cDNA normalization by hydroxyapatite chromatography to enhance transcriptome diversity in RNA-Seq Applications.
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The present application relates to microfabricated columns having a sealed flow channel. In particular, the channel is sealed during dicing, thereby preventing debris from accumulating within channels. In use, the seal is ruptured mechanically to connect one or more fluidic connections, which deliver analytes to the flow channel. Additional details are provided for making and using sealed columns.
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