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At least 577 records · Page 32

Adaptation of rat soleus muscles to 4 wk of intermittent strain

The effect of repeated strains on rat soleus muscles was investigated by stretching active muscles 3 times/wk for 4 wk with two different methods of stretching. The adaptation of myofibers and noncontractile tissue was followed by histochemical techniques and computer-assisted image analysis. Muscle hypertrophy was seen in the slow-stretched muscles, which increased in mass by 13% and increased in myofiber cross-sectional area by 30%. In the fast-stretched muscle, mass increased by 10% but myofiber cross-sectional area actually decreased. This decrease in mean fiber area was the result of a population of very small fibers (population A) that coexisted with slightly smaller normal-sized fibers (population B). Fibers in population A did not have the distribution expected from atrophy compared with atrophic fibers from unloaded muscles; they were much smaller. In addition, there was a 44% increase in noncontractile tissue in the fast-stretched muscles. Thus, soleus muscles subjected to repeated strains respond differently to slow and fast stretching. Slow stretching results in typical muscle hypertrophy, whereas fast stretching produces somewhat larger muscles but with a mixture of small and normal-sized myofibers accompanied by a marked proliferation of noncontractile tissue.

Stauber, W. T.↗

Magnetic Or Optical Surface Layer Would Indicate Strain

In proposed method of obtaining information on strain at surface of material specimen, magnetic coat (like that on magnetic tape) or optical coat (like that on compact disk) applied to all or part of surface monitored. Coating layer and associated measuring equipment, taken together, constitute system called "material strain monitor" (MSM). MSM important in research in materials and mechanics; in particular, expected to compete strongly with systems based on image-analysis and laser techniques now being developed to obtain information on strain fields.

Heyman, Joseph S.↗

Effects of energy release on near field flow structure of gas jets

The primary objective is to understand how buoyancy affects the structure of the shear layer, the development of fluid dynamic instabilities, and formation of the coherent structures in the near-nozzle regions of gas jets. The secondary objectives are to study the role of buoyancy in lifting and reattachment process of diffusion flames, to evaluate the scaling behavior of diffusion flames, and to aid development and/or validation of theoretical models by providing quantitative data in the absence of buoyancy. Fast reacting hydrogen or hydrogen-inert fuels are used to isolate the effects of buoyancy on fluid dynamics without masking the flame behavior by soot and radiative heat transfer. This choice of fuel also permits an evaluation of simulating low gravity in low pressure ground experiments because the similarity constraints are relaxed for the fast reacting, nonsooting diffusion flames. The diagnostics consists primarily of a color schlieren system coupled with computer generated rainbow filters, video recording, and image analysis. The project involves (1) drop tower experiments, (2) ground experiments, and (3) theoretical analysis.

Agrawal, Ajay K.↗

Vitamin D endocrine system after short-term space flight

The exposure of the body to microgravity during space flight causes a series of well-documented changes in Ca(2+) metabolism, yet the cellular/molecular mechanisms leading to these changes are poorly understood. There is some evidence for microgravity-induced alterations in the vitamin D endocrine system, which is known to be primarily involved in the regulation of Ca(2+) metabolism. Vitamin D-dependent Ca(2+) binding proteins, or calbindins, are believed to have a significant role in maintaining cellular Ca(2+) homeostasis. We used immunocytochemical, biochemical and molecular approaches to analyze the expression of calbindin-D(sub 28k) and calbindin-D(sub 9k) in kidneys and intestines of rats flown for 9 days aboard the Spacelab 3 mission. The effects of microgravity on calbindins in rats in space vs. 'grounded' animals (synchronous Animal Enclosure Module controls and tail suspension controls) were compared. Exposure to microgravity resulted in a significant decrease in calbindin-D(sub 28k) content in kidneys and calbindin-D(sub 9k) in the intestine of flight and suspended animals, as measured by enzyme-linked immunosorbent assay (ELISA). Immunocytochemistry (ICC) in combination with quantitative computer image analysis was used to measure in situ the expression of calbindins in kidneys and intestine, and insulin in pancreas. There was a large decrease in the distal tubular cell-associated calbindin-D(sub 28k) and absorptive cell-associated calbindin-D(sub 9k) immunoreactivity in the space and suspension kidneys and intestine, as compared with matched ground controls. No consistent differences in pancreatic insulin immunoreactivity between space, suspension and ground controls was observed. There were significant correlations between results by quantitative ICC and ELISA. Western blot analysis showed no consistent changes in the low levels of intestinal and renal vitamin D receptors. These findings suggest that a decreased expression of calbindins after a short-term exposure to microgravity and modelled weightlessness, may affect cellular Ca(2+) homeostasis and contribute to Ca(2+) and bone metabolism disorders induced by space flight.

Rhoten, William B.↗

Thrust Vectoring on the NASA F-18 High Alpha Research Vehicle

Investigations into a multiaxis thrust-vectoring system have been conducted on an F-18 configuration. These investigations include ground-based scale-model tests, ground-based full-scale testing, and flight testing. This thrust-vectoring system has been tested on the NASA F-18 High Alpha Research Vehicle (HARV). The system provides thrust vectoring in pitch and yaw axes. Ground-based subscale test data have been gathered as background to the flight phase of the program. Tests investigated aerodynamic interaction and vane control effectiveness. The ground-based full-scale data were gathered from static engine runs with image analysis to determine relative thrust-vectoring effectiveness. Flight tests have been conducted at the NASA Dryden Flight Research Center. Parameter identification input techniques have been developed. Individual vanes were not directly controlled because of a mixer-predictor function built into the flight control laws. Combined effects of the vanes have been measured in flight and compared to combined effects of the vanes as predicted by the cold-jet test data. Very good agreement has been found in the linearized effectiveness derivatives.

Bowers, Albion H.↗

Interaction of Light and Ethylene on Stem Gravitropism

The major objective of this study was to evaluate light-regulated ethylene production during gravitropic bending in etiolated pea stems. Previous investigations indicated that ethylene production increases after gravistimulation and is associated with the later (counter-reactive) phase of bending. Additionally, changes in the counter-reaction and locus of curvature during gravitropism are greatly influenced by red light and ethylene production. Ethylene production may be regulated by the levels of available precursor (1-aminocyclopropane-l-carboxylic acid, ACC) via its synthesis, conjugation to malonyl-ACC or glutamyl-ACC, or oxidation to ethylene. The regulation of ethylene production by quantifying ACC and conjugated ACC levels in gravistimulated pea stemswas examined. Also measured was the changes in protein and enzyme activity associated with gravitropic curvature by electrophoretic and spectrophotometric techniques. An image analysis system was used to visualize and quantify enzymatic activity and transcriptional products in gravistimulated and red-light treated etiolated pea stem tissues.

Harrison, Marcia A.↗

Probability Density Functions of Observed Rainfall in Montana

The question of whether a rain rate probability density function (PDF) can vary uniformly between precipitation events is examined. Image analysis on large samples of radar echoes is possible because of advances in technology. The data provided by such an analysis easily allow development of radar reflectivity factors (and by extension rain rate) distribution. Finding a PDF becomes a matter of finding a function that describes the curve approximating the resulting distributions. Ideally, one PDF would exist for all cases; or many PDF's that have the same functional form with only systematic variations in parameters (such as size or shape) exist. Satisfying either of theses cases will, validate the theoretical basis of the Area Time Integral (ATI). Using the method of moments and Elderton's curve selection criteria, the Pearson Type 1 equation was identified as a potential fit for 89 percent of the observed distributions. Further analysis indicates that the Type 1 curve does approximate the shape of the distributions but quantitatively does not produce a great fit. Using the method of moments and Elderton's curve selection criteria, the Pearson Type 1 equation was identified as a potential fit for 89% of the observed distributions. Further analysis indicates that the Type 1 curve does approximate the shape of the distributions but quantitatively does not produce a great fit.

Larsen, Scott D.↗

Mechanisms of Bone Mineralization and Effects of Mechanical Loading

The data suggest that PTH and PKC inhibit nodule formation, and that alternative energy sources are utilized by osteoblasts in the process of mineralization. The conditions and techniques to grow, fix, photograph, and measure bone mineralization in vitro were defined. The results are presently in preliminary form and require further assessment as follows; quantitate the surface area of nodules + treatments via computer-aided image analysis; use PTH + inhibitors of signaling pathways to determine the mechanism of nodule formation; determine how protein kinase C is involved as a promotor of nodule formation; cell proliferation vs. cell death affected by modulation of signal transduction (i.e., PTH, enzyme inhibitors and activators); identify mRNA induced or decreased in response to PTH and signaling modulators that encode proteins that regulate cell morphology, proliferation, and nodule formation. Therefore, several follow-up studies between the laboratories at NASA-Ames Research Center and my laboratory at the University of Illinois have been initiated.

Babich, Michael↗

Imaging Fluorescent Combustion Species in Gas Turbine Flame Tubes: On Complexities in Real Systems

Planar laser-induced fluorescence (PLIF) is used to visualize the flame structure via OH, NO, and fuel imaging in kerosene- burning gas turbine combustor flame tubes. When compared to simple gaseous hydrocarbon flames and hydrogen flames, flame tube testing complexities include spectral interferences from large fuel fragments, unknown turbulence interactions, high pressure operation, and the concomitant need for windows and remote operation. Complications of these and other factors as they apply to image analysis are considered. Because both OH and gas turbine engine fuels (commercial and military) can be excited and detected using OH transition lines, a narrowband and a broadband detection scheme are compared and the benefits and drawbacks of each method are examined.

Hicks, Y. R.↗

Endothelial Cell Morphology and Migration are Altered by Changes in Gravitational Fields

Endothelial cell migration is important to vascular wall regeneration following injury or stress. However, the mechanism(s) governing this response is not well understood. The microgravity environment of space may complicate the response of these cells to injury. To date, there are no reports in this area. We examined how bovine aortic (BAEC) and pulmonary (BPEC) endothelial cells respond to denudation injury under hypergravity (HGrav) and simulated microgravity (MGrav), using image analysis. In 10% FBS, the migration of confluent BAEC and BPEC into the denuded area was not affected by HGrav or MGrav. However, in low FBS (0.5%), signficantly retarded migration under MGrav, and increased migration under HGrav was found. MGrav also decreased the migration of postconfluent BPEC while HGrav showed no difference. Both MGrav and HGrav strongly decreased the migration of postconfluent BAEC. Also, both cell lines showed significant morphological changes by scanning electron microscopy. These studies indicate that endothelial cell function is affected by changes in gravity.

Melhado, Caroline↗

Measurements of the Viscosity of the Undercooled Melts Under the Conditions of Microgravity and Supporting MHD Calculations

This report covers the work to support the measurement of the surface tension and viscosity of undercooled metals with the TEMPUS as a part of the IML-2 mission. This work consisted of two parts. First was the ground-based research program, whose purpose was to establish the feasibility of the flight program and to establish the knowledge base necessary to plan a successful flight program. Analytical calculations established a fundamental understanding of the problem, and then a more rigorous program of numerical calculations was employed in the development of the experimental program. The second part of the covered research was the actual flight program, developed in close collaboration with Prof. Egry. A metal sphere was levitated and melted, and then set oscillating by 'squeezing' it with the magnetic field. The droplet's oscillations were recorded on videotape and digital image analysis was employed in reduction of the data. The experiment was successful because surface tension measurements were obtained for the gold and gold-copper alloy samples. This data provides unique experimental evidence in support of theories about electromagnetic levitation on the ground. Because of unforeseen difficulties with stability of liquid samples in TEMPUS, the viscosity measurements were not possible, but the data collection and analysis techniques were well proven in this mission. Further research is being conducted regarding the measurement of viscosity by this technique.

Szekely, Julian↗

Use of Microgravity to Control the Microstructure of Eutectics

This grant began in June of 1996. Its long term goal is to be able to control the microstructure of directionally solidified eutectic alloys, through an improved understanding of the influence of convection. The primary objective of the present projects is to test hypotheses for the reported influence of microgravity on the microstructure of three fibrous eutectics (MnBi-Bi, InSb-NiSb, Al3Ni-Al). A secondary objective is to determine the influence of convection on the microstructure of other eutectic alloys. Two doctoral students and a masters student supported as a teaching assistant were recruited for this research. Techniques were developed for directional solidification of MnBi-Bi eutectics with periodic application of current pulses to produce an oscillatory freezing rate. Image analysis techniques were developed to obtain the variation in MnBi fiber spacing, which was found to be normally distributed. The mean and standard deviation of fiber spacing were obtained for several freezing conditions. Eighteen ampoules were prepared for use in the gradient freeze furnace QUELD developed at Queen's University for use in microgravity. Nine of these ampoules will be solidified soon at Queen's in a ground-based model. We hope to solidify the other nine in the QUELD that is mounted on the Canadian Microgravity Isolation Mount on MIR. Techniques are being developed for directional solidification of the Al-Si eutectic at different freezing rates, with and without application of accelerated crucible rotation to induce convection. For the first time, theoretical methods are being developed to analyze eutectic solidification with an oscillatory freezing rate. In a classical sharp-interface model, we found that an oscillatory freezing rate increases the deviation of the average interfacial composition from the eutectic, and increases the undercooling of the two phases by different amounts. This would be expected to change the volume fraction solidifying and the fiber spacing. Because of difficulties in tracking the freezing interfaces of the two solid phases, a phase-field model is also being developed. A paper demonstrating application of phase field methods to periodic structures has been submitted for publication.

Wilcox, William R.↗

Thermomechanical Fatigue Durability of T650-35/PMR-15 Sheet Molding Compound

Although polyimide based composites have been used for many years in a wide variety of elevated temperature applications, very little work has been done to examine the durability and damage behavior under more prototypical thermomechanical fatigue (TMF) loadings. Synergistic effects resulting from simultaneous temperature and load cycling can potentially lead to enhanced, if not unique, damage modes and contribute to a number of nonlinear deformation responses. The goal of this research was to examine the effects of a TMF loading spectrum, representative of a gas turbine engine compressor application, on a polyimide sheet molding compound (SMC). High performance SMCs present alternatives to prepreg forms with great potential for low cost component production through less labor intensive, more easily automated manufacturing. To examine the issues involved with TMF, a detailed experimental investigation was conducted to characterize the durability of a T650-35/PMR-15 SMC subjected to TMF mission cycle loadings. Fatigue damage progression was tracked through macroscopic deformation and elastic stiffness. Additional properties, such as the glass transition temperature (T(sub g) and dynamic mechanical properties were examined. The fiber distribution orientation was also characterized through a detailed quantitative image analysis. Damage tolerance was quantified on the basis of residual static tensile properties after a prescribed number of TMF missions. Detailed microstructural examinations were conducted using optical and scanning electron microscopy to characterize the local damage. The imposed baseline TMF missions had only a modest impact on inducing fatigue damage with no statistically significant degradation occurring in the measured macroscopic properties. Microstructural damage was, however, observed subsequent to 100 h of TMF cycling which consisted primarily of fiber debonding and transverse cracking local to predominantly transverse fiber bundles. The TMF loadings did introduce creep related effects (strain accumulation) which led to rupture in some of the more aggressive stress scenarios examined. In some cases this creep behavior occurred at temperatures in excess of 150 C below commonly cited values for T(sub g). Thermomechanical exploratory creep tests revealed that the SMC was subject to time dependent deformation at stress/temperature thresholds of 150 MPa/230 C and 170 MPa/180 C.

Castelli, Michael G.↗

Analytical Tools for Cloudscope Ice Measurement

The cloudscope is a ground or aircraft instrument for viewing ice crystals impacted on a sapphire window. It is essentially a simple optical microscope with an attached compact CCD video camera whose output is recorded on a Hi-8 mm video cassette recorder equipped with digital time and date recording capability. In aircraft operation the window is at a stagnation point of the flow so adiabatic compression heats the window to sublimate the ice crystals so that later impacting crystals can be imaged as well. A film heater is used for ground based operation to provide sublimation, and it can also be used to provide extra heat for aircraft operation. The compact video camera can be focused manually by the operator, and a beam splitter - miniature bulb combination provide illumination for night operation. Several shutter speeds are available to accommodate daytime illumination conditions by direct sunlight. The video images can be directly used to qualitatively assess the crystal content of cirrus clouds and contrails. Quantitative size spectra are obtained with the tools described in this report. Selected portions of the video images are digitized using a PCI bus frame grabber to form a short movie segment or stack using NIH (National Institute of Health) Image software with custom macros developed at DRI. The stack can be Fourier transform filtered with custom, easy to design filters to reduce most objectionable video artifacts. Particle quantification of each slice of the stack is performed using digital image analysis. Data recorded for each particle include particle number and centroid, frame number in the stack, particle area, perimeter, equivalent ellipse maximum and minimum radii, ellipse angle, and pixel number. Each valid particle in the stack is stamped with a unique number. This output can be used to obtain a semiquantitative appreciation of the crystal content. The particle information becomes the raw input for a subsequent program (FORTRAN) that synthesizes each slice and separates the new from the sublimating particles. The new particle information is used to generate quantitative particle concentration, area, and mass size spectra along with total concentration, solar extinction coefficient, and ice water content. This program directly creates output in html format for viewing with a web browser.

Arnott, W. Patrick↗

Quantitative Method of Measuring Metastatic Activity

The metastatic potential of tumors can be evaluated by the quantitative detection of urokinase and DNA. The cell sample selected for examination is analyzed for the presence of high levels of urokinase and abnormal DNA using analytical flow cytometry and digital image analysis. Other factors such as membrane associated uroldnase, increased DNA synthesis rates and certain receptors can be used in the method for detection of potentially invasive tumors.

Morrison, Dennis R.↗

A Noncontact Measurement Technique for the Density and Thermal Expansion Coefficient of Solid and Liquid Materials

A noncontact measurement technique for the density and the thermal expansion refractory materials in their molten as well as solid phases is presented. This technique is based on the video image processing of a levitated sample. Experiments were performed using the high-temperature electrostatic levitator (HTESL) at the Jet Propulsion Laboratory in which 2-3 mm diameter samples can be levitated, melted, and radiatively cooled in a vacuum. Due to the axisymmetric nature of the molten samples when levitated in the HTESL, a rather simple digital image analysis can be employed to accurately measure the volumetric change as a function of temperature. Density and the thermal expansion coefficient measurements were made on a pure nickel sample to test the accuracy of the technique in the temperature range of 1045-1565 C. The result for the liquid phase density can be expressed by p = 8.848 + (6.730 x 10(exp -4)) x T (degC) g/cu cm within 0.8% accuracy, and the corresponding thermal expansion coefficient can be expressed by Beta=(9.419 x 10(exp -5)) - (7.165 x 10(exp -9) x T (degC)/K within 0.2% accuracy.

Chung, Sang K.↗

Computational Microbial Morphometry and NASA Astrobiology Initiatives

From the 12 known meteorites believed to have made their way to Earth from Mars, about 20 kg (44 lbs.) of material are suitable for searching for microfossil evidence. An automated neural network trained to identify common morphologies to distinguish organic and non-organic origins for rock fossils is described. The high success rate of classification by this computerized image analysis (85% on training data) moves toward a fully-automated search technique.

Noever, David A.↗

Remote Sensing in Geography in the New Millennium: Prospects, Challenges, and Opportunities

Remote sensing science contributes greatly to our understanding of the Earth's ecosystems and cultural landscapes. Almost all the natural and social sciences, including geography, rely heavily on remote sensing to provide quantitative, and indispensable spatial information. Many geographers have made significant contributions to remote sensing science since the 1970s, including the specification of advanced remote sensing systems, improvements in analog and digital image analysis, biophysical modeling, and terrain analysis. In fact, the Remote Sensing Specialty Group (RSSG) is one of the largest specialty groups within the AAG with over 500 members. Remote sensing in concert with a geographic information systems, offers much value to geography as both an incisive spatial-analytical tool and as a scholarly pursuit that adds to the body of geographic knowledge on the whole. The "power" of remote sensing as a research endeavor in geography lies in its capabilities for obtaining synoptic, near-real time data at many spatial and temporal scales, and in many regions of the electromagnetic spectrum - from microwave, to RADAR, to visible, and reflective and thermal infrared. In turn, these data present a vast compendium of information for assessing Earth attributes and characte6stics that are at the very core of geography. Here we revisit how remote sensing has become a fundamental and important tool for geographical research, and how with the advent of new and improved sensing systems to be launched in the near future, remote sensing will further advance geographical analysis in the approaching New Millennium.

Quattrochi, Dale A.↗