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At least 559 records · Page 31

Delayed expression of hpS2 and prolonged expression of CIP1/WAF1/SDI1 in human tumour cells irradiated with X-rays, fission neutrons or 1 GeV/nucleon Fe ions

PURPOSE: Differences in gene expression underlie the phenotypic differences between irradiated and unirradiated cells. The goal was to identify late-transcribed genes following irradiations differing in quality, and to determine the RBE of 1 GeV/n Fe ions. MATERIALS AND METHODS: Clonogenic assay was used to determine the RBE of Fe ions. Differential hybridization to cDNA target clones was used to detect differences in expression of corresponding genes in mRNA samples isolated from MCF7 cells irradiated with iso-survival doses of Fe ions (0 or 2.5 Gy) or fission neutrons (0 or 1.2 Gy) 7 days earlier. Northern analysis was used to confirm differential expression of cDNA-specific mRNA and to examine expression kinetics up to 2 weeks after irradiation. RESULTS: Fe ion RBE values were between 2.2 and 2.6 in the lines examined. Two of 17 differentially expressed cDNA clones were characterized. hpS2 mRNA was elevated from 1 to 14 days after irradiation, whereas CIP1/WAF1/SDI1 remained elevated from 3 h to 14 days after irradiation. Induction of hpS2 mRNA by irradiation was independent of p53, whereas induction of CIP1/WAF1/SDI1 was observed only in wild-type p53 lines. CONCLUSIONS: A set of coordinately regulated genes, some of which are independent of p53, is associated with change in gene expression during the first 2 weeks post-irradiation.

NASA Discipline Radiation Health↗

Astrometric Planet Searches with SIM PlanetQuest

SIM will search for planets with masses as small as the Earth's orbiting in the habitable zones' around more than 100 of the stars and could discover many dozen if Earth-like planets are common. With a planned 'Deep Survey' of 100-450 stars (depending on desired mass sensitivity) SIM will search for terrestrial planets around all of the candidate target stars for future direct detection missions such as Terrestrial Planet Finder and Darwin, SIM's 'Broad Survey' of 2010 stars will characterize single and multiple-planet systems around a wide variety of stellar types, including many now inaccessible with the radial velocity technique. In particular, SIM will search for planets around young stars providing insights into how planetary systems are born and evolve with time.

formation solar system↗

Sensors Provide Early Warning of Biological Threats

Early Warning Inc. of Troy, New York, licensed powerful biosensor technology from Ames Research Center. Incorporating carbon nanotubes tipped with single strands of nucleic acid from waterborne pathogens, the sensor can detect even minute amounts of targeted, disease causing bacteria, viruses, and parasites. Early Warning features the NASA biosensor in its water analyzer, which can provide advance alert of potential biological hazards in water used for agriculture, food and beverages, showers, and at beaches and lakes -- within hours instead of the days required by conventional laboratory methods.

Source record↗

Agile Science Operations: A New Approach for Primitive Exploration Bodies

Primitive body exploration missions such as potential Comet Surface Sample Return or Trojan Tour and Rendezvous would challenge traditional operations practices. Earth-based observations would provide only basic understanding before arrival and many science goals would be defined during the initial rendezvous. It could be necessary to revise trajectories and observation plans to quickly characterize the target for safe, effective observations. Detection of outgassing activity and monitoring of comet surface activity are even more time constrained, with events occurring faster than round-trip light time. "Agile science operations" address these challenges with contingency plans that recognize the intrinsic uncertainty in the operating environment and science objectives. Planning for multiple alternatives can significantly improve the time required to repair and validate spacecraft command sequences. When appropriate, time-critical decisions can be automated and shifted to the spacecraft for immediate access to instrument data. Mirrored planning systems on both sides of the light-time gap permit transfer of authority back and forth as needed. We survey relevant science objectives, identifying time bottlenecks and the techniques that could be used to speed missions' reaction to new science data. Finally, we discuss the results of a trade study simulating agile observations during flyby and comet rendezvous scenarios. These experiments quantify instrument coverage of key surface features as a function of planning turnaround time. Careful application of agile operations techniques can play a significant role in realizing the Decadal Survey plan for primitive body exploration

Agile science↗

The NASA Meter Class Autonomous Telescope: Ascension Island

The Meter Class Autonomous Telescope (MCAT) is the newest optical sensor dedicated to NASA s mission to characterize the space debris environment. It is the successor to a series of optical telescopes developed and operated by the JSC Orbital Debris Program Office (ODPO) to monitor and assess the debris environment in (1) Low Earth Orbit (LEO), (2) Medium Earth Orbit (MEO), and (3) Geosynchronous Orbit (GEO), with emphasis on LEO and GEO altitudes. A joint NASA-Air Force Research Labs project, MCAT is a 1.3m optical telescope dedicated to debris research. Its optical path and sensor yield a large survey fence at the cutting edge of current detector performance. It has four primary operational observing modes, two of which were not computationally feasible a decade ago. Operations are supported by a sophisticated software suite that monitors clouds and weather conditions, and controls everything from data collection to dome rotation to processing tens of GB of imagery data nightly. With fainter detection limits, precision detection, acquisition and tracking of targets, multi-color photometry, precision astrometry, automated reacquisition capability, and the ability to process all data at the acquisition rate, MCAT is capable of producing and processing a volume and quality of data far in excess of any current (or prior) ODPO operations. This means higher fidelity population inputs and eliminating the multi-year backlog from acquisition-to-product typical of optical campaigns. All of this is possible given a suitable observing location. Originally planned for the island of Legan, part of the Kwajalein Atoll Islands, recent developments have led to a change in venue. Specifically, the Ground-based Electro-Optical Deep Space Surveillance, or GEODSS, System of telescopes is the United States major tracking system for deep space. This network consists of telescopes in Maui, Hawaii; Diego Garcia (Indian Ocean), and Socorro, New Mexico. A fourth optical telescope, though smaller in size, has been operating in conjunction with this effort until recently in M.ron, Spain. With the M.ron site closing, a significant gap in longitude exists between the New Mexico and Diego Garcia sites. This longitudinal gap is well covered by placing a telescope on Ascension Island (7degrees 58'20" S, 14degrees 24'4"W), in the Atlantic Ocean. Ascension Island offers the benefits of both location and weather. The near equatorial location affords the opportunity to access under-sampled low-inclination orbits and new GEO longitudes, while simultaneously filling in the GEODSS longitudinal gap. Ascension Island is a volcanic, desert island, receiving only 7" of rain per year on average. With consistent trade winds blowing from the SSE direction off Africa, the combination of an island location with consistent winds will create the smooth laminar flow sought after by all astronomical sites, which creates stable atmospheric ('seeing') conditions. Finally, this low population island has minimal lighting, resulting in very dark skies, ideal for an observatory.

Lederer, S. M.↗

The NASA Meter Class Autonomous Telescope: Ascension Island

The Meter Class Autonomous Telescope (MCAT) is the newest optical sensor dedicated to NASA's mission to characterize the space debris environment. It is the successor to a series of optical telescopes developed and operated by the JSC Orbital Debris Program Office (ODPO) to monitor and assess the debris environment in (1) Low Earth Orbit (LEO), (2) Medium Earth Orbit (MEO), and (3) Geosynchronous Orbit (GEO), with emphasis on LEO and GEO altitudes. A joint NASA - Air Force Research Labs project, MCAT is a 1.3m optical telescope dedicated to debris research. Its optical path and sensor yield a large survey fence at the cutting edge of current detector performance. It has four primary operational observing modes, two of which were not computationally feasible a decade ago. Operations are supported by a sophisticated software suite that monitors clouds and weather conditions, and controls everything from data collection to dome rotation to processing tens of gigabytes of image data nightly. With fainter detection limits, precision detection, acquisition and tracking of targets, multi-color photometry, precision astrometry, automated re-acquisition capability, and the ability to process all data at the acquisition rate, MCAT is capable of producing and processing a volume and quality of data far in excess of any current (or prior) ODPO operations. This means higher fidelity population inputs and eliminating the multi-year backlog from acquisition-to-product typical of optical campaigns. All of this is possible given a suitable observing location. Ascension Island offers numerous advantages. As a British overseas territory with a US Air Force base presence, the necessary infrastructure and support already exists. It is located mid-way between Brazil and Africa at 7.93S latitude and 14.37 W longitude. With the Ground-based Electro-Optical Deep Space Surveillance (GEODSS) asset in Moron, Spain shutting down, this presents access to the sky from a unique latitude/longitude for an optical telescope. Constant trade winds from the SSE, originating from Africa, give promise to a steady laminar airflow over an island, a trait sought after to create stable atmospheric and good astronomical 'seeing' conditions with very low annual rainfall values. This combination of attributes created the necessary compelling argument to redirect MCAT to its final destination: Ascension Island.

Lederer, S. M.↗

Wald Sequential Probability Ratio Test for Space Object Conjunction Assessment

This paper shows how satellite owner/operators may use sequential estimates of collision probability, along with a prior assessment of the base risk of collision, in a compound hypothesis ratio test to inform decisions concerning collision risk mitigation maneuvers. The compound hypothesis test reduces to a simple probability ratio test, which appears to be a novel result. The test satisfies tolerances related to targeted false alarm and missed detection rates. This result is independent of the method one uses to compute the probability density that one integrates to compute collision probability. A well-established test case from the literature shows that this test yields acceptable results within the constraints of a typical operational conjunction assessment decision timeline. Another example illustrates the use of the test in a practical conjunction assessment scenario based on operations of the International Space Station.

Navigation↗

An Integrated System for Autonomous Search and Track with a Small Unmanned Aerial Vehicle

This article presents a framework for searching, detecting, and tracking an object of interest with a small unmanned aerial vehicle (sUAV). The vehicle is given an area to search for an object of interest. Once the object is detected, the sUAV follows the target while maintaining a fixed distance and centered on its image plane. This paper describes an architecture for integrating autonomy capabilities for search and track for sUAVs. The system has been implemented in the Robot Operating System (ROS) framework using the Parrot ARDrone platform. In this paper we present our technical approach, the system architecture and demonstrate the principles of the approach in a simulation environment and indoor flight tests.

Unmanned Aerial Vehicle↗

LEAVES: Lofted Environmental and Atmospheric Venus Sensors

LEAVES (Lofted Environmental Atmospheric Venus Sensors) is a design exercise with the goal of dramatically decreasing the cost of obtaining prioritized chemical and physical data in planetary atmospheres. Through the application of a swarm approach this concept parallelizes atmospheric exploration, with geographic coverage far exceeding what is possible with conventional monolithic platforms or sondes. Each unit in the swarm is exceptionally compact, with a powered payload mass of only a few tens of grams and a high-drag, semi-rigid structure that acts to slow each probe as it descends through the atmosphere. This structural design can collapse into a planar form to allow for efficient stowage prior to arrival at the target body. With a total per-unit mass of only 120 g, a fleet of 100 (or more) units can be very reasonably accommodated on a carrier spacecraft.Science operations, which begin when the LEAVES probes reach an altitude of 100 km, are targeted for the cloud-bearing region of Venus' atmosphere. During the roughly 9 hour, terminal velocity descent through the atmosphere, LEAVES collects data of the state and composition of the atmosphere in parallel across multiple units. These data would represent an unprecedented constraint on the distribution and concentration of targeted chemical species, and the detection of local and regional variations in both chemistry and physical properties.A novel and compelling result of this exercise was that the same optimization that produced a structure with an exceptionally low areal mass density (0.126 kg/m2) also resulted in a probe that can be deployed directly from an aerobraking orbit (~140 km at 5 km/s) without the need for aeroshell protection. This translates to a tremendous mass savings and gives LEAVES the flexibility to be carried as a secondary payload aboard either a descending surface probe or an orbital radar mapper. Because such missions are under active development or have already been proposed (but not flown), we infer that LEAVES is well positioned as a technology

Swarm↗

NASA's Orbital Debris Optical Program: MCAT Operational on Ascension

The Orbital Debris Program Office at NASA Johnson Space Center has a long history of an optical observational program. The Meter Class Autonomous Telescope, MCAT, was dedicated to Eugene Stansbery (now also known as ES-MCAT) in 2017. MCAT, a 1.3m DFM telescope, has a proven capability for tracking known objects from Low-Earth Orbits (LEO) out to Geosynchronous (GEO) orbits. Monitoring the population of the GEO belt is accomplished through surveys. A GEO survey statistically samples the GEO belt (0 to ~15 deg orbital inclinations) to detect both correlated and uncorrelated targets. A GEO survey, the initial focus for MCAT, will commence in 2019 to map out the current state of the GEO population as input for the ORbital Debris Engineering Model (ORDEM 4.x). If a break-up occurs, surveys of the break-up field can be followed for discovery and investigations of daughter debris fragments from the parent satellite. Discovery can be accomplished by surveying orbits near to and including the parent object’s orbit. Targeted observations of debris can be taken with a suite of broadband filters for characterizing individual objects by rate-tracking their known or calculated orbital elements (Two-Line Element sets, TLEs). These observations can be used in conjunction with NASA’s Standard Satellite Break-up Model (SSBM). In 2018, MCAT’s primary mirror was realuminized with a high-end protected, enhanced silver ZeCoat and the CCD chip was replaced in the Spectral Instruments camera. With these updates completed, MCAT is now well on track to reach Full Operational Capability (FOC) in 2019 for its survey and rate-track capabilities. A full overview of MCAT’s operational state, capabilities, and mission will be discussed.

Orbital Debris Program↗

NASA's Orbital Debris Optical Program: ES-MCAT Updated and Upgraded

The 1.3m ES-MCAT telescope (or MCAT for short) now has a proven capability for observing objects from Low- Earth Orbit (LEO) out to Geosynchronous (GEO) orbit, and the ability to run all systems autonomously. A GEO survey, the initial focus for MCAT, will commence in late 2019 to map out the current state of the GEO population as input for the ORbital Debris Engineering Model (ORDEM 4.x). This survey will statistically sample the GEO belt (0 to ~15 deg orbital inclinations) to detect both known and unknown targets. If a break-up occurs, additional surveys of the break-up field can be followed for discovery and investigations of daughter debris fragments from the parent satellite. Discovery can be accomplished by tracking orbits near to and including the parent object’s orbit. Targeted observations of debris can be taken with a suite of broadband filters for characterizing individual objects by ratetracking their known or calculated orbital elements (Two-Line Element sets, TLEs). Several modifications and upgrades have been made to the instrumentation and systems originally installed in 2015 and are reported here. In 2018, MCAT’s primary mirror was recoated with a high-end protected, enhanced silver by the ZeCoat Corporation. The CCD chip was replaced in the Spectral Instruments camera with a broad-band antireflective coated chip. The automated weather systems have been modified from the original system, removing some weather sensors and installing replacements that are better suited to Ascension’s weather and environment. A new 2.5-m ObservaDome replaced the Astrohaven dome on the nearby tower platform that will house an auxiliary 0.4-meter telescope. Finally, in 2019, the Observatory Control System was upgraded to 2.0 which includes additional flexibility for automating data collection and reduction. With these updates completed, MCAT is now well on track to reach Full Operational Capability (FOC) in 2019 for its survey, rate-track, and TLE tracking capabilities.

Lederer, S. M.↗

Spacecraft Contamination Control Challenges for Space Missions with Organic Compound Detection Capabilities and for Potential Sample Return

Contamination control is critical to missions with organic compound detection capabilities, and for missions targeting acquisition of samples for potential return to Earth. Significant challenges are being addressed in the design of current flight projects and conceptual mission studies at JPL. These challenges extend to both orbiting spacecraft, as well as landed missions, for future missions to Mars and Europa, and potential missions to Titan and Enceladus. Contamination control during all phases of a mission, from preliminary design through operation, is fundamental to ensure that organic compounds of terrestrial origin are controlled to ensure successful completion of science objectives. This presentation summarizes contamination control challenges specific to landed missions (which include sample acquisition, encapsulation, caching, potential sample return, and UV instruments), and orbiting missions (modeling interactions between the spacecraft, and local exospheres and plumes).

Soares, Carlos↗

Characterize Planetary Surfaces from a Single Point to a Large Area Using a Standoff Ultra-Compact µ-Raman Instrument

A standoff Ultra-Compact micro-Raman (SUCR) instrument is used for detection of minerals, organic, and biological materials in daylight and nighttime conditions. The standoff measurements from a SUCR instrument based on micro-Raman spectroscopy on a rover or lander platform is ideal for identifying various types of ices, minerals, organics and biogenic materials. The μ-Raman measurements can assist in determining the biological potential for habitability assessment of planetary bodies as well as amino acid detection for evidence of past or present life on Mars, Europa, and other solar bodies. The SUCR instrument is used to inspect minerals, organic, biomarkers, ice, and embedded materials inside ice, as well as to conduct Raman line scanning from a single point to a large area for standoff detection at a 6-cm target distance.

M Nurul Abedin↗

Systems and Implementation: Integrating Unmanned Aircraft Systems into Physical Protection Systems at Fixed Sites and During Transportation

Physical protection systems, and response forces in particular, are designed to prevent an adversary from successfully completing a malevolent act against a facility or transport operations. Timely detection and assessment of any potential adversary action against a target is an essential element of materials security. The timely detection and assessment must then be followed-up by a capable and timely response that might be enhanced with the additional situational awareness provided by unmanned aircraft systems (UAS). The United States Department of Energy’s National Nuclear Security Administration Office of International Nuclear Security has been exploring capabilities provided by UAS to support response force operations within the physical protection system. UAS have the potential to provide response force commanders and operators with situational awareness in assessing adversary locations and actions as well as the locations of responders. UAS may be utilized for area searches ahead of responder pathways to identify potential threats and to provide situational awareness of areas not normally covered by cameras (such as areas outside the fence line outside at fixed facilities). In addition, UAS can provide real-time information to transportation convoy teams that pass through constantly changing public access environments. This paper will provide operational recommendations to be addressed when integrating UAS into existing physical protection systems at fixed sites and during transport. Recommendations will include aspects of the following: needs analysis; tactics and techniques to support detection and assessment as well as response force deployment; remote pilot selection, qualifications, training, and currency; UAS selection criteria; UAS laws and regulations; possible cost sharing with other facility operations; and on-scene emergency management.

Stockwell, Brandon↗

Low-energy nuclear recoil calibration of the LUX-ZEPLIN experiment with a photoneutron source

The LZ experiment is a liquid xenon time-projection chamber (TPC) searching for evidence of particle dark matter interactions. In the simplest assumption of elastic scattering, many dark matter models predict an energy spectrum which rises quasi-exponentially with decreasing energy transfer to a target atom. LZ expects to detect coherent neutrino-nucleus scattering of $^{8}$B solar neutrinos, the signal from which is very similar to a dark matter particle with mass of about 5.5 GeV/$c^{2}$, which result in typical nuclear recoil energies of $<$5 keV$_{\text{nr}}$. Therefore, it is of crucial importance to calibrate the response of recoiling xenon nuclei to keV-energy recoils. This analysis details the first in situ photoneutron calibration of the LZ detector and probes its response in this energy regime.

Aalbers, J. [SLAC; Stanford U., Phys. Dept.; KIPAC↗

Entanglement Requirements for Coherent Enhancement in Detectors

Coherent enhancement is a powerful mechanism for improving the sensitivity of a wide range of detectors, but its practical use is often limited by the difficulty of preparing the required quantum states. We show that this difficulty has a fundamental origin: coherent enhancement of a signal interacting with a detector is quantitatively constrained by entanglement. We prove general bounds on how the strength of coherent effects can scale with system size, as a function of the single-mode entanglement entropy of the detector. These bounds smoothly interpolate between the incoherent and fully coherent regimes, and apply both to parameter-estimation problems and to scattering processes. We discuss these results from two complementary perspectives: First, they appear as bounds on the quantum Fisher information of many-body states, which translate directly into limits on parameter sensitivity via the quantum Cramér-Rao bound. Second, they can be interpreted as limits on a class of scattering cross sections, leading to predictions for how minimum detectable interaction strengths scale with target size. Together, these results provide a unified view of coherent enhancement in metrology and scattering experiments, and motivate the development of new techniques for generating entangled detector states.

Bogorad, Zachary [Fermilab] (ORCID:000000019913647↗

Aerobot Autonomy Architecture

An architecture for autonomous operation of an aerobot (i.e., a robotic blimp) to be used in scientific exploration of planets and moons in the Solar system with an atmosphere (such as Titan and Venus) is undergoing development. This architecture is also applicable to autonomous airships that could be flown in the terrestrial atmosphere for scientific exploration, military reconnaissance and surveillance, and as radio-communication relay stations in disaster areas. The architecture was conceived to satisfy requirements to perform the following functions: a) Vehicle safing, that is, ensuring the integrity of the aerobot during its entire mission, including during extended communication blackouts. b) Accurate and robust autonomous flight control during operation in diverse modes, including launch, deployment of scientific instruments, long traverses, hovering or station-keeping, and maneuvers for touch-and-go surface sampling. c) Mapping and self-localization in the absence of a global positioning system. d) Advanced recognition of hazards and targets in conjunction with tracking of, and visual servoing toward, targets, all to enable the aerobot to detect and avoid atmospheric and topographic hazards and to identify, home in on, and hover over predefined terrain features or other targets of scientific interest. The architecture is an integrated combination of systems for accurate and robust vehicle and flight trajectory control; estimation of the state of the aerobot; perception-based detection and avoidance of hazards; monitoring of the integrity and functionality ("health") of the aerobot; reflexive safing actions; multi-modal localization and mapping; autonomous planning and execution of scientific observations; and long-range planning and monitoring of the mission of the aerobot. The prototype JPL aerobot (see figure) has been tested extensively in various areas in the California Mojave desert.

Elfes, Alberto↗

Multi-technique characterization of iron reduction by an Antarctic Shewanella : an analog system for putative Martian biosignature identification

ABSTRACT Microbes from terrestrial extreme environments enable testing of biosignature production in conditions relevant to astrobiological targets. Mars, which was likely more conducive to life during early warmer and wetter epochs, has inspired missions that search for signs of early life in the surficial rock record, including mineral or organic biosignatures. Microbial iron reduction is a common and ancient metabolism that may have also operated on other rocky celestial bodies. To investigate biosignature production during iron reduction, aShewanellasp. (strain BF02_Schw) isolated from a subglacial discharge known as Blood Falls, Antarctica, was incubated with the electron acceptor ferrihydrite (Fh). Biosignatures associated with Fh reduction were identified using a suite of techniques currently utilized or proposed for Mars missions, including X-ray diffraction and infrared, Mössbauer, and Raman spectroscopy. The biotic origin of features was validated by transcriptional changes observed between treatments with and without Fh and comparison to killed controls. In live treatments, Fh was reduced to magnetite and goethite, both detected in Martian lacustrine basins. Several soluble and volatile metabolites were also detected, including riboflavin and dimethyl sulfide (DMS), which could be astrobiological indicators of active microbial processes. While none of the identified biosignatures individually would serve as definitive proof of life (past or present), detecting concomitant features associated with known terrestrial biotic processes would provide compelling rationale for more targeted life detection missions. Terrestrial extremophiles can support the exploration of astrobiologically relevant microbial processes, validation of life detection instrumentation, and potentially the discovery of new biomarkers. IMPORTANCE Culture-based experiments with terrestrial extremophiles can elucidate biosignatures that may be analogous to those produced under extraterrestrial conditions, and thus inform sampling and technology strategies for future missions. Here, we demonstrate the production of several biosignatures under iron-reducing conditions byShewanellasp. BF02_Schw, originally isolated from an Antarctic analog feature. These biosignatures could be detectable using flight-ready instrumentation. Growth experiments with terrestrial extremophiles can identify biosignatures measurable by current methodologies and inform the development and optimization of techniques for detecting extant or extinct life on other worlds.

Biotechnology & Applied Microbiology↗