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At least 577 records · Page 32

Intrinsic and extrinsic factors interact during development to influence telomere length in a long‐lived reptile

Abstract The mechanisms connecting environmental conditions to plasticity in biological aging trajectories are fundamental to understanding individual variation in functional traits and life history. Recent findings suggest that telomere biology is especially dynamic during early life stages and has long‐term consequences for subsequent reproduction and survival. However, our current understanding is mostly derived from studies investigating ecological and anthropogenic factors separately, leaving the effects of complex environmental interactions unresolved. American alligators ( Alligator mississippiensis ) are long‐lived apex predators that rely on incubation temperature during a discrete period during development and endocrine cues to determine sex, making them especially vulnerable to current climatic variability and exposure to anthropogenic contaminants interfering with hormone function. Here, we combine field studies with a factorial design to understand how the developmental environment, along with intrinsic biological variation contribute to persistent telomere variation. We found that exposure to a common endocrine disrupting contaminant, DDE, affects telomere length, but that the directionality is highly dependent upon incubation temperature. Variation in hatchling growth, underlies a strong clutch effect. We also assess concentrations of a panel of glucocorticoid hormones and find that contaminant exposure elicits an increase in circulating glucocorticoids. Consistent with emerging evidence linking stress and aging trajectories, GC levels also appear to trend with shorter telomere length. Thus, we add support for a mechanistic link between contaminants and glucocorticoid signalling, which interacts with ecological aspects of the developmental environment to alter telomere dynamics.

Bae, Junsoo↗

Complex interactions between day length and diurnal patterns of gene expression drive photoperiodic responses in a perennial C 4 grass

Abstract Photoperiod is a key environmental cue affecting flowering and biomass traits in plants. Key components of the photoperiodic flowering pathway have been identified in many species, but surprisingly few studies have globally examined the diurnal rhythm of gene expression with changes in day length. Using a cost‐effective 3′‐Tag RNA sequencing strategy, we characterize 9,010 photoperiod responsive genes with strict statistical testing across a diurnal time series in the C 4 perennial grass, Panicum hallii . We show that the vast majority of photoperiod responses are driven by complex interactions between day length and sampling periods. A fine‐scale contrast analysis at each sampling time revealed a detailed picture of the temporal reprogramming of cis ‐regulatory elements and biological processes under short‐ and long‐day conditions. Phase shift analysis reveals quantitative variation among genes with photoperiod‐dependent diurnal patterns. In addition, we identify three photoperiod enriched transcription factor families with key genes involved in photoperiod flowering regulatory networks. Finally, coexpression networks analysis of GIGANTEA homolog predicted 1,668 potential coincidence partners, including five well‐known GI‐interacting proteins. Our results not only provide a resource for understanding the mechanisms of photoperiod regulation in perennial grasses but also lay a foundation to increase biomass yield in biofuel crops.

Weng, Xiaoyu↗

High-Pressure Laminar Flame Speeds and Markstein Lengths of Syngas Flames Diluted in Carbon Dioxide and Helium

Abstract New laminar flame speed and burned-gas Markstein length data for H2–CO–O2–CO2–He mixtures have been measured from spherically expanding flames. Experiments were conducted at 10 atm and room temperature for H2:CO ratios ranging from 2:1 to 1:4 and for overall CO2 mole fractions from 0% to 30%. CO2 dilution had little effect on Markstein length, but CO2 dilutions of 10%, 20%, and 30% caused average reductions in flame speed of 47%, 73%, and 89%, respectively, regardless of H2:CO ratio. The study was designed to isolate the dilution effect of CO2 on flame speed, and a detailed analysis using the FCO2 method was used to show that the chemical-kinetic participation of CO2 was responsible for up to 20% of the reduction in flame speed. Hence, the majority (80% or more) of the reduction in flame speed due to CO2 is from the thermal effect. Accurate flame speed predictions were produced by five different chemical kinetics mechanisms for most conditions, with the slight exception of high-CO, high-CO2 mixtures. A thorough sensitivity analysis highlighted the larger effect of CO2 dilution on the important kinetics reactions than the effect of changing H2:CO. Sensitivity analysis also showed that the chain branching reaction H2O + O ⇌ OH + OH could be modified (albeit beyond its uncertainty) to achieve more accurate flame speed predictions, but also indicated that further improvement of flame speed modeling would require changes to many lesser reactions.

Engineering↗

SU-E-T-554: Monte Carlo Calculation of Source Terms and Attenuation Lengths for Neutrons Produced by 50–200 MeV Protons On Brass

Purpose: We used TOPAS (TOPAS wraps and extends Geant4 for medical physicists) to compare Geant4 physics models with published data for neutron shielding calculations. Subsequently, we calculated the source terms and attenuation lengths (shielding data) of the total ambient dose equivalent (TADE) in concrete for neutrons produced by protons in brass. Methods: Stage1: The Bertini and Binary nuclear models available in Geant4 were compared with published attenuation at depth of the TADE in concrete and iron. Stage2: Shielding data of the TADE in concrete was calculated for 50– 200 MeV proton beams on brass. Stage3: Shielding data from Stage2 was extrapolated for 235 MeV proton beams. This data was used in a point-line-source analytical model to calculate the ambient dose per unit therapeutic dose at two locations inside one treatment room at the Francis H Burr Proton Therapy Center. Finally, we compared these results with experimental data and full TOPAS simulations. Results: At larger angles (∼130o) the TADE in concrete calculated with the Bertini model was about 9 times larger than that calculated with the Binary model. The attenuation length in concrete calculated with the Binary model agreed with published data within 7%±0.4% (statistical uncertainty) for the deepest regions and 5%±0.1% for shallower regions. For iron the agreement was within 3%±0.1%. The ambient dose per therapeutic dose calculated with the Binary model, relative to the experimental data, was a ratio of 0.93±0.16 and 1.23±0.24 for two locations. The analytical model overestimated the dose by four orders of magnitude. These differences are attributed to the complexity of the geometry. Conclusion: The Binary and Bertini models gave comparable results, with the Binary model giving the best agreement with published data at large angle. Shielding data we calculated using the Binary model is useful for fast shielding calculations with other analytical models. This work was supported by National Cancer Institute Grant R01CA140735.

61 RADIATION PROTECTION AND DOSIMETRY↗

Recording interfacial currents on the subnanometer length and femtosecond time scale by terahertz emission

Electron dynamics at interfaces is a subject of great scientific interest and technological importance. Detailed understanding of such dynamics requires access to the angstrom length scale defining interfaces and the femtosecond time scale characterizing interfacial motion of electrons. In this context, the most precise and general way to remotely measure charge dynamics is through the transient current flow and the associated electromagnetic radiation. Here, we present quantitative measurements of interfacial currents on the subnanometer length and femtosecond time scale by recording the emitted terahertz radiation following ultrafast laser excitation. We apply this method to interlayer charge transfer in heterostructures of two transition metal dichalcogenide monolayers less than 0.7 nm apart. We find that charge relaxation and separation occur in less than 100 fs. This approach allows us to unambiguously determine the direction of current flow, to demonstrate a charge transfer efficiency of order unity, and to characterize saturation effects.

36 MATERIALS SCIENCE↗

Leakless end-to-end transport of small molecules through micron-length DNA nanochannels

Designed and engineered protein and DNA nanopores can be used to sense and characterize single molecules and control transmembrane transport of molecular species. However, designed biomolecular pores are less than 100 nm in length and are used primarily for transport across lipid membranes. Nanochannels that span longer distances could be used as conduits for molecules between nonadjacent compartments or cells. We design micrometer-long, 7-nm-diameter DNA nanochannels that small molecules can traverse according to the laws of continuum diffusion. Binding DNA origami caps to channel ends eliminates transport and demonstrates that molecules diffuse from one channel end to the other rather than permeating through channel walls. These micrometer-length nanochannels can also grow, form interconnects, and interface with living cells. This work thus shows how to construct multifunctional, dynamic agents that control molecular transport, opening ways of studying intercellular signaling and modulating molecular transport between synthetic and living cells.

59 BASIC BIOLOGICAL SCIENCES↗

Bond-length dependence of attosecond ionization delays in O 2 arising from electron correlation to a shape resonance

We experimentally and theoretically demonstrate that electron correlation can cause the bond-length sensitivity of a shape resonance to induce an unexpected vibrational state–dependent ionization delay in a nonresonant channel. This discovery was enabled by a high-resolution attosecond-interferometry experiment based on a 400-nm driving and dressing wavelength. The short-wavelength driver results in a 6.2–electron volt separation between harmonics, markedly reducing the spectral overlap in the measured interferogram. We demonstrate the promise of this method on O 2 , a system characterized by broad vibrational progressions and a dense photoelectron spectrum. We measure a 40-attosecond variation of the photoionization delays over the X 2 Π g vibrational progression. Multichannel calculations show that this variation originates from a strong bond-length dependence of the energetic position of a shape resonance in the b 4 Σ$^{-}_{g}$ channel, which translates to the observed effects through electron correlation. The unprecedented energy resolution and delay accuracies demonstrate the promise of visible-light–driven molecular attosecond interferometry.

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CH↗

Effect of the length of ligands passivating quantum dots on the electrooptical characteristics of organic light-emitting diodes

The electrooptical characteristics of organic light-emitting diodes with quantum dots passivated with organic ligands of different lengths as emitting centers are investigated. It is established that the thickness of the ligand coating covering the quantum dots has little effect on the Förster energy transfer in the diodes, but significantly affects the direct injection of charge carriers into the quantum-dot layer. It is shown that the thickness of the passivation coating covering the quantum dots in a close-packed nanoparticle layer is deter- mined both by the length of passivating ligands and the degree of quantum-dot coverage with ligands.

36 MATERIALS SCIENCE↗

A Finite Length Cylinder Model for Mixed Oxide-Ion and Electron Conducting Cathodes Suited for Intermediate-Temperature Solid Oxide Fuel Cells

A physics-based model is presented to simulate the electrochemical behavior of mixed ion and electron conducting (MIEC) cathodes for intermediate-temperature solid oxide fuel cells. Analytic solutions for both transient and impedance models based on a finite length cylinder are derived. These solutions are compared to their infinite length counterparts. The impedance solution is also compared to experimental electrochemical impedance spectroscopy data obtained from both a traditional well-established La 0.6 Sr 0.4 Co 0.2 Fe 0.8 O 3-δ (LSCF) cathode and a new SrCo 0.9 Nb 0.1 O 3-δ (SCN) porous cathode. Lastly, the impedance simulations agree well with the experimental values, demonstrating that the new models can be used to extract electro-kinetic parameters of MIEC SOFC cathodes.

25 ENERGY STORAGE↗

Single-shot optical recorder with sub-picosecond resolution and scalable record length on a semiconductor wafer

In this study, we demonstrate a novel, single-shot recording technology for transient optical signals. A resolution of 0.4 ps over a record length of 54 ps was demonstrated. Here, a pump pulse crossing through a signal samples a diagonal “slice” of space–time, enabling a camera to record spatially the time content of the signal. Unlike related X (2) -based cross-correlation techniques, here the signal is sampled through optically pumped carriers that modify the refractive index of a silicon wafer. Surrounding the wafer with birefringent retarders enables two time-staggered, orthogonally polarized signal copies to probe the wafer. Recombining the copies at a final crossed polarizer destructively interferes with them, except during the brief stagger window, where a differential phase shift is incurred. This enables the integrating response of the rapidly excited but persistent carriers to be optically differentiated. Lastly, this sampling mechanism has several advantages that enable scaling to long record lengths, including making use of large, inexpensive semiconductor wafers, eliminating the need for phase matching, broad insensitivity to the spectral and angular properties of the pump, and overall hardware simplicity.

42 ENGINEERING↗

Cell-length heterogeneity: a population-level solution to growth/virulence trade-offs in the plant pathogen Dickeya dadantii

Necrotrophic plant pathogens acquire nutrients from dead plant cells, which requires the disintegration of the plant cell wall and tissue structures by the pathogen. Infected plants lose tissue integrity and functional immunity as a result, exposing the nutrient rich, decayed tissues to the environment. One challenge for the necrotrophs to successfully cause secondary infection (infection spread from an initially infected plant to the nearby uninfected plants) is to effectively utilize nutrients released from hosts towards building up a large population before other saprophytes come. In this study, we observed that the necrotrophic pathogen Dickeya dadantii exhibited heterogeneity in bacterial cell length in an isogenic population during infection of potato tuber. While some cells were regular rod-shape (<10μm), the rest elongated into filamentous cells (>10μm). Short cells tended to occur at the interface of healthy and diseased tissues, during the early stage of infection when active attacking and killing is occurring, while filamentous cells tended to form at a later stage of infection. Short cells expressed all necessary virulence factors and motility, whereas filamentous cells did not engage in virulence, were non-mobile and more sensitive to environmental stress. However, compared to the short cells, the filamentous cells displayed upregulated metabolic genes and increased growth, which may benefit the pathogens to build up a large population necessary for the secondary infection. The segregation of the two subpopulations was dependent on differential production of the alarmone guanosine tetraphosphate (ppGpp). When exposed to fresh tuber tissues or freestanding water, filamentous cells quickly transformed to short virulent cells. The pathogen adaptation of cell length heterogeneity identified in this study presents a model for how some necrotrophs balance virulence and vegetative growth to maximize fitness during infection.

54 ENVIRONMENTAL SCIENCES↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗

2003 Interstate 595 Vehicle Trip-Length Study

# 2003 Interstate 595 Vehicle Trip-Length Study The 2003 Vehicle Trip-Length Study focused on Interstate 595 between Davie Road and University Drive in Florida. Survey participants answered questions about their trip's origin and destination—including the type of location such as work, home, store, etc.—the on- and off-ramps used, how many people were in the car, and the type of vehicle. The survey also collected household demographic data such as annual household income, available vehicles, the number of people living in their household, and the number of workers in their household above the age of 16. It also asked if they would use proposed bus-only lanes or train service along the corridor, if available. ## Data Collection Agency The survey was conducted by and for the Florida Department of Transportation. ## Survey Methodology The survey was conducted via mail and online in March 2003. ## Survey Records, Data, and Documentation Survey records include 7,917 participants. Origin and destination locations include street addresses, nearest intersections or landmarks, city, state, zip code, and latitude/longitude.

32 ENERGY CONSERVATION, CONSUMPTION, AND UTILIZATI↗