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At least 577 records · Page 32

Early cellular evolution.

Study of the evolutionary developments that occurred subsequent to the origin of ancestral cells. Microbial physiology and ecology are potential sharp tools for shaping concepts of microbial evolution. Some popular unjustified assumptions are discussed. It is considered that certain principles derived mainly from the advances of molecular biology can be used to order the natural groups (genera) of extant prokaryotes and their patterns phylogenetically.

Margulis, L.↗

The physics of cellular synthesis, growth and division

Three areas of research in NASA'S University Program are described. Primitive terrestrial living cells were studied as a guide to the kind of cells to look for in extraterrestrial life. Experiments in zero gravity conditions are described with emphasis upon effects on small organisms. The effects of ionizing radiation on cells are studied so that it will be possible to predict dosages which can be tolerated by humans with no permanent damage.

Pollard, E. C.↗

Nonlinear cellular motions in Poiseuille channel flow

In a number of nonlinear solutions to the equations of channel flow the velocity is decomposed into a mean part plus a nonlinear disturbance. The idea that nonlinear effects place a limitation on the amplitudes of the disturbance flow is considered. In the reported investigation the disturbance flow is represented by drastically limited Fourier expansions in the downstream coordinate. The resulting equations are solved numerically with high accuracy to obtain a good representation of the cross-stream structure of the solution. The results of the investigation show that indeed the nonlinear terms always limit the amplitude of the disturbance flow in this approximation.

Zahn, J.-P.↗

Early detection of disease program: Evaluation of the cellular immune response

Surfaces of normal, cultured, and mitogen-stimulated mouse lymphoid cells were examined by scanning electron microscopy (SEM). Lymphocytes with smooth, highly villous and intermediate surfaces were observed in cell suspensions from both spleens and thymuses of normal mice and from spleens of congenitally athymic (nude) mice. Several strain-specific surface features were noted, including the spine-like appearance of microvilli on C57B1/6 lymphocytes. Although thymus cell suspensions contained somewhat more smooth cells than did spleen cell preparations, lymphocyte derivation could not be inferred from SEM examination. Studies of cells stimulated with mitogenic agents for thymus-derived lymphocytes (concanavalin A) or for bone marrow-derived lymphocytes (lipopolysaccharide) suggested that, in the mouse, development of a complex villous surface is a general concomitant of lymphocyte activation and transformation.

Criswell, B. S.↗

The effect of changes in the USF/NASA toxicity screening test method on data from some cellular polymers

Rankings of relative toxicity can be markedly affected by changes in test variables. Revision of the USF/NASA toxicity screening test procedure to eliminate the connecting tube and supporting floor and incorporate a 1.0 g sample weight, 200 C starting temperature, and 800 C upper limit temperature for pyrolysis, reversed the rankings of flexible polyurethane and polychloroprene foams, not only in relation to each other, but also in relation to cotton and red oak. Much of the change is attributed to reduction of the distance between the sample and the test animals, and reduction of the sample weight charged. Elimination of the connecting tube increased the relative toxicity of the polyurethane foams. The materials tested were flexible polyurethane foam, without and with fire retardant; rigid polyurethane foam with fire retardant; flexible polychloroprene foam; cotton, Douglas fir, red oak, hemlock, hardboard, particle board, polystyrene, and polymethyl methacrylate.

Hilado, C. J.↗

Is gravity a morphological determinant in plants at the cellular level

The present paper deals with the question whether plant development can proceed normally in the weightless state, particularly in the critical stage where single cells produce multicellular units, leading to embryos with the growing regions of shoot and root which, in turn, give rise to all the tissues of the plant body. An experiment that tested whether carrot embryos capable of developing from cultured somatic cells could do so under conditions of weightlessness is described.

Krikorian, A. D.↗

Impact of cell size on inventory and mapping errors in a cellular geographic information system

The author has identified the following significant results. The effect of grid position was found insignificant for maps but highly significant for isolated mapping units. A modelable relationship between mapping error and cell size was observed for the map segment analyzed. Map data structure was also analyzed with an interboundary distance distribution approach. Map data structure and the impact of cell size on that structure were observed. The existence of a model allowing prediction of mapping error based on map structure was hypothesized and two generations of models were tested under simplifying assumptions.

Wehde, M. E.↗

Evaluation of the effects of a freeze/thaw environment on cellular glass

Using the evaluation criteria of water vapor permeability and conformability, a protective butylrubber/silicone conformal coating system was selected for use on Foamglas substrates in a freeze/thaw environment. The selection of a specific freeze/thaw cycle which closely models field conditions is discussed. A sampling plan is described which allows independent evaluation of the effects of conformal coatings, cycle number and location within the environmental chamber. The results of visual examination, measurement of density, modulus of rupture and Young's modulus are reported. Based upon statistical evaluation of the experimental results, it is concluded that no degradation in mechanical properties of either coated or uncoated Foamglas occurred within the duration of the test (53 freeze/thaw cycles).

Frickland, P.↗

The assembly and properties of protobiological structures - The beginnings of cellular peptide synthesis

New data indicate that lysine-rich proteinoids have the ability to catalyze the synthesis of peptide bonds from a variety of amino acids and ATP. This capacity is evident in aqueous solution, in suspension of phase-separated complexes of lysine-rich proteinoid with acidic proteinoids, and in suspension of phase-separated particles composed of lysine-rich proteinoids with polynucleotides. Since the proteinoid complexes can contain other catalytic activities, including ability to catalyze internucleotide bond formation, it is inferred that the first protocells on earth already had a number of biological types of activity.

Fox, S. W.↗

Phylogenetic sequence of metabolic pathways in Precambrian cellular life

A sequence of major metabolic events is presented as they may have appeared during prokaryote evolution. This is based on (1) the phylogenetic schema derived from sequences of bacterial ferredoxin, 2Fe-2S ferredoxin, 5S ribosomal RNA, and c-type cytochromes; (2) metabolic settings in which these macromolecules are found; and (3) metabolic capabilities of the prokaryotes that carry these molecules.

Barnabas, J.↗

Freeze/Thaw Properties of Cellular Glass

Without moisture, temperature cycles do no harm. Experiments described in new report indicate inherent material variability greater deterrent to application of material than freeze/thaw effects.

Frickland, P. O.↗

Fabrication of Structural Cellular Glass

Surface layer quickly heated and compressed. Postcellulation skin densification uses gas burners to head lower surface of material and pinch rollers to compress heated-surface layer. Useful for manufacturing large low cost, parabolic dish solar collectors.

Mitchell, W. D.↗

Calcium movements and the cellular basis of gravitropism

Evidence that calcium is a significant factor in gravitropism of plants is examined, together with the regulatory mechanism that controls calcium efflux in plant cells. Calcium has been found to accumulate on the upper surface of a plant placed in a horizontal attitude, which is followed by growth to the vertical within a half-hour. It has also been determined that the upper surface of the plant simultaneously experiences a reduced growth rate, compared to the lower surface. The substance calmodulin has been identified as the calcium-binding regulatory protein most influential in the transduction process leading to gravitropism, while chlorpromazine can inhibit its effects. The calcium is transported by ATPase located in the cell membrane. A possibility that the calcium pump is regulated by a feedback mechanism involving the presence of calmodulin is discussed.

Roux, S. J.↗

Human cellular immune responsiveness following space flight

Peripheral circulating lymphocytes were separated from astronaut blood samples three times before and two times after the first four US Space Shuttle flights. The ability of the in vitro T lymphocytes to respond to Phytohemagglutinin by blastogenesis was found to be reduced for each crewmember following spaceflight. In addition, the astronauts experienced a postflight increase in neutrophils and a decrease in eosinophils. These postflight changes in leukocytes are shown to increase with subjectively-evaluated increases in the incidence of inflight stress, indicating that stress, and not hypogravity, is likely to be the major effector of these changes.

Taylor, G. R.↗

Penetrative cellular convection in a stratified atmosphere

In the present investigation of penetrative convection within a simple compressible model, the middle one of the three layers of differing stratification prior to the onset of convection is a convectively unstable polytrope bounded above and below by two stably stratified polytropes. One- and two-mode steady solutions with hexagonal planforms have been studied for Rayleigh numbers up to aobut 1000 times critical, and for a range of Prandtl numbers, horizontal wavenumbers, and stratifications. These indicate that the penetration into the lower stable layer by downward plumes is substantially larger in a stratified medium than in a Boussinesq fluid, and produces an extended region of adiabatic stratification. The strong asymmetry between upward and downward penetration in compressible media has major implications for the mixing of stable regions above and below stellar convection zones.

Massaguer, J. M.↗

Cellular monotectic model solidification

Succinonitrile (sn) was purified to a superior level using a fractional recrystallization method. The melting point of the best twice recrystallized sn was not raised by following with double distillation. This was tested using differential scanning calorimetry. The peak shape on melting also proved that double distillation after double recrystallization did not improve the quality. Stability and phase diagrams for succinonitrile and glycerol are presented.

Kaukler, William F.↗