period, and of these nests, all exhibited both male- and female-promoting temperatures within the span of a single day. These observations raise a critical question—how are opposing environmental cues integrated into sexually dimorphic transcriptional programs across short temporal scales? To address this question, alligator embryos were exposed to fluctuating temperatures based on nest thermal profiles and sampled over the course of a daily thermal fluctuation. We examined the expression dynamics of upstream genes in the temperature-sensing pathway and find that post-transcriptional alternative splicing and transcript abundance of epigenetic modifier genes JARID2 and KDM6B respond rapidly to thermal fluctuations while transcriptional changes of downstream effector genes, SOX9 and DMRT1, occur on a delayed timescale. Our findings reveal how the basic mechanisms of TSD operate in an ecologically relevant context. We present a hypothetical hierarchical model based on our findings as well as previous studies, in which temperature-sensitive alternative splicing incrementally influences the epigenetic landscape to affect the transcriptional activity of key sex-determining genes.

59 BASIC BIOLOGICAL SCIENCES↗

WE-G-204-00: Post-Graduate Training of the Next Generation of Academic Medical Physicists

While many indicators for academic medical physics are distressing – jobs are tight, demands on clinical time are high (and getting worse) and national funding has been flat for several years (meaning less money in reality) the present is perhaps one of the most exciting times in cancer research history, and medical physicists have an opportunity to make a difference. Many of us predict the impact of medical physics on cancer research over the next decade to be more significant than ever. Why is that? First, medical imaging is used for every cancer patient in developed countries. Every improvement in the acquisition, processing or analysis of radiological images has the potential to impact patients. The use of radiation therapy is at an all-time high – and virtually cannot be performed without medical physics. Many of the advances in both biomedical imaging and radiation oncology are the result of the hard work of academic medical physicists who are thinking of the next generation of technologies that will be used against cancer or an even broader spectrum of diseases. A career in academic medical physics is demanding, particularly for those with clinical responsibilities. As the demands for justification of their clinical effort become increasingly metricized, the ability to do “unfunded research” will become even more difficult. This means that many will have to generate external salary support to justify their efforts in research and development. This comes at a time when funding for research is compressed and harder to obtain. Generally speaking, if you are not contributing 50% or more of your effort to research, you are competing at a disadvantage and it is very unlikely you will get an NIH/NCI/NIBIB grant. Furthermore, in the ongoing effort to improve patient care and safety, we have developed credentialing pathways that now require at least two-years of residency training. This full-time clinical training creates a gap in the research trajectory of graduate students who aspire to academic positions with an expectation for extramural funding. To address this, several residency programs have created hybrid programs where the two-years of clinical training is combined with one or two years of research effort to allow candidates to further establish an academic identity and to ensure adequate academic productivity to compete for a beginning faculty position. In conclusion, while the path to a successful career in academic medical physics is steep and sometimes hard to follow, reaching the apex is worth the journey. Different paths to a career in medical physics are available, you just have to decide which one is right for you. If improving cancer care is your goal as a physicist, then academic medical physics is the job for you!.

60 APPLIED LIFE SCIENCES↗